On 14/08/2018 12:27, Careina Edgooms wrote:
Cryoprotectant was 50% PEG just added to the buffer that it
crystallised in. It crystallised under batch.
which kind of batch plate ? Some are not suitable to in situ.
JL
On Tuesday, August 14, 2018, 12:11:17 PM GMT+2, Hughes, Jon
<jon.hug...@bot3.bio.uni-giessen.de> wrote:
maybe it's the cryobuffer that's the problem (you didn't mention it).
you could try to fish the crystals with minimal liquid attached by
mounting them in oil rather than a cryobuffer. or you could test the
native diffraction "in situ" (at room temperature in the drop): quite
a few beamlines offer this possibility these days.
best
jon
*Von:*CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *Im Auftrag
von *Careina Edgooms
*Gesendet:* Dienstag, 14. August 2018 11:59
*An:* CCP4BB@JISCMAIL.AC.UK
*Betreff:* [ccp4bb] crystals that dont diffract :( :(
I got the most beautiful crystals I have ever seen and they don't
diffract at all. Not poor diffraction, NO diffraction. Anyone know why
this could be and how I can go about fixing it? I had three beautiful
crystals and not one diffracted. I did leave them in the drop for
about 3 weeks before harvesting and in liquid nitrogen for about a
month before diffracting. Could that be a factor? If I regrew more
beautiful crystals and diffracted straight away could that help?
Careina
------------------------------------------------------------------------
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
------------------------------------------------------------------------
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
------------------------------------------------------------------------
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
--
--------------------------------
Jean-Luc Ferrer
Institut de Biologie Structurale
71 Avenue des Martyrs
CS 10090
38044 Grenoble Cedex 9 - FRANCE
Ph.: +33 (0)4 57 42 85 22
Cell: +33 (0)6 89 45 13 57
email: jean-luc.fer...@ibs.fr
--------------------------------
########################################################################
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
