I had the same issue before. PAS should be a decent polymer for
modification.
On Thu, Nov 9, 2023 at 7:04 PM sadik sattar wrote:
> Hi Careina,
>
> I hope this email finds you well. I wanted to share my experience with a
> similar issue I faced in the past concerning one of my proteins, and I've
Dear Careina,
I usually see such kind of "Quasi-crystal" when the folding force was not
properly adjusted. Of course, you need to verify they are protein ppt
before any further modifications.
For these melon-seed-like crystals, the sharp-end indicated that some
crystallization occurred. However,
ing link:
> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1
>
> This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a
> mailing list hosted by www.jiscmail.ac.uk, terms & conditions are
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---
>
> -
> --
> Dr. Javier M. González
> Instituto de Bionanotecnología del NOA (INBIONATEC-CONICET)
> Universidad Nacional de Santiago del Ester
tallization?? How to find which concentration is
> enough for our target protein crystallization?? Thanks in advance
>
> --
>
> To unsubscribe from the CCP4BB list, click the following link:
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>
> To unsubscribe from the CCP4BB list, click the following link:
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--
Kevin Jin
Sharing knowledge each other is always very joyful..
Website: http://www.jinkai.org/
#
. I can send a script for that.
>
> Regards
> Garib
>
> P.S. In spite of some unusual answers you should not be afraid asking
> questions on ccp4 bb. This bulletin board is exactly for this type of
> questions.
>
>
> On 10 Sep 2018, at 18:01, Kevin Jin wrote:
>
&g
; --
>
> To unsubscribe from the CCP4BB list, click the following link:
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gt; I am particularly interested in those cryo-em structures with high
>>> resolution, like 2.6~2.8A.
>>>
>>
>> Sure, all are excited about high-res cryo-EM!!!
>>
>>
>>> Please give me an education.
>>>
>>
>> Sure. One of available universit
Dear All,
Is there any sever available to create electron density maps for cryo-em
structures? Or, we should create the maps from mmCIF. I am particularly
interested in those cryo-em structures with high resolution, like 2.6~2.8A.
Please give me an education.
Thanks,
Kevin
###
d how to purify the protein. The protein PI was 6.33
>
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>
> To unsubscribe from the CCP4BB list, click the following link:
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--
Kevin Jin
Sharing knowle
t., Mailstop 497
> Philadelphia, PA 19102-1192 USA
>
> (215) 762-7706
> pjl...@gmail.com
> pj...@drexel.edu
>
>
>
> To unsubscribe from the CCP4BB list, click the following link:
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il: krey.tho...@mh-hannover.de
>
>
>
> --
>
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--
Kevin Jin
Sharing knowledge each other is always very joyful..
il : mat...@itqb.unl.pt
> >
> >http://www.itqb.unl.pt/research/biological-chemistry/industry-and-medicine-applied-crystallography
> >http://www.itqb.unl.pt/labs/macromolecular-crystallography-unit
> >
> >Mailing address :
> >Instituto de Tecnologia Quimica e Biologica António Xavier
> >Universidade Nova de Lisboa
> >Av. da República
> >2780-157 Oeiras
> >PORTUGAL
> >
> >ITQB NOVA, a great choice for your PhD
> >https://youtu.be/de6j-aaTWNQ
> >
> >Master Programme in Biochemistry for Health
> >https://youtu.be/UKstDCFjYI8
>
>
>
--
Kevin Jin
Sharing knowledge each other is always very joyful..
Website: http://www.jinkai.org/
s to the resin.
> Thank you in advance
> Sent from my iPhone
--
Kevin Jin
Sharing knowledge each other is always very joyful..
Website: http://www.jinkai.org/
ling oxygen through and heating the sample to accelerate the
> oxidation process.
>
>
>
> King Regards
>
>
> Jonathan Bailey (PhD student)
>
>
> Professor Martin Caffrey Lab MS&FB group Trinity College Dublin
>
--
Kevin Jin
Sharing knowledge each other is always very joyful..
Website: http://www.jinkai.org/
ut
> concentration dependence.
>
> In each case, the protein precipitates to a milky solution within about a
> minute of removal from ice (I am working with 20-50 uL volumes in PCR
> tubes).
>
> Many thanks for any suggestions!
>
> Best,
> Chris
>
--
Kevin Jin
Sharing knowledge each other is always very joyful..
Website: http://www.jinkai.org/
him.
The advantage of their clone: first, high capacity and better column
performance(plate numbers).
On Fri, Feb 17, 2017 at 9:33 AM, Kevin Jin wrote:
> Probably you can try His-Column from Clonetech (Takeda ?). They modified
> the column packing using nylon membrane, which offered bette
Probably you can try His-Column from Clonetech (Takeda ?). They modified
the column packing using nylon membrane, which offered better flow-through
than that of traditional resin-gel column.
On Wed, Feb 15, 2017 at 8:57 AM, Markus Seeliger
wrote:
> Dear all,
> we are happy users of all that GE
t;
> I have tried to fit in diethylene glycol as shown in one of the attached
> figures, but as observed, it is not really fit and the molecule is in
> incorrect conformation.
>
> Kindly help me with this matter.
>
>
>
> Thanks and regards,
>
> Sharifah Nur Hidayah
Likely, the protein is pH sensitive.
You may chain the anion and cation columns together for protein separation,
then you don't need to use Imidazole.
P.S. Tris pH 7.5 (RT) is ~ pH 8.2 in cold room
On Sat, Dec 24, 2016 at 2:52 AM, Praveen Tripathi <
tripathipraveen2...@gmail.com> wrote:
> Dea
g-il probably means something like Gold Upright Sun. Just
> to demonstrate how poor those things translate into reality….
>
> ** **
>
> PS: Orcward ligand orcs, the Amt is watching!
>
> ** **
>
> PPS: it is still ok to ride a trolley.
>
> ** **
>
> *
The skill of presentation is at least as important in Science as being
> right.
>
> ** **
>
> Best, BR
>
> ** **
>
> *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of
> *Kevin
> Jin
> *Sent:* Tuesday, April 03, 2012 3:34 PM
&
Dear All,
Here may be another example for the importance of image storage.
http://www.jinkai.org/DERA/DERA_1O0Y_3R12.html
Regards,
Kevin
to answer.
*Je pense donc je suis*
Kevin
On Sun, Apr 1, 2012 at 8:09 AM, Paul Emsley
wrote:
> On 31/03/12 23:08, Kevin Jin wrote:
>
>
> I really wish PDB could have some people to review those important
> structures, like paper reviewer.
>
>
> So do the wwPDB, I wou
responsibility for the data of each
> other, as it happens in many fruitful collaborations between biologists and
> "crystallographers" (such as these I had the privilege to engage with
> collaborators that criticised my data, as I did theirs ...).
>
> regards to all -
>
> Tassos
>
> (and please, no 1st April joke with fraud cases !)
--
Kevin Jin
Sharing knowledge each other is always very joyful..
Website: http://www.jinkai.org/
lified.
> ..
> Jürgen Bosch
> Johns Hopkins University
> Bloomberg School of Public Health
> Department of Biochemistry & Molecular Biology
> Johns Hopkins Malaria Research Institute
> 615 North Wolfe Street, W8708
> Baltimore, MD 21205
> Office:
enough.
5. Most of Phosphate buffer should be removed.
That's what I used before.
Let me know if it works.
Best,
Kevin Jin
On Fri, Mar 30, 2012 at 9:40 AM, Afshan Begum wrote:
>
> Dear Kevin
>
> I have seen your website and come to you to discuss my problem actual
Dear All,
Here is way I have used for protein and hydrophobic organic compound
cocrystallization. Via this method, less amount of DMSO will be used
to aviod protein ppt.
I hope you can use for your research.
http://www.jinkai.org/Xtal.html
Regards,
--
Kevin Jin
Sharing knowledge each other
Dear All,
Making the surface of cover glass hydrophilic could let drop with
organic solvent (MPD, etc) shrink into a ball shape, not spread out.
Here is the method I used before.
http://www.jinkai.org/Xtal.html
Kevin
--
Kevin Jin
Sharing knowledge each other is always very joyful
Dear All,
I developed a method to remove endotoxin before and it worked pretty
well. Here is the strategy for this protocol.
http://www.jinkai.org/Endotoxin.html
I hope it is useful.
Best,
Kevin
--
Kevin Jin
Sharing knowledge each other is always very joyful..
Website: http
stions or any kind of details or a paper that might help I
> will be thankful
>
> Best Regards
> Rana
--
Kevin Jin
Candle always burns itself out to light up the tunnel .
Website: http://www.jinkai.org/
Dear All,
Maybe most of you already knew. I just put my understanding of SDS
page gel storage here, if you want to make gel yourself and safe some
money.
http://www.jinkai.org/SDS_page.html
If I am wrong, please let me know.
Sharing knowledge each other is always joyful.
--
Kevin Jin
Protein
Dear All,
Do you need some help for structure refinement or structure analysis?
I will be very happy to work for you, while I am looking for next
position.
To me, solving structure is a puzzle game and for fun.
Regards,
Kevin Jin
AM, Kevin Jin wrote:
> Hi Min,
>
> Please try this way if you use your protein for crystallization.
>
> 1. collect the needle and run SDS page or FPLC to verify the presence
> of protein. Make sure it is not a buffer salt.
>
> 2. You don't need to do dialysis to r
Best,
Kevin
On Tue, Mar 13, 2012 at 8:36 AM, Min-Kyu Cho wrote:
> Thank you Kevin,
>
> I will try to remove b-ME as you suggested.
>
> Min-Kyu
>
> | -Original Message-
> | From: Kevin Jin [mailto:kevin...@gmail.com]
> | Sent: Monday, March 12, 2012 5:50
r 12, 2012 1:11 PM, "Min-Kyu Cho" wrote:
>>
>> I am using KPi buffer at pH 5.5, 100mM KCl, 2mM beta-mercaptoethanol,
>> 0.02%
>> NaN3.
>>
>> Yes, I agree I should check CD melting curve to see temperature preference
>> of my protein.
&
Which kind of buffer you use? If it is Tris, then temperature change
will cause pH change.
Actually, this is a good way for crystallization.
Kevin
On Mon, Mar 12, 2012 at 9:02 AM, Min-Kyu Cho wrote:
> Hi all,
>
> I have a homotetrameric coiled-coil domain sample with 45aa per each. While
> I st
ne achieve this by buffer exchange?
>
> I wonder if an extensive wash of protein on a column would work
> instead.
>
> Pius
>
> On Thu, Mar 8, 2012 at 4:01 PM, Kevin Jin wrote:
>> To Pius,
>>
>> In my case, the protein/biopolymer is Lysine/amine riched.
>&
can test my strategy and improve it. If you can give me a
feed back, there will be great!
Kevin
On Thu, Mar 8, 2012 at 1:01 PM, Kevin Jin wrote:
> To Pius,
>
> In my case, the protein/biopolymer is Lysine/amine riched.
>
> 1. Lys or Arg are mainly positively charged under pH 7.0 ~
To Pius,
In my case, the protein/biopolymer is Lysine/amine riched.
1. Lys or Arg are mainly positively charged under pH 7.0 ~ 8.0, and
then provide positively charge to LPA. Of course, some metal cations
could also be involved in LPA binding.
2. Tris with the NH2 group could also be protonated
I tried to use 250mM Tris pH 7.6, 1M NaCl and 5~10% 2-propanol for
dialysis. It works,
On Wed, Mar 7, 2012 at 6:50 AM, Jerry McCully
wrote:
>
>
> Dear All;
>
> We purified a His tag protein by Ni-NTA and gel-filtration from
> E.coli.
>
> We tried two endotoxin removal resins fr
Maybe you can this way.
Use Na acetate to elute your protein, then use EDTA to remove the Ni
from your protein, then buffer exchange or dialysis to remove EDTA.
Kevin
On Fri, Mar 2, 2012 at 5:50 AM, Santosh wrote:
> Hi Anita,
> As Artem noted, use of Histrap column at lower pH would not be a
Dear All,
I wrote a story for the catalysis of Orotidine 5'-Monophosphate
Decarboxylase.
http://www.jinkai.org/Enzymology.html
I hope you will like it.
Regards,
Kevin
I collected GTP/Mg2+ crystal on SSRL beamline 9-1 before. The images
was processed by Mosflm and structure was solved by Shelx as usual.
Kevin
On Wed, Feb 8, 2012 at 3:41 AM, Giorgio Giardina
wrote:
> Hello,
> I have some interesting small molecule xtals.
> I was wondering if it is possible to
3
> FAX: 706-542-1738
> ***
>
>
>
>
>
>
> On Feb 7, 2012, at 11:22 AM, Kevin Jin wrote:
>
>> As we know, the pKa of water is 15.7. Under pH 7.0, its protonation
>> should be 50/50.
>> In this case, w
Maybe you would also be interested in
http://www.jinkai.org/AAD_history.html
Regards,
Kevin
On Tue, Feb 7, 2012 at 8:52 AM, Christian Roth
wrote:
> Hi,
>
> you may also look into the papers of John A. Gerlt, who did a lot on
> protonabtraction reactions and the theory behind this. Esspecially
pKa perturbation. I am also
interested in the general base of Asp and Glu in enzyme catalysis.
I will be very happy to read your paper in the future.
Regards,
Kevin Jin
On Tue, Feb 7, 2012 at 3:48 AM, Deepak Oswal wrote:
> Dear colleagues,
>
> We have solved the crystal structure o
Maybe, you can adjust the ion strength of your condition.
On Thu, Jan 26, 2012 at 8:32 AM, Kevin Jin wrote:
> For crystallization:
>
> Your xtal may come out a little bit fast. If the condition contain
> alcohol, such as IPA, you may have to modify it.
>
> If you l
For crystallization:
Your xtal may come out a little bit fast. If the condition contain
alcohol, such as IPA, you may have to modify it.
If you let people know the condition, it may be more helpful.
Also, please check the purity of your protein.
Kevin
On Thu, Jan 26, 2012 at 7:33 AM, Theresa
Dear All,
I got this email. Is my account blocked?
Thanks,
Kevin
2012/1/24 :
> Esta cuenta de correo electrónico dejara de existir dentro de 6 meses
> (25/05/2012).
>
> Si desea ponerse en contacto con el titular de este correo hágalo a través
> del siguiente e-mail: annie_sch...@yahoo.de
>
Dear All,
I wrote a story about the catalysis of Acetoacetate Decarboxylase. It
is available here:
http://www.jinkai.org/AAD_history.html
http://www.jinkai.org/AAD_catalysis.html
I wish you will like it.
Regards,
Kevin
I did not make it clear. The protocol we used for protein expression always
limit the occ of MSE as 0.75. You may estimate your occ of MSE by ED. You
can check PDB for JCSG structures.
Cheers,
Kevin
On Fri, Sep 16, 2011 at 7:09 AM, Ming wrote:
> Hi all,
>
>
> I was using Refmac from CCP4 to re
e file is not a PIR file. The file looks OK to me, but I've never
> really understood what a PIR file is
>
> Phil
>
> On 12 Aug 2011, at 01:39, Kevin Jin wrote:
>
> >
> > Should we really have some crystallographers to review and qc those
> structures before the
>
>
>
> Can anyone get this server to work? For me it keeps complaining that my
> sequence file is not a PIR file. The file looks OK to me, but I've never
> really understood what a PIR file is
>
> Phil
>
> On 12 Aug 2011, at 01:39, Kevin
Should we really have some crystallographers to review and qc those
structures before the formal releasing? JCSG has set a very good mechanism
for this issue.
There is a sever for self check.
http://smb.slac.stanford.edu/jcsg/QC/
On Thu, Aug 11, 2011 at 4:58 PM, Jacob Keller <
j-kell...@fsm
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