Re: [Histonet] Aqueous mounting media

2011-08-16 Thread Markus F. Meyenhofer
The un-coupled Naphthol salts (from the phosphatase reaction, dissolved in the lipids of the tissue) degrade and cause N2 bubbles . We solved this problem (in the 60ties!)with a post treatment after the reaction: After the reaction, rinse in dist water, post fix in formalin 1:4 parts water (just

Re: [Histonet] Aqueous mounting media

2011-08-16 Thread Emily Sours
Sorry let me send that again with a link *http://tinyurl.com/3uw5lcm *I have no idea why we use this particular medium. * * A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Tue, Aug 16, 2011 at 9:40 P

Re: [Histonet] Aqueous mounting media

2011-08-16 Thread Emily Sours
We use Gel Mount A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Tue, Aug 16, 2011 at 2:48 PM, Kevin Bennett wrote: > All, > Can anyone recommend a quality aqueous mounting media? I was using Aqua

Re: [Histonet] RE: H&E on Frozen

2011-08-16 Thread Emily Sours
I concur on this. If my boss will let me, I can send you our elaborate protocol for H&E staining, It involves many dishes and many washes. But your H&E will be perfect. I don't have the protocol with me right now,as I'm at home but tomorrow I will ask about sending you the protocol. I don't see

[Histonet] Art Challenge

2011-08-16 Thread Matt Mincer
Hey All, I have been working on updating the look of our website and booth for NSH. The graphic design people I am working with don't seem to be getting the look I am going for. This gave me an idea. I would like to challenge all of the crafty Histoneters to draw, paint or Photoshop (or whate

Re: [Histonet] Aqueous mounting media

2011-08-16 Thread Loralei Dewe
Probe mount aqueous based mounting media from Innovex. My personal favorite!! Loralei Dewe On Tue, Aug 16, 2011 at 12:05 PM, Montina Van Meter < montina.vanme...@pbrc.edu> wrote: > ProLong Gold (Invitrogen) > > > > > > > Montina J. Van Meter, HT (ASCP) > Lab Manager > Dept. of Autonomic Neuros

RE: [Histonet] Aqueous mounting media

2011-08-16 Thread Montina Van Meter
ProLong Gold (Invitrogen) Montina J. Van Meter, HT (ASCP) Lab Manager Dept. of Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsou

RE: [Histonet] Aqueous mounting media

2011-08-16 Thread McMahon, Loralee A
We have been using an aqueous mounting media from Diagnostic Biosystems. http://dbiosys.com/ Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.

[Histonet] RE: H&E on Frozen

2011-08-16 Thread Rathborne, Toni
Our protocol is to: Use a positively charged slide Fix in 10% formalin for 1 minute Rinse in tap (a few dips) Gill 3 for 1 minute Rinse in tap (a few dips) 3-5 dips in bluing Rinse in tap (a few dips) Eosin 2-3 dips 100% ETOH (a few dips) 100% ETOH (a few dips) Xylene (dip until clear) Mount with

[Histonet] Aqueous mounting media

2011-08-16 Thread Kevin Bennett
All, Can anyone recommend a quality aqueous mounting media? I was using Aquatex from EMD chemicals but it's been discontinued, tried Aqua-Mount from Thermo but show air bubbles a few days after coverslipping. Thanks, Kevin Bennett HT ___ Histonet mailing

RE: [Histonet] Details about Masson Trichrome Stain !

2011-08-16 Thread Weems, Joyce
http://stainsfile.info/StainsFile/dyes/26905.htm This says 66... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [

[Histonet] Scorched tissue

2011-08-16 Thread Tammy Pickles
Hi, I am wondering if anyone can help me out with some processing issues that our lab has been having. We recently have been having reagent contamination issues with alcohol and xylene, where our tissues are soft and cloudy after processing. It seems that the reagent valves on the processor we

[Histonet] Details about Masson Trichrome Stain !

2011-08-16 Thread 连 王
Details about Masson Trichrome Stain !   Biebrich Scarlet: Biebrich scarlet 2.7 gm Acid fuchsin 0.3 gm Distilled water 300.0 ml Glacial acetic acid 3.0 ml   I am not sure what the Biebrich Scarlet here is ? Acid Red 66 or acid Red 73 ?   Acid Red 73 http://www.sigmaaldrich.com/catalog/ProductDetai

[Histonet] use of isopentane substitutes for tissue freezing

2011-08-16 Thread Lemke, Nancy
Hello all, I am considering purchasing a freezing unit through Thermo that can be used with either isopentane of a new solution, HFE-7000 that is sold with the unit. I need to be able to have frozen samples that allow high quality DNA and RNA extracted. Does anyone have any experience with th

[Histonet] Toxoplasma Control

2011-08-16 Thread Coppin, Margaret
Hello everyone, Thanks for the response regarding the Toxoplasma control. It's nice to have such a supportive network available for these types of needs. Margaret Margaret G. Coppin, HT(ASCP) Technical Supervisor--Immunohistochemistry ARUP Laboratories 500 Chipeta Way Salt Lake

[Histonet] Peloris Rapid tissue processor

2011-08-16 Thread Rene J Buesa
Dear Colleagues: I have just received the following e-mail from Leica Microsystems. It is evident that I was wrong and that now the tissues in the Peloris instrument go directly from the 2-propanol to the parafin wax and are not air dried. I just wanted to share this correction with you. René J. 

[Histonet] H&E on Frozen

2011-08-16 Thread Reynolds,Donna M
We do this frequently. Fix slides in Methanol (45ml) + formaldehyde (5ml) for 10 minutes. Wash several changes of tap water Stain with Harris Hematoxylin for 3 minutes Proceed as you would for paraffin sections Get great staining. I am sure if you are using a different Hematoxylin you could use

Re: [Histonet] protocol for in situ RT_PCR

2011-08-16 Thread Adam .
I've used 10% zinc buffered formalin from Anatech (no alcohol) on mouse bones with good results. It's possible that the alcohol is denaturing your antigens of interest. Is there a reason you need to use alcoholic formalin? Adam On Tue, Aug 16, 2011 at 7:45 AM, Liang, Frank wrote: > Hello, > > D

RE: [Histonet] IHC on Frozen Tissue

2011-08-16 Thread Sheila Fonner
Jan, Thanks, I really appreciate the help. This is my first attempt, so we'll see how it turns out. :) Sheila -Original Message- From: Jan Shivers [mailto:shive...@umn.edu] Sent: Tuesday, August 16, 2011 9:21 AM To: Sheila Fonner Subject: Re: [Histonet] IHC on Frozen Tissue Sheila,

RE: [Histonet] protocol for in situ RT_PCR

2011-08-16 Thread Liang, Frank
Hello, Does anyone have used alcoholic zinc-formalin to fix mouse tissues? We tried a few times with this fixative for immunohistochemical staining, it did not work, but when switched back to 4% paraformaldehyde, the immunostaining worked. We like alcoholic zinc-formalin as it gives better mo

RE: [Histonet] IHC on Frozen Tissue

2011-08-16 Thread Sheila Fonner
Rich, Isn't the cell conditioning (retrieval) mostly done for tissues that have been in formalin? If the tissue is fresh (frozen), it has not seen formalin. That is the reason I questioned the cell conditioning step. Thank you for your help. Sheila From: Richard Yeo [mailto:r..

RE: [Histonet] IHC on Frozen Tissue

2011-08-16 Thread Esparza, Sandra
I leave out the cell conditioning step as well. Sandra Sandra Esparza HT(ASCP)QIHC Lead Technologist Dell Children's Medical Center of Central Texas 512-324- x87061 sespa...@seton.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.

RE: [Histonet] IHC on Frozen Tissue

2011-08-16 Thread Sheila Fonner
Does anyone else leave out the cell conditioning step? -Original Message- From: Yahoo [mailto:kim.tourn...@yahoo.com] Sent: Tuesday, August 16, 2011 7:41 AM To: Sheila Fonner Subject: Re: [Histonet] IHC on Frozen Tissue I would leave out the pretreatment step as well i.e. Enzyme, CC1/CC

[Histonet] Re:

2011-08-16 Thread lynn13361
I want you to look throw this site! It�s the most interesting stuff I�ve seen last time!... http://listadoweb.com/com.page.php?jnohot=49ek4 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/

RE: [Histonet] IHC on Frozen Tissue

2011-08-16 Thread Sheila Fonner
Thank you. -Original Message- From: Mierow, Brett T. [mailto:brett.mie...@essentiahealth.org] Sent: Tuesday, August 16, 2011 7:02 AM To: Sheila Fonner Subject: RE: [Histonet] IHC on Frozen Tissue You are correct, leave out the depar. step. Brett Mierow, HT (ASCP) Histology specialist

[Histonet] IHC on Frozen Tissue

2011-08-16 Thread Sheila Fonner
Good morning everyone, I was wondering if anyone could help me with a protocol change for the Ventana Ultra. I have a pathologist that is requesting an HSVI, HSVll, and Zoster Virus on frozen tissue that was received for IF studies. I know it's not Monday, but it is early, and I was hoping for

Re: [Histonet] protocol for in situ RT_PCR

2011-08-16 Thread Louise Renton
hi could you add me on too pelase? On Mon, Aug 15, 2011 at 11:09 PM, Barone, Carol wrote: > Anyone got a hot protocol for In situ RT-PCR? Haven't done one in 5 > years (on my Perk and Elmer)...Know there must be some great new > reagents and kits out there...opinions? Send to cbar...@nemours.org