Hi Careina, Some other things to check for:
Where did you measure the crystal, on a home source or at a synchrotron? if at a synchrotron, try to put bit more flux and see whether you see any diffraction? Also check the beam and your crystal alignment. Rangana On Tue, Aug 14, 2018 at 12:15 PM, Andreas Forster <docandr...@gmail.com> wrote: > Hi Careina, > > if you don't have grey hair (available in the lab), you can still mount > crystals at room temperature. With a MiTeGen RT kit, very little skill is > required to test crystal diffraction or even collect entire data sets at > room temperature. > https://www.mitegen.com/product/micrort-room-temperature-starter-kits/ > > All best. > > > Andreas > > > > On Tue, Aug 14, 2018 at 12:48 PM, Harry Powell <0000193323b1e616-dmarc- > requ...@jiscmail.ac.uk> wrote: > >> Hi >> >> One other thing to try that someone with grey hair in your lab might know >> about - mount a "crystal" in a capillary and see if it diffracts at room >> temp. As long as you have a source and detector in your home lab, there's >> no need to go to a synchrotron and use their in situ facilities. >> >> There are those of us who would contend that if your sample doesn't >> diffract, then it isn't a crystal, no matter how nice it looks (though it >> might have been one once...)! >> >> Harry >> -- >> Dr Harry Powell >> Chairman of European Crystallographic Association SIG9 (Crystallographic >> Computing) >> >> >> >> >> On 14 Aug 2018, at 11:14, Elspeth Garman wrote: >> >> Yes, essential to test at room temperature without changing their buffer >> medium before getting worried! >> If they don’t diffract at RT, they are very very unlikely to diffract at >> cryotemperatures, whatever you do to them before hand! >> Best wishes >> Elspeth >> >> *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf >> Of *Hughes, Jon >> *Sent:* 14 August 2018 11:11 >> *To:* CCP4BB@JISCMAIL.AC.UK >> *Subject:* [ccp4bb] AW: [ccp4bb] crystals that dont diffract :( :( >> >> maybe it's the cryobuffer that's the problem (you didn't mention it). you >> could try to fish the crystals with minimal liquid attached by mounting >> them in oil rather than a cryobuffer. or you could test the native >> diffraction "in situ" (at room temperature in the drop): quite a few >> beamlines offer this possibility these days. >> best >> jon >> >> *Von:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK >> <CCP4BB@JISCMAIL.AC.UK>] *Im Auftrag von *Careina Edgooms >> *Gesendet:* Dienstag, 14. August 2018 11:59 >> *An:* CCP4BB@JISCMAIL.AC.UK >> *Betreff:* [ccp4bb] crystals that dont diffract :( :( >> >> I got the most beautiful crystals I have ever seen and they don't >> diffract at all. Not poor diffraction, NO diffraction. Anyone know why this >> could be and how I can go about fixing it? I had three beautiful crystals >> and not one diffracted. I did leave them in the drop for about 3 weeks >> before harvesting and in liquid nitrogen for about a month before >> diffracting. Could that be a factor? If I regrew more beautiful crystals >> and diffracted straight away could that help? >> Careina >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> >> >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
