no, the stats file has partial-volume corrected estimates in it, so simply counting the number of voxels in the volume won't match it On Mon, 3 Aug 2015, Jonathan Plasencia wrote:

That is partly correct. The nii is not matching the stats file (which it is
my understanding the stats file is based off the mgz file. If this is the
case then yes to your first question).
I'll send a mgz file and its stats file directly to your personal email in a
minute:

fis...@nmr.mgh.harvard.edu

Best,

Jon

On Mon, Aug 3, 2015 at 1:18 PM, Bruce Fischl <fis...@nmr.mgh.harvard.edu>
wrote:
      and you are saying that aseg.nii does *not* match the voxel
      counts in aseg.mgz? That is surprising. Can you email me the
      aseg.mgz?

      On Mon, 3 Aug 2015, Jonathan Plasencia wrote:

            I've given it a try in which I do not include
            " --out_orientation RAS" for
            writting an nii file (see output below). In doing so
            the new aseg.nii file
            appears to be the exact same as the original nii
            files I created (i.e. the
            number of voxels for a given segmentation ID did not
            change between the old
            and new nii file and neither of these are matching
            the stats file). Do you
            have another suggestion for fixing this? I ask
            because some of the smaller
            structures can have up to a 30% change in the number
            of voxels representing
            a given structure (comparing the nii file to the
            stats file).

            r500711$ mri_convert aseg.mgz aseg.nii 
            mri_convert aseg.mgz aseg.nii 
            $Id: mri_convert.c,v 1.179.2.7 2012/09/05 21:55:16
            mreuter Exp $
            reading from aseg.mgz...
            TR=0.00, TE=0.00, TI=0.00, flip angle=0.00
            i_ras = (-1, 0, 0)
            j_ras = (0, 0, -1)
            k_ras = (0, 1, 0)
            writing to aseg.nii...



            On Fri, Jul 31, 2015 at 2:31 PM, Jonathan Plasencia
            <jplas...@asu.edu>
            wrote:
                  Ok, I will give this a look. Thank you
            everyone.Best,

            Jon


                  cheers
                  Bruce
                  On Fri, 31 Jul 2015, Jonathan Plasencia wrote:

                        This is what we did from terminal:

                        mri_convert aseg.mgz aseg.nii
                        --out_orientation RAS
                        mri_convert aseg.mgz aseg.nii
                        --out_orientation RAS 
                        $Id: mri_convert.c,v 1.179.2.7
            2012/09/05
                        21:55:16 mreuter Exp $
                        reading from aseg.mgz...
                        TR=0.00, TE=0.00, TI=0.00, flip
            angle=0.00
                        i_ras = (-1, 0, 0)
                        j_ras = (0, 0, -1)
                        k_ras = (0, 1, 0)
                        Setting output orientation to RAS
                        Reslicing using trilinear interpolation 
                        writing to aseg.nii...

                        ** I ran this command from within the
                        subject's mri folder.

                        Should we try this
            without  --out_orientation
                        RAS ?

                        Best,

                        Jonathan Plasencia
                        Graduate Student
                        NSF Graduate Research Fellow
                        School of Biological and Health Systems
                        Engineering
                        Arizona State University
                        Email: jplas...@asu.edu
                        Phone: (480)861-7017

                        On Fri, Jul 31, 2015 at 12:54 PM, Bruce
            Fischl
                        <fis...@nmr.mgh.harvard.edu>
                        wrote:
                              why are you specifying the output
                        orientation? That is probably
                              causing
                              resampling

                              On Fri, 31 Jul 2015,
                              Jonathan Plasencia wrote:

                              >
                              > We are using the following line:
                              >
                              >
                              > mri_convert aseg.mgz aseg.nii
                        --out_orientation RAS
                              >
                              >
                              >
                              > Best,
                              >
                              > Jonathan Plasencia
                              > Graduate Student
                              > NSF Graduate Research Fellow
                              > School of Biological and Health
                        Systems Engineering
                              > Arizona State University
                              > Email: jplas...@asu.edu
                              > Phone: (480)861-7017
                              >
                              > On Fri, Jul 31, 2015 at 12:31
            PM,
                        Lilla Zollei
                              <lzol...@nmr.mgh.harvard.edu>
                              > wrote:
                              >
                              >       Hi Jon,
                              >
                              >       What is the exact command
            that
                        you used for the below
                              described
                              >       conversion?
                              >
                              >       Lilla
                              >
                              >       On Fri, 31 Jul 2015,
            Jonathan
                        Plasencia wrote:
                              >
                              >       > I've converted the
            aseg.mgz
                        file to .nii (need data in
                              nii to
                              >       do some further work on
            the
                        data). However, the number
                              of voxels
                              >       for a given structure
            changes
                        when comparing the stats
                              file
                              >       (from freesurfer) to the
            created
                        .nii file. What is
                              happening
                              >       here? Is there a fix for
            this? I
                        would expect the number
                              of
                              >       > voxels for a given
            structure
                        should be the same
                              between the
                              >       stats file and the .nii
            file.
                              >       > Note: I get the voxel
            values
                        in the stats file from
                              the
                              >       NVoxels column. I get the
            voxel
                        values in the generated
                              .nii
                              >       file by using matlab to
            count
                        the number pixels with a
                              given
                              >       SegId value (i.e. given
                        intensity value).
                              >       >
                              >       >
                              >       > Best,
                              >       >
                              >       > Jon
                              >       >
                              >       >
                              >
                       
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