Re: [Histonet] FW: Microtome at home

[Joseph Saby via Histonet]

You will need to make sure all pertinent SOPs and EOPs are followed, as well as 
all safety guidelines/protocols. Just because it is not human tissue doesn't 
mean that it can't have its share of nasties. 
Joe Saby

Sent from Yahoo Mail on Android 
 
  On Thu, Apr 16, 2020 at 8:21 AM, Porter, Amy via 
Histonet wrote:   Make sure of insurance 
coverage and safety for the employee and that they are covered in case of 
injury - are they still clocking in and out in some fashion. just thinking 
in a bigger box.


From: Steven Crochiere via Histonet 
Sent: Thursday, April 16, 2020 6:36 AM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] FW: Microtome at home

Jaime,

I don't see a problem with a research setting. If it was patient care, CLIA 
would need to inspect the set up in the person home. The same goes for our 
pathologists who read slide at home.

Steve

-Original Message-
From: raestask via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, April 15, 2020 7:51 PM
To: Jamie Watson ; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome at home

I wouldn't think there would be any problem.Rae Staskiewicz HT(ASCP)Sent from 
my Verizon, Samsung Galaxy smartphone
 Original message From: Jamie Watson via Histonet 
 Date: 4/15/20  6:44 PM  (GMT-06:00) To: 
Histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome at home Hello 
all,Our pathologist has come up with the idea of sending a microtome and 
waterbath home to someone that cannot come to work due to COVID 19.  We are a 
research lab and work with mouse and rat tissue.  Does anyone know of any 
issues with doing this?  I have never heard of anyone cutting slides at home 
other than someone with a private business.Thank 
you.Jamie___Histonet mailing 
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Re: [Histonet] HSV-2 antibody - Cell Marque was not the problem

[Joseph Saby via Histonet]

 Beth-
I am proud of you for stepping up and correcting the record.
This could not be an easy thing to do in such a public forum, but it also 
essential.
I hope you have a wonderful holiday.
Joe Saby


On Wednesday, July 3, 2019, 2:33:16 PM EDT, O'Neil, Beth via Histonet 
 wrote:  
 
 Last week I posted an inquiry about having problems with Cell Marque's HSV-2 
polyclonal antibody.  I have to retract my statement that it was Cell Marque 
using a different antibody source.  After spending two weeks troubleshooting my 
stainer, troubleshooting the positive control slides, yelling at my Cell Marque 
rep, etc.  I found out that it was the positive control slide and not the 
antibody.  When I originally suspected the positive QC slides (from Cancer 
Diagnostics), I requested a different lot number.  This new lot also failed to 
show positive staining.  Long story short, another call to Cancer Diagnostics 
finally resulted in receiving confirmation that they are having problems with 
their HSV-2 control slides.  It was my misfortune to have opened a new box of 
QC slides at the same time as opening a new vial of HSV-2 antibody which 
resulted in two weeks of headaches.  I will say that Cell Marque/Millipore was 
very supportive through this.  So, for those of you who are using Cancer 
Diagnostics HSV-2 control slides, they are working on a resolution and will 
replace their "bad" slides.  I was told that the staining is "hit or miss, "  
hopefully you all have the "hit" slides.

Beth Oneil, WVU Medicine




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Re: [Histonet] Histonet Digest, Vol 184, Issue 1

[Joseph Saby via Histonet]

 The more important question is who the h*ll is Kelly Jordan.
Terri has established her competency and reputation in NSH and the Histonet for 
probably over 20 years.
You have done your company a great disservice.
People will remember you, probably not as you would wish.
Joe Saby, retired


On Tuesday, March 5, 2019, 11:42:30 AM EST, Mark Tarango via Histonet 
 wrote:  
 
 How about passing on to the department that could fix the issue?  Where's
the empowering innovation?

On Fri, Mar 1, 2019 at 10:35 AM Jordan, Kelley via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> More bad press in histonet...not sure if we should pass it on to
> Marketing??
>
> @Will, Who as Teri Blaud at  Holy Redeemer Hospital ? Teri is always
> always bashing us.
> Kelley Jordan
>
> Strategic Account Manager - SC, NC, TN and KY
>
>
> A Member of the Roche Group
>
> Ventana Medical Systems
>
> Mobile:  803.504.1135
> Customer/Technical Support: 1.800.227.2155
>
> kelley.jor...@roche.com
>
> www.ventana.com
>
>
>
> Empowering | Innovation
>
>
> Confidentiality Note: This message is intended only for the use of the
> named recipient(s) and may contain confidential and/or proprietary
> information. If you are not the intended recipient, please contact the
> sender and delete this message. Any unauthorized use of the information
> contained in this message is prohibited.
>
> histonet 
>
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Re: [Histonet] VIP issue

[Joseph Saby via Histonet]

 Gudrun-
Assuming this is a new issue with a recent reagent change, I suspect that the 
alcohol used for the clean cycle was not 100% (maybe 95%?).
The ability of your alcohol to hold the paraffin in solution is lost as the 
percentage of water increases.
I also expect your solvent was mostly saturated with paraffin.
Try changing your clean cycle reagents and see if the situation improves. I 
would do this before processing tissue again on this unit.

I hope this helps.
Joe Saby



On Thursday, October 11, 2018, 1:48:54 PM EDT, Gudrun Lang via Histonet 
 wrote:  
 
 Dear all!

I have a question for those, who are familiar with the VIPs from Sakura. 

Last time we changed the reagenses the cleaning-ethanol (96%) was very milky
and even was full of many small particles. It was a paraffin-soup.

What is the cause for such a case?  We have been using the organic solvent
(ShellSol) for decades as xylensubstitute. 

Maybe the quality has suffered and the ability of solving paraffin has
decreased. But are there other explanations? Maybe a malfunction of the
instrument?

 

Thanks in advance

Gudrun Lang

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Re: [Histonet] broken screw in MMA

[Joseph Saby via Histonet]

 Talk with the people at EXAKT technologies.  They have plastice specially 
designed just for samples like that.
Joe

On Friday, June 22, 2018, 1:47:38 PM EDT, Terri Braud via Histonet 
 wrote:  
 
 Just an idea.  Why not use SEM?  It seems like it would be so much easier.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

  2. MMA or Epoxy Embedding (Jessica Riggleman)

Date: Thu, 21 Jun 2018 18:59:47 +
From: Jessica Riggleman 
Subject: [Histonet] MMA or Epoxy Embedding
Hi Everyone,
I am trying to embed screws in plastic (in the hopes to see breakage in the 
screw, etc). Right now I use specifically a mixture of methyl methacrylate, 
poly methyl methacrylate, and benzoyl peroxide. However this usually takes a 
few weeks to embed/dry. I am looking for something a little faster (perhaps a 
week max?).

Thank You,
Jessica
Jessica Riggleman | Research Associate
Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue | Audubon, PA 19403
Ph: (610) 930-1800 ext. 2583 | Fax:



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Re: [Histonet] Supervising a histologist

[Joseph Saby via Histonet]

Eileen-
I think it would depend on what specific procedures both of them are signed off 
on.And it would be on specific procedures.Or things are very strange in that 
area of Histoland.

Joe Saby


  From: Eileen Akemi Allison via Histonet 

 To: Histonet  
 Sent: Wednesday, November 1, 2017 8:20 AM
 Subject: [Histonet] Supervising a histologist
   
Hello Histoland:

This may sound like a strange question, but I have my reasons.  Has anyone run 
into a situation where a OJT histology assistant supervises an HTL when the 
histology manager is absent?  Is it even legal?  Any feedback would be greatly 
appreciated.


Akemi Allison BS, HT/HTL (ASCP)
Pathology Manager
Monterey Bay GI Consultants Laboratory
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940

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Re: [Histonet] Mammary Tissue Processing & Sectioning Troubles

[Joseph Saby via Histonet]

Andrea-
I believe your tissue was not fully fixed.  Many labs think they know more than 
they do, so itis always a good idea to pin down exactly what they did to fix 
the tissue.  I am guessing they left thick 
blobs of tissue in an inadequate volume of formalin and expected that to do the 
trick.
I would deparaffinize and rehydrate the tissues, then place them back in an 
appropriate volume of formalin 
and fix them until you know they will be fixed.  If the sections are thick, cut 
them thinner for this fixation.  After 
reprossessing, these tissues should section much better.
Good luck!
Joe Saby, retired

 
  From: Andrea Calhoun via Histonet 
 To: "histonet@lists.utsouthwestern.edu"  
 Sent: Wednesday, September 27, 2017 3:49 PM
 Subject: [Histonet] Mammary Tissue Processing & Sectioning Troubles
   
Hi All!

I'm working with primate mammary tissue that was given to us from a  pathology 
group off campus. They say the tissue was fixed in NBF for at least two days, 
and sent to us in 70% EtOH, where it has sat for a couple months.  We are 
treating these samples as we would with human tissue.  After grossing, 
processing (14hr schedule), and sectioning (4-5um, high-profile blade)), I find 
the tissue is difficult/ near impossible to section.  Even after leaving the 
blocks on ice for 30+min, the tissue continuously mushes against the blade.  If 
I do get it to cut, multiple knife marks develop quickly and I find I am going 
through blades like crazy.  I am staying superficial incase the fix didn't 
penetrate  deep into the tissue.    So far I've only processed a few samples 
from a larger group to resolve these issues before processing the remainder.

Does anyone have any tips for processing or sectioning?  Besides grossing the 
tissue into smaller pieces, would it help to re-fix them overnight or an 
additional day? How would you tell if the issue is from fixation or 
insufficient clearing/infiltration of paraffin?

Any information would be appreciated!

Andrea Calhoun B.S., CEMT
Research Assistant 2, Schedin Lab
Dept. of Cell, Developmental, & Cancer Biology
Oregon Health & Sciences University
calho...@ohsu.edu , RJH-5350

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Re: [Histonet] VIP5

[Joseph Saby via Histonet]

We had this happen when the individual changing the processor reversed order 
of the alcohols, placing the newest first and the oldest last.  Hope your 
answer 
is as simple, even if it is embarrassing.
Joe Saby

  From: "Abbott, Tanya via Histonet" 
 To: "histonet@lists.utsouthwestern.edu"  
 Sent: Wednesday, October 14, 2015 4:35 PM
 Subject: [Histonet] VIP5
   
Help!!! We changed our processor on Monday, all reagents. Run Monday into 
Tuesday a.m. was fine. Tuesday nights run had some blocks in it that weren't 
fully processed; chamber looked like it had droplets of residual water (or 
maybe alcohol?) in it. Dumped and refilled everything, ran a few test blocks, 
same scenario.
Any suggestions?!
Tanya

Tanya G. Abbott
Manager Technologist
Histology/Cytology
Penn State Health St. Joseph
(phone) 610-378-2635

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[Histonet] Joseph Saby

[Joseph Saby]

dshos http://bran-denschools.org/hui/jdul/jifh/pbiaq.htm
 Joseph Saby
 mhphq
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Re: [Histonet] Processing Guinea Pig

[Joseph Saby]

Heather-
 
I do not know why, but to properly process guinea pig tissues you need a much 
more rigorous program than what would work for mice. It is very easy to over 
process mouse tissue.  Even rat tissue needs more processing.  Guinea pig 
tissue need a program designed for processing larger animals/tissues, such as 
one would use to process dogs or even swine.
 
Let me know what programs you have, and I will get back with you with what 
would work. 
 
Joe Saby BA HT
NAMSA, Inc.
 


 From: Heather Marlatt hmarlat...@gmail.com
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
Sent: Monday, June 17, 2013 10:33 AM
Subject: [Histonet] Processing Guinea Pig
  

Hello Histonet! I'm a long time reader first time poster. Does anyone have
experience processing guinea pig tissues? I have been processing kidney and
heart but it is consistently coming out mushy in the middle. The mouse
tissue comes out fine even when processed on the same run. I had the tissue
grossed in thinner (2.5mm) thinking that perhaps it was too thick but it
didn't seem to help. Also, it has been fixed in 10%NBF for several days.

I was just wondering if anyone else had similar problems with guinea pig?

I appreciate in advance any advice or tips.

Here is the protocol:

Formalin 1hr
70% etOH 1hr
95% 1hr
100% 30min
100% 1hr
100% 1hr
100%1hr
Clearify 1hr
Clearify  1hr
Clearify 1hr
Paraffin 1hr
paraffin 1hr


All under pressure and heat only on the paraffin.

Thanks
Heather
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Re: [Histonet] Expiry date of NBF

[Joseph Saby]

We use the expiration date on the formalin as the use by date.  By the time the 
solution would be out of date, the tissues should be very well fixed, so the 
formalin becomes just a holding solution.

Joe Saby
NAMSA





From: amitapan...@torrentpharma.com amitapan...@torrentpharma.com
To: histonet@lists.utsouthwestern.edu
Sent: Wed, May 18, 2011 12:29:58 AM
Subject: [Histonet] Expiry date of NBF

Dear Histonetters,

I require one clarification on declaration of  expiry date of 4% nutral 
buffer formalin (NBF) prepared in our lab ( from 40% solution) ? 

Do you declare the expiry date of this prepared solution ? If so what 
criteria do you follow for this?

Important to mention that  we have to archive these tissue in NBF for 5 
years as part of GLP toxicology. 

Your experience or view on these points are welcomed.

Amita
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Re: [Histonet] Update regarding question on Plastic Embedding

[Joseph Saby]

Mahesh-

If you have access to a sonicator, you can etch the slides by first soaking 
them 
in 1% formic acid in the sonicator for 5-10 minutes, then after a brief water 
rinse soak them in 50% ethanol for another 5-10 minutes in the sonicator.

You may have to work with the staining times, but you will find that many of 
your paraffin embedding stains will work.

Good luck!

Joe Saby, BA HT
NAMSA




From: Mahesh Polavarapu polavarapu.mah...@gmail.com
To: histonet histonet@lists.utsouthwestern.edu
Sent: Tue, April 12, 2011 8:25:30 PM
Subject: [Histonet] Update regarding question on Plastic Embedding

Looking for a protocol to visualize vascularization and collagen deposition
at the bone-tendon interface of a rabbit rotator cuff embedded in a plastic
system. Sections will be rather thick (~50um) b/c they are being made
through a titanium anchor. Using MMA with a cold-curing resin, Technovit
9100. Thanks in advance!

- Mahesh
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Re: [Histonet] Help

[Joseph Saby]

Kathy-

What do these cracks look like?  Are they arranged in a parallel manner?  Or do 
they have the appearance of the cracks seen in dry mud?

Parallel aligned cracks are often found in overprocessed small biospies.  These 
small samples become hard and brittle.  The impact of the tissue on the 
microtome blade during aggressive facing force these cracks deep into the 
tissue.  With care, patience and some luck you may be able to get beyond these 
cracks by soaking the blocks, facing with thin sections on a repeated basis.  
What will determine whether you are successful will be whether there is enough 
depth to your biopsy to allow you to get beyond the facing artifact.

If the cracks resemble dry earth, then we are looking at a fixation/processing 
issue.  These cracks do not appear in the tissue until the xylenes after slide 
staining.  If the tissues are not well fixed, then processing reagents will not 
be able to fully penetrate the tissues.  If possible, I would suggest you 
perform retrims on your tissue and process them normally.  They should have had 
enough time to fix when you do your retrims, and they should be fine.  In a 
hospital setting, this may not be possible.  You can deparaffinze and rehydrate 
your tissue samples by running them through your processor's cleaning program.  
Place them back in fixative for a while, then reprocess them.  


I have also seen this artifcat in tissue when (due to a processor malfunction) 
tissue samples were exposed to high heat during processing.  Look for blood 
cells being laked in the larger blood vessels (blood cells look like a 
homogenous mass rather than being able to cell boundaries).  Sometimes there 
will be small round black precitate granules over the affected tissue areas.  
You will need to determine the best course depending on the extent of the 
damage.  Extensive damage will probaly require retrims. 

I hope this helps.  Please get back with me if yoiu have any further questions.

Joe Saby, BA HT
37 years in histotechnology



From: Kathy Nelson kathyenel...@hotmail.com
To: Histonet histonet@lists.utsouthwestern.edu
Sent: Sun, December 19, 2010 1:25:54 AM
Subject: [Histonet] Help


Solutions for cracks in tissue microscopically esp. in tumors and BCC specimens.
Thanks 
kathyenel...@hotmail.com                         
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Re: [Histonet] Bag Sealing system

[Joseph Saby]

Perhaps the best and least expensive tissue save bag sealer I have found can be 
purchased from ULINE.  There are benchtop models that easily fit in a hood, 
there are floor models.  The benchtop model we use cost less than $200 USD, 
easily fits in a hood, and has been totally trouble free for 2 years now.

I hope this helps.

Joe Saby, BA HT





From: WILLIAM DESALVO wdesalvo@hotmail.com
To: masterson_j...@allergan.com; histonet histonet@lists.utsouthwestern.edu
Sent: Wed, September 29, 2010 3:25:40 PM
Subject: RE: [Histonet] Bag Sealing system


Cardinal sells the Kapac sealer and a variety of sizes of bags at 2 mm  4 mm 
bags. This system is industrial and intended for laboratory use. We use to 
store 
tissue on-site and transport tissue and cassette between sites. has reduced 
storage space and incidence of fluid spills.

William DeSalvo, B.S., HTL(ASCP)





 From: masterson_j...@allergan.com
 To: histonet@lists.utsouthwestern.edu
 Date: Wed, 29 Sep 2010 10:26:24 -0700
 Subject: [Histonet] Bag Sealing system
 
 Hello,
 
 Can anyone recommend a bag and heat sealing system for archiving/shipping 
tissue? Thanks in advance.
 
 John
 
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Re: [Histonet] (no subject)

[Joseph Saby]

I knnow you can get vacuoles in rodent brains when processing them over the 
weekend with a hold station of 60-70% ethanol.  Other than that I cannot say.

Joe Saby, BA HT




From: Pathology patholog...@rccltd.in
To: histonet@lists.utsouthwestern.edu
Sent: Mon, August 30, 2010 7:07:22 AM
Subject: [Histonet] (no subject)

Hi ,

How to avoid vacuoles in the sections of brain and testes of rats. Whether
it is the problem of fixing, processing or staining.



Thanks  Regards,

S.Naveen Babu

Technician - Pathology



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Re: [Histonet] Tissue-Tek VIP 2000-3000

[Joseph Saby]

Mike-

Although Sakura is no longer making parts for the VIP 2000 units, there are 
thousands still in use around the country.  Most companies that service these 
units have been taking them in trade as people upgrade their equipment, then 
using these units to provide parts for units in trouble along the way.  They 
are 
very dependable (with regular water rinses and periodic PM).

Sakura does still makes parts for and service the VIP E300s, which is the step 
up from the 2000.  If the price were right, and funds were low, I would buy 
either. I have been using these units for many years.

Joe Saby, BA HT





From: Fimbres, Amber afimb...@uci.edu
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Fri, August 20, 2010 5:17:46 PM
Subject: [Histonet] Tissue-Tek VIP 2000-3000


Mike,

I know I'm a little late regarding your question about Sakura's VIP 2000/3000.  
I'm not sure if you found your answer, but if you're talking about the VIP 
processor that uses a magnet to program, change stations, etc. (and is reddish 
orange in color and may even say 'Miles' on it instead of Sakura) you will want 
to think twice before purchasing it.  These VIP K (series 1000, 2000, 3000) are 
no longer serviced by Sakura nor are there many parts for them.  Sakura's 
manufacturer for parts has stopped making replaceable parts for this particular 
model (they are at least 20 or more years old).  You can go to Sakura's website 
or call their technical support, they will confirm this too.

Take care,

Amber



  
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Re: [Histonet] decalcification

[Joseph Saby]

Dorothy-

If your bones are indeed large, 24 hours in 10% NBF will not be sufficient for 
fixation.  If the fixation isn't sufficient, none of the processes following 
that will be as effective as they might be.  Even after trimming, I would 
recommend fixing an additional 24-48 hours for every mm of thickness of your 
bone section, at a minimum.  If you are in a hospital setting and need reults 
quickly, you can use a processor (with heat, pressure and vacuum) to speed up 
the fixation.

I use buffered formic acid to decal.  The final concentration of formic acid is 
about 20-25%, so it is rather aggressive, but it also allows you to read the 
bone marrow after decal.  If you need results in a short time (hospital setting 
again) you can use an aggressive reagent like RDO for decal, but keep in mind 
that nuclear detail will be lacking, but bone structure should still be 
readable.  If you do not have the reagents to determine decal endpoints, you 
can 
(with training/practice) use a needle to pass through the sections to help 
determine if decal is complete.  The needle should move through the specimen 
smoothly and not get hung up and stiff in the middle of the section.  
Flexibility of the bone section can tell you when you are appoaching the time 
when the needle test will give you useable results.

Please do not read this as an endorsement for RDO or any other super aggressive 
decal solution.  Personally, I always insist on being able to read all tissue 
elements, and these solutions, although fast, give less than optimal staining 
results.

If you have further questions, please ask me off line.

Joe Saby, BA HT






From: Webb, Dorothy L dorothy.l.w...@healthpartners.com
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Mon, August 2, 2010 10:14:35 AM
Subject: [Histonet] decalcification

I would appreciate any feedback on what all are using in your decalcification 
process.  We get a lot of large bones in and the past 2-3 months have noticed a 
huge problem in our microtomy process with these samples.  We have been 
grossing 
the bones in and leaving the sample in the cassette in 10% formalin for 24 
hours 
befoere placing in decal for up to 8 hours and still having the inner portion 
of 
the sample look underprocessed and crunchy!  Any suggestions would be 
appreciated!

Dorothy Webb, HT
Regions Hospital Technical Supervisor
651-254-2962



  
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Re: [Histonet] Artifacts in histology section

[Joseph Saby]

What you are describing might be microchatter.  These will be sharp parallel 
lines/cracks that run parallel to the knife edge and are only visible under the 
microscope.

The usuall cause is a combination of overprocessing and rough facing that is 
too 
aggressive and/or with too dull a blade.  Overprocessing makes the tissue very 
hard and somewhat brittle.  The thick sections/dull knife cause the tissue to 
compress and then release, causing the chatter.  The actual danage is in the 
block face.

Once you have the problem in a block, if the tissue is thick enough, you might 
be able to repeatedly soak the block in ice water and gently (with a fairly 
sharp knife) reface.  With luck, you might be able to get through the damaged 
block face.  


Another artifact I have seen is similar, but the chatter appears very blurry.  
This is usually caused be poor fixation/processing, then oversoaking the blocks 
after facing.  The trick here is to reface the block, then chill it without 
exposure to water.  I've sectioned such blocks after placing them in a freezer 
to chill them thoroughly.  This will help to obtain a section, but may not fix 
the staining problems that might show up later.  


Good luck!

Joe Saby, BA HT





From: Aazath Raj aaz...@hotmail.com
To: histonet@lists.utsouthwestern.edu
Sent: Fri, July 23, 2010 11:26:28 AM
Subject: [Histonet] Artifacts in histology section



Dear Friends,

              I am an Histology Technologist. I am having a problem here,while 
sectioning am not seeing and scoring artifacts on the section but in the 
microscope am seeing a tearing artifacts particularly in endoscopy biopsies. am 
not able to locate where is the problem,is that because of blades or due to 
micro-crystallization of wax or due to any processing problem. Its not 
consistently in all but i get it on some blocks every  day. Can any one help me 
in sorting it out. If anybody is interested in will send the picture of those 
section.





with regards,

Aazathraj.P

Technical Officer,

Apollo Hospitals-chennai

India.

aaz...@hotmail.com


                        
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Re: [Histonet] re:VIP 2000

[Joseph Saby]

Sarah-

There is another possibility.  If you use formalin in the first station, you 
should rinse out the first 2 containers when changing the processor and fill 
them with warm water.  You can then perform a warm water flush.  This is 
setting 
up a program with 1 minute in each of the first 2 stations, causing the warm 
water to be pumped in and pumped out.  Residual formaldehyde can crystallize on 
the rotary valve and cause the issue you are now experiencing.  A service tech 
can remove the rotary valve, clean it and grease it, and get you back 
functioning.  This would be a part of what your preventative maintenance should 
be doing.

Good luck!

Joe Saby, BA HT





From: m...@techoneweb.com m...@techoneweb.com
To: histonet@lists.utsouthwestern.edu
Sent: Fri, July 9, 2010 2:31:34 PM
Subject: [Histonet] re:VIP 2000

Hey Sarah
The error code is a rotary valve error. Rotary valve does not stop at the
required station after 2 times. My guess is that you have a bad rotary
valve sensor board. It could also be the sensor disk.

Best
Matt Mincer
Tech One Biomedical Services
708-383-6040 X 10
www.techoneweb.com


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Re: [Histonet] Fume hood

[Joseph Saby]

Brandi-

Having gone through and even designed lab renovations, my advice is:

Have a different hood for each function if possible.  Renovations cannot see 
into the future to know what technologies you might need later.  An extra hood 
now can very easily seem like too few later.  Also, many functions need to be 
separated, a fact that administrators will never understand.  You might need 
vents over your processors, a vented area for special stains, a hood for 
grossing and a hood for stainers/ coverslippers.  Cytology may need several 
hoods also.  If genetic determination requirements become standard, will this 
be in your lab?

Emergency power for each hood.  Also processors and other necessary equipment 
(stainers, etc.).

Desktop height for many histology applications, especially grossing, embedding 
and sectioning.  Otherwise, the ergonomics are terrible.

I would suggest backdraft enclosed hoods for grossing, vented covered areas for 
processors, stainers/coverslippers.  If using xylenes, enclose as much as 
possible and use backdraft.  Be sure to keep in mind that with backdraft hoods, 
there may need to be face opening restrictions to achieve the majic number of 
100 cfm you need for safety.

If you have anyother questions, please get back with me.

Joe Saby, BA HT




From: Walter Benton wben...@umm.edu
To: histonet@lists.utsouthwestern.edu
Sent: Tue, June 15, 2010 12:49:25 PM
Subject: [Histonet] Fume hood

Brandi,

I have been through several renovations and highly recommend that you get a 
hood that exhaust outside. Depending upon what applications you plan to carry 
out under the hood, filters may not do the trick. It is also important to have 
someone from your facilities or engineering department conduct an air exchange 
analysis to make sure that you have proper airflow and exchanges within the 
room, since the two operations are being combined. I would also recommend that 
you have your area monitored for air quality while performing the various tasks 
that you perform now in you current configuration to see if you are OSHA 
compliant or whatever regulatory body you wish to follow. Downdraft ventilation 
is great for Xylene, because Xylene vapor is heavier than air, thus the reason 
those systems work well. Feel free to reach out if you have any other questions.

Walter Benton, HT(ASCP)QIHC
Histology Supervisor
University of Maryland Medical Center
Anatomic Pathology
22 S. Greene St 
Room NBW65
Baltimore MD 21201
(Direct) 410-328-0930
(Lab) 410-328-5524
(Fax) 410-328-5508


 On 6/15/2010 at 12:31 PM, histonet-requ...@lists.utsouthwestern.edu wrote:

Message: 13
Date: Tue, 15 Jun 2010 09:51:04 -0400
From: Brandi Higgins brandihigg...@gmail.com
Subject: [Histonet] fume hood
To: histonet@lists.utsouthwestern.edu 
Message-ID:
aanlktiml4gbsge6c_gbngaa1qcdfwtvqq4ueyxxah...@mail.gmail.com
Content-Type: text/plain; charset=ISO-8859-1

Hello,

Our hospital is doing some renovation and we need to look into new fume
hoods for our new location.  Currently we have one fume hood over our
grossing area, and one fume hood in our coverslipping area (two different
rooms).  The hospital wants to put our grossing room and histo/cyto rooms
together.  I am still going to need two separate hoods.  Does anyone have
any experience/knowledge/input about fume hoods?  I'm trying to look into
the ductless ones, although I imagine changing the filters will end up being
more expensive over time (I have no idea what would be involved in running a
duct/vent).  Also I have seen a benchtop downdraft type that sucks the air
down, and does not have a top.  It is advertised as being good for xylene.
Does anyone use this in their coverslipping area?  Any input would be
greatly appreciated.  I'm pretty clueless on the whole issue.  I want to
make sure that what I get will be safe for me and my coworker as we will be
spending most of our day in this room.  Any input is appreciated!  Thank
You!

Brandi Higgins, BS, HT(ASCP)



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Re: [Histonet] Prolonged FFPE slide and block storage

[Joseph Saby]

Sam-

Are you storing sectioned slides?

What we do is to deparaffinize and coverslip sectioned but unstained slides.  I 
haven' had to go back, but I believe they will store that way a long time.

Joe Saby, BA HT





From: Perry, Samuel samuel_pe...@dfci.harvard.edu
To: histonet@lists.utsouthwestern.edu
Sent: Fri, May 14, 2010 4:16:06 PM
Subject: [Histonet] Prolonged FFPE slide and block storage

Hi All,
We have a growing need for storing slides. 

They need to be stored in a way that they won't become oxidized and loose their
antigenicity, since we use them for IHC.  

Any suggestions for inexpensive methods for prolonged storage of FFPE slides and
blocks is greatly appreciated.

Thanks!

Sam Perry

Research Technician 
Dana-Farber Cancer Institute
Boston, MA


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Re: [Histonet] need tips for cross-sectioning of cortical bone

[Joseph Saby]

Brett-

Most wrinkles in decalcified bone sections come from stretching of the 
decalified bone that occurs during the sectioning process.  I would suggest a 
rather simple solution.  Allowing the sections to flatten on the waterbath 
might take longer or a higher temperature.  If paraffin surrounding the bone 
section seems to be containing it, not allowing it to expand to eliminate the 
wrinkles, gently tease it off.  After all, you want the bone section, not the 
paraffin.  A room temperature water bath (or 30% EtOH) to lay out the 
sections on to tease out any wrinkles before transfering the sections to the 
warm waterbath may also help.

I hope this helps!

Joe Saby, BA HT





From: Adam . anonwu...@gmail.com
To: Connolly, Brett M brett_conno...@merck.com
Cc: histonet@lists.utsouthwestern.edu
Sent: Thu, April 22, 2010 11:12:26 AM
Subject: Re: [Histonet] need tips for cross-sectioning of cortical bone

Cutting bone is very hard, and I'm by no means an expert at it. Assuming the
blocks are properly fixed and decalcified, the best thing I've found is to
put the blocks at -20C for 5-10 mins to cool them, then right before you cut
them, rub a little ice water on the face of the block. That should help you
get some nice clean cuts. If the sections become hard to cut again, reapply
the ice water. If that stops working, back in the freezer they go.

Adam

On Thu, Apr 22, 2010 at 9:58 AM, Connolly, Brett M brett_conno...@merck.com
 wrote:

 A colleague is having trouble getting wrinkle-free sections of
 decalcified, paraffin embedded femur.

 Any tips??

 Thanks,

 Brett M. Connolly, Ph.D.
 Molecular Imaging Team Leader
 Merck  Co., Inc.
 PO Box 4, WP-44K
 West Point, PA 19486
 tel. 215-652-2501 fax. 215-993-6803
 brett_conno...@merck.com



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Re: [Histonet] Cleaning VIP Processor containers

[Joseph Saby]

Brandi-

The flush should be with warm water (you do not need hot), and should be for 
the fixative station(s) and the first alcohol.  You only need 1 minute in each, 
just enough to get the solution to pump in.

Joe Saby, BA HT





From: Anthony Reilly tony_rei...@health.qld.gov.au
To: Brandi Higgins brandihigg...@gmail.com; histonet@lists.utsouthwestern.edu
Sent: Mon, April 5, 2010 10:36:42 PM
Subject: Re: [Histonet] Cleaning VIP Processor containers

Hi Brandi

Do you do hot water flushes? If not the film will be a build up of the buffer 
salts from your formalin.  To flush replace your formalin containers with hot 
water and write a program to run each container for 10-15 minutes.  This will 
not only clean the retorts but the fluid lines as well.  This should be done 
weekly or at least monthly if time is an issue.

regards
Tony





Tony Reilly  B.Sc. , M.Sc.
Chief Scientist, Anatomical Pathology
Pathology Queensland-PA Laboratory
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Level 1, Building 15,Princess Alexandra Hospital
Ipswich Road,WOOLLOONGABBA  Qld4102
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Mob: 0402 139411
Fax: 07 3176 2930
Email: tony_rei...@health.qld.gov.au
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Re: [Histonet] mouse perfusion rate

[Joseph Saby]

All-

From previous work with rat perfusions, the flow rate was about 10 ml/minute.  
If I had to guess, the equivalent flow rate for a mouse would be closer to 1-3 
mls/10 minutes.  If you go 10 ml/minute, you will definitely cause blowout 
artefacts.

Joe Saby, BA HT





From: Merced M Leiker lei...@buffalo.edu
To: charles.scou...@leica-microsystems.com; mak...@ufl.edu; 
histonet@lists.utsouthwestern.edu
Sent: Fri, March 19, 2010 9:21:38 AM
Subject: RE: [Histonet] mouse perfusion rate

The vasculature will leak too much and the mouse will get bloated - you'll 
see it first in either the intestines blowing up like a balloon or fluid 
coming out of the nose. Just not the same as the heart pumping when the 
mouse is alive with intact physiology and normal functioning.  Don't know 
exactly why, but that's what happens when you go too fast.  Perhaps the 
vasculature has lost its control to compensate for the pressure? I'm not a 
physiologist so I'm not sure why...maybe someone on the Histonet can answer 
that?

Regards,
Merced

--On Thursday, March 18, 2010 5:49 PM -0500 
charles.scou...@leica-microsystems.com wrote:



 Why not?  What happens?  One would think the mammalian cardiovascular
 system could withstand physiological pressures and flow rates, at least
 for one lifetime?




 Cordially,

 Charles W. Scouten, Ph.D

 Product Manager, MNL

 Biosystems Division



 Leica Biosystems Richmond, Inc.
 5205 Route 12
 P.O. Box 528
 Richmond, IL 60071
 United States of America

 Telephone 630 964 0501

 facsimile +1 630 964 0576

 www.MyNeuroLab.com

 www.leica-microsystems.com



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 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced M
 Leiker lei...@buffalo.edu
 Sent: Thursday, March 18, 2010 12:38 PM
 To: MKing mak...@ufl.edu; histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] mouse perfusion rate



 That may be mouse cardiac output, but I can assure you, from experience,
 you do not want to perfuse at 17ml/min.

 Regards,
 Merced

 --On Thursday, March 18, 2010 1:32 PM -0400 MKing  mak...@ufl.edu
 wrote:

 Li,

 Mouse cardiac output seems to be about 17 ml/min (e.g.
 www.transonic.com/mice1.shtml), you probably want to try for that to
 keep  pressures close to physiological.
 A syringe pump is pretty inexpensive and probably all you need.

 Mike

 - Original Message -
 From: Li Zhang  dancingw...@yahoo.com
 Date: Wednesday, March 17, 2010 14:59
 Subject: [Histonet] question about mouse perfusion
 To: histonet@lists.utsouthwestern.edu

   My question is: can anyone give me a rough idea of how fast I
   should inject ( like ml/min). I think I've tried like 30 ml in 3
   min, and I suspect that it's too fast because I do observe
   tissue swelling sometimes.



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 Merced M Leiker
 Research Technician III
 Cardiovascular Medicine
 348 Biomedical Research Building
 State University of New York at Buffalo
 3435 Main St, Buffalo, NY 14214 USA
 lei...@buffalo.edu
 716-829-6118 (Ph)
 716-829-2665 (Fx)

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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


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Re: [Histonet] Removing Yellow color from slides refixed in Bouin's solution

[Joseph Saby]

Running tap water for 10 minutes should do the trick.

Joe Saby





From: Cynthia Pyse cp...@x-celllab.com
To: cscam...@uci.edu; HistoNet histonet@lists.utsouthwestern.edu
Sent: Tue, March 16, 2010 10:52:20 AM
Subject: RE: [Histonet] Removing Yellow color from slides refixed in Bouin's 
solution

I just place the slides into 80% ETOH for 2 minutes.
Cindy

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
cscam...@uci.edu
Sent: Monday, March 15, 2010 7:42 PM
To: HistoNet
Subject: [Histonet] Removing Yellow color from slides refixed in Bouin's
solution

Hi Histonet,

After placing slides in Bouin's for 1 hour at 56 degrees, I am finding it
very difficult to remove the yellow coloring. Rinses with water are not
doing the trick. Does anyone have some advice on how to bring the slides
back to a clear color so that I may proceed with the Masson Trichrome
procedure?

Thanks!
-Colin


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Re: [Histonet] staining questions

[Joseph Saby]

Betsy-

When staining for proteoglycans, a stain usually associated with Alcian Blue 
would be PAS.  Stain with Alcian Blue first, then perform the PAS.  A light 
hematoxylin usually completes the stain.

Joe Saby, BA HT





From: Molinari, Betsy bmolin...@heart.thi.tmc.edu
To: histonet@lists.utsouthwestern.edu
Sent: Tue, February 2, 2010 6:34:04 AM
Subject: [Histonet] staining questions

Good morning!

I will be staining vessels for proteoglycans using Alcian Blue. I was
going to use the 2.5pH  protocol with a Nuclear Fast Red counterstain.
Does anyone have any other suggestion? The investigator does not want
Movats.

Also, I normally use PTAH as my fibrin stain for clots but was thinking
that there may be something better. I found the Fraser-Lendrum but the
protocol stated that Zenkers was the best fixative and that is out of
the question. So again, any suggestions? Thank you in advance.



Betsy Molinari HT(ASCP)

Texas Heart Institute

Cardiovascular Pathology

6770 Bertner Ave

MC1-283

Houston,TX 77030-2607

832-355-6524

832-355-6812



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[Histonet] (no subject)

[Joseph Saby]

Robert-

The artifact you describe is almost always due to varying combinations of two 
issues:
    1) overprocessing the biopsies, making them tough, and
    2) too agressive facing of the blocks.  
Thick facing sections cause cracks deep in the tough tissue.

I have worked through these issues in the past, but patience is required.  Soak 
and chill the blocks repeatedly while facing in at normal sectioning 
thickness.  You will need to work through the area of the biopsies that have 
the cracks forced into their structure.  With luck, you will have enough good 
tissue deeper in the block to provide a good section.

Good luck!

Joe Saby, BA HT




From: Moody, Robert rmo...@ameripath.com
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Wed, January 13, 2010 11:08:53 PM
Subject: [Histonet] RE: Histonet Digest, Vol 74, Issue 12

Hi, All we are having problems with chatter in our biopsies their usually on 
the edge of tissue is the a problem with the cutting or in the handling of the 
tissue after it is removed from the patient like the biopsies being left out to 
dry or not put in formalin what are some of your experience with this..
Robert Moody HT ASCP




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[Histonet] Open Position

[Joseph Saby]

I have a full time position open for a histotech at NAMSA, located just south 
and east of Toledo in Northwood, Ohio.  This is a full time days position, and 
requires skills in embedding, sectioning, staining, etc.  Must be willing to 
work on animal tissues.  We work on many interesting specimens, and are always 
working to expand our capabilities.

NAMSA is a CRO that performs a great deal of medical device testing.  Please 
feel free to look us up on the internet.  If you have any questions, you can 
respond to me at this email address, or at js...@namsa.com.

Thanks!

Joe Saby, BA HT(ASCP)



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[Histonet] Eager's Method

[Joseph Saby]

Fellow Histonetters-

I could use some help.

I have a request to use a stain for sympathetic nerve fibers called Eager's 
that can be combined with Luxol Fast Blue / Crsyl Echt Violet.

I know many of you would recommend GFAP or S100, and you would be absolutely 
correct.  Unfortunately, that is not on the table here.

I thank in advance any of you who respond with the reference I need.

Joe Saby, BA HT



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Re: [Histonet] diff-quik stain

[Joseph Saby]

Mercedes Medical has a Quick Dipp stain that I have heard is equivalent, b ut 
much less expensive.  Or you cxan always use Wright stain or Methylene Blue.

Joe Saby, BA HT





From: Feher, Stephen sfe...@cmc-nh.org
To: mlbuk...@ucalgary.ca; histonet@lists.utsouthwestern.edu
Sent: Wednesday, September 9, 2009 2:50:05 PM
Subject: RE: [Histonet] diff-quik stain

Thermo Fisher has one called 3 Step Stain  good stain and if you want
to use Methanol as a fixative, rather than the one that would be ordered
as a kit, you can save money by only ordering the 3 Step Stain Solution
A and 3 Step Stain Solution B.  


Steve

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of maureen
bukhari
Sent: Wednesday, September 09, 2009 2:21 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] diff-quik stain

Does anyone out there in Histonet -land have a recipe to make my own
diff-quik stain or a place to buy it. Is it marketed under another name?

Thanks ahead,



Maureen  Bukhari

Phone: 403-210-6524

e-mail: mlbuk...@ucalgary.ca





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Re: [Histonet] Biological hood with grossing station

[Joseph Saby]

Although I really like MOPEK, another source for the East Coast would be TBJ.





From: Golden State Acrylic Designs gsacrylicdesi...@gmail.com
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, July 16, 2009 9:34:56 AM
Subject: [Histonet] Biological hood with grossing station

Is the a source for a biological hood with grossing station othe than
(Thermo-Fisher)
Thanks
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Re: [Histonet] Signs of good perfusion

[Joseph Saby]

I agree that the pale liver is a great sign.  However, if the lungs fill up 
fluid comes out the nostrels or mouth, then the needle is probably inserted too 
far and has gone into the pulmonary vein.

I hope this helps!

Joe





From: Merced M Leiker lei...@buffalo.edu
To: Thach, Dzung (NIH/NIAID) [E] thac...@niaid.nih.gov; 
histonet@lists.utsouthwestern.edu
Sent: Monday, June 29, 2009 12:07:04 PM
Subject: Re: [Histonet] Signs of good perfusion

Live going pale is a good sign, your tissues of interest going pale is an even 
better sign, but fluid coming out of the mouth (or even the nose, or 
additionally, any kind of bloating or swelling in the animal) is a bad sign. 
You may not be able to get good perfusion (pale tissues) if this happens before 
your tissues turn pale, as the pressure is too high causing fluid to leak out 
of the vasculatureideally you want to push the blood out through the the 
hole you made in the right atrium, not through the walls of the vessels. at 
what rate do you perfuse? if this happens a lot slow it down.

Hope this helps.

--On Monday, June 29, 2009 11:31 AM -0400 Thach, Dzung (NIH/NIAID) [E] 
thac...@niaid.nih.gov wrote:

 Hi Everyone!!
 
    I am perfusing CO2 euthanized 3 weeks old mice with PBS only.  I am
 nicking the upper right atrium of heart to collect the gushed out blood
 and then perfusing through ventricle using a 21G butterfly needle and
 peristaltic pump.  Sometimes I see the lungs swelling up and fluid comes
 out of mouth.  Occasionally, I see the liver fade to light pink.  Most of
 the time the paws become white.  But the brain and spinal cord (my
 tissues of interest) are always white, and seem to have been perfused.  I
 was wondering how to improve this to get more consistent good perfusions,
 and what signs should I look for to indicate good perfusion?
 
 Thanks much,
 
 Dzung
 NIAID
 
 
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Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY  14214
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


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[Histonet] Staining Controls

[Joseph Saby]

Hello to Histoland-

I have a question concerning staining controls.

I am currently working for a GLP/GMP lab.  Their use of staining controls 
requires the purchase of said controls from sources such as Histology Controls 
Systems (TM) where the manufacturer certifies that these controls are effective 
controls for the stain for which they are listed.

Now, call me old school, but it has always seemed to me that many controls have 
internal control structures.  For instance, it seems superfluous to have a 
myelin control for a Luxol Fast Blue/PAS stain.  Or perhaps muscle (cardiac or 
skeletal) for a Masson's Trichrome.  Arterial wall for a Verheoff's Elastin 
stain.  Etc. 

Where I have worked in the past, we were always looking for excellent examples 
of tissue samples which exemplified excellent staining for control slides 
for specifc stains.  My manager does not have a background in histology or 
pathology, and this concept is new to her.  Since she keeps a sharp eye on the 
bottom line, and staining control slides are ridiculously expensive, she is 
open to the suggestion that our lab should use our own control slides.  
However, she feels I should seek outside opinions/testimonials about the 
certification process for said house controls, especially as this process 
relates to a GLP/GMP facility.

This question should be good for a healthy debate.  I look forward to the 
coming suggestions!

Joe Saby, BA, HT(ASCP)
Supervisor of Histology
NAMSA, Northwood Ohio



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Re: [Histonet] owners manuals

[Joseph Saby]

I can personally attest that Ford must have been having a VERY bad day indeed.  
He has supplied excellent information to this list on many occasions to many 
people over many years, and until that listing has always been very courteous.

We've all had bad days.  I would suggest cutting him some slack.

You can never tell who you will need, or who will come to your rescue, when the 
chips are down.

Joe Saby





From: Paul Verden pver...@uropartners.com
To: histonet@lists.utsouthwestern.edu
Sent: Monday, March 30, 2009 9:49:30 AM
Subject: [Histonet] owners manuals

Personally, I would like to thank Ford the demon vendor Royer at
Minnesota Medical, Inc.for his extensive dissertation on the request for
assistance on finding manuals.  Our lab will be expanding in the near
future and will be in need of some used equipment.  It's good to know
the equipment vendors you would like to avoid.  



R. Paul Verden  



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Re: [Histonet] question of the day - embedding

[Joseph Saby]

Tracy-

Where I used to work (at a place that shall remain nameless), we always kept 
our tissue being embedded in hot paraffin in the holding chamber.  Most of my 
work has been with animal tissues.

Where I work now, we don't.  And I do bellieve you are right.  If the tissues 
remain in hot paraffin, the heat transfer rate is very high, and the tissues 
continue to cook even when the chamber temperature has been reduced (as close 
as feasible) to the melting point of the paraffin.  I have seen little effect 
on the tissues of longer-than-I-would-like time in the holding chamber without 
paraffin.  Without the paraffin, the tissues do not get that direct heat from 
the melted paraffin and survive delay much better.

In short, I agree with you.  Not keeping the tissue in hot paraffin does not 
only not damage those tissues, it allows more flexiblity in your embedding 
times.

Joe Saby, BA HT





From: Tracy Bergeron tracy.berge...@biogenidec.com
To: histonet@lists.utsouthwestern.edu
Sent: Tuesday, February 17, 2009 4:14:46 PM
Subject: [Histonet] question of the day - embedding

Hi all question/dilemma of the day.

        I have been of the view that the longer tissue sat in melted 
paraffin the harder it got, especially animal tissue.  So with that said, 
for the past nearly 10 years I have not used melted paraffin in the 
holding chamber of the embedding center.  I just keep the chamber warm, 
and work that way.  Thus keeping the tissue from continuing to cook and 
harden in the wax.

        Everyone else I am currently working with has never seen the 
method I use, and firmly believe that this causes harm to the tissues if 
they are not in paraffin.

        Thoughts ideas etc.  I am dying to know if I am the only one that 
worries about length of time that animal tissue sits in paraffin.

Thanks.

Sincerely,
Tracy E. Bergeron, B.S., HT, HTL (ASCP)
Associate Scientist III, Pathology
Comparative Pathology Laboratory
Biogen Idec
14 Cambridge Center
Cambridge, MA 02142
Direct:  617-914-1115
Fax:  617-679-3208
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Re: [Histonet] Tissue Processors (VIP-1000)

[Joseph Saby]

All-

I did say the unit was a real workhorse!  In all honesty, we did use ours hard 
for well over 10 years before we moved on the the newer VIP 2000s and VIP 3000s.

Unfortunately, we remember our last experiences better than the early ones.  We 
had many good years with our VIP 1000s.  It wasn't until the end that we had 
problems.  Perhaps our service rep wasn't very good at scouting out parts 
(something I suspected very strongly at the time).  Perhaps he was smelling the 
commission on a new processor. 

All of the later VIPs come with my very strong recommendation.  Very solid 
equipment, very dependable.  Very, very few interrupted runs, and most of these 
were operator error.  If Ford Royer says he can get parts, then I would 
certainly recommend the VIP 1000 as well.  Again, years ago I was told parts 
were unavailable. But, again, I think that was just the person servicing my 
unit.

Jos Saby



From: Ford Royer fro...@bitstream.net
To: histonet histonet@lists.utsouthwestern.edu
Sent: Thursday, January 22, 2009 12:36:57 PM
Subject: RE: [Histonet] Tissue Processors (VIP-1000)

I have been servicing the VIP-1000/2000/3000 (aka: K Series) tissue
processors for over 15 years.  They were manufactured new from approximately
1983 to 1993.  They were manufactured and private labeled for Miles
Scientific, Inc. by Sakura Finetek. In the time Miles sold them new, I
estimate that well over 10k units were placed.  In the 15+ years that I have
serviced them, I have rarely come across the problems that Joe describes.
It is true that the Retort Lid can become warped over time, and that brand
new replacement parts are no longer available from Sakura, but there are so
many units in the field, and many refurbished equipment companies with their
own used parts departments, that a used replacement lid (that is not warped)
is easily found.  As to the electronics package that Joe mentions, again, of
the hundreds that I have serviced over the years and to this very day, I
have never had an electronics package (PCB/CPU) fail.  It is true that the
Power Supply to the electronics package does have a limited life span and
will burn out over time (this may be what Joe experienced).  But the Power
Supply is a very common component and brand new replacement units are
readily available from the electronics supply market.  I am not saying that
Joe did not experience a failure of one specific solid state PCB/CPU... it
can happen.  But it is very rare and does not reflect the continued
performance of the thousands of units that are out there... either still in
continuous use from the original date of purchase, or serving a second life
as a refurbished unit.

If you would like further details of my experience with the K Series VIP
tissue Processor, please contact me off-List.

~ Ford

Ford M. Royer, MT(ASCP)
Histology Product Manager
Minnesota Medical, Inc.
7177 Madison Ave. W.
Golden Valley, MN 55427-3601
CELL:  612-839-1046
Phone:  763-542-8725
Fax:  763-546-4830
Web: http://www.minnesotamedical.com



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Re: [Histonet] Re: tissue processors

[Joseph Saby]

I worked with a VIP 1000 well over 20 years ago.  This is a dinosaur!

Issues that will come to haunt you:

    The retort lid will warp over time (if it isn't already warped).  Minor 
overtensioning of the clamps cause this.  Many years ago we were told that new 
lids were no longer available.

    The printed circuit boards failed on a regular basis.  Last I heard, they 
were no longer made.  

Perhaps an aftermarket parts are now available for these issues.  I remember 
these units were real work horses.  But they had no where near the bells and 
whistles you get with more modern units.

Good luck!

Joe





From: Atoska Gentry gent...@vetmed.auburn.edu
To: Histonet histo...@pathology.swmed.edu
Sent: Wednesday, January 21, 2009 5:30:54 PM
Subject: [Histonet] Re: tissue processors

hello, we are in the market for a new/replacement tissue processor. If you have 
experience and/or pertinent detailed info on any of the following  will you 
please share with me ASAP? *1.*ThermoShandon Citadel 2000 Tissue Processor, 
*2.* Leica TP 1020 Automatic Tissue Processor, and last but not least  *3.* VIP 
1000 Floor Tissue Processor. Thank you kindly. Atoska
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Re: [Histonet] decal solutions

[Joseph Saby]

All-

If you are planning on looking at the bone marrow or are going to be evaluating 
nuclei in the bone, then I would heartily recommend either using 5% formic 
acid, or, preferably, a buffered formic acid solution.  The buffer can either 
be sodium formate or sodium citrate (set to a pH of ~2.2-2.4), but in either 
case, you can be working with a concentration of 20-25% formice acid and still 
preserve nuclear detail.  This is not as fast as RDO, but depending on what you 
want to look at, it can definitely be worth the investment in time!

Joe Saby, BA HT






From: Michele Wich mw...@7thwavelabs.com
To: histonet@lists.utsouthwestern.edu
Sent: Tuesday, January 6, 2009 10:00:02 AM
Subject: [Histonet] decal solutions

Can anyone recommend a decalcifying solution that works well for large
animal (dogs, pigs, etc.) bones like femurs and sternums?

Thanks in advance for any input.


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Re: [Histonet] Dissolve plastic

[Joseph Saby]

Lee and Peggy-

We section these eyes all the time with the plastic in place.  

I would certainly suggest a gluaraldehyde fixative.  Straight NBF is a very 
poor fixative for the many tissues found in eyes, especially the retina.  If 
you contact me directly, we can talk off line.  Eyes have held a special 
fascination with me for many years.

Joe Saby
NAMSA





From: Lee  Peggy Wenk [EMAIL PROTECTED]
To: histonet@lists.utsouthwestern.edu
Sent: Monday, November 24, 2008 12:47:24 PM
Subject: [Histonet] Dissolve plastic



I Need Histonetters help.

We have an eye with a plastic part for the lens, which is a type of
telescope, I've been told.

According to the new pathologist, we need to dissolve out this plastic lens,
then he can gross the eye and we can process it into paraffin.

We've never had to dissolve plastic before. The eye is in 10% NBF right now.
I'm guess maybe something like chloroform? But what percent? For how long?
Before or after grossing?

Has anyone done this before, that can send a procedure to me? Thanks.


***

Peggy A. Wenk, HTL(ASCP)SLS
Program Director,  Schools of Histotechnology
Supervisor, Mortuary Services
Laboratory Safety Officer, Disaster Preparedness and Quality Assurance
Coordinator
Department of Anatomic Pathology, 100RO
William Beaumont Hospital
3601 W. 13 Mile Road
Royal Oak, MI 48073-6769
Work  248/898-9079
Fax      248/898-9054
E-mail  [EMAIL PROTECTED]
Web Page: http://www.beaumont.edu/alliedhealth 


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