How are you generating the stats? The aseg.stats file uses partial 
volume correction...

On 08/03/2015 04:48 PM, Jonathan Plasencia wrote:
> That is partly correct. The nii is not matching the stats file (which 
> it is my understanding the stats file is based off the mgz file. If 
> this is the case then yes to your first question).
>
> I'll send a mgz file and its stats file directly to your personal 
> email in a minute:
>
> fis...@nmr.mgh.harvard.edu <mailto:fis...@nmr.mgh.harvard.edu>
>
> Best,
>
> Jon
>
> On Mon, Aug 3, 2015 at 1:18 PM, Bruce Fischl 
> <fis...@nmr.mgh.harvard.edu <mailto:fis...@nmr.mgh.harvard.edu>> wrote:
>
>     and you are saying that aseg.nii does *not* match the voxel counts
>     in aseg.mgz? That is surprising. Can you email me the aseg.mgz?
>
>
>     On Mon, 3 Aug 2015, Jonathan Plasencia wrote:
>
>         I've given it a try in which I do not include
>         " --out_orientation RAS" for
>         writting an nii file (see output below). In doing so the new
>         aseg.nii file
>         appears to be the exact same as the original nii files I
>         created (i.e. the
>         number of voxels for a given segmentation ID did not change
>         between the old
>         and new nii file and neither of these are matching the stats
>         file). Do you
>         have another suggestion for fixing this? I ask because some of
>         the smaller
>         structures can have up to a 30% change in the number of voxels
>         representing
>         a given structure (comparing the nii file to the stats file).
>
>         r500711$ mri_convert aseg.mgz aseg.nii
>         mri_convert aseg.mgz aseg.nii
>         $Id: mri_convert.c,v 1.179.2.7 2012/09/05 21:55:16 mreuter Exp $
>         reading from aseg.mgz...
>         TR=0.00, TE=0.00, TI=0.00, flip angle=0.00
>         i_ras = (-1, 0, 0)
>         j_ras = (0, 0, -1)
>         k_ras = (0, 1, 0)
>         writing to aseg.nii...
>
>
>
>         On Fri, Jul 31, 2015 at 2:31 PM, Jonathan Plasencia
>         <jplas...@asu.edu <mailto:jplas...@asu.edu>>
>         wrote:
>               Ok, I will give this a look. Thank you everyone.Best,
>
>         Jon
>
>
>               cheers
>               Bruce
>               On Fri, 31 Jul 2015, Jonathan Plasencia wrote:
>
>                     This is what we did from terminal:
>
>                     mri_convert aseg.mgz aseg.nii
>                     --out_orientation RAS
>                     mri_convert aseg.mgz aseg.nii
>                     --out_orientation RAS
>                     $Id: mri_convert.c,v 1.179.2.7 2012/09/05
>                     21:55:16 mreuter Exp $
>                     reading from aseg.mgz...
>                     TR=0.00, TE=0.00, TI=0.00, flip angle=0.00
>                     i_ras = (-1, 0, 0)
>                     j_ras = (0, 0, -1)
>                     k_ras = (0, 1, 0)
>                     Setting output orientation to RAS
>                     Reslicing using trilinear interpolation
>                     writing to aseg.nii...
>
>                     ** I ran this command from within the
>                     subject's mri folder.
>
>                     Should we try this without  --out_orientation
>                     RAS ?
>
>                     Best,
>
>                     Jonathan Plasencia
>                     Graduate Student
>                     NSF Graduate Research Fellow
>                     School of Biological and Health Systems
>                     Engineering
>                     Arizona State University
>                     Email: jplas...@asu.edu <mailto:jplas...@asu.edu>
>                     Phone: (480)861-7017 <tel:%28480%29861-7017>
>
>                     On Fri, Jul 31, 2015 at 12:54 PM, Bruce Fischl
>                     <fis...@nmr.mgh.harvard.edu
>         <mailto:fis...@nmr.mgh.harvard.edu>>
>                     wrote:
>                           why are you specifying the output
>                     orientation? That is probably
>                           causing
>                           resampling
>
>                           On Fri, 31 Jul 2015,
>                           Jonathan Plasencia wrote:
>
>                           >
>                           > We are using the following line:
>                           >
>                           >
>                           > mri_convert aseg.mgz aseg.nii
>                     --out_orientation RAS
>                           >
>                           >
>                           >
>                           > Best,
>                           >
>                           > Jonathan Plasencia
>                           > Graduate Student
>                           > NSF Graduate Research Fellow
>                           > School of Biological and Health
>                     Systems Engineering
>                           > Arizona State University
>                           > Email: jplas...@asu.edu
>         <mailto:jplas...@asu.edu>
>                           > Phone: (480)861-7017 <tel:%28480%29861-7017>
>                           >
>                           > On Fri, Jul 31, 2015 at 12:31 PM,
>                     Lilla Zollei
>                           <lzol...@nmr.mgh.harvard.edu
>         <mailto:lzol...@nmr.mgh.harvard.edu>>
>                           > wrote:
>                           >
>                           >       Hi Jon,
>                           >
>                           >       What is the exact command that
>                     you used for the below
>                           described
>                           >       conversion?
>                           >
>                           >       Lilla
>                           >
>                           >       On Fri, 31 Jul 2015, Jonathan
>                     Plasencia wrote:
>                           >
>                           >       > I've converted the aseg.mgz
>                     file to .nii (need data in
>                           nii to
>                           >       do some further work on the
>                     data). However, the number
>                           of voxels
>                           >       for a given structure changes
>                     when comparing the stats
>                           file
>                           >       (from freesurfer) to the created
>                     .nii file. What is
>                           happening
>                           >       here? Is there a fix for this? I
>                     would expect the number
>                           of
>                           >       > voxels for a given structure
>                     should be the same
>                           between the
>                           >       stats file and the .nii file.
>                           >       > Note: I get the voxel values
>                     in the stats file from
>                           the
>                           >       NVoxels column. I get the voxel
>                     values in the generated
>                           .nii
>                           >       file by using matlab to count
>                     the number pixels with a
>                           given
>                           >       SegId value (i.e. given
>                     intensity value).
>                           >       >
>                           >       >
>                           >       > Best,
>                           >       >
>                           >       > Jon
>                           >       >
>                           >       >
>                           >
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Douglas N. Greve, Ph.D.
MGH-NMR Center
gr...@nmr.mgh.harvard.edu
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