[Histonet] Paraffin Block triming Thanks for the info

Well thanks for all of the input. We have always scraped the blocks with an empty cold basemold, and it works fine. I had recieved ane-mail advertisment from one of our suppliers, it had a picture of the TBS brand Shur Trim block trimmer. I was just thinking it might save a little time,

[Histonet] van Giesons for Sanderson's Rapid Bone Stain can be Unna's modified VG

Unna's modified van Gieson's stain for the Rapid Bone Stain is reported to give deeper colors than the VG recipe/solution commonly found in histotechnology textbooks. It contains a higher concentration of dyes but also contains nitric acid which is going to acid etch the surface of the bone a bit.

RE: [Histonet] Paraffin Block trimming

No, We find them faster and we believe safer Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, West

RE: [Histonet] Paraffin Block triming

We also have a paratrimmer and agree with Pam Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, West

RE: [Histonet] Sanderson's Rapid Bone Stain

One other counterstain can be used also. Alizarin Red S for the calcium as long as acid etching is not performed on the surface of thick sections. I would presume von Kossa could be used also if Alizarin Red S has been done in past. Gayle Callis -Original Message- From: histonet-boun

[Histonet] list of special stains

Does anyone have or know where to obtain a list of special stains that briefly describes which each one is for? Thanks, Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 _

[Histonet] free Tissue Tek II vacuum infiltrator

I have an old Tissue Tek II vacuum infiltrator free to a good home. Only cost will be shipping via ground..either FedEx or UPS. Cathy Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://li

AW: [Histonet] Re: processing v-e-r-y tiny samples

Our cytolab produces cytoblocks with plasma, that they bring to coagulation. The coagel is a smooth droplet in the size of 5-10 mm diameter. I could imagine, that the GI can be caught in such a coagel, and then be placed in a mesh-cassette. Gudrun Lang ___

[Histonet] Free- Floating Frozen Brain Sections

Hi: I am new to this Forum and also to Immunohistochemistry of frozen sections. I want to use Floating Frozen brain sections to do some of immunohisto and immunofluorescence study. I will be grateful to you if you could provide me detail protocol for free floating brain sections. I meant fixation,

[Histonet] Re: processing v-e-r-y tiny samples

Andi, We process E7.0 mouse embryos and have problems sometimes because they are so very tiny and fragile. We've wrapped them and sometimes (but not all the time) had them break and flatten. Usually we use the histoscreen cassettes. They still will sometimes break apart using these but we have

RE: [Histonet] processing v-e-r-y tiny samples

Andi only gets the fun stuff!!! Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-bou

[Histonet] RE:Sanderson's RBS image

Thanks Gayle, I also looked at the Surgipath image, my slide was much more brilliant then that one. I did use the acid fuchsin counter stain. I just wasn't sure if mine would be considered 'over stained.' I'll send you an image that Jack Ratliff sent me that compares to what I was seeing to

Re: [Histonet] processing v-e-r-y tiny samples

I may never complain about tiny GI or bronch biopsies ever again! >>> Andrea Grantham 3/19/2009 11:51 AM >>> Good Morning! In keeping with the weirdness of the projects I get in this lab today my question is about processing mosquito GI tracts. I have a processing schedule - that is not the prob

[Histonet] Double-stain for immune cell markers

Hi Naira, Try Biocare. They make fantastic reagents for research as well as some great double-stain kits. As for the antibodies, it looks like you might have to create these either as cocktails (a lot of front-end work) or just make your stain longer (a lot of overall work) by staining sepra

[Histonet] Sanderson's Rapid Bone Stain

Dear Fellow Histonetters, There are 3 counterstains that can be used with the Sanderson's Rapid Bone Stain (RBS). We primarily used the acidified acid fuchsin, however, we had clients that only used a van Gieson counterstain. Light green can also be used. We normally stained for 1 minute g

Re: [Histonet] Mosquito GI Tract

Actually I had a project to find bacteria in the ovaries of drosophila. The bacteria looked a lot like mitochondria and about the same size. That was a lot of fun, but at least I got to use a TEM for the imaging. You could barely see the sample in the dissecting microscope and it was only after I h

Re: [Histonet] dehydration

It seems that you are missing the "clearing" step or antemedium in the sequence you describe but, the tissue should not stay too long in neither alcohol (unless it is 2-propanol) or in the antemedium (unless it is mineral oil) in which cases the time in them is not an issue. René J. --- On Thu,

[Histonet] Re:Mosquito GI Tract

Hi Sara,   I would try to wrap the tissue placing it in the center of a piece of smooth lens paper carefully folding it so the tissue stays in place and can be easily and carfully unwrapped. Try to make a small packet not much too much larger than the tissue. If you can use a drop of eosin on t

[Histonet] Fluoro Jade Query

What's the latest and greatest on Fluoro Jade for degenerating neurons? I've found a few references, but they are old - - so am I - just celebrated my birthday - - - - anyway - -who's got the best answer? Winner gets a Hershey Bar! Jackie ___ Histone

Re: [Histonet] Mosquito GI Tract

More interesting - why is someone even looking at the mosquito GI tract? What happens in there that we need to know about? I guess for all the diseases those filthy beasts carry - they must harbor viruses and bacteria somewhere. Did you know that mosquitos did not exist in Hawaii until a lit

Re: [Histonet] Mosquito GI Tract

lol...I wanted to say the same thing...and how in the world can you even see it when you section it? How do you stain it without losing it from the slide? Is there really enough surface area for it to adhere and withstand washing? ...and it's ALMOST Friday...! --On Thursday, March 19, 2009 12:

[Histonet] dehydration

In an emergency situation (like not enough hrs in a day) - how long can tissue remain in the final ETOH before going into the paraffin embedding stage? I do all my work manually - no automation here!! Individuals who have received this information in error or are not authorized to receive it

[Histonet] RE: Histonet Digest, Vol 64, Issue 33

Hi Patty and all Histoneters, I am an independent histology equipment repair technician, based in the Atlanta, GA. Sometimes I acquire used equipment for resale and right now I have: 1) Leica Autostainer XL 1) Leica CV 5000 cover slipper Others can give testament to the quality and reliability

RE: [Histonet] Paraffin Block triming

I use the minimum amount in the base mold then top it off after I add the cassette top. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.u

[Histonet] RE:cassette labeler

Our techs and pathologists love our Leica cassette and slide printers. We've had them for over 2 years now, with few problems. The cassette labeler holds up to 6 different colors, and has an additional manual feed for anything special. The slide holds up to 3 different slide types, and also has

RE: [Histonet] Paraffin Block trimming

I've noticed that if I put a little extra pressure on the cassette on top of the mold when putting in the liquid paraffin it doesn't seem to seep out the bottom sides and therefore cleaner blocks. The fill level in the cassette matters too. I usually fill mine about 3/4 to the top of the cassett

AW: [Histonet] Paraffin Block triming

I think, it depends on the amount of paraffin you fill in the mold, before you put the cassette on it. If it is a little too much, you press the paraffin out. Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouth

[Histonet] RE: Paraffin Block Trimming

We use a paraffin block dewaxer in our lab. It works great. I find it saves time and doesn't create as much of a mess (with wax shavings). Some people in our lab still prefer to trim the excess wax with a blade or forceps. I guess it's all personal preference.

[Histonet] Mosquito GI Tract

And here I thought I had an unusual project today, finding out as I did that my boss is thinking of examining up to 1000 cattle for TB (at 10 lymph nodes/beast) using AFB and asking me to cost it for him. I think the Mosquito GI Tract Processing and Embedding question has my project beat! How wou

RE: [Histonet] Paraffin Block triming

I think it depends on whether you place your cassette bottom side down (slotted side close to the tissue) or bottom side up (open side close to the tissue) when embedding. The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is a

[Histonet] Marilyn A Weiss is out of the office as of 10 a.m. 3/19/2008

I will be out of the office starting 03/19/2009 and will not return until 03/24/2009. I will respond to your message when I return. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Paraffin Block triming

I think we all develop our own methods based on what we were taught and what becomes easiest or best for us. I do agree with Gayle as the fingers get older the use of these new devices is better and easier on us. We often build blocks here as a research lab with large pieces of tissue or decal

Re: [Histonet] PSLIM slide printer

Andi, How are you utilizing the printer? We are trying to incorporate the printer with real time printing as the tech scans the bar coded cassette. We have had one tech working with the PSLIM and they have printed about 200 slides over a couple of days, but it has been getting progressively w

RE: [Histonet] Paraffin Block triming

I also use mine warm Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Thursday, Mar

RE: [Histonet] Paraffin Block triming

I disagree. I take my molds right out of the holding chamber and use warm and have hardly any leakage and don't have to hardly scrape. However, the other techs do have leakage and have to scrape alot off. I think it is just in the embedders technique. Dawn -Original Message- From: histon

RE: [Histonet] Paraffin Block triming

Exactly right... And you can melt your blocks on the embedding center. The Thermo Paratrimmer is great tho. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: hi

[Histonet] RE: Paraffin block trimming device - the cheap method

We coveted the ParaTrimmer for years, but could not afford to buy one. We simply use a smaller size laboratory metal hot plate, propped it into a metal pan so the hot plate is at an angle suitable to allow the melted paraffin to run onto paper towels in the pan. This is one hot plate we donate t

RE: [Histonet] RE: Sandersons rapid bone stain photo

Maybe the NSH hard tissue committee has a picture. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:

[Histonet] RE: Sandersons rapid bone stain photo

On looking at the Surgipath photo, the bone is very pale although the osteocytes, osteoid and soft tissues look good. I am not sure what counterstain (two are touted in the stain data sheet) was used as seen in this photo. One is a basic fuchsin that turns the bone red. Be careful of overstaini

RE: [Histonet] Paraffin Block triming

I think the amount of excess paraffin depends on the temperature of the mold when you are embedding. I like my molds "cold" at room temperature and don't have much leakage whereas techs who keep the molds "hot" have a higher amount of leakage around the edges. Doug Showers, MS, HT Histology Manag

[Histonet] Seeking reference lab for Langerin glycoprotein IHC staining

One of our pathologists is looking for a reference lab to perform Langerin Glycoprotein IHC staining on FFPE human tissue. Any and all responses are welcome. Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608

[Histonet] PSLIM slide printer

I think mine was probably the last lab to purchase a PSLIM from AccuPlace before they sold off the printer to Fisher. Just in time! For what it is worth, I do like the printer. It is great for a small lab and is saving what is left of my wrist. However, we are on the second machine. The first o

Re: [Histonet] processing v-e-r-y tiny samples

Jeff - This is not an early April Fools joke. I don't want to wrap them so that they don't get really flattened out. I'm thinking to put them in histogel or agar now to make the orientation easier when embedding. Good idea? Andi At 08:51 AM 3/19/2009, Andrea Grantham wrote: Good Morning!

[Histonet] Xmatrx (Rx)

Histonet, Has anyone heard of this machine? We recently had a BioGenex sales person come and introduce it to us. I never knew that it existed, I’ve primarily used biogenex for antibodies. The literature looks interesting, it potentially can do IHC, CISH, SS, FISH, TUNEL on FFPE, Cellular prep

RE: [Histonet] Paraffin Block trimming

I guess one has to go to histology school to learn how to do it, then (sent under separate cover from another histonetter...) I 'm too researchy. ;-) --On Thursday, March 19, 2009 11:45 AM -0400 "Bartlett, Jeanine (CDC/CCID/NCZVED)" wrote: We have used a variety of cassettes and paraffin

RE: [Histonet] Paraffin Block triming

Jeanine, We had the best Chief instructor Billie Swisher. I am so very thankful that I got the training from that school. I do miss you guys Shakun -Original Message- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:j...@cdc.gov] Sent: Thursday, March 19, 2009 8:45 AM To: Shakun Aswan

Re: [Histonet] processing v-e-r-y tiny samples

i use those tiny mesh inserts for small biopsies, or in a pinch, i just wrap the tissue in a square of lint-free lens-cleaning paper... Andrea Grantham wrote: Good Morning! In keeping with the weirdness of the projects I get in this lab today my question is about processing mosquito GI tracts.

[Histonet] processing v-e-r-y tiny samples

Good Morning! In keeping with the weirdness of the projects I get in this lab today my question is about processing mosquito GI tracts. I have a processing schedule - that is not the problem. I'm wondering if anybody out in histoland has a suggestion for what kind of cassette to use. I was thin

RE: [Histonet] Paraffin Block trimming

We have used a variety of cassettes and paraffin over the years...same result. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: Merced Leiker [mailto:lei...@buffalo.edu] Sent: Thursday, March 19, 2009 11:16 AM

RE: [Histonet] Paraffin Block triming

Of course Shakunyou trained me! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: Shakun Aswani [mailto:shakun.asw...@acologix.com] Sent: Thursday, March 19, 2009 11:09 AM To: Bartlett, Jeanine (CDC/CCID/NCZ

[Histonet] dako autostainers

We are looking into buying the Dako Autostainer Plus. I asked our rep if they still have any older models around to look into. He made it sound like there are older models but, the stainer itself has stayed the same, just the software has changed/improved. He said they don't even like selling the

[Histonet] tissue processors/UK

Best low volume tissue processor, microwave or conventional, mainly lung biopsies in the short term, any ideas chaps??, thanks. Cheers

[Histonet] RE: Conversion of histo lab to Lean Sigma

Hi histo-netters, Our lab is trying to go Lean and I was wondering if anyone out there has done the conversion yet? Do you have any pointers or suggestions on how to make the transition easier. Our Department is in operation from 7am to 6:30pm. Thanks and have a great day This information is dir

Re: [Histonet] Paraffin Block trimming

I don't normally embed or cut my own sections anymore, but when I was doing it a few years ago on a larger scale (but still in a research setting), clean embedding generally did the trick. But when I did need to remove the excess wax on the sides of the cassette, I would melt it using a hotpla

RE: [Histonet] Paraffin Block trimming

hmmm...I do too...Fisher 15-182-505B HistoPrep Stainless-Steel Base Molds...maybe it's the cassette design or type of wax used? (VWR 15147-839 POLYFIN, VWR 87002-362 HISTOSETTE Cassettes, Simport Plastics Biopsy Cassettes) --On Thursday, March 19, 2009 10:57 AM -0400 "Bartlett, Jeanine (CDC/

RE: [Histonet] Paraffin block - cassette cleaner

We use the ThermoFisher model also. It keeps the floor much cleaner, too, since the paraffin is melted into a small cup rather than flying around the counter and floor. Doug Showers, MS, HT Histology Manager ProPath 8267 Elmbrook Dr. Suite 100 Dallas, TX 75247 To learn more about ProPath, plea

[Histonet] Paraffin block - cassette cleaner

Hello, We have the Thermo Fisher model as well and love it. It does an excellent job on the blocks.in much less time than traditional hand scraping. Karen J Kay, MLT Pathology Supervisor,Chinook Health Laboratory Chinook Regional Hospital,Lethbridge, Alberta, CANADA -Original Message-

Re: [Histonet] Paraffin Block trimming

The lab I work at part time uses the new paraffin trimmer by Surgipath, it is alot bigger than the one I have seen before in other labs. You can do approx. 8 blocks at a time. But I also agree, if you "clean" embedd, you do not have all that extra paraffin to clean up. Kathy Hicks H.T. (AS

RE: [Histonet] Paraffin Block triming

I am also one of that -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin

Re: [Histonet] Sanderson's RBS Image

> Does anyone have a picture of what a slide stained w/Sanderson's Rapid Bone should look like? No, but Google does. About a half a second of searching found these... http://www.surgipath.com/products/itm_photos/p111d26-large.jpg http://archotol.ama-assn.org/cgi/reprint/129/10/1125.pdf Suzan

RE: [Histonet] Paraffin Block trimming

Don't know what to say.I fill mine up and as long as I don't get to rough with it (bump it or something where it sloshes) I don't have any excess paraffin around the edges. I use metal base molds. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@c

[Histonet] Sanderson's RBS Image

Hi, Does anyone have a picture of what a slide stained w/Sanderson's Rapid Bone should look like? Thanks in advance, Suzanne ___ Suzanne Bruce, R.V.T. Histologist & Necropsy Coordinator

RE: [Histonet] Paraffin Block trimming

Now I'm interested in how you embed so as to not have to scrape...if i don't add enough wax (enough to end up rising around the edges of the cassette, hence the scraping later), i don't get a secure hold of the block to the cassette... ML --On Thursday, March 19, 2009 7:37 AM -0700 "Martin, G

FW: [Histonet] RE: Automating muscle and neuro stains

Have I died and gone to histologists heaven? Automate an ATPase, now there would be something special. No more crying and wailing over failed or partially successful acid reversal. I'll have to retreat to a darkened room, I'm traumatised. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S.

RE: [Histonet] Paraffin Block trimming

No you're not the only one ... I was wondering the same thing ... why all the scraping. It seems to me that clean embedding does the trick with a few exceptions. G -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On

RE: [Histonet] Paraffin Block trimming

We have one timmer. I don't use it, I hand trim simply because I am quicker with it. The other techs use the timmer to save their hands. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 From: h

Re: [Histonet] Paraffin Block trimming

I agree that this is quicker than manually---you can do 4?or 5 blocks at a time. -Original Message- From: Karin Groeger To: Pamela Marcum ; Angela Bitting ; histonet-boun...@lists.utsouthwestern.edu; Jennifer MacDonald ; Scott Cc: Histonet@lists.utsouthwestern.edu Sent: Thu, 19 Mar 20

Re: [Histonet] Paraffin Block trimming

My techs use our Para Trimmer from Thermo Scientific Shandon. It will save your hands, but it takes a lot more time than the old fashion hand scraping. I prefer to hand scrape. I can scrape at least twice as many by hand than with the para trimmer. Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Har

RE: [Histonet] Paraffin Block triming

I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.ut

[Histonet] Paraffin Block triming

I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP   From: Peter Carroll T

RE: [Histonet] Paraffin Block trimming

In my experience the paraffin block trimmer is quicker and easier on your hands than the old manual scraping method. Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 From: histonet-boun...@lists.utso

RE: [Histonet] Paraffin Block trimming

They work great for us and saves the hand. We use the Shandon Para Trimmer. Our techs love them. Karin Groeger Histology Supervisor US LABS, Irvine,CA 949-450-0145 ext. 649 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthweste

Re: [Histonet] Paraffin Block triming

> Does anybody use a paraffin block dewaxer ? Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work?

RE: [Histonet] Paraffin Block trimming

Actually the warm up time is about 3 minutes and after that I can do blocks much faster and cleaner than scraping. I can't imagine it being slower to be honest. We love it as it save a lot of time. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic

Re: [Histonet] Paraffin Block trimming

You will have to time worself as I did to find out that waiting fo the paraffin to melt in order to eliminate it takes 2.5 times more time than doing it manually with a pocket knife (as I used). Manually = 32 secs/block vs. 80 secks./block René J. --- On Thu, 3/19/09, Angela Bitting wrote: Fr

Re: [Histonet] Paraffin Block trimming

I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this emai

RE: [Histonet] RE: Automating muscle and neuro stains

I was hoping to determine the level of interest in having muscle and neuro stains automated? I am speaking with a vendor about the desire to automate many of my stains: ATP, NADH, SDH, COX, Acetyl cholinesterase, Luxol Fast Blue, etc. Would your lab use this technology? Would you do

Re: [Histonet] Paraffin Block triming

Hi Scott, We have one from Thermo Fisher (Shandon)and love it. It saves time and fingers in cleaning cassettes. I also use it to trim and shape large blocks for sectioning. No shavings no razor blades. Pam Marcum - Original Message - From: "Scott" To: Histonet@lists.utsouthwestern