Re: [Histonet] Microtome block holder

[Colleen Forster via Histonet]

You are very welcome!

Colleen

On Tue, Nov 7, 2023 at 4:02 PM Naira Margaryan 
wrote:

> Awesome, thank you so much Colleen, for such quick response,
> Naira
>
> On Tue, Nov 7, 2023 at 4:01 PM Colleen Forster  wrote:
>
>> Naira,
>>
>> Contact Lee Dickey with Ted Pella. They carry the whole line of large
>> block adapters for the Leica microtomes.
>>
>> lee_dic...@tedpella.com
>>
>> I have this set up and it works great.
>>
>> Colleen Forster HT(ASCP)QIHC
>>
>> On Tue, Nov 7, 2023 at 3:58 PM Naira Margaryan via Histonet <
>> histonet@lists.utsouthwestern.edu> wrote:
>>
>>> Hello,
>>>
>>> We are thinking to process the dog stifle joints. For that reason we will
>>> need large cassette/slides (3x2) and more importantly to find a large
>>> block
>>> holder for the Leica Microtome.
>>>
>>> Any advice and suggestions where to purchase all large parts would be
>>> appreciated.
>>>
>>> Thanks in advance,
>>> Naira
>>>
>> ___
>>> Histonet mailing list
>>> Histonet@lists.utsouthwestern.edu
>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>>
>>
>> --
>> Colleen Forster HT(ASCP)QIHC
>> BLS Histology and IHC Laboratory
>> Jackson Hall, Room 2-155
>> 321 Church St. SE
>> 
>> Minneapolis, MN 55455
>> 
>> 612-626-1930
>>
>>
>>
>>
>>
>>
>>

-- 
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930
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Re: [Histonet] Microtome block holder

[Naira Margaryan via Histonet]

Awesome, thank you so much Colleen, for such quick response,
Naira

On Tue, Nov 7, 2023 at 4:01 PM Colleen Forster  wrote:

> Naira,
>
> Contact Lee Dickey with Ted Pella. They carry the whole line of large
> block adapters for the Leica microtomes.
>
> lee_dic...@tedpella.com
>
> I have this set up and it works great.
>
> Colleen Forster HT(ASCP)QIHC
>
> On Tue, Nov 7, 2023 at 3:58 PM Naira Margaryan via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
>
>> Hello,
>>
>> We are thinking to process the dog stifle joints. For that reason we will
>> need large cassette/slides (3x2) and more importantly to find a large
>> block
>> holder for the Leica Microtome.
>>
>> Any advice and suggestions where to purchase all large parts would be
>> appreciated.
>>
>> Thanks in advance,
>> Naira
>>
> ___
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>>
>
> --
> Colleen Forster HT(ASCP)QIHC
> BLS Histology and IHC Laboratory
> Jackson Hall, Room 2-155
> 321 Church St. SE
> 
> Minneapolis, MN 55455
> 
> 612-626-1930
>
>
>
>
>
>
>
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Re: [Histonet] Microtome block holder

[Colleen Forster via Histonet]

Naira,

Contact Lee Dickey with Ted Pella. They carry the whole line of large block
adapters for the Leica microtomes.

lee_dic...@tedpella.com

I have this set up and it works great.

Colleen Forster HT(ASCP)QIHC

On Tue, Nov 7, 2023 at 3:58 PM Naira Margaryan via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello,
>
> We are thinking to process the dog stifle joints. For that reason we will
> need large cassette/slides (3x2) and more importantly to find a large block
> holder for the Leica Microtome.
>
> Any advice and suggestions where to purchase all large parts would be
> appreciated.
>
> Thanks in advance,
> Naira
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


-- 
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930
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Re: [Histonet] microtome pm

[Travis via Histonet]

Depends on the make and model, same way a classic car would need more upkeep
compared to 2022 model that is still under manufacturer warranty. 6 months
for an old microtome might be necessary 

Travis O’Brien
 
International Medical Equipment, Inc.
170 Vallecitos De Oro
San Marcos, CA 92069
 
800.543.8496  Phone
760.761.0859  Fax
www.imebinc.com

-Original Message-
From: Paula via Histonet  
Sent: Tuesday, June 28, 2022 1:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] microtome pm

Hi everyone,

 

How often do you PM your microtomes?  Currently, we have them done every 6
months but I'm thinking once a year is sufficient and I wanted to get some
input before we decide.

 

Thank you in advance,

Paula

Bio-Path Medical Group

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Re: [Histonet] microtome pm

[Cooper, Brian via Histonet]

Once a year is good for us.  Just an added thought: we always make sure they 
change the extension springs in the cassette clamp with each PM as well.  

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: Paula via Histonet  
Sent: Tuesday, June 28, 2022 1:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] microtome pm (EXTERNAL EMAIL)

CAUTION: BE CAREFUL WITH THIS MESSAGE* This email came from outside 
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Hi everyone,

 

How often do you PM your microtomes?  Currently, we have them done every 6 
months but I'm thinking once a year is sufficient and I wanted to get some 
input before we decide.

 

Thank you in advance,

Paula

Bio-Path Medical Group

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Re: [Histonet] microtome blades and wax

[Rathborne, Toni via Histonet]

Jessica,

What has Leica said about the problem? I have always found them to be 
responsive and able to provide suggestions. This would be especially true with 
a new instrument, still under warranty. We have never had any inconsistencies 
with our Autocut, which was purchased last spring.

Toni




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-Original Message-
From: Piche, Jessica via Histonet 
Sent: Friday, January 14, 2022 8:43 AM
To: Histonet 
Subject: [Histonet] microtome blades and wax


*** This is an External Email ***

Good Morning,

We have been using Leica Histocore Autocut microtomes for a few months and have 
been having inconsistency with cutting on them. We have tried so many different 
blades, high and low, and have tried changing the blade angle. Nothing is a 
perfect fix, and we feel like we are really having to work much harder to get a 
great section. We were wondering what everyone else uses. I was also wondering 
if the wax could be an issue. We use EM-400 for embedding. I'd appreciate 
anyone's thoughts.

Thank you and have a nice weekend!

Jessica

Jessica Piche, HT(ASCP)
Waterbury Hospital Histology Laboratory
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Re: [Histonet] microtome blades and wax

[P Sicurello via Histonet]

Hi Jessica,

Happy Friday. 

Do you use the same EM-400 paraffin for processing?  If you use different 
paraffins that might contribute to your sectioning problems.   

If the waxes are different the tissue and the rest of the block are not one 
continuous unit in the "eyes" of the knife. The waxes have different properties 
so they cut differently because the embedding wax does not infiltrate into the 
tissue but wraps around it like a blanket.  

I'm not sure if that's what your lab does but if it is I suggest using the same 
paraffin for processing and embedding. 

Sincerely,

Paula Sicurello

Sent from my iPhone

> On Jan 14, 2022, at 5:43 AM, Piche, Jessica via Histonet 
>  wrote:
> 
> Good Morning,
> 
> We have been using Leica Histocore Autocut microtomes for a few months and 
> have been having inconsistency with cutting on them. We have tried so many 
> different blades, high and low, and have tried changing the blade angle. 
> Nothing is a perfect fix, and we feel like we are really having to work much 
> harder to get a great section. We were wondering what everyone else uses. I 
> was also wondering if the wax could be an issue. We use EM-400 for embedding. 
> I'd appreciate anyone's thoughts.
> 
> Thank you and have a nice weekend!
> 
> Jessica
> 
> Jessica Piche, HT(ASCP)
> Waterbury Hospital Histology Laboratory
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Re: [Histonet] microtome part

[LEROY H BROWN via Histonet]

Anyone happen to have a spare low profile knife holder laying around?  

Looking for one to fit my MICROM MODEL HM 330.  

Happy to pay you.   It is for my histo tech.  She needs a replacement for
her microtome.

 

Thanks,

LeRoy Brown HT(ASCP) HTL

HCS

 

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Re: [Histonet] Microtome Recommendations

[Bacon, Charles via Histonet]

Hi Kelly,

We have tried several manufactures, as well as model types over the years. We 
have had sliding microtomes (Microm, no longer available), and rotary 
microtomes from, Leizt (old), AO (very old), and Leica. We have had several 
models from Leica and prefer their durability. If you are manual, you are hard 
pressed to find a more durable and reliable microtome than the "Leica RM2125 
RTS - The Essential Microtome" you can get the gross feed wheel in "American 
(toward you)" or "European (away from you)" so be careful to ask when ordering. 
If you are looking for Automatic we have 10 of the  RM2255 from Leica, which I 
think is now called "HistoCore AUTOCUT - Automated Rotary Microtome." These are 
great and several of my techs have adopted the use of a foot pedal to preserve 
their shoulders.  Good luck and happy shopping!

Best,

Chuck Bacon, HTL(ASCP)CM 
Supervisor Histology
Baystate Medical Center
361 Whitney Ave., Holyoke, MA 01040
Telephone: 413-322-4786  Fax: 413-322-4790
charles.ba...@baystatehealth.org

-Original Message-
From: Pairan, Kelly  
Sent: Tuesday, February 09, 2021 10:30 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtome Recommendations

Good Morning Histoland,
We're looking into requesting capital for a new microtome and we were wondering 
what other labs were using and would recommend.

Thanks for your feedback!
Kelly

Kelly Pairan,  BS, HT (ASCP)CM, QIHC (ASCP)
Histology Supervisor-Anatomic Pathology
Department of Pathology and Laboratory Medicine
Email:  kelly.pai...@nationwidechildrens.org
ph: 614-722-5414
fx: 614-722-3033



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Re: [Histonet] Microtome at home

[Patsy Ruegg via Histonet]

I have done it, but you are right, I had my own private business, not sure why 
it would be a problem, especially for research.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: Jamie Watson 
Sent: Wednesday, April 15, 2020 5:44 PM
To: Histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Microtome at home

Hello all,

Our pathologist has come up with the idea of sending a microtome and waterbath 
home to someone that cannot come to work due to COVID 19.  We are a research 
lab and work with mouse and rat tissue.  Does anyone know of any issues with 
doing this?  I have never heard of anyone cutting slides at home other than 
someone with a private business.

Thank you.

Jamie

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Re: [Histonet] Microtome at home

[raestask via Histonet]

I wouldn't think there would be any problem.Rae Staskiewicz HT(ASCP)Sent from 
my Verizon, Samsung Galaxy smartphone
 Original message From: Jamie Watson via Histonet 
 Date: 4/15/20  6:44 PM  (GMT-06:00) To: 
Histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome at home Hello 
all,Our pathologist has come up with the idea of sending a microtome and 
waterbath home to someone that cannot come to work due to COVID 19.  We are a 
research lab and work with mouse and rat tissue.  Does anyone know of any 
issues with doing this?  I have never heard of anyone cutting slides at home 
other than someone with a private business.Thank 
you.Jamie___Histonet mailing 
listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Re: [Histonet] Microtome Cleaning Company and What to do with Old Metal Blades

[Jessica Riggleman via Histonet]

Hello,

I send mine out to IMEB in California. You may be able to sell the blades on 
Ebay.

Thank you,
Jessica



_

Jessica Riggleman | Research Associate

Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue | Audubon, PA 19403
Ph: (610) 930-1800 ext. 2583 | Fax:

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-Original Message-
From: Michelle Aono [mailto:aono...@auburn.edu]
Sent: Wednesday, October 26, 2016 10:56 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtome Cleaning Company and What to do with Old Metal 
Blades

Can anyone suggest a good company that works on old microtomes?  Ours is not 
broken, it's just in need of some TLC.  Also, what is a reasonable price for 
such work (we have a RJ 2040)?

Also, we have a lot of the old metal blades that you have to sharpen.  Any 
suggestions on how to sell, donate, recycle such items?  Do people even use 
these anymore?  I would hate to have them thrown out.

Thanks!
Michelle (Shelly) Aono
~~~
Research Associate II
107B/124 Greene Hall
Auburn University, Dept of APP
Auburn, AL 36849
(334) 844-5594


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Re: [Histonet] Microtome Cleaning Company and What to do with Old Metal Blades

[Carol G Fields via Histonet]

Hi,
I have used Michael Dietrich and his team at Southeast Pathology Instrument 
Servicefor years.  They are the best and the nicest!
Carole

Carole Fields, HT (ASCP)
Lead Histotechnologist, Pathology Laboratory
Martin Luther King Jr. Community Hospital

1680 E. 120th Street
Los Angeles, CA 90059
O:424-338-8000 x 8341
cfie...@mlkch.org


-Original Message-
From: Drew Meyer via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, October 26, 2016 9:59 AM
To: Michelle Aono <aono...@auburn.edu>
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome Cleaning Company and What to do with Old 
Metal Blades

Michael Dietrich and his team at Southeast Pathology Instrument Service is 
outstanding!

www.southeastpathology.com<http://www.southeastpathology.com>

Thanks,
Drew Meyer, HT
Anatomic Pathology Independent Contractor

On Wed, Oct 26, 2016 at 10:56 AM, Michelle Aono via Histonet < 
histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> 
wrote:

> Can anyone suggest a good company that works on old microtomes?  Ours
> is not broken, it's just in need of some TLC.  Also, what is a
> reasonable price for such work (we have a RJ 2040)?
>
> Also, we have a lot of the old metal blades that you have to sharpen.
> Any suggestions on how to sell, donate, recycle such items?  Do people
> even use these anymore?  I would hate to have them thrown out.
>
> Thanks!
> Michelle (Shelly) Aono
> ~~~
> Research Associate II
> 107B/124 Greene Hall
> Auburn University, Dept of APP
> Auburn, AL 36849
> (334) 844-5594
>
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Re: [Histonet] Microtome Cleaning Company and What to do with Old Metal Blades

[Drew Meyer via Histonet]

Michael Dietrich and his team at Southeast Pathology Instrument Service is
outstanding!

www.southeastpathology.com

Thanks,
Drew Meyer, HT
Anatomic Pathology Independent Contractor

On Wed, Oct 26, 2016 at 10:56 AM, Michelle Aono via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Can anyone suggest a good company that works on old microtomes?  Ours is
> not broken, it's just in need of some TLC.  Also, what is a reasonable
> price for such work (we have a RJ 2040)?
>
> Also, we have a lot of the old metal blades that you have to sharpen.  Any
> suggestions on how to sell, donate, recycle such items?  Do people even use
> these anymore?  I would hate to have them thrown out.
>
> Thanks!
> Michelle (Shelly) Aono
> ~~~
> Research Associate II
> 107B/124 Greene Hall
> Auburn University, Dept of APP
> Auburn, AL 36849
> (334) 844-5594
>
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Re: [Histonet] microtome validation

[Jacox, Robert A. via Histonet]

Ann,

I would recommend validating manual microtomes more often then automatic. Any 
traditional gear driven microtome does change overtime as gears wear. This 
eventually will lead to the micron indicator on the microtome not reading what 
you are truly cutting. Automated, microtomes driven by stepper motors tend to 
remain accurate longer but you may want to document the accuracy every couple 
of years. 

To check calibration most service engineers will mount a micrometer to the 
microtome and measure the feed of the microtome as the hand wheel turns. The 
procedure generally takes 5 minutes. Check with your microtome manufacture to 
see if they can assist you.

Robert Jacox
Thermo Fisher Scientific
Commercial Marketing Manager 



Sent from my iPad

> On Jul 9, 2016, at 1:39 PM, Ann Specian via Histonet 
>  wrote:
> 
> Has anyone had to validate a microtome (manual/automatic).  If so, how did 
> you do it?
> Ann
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Re: [Histonet] microtome issues: thick and no sections (Tyrone Genade)

[Tyrone Genade via Histonet]

Hello,

Thanks to all who responded to my question. The solution, it seems was very
simple.

Several people reported similar experiences and issues with the specimen
clamp not fitting tightly and that I should clean the clamp or block. My
blocks are clean and I thought the clamp was too... but when I ran a set of
forceps along the inner, upper edge of the clamp a tiny blob of wax was
found. A test block cut normally.

I'm amazed a tiny piece of wax could cause so much  trouble!

Thanks to everyone who offered help.

Bye
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Re: [Histonet] Microtome suggestions

[Rene J Buesa via Histonet]

For large specimens you need a "horizontal" or sledge microtome. Leitz (Leica) 
manufactures the best, but you could try an OMS from Reichert although I am not 
sure they stll are manufactured because Leica swallowed Reichert some years 
ago.Another alternative, and probably even better than the Leitz (Leica), is a 
Spencer (American Optical) sliding microtome Model 860 For me this is the best 
all-around ever manufactured sliding microtome and not too long ago a saw one 
for sale in e-Bay. Why don't you try looking for it. It will be much cheaper 
than any modern horizontal microtome and far better than any new one.René J. 


 On Wednesday, October 7, 2015 9:08 AM, "Goetz, Freya E. via Histonet" 
 wrote:
   

 Good morning!
I was wondering if anyone has microtome opinions they would like to share. We 
have an ultramicrotome but I am looking for a microtome that could cut larger 
pieces of tissue embedded in plastic. I am hesitant to go with Leica because 
service is so expensive but I’m looking for honest opinions.

Thanks and have a nice day!

Freya
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Re: [Histonet] Microtome suggestions

[Goetz, Freya E. via Histonet]

Hi Rene and histonet,

Thank you for your suggestions. I must amend my earlier request. We have an 
ultra microtome from RMC and a Spencer 820 microtome. I would like to section 
epoxy embedded material that is larger than an ultramicrotome could handle but 
smaller than a sledge microtome. Sorry I wasn’t specific.

Thank you again!

Freya


On Oct 7, 2015, at 12:01 PM, Rene J Buesa 
> wrote:

For large specimens you need a "horizontal" or sledge microtome. Leitz (Leica) 
manufactures the best, but you could try an OMS from Reichert although I am not 
sure they stll are manufactured because Leica swallowed Reichert some years ago.
Another alternative, and probably even better than the Leitz (Leica), is a 
Spencer (American Optical) sliding microtome Model 860
For me this is the best all-around ever manufactured sliding microtome and not 
too long ago a saw one for sale in e-Bay.
Why don't you try looking for it. It will be much cheaper than any modern 
horizontal microtome and far better than any new one.
René J.



On Wednesday, October 7, 2015 9:08 AM, "Goetz, Freya E. via Histonet" 
> 
wrote:


Good morning!
I was wondering if anyone has microtome opinions they would like to share. We 
have an ultramicrotome but I am looking for a microtome that could cut larger 
pieces of tissue embedded in plastic. I am hesitant to go with Leica because 
service is so expensive but I’m looking for honest opinions.

Thanks and have a nice day!

Freya
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Re: [Histonet] Microtome suggestions

[Hawkins, Hal K. via Histonet]

The old Sorvall JB-4 microtome was designed for this purpose and works well.  
They are available at a reasonable price from several used equipment providers 
and on Ebay. 



From: Goetz, Freya E. via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Wednesday, October 07, 2015 11:07 AM
To: Rene J Buesa
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome suggestions

Hi Rene and histonet,

Thank you for your suggestions. I must amend my earlier request. We have an 
ultra microtome from RMC and a Spencer 820 microtome. I would like to section 
epoxy embedded material that is larger than an ultramicrotome could handle but 
smaller than a sledge microtome. Sorry I wasn’t specific.

Thank you again!

Freya


On Oct 7, 2015, at 12:01 PM, Rene J Buesa 
<rjbu...@yahoo.com<mailto:rjbu...@yahoo.com>> wrote:

For large specimens you need a "horizontal" or sledge microtome. Leitz (Leica) 
manufactures the best, but you could try an OMS from Reichert although I am not 
sure they stll are manufactured because Leica swallowed Reichert some years ago.
Another alternative, and probably even better than the Leitz (Leica), is a 
Spencer (American Optical) sliding microtome Model 860
For me this is the best all-around ever manufactured sliding microtome and not 
too long ago a saw one for sale in e-Bay.
Why don't you try looking for it. It will be much cheaper than any modern 
horizontal microtome and far better than any new one.
René J.



On Wednesday, October 7, 2015 9:08 AM, "Goetz, Freya E. via Histonet" 
<histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> 
wrote:


Good morning!
I was wondering if anyone has microtome opinions they would like to share. We 
have an ultramicrotome but I am looking for a microtome that could cut larger 
pieces of tissue embedded in plastic. I am hesitant to go with Leica because 
service is so expensive but I’m looking for honest opinions.

Thanks and have a nice day!

Freya
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Re: [Histonet] microtome blade

[Jay Lundgren via Histonet]

NEVER leave a blade in a microtome when not sectioning!

  Sincerely,

Jay A. Lundgren, M.S., HTL
(ASCP)

On Wed, Aug 19, 2015 at 9:12 AM, Zerfas, Patricia (NIH/OD/ORS) [E] via
Histonet histonet@lists.utsouthwestern.edu wrote:

 Dear Histologists,
 After you have completed sectioning for the day do you remove the blade?
 Is it safer to keep the blade locked in place?  Is it a safety issue?  Or
 is it a policy set by your workplace?

 Thanks,

 Patricia M. Zerfas
 National Institutes of Health
 Bldg 28A Room 112 MSC 5230
 9000 Rockville Pike
 Bethesda, MD  20895  USA
 (301) 496-4464
 (301) 402-1068

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Re: [Histonet] microtome stage

[Alan Bright]

Donna, I would take the powder coating off with a paint stripper, try 
sectioning, if happy have the holder electro plated which will be a conductive 
coating that will protect the base metal from corrosion. Cost under $20.
BR Alan Bright, BIC...Cambs..UK

Sent from my iPhone

 On 30 Sep 2014, at 02:53, Donna Maney dmane...@yahoo.com wrote:
 
 I have a Leica SM2000 microtome, circa 2004, that has recently developed an 
 issue with the dry ice tray. Up until this year, we've never had any problems 
 with the specimen adhering to the platform. But suddenly we cannot cut 
 specimens because they easily become dislodged. We use 30% sucrose to stick 
 the specimen to the dry ice tray. When we borrow a dry ice tray from another 
 lab, the problem goes away.
 
 Our dry ice tray is powder coated and we can see the the coating is rubbing 
 away, but it does not seem to be coming off with the specimen. Rotating the 
 tray so that the specimen is attached at a different location on the platform 
 does not help. We have cleaned the dry ice tray with hot soapy water, rinsed 
 with distilled water and then with ethanol. 
 
 Leica told me that the new trays do not have this powder coating, which makes 
 me think they know about a problem, but the person I spoke to on the phone 
 was not aware of one.
 
 I'd be glad to hear from anyone who has experienced the same thing or who has 
 suggestions. A new tray will cost $500-$1000 so I'd like to troubleshoot with 
 this one to the extent that it is appropriate.
 
 Thanks,
 Donna
 
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Re: [Histonet] Microtome calibration

[Pam Marcum]

We have a service contract with an annual PM.  The aligments and calibration 
are done then. 



Pam 




- Original Message -
From: Michael Ann Jones mjo...@metropath.com 
To: Ronda Mire rm...@cvpath.org, Denise Long denise.l...@uconn.edu 
Cc: histonet@lists.utsouthwestern.edu 
Sent: Wednesday, July 16, 2014 8:07:37 AM 
Subject: Re: [Histonet] Microtome calibration 

We do ours by hand? You mean line them up or PM? 
Michael Ann Jones, HT (ASCP) 
Histology Manager 
Metropath 
7444 W. Alaska Dr. #250 
Lakewood, CO 80226 
303.634.2511 
mjo...@metropath.com 




On 7/15/14, 1:55 PM, Ronda Mire rm...@cvpath.org wrote: 

Hi Denise, 
 
Check with the company that does your PM service.  This may be something 
that is already done during preventative maintenance. 
 
On Jul 15, 2014, at 3:02 PM, Long, Denise denise.l...@uconn.edu wrote: 
 
 Hi Everyone, 
 During a recent inspection we were asked by the inspection team to 
start calibrating our microtomes.  Anyone know of a service vendor that 
will do this? Referrals, including self-referrals would be greatly 
appreciated. 
 Thank, 
 
 Denise M. Long, MS, HTL (ASCP), QIHC 
 University of Connecticut 
 Dept. of Pathobiology and Veterinary Sciences 
 Connecticut Veterinary Medical Diagnostic Laboratory 
 61 N. Eagleville Road, Unit 3089 
 Storrs, Connecticut 06269-3089 
 (860) 486-0851 
 
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Re: [Histonet] Microtome calibration

[Ronda Mire]

Hi Denise,

Check with the company that does your PM service.  This may be something that 
is already done during preventative maintenance.

On Jul 15, 2014, at 3:02 PM, Long, Denise denise.l...@uconn.edu wrote:

 Hi Everyone,
 During a recent inspection we were asked by the inspection team to start 
 calibrating our microtomes.  Anyone know of a service vendor that will do 
 this? Referrals, including self-referrals would be greatly appreciated.
 Thank,
 
 Denise M. Long, MS, HTL (ASCP), QIHC
 University of Connecticut
 Dept. of Pathobiology and Veterinary Sciences
 Connecticut Veterinary Medical Diagnostic Laboratory
 61 N. Eagleville Road, Unit 3089
 Storrs, Connecticut 06269-3089
 (860) 486-0851
 
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Re: [Histonet] Microtome calibration

[Michael Ann Jones]

We do ours by hand? You mean line them up or PM?
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 7/15/14, 1:55 PM, Ronda Mire rm...@cvpath.org wrote:

Hi Denise,

Check with the company that does your PM service.  This may be something
that is already done during preventative maintenance.

On Jul 15, 2014, at 3:02 PM, Long, Denise denise.l...@uconn.edu wrote:

 Hi Everyone,
 During a recent inspection we were asked by the inspection team to
start calibrating our microtomes.  Anyone know of a service vendor that
will do this? Referrals, including self-referrals would be greatly
appreciated.
 Thank,
 
 Denise M. Long, MS, HTL (ASCP), QIHC
 University of Connecticut
 Dept. of Pathobiology and Veterinary Sciences
 Connecticut Veterinary Medical Diagnostic Laboratory
 61 N. Eagleville Road, Unit 3089
 Storrs, Connecticut 06269-3089
 (860) 486-0851
 
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RE: [Histonet] Microtome calibration

[Weems, Joyce K.]

Could they have meant an aligner -  something like this?

http://www.newcomersupply.com/products/microtome-cryostat-aligners?page=M#1130


Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Long, Denise
Sent: Tuesday, July 15, 2014 3:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtome calibration

Hi Everyone,
During a recent inspection we were asked by the inspection team to start 
calibrating our microtomes.  Anyone know of a service vendor that will do this? 
Referrals, including self-referrals would be greatly appreciated.
Thank,

Denise M. Long, MS, HTL (ASCP), QIHC
University of Connecticut
Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical 
Diagnostic Laboratory
61 N. Eagleville Road, Unit 3089
Storrs, Connecticut 06269-3089
(860) 486-0851

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Re: [Histonet] Microtome

[Ronda Mire]

Agree, Leica is the best hands down.

On Jun 3, 2014, at 5:37 PM, nmhi...@comcast.net wrote:

 If someone promised me a brand new FREE microtome and it wasn't a LEICA, I'd 
 tell them, no, thanks!.  I am not inclined to endorse any other brand and I 
 cannot imagine changing my mind about that. 
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Re: [Histonet] Microtome

[Tony Auge]

Of the three microtomes that you listed I would highly recommend the Thermo
Shandon Finesse. It's of superior quality than the Microms I have used.
Cutting is excellent and the fly wheel action is light yet fluid. You do
have to oil it manually once a month but it's easy and you can clean it out
in the process and keep it in good operating condition. I haven't used the
Titan 5000 but I have used slides from Tanner that were of poor quality and
I ended up returning them.

I'm cutting on a used Leica 2125 right now. It is functional but it does
skip when facing and block orientation is terrible. Getting used lab
equipment is always a gamble so make sure your supplier is pleasant to deal
with and offers a warranty. Not sure what your budget is but a new Finesse
isn't too expensive for a new microtome and I'm sure you will be very happy
with it.


Tony Auge HTL (ASCP) QIHC
Histology Supervisor - Chandler Pathology Services
Cell: (651) 373-4768
Email: tony.a...@gmail.com
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RE: [Histonet] Microtome

[Susan.Walzer]

Leica 2125

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Monday, June 02, 2014 10:44 PM
To: Sylvia Shockey
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome

Leica all the way 2025

Sent from my iPhone

 On Jun 2, 2014, at 5:19 PM, Sylvia Shockey 
 sylvia.shoc...@clinicalpathologyassoc.com wrote:
 
 
 Hello,
   My name is Sylvia Shockey I work in a histology lab. One of 
 our microtomes is not working properly and would appreciate some advice on 
 purchasing a new one or maybe refurbished.  I have had some quotes on a 
 Tanner scientific manual rotary microtome, titan 5000 . Thermo Shandon 
 Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with Thermo fisher has 
 new and used on both of these models. Help me please I don't want to buy a 
 clunker.  Thanks for your time.
 
 
 
 
 
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RE: [Histonet] Microtome

[steven Mello]

Leica is an outstanding product.  Highly recommend there microtome.

Steven Mello,HT(ASCP)


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Monday, June 2, 2014 10:44 PM
To: Sylvia Shockey
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome

Leica all the way 2025

Sent from my iPhone

 On Jun 2, 2014, at 5:19 PM, Sylvia Shockey
sylvia.shoc...@clinicalpathologyassoc.com wrote:
 
 
 Hello,
   My name is Sylvia Shockey I work in a histology lab. One
of our microtomes is not working properly and would appreciate some advice
on purchasing a new one or maybe refurbished.  I have had some quotes on a
Tanner scientific manual rotary microtome, titan 5000 . Thermo Shandon
Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with Thermo fisher has
new and used on both of these models. Help me please I don't want to buy a
clunker.  Thanks for your time.
 
 
 
 
 
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RE: [Histonet] Microtome

[Anne Murvosh]

Most companies will let you test run their newest microtomes. Let them
come to you and test for a week or so than pick the one you like best.
Anne

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sylvia
Shockey
Sent: Monday, June 02, 2014 2:20 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtome


Hello,
   My name is Sylvia Shockey I work in a histology lab.
One of our microtomes is not working properly and would appreciate some
advice on purchasing a new one or maybe refurbished.  I have had some
quotes on a Tanner scientific manual rotary microtome, titan 5000 .
Thermo Shandon Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with
Thermo fisher has new and used on both of these models. Help me please I
don't want to buy a clunker.  Thanks for your time.





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Re: [Histonet] Microtome

[Rene J Buesa]

When a microtome starts to act up the best solution is to give it an 
overhaul. If with the possibility of buying one, contact Leica or Sakura first. 
Both manufacture very reliable and durable instruments.
René J.  


On Tuesday, June 3, 2014 10:12 AM, Anne Murvosh amurv...@advancederm.net 
wrote:
  


Most companies will let you test run their newest microtomes. Let them
come to you and test for a week or so than pick the one you like best.
Anne

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sylvia
Shockey
Sent: Monday, June 02, 2014 2:20 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtome


Hello,
                   My name is Sylvia Shockey I work in a histology lab.
One of our microtomes is not working properly and would appreciate some
advice on purchasing a new one or maybe refurbished.  I have had some
quotes on a Tanner scientific manual rotary microtome, titan 5000 .
Thermo Shandon Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with
Thermo fisher has new and used on both of these models. Help me please I
don't want to buy a clunker.  Thanks for your time.





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RE: [Histonet] Microtome

[Bea DeBrosse-Serra]

Leica 2125

Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Monday, June 02, 2014 7:44 PM
To: Sylvia Shockey
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome

Leica all the way 2025

Sent from my iPhone

 On Jun 2, 2014, at 5:19 PM, Sylvia Shockey 
 sylvia.shoc...@clinicalpathologyassoc.com wrote:
 
 
 Hello,
   My name is Sylvia Shockey I work in a histology lab. One of 
 our microtomes is not working properly and would appreciate some advice on 
 purchasing a new one or maybe refurbished.  I have had some quotes on a 
 Tanner scientific manual rotary microtome, titan 5000 . Thermo Shandon 
 Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with Thermo fisher has 
 new and used on both of these models. Help me please I don't want to buy a 
 clunker.  Thanks for your time.
 
 
 
 
 
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Re: [Histonet] Microtome

[nmhisto]

If someone promised me a brand new FREE microtome and it wasn't a LEICA, I'd 
tell them, no, thanks!.  I am not inclined to endorse any other brand and I 
cannot imagine changing my mind about that. 
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Re: [Histonet] Microtome

[Sue]

Leica all the way 2025

Sent from my iPhone

 On Jun 2, 2014, at 5:19 PM, Sylvia Shockey 
 sylvia.shoc...@clinicalpathologyassoc.com wrote:
 
 
 Hello,
   My name is Sylvia Shockey I work in a histology lab. One of 
 our microtomes is not working properly and would appreciate some advice on 
 purchasing a new one or maybe refurbished.  I have had some quotes on a 
 Tanner scientific manual rotary microtome, titan 5000 . Thermo Shandon 
 Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with Thermo fisher has 
 new and used on both of these models. Help me please I don't want to buy a 
 clunker.  Thanks for your time.
 
 
 
 
 
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RE: [Histonet] Microtome Service

[joelle weaver]

Thanks for this information Joyce. 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
From: joyce.we...@emoryhealthcare.org
To: Histonet@lists.utsouthwestern.edu
Date: Tue, 17 Dec 2013 23:03:02 +
CC: 
Subject: [Histonet] Microtome Service

Hello Folks,
 
I know that you know that every time someone mentions Leitz or Leica problems 
with a microtome or a cryotstat, I send out the name of Klaus Dern, my good 
friend for so many years I've lost count.
 
I want to let you know about something he can do that save your labs a lot of 
money, if you want service instead of replacing expensive parts because the one 
present on the newer microtomes can not be repaired.
 
For the following microtomes,
Leica RM2125
Leica 2030 Biocut
Leica/Jung 2035
Leica CM-1850 Cryostat
Sakura SRM200
 
The advance mechanism can not be repaired, according to the company. Klaus can 
retool or whatever you call it,  the advance mechanism. Excessive play can 
develop in the advance mechanism, causing chunking of the block and the agony 
and gnashing of teeth. The company will tell you the whole part needs to be 
replaced or perhaps you even need to get a new microtome.
 
Micro Precision Instruments Co (none other than Klaus Dern) can repair the 
instrument for about half of what the new part would cost.  The best thing 
about the repair is that it becomes adjustable and can forever work with little 
maintenance.
 
I just wanted to share this with you - I am not the vendor. I just want to let 
you know of something that can help in these trying times of budget constraints 
and replacing equipment. He can repair the microtomes out of the cryostats. You 
can remove and send to him. He has loaners (I asked).   I just had a cryostat 
repaired. He is close enough to me that he can come on site and I don't need to 
ship.
 
Here is his info for those who might be interested.
Phone: 706-635-8840
Fax: 706-635-3074
klaus.der...@gmail.com
 
Hope you find this helpful!
 
Hope everyone is enjoying the hustle and bustle of the season. Merry Christmas, 
ya'll!!   j
 
 
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
 
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
 
This e-mail, including any attachments is the property of Saint Joseph’s 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.
 

 
This e-mail message (including any attachments) is for the sole use of
the intended recipient(s) and may contain confidential and privileged
information. If the reader of this message is not the intended
recipient, you are hereby notified that any dissemination, distribution
or copying of this message (including any attachments) is strictly
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If you have received this message in error, please contact
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Re: [Histonet] Microtome Blade safety, in or out when not in use?

[Paula Sicurello]

Left in but covered with the blade guard.  Not spanking new, but usable
(for facing) get stored in an old box that the slides came in.  I like the
slide mailer idea, and will switch to that.

-- 
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory
Duke University Health System
Rm.#251M, Duke South, Green Zone
Durham, North Carolina 27710
P:  919.684.2091

HIPAA Privacy Notification: This message and any accompanying documents are
covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521,
and contain information intended for the specific individual (s) only. This
information is confidential. If you are not the intended recipient or an
agent responsible for delivering it to the intended recipient, you are
hereby notified that you have received this document in error and that any
review, dissemination, copying or the taking of any action based on the
contents of this information is strictly prohibited . If you have received
this communication in error, please notify us immediately by e-mail, and
delete the original message.

On Thu, Oct 24, 2013 at 9:33 PM, Leah Simmons leah_simmon...@hotmail.comwrote:

 Hello all :-)
 I am doing a quick microtome blade safety survey,
 When you finish work, do you leave your blade in the microtome behind the
 blade guard or do you take it out?
 If you take it out and it is a new blade or a blade still useful for
 trimming  where do you store it?
 Thank you for your feedback, I really appreciate it.
 Regards
 Leah Simmons

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 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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RE: [Histonet] Microtome Blade safety, in or out when not in use?

[McAnn, Sherrian]

I was taught that when leaving your microtome for any length of time to
always take the blade out. We had a tech that had the habit of leaving
the blade on her microtome and even though she had the safety guard up
someone from  biomed still  managed to lean on it and get cut (go
figure)
   If I want to save a slightly used blade to maybe trim with the
next time then I will put it into a slide mailer (plastic with attached
lid) but that is a safety issue .  The safety officer says that when a
blade is used and taken out then it should be thrown away..because the
more it is being handled the greater the chance of injury. Just saying
what they told me.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula
Sicurello
Sent: Friday, October 25, 2013 6:03 AM
To: Leah Simmons
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome Blade safety, in or out when not in
use?

Left in but covered with the blade guard.  Not spanking new, but usable
(for facing) get stored in an old box that the slides came in.  I like
the slide mailer idea, and will switch to that.

--
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory Duke University
Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina
27710
P:  919.684.2091

HIPAA Privacy Notification: This message and any accompanying documents
are covered by the Electronic Communications Privacy Act, 18 U.S.C.
2510-2521, and contain information intended for the specific individual
(s) only. This information is confidential. If you are not the intended
recipient or an agent responsible for delivering it to the intended
recipient, you are hereby notified that you have received this document
in error and that any review, dissemination, copying or the taking of
any action based on the contents of this information is strictly
prohibited . If you have received this communication in error, please
notify us immediately by e-mail, and delete the original message.

On Thu, Oct 24, 2013 at 9:33 PM, Leah Simmons
leah_simmon...@hotmail.comwrote:

 Hello all :-)
 I am doing a quick microtome blade safety survey, When you finish 
 work, do you leave your blade in the microtome behind the blade guard 
 or do you take it out?
 If you take it out and it is a new blade or a blade still useful for 
 trimming  where do you store it?
 Thank you for your feedback, I really appreciate it.
 Regards
 Leah Simmons

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 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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RE: [Histonet] Microtome Blade safety, in or out when not in use?

[Marcum, Pamela A]

The rule here is a blade is cheaper than a cut.  Anytime you are walking away 
and returning within a few minutes to cut use the knife guard otherwise throw 
the blade out.  It is an accident looking to happen.  Recently we had a tech 
decide not use the knife guard and seriously cut himself by misjudging the 
distance from his elbow reaching an knife he was no longer able to use.  

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McAnn, Sherrian
Sent: Friday, October 25, 2013 8:46 AM
To: Paula Sicurello; Leah Simmons
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Microtome Blade safety, in or out when not in use?

I was taught that when leaving your microtome for any length of time to always 
take the blade out. We had a tech that had the habit of leaving the blade on 
her microtome and even though she had the safety guard up someone from  
biomed still  managed to lean on it and get cut (go
figure)
   If I want to save a slightly used blade to maybe trim with the next time 
then I will put it into a slide mailer (plastic with attached
lid) but that is a safety issue .  The safety officer says that when a blade is 
used and taken out then it should be thrown away..because the more it is being 
handled the greater the chance of injury. Just saying what they told me.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello
Sent: Friday, October 25, 2013 6:03 AM
To: Leah Simmons
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome Blade safety, in or out when not in use?

Left in but covered with the blade guard.  Not spanking new, but usable (for 
facing) get stored in an old box that the slides came in.  I like the slide 
mailer idea, and will switch to that.

--
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory Duke University Health 
System Rm.#251M, Duke South, Green Zone Durham, North Carolina
27710
P:  919.684.2091

HIPAA Privacy Notification: This message and any accompanying documents are 
covered by the Electronic Communications Privacy Act, 18 U.S.C.
2510-2521, and contain information intended for the specific individual
(s) only. This information is confidential. If you are not the intended 
recipient or an agent responsible for delivering it to the intended recipient, 
you are hereby notified that you have received this document in error and that 
any review, dissemination, copying or the taking of any action based on the 
contents of this information is strictly prohibited . If you have received this 
communication in error, please notify us immediately by e-mail, and delete the 
original message.

On Thu, Oct 24, 2013 at 9:33 PM, Leah Simmons
leah_simmon...@hotmail.comwrote:

 Hello all :-)
 I am doing a quick microtome blade safety survey, When you finish 
 work, do you leave your blade in the microtome behind the blade guard 
 or do you take it out?
 If you take it out and it is a new blade or a blade still useful for 
 trimming  where do you store it?
 Thank you for your feedback, I really appreciate it.
 Regards
 Leah Simmons

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 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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RE: [Histonet] Microtome Blade safety, in or out when not in use?

[McAnn, Sherrian]

AMEN TO THAT!

-Original Message-
From: Marcum, Pamela A [mailto:pamar...@uams.edu] 
Sent: Friday, October 25, 2013 10:05 AM
To: McAnn, Sherrian; Paula Sicurello; Leah Simmons
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Microtome Blade safety, in or out when not in
use?

The rule here is a blade is cheaper than a cut.  Anytime you are
walking away and returning within a few minutes to cut use the knife
guard otherwise throw the blade out.  It is an accident looking to
happen.  Recently we had a tech decide not use the knife guard and
seriously cut himself by misjudging the distance from his elbow reaching
an knife he was no longer able to use.  

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McAnn,
Sherrian
Sent: Friday, October 25, 2013 8:46 AM
To: Paula Sicurello; Leah Simmons
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Microtome Blade safety, in or out when not in
use?

I was taught that when leaving your microtome for any length of time to
always take the blade out. We had a tech that had the habit of leaving
the blade on her microtome and even though she had the safety guard up
someone from  biomed still  managed to lean on it and get cut (go
figure)
   If I want to save a slightly used blade to maybe trim with the
next time then I will put it into a slide mailer (plastic with attached
lid) but that is a safety issue .  The safety officer says that when a
blade is used and taken out then it should be thrown away..because the
more it is being handled the greater the chance of injury. Just saying
what they told me.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula
Sicurello
Sent: Friday, October 25, 2013 6:03 AM
To: Leah Simmons
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome Blade safety, in or out when not in
use?

Left in but covered with the blade guard.  Not spanking new, but usable
(for facing) get stored in an old box that the slides came in.  I like
the slide mailer idea, and will switch to that.

--
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory Duke University
Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina
27710
P:  919.684.2091

HIPAA Privacy Notification: This message and any accompanying documents
are covered by the Electronic Communications Privacy Act, 18 U.S.C.
2510-2521, and contain information intended for the specific individual
(s) only. This information is confidential. If you are not the intended
recipient or an agent responsible for delivering it to the intended
recipient, you are hereby notified that you have received this document
in error and that any review, dissemination, copying or the taking of
any action based on the contents of this information is strictly
prohibited . If you have received this communication in error, please
notify us immediately by e-mail, and delete the original message.

On Thu, Oct 24, 2013 at 9:33 PM, Leah Simmons
leah_simmon...@hotmail.comwrote:

 Hello all :-)
 I am doing a quick microtome blade safety survey, When you finish 
 work, do you leave your blade in the microtome behind the blade guard 
 or do you take it out?
 If you take it out and it is a new blade or a blade still useful for 
 trimming  where do you store it?
 Thank you for your feedback, I really appreciate it.
 Regards
 Leah Simmons

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 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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RE: [Histonet] Microtome Blade safety, in or out when not in use?

[Morken, Timothy]

I always take it out. You never know if someone will come along and do 
something...

In our lab the mircrotomes do not strictly belong to any particular tech, and 
they are used by different people at different times of day or different 
shifts. 



Tim Morken
Department of Pathology
UC San Francisco Medical Center



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Leah Simmons
Sent: Thursday, October 24, 2013 6:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtome Blade safety, in or out when not in use?

Hello all :-)
I am doing a quick microtome blade safety survey, When you finish work, do you 
leave your blade in the microtome behind the blade guard or do you take it out?
If you take it out and it is a new blade or a blade still useful for trimming  
where do you store it?
Thank you for your feedback, I really appreciate it.
Regards
Leah Simmons
  
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Re: [Histonet] Microtome Blade safety, in or out when not in use?

[Jennifer MacDonald]

We save the blade to use for trimming.  We store the blade in a plastic 
5-slide mailer.



From:   Leah Simmons leah_simmon...@hotmail.com
To: histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edu
Date:   10/24/2013 06:36 PM
Subject:[Histonet] Microtome Blade safety, in or out when not in 
use?
Sent by:histonet-boun...@lists.utsouthwestern.edu



Hello all :-)
I am doing a quick microtome blade safety survey,
When you finish work, do you leave your blade in the microtome behind the 
blade guard or do you take it out?
If you take it out and it is a new blade or a blade still useful for 
trimming  where do you store it?
Thank you for your feedback, I really appreciate it.
Regards
Leah Simmons
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Re: [Histonet] Microtome advise...

[Jack Ratliff]

How about the new Sakura with the auto orientation feature! I saw it on display 
at the Missouri Society for Histotechnology meeting and it was quite 
impressive! Of course if you have the budget, there's alway the non-contact 
laser microtome from Rowiak! I have seen and used that unit to cut fresh soft 
tissues (brain) and resin embedded bone and biomaterial implants!

Jack


On Jul 18, 2013, at 7:57 AM, Tom McNemar tmcne...@lmhealth.org wrote:

 Hello all.  I am preparing my budget and am seeking recommendations on 
 automated microtomes.  I am specifically interested in brands other than 
 Leica (I already have two).  I would appreciate any thoughts you may have.  
 Thanks.
 
 Tom McNemar, HT(ASCP)
 Histology Co-ordinator
 Licking Memorial Health Systems
 (740) 348-4163
 (740) 348-4166
 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org
 www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org
 
 
 This e-mail, including attachments, is intended for the sole use of the 
 individual and/or entity to whom it is addressed, and contains information 
 from Licking Memorial Health Systems which is confidential or privileged. If 
 you are not the intended recipient, nor authorized to receive for the 
 intended recipient, be aware that any disclosure, copying, distribution or 
 use of the contents of this e-mail and attachments is prohibited. If you have 
 received this in error, please advise the sender by reply e-mail and delete 
 the message immediately. You may also contact the LMH Process Improvement 
 Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be 
 secure or error-free as information could be intercepted, corrupted, lost, 
 destroyed, arrive late or incomplete, or contain viruses. The sender 
 therefore does not accept liability for any errors or omissions in the 
 contents of this message, which arise as a result of e-mail transmission. 
 Thank you.
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Re: [Histonet] Microtome advise...

[Jack Ratliff]

BTW. I'm certain that the Sakura unit will be on display at the NSH S/C meeting 
in Providence, RI (20-25 Sept 2013) and I know for a fact that the laser 
microtome will be on display there as well because it will be demonstrated and 
used, along with a Leica rotary microtome for part of a workshop I am 
co-presenting with Bob Skinner (WS # 61 - A Detailed Examination of Working 
With Decalcified and Undecalcified Bone In Support of Preclinical and Clinical 
Research).

Jack


On Jul 18, 2013, at 8:55 AM, Jack Ratliff ratliffj...@hotmail.com wrote:

 How about the new Sakura with the auto orientation feature! I saw it on 
 display at the Missouri Society for Histotechnology meeting and it was quite 
 impressive! Of course if you have the budget, there's alway the non-contact 
 laser microtome from Rowiak! I have seen and used that unit to cut fresh soft 
 tissues (brain) and resin embedded bone and biomaterial implants!
 
 Jack
 
 
 On Jul 18, 2013, at 7:57 AM, Tom McNemar tmcne...@lmhealth.org wrote:
 
 Hello all.  I am preparing my budget and am seeking recommendations on 
 automated microtomes.  I am specifically interested in brands other than 
 Leica (I already have two).  I would appreciate any thoughts you may have.  
 Thanks.
 
 Tom McNemar, HT(ASCP)
 Histology Co-ordinator
 Licking Memorial Health Systems
 (740) 348-4163
 (740) 348-4166
 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org
 www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org
 
 
 This e-mail, including attachments, is intended for the sole use of the 
 individual and/or entity to whom it is addressed, and contains information 
 from Licking Memorial Health Systems which is confidential or privileged. If 
 you are not the intended recipient, nor authorized to receive for the 
 intended recipient, be aware that any disclosure, copying, distribution or 
 use of the contents of this e-mail and attachments is prohibited. If you 
 have received this in error, please advise the sender by reply e-mail and 
 delete the message immediately. You may also contact the LMH Process 
 Improvement Center at 740-348-4641. E-mail transmissions cannot be 
 guaranteed to be secure or error-free as information could be intercepted, 
 corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. 
 The sender therefore does not accept liability for any errors or omissions 
 in the contents of this message, which arise as a result of e-mail 
 transmission. Thank you.
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Re: [Histonet] microtome aligner

[Grantham, Andrea L - (algranth)]

They do work. I saw one at NSH some years ago and came home and had the guys in 
our shop make one for me exactly like the one I saw and it cost next to 
nothing. We bought the little round level at home depot.

Andi




Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edumailto:algra...@email.arizona.edu
Tel: 520.626.4415 Fax: 520.626.2097





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RE: [Histonet] microtome aligner

[Goins, Tresa]

Yikes.  There is a lot of play getting that bubble in the middle of the 
universal aligner that may translate to large variations in alignment each 
time the thing is used.  The high cost is probably for the clamp, but if the 
bubble level is worth $1.50 the quality of the clamp doesn't matter.

Save your money and use a blank paraffin block.



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maria Mejia
Sent: Wednesday, July 17, 2013 8:12 AM
To: HistoNet
Subject: [Histonet] microtome aligner

Hello,

Can anyone provide any additional information regarding microtome aligners?
Do they work?  I have never used or seen this particular tool, so which brand 
works best?  I know of only one company that sells a microtome aligner 
(universal type).
Any information any one can provide will be most appreciated.

Best
Maria Mejia
San Francisco, CA


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Re: [Histonet] microtome aligner

[Paula Sicurello]

I used an even cheaper version of the aligner.  My microtome service
engineer suggested an L level or a T level that you can buy at Home
Depot.  The L looks just like an L, it is actually a right angle measuring
device, used to make sure the corners or something are straight at 90
degrees.

You take the knife holder out and place one side of the L on the knife
holder place and the other against the block face.

Paula  :-)

-- 
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory
Duke University Health System
Rm.#251M, Duke South, Green Zone
Durham, North Carolina 27710
P:  919.684.2091

HIPAA Privacy Notification: This message and any accompanying documents are
covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521,
and contain information intended for the specific individual (s) only. This
information is confidential. If you are not the intended recipient or an
agent responsible for delivering it to the intended recipient, you are
hereby notified that you have received this document in error and that any
review, dissemination, copying or the taking of any action based on the
contents of this information is strictly prohibited . If you have received
this communication in error, please notify us immediately by e-mail, and
delete the original message.

On Wed, Jul 17, 2013 at 11:14 AM, Grantham, Andrea L - (algranth) 
algra...@email.arizona.edu wrote:

 They do work. I saw one at NSH some years ago and came home and had the
 guys in our shop make one for me exactly like the one I saw and it cost
 next to nothing. We bought the little round level at home depot.

 Andi




 Andrea Grantham, HT (ASCP)
 Senior Research Specialist
 University of Arizona
 Cellular and Molecular Medicine
 Histology Service Laboratory
 P.O.Box 245044
 Tucson, AZ 85724

 algra...@email.arizona.edumailto:algra...@email.arizona.edu
 Tel: 520.626.4415 Fax: 520.626.2097





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RE: [Histonet] microtome aligner

[joelle weaver]

Yes you can certainly align the old fashioned way easy enough, but I find these 
are very handy and do save some time. 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
 From: algra...@email.arizona.edu
 CC: histonet@lists.utsouthwestern.edu
 Date: Wed, 17 Jul 2013 15:14:16 +
 Subject: Re: [Histonet] microtome aligner
 
 They do work. I saw one at NSH some years ago and came home and had the guys 
 in our shop make one for me exactly like the one I saw and it cost next to 
 nothing. We bought the little round level at home depot.
 
 Andi
 
 
 
 
 Andrea Grantham, HT (ASCP)
 Senior Research Specialist
 University of Arizona
 Cellular and Molecular Medicine
 Histology Service Laboratory
 P.O.Box 245044
 Tucson, AZ 85724
 
 algra...@email.arizona.edumailto:algra...@email.arizona.edu
 Tel: 520.626.4415 Fax: 520.626.2097
 
 
 
 
 
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Re: [Histonet] Microtome upgrade, planning stages

[Maxim Peshkov]

I tried some different microtomes
(Leica, Microm, Thermo Shandon,
Sakura), as sledge as rotary types.
My choise is Leica RM2245.
Leica RM2245 is semi automatic? and
RM2255 or RM2265 are full automatic
models.
I like RM2245 because this model
have a rock mode, when operator
does for a section only 1/4 rotation.
It is very ergonomic!

Shandon Finesse ME also have rock
mode, but I have not happy with it.

Leica RM series have only one
disadvantage - two different type for
disposable blade holder (for low and high
profile, but not universal). Buy both
type holders.
RM2245 cheaper than 55 or 65.
Series 2125 is the same as Microm 325.

I do not try Leica Cool Clamp.

Hope this help.

Maxim Peshkov
Russia,
Taganrog.


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Re: [Histonet] Microtome upgrade, planning stages...

[Rene J Buesa]

Add another Leica to your list
René J,

From: kgrob...@rci.rutgers.edu kgrob...@rci.rutgers.edu
To: histonet histonet@lists.utsouthwestern.edu 
Sent: Thursday, March 7, 2013 2:32 PM
Subject: [Histonet] Microtome upgrade, planning stages...

We are starting to look into upgrading to higher-end, but used,
microtomes.  What we have is still working, but the writing is on the wall
for them in terms of repair/parts.

Which brands/models are considered higher-end in the used market these
days?  (Leica is already on my list, but which models?)

Thanks so much!

Kathleen


Principal Lab Technician
Neurotoxicology Labs
Molecular Pathology Facility Core
Dept of Pharmacology  Toxicology
Rutgers, the State University of NJ
41 B Gordon Road
Piscataway, NJ 08854
(848) 445-1443
FAX (732) 445-6905

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Re: [Histonet] Microtome blades

[Rene J Buesa]

Sakura
René J.

From: Laurie Colbert lcolb...@pathmdlabs.com
To: Histonet Post (histonet@lists.utsouthwestern.edu) 
histonet@lists.utsouthwestern.edu 
Sent: Wednesday, January 9, 2013 10:55 AM
Subject: [Histonet] Microtome blades

Does anyone know who I can purchase Accu-edge blades from, other than Cardinal 
or American Master Tech?

Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA  90048
(323) 648-3214 direct
(424) 245-7284 main lab

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RE: [Histonet] Microtome blades

[Bartlett, Jeanine (CDC/OID/NCEZID)]

If you order them from Cardinal they get them from SakuraI think. Or maybe 
vice-versa...

Jeanine H. Bartlett
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
404-639-3590
jeanine.bartl...@cdc.hhs.gov

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, January 09, 2013 12:15 PM
To: Laurie Colbert; Histonet Post (histonet@lists.utsouthwestern.edu)
Subject: Re: [Histonet] Microtome blades

Sakura
René J.

From: Laurie Colbert lcolb...@pathmdlabs.com
To: Histonet Post (histonet@lists.utsouthwestern.edu) 
histonet@lists.utsouthwestern.edu 
Sent: Wednesday, January 9, 2013 10:55 AM
Subject: [Histonet] Microtome blades

Does anyone know who I can purchase Accu-edge blades from, other than Cardinal 
or American Master Tech?

Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA  90048
(323) 648-3214 direct
(424) 245-7284 main lab

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Re: [Histonet] Microtome maintenance frequency

[Rene J Buesa]

For your usage level  it is unnecessary an annual maintenance. As a matter of 
fact I am sure you will be able to use those Leica microtomes for many years ar 
the same usage level without needing any capital maintenance.

René J.

From: Allen Keeping D. allen.keep...@albertahealthservices.ca
To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu 
Sent: Thursday, December 27, 2012 1:40 PM
Subject: [Histonet] Microtome maintenance frequency

I have a question regarding scheduled maintenance of rotary microtomes. I run a 
simulation lab for students of lab technology. Part of their histology rotation 
involves learning how to produce acceptable sections on the microtome. I 
currently have 3 Leica rotary (manual) microtomes, which are used by students a 
total of 18-20 days per year (for educational purposes, not to produce 
diagnostic materials). In addition, I may use a microtome for a couple weeks 
total each year to produce control materials and to troubleshoot staining 
issues.

Currently, the microtomes are serviced by a Leica-educated professional 
annually for preventative maintenance. As these microtomes are only used 
infrequently, is this level of maintenance necessary (or recommended)? I am 
contemplating switching to a bi-annual schedule as a cost-saving measure.

Does anyone have any advice or experience regarding maintaining microtomes in a 
low-volume setting?

Cheers,

Allen



  
This message and any attached documents are only for the use of the intended 
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unauthorized review, use, retransmission, or other disclosure is strictly 
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RE: [Histonet] Microtome knives

[Patsy Ruegg]

There are knife sharpening services Sturkey and Dorn and Hart are two that
come to mind.  You can also find refurbished disposable blade holders and
buy disposable blades, the blade holder you get will determine if you use
low profile or high profile blades.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax: 720-859-4110
pru...@ihctech.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Massimo
Sent: Sunday, November 11, 2012 2:45 AM
To: Jon Krupp; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome knives

I prefer to sharpen my microtome knives by myself by hand.
I have a vintage Cambridge Rocking Microtome and despite its age it works
very well.
Sharpening is a time consuming for the first time, it's depends on the
conditions of the blade edge.
Once you have a nice cutting profile its maintenance it's quite easy and it
takes a few minutes by
stroking the knife on a flat glass with oil and a bit of aluminium oxide
powder (3 -1 micron grits).
For me sharpening and honing of a microtome knife has became a secondary
hobby.
A solid knife has the advantage, compared to a disposable blade, to be
liable to less vibrations.

Kind Regards,
Massimo Tosi


A humble Chemical
Engineer who loves Histology






 Da: Jon Krupp jkr...@deltacollege.edu
A: Histonet@lists.utsouthwestern.edu 
Inviato: Venerdì 9 Novembre 2012 19:49
Oggetto: [Histonet] Microtome knives
 
Greetings

I need some advice regarding microtome knives. I am not  histo tech, I did
all my sectioning in a plant research lab, but now I find myself needing to
learn more about histo type methods.

We have microtomes, AO 820's, and we have a bunch of donated knives. I need
advice about whether it would be better to find a knife sharpener and use
the microtome knives we have, or check into getting a disposable knife
holder. 

When I was sectioning, we just used a simple razor blade holder. Now I see
references to high profile and low profile blades and holders, and I don't
know the difference. 

Anyone willing to help me out?

Thanks

Jon

Jonathan Krupp
Delta College
5151 Pacific Ave.
Box 212
Stockton, CA  95207
209-954-5284
jkr...@deltacollege.edu

Find us on Facebook @
Electron Microscopy at SJ Delta College







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Re: [Histonet] Microtome knives

[Massimo]

I prefer to sharpen my microtome knives by myself by hand.
I have a vintage Cambridge Rocking Microtome and despite its age it works very 
well.
Sharpening is a time consuming for the first time, it's depends on the 
conditions of the blade edge.
Once you have a nice cutting profile its maintenance it's quite easy and it 
takes a few minutes by
stroking the knife on a flat glass with oil and a bit of aluminium oxide powder 
(3 -1 micron grits).
For me sharpening and honing of a microtome knife has became a secondary 
hobby.
A solid knife has the advantage, compared to a disposable blade, to be liable 
to less vibrations.

Kind Regards,
Massimo Tosi


A humble Chemical
Engineer who loves Histology






 Da: Jon Krupp jkr...@deltacollege.edu
A: Histonet@lists.utsouthwestern.edu 
Inviato: Venerdì 9 Novembre 2012 19:49
Oggetto: [Histonet] Microtome knives
 
Greetings

I need some advice regarding microtome knives. I am not  histo tech, I did all 
my sectioning in a plant research lab, but now I find myself needing to learn 
more about histo type methods.

We have microtomes, AO 820's, and we have a bunch of donated knives. I need 
advice about whether it would be better to find a knife sharpener and use the 
microtome knives we have, or check into getting a disposable knife holder. 

When I was sectioning, we just used a simple razor blade holder. Now I see 
references to high profile and low profile blades and holders, and I don't know 
the difference. 

Anyone willing to help me out?

Thanks

Jon

Jonathan Krupp
Delta College
5151 Pacific Ave.
Box 212
Stockton, CA  95207
209-954-5284
jkr...@deltacollege.edu

Find us on Facebook @
Electron Microscopy at SJ Delta College







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RE: [Histonet] Microtome knives

[HARRISON,Sharon R]

Hi All,
I am Sharon Harrison, supervisor of Histopathology lab at UWI Mona in Kingston 
Jamaica.

I agree with you that we still need to have the solid blades for the cutting of 
hard tissue such as cervix fibroids and bone. Hence we have to maintain some 
vestige of the old technology of knife sharpening, as the disposable blades are 
quite expensive.

The disposable blades are great for biopsies and softer tissue but a real pain 
for getting sections of the harder tissues.

Therefore I advice having both systems especially if your laboratory handles 
all types of tissue specimen.
Sharon Harrison
Chief Medical Technologist
DMT, BSc, MPH




From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Massimo 
[max_histo...@yahoo.it]
Sent: Sunday, November 11, 2012 4:44 AM
To: Jon Krupp; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome knives

I prefer to sharpen my microtome knives by myself by hand.
I have a vintage Cambridge Rocking Microtome and despite its age it works very 
well.
Sharpening is a time consuming for the first time, it's depends on the 
conditions of the blade edge.
Once you have a nice cutting profile its maintenance it's quite easy and it 
takes a few minutes by
stroking the knife on a flat glass with oil and a bit of aluminium oxide powder 
(3 -1 micron grits).
For me sharpening and honing of a microtome knife has became a secondary 
hobby.
A solid knife has the advantage, compared to a disposable blade, to be liable 
to less vibrations.

Kind Regards,
Massimo Tosi


A humble Chemical
Engineer who loves Histology






 Da: Jon Krupp jkr...@deltacollege.edu
A: Histonet@lists.utsouthwestern.edu
Inviato: Venerdì 9 Novembre 2012 19:49
Oggetto: [Histonet] Microtome knives

Greetings

I need some advice regarding microtome knives. I am not  histo tech, I did all 
my sectioning in a plant research lab, but now I find myself needing to learn 
more about histo type methods.

We have microtomes, AO 820's, and we have a bunch of donated knives. I need 
advice about whether it would be better to find a knife sharpener and use the 
microtome knives we have, or check into getting a disposable knife holder.

When I was sectioning, we just used a simple razor blade holder. Now I see 
references to high profile and low profile blades and holders, and I don't know 
the difference.

Anyone willing to help me out?

Thanks

Jon

Jonathan Krupp
Delta College
5151 Pacific Ave.
Box 212
Stockton, CA  95207
209-954-5284
jkr...@deltacollege.edu

Find us on Facebook @
Electron Microscopy at SJ Delta College







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Re: [Histonet] Microtome knives

[Rene J Buesa]

Because of your donated knives  you will have to buy a knives sharpener which 
are costly and not very easy to find.
Your best option is to buy a high profile disposable blades holder (that will 
be cheaper), buy disposable blades and avoid all the frustrations and waste of 
time sharpening knives.
René J.




From: Jon Krupp jkr...@deltacollege.edu
To: Histonet@lists.utsouthwestern.edu 
Sent: Friday, November 9, 2012 1:49 PM
Subject: [Histonet] Microtome knives

Greetings

I need some advice regarding microtome knives. I am not  histo tech, I did all 
my sectioning in a plant research lab, but now I find myself needing to learn 
more about histo type methods.

We have microtomes, AO 820's, and we have a bunch of donated knives. I need 
advice about whether it would be better to find a knife sharpener and use the 
microtome knives we have, or check into getting a disposable knife holder. 

When I was sectioning, we just used a simple razor blade holder. Now I see 
references to high profile and low profile blades and holders, and I don't know 
the difference. 

Anyone willing to help me out?

Thanks

Jon

Jonathan Krupp
Delta College
5151 Pacific Ave.
Box 212
Stockton, CA  95207
209-954-5284
jkr...@deltacollege.edu

Find us on Facebook @
Electron Microscopy at SJ Delta College







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RE: [Histonet] Microtome knives

[Connolly, Brett M]

Hi Jon,

Depends on your cash flow. You could get a used sharpener somewhere off the web 
somewhere such as 
http://www.labx.com/v2/adsearch/resultsnew.cfm?sw=sharpenermr=25te=cat , or 
http://www.medwow.com/used-microtome-knife-sharpener-equipment/63.med but 
sharpening knives is a pain IMO and steel knives present more of a safety 
hazard. I would recommend a sharpener that uses the glass honing plates. You 
would also need the coarse and fine abrasives.

Personally, I would opt for a low profile disposable blade holder that fits 
your 820. Low and high profile refer to the size (height) of the blade. We use 
low profile for paraffin block sectioning and high profile for cryostat 
sectioning.

Brett

Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jon Krupp
Sent: Friday, November 09, 2012 1:50 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtome knives

Greetings

I need some advice regarding microtome knives. I am not  histo tech, I did all 
my sectioning in a plant research lab, but now I find myself needing to learn 
more about histo type methods.

We have microtomes, AO 820's, and we have a bunch of donated knives. I need 
advice about whether it would be better to find a knife sharpener and use the 
microtome knives we have, or check into getting a disposable knife holder. 

When I was sectioning, we just used a simple razor blade holder. Now I see 
references to high profile and low profile blades and holders, and I don't know 
the difference. 

Anyone willing to help me out?

Thanks

Jon

Jonathan Krupp
Delta College
5151 Pacific Ave.
Box 212
Stockton, CA  95207
209-954-5284
jkr...@deltacollege.edu

Find us on Facebook @
Electron Microscopy at SJ Delta College







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for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
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Re: [Histonet] microtome

[E. Wayne Johnson]

I got it open and cleaned the wheel stop mechanism and
the belt in front and it works good now and we now have a backup and
my people are smiling.

Thanks for everyone's helpful advices.

Wayne Johnson
Enruikang (Enable) Ag Tech
Beijing

On 9/22/2012 4:34 AM, Burton, Lynn wrote:

Perhaps they have a contact person since they do so much work throughout the US.

From: jsjurc...@comcast.net [mailto:jsjurc...@comcast.net]
Sent: Friday, September 21, 2012 3:16 PM
To: Jay Lundgren
Cc: Histonet; Burton, Lynn
Subject: Re: [Histonet] microtome

As I said, I'm not sure they have anybody in Beijing.

From: Jay Lundgrenjaylundg...@gmail.com
To: Lynn Burtonlynn.bur...@illinois.gov
Cc: Histonethistonet@lists.utsouthwestern.edu
Sent: Friday, September 21, 2012 2:27:59 PM
Subject: Re: [Histonet] microtome

Um.he is in Beijing.
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Re: [Histonet] microtome

[E. Wayne Johnson]

I think its a Leica 2125.

On 9/22/2012 12:32 AM, E. Wayne Johnson wrote:

We work in a previously underutilized histology lab.

There was a sliding horizontal Sakura microtome, a functioning Thermo 
microtome,

and a nonfunctioning Leica 5xxx (not a very fancy Leica).

The center where we work has no interest in fixing the Leica microtome.
I am supposing that it is merely dirty.  Beijing is very dusty and the 
windows to

the room leaked badly until we installed window seals.

I would like to try to take the Leica microtome apart and try to clean 
it but I cant find
the way to get it open.  There is no hatch to open like on an old AO 
Spencer.


We are told that it would cost way to much to have Leica service it.

Does anyone have any experience with maintenance or repair of a 
low-end Leica Microtome

and have any tips to share on how to open and clean it?

Wayne Johnson
Beijing.

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RE: [Histonet] microtome

[Burton, Lynn]

I would try Tech One Biomedical. They have a great staff and they have done all 
of our repairs for a long time.
1-866-497-3033 www.techoneweb.com

Lynn Burton
Galesburg Animal Disease Lab
Galesburg, Il

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of E. Wayne Johnson
Sent: Friday, September 21, 2012 11:33 AM
To: Histonet
Subject: [Histonet] microtome

We work in a previously underutilized histology lab.

There was a sliding horizontal Sakura microtome, a functioning Thermo 
microtome,
and a nonfunctioning Leica 5xxx (not a very fancy Leica).

The center where we work has no interest in fixing the Leica microtome.
I am supposing that it is merely dirty.  Beijing is very dusty and the 
windows to
the room leaked badly until we installed window seals.

I would like to try to take the Leica microtome apart and try to clean 
it but I cant find
the way to get it open.  There is no hatch to open like on an old AO 
Spencer.

We are told that it would cost way to much to have Leica service it.

Does anyone have any experience with maintenance or repair of a low-end 
Leica Microtome
and have any tips to share on how to open and clean it?

Wayne Johnson
Beijing.

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Re: [Histonet] microtome

[Jay Lundgren]

Um.he is in Beijing.
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Re: [Histonet] microtome

[jsjurczak]

As I said, I'm not sure they have anybody in Beijing. 

- Original Message -
From: Jay Lundgren jaylundg...@gmail.com 
To: Lynn Burton lynn.bur...@illinois.gov 
Cc: Histonet histonet@lists.utsouthwestern.edu 
Sent: Friday, September 21, 2012 2:27:59 PM 
Subject: Re: [Histonet] microtome 

Um.he is in Beijing. 
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RE: [Histonet] microtome

[Burton, Lynn]

Perhaps they have a contact person since they do so much work throughout the US.

From: jsjurc...@comcast.net [mailto:jsjurc...@comcast.net]
Sent: Friday, September 21, 2012 3:16 PM
To: Jay Lundgren
Cc: Histonet; Burton, Lynn
Subject: Re: [Histonet] microtome

As I said, I'm not sure they have anybody in Beijing.

From: Jay Lundgren jaylundg...@gmail.com
To: Lynn Burton lynn.bur...@illinois.gov
Cc: Histonet histonet@lists.utsouthwestern.edu
Sent: Friday, September 21, 2012 2:27:59 PM
Subject: Re: [Histonet] microtome

Um.he is in Beijing.
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Re: [Histonet] microtome

[E. Wayne Johnson]

Tech One seems to be limited in scope to the US.

Nice hearing from you in Galesburg. I practiced pig production medicine 
in Illinois for quite a few years.

Doug Hoefling the former director and pathologist in Galesburg
is an old friend and mentor of mine, but of course he is long since retired.

Histopathology is not yet a well-developed tool for pig disease 
diagnosis in China.
We are presently one of the few diagnostic centers in China, perhaps the 
only one that routinely

does histopath on pig necropsy cases.

On 9/22/2012 4:34 AM, Burton, Lynn wrote:

Perhaps they have a contact person since they do so much work throughout the US.

From: jsjurc...@comcast.net [mailto:jsjurc...@comcast.net]
Sent: Friday, September 21, 2012 3:16 PM
To: Jay Lundgren
Cc: Histonet; Burton, Lynn
Subject: Re: [Histonet] microtome

As I said, I'm not sure they have anybody in Beijing.

From: Jay Lundgrenjaylundg...@gmail.com
To: Lynn Burtonlynn.bur...@illinois.gov
Cc: Histonethistonet@lists.utsouthwestern.edu
Sent: Friday, September 21, 2012 2:27:59 PM
Subject: Re: [Histonet] microtome

Um.he is in Beijing.
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RE: [Histonet] Microtome feedback

[Bea DeBrosse-Serra]

It is a high quality work horse. I've worked with newer and older microtomes of 
this very model and never had any problems. You can utilize the motorized 
function to minimize repetitive motion, but you can also use it manually if 
needed. What is there not to like? 

Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371



-Original Message-
From: Ian R Bernard [mailto:ibern...@uab.edu] 
Sent: Saturday, August 18, 2012 3:53 AM
To: Bea DeBrosse-Serra; Fred Underwood; Histonet
Subject: RE: [Histonet] Microtome feedback

What is great about the Leica RM2255?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bea 
DeBrosse-Serra
Sent: Friday, August 17, 2012 8:58 PM
To: Fred Underwood; Histonet
Subject: RE: [Histonet] Microtome feedback

Fred,

The Leica RM2255 all the way!

Go Dayton!

Bea

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fred Underwood 
[funderw...@mcohio.org]
Sent: Friday, August 17, 2012 12:48 PM
To: Histonet
Subject: [Histonet] Microtome feedback

Hello and TGIF to everyone.  I'm exploring getting a new microtome and would 
like to draw on the vast knowledge and experience out there in Histoland.  The 
three I'm looking at are:  the Zeiss Hyrax M55, Thermo Scientific ME+, and the 
Leica RM2255.

Thanks,
Fred Underwood
Montgomery County Coroner's Office
Dayton, OH

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RE: [Histonet] Microtome feedback

[Lynette Pavelich]

We purchased the Leica RM2255 just over a year ago. It is the only one we have 
and is primarily used by one tech. In her absence, others use her machine. What 
is great about it, is that it is VERY easy to adapt to it. The controls are 
easy to figure out, cuts beautiful slides, and can be used either manually or 
completely automatic. Additionally, it has a homing option for the block 
holder.pretty handy if you need to adjust the block holder often!
In the future, I plan on replacing 2 more microtomes with this Leica microtome.

Lynette

Lynette Pavelich, HT(ASCP)
Histology Supervisor
Hurley Medical Center
One Hurley Plaza
Flint, MI 48503

ph: 810.262.9948
mobile: 810.444.7966


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Fred Underwood 
[funderw...@mcohio.org]
Sent: Friday, August 17, 2012 3:48 PM
To: Histonet
Subject: [Histonet] Microtome feedback

Hello and TGIF to everyone.  I'm exploring getting a new microtome and would 
like to draw on the vast knowledge and experience out there in Histoland.  The 
three I'm looking at are:  the Zeiss Hyrax M55, Thermo Scientific ME+, and the 
Leica RM2255.

Thanks,
Fred Underwood
Montgomery County Coroner's Office
Dayton, OH
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RE: [Histonet] Microtome feedback

[Ian R Bernard]

What is great about the Leica RM2255?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bea 
DeBrosse-Serra
Sent: Friday, August 17, 2012 8:58 PM
To: Fred Underwood; Histonet
Subject: RE: [Histonet] Microtome feedback

Fred,

The Leica RM2255 all the way!

Go Dayton!

Bea

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fred Underwood 
[funderw...@mcohio.org]
Sent: Friday, August 17, 2012 12:48 PM
To: Histonet
Subject: [Histonet] Microtome feedback

Hello and TGIF to everyone.  I'm exploring getting a new microtome and would 
like to draw on the vast knowledge and experience out there in Histoland.  The 
three I'm looking at are:  the Zeiss Hyrax M55, Thermo Scientific ME+, and the 
Leica RM2255.

Thanks,
Fred Underwood
Montgomery County Coroner's Office
Dayton, OH

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Re: [Histonet] Microtome feedback

[Kim Tournear]

Leica

Sent from the iPhone of Kim Tournear  

On Aug 17, 2012, at 2:48 PM, Fred Underwood funderw...@mcohio.org wrote:

 Hello and TGIF to everyone.  I'm exploring getting a new microtome and would 
 like to draw on the vast knowledge and experience out there in Histoland.  
 The three I'm looking at are:  the Zeiss Hyrax M55, Thermo Scientific ME+, 
 and the Leica RM2255.
 
 Thanks,
 Fred Underwood
 Montgomery County Coroner's Office
 Dayton, OH
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RE: [Histonet] Microtome feedback

[Marcum, Pamela A]

The RM2255 is a workhorse.  We have three of those and 3 RM2155s as an older 
model.   The 2255 is the best.  I had used the 2255 in the past to cut plastic 
with a tungsten carbide knife and it held up better than anything else 
available at the time.

Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fred Underwood
Sent: Friday, August 17, 2012 2:49 PM
To: Histonet
Subject: [Histonet] Microtome feedback

Hello and TGIF to everyone.  I'm exploring getting a new microtome and would 
like to draw on the vast knowledge and experience out there in Histoland.  The 
three I'm looking at are:  the Zeiss Hyrax M55, Thermo Scientific ME+, and the 
Leica RM2255.
 
Thanks,
Fred Underwood
Montgomery County Coroner's Office
Dayton, OH
Confidentiality Notice: This e-mail message, including any attachments,
is for the sole use of the intended recipient(s) and may contain
confidential and privileged information.  Any unauthorized review,
use, disclosure or distribution is prohibited.  If you are not the 
intended recipient, please contact the sender by reply
e-mail and destroy all copies of the original message.


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RE: [Histonet] Microtome feedback

[Starbuck,Michael W]

Leica RM2255 for plastic and paraffin - no problems

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fred Underwood
Sent: Friday, August 17, 2012 2:49 PM
To: Histonet
Subject: [Histonet] Microtome feedback

Hello and TGIF to everyone.  I'm exploring getting a new microtome and would 
like to draw on the vast knowledge and experience out there in Histoland.  The 
three I'm looking at are:  the Zeiss Hyrax M55, Thermo Scientific ME+, and the 
Leica RM2255.
 
Thanks,
Fred Underwood
Montgomery County Coroner's Office
Dayton, OH

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RE: [Histonet] Microtome feedback

[Bea DeBrosse-Serra]

Fred,

The Leica RM2255 all the way!

Go Dayton!

Bea

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fred Underwood 
[funderw...@mcohio.org]
Sent: Friday, August 17, 2012 12:48 PM
To: Histonet
Subject: [Histonet] Microtome feedback

Hello and TGIF to everyone.  I'm exploring getting a new microtome and would 
like to draw on the vast knowledge and experience out there in Histoland.  The 
three I'm looking at are:  the Zeiss Hyrax M55, Thermo Scientific ME+, and the 
Leica RM2255.

Thanks,
Fred Underwood
Montgomery County Coroner's Office
Dayton, OH

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RE: [Histonet] microtome

[Stephenson, Sheryl]

If you want to stay with Leica;  they have a newer model called RM2255 it has 
the manual and automatic rotations.

Sheryl Stephenson | Histology Technician 

   


 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Véronique Barrès
Sent: Friday, June 08, 2012 10:14 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] microtome

Hi histonetters!

We are a molecular biology lab with a histology facility (we do whole FFPE
tissue sections to build TMAs and then TMA sectionning). We currently have
a Leica RM2125 in the lab, but want to acquire a new manual microtome,
mainly as a safe because we depend a lot on tissue sectioning. Could anyone
give me some advice/suggestion on which microtome to buy?
We only use the regular cassettes.

Best regards,
Véronique
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RE: [Histonet] microtome

[Bea DeBrosse-Serra]

Leica 2255, all the way.

Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Véronique Barrès
Sent: Friday, June 08, 2012 7:14 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] microtome

Hi histonetters!

We are a molecular biology lab with a histology facility (we do whole FFPE 
tissue sections to build TMAs and then TMA sectionning). We currently have a 
Leica RM2125 in the lab, but want to acquire a new manual microtome, mainly as 
a safe because we depend a lot on tissue sectioning. Could anyone give me some 
advice/suggestion on which microtome to buy?
We only use the regular cassettes.

Best regards,
Véronique
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Re: [Histonet] microtome service/repair Denver area

[abright]

Dear Liz,

Why don't you use Leica services?

Alan Bright 
Sent from my BlackBerry® wireless device

-Original Message-
From: Elizabeth Chlipala l...@premierlab.com
Sender: histonet-boun...@lists.utsouthwestern.edu
Date: Wed, 3 Aug 2011 15:55:39 
To: histonet@lists.utsouthwestern.eduhistonet@lists.utsouthwestern.edu
Subject: [Histonet] microtome service/repair Denver area

Hello everyone

I was wondering if anyone has any recommendations for a vendor that does yearly 
service and repair on Leica microtomes and cryostats.  We have been working 
with one vendor for years now and always have been happy with their service but 
they have become very expensive, any suggestions would be appreciated.

Thanks in advance

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.comhttp://www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

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RE: [Histonet] microtome service/repair Denver area

[Weems, Joyce]

She said they've become too expensive. We all must economize these days!  


Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
abri...@brightinstruments.com
Sent: Thursday, August 04, 2011 08:39
To: Elizabeth Chlipala; histonet-boun...@lists.utsouthwestern.edu; Histonet
Subject: Re: [Histonet] microtome service/repair Denver area

Dear Liz,

Why don't you use Leica services?

Alan Bright
Sent from my BlackBerry(r) wireless device

-Original Message-
From: Elizabeth Chlipala l...@premierlab.com
Sender: histonet-boun...@lists.utsouthwestern.edu
Date: Wed, 3 Aug 2011 15:55:39
To: histonet@lists.utsouthwestern.eduhistonet@lists.utsouthwestern.edu
Subject: [Histonet] microtome service/repair Denver area

Hello everyone

I was wondering if anyone has any recommendations for a vendor that does yearly 
service and repair on Leica microtomes and cryostats.  We have been working 
with one vendor for years now and always have been happy with their service but 
they have become very expensive, any suggestions would be appreciated.

Thanks in advance

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 
18592 Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.comhttp://www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

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This e-mail, including any attachments is the 
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Re: [Histonet] Microtome Repair

[DKBoyd]

Tech One Biomedical  866-497-3033

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkb...@chs.net







Wimer, Helen wim...@si.edu 
Sent by: histonet-boun...@lists.utsouthwestern.edu
07/27/2011 09:42 AM

To
histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
cc

Subject
[Histonet] Microtome Repair







 Can  anyone recommend  a company that does microtome repair in the 
Washington, DC/ Maryland area?  Thanks!

Helen F Wimer HT (ASCP)
Smithsonian Institution
Department of Vertebrate Zoology
Washington, DC
(301) 496-1391
wim...@si.edu
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RE: [Histonet] Microtome Repair

[Wimer, Helen]

Thanks to all that responded to my question!

Helen F Wimer HT (ASCP)
Smithsonian Institution
Department of Vertebrate Zoology
Washington, DC
(301) 496-1391
wim...@si.edu

From: dkb...@chs.net [dkb...@chs.net]
Sent: Wednesday, July 27, 2011 11:05 AM
To: Wimer, Helen
Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome Repair

Tech One Biomedical  866-497-3033

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkb...@chs.net






Wimer, Helen wim...@si.edu
Sent by: histonet-boun...@lists.utsouthwestern.edu

07/27/2011 09:42 AM


To
histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
cc

Subject
[Histonet] Microtome Repair








Can  anyone recommend  a company that does microtome repair in the Washington, 
DC/ Maryland area?  Thanks!

Helen F Wimer HT (ASCP)
Smithsonian Institution
Department of Vertebrate Zoology
Washington, DC
(301) 496-1391
wim...@si.edu
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RE: [Histonet] Microtome draft shield

[Gomez, Milton]

negative pressure will make the ribbons have a flying party.  is it feassible 
to built a room within the lab with no pressure for sectioning?

mg


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni 
[trathbo...@somerset-healthcare.com]
Sent: Tuesday, July 19, 2011 9:11 AM
To: 'Keri Colwell'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Microtome draft shield

Haven't seen anything that specific, but you might want to look at the various 
biohazard splash guards. They are a clear Plexiglas, and they have a base to 
support them. You would be able to move them around (or have them mounted to 
the counter if desired), and they come in an assortment of angles and sizes.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Keri Colwell
Sent: Monday, July 18, 2011 5:21 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtome draft shield

Hi Everyone,

I work in a lab which is under negative pressure (air is continuously flowing 
in), and due to the layout of the rooms are microtomes are located next to two 
different doorways.  We are looking for some sort of draft shield to place 
around each microtome and water bath that will reduce the effects of the 
airflow and personnel movements on our ribbons.

Anyone have any suggestions as to who might sell such a thing?

Thanks in advance!




Keri Colwell
Laboratory Technologist | Technologiste de laboratoire TSE and Pathology 
Lethbridge Laboratory | Laboratoire de Lethbridge Canadian Food Inspection 
Agency | Agence candienne d'inspection des aliments Township Road 9-1 | Ch de 
Canton 9-1 Box 640  | CP 640 Lethbridge, AB T1J 3Z4 E-mail | Courriel: 
keri.colw...@inspection.gc.ca Telephone | Téléphone:  403-382-5500 Facsimile | 
Télécopieur: 403-382-5583 Government of Canada | Gouvernement du Canada

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Re: [Histonet] Microtome draft shield

[abright]

Dear Keri,

Our  Bright M3500 microtome is fitted with a clear retractable visor over the 
specimen and knife area that will more than likely solve your problem. Please 
take a look on www.brightinstruments.com

Alan Bright
Bright Instrument Co.
Cambs
England 
Sent from my BlackBerry® wireless device

-Original Message-
From: Keri Colwell keri.colw...@inspection.gc.ca
Sender: histonet-boun...@lists.utsouthwestern.edu
Date: Mon, 18 Jul 2011 17:21:05 
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtome draft shield

Hi Everyone,

I work in a lab which is under negative pressure (air is continuously
flowing in), and due to the layout of the rooms are microtomes are
located next to two different doorways.  We are looking for some sort of
draft shield to place around each microtome and water bath that will
reduce the effects of the airflow and personnel movements on our
ribbons.

Anyone have any suggestions as to who might sell such a thing?

Thanks in advance!




Keri Colwell
Laboratory Technologist | Technologiste de laboratoire
TSE and Pathology
Lethbridge Laboratory | Laboratoire de Lethbridge
Canadian Food Inspection Agency | Agence candienne d'inspection des
aliments
Township Road 9-1 | Ch de Canton 9-1
Box 640  | CP 640
Lethbridge, AB T1J 3Z4
E-mail | Courriel: keri.colw...@inspection.gc.ca 
Telephone | Téléphone:  403-382-5500
Facsimile | Télécopieur: 403-382-5583
Government of Canada | Gouvernement du Canada

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RE: [Histonet] Microtome draft shield

[Rathborne, Toni]

Haven't seen anything that specific, but you might want to look at the various 
biohazard splash guards. They are a clear Plexiglas, and they have a base to 
support them. You would be able to move them around (or have them mounted to 
the counter if desired), and they come in an assortment of angles and sizes.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Keri Colwell
Sent: Monday, July 18, 2011 5:21 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtome draft shield

Hi Everyone,

I work in a lab which is under negative pressure (air is continuously flowing 
in), and due to the layout of the rooms are microtomes are located next to two 
different doorways.  We are looking for some sort of draft shield to place 
around each microtome and water bath that will reduce the effects of the 
airflow and personnel movements on our ribbons.

Anyone have any suggestions as to who might sell such a thing?

Thanks in advance!




Keri Colwell
Laboratory Technologist | Technologiste de laboratoire TSE and Pathology 
Lethbridge Laboratory | Laboratoire de Lethbridge Canadian Food Inspection 
Agency | Agence candienne d'inspection des aliments Township Road 9-1 | Ch de 
Canton 9-1 Box 640  | CP 640 Lethbridge, AB T1J 3Z4 E-mail | Courriel: 
keri.colw...@inspection.gc.ca Telephone | Téléphone:  403-382-5500 Facsimile | 
Télécopieur: 403-382-5583 Government of Canada | Gouvernement du Canada

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This message and any included attachments are from Somerset Medical Center
and are intended only for the addressee.  The information contained in this
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exemption from disclosure under applicable law.  Unauthorized forwarding,
printing, copying, distribution, or use of such information is strictly
prohibited and may be unlawful.  If you are not the addressee, please
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by e-mail or you may call Somerset Medical Center's computer Help Desk
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RE: [Histonet] microtome knife holder

[Liette Tougas]

Thank you Hugh and all others who replied.

In fact what I really meant was rather a knife clamp, I guess, as on the 
older microtomes, where we insert either a regular knife.

So my next question is: does anyone still have a knife clamp for either a 
2030 Reichert Jung microtome or a Leica microtome.  I found those pictures on 
the web that represent what I am looking for.

Thank you again in advance,

Liette

Liette Tougas, RT, B.Sc., M.Sc.
Biomedical Laboratory Technology Department
Dawson College, Montreal, Qc
514-931-8731, ext 1519
[http://images.dotmed.com/cgi-bin/size.pl?t=2a=3i=779208.jpg][http://websites.labx.com/rankin/pics/3066.JPG]

From: Hugh Luk [hlu...@msn.com]
Sent: July 12, 2011 4:04 PM
To: Liette Tougas
Subject: RE: [Histonet] microtome knife holder

Liette,

The Reichert Jung 2030 came with a choice of disposable blade (see picture) or 
fixed blade (see link) holders.  One...never both:-(

http://cgi.ebay.com/ws/eBayISAPI.dll?ViewItemitem=110710454143clk_rvr_id=247196707126

When Leica purchased Reichert, Jung and other microtome lines, they absorbed 
them into their new units.  Meaning, they do not sell parts or encourage repair 
of older units.  We have replaced all of our aging 2030's with 2255's.  And 
sorry, they were disposed of.

Rene is correct in his statement, sort of.  Technically, there is a blade 
holder that looks like a long knife for disposable blades; if you had this and 
the fixed blade holder, you would be fine.  I'm guessing Rene's lab used this 
set-up.  Everyone in Hawaii, went the other way.

Good luck with parts.  Feel free to ask histonet again.  Your best bet is to 
find someone who is getting rid of their unit, as the knife holder should have 
never worn out.

Hugh
Hawaii


 Date: Fri, 8 Jul 2011 07:03:53 -0700
 From: rjbu...@yahoo.com
 To: histonet@lists.utsouthwestern.edu; ltou...@dawsoncollege.qc.ca
 Subject: Re: [Histonet] microtome knife holder
 CC:

 Knives (not disposable blades) do not need holders. They are used by 
 themselves.
 René J.

 --- On Thu, 7/7/11, Liette Tougas ltou...@dawsoncollege.qc.ca wrote:


 From: Liette Tougas ltou...@dawsoncollege.qc.ca
 Subject: [Histonet] microtome knife holder
 To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
 Date: Thursday, July 7, 2011, 4:23 PM


 Hi again everyone,

 I also wanted to ask if anyone has, or new if there was ever, a regular knife 
 (not blade) holder for the Reichert Yung 2030 microtome and/or the Leica 
 microtome series.

 thank you again in advance,

 Liette Tougas, RT, B.Sc., M.Sc.
 Biomedical Laboratory Technology Department
 Dawson College
 514-931-8731, ext 1519
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Re: [Histonet] microtome knife holder

[Emily Sours]

All of the Leica cryostats have knife holders, I would contact Leica to ask
as their website is terrible.
You could also just google it and I bet some other company sells them.
The other one, I'm not sure of.
We don't use disposable blades so we've never had a knife holder for that.

Emily


A great book should leave you with many experiences, and slightly exhausted.
You should live several lives while reading it.
-William Styron



On Thu, Jul 7, 2011 at 4:23 PM, Liette Tougas
ltou...@dawsoncollege.qc.cawrote:

 Hi again everyone,

 I also wanted to ask if anyone has, or new if there was ever, a regular
 knife (not blade) holder for the Reichert Yung 2030 microtome and/or the
 Leica microtome series.

 thank you again in advance,

 Liette Tougas, RT, B.Sc., M.Sc.
 Biomedical Laboratory Technology Department
 Dawson College
 514-931-8731, ext 1519
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Re: [Histonet] microtome knife holder

[Rene J Buesa]

Knives (not disposable blades) do not need holders. They are used by themselves.
René J.

--- On Thu, 7/7/11, Liette Tougas ltou...@dawsoncollege.qc.ca wrote:


From: Liette Tougas ltou...@dawsoncollege.qc.ca
Subject: [Histonet] microtome knife holder
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date: Thursday, July 7, 2011, 4:23 PM


Hi again everyone,

I also wanted to ask if anyone has, or new if there was ever, a regular knife 
(not blade) holder for the Reichert Yung 2030 microtome and/or the Leica 
microtome series.

thank you again in advance,

Liette Tougas, RT, B.Sc., M.Sc.
Biomedical Laboratory Technology Department
Dawson College
514-931-8731, ext 1519
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RE: [Histonet] microtome knife holder

[Connolly, Brett M]

Um, actually steel knives do in fact require a holder and disposable blades 
require a different holder, we have both from Leica.

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, July 08, 2011 10:04 AM
To: histonet@lists.utsouthwestern.edu; Liette Tougas
Subject: Re: [Histonet] microtome knife holder

Knives (not disposable blades) do not need holders. They are used by themselves.
René J.

--- On Thu, 7/7/11, Liette Tougas ltou...@dawsoncollege.qc.ca wrote:


From: Liette Tougas ltou...@dawsoncollege.qc.ca
Subject: [Histonet] microtome knife holder
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date: Thursday, July 7, 2011, 4:23 PM


Hi again everyone,

I also wanted to ask if anyone has, or new if there was ever, a regular knife 
(not blade) holder for the Reichert Yung 2030 microtome and/or the Leica 
microtome series.

thank you again in advance,

Liette Tougas, RT, B.Sc., M.Sc.
Biomedical Laboratory Technology Department
Dawson College
514-931-8731, ext 1519
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Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
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for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
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Re: [Histonet] microtome blade donations

[Nicole Tatum]

Do they take volunteer histos, :)  I would love to do somethig like that.


Nicole




 Hi,
 One of our pathologists is going on a volunteer mission to a pathology lab
 in Malawi, Africa in a few weeks, and he is looking for donations of low
 profile microtome blades for the histology lab there.  He said they will
 need around 200 blades or so, but any amount would help.  He leaves on
 July 21st, so we are under a bit of a time crunch.  Thanks!

 Mandy M Bell , HTL (ASCP)
 Histology Department
 Community Hospital of the Monterey Peninsula
 831.625.4791


 P Please consider the environment before printing this e-mail

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Re: [Histonet] microtome safety

[Sheila Haas]

Every tech in my facility uses something different. Some use fingers, some use 
forceps (usually curved), one uses a teasing needle and one uses a paint brush. 
It's up to the individual's technique but we do try to discourage using one's 
fingers too close to the blade.
Hope this helps.
 
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
 





From: Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Tue, March 8, 2011 8:20:16 AM
Subject: [Histonet] microtome safety

Morning all!

I need some quick responses to this question:  do you use your fingers or an 
instrument of some sort to pull your paraffin ribbons off the block when 
sectioning?  For those that do not use their fingers, what do you use?  If 
forceps, are these the typical lab forceps or a special type?

Thanks so much!

Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov



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RE: [Histonet] microtome safety

[Rathborne, Toni]

Same here. One tech keeps her index fingernail extra long for this purpose.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Sheila
Haas
Sent: Tuesday, March 08, 2011 8:26 AM
To: Bartlett, Jeanine (CDC/OID/NCEZID);
histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] microtome safety


Every tech in my facility uses something different. Some use fingers, some use 
forceps (usually curved), one uses a teasing needle and one uses a paint brush. 
It's up to the individual's technique but we do try to discourage using one's 
fingers too close to the blade.
Hope this helps.
 
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
 





From: Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Tue, March 8, 2011 8:20:16 AM
Subject: [Histonet] microtome safety

Morning all!

I need some quick responses to this question:  do you use your fingers or an 
instrument of some sort to pull your paraffin ribbons off the block when 
sectioning?  For those that do not use their fingers, what do you use?  If 
forceps, are these the typical lab forceps or a special type?

Thanks so much!

Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov



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CONFIDENTIALITY NOTICE
This message and any included attachments are from Somerset Medical Center
and are intended only for the addressee.  The information contained in this
message is confidential and may contain privileged, confidential,
proprietary and/or trade secret information entitled to protection and/or
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printing, copying, distribution, or use of such information is strictly
prohibited and may be unlawful.  If you are not the addressee, please
promptly delete this message and notify the sender of the delivery error
by e-mail or you may call Somerset Medical Center's computer Help Desk
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Be sure to visit Somerset Medical Center's Web site - 
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RE: [Histonet] microtome safety

[Sebree Linda A]

I've always used my fingers but some others in my lab use forceps. 


Linda A. Sebree
University of Wisconsin Hospital  Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Bartlett, Jeanine (CDC/OID/NCEZID)
Sent: Tuesday, March 08, 2011 7:20 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] microtome safety

Morning all!

I need some quick responses to this question:  do you use your fingers
or an instrument of some sort to pull your paraffin ribbons off the
block when sectioning?  For those that do not use their fingers, what do
you use?  If forceps, are these the typical lab forceps or a special
type?

Thanks so much!

Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov



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Re: [Histonet] microtome safety

[Rene J Buesa]

I always found better and used a wet camel's hair pencil. It provides the 
most gentle pull on the sections.
René J.
 
 

--- On Tue, 3/8/11, Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov wrote:


From: Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov
Subject: [Histonet] microtome safety
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date: Tuesday, March 8, 2011, 8:20 AM


Morning all!

I need some quick responses to this question:  do you use your fingers or an 
instrument of some sort to pull your paraffin ribbons off the block when 
sectioning?  For those that do not use their fingers, what do you use?  If 
forceps, are these the typical lab forceps or a special type?

Thanks so much!

Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov



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RE: [Histonet] microtome safety

[Patsy Ruegg]

Jeanine,

I use forceps with the tips bent at a 90 degree angle for reaching under the
sections to remove air bubbles.


Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, March 08, 2011 6:54 AM
To: histonet@lists.utsouthwestern.edu; Jeanine (CDC/OID/NCEZID)Bartlett
Subject: Re: [Histonet] microtome safety

I always found better and used a wet camel's hair pencil. It provides the
most gentle pull on the sections.
René J.
 
 

--- On Tue, 3/8/11, Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov wrote:


From: Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov
Subject: [Histonet] microtome safety
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date: Tuesday, March 8, 2011, 8:20 AM


Morning all!

I need some quick responses to this question:  do you use your fingers or an
instrument of some sort to pull your paraffin ribbons off the block when
sectioning?  For those that do not use their fingers, what do you use?  If
forceps, are these the typical lab forceps or a special type?

Thanks so much!

Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov



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RE: [Histonet] microtome safety

[Patsy Ruegg]

Actually I need to qualify my answer, I use my fingers to grab end of the
ribbon the farest from the blade and curved forceps near the blade so I use
both finger and forceps at the same time.

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-Original Message-
From: Patsy Ruegg [mailto:pru...@ihctech.net] 
Sent: Tuesday, March 08, 2011 7:16 AM
To: 'Rene J Buesa'; 'histonet@lists.utsouthwestern.edu'; 'Jeanine
(CDC/OID/NCEZID)Bartlett'
Subject: RE: [Histonet] microtome safety

Jeanine,

I use forceps with the tips bent at a 90 degree angle for reaching under the
sections to remove air bubbles.


Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, March 08, 2011 6:54 AM
To: histonet@lists.utsouthwestern.edu; Jeanine (CDC/OID/NCEZID)Bartlett
Subject: Re: [Histonet] microtome safety

I always found better and used a wet camel's hair pencil. It provides the
most gentle pull on the sections.
René J.
 
 

--- On Tue, 3/8/11, Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov wrote:


From: Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov
Subject: [Histonet] microtome safety
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date: Tuesday, March 8, 2011, 8:20 AM


Morning all!

I need some quick responses to this question:  do you use your fingers or an
instrument of some sort to pull your paraffin ribbons off the block when
sectioning?  For those that do not use their fingers, what do you use?  If
forceps, are these the typical lab forceps or a special type?

Thanks so much!

Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov



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RE: [Histonet] microtome safety

[kgrobert]

I use forceps, the thinner the tips, the better.  I've had people in here
who use brushes, too.  I can't use my fingers, the ribbons always stick.

Kathleen

Principal Lab Technician
Neurotoxicology Labs
Molecular Pathology Facility Core
Dept of Pharmacology  Toxicology
Rutgers, the State University of NJ
41 B Gordon Road
Piscataway, NJ 08854
(732) 445-6914

 Same here. One tech keeps her index fingernail extra long for this
 purpose.

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Sheila
 Haas
 Sent: Tuesday, March 08, 2011 8:26 AM
 To: Bartlett, Jeanine (CDC/OID/NCEZID);
 histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] microtome safety


 Every tech in my facility uses something different. Some use fingers,
 some use
 forceps (usually curved), one uses a teasing needle and one uses a paint
 brush.
 It's up to the individual's technique but we do try to discourage using
 one's
 fingers too close to the blade.
 Hope this helps.
  
 Sheila Haas
 Laboratory Supervisor
 MicroPath Laboratories, Inc.
  




 
 From: Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov
 To: histonet@lists.utsouthwestern.edu
 histonet@lists.utsouthwestern.edu
 Sent: Tue, March 8, 2011 8:20:16 AM
 Subject: [Histonet] microtome safety

 Morning all!

 I need some quick responses to this question:  do you use your fingers or
 an
 instrument of some sort to pull your paraffin ribbons off the block when
 sectioning?  For those that do not use their fingers, what do you use? 
 If
 forceps, are these the typical lab forceps or a special type?

 Thanks so much!

 Jeanine Bartlett, BS, HT(ASCP)QIHC
 Centers for Disease Control and Prevention
 Infectious Diseases Pathology Branch
 1600 Clifton Road, MS/G-32
 18/SB-114
 Atlanta, GA  30333
 (404) 639-3590
 jeanine.bartl...@cdc.hhs.gov



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 CONFIDENTIALITY NOTICE
 This message and any included attachments are from Somerset Medical Center
 and are intended only for the addressee.  The information contained in
 this
 message is confidential and may contain privileged, confidential,
 proprietary and/or trade secret information entitled to protection and/or
 exemption from disclosure under applicable law.  Unauthorized forwarding,
 printing, copying, distribution, or use of such information is strictly
 prohibited and may be unlawful.  If you are not the addressee, please
 promptly delete this message and notify the sender of the delivery error
 by e-mail or you may call Somerset Medical Center's computer Help Desk
 at 908-685-2200, ext. 4050.

 Be sure to visit Somerset Medical Center's Web site -
 www.somersetmedicalcenter.com - for the most up-to-date news,
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RE: [Histonet] microtome safety

[sgoebel]

I use the end (without bristles) of a paintbrush

Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Bartlett, Jeanine (CDC/OID/NCEZID)
Sent: Tuesday, March 08, 2011 7:20 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] microtome safety

Morning all!

I need some quick responses to this question:  do you use your fingers
or an instrument of some sort to pull your paraffin ribbons off the
block when sectioning?  For those that do not use their fingers, what do
you use?  If forceps, are these the typical lab forceps or a special
type?

Thanks so much!

Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov



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RE: [Histonet] microtome safety

[Sherwood, Margaret]

Just regular forceps. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett,
Jeanine (CDC/OID/NCEZID)
Sent: Tuesday, March 08, 2011 8:20 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] microtome safety

Morning all!

I need some quick responses to this question:  do you use your fingers or an
instrument of some sort to pull your paraffin ribbons off the block when
sectioning?  For those that do not use their fingers, what do you use?  If
forceps, are these the typical lab forceps or a special type?

Thanks so much!

Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov



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Re: [Histonet] microtome safety

[Jennifer MacDonald]

I use curved forceps to hold the ribbon and a paint brush to release it 
from the knife edge.  The curved forcepts help to separate the sections 
and remove air bubbles.

Jennifer MacDonald




Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov 
Sent by: histonet-boun...@lists.utsouthwestern.edu
03/08/2011 05:25 AM

To
histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
cc

Subject
[Histonet] microtome safety






Morning all!

I need some quick responses to this question:  do you use your fingers or 
an instrument of some sort to pull your paraffin ribbons off the block 
when sectioning?  For those that do not use their fingers, what do you 
use?  If forceps, are these the typical lab forceps or a special type?

Thanks so much!

Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov



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Re: [Histonet] microtome safety

[amitapandey]

We use fingers...I am not aware of any kind of forceps.
But along with you i am also curious to know if any thing else is 
available.


Amita


From:
Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov
To:
histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date:
08/03/11 06:52 PM
Subject:
[Histonet] microtome safety
Sent by:
histonet-boun...@lists.utsouthwestern.edu



Morning all!

I need some quick responses to this question:  do you use your fingers or 
an instrument of some sort to pull your paraffin ribbons off the block 
when sectioning?  For those that do not use their fingers, what do you 
use?  If forceps, are these the typical lab forceps or a special type?

Thanks so much!

Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov



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Re: [Histonet] Microtome for undecalcified tissues

[Pamela Marcum]

Depending on the size of the bone the Leica 2255 is a great mictorome to use 
with tungsten carbide blades.  It is heavy enough to larger blocks and is 
motorized.  If it is not motorized I would not try it for as that will give you 
the best results. 



Pam Marcum 




- Original Message - 
From: Adam Hacking ahack...@yahoo.com 
To: Histonet@lists.utsouthwestern.edu 
Sent: Friday, October 22, 2010 11:42:52 AM 
Subject: [Histonet] Microtome for undecalcified tissues 

Hi, 
  
I'm looking for a good used sledge or rotary microtome capable of cutting MMA 
emebdded bone specimens. Can anyone reccomend a good model, manufacturer, 
supplier or know of a good used machine ? 
  
Many thanks. 
  
Adam 



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- Original Message - 
From: Adam Hacking ahack...@yahoo.com 
To: Histonet@lists.utsouthwestern.edu 
Sent: Friday, October 22, 2010 11:42:52 AM 
Subject: [Histonet] Microtome for undecalcified tissues 

Hi, 
  
I'm looking for a good used sledge or rotary microtome capable of cutting MMA 
emebdded bone specimens. Can anyone reccomend a good model, manufacturer, 
supplier or know of a good used machine ? 
  
Many thanks. 
  
Adam 



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Re: [Histonet] Microtome alignment

[Jaime Plata]

Amos, indication it is the most practical answer. Histology it is a combination 
of skills, art, and science.
Jaime E Plata MD. MT. HTL (ASCP)  

--- On Wed, 8/18/10, Amos Brooks amosbro...@gmail.com wrote:

From: Amos Brooks amosbro...@gmail.com
Subject: [Histonet] Microtome alignment
To: sharon.davis-dev...@carle.com, histonet@lists.utsouthwestern.edu
Date: Wednesday, August 18, 2010, 6:57 PM

Hi,
     My favorite tool works on all microtomes and is priced perfectly. The
knobs on the block holder move the block so when it looks like you are going
to cut too much of a block you turn them and, voila, a perfectly aligned
microtome.

Amos


Message: 2
Date: Tue, 17 Aug 2010 14:16:26 -0500
From: Sharon.Davis-Devine sharon.davis-dev...@carle.com

Subject: [Histonet] Microtome alignment
To: histonet@lists.utsouthwestern.edu
       histonet@lists.utsouthwestern.edu
Message-ID:
       fe4513daaa85804d9a9fbac9c1ee7a668edbe...@exchangeccabe.carle.com
Content-Type: text/plain; charset=us-ascii

We are having a continuing issue of too much tissue being cut off when
facing off a block for recuts. We have tried a couple of different methods
for aligning our microtomes without much success.  Does anyone out there
have any advice on how to properly align them and what tool to use?  Also,
how often do you perform this re-alignment?  The majority of our microtomes
are older so more wear and tear and things move out of place more often. Any
help or suggestions will be greatly appreciated.  Thanks.

Sharon Davis-Devine, CT (ASCP)
Cytology-Histology  Supervisor
Carle Foundation Hospital
Laboratory and Pathology Services
611 West Park Street
Urbana, Illinois 61801
217-383-3572
sharon.davis-dev...@carle.com
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RE: [Histonet] Microtome alignment

[joelle weaver]

This is a learned histology skill, but there are some devices which can be used 
to align different microtomes available, and you can test your adjustments with 
blank blocks. Also newer microtomes have a device on the block holder that 
will align the position in the X-Y axis in the zero position. 
Joelle Weaver
 
 From: sharon.davis-dev...@carle.com
 To: histonet@lists.utsouthwestern.edu
 Date: Tue, 17 Aug 2010 14:16:26 -0500
 Subject: [Histonet] Microtome alignment
 
 We are having a continuing issue of too much tissue being cut off when facing 
 off a block for recuts. We have tried a couple of different methods for 
 aligning our microtomes without much success. Does anyone out there have any 
 advice on how to properly align them and what tool to use? Also, how often do 
 you perform this re-alignment? The majority of our microtomes are older so 
 more wear and tear and things move out of place more often. Any help or 
 suggestions will be greatly appreciated. Thanks.
 
 Sharon Davis-Devine, CT (ASCP)
 Cytology-Histology Supervisor
 Carle Foundation Hospital
 Laboratory and Pathology Services
 611 West Park Street
 Urbana, Illinois 61801
 217-383-3572
 sharon.davis-dev...@carle.com
 
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Re: [Histonet] Microtome alignment

[Paula Sicurello]

A while back I remember someone suggestion something like a right angle
device that carpenters use.  It's basically just a piece of metal that is a
right angle triangle that you put up against the chuck and on the knife
mount.  Then you align the chuck so it is a a right angle to the knife
mount.

It looks like this:l\
l  \
l\   This is my best attempt at computer
drawing.
l __ \

I don't thing they cost very much, much less that $700.


Paula  :-)



On Tue, Aug 17, 2010 at 8:05 PM, WILLIAM DESALVO
wdesalvo@hotmail.comwrote:


 Since you have older microtomes, I suggest using an alignment block at
 each microtome instead of purchasing the alignment tools. The tools can be
 found on the web  ttp://www.grale.com.au/products/view/804 , but they can
 be expensive (as much as $700.00 each). If you have more than one
 manufacturer for your microtomes, you will need to purchase one for each
 brand.

 Try using your largest embedding mold and make a blank block for each
 microtome. This can bee done first thing each morning. Use the block to
 align the chuck each morning before cutting. If you see drift throughout the
 day, add one or more checks during the day. Making a fresh block each day
 gives you a good standard and keeps the variation down.

 I also suggest you look at your embedding method and make sure you have a
 standardized procedure for all tissue types for orientation of tissue and
 exact placement in the mold. Embed your tissue on one plane with as little
 paraffin as possible on the bottom of the mold. Reducing variation at
 embedding will greatly assist you in reducing the amount of facing
 required to start producing sections and also reduce the need to align the
 chuck to the block/tissue.

 William DeSalvo, B.S., HTL(ASCP)
 Chair, NSH QCC
 Prodcution Manager, Sonora Quest Laboratories




  From: sharon.davis-dev...@carle.com
  To: histonet@lists.utsouthwestern.edu
  Date: Tue, 17 Aug 2010 14:16:26 -0500
  Subject: [Histonet] Microtome alignment
 
  We are having a continuing issue of too much tissue being cut off when
 facing off a block for recuts. We have tried a couple of different methods
 for aligning our microtomes without much success. Does anyone out there have
 any advice on how to properly align them and what tool to use? Also, how
 often do you perform this re-alignment? The majority of our microtomes are
 older so more wear and tear and things move out of place more often. Any
 help or suggestions will be greatly appreciated. Thanks.
 
  Sharon Davis-Devine, CT (ASCP)
  Cytology-Histology Supervisor
  Carle Foundation Hospital
  Laboratory and Pathology Services
  611 West Park Street
  Urbana, Illinois 61801
  217-383-3572
  sharon.davis-dev...@carle.com
 
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-- 
Paula Sicurello
6 of 6
Duke Healthcare System EM Lab
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