Hey Careina I had once experience the same phenomenon. No diffraction at all. That did not make any sense to me. So i dipped the "empty" loop in the water, and flash froze the loop in liquid nitrogen. Once exposed to X-ray, there was no ice rings... Eventually, it turned out that system had a glitch, and there was no X-ray. True story!
Best wishes -Z Zaigham Mahmood Khan, PhD Icahn School of Medicine at Mount Sinai Department of Oncological Sciences 1470 Madison Avenue New York On Tue, Aug 14, 2018 at 5:58 AM, Careina Edgooms < 000002531c126adf-dmarc-requ...@jiscmail.ac.uk> wrote: > I got the most beautiful crystals I have ever seen and they don't diffract > at all. Not poor diffraction, NO diffraction. Anyone know why this could be > and how I can go about fixing it? I had three beautiful crystals and not > one diffracted. I did leave them in the drop for about 3 weeks before > harvesting and in liquid nitrogen for about a month before diffracting. > Could that be a factor? If I regrew more beautiful crystals and diffracted > straight away could that help? > Careina > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
