Hi
Did you try them at room temp, in situ (straight in the plate). We
observe that time to time on our beamline, when just harvesting, not
mentioning cryo protection, is enough to loose all diffraction. It-s
rare but happens.
Regards
JL
On 14/08/2018 11:58, Careina Edgooms wrote:
I got the most beautiful crystals I have ever seen and they don't
diffract at all. Not poor diffraction, NO diffraction. Anyone know why
this could be and how I can go about fixing it? I had three beautiful
crystals and not one diffracted. I did leave them in the drop for
about 3 weeks before harvesting and in liquid nitrogen for about a
month before diffracting. Could that be a factor? If I regrew more
beautiful crystals and diffracted straight away could that help?
Careina
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Jean-Luc Ferrer
Institut de Biologie Structurale
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