The un-coupled Naphthol salts (from the phosphatase reaction, dissolved in
the lipids of the tissue) degrade and cause N2 bubbles .
We solved this problem (in the 60ties!)with a post treatment after the
reaction:
After the reaction, rinse in dist water,
post fix in formalin 1:4 parts water (just
Sorry let me send that again with a link
*http://tinyurl.com/3uw5lcm
*I have no idea why we use this particular medium. *
*
A great book should leave you with many experiences, and slightly exhausted.
You should live several lives while reading it.
-William Styron
On Tue, Aug 16, 2011 at 9:40 P
We use Gel Mount
A great book should leave you with many experiences, and slightly exhausted.
You should live several lives while reading it.
-William Styron
On Tue, Aug 16, 2011 at 2:48 PM, Kevin Bennett wrote:
> All,
> Can anyone recommend a quality aqueous mounting media? I was using Aqua
I concur on this.
If my boss will let me, I can send you our elaborate protocol for H&E
staining, It involves many dishes and many washes. But your H&E will be
perfect.
I don't have the protocol with me right now,as I'm at home but tomorrow I
will ask about sending you the protocol. I don't see
Hey All,
I have been working on updating the look of our website and booth for
NSH. The graphic design people I am working with don't seem to be
getting the look I am going for. This gave me an idea. I would like to
challenge all of the crafty Histoneters to draw, paint or Photoshop (or
whate
Probe mount aqueous based mounting media from Innovex. My personal
favorite!!
Loralei Dewe
On Tue, Aug 16, 2011 at 12:05 PM, Montina Van Meter <
montina.vanme...@pbrc.edu> wrote:
> ProLong Gold (Invitrogen)
>
>
>
>
>
>
> Montina J. Van Meter, HT (ASCP)
> Lab Manager
> Dept. of Autonomic Neuros
ProLong Gold (Invitrogen)
Montina J. Van Meter, HT (ASCP)
Lab Manager
Dept. of Autonomic Neuroscience
Pennington Biomedical Research Center
6400 Perkins Rd.
Baton Rouge, LA 70791
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsou
We have been using an aqueous mounting media from Diagnostic Biosystems.
http://dbiosys.com/
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-boun...@lists.
Our protocol is to:
Use a positively charged slide
Fix in 10% formalin for 1 minute
Rinse in tap (a few dips)
Gill 3 for 1 minute
Rinse in tap (a few dips)
3-5 dips in bluing
Rinse in tap (a few dips)
Eosin 2-3 dips
100% ETOH (a few dips)
100% ETOH (a few dips)
Xylene (dip until clear)
Mount with
All,
Can anyone recommend a quality aqueous mounting media? I was using Aquatex
from EMD chemicals but it's been discontinued, tried Aqua-Mount from Thermo
but show air bubbles a few days after coverslipping.
Thanks,
Kevin Bennett HT
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http://stainsfile.info/StainsFile/dyes/26905.htm
This says 66...
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[
Hi,
I am wondering if anyone can help me out with some processing issues that our
lab has been having. We recently have been having reagent contamination issues
with alcohol and xylene, where our tissues are soft and cloudy after
processing. It seems that the reagent valves on the processor we
Details about Masson Trichrome Stain !
Biebrich Scarlet:
Biebrich scarlet 2.7 gm
Acid fuchsin 0.3 gm
Distilled water 300.0 ml
Glacial acetic acid 3.0 ml
I am not sure what the Biebrich Scarlet here is ? Acid Red 66 or acid Red 73 ?
Acid Red 73
http://www.sigmaaldrich.com/catalog/ProductDetai
Hello all,
I am considering purchasing a freezing unit through Thermo that can be used
with either isopentane of a new solution, HFE-7000 that is sold with the unit.
I need to be able to have frozen samples that allow high quality DNA and RNA
extracted. Does anyone have any experience with th
Hello everyone,
Thanks for the response regarding the Toxoplasma control. It's nice to
have such a supportive network available for these types of needs.
Margaret
Margaret G. Coppin, HT(ASCP)
Technical Supervisor--Immunohistochemistry
ARUP Laboratories
500 Chipeta Way
Salt Lake
Dear Colleagues:
I have just received the following e-mail from Leica Microsystems.
It is evident that I was wrong and that now the tissues in the Peloris
instrument go directly from the 2-propanol to the parafin wax and are not air
dried.
I just wanted to share this correction with you.
René J.
We do this frequently.
Fix slides in Methanol (45ml) + formaldehyde (5ml) for 10 minutes.
Wash several changes of tap water
Stain with Harris Hematoxylin for 3 minutes
Proceed as you would for paraffin sections
Get great staining.
I am sure if you are using a different Hematoxylin you could use
I've used 10% zinc buffered formalin from Anatech (no alcohol) on mouse
bones with good results. It's possible that the alcohol is denaturing your
antigens of interest. Is there a reason you need to use alcoholic formalin?
Adam
On Tue, Aug 16, 2011 at 7:45 AM, Liang, Frank wrote:
> Hello,
>
> D
Jan,
Thanks, I really appreciate the help. This is my first attempt, so we'll
see how it turns out. :)
Sheila
-Original Message-
From: Jan Shivers [mailto:shive...@umn.edu]
Sent: Tuesday, August 16, 2011 9:21 AM
To: Sheila Fonner
Subject: Re: [Histonet] IHC on Frozen Tissue
Sheila,
Hello,
Does anyone have used alcoholic zinc-formalin to fix mouse tissues? We tried a
few times with this fixative for immunohistochemical staining, it did not work,
but when switched back to 4% paraformaldehyde, the immunostaining worked. We
like alcoholic zinc-formalin as it gives better mo
Rich,
Isn't the cell conditioning (retrieval) mostly done for tissues that have
been in formalin? If the tissue is fresh (frozen), it has not seen
formalin. That is the reason I questioned the cell conditioning step.
Thank you for your help.
Sheila
From: Richard Yeo [mailto:r..
I leave out the cell conditioning step as well.
Sandra
Sandra Esparza HT(ASCP)QIHC
Lead Technologist
Dell Children's Medical Center of Central Texas
512-324- x87061
sespa...@seton.org
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.
Does anyone else leave out the cell conditioning step?
-Original Message-
From: Yahoo [mailto:kim.tourn...@yahoo.com]
Sent: Tuesday, August 16, 2011 7:41 AM
To: Sheila Fonner
Subject: Re: [Histonet] IHC on Frozen Tissue
I would leave out the pretreatment step as well i.e. Enzyme, CC1/CC
I want you to look throw this site! It�s the most interesting stuff I�ve
seen last time!... http://listadoweb.com/com.page.php?jnohot=49ek4
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Thank you.
-Original Message-
From: Mierow, Brett T. [mailto:brett.mie...@essentiahealth.org]
Sent: Tuesday, August 16, 2011 7:02 AM
To: Sheila Fonner
Subject: RE: [Histonet] IHC on Frozen Tissue
You are correct, leave out the depar. step.
Brett Mierow, HT (ASCP)
Histology specialist
Good morning everyone,
I was wondering if anyone could help me with a protocol change for the
Ventana Ultra. I have a pathologist that is requesting an HSVI, HSVll, and
Zoster Virus on frozen tissue that was received for IF studies. I know it's
not Monday, but it is early, and I was hoping for
hi could you add me on too pelase?
On Mon, Aug 15, 2011 at 11:09 PM, Barone, Carol wrote:
> Anyone got a hot protocol for In situ RT-PCR? Haven't done one in 5
> years (on my Perk and Elmer)...Know there must be some great new
> reagents and kits out there...opinions? Send to cbar...@nemours.org
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