Re: [Histonet] New York Licensure

They will need to take the HT and have the results sent to NYS for their license. At this time NYS does not recognize the HTL exam for licensure eligibility. Jen Campbell Sent from my iPhone On Jun 18, 2013, at 10:46 AM, "Mayer,Toysha N" wrote: > Good morning, > > This question is for those

Re: [Histonet] (no subject)

We use India ink and then use 3% acetic acid to affix it to the specimen. Works great on ellipse excisions as well as small slices. Jen Campbell On Wed, Mar 6, 2013 at 10:49 AM, Dermpath Lab wrote: > Hi! I was just wondering what different facilities use for red ink/dye. > Some of the new ones

Re: [Histonet] troubleshooting help

We have seen that if our paraffins were not properly rotated. On Mon, Feb 11, 2013 at 10:09 AM, Webb, Dorothy L < dorothy.l.w...@healthpartners.com> wrote: > I am having trouble with this scenario that I have walked into today. Our > Sakura VIP 5 tissue processor had about 150 cassettes on it an

Re: [Histonet] RE: Metal molds

We clean ours in the VIP every Friday. We recycle our xylene substitute from the VIP but you cannot recycle the cleaning alcohol. They are all "bright and shiny" for Monday morning! Jen Campbell On Wed, Oct 10, 2012 at 9:46 AM, Bartlett, Jeanine (CDC/OID/NCEZID) < j...@cdc.gov> wrote: > Does an

Re: [Histonet] Sakura glas coverslipper and mounting media types

ClearMount from American MasterTech. Speed and volume set to "3". We put our slides overnight in 60 degree oven prior to filing. On Thu, Sep 13, 2012 at 12:58 PM, Cathy Crumpton wrote: > For those with the Sakura Glas coverslippers, what type of mounting media > are you using in these? Sakura r

Re: [Histonet] Eosin

What clearing agent are you using? If it is a xylene substitute then you might want to rotate or change the alcohols after eosin after a few racks of slides. We use ProPar on our stainer and rotate our alcohols after eosin after every 3 racks that have gone through. On Thu, Aug 9, 2012 at 11:2

Re: [Histonet] Teabags

We use actual teabags that we purchase in bulk. We filter the contents of our specimen bottles but instead of filtering into the teabag we make a cone-shape and filter onto the teabag and then neatly fold it to fit in a cassette. We are a derm lab so some of the shave biopsies we receive are curled

Re: [Histonet] Embedding beads

The identifier(whatever is using to distinguish who did the task) is not embedded with the specimen. It is put in the top portion of the cassette. At least that is what we have always done here. On Mon, Jul 2, 2012 at 1:17 PM, Amber McKenzie < amber.mcken...@gastrodocs.net> wrote: > How do you

Re: [Histonet] Embedding beads

We use squares of colored construction paper. Each tech is assigned a color and they use those or their initials for all of the different jobs in the lab. There has to be accountability for every job. Jen Campbell On Mon, Jun 25, 2012 at 9:43 AM, Amber McKenzie < amber.mcken...@gastrodocs.net> w

Re: [Histonet] BLock alignment tool

American MasterTech carries it and I believe it was ~$200. Newcomer Supply had it too but I think the price was higher from them. On Thu, May 31, 2012 at 2:48 PM, Vickroy, Jim wrote: > Awhile back we got an advertisement for a block alignment tool that would > be helpful in recut cases. Can an

Re: [Histonet] paraffin melting in VIP

I agree Rene! I also believe Sakura recommends not putting paraffin flakes directly in the containers. On Fri, May 11, 2012 at 2:27 PM, Rene J Buesa wrote: > Regardless of the time it takes or of how many people do it, melting the > paraffin directly in the VIP should not be done because it ca

Re: [Histonet] Question about slides

Ward's Natural Science. On Mon, Mar 5, 2012 at 7:43 PM, Komal Gada wrote: > Hello Histo-netters, > > I'm looking for a source which sells slides for a Tissue Identification > class. Does anyone have any leads on where to go for something like this? > > Thanks, > Komal > _

Re: [Histonet] RE: Pro-Par

We do rotate our alcohols after eosin to minimize this problem. We recycle our alcohol from there so there is a cost savings. Every lab has their setup that works best for them while pleasing the pathologist(s) too. I love this forum because you can get informed opinions! On Tue, Feb 7, 2012 at

Re: [Histonet] Pro-Par

We have been using ProPar for 15 yrs now. We were so glad to find something that allowed us to become virtually xylene free. We still need xylene for some things but not nearly the quantity that we used to keep around. The folks at Anatech were so helpful in helping us get started. We also recycle

histonet@lists.utsouthwestern.edu

We use ProPar on our stainer. We rotate our 4 100% alcohols that are after eosin after every 3rd rack and we have stopped the leaching of eosin in our last ProPars. Not a big fan of exposing my staff to xylene unless I absolutely have to. Jen Campbell On Thu, Sep 15, 2011 at 9:44 AM, wrote: >

Re: [Histonet] harrasment for humble histotechs

I kicked ass on the HT practical! No way I was going to go through that hassle again. I am still puzzled as to why the powers that be got rid of it. It definitely tested how well you could follow directions too. On Sat, Aug 27, 2011 at 4:50 PM, histot...@imagesbyhopper.com < histot...@imagesbyho

Re: [Histonet] Slide Bright Question

We use ProPar and have found a way to survive the issue. Our stainer racks cause a lot of solution carryover so we rotate the alcohols after eosin after every third run. Once there is water in your final clearing containers they must be changed. Jen Campbell, HT(ASCP) On Fri, Jun 3, 2011 at 2:2

Re: [Histonet] Recycled Xylene

Very true because if you notice the label on a purchased bottle of xylene it says "Xylenes". Your recycled product should be pure xylene and thus a higher purity than what you started with. Our lab has been recycling since the mid '90's. We no longer process with xylene but we still have it in the

Re: [Histonet] IHC run print outs

We had our in-house IT person create a print-out for us. We are a private lab so we are not inspected by CAP. That being said NYSDOH requires much of the same QC paperwork so we make do with what we have. On Wed, May 11, 2011 at 11:09 AM, WILLIAM DESALVO wrote: > > To meet CAP requirements, we pr

Re: [Histonet] patient melanin pigment

We do a Melanin Bleach stain. On Wed, Apr 6, 2011 at 2:16 PM, Michele Carr wrote: > Hi, I was wondering if there were a way to remove a patients melanin > pigment on > a slide. Any helpful advice would be appreciated. > Thanks, > Michele Carr > Medical Laboratory Services > > > > > __

[Histonet] coverglass

I am looking for vendors for coverglass for an automated coverglasser. I want one where the coverglasses don't stick together. We are currently using Mercedes as our vendor. Any feedback out there for ThermoFisher? -- Jen Campbell Supervisor of Technical Services Muhlbauer Dermatopathology Labo

[Histonet] job opening

Histonetters, Private dermpath lab in central/western NY is looking for a fully licensed (in NYS) histotech for a full time position. Please contact me directly by email or phone. -- Jen Campbell Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory P: 585.586.5166 F: 585.586

[Histonet] Help with Biocare polymer detection kit!!

30 min, respectively, to 10 min each. I have tried cutting down my antigen retrieval time substantially as well. I'm not sure what else could be causing this. Any suggestions?? Thanks in advance, Jennifer Campbell ___ Histonet mailing

[Histonet] Difference between Protease and Proteinase-K?

n the differences between the two? Thanks, Jennifer Campbell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Has anyone used a biotin block in their antibody diluent?

vigorous may cause the unmasking of biotin, and its subsequent staining. I would like to know if anyone has had any luck using a biotin block in their diluent because I may try that as well. Thanks, Jennifer Campbell ___ Histonet mailing list

[Histonet] NY licensing

would they require me to complete a histology program? I have yet to find the chance to call them since they close at 4:45 ET and I am on the west coast. Thanks in advance, Jennifer Campbell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] recommendation for C-kit antibody

I'm looking for a C-kit antibody for IHC on FFPE K9 and Fel tissue. I've only found a couple of vendors that meet those requirements. Can anyone recommend one to me? Thanks, Jennifer Campbell ___ Histonet mailing lis

[Histonet] Moving across the country...

know of any possibilities out there? Thanks for any advice you may have. Jennifer Campbell VDx Veterinary Diagnostics Davis, CA phone: (530) 753-4285 fax: (530) 753-4286 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] nonspecific staining in liver

Hi All, I am having some problems with marked nonspecific staining in liver when running my CD3 and CD79a. I am using an avidin and biotin block, as well as a protein block. Any other suggestions as to what I could do to eliminate this? Thanks in advance, Jennifer Campbell

[Histonet] reagent alcohol in 50-55 gal drums

Hi All, Does anyone out there purchase their reagent alcohol in 50-55 gal drums? How much does this cost you, including the shipping & handling and any other costs? We are trying to determine whether this would be a good idea or not. Thank you, Jennifer Camp

[Histonet] Fluoro Jade B (or C) protocol

Hi All, Does anyone have an IHC protocol for Fluoro Jade B (or C)? I have read it can be adapted from FITC to IHC but I can't find a protocol. Thanks, Jennifer Campbell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Thank you everyone!

Thank you for all of those who were so helpful in suggesting reading material my HT exam! I appreciate all of your input and kind words of encouragement. Wish me luck! Jennifer Campbell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] studying for ASCP certification exam

study Guide for Histotechnology". Are there any other sources you would recommend? I have taken a look at the suggested reading list on the ASCP website but, there are quite a few books listed. Thanks in advance, Jennifer Campbell ___ H

[Histonet] special stains

I couldn't agree with you more, Liz! I am from a GLP lab as well and we have to undergo validation for every single new piece of equipment we acquire otherwise we are not considered GLP-compliant. Who is to say that your new stainer is going to stain slides in the same manner as your old stainer?

[Histonet] leaving IHC slides in wash buffer

Hi Everyone, I have yet another IHC-related question. So after antigen retrieval, and then allowing my slides too cool, I place them in wash buffer before loading them on to the autostainer. Would leaving the slides in the wash buffer in the fridge for an extended period of time (say, 1 week)

[Histonet] any info on Glutathione S-Transferase Pi (GST Pi)?

Hi All, Has anyone ever used this antibody in FFPE mouse or rat tissue? How easy of an antibody is it to work with? Other comments? We may be doing this for an upcoming project and I just wanted to know what I could be getting myself into. Thanks! Jen Campbell

[Histonet] Granzyme B and F4/80 immunostaining

Hi All, I'm currently trying to work up Granzyme B and F4/80 on FFPE mouse tissue. I seemed to get a bit of non-specific staining for both and was wondering if there is any way I can eliminate some of this. For Granzyme B I am using a rabbit polyclonal from Abcam. My protocol goes like th

[Histonet] Fite control slides

I am looking for a reputable vendor for Fite control slides. My pathologist would prefer they be "leprosy containing skin slides". I also need the vendor to provide a positvely stained slide with the procedure as well. Thank you in advance Jen Campbell _

[Histonet] rabbit-on-rodent HRP polymer--Ready-to-use?

Hi All, I had posted a question about the rabbit-on-rodent HRP polymer from Biocare yesterday and I was just wondering if anyone could tell me whether or not this is RTU or can be diluted. It doesn't specify anywhere on the package insert. Thanks, Jennifer

[Histonet] Rabbit-on-Rodent HRP polymer

Hi All, I recently purchased a rabbit-on-rodent HRP polymer from Biocare. I will be using this on mouse tissue, using a rabbit polyclonal CD3 as my primary. I just wanted to clarify a couple things about the protocol before I try it out tomorrow and was wondering if anyone could offer some

[Histonet] CD3 on mouse tissue

Hi Histonetters, I'm trying to work up CD3 on mouse tissue. I knew I would have to get a mouse-on-mouse kit since CD3 is a rabbit polyclonal but do you use some sort of rodent kit to eliminate background staining? My secondary antibody is part of a multilink kit from Biogenex and is capable o

[Histonet] Is anyone familiar with Granzyme B antibody?

I'm trying to find out some information on Granzyme B antibody for immunostaining of FFPE mouse tissue. Does anyone have any vendors they could recommend and/or protocols? Thanks, Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.

[Histonet] cuisinart pressure cooker

Hi all, Does anyone use the cuisinart pressure cooker (with digital display) for their antigen retrieval and what settings do you use? I just purchased one and am about to try it out but, it has a high and low pressure setting, where you are supposed to select the time to pressure cook, and th

[Histonet] GSTP antibody

Hi All, Is anyone out there familiar with GSTP antibody? Do you have any information such as, pretreatment, incubation time, vendors etc? Thanks, Jen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mail

[Histonet] destaining cyto slides for immuno

Hi All, How would you go about destaining a cyto slide and running a CKAE1/AE3? I was told that acid alcohol to remove the stain beforehand may not be necessary as heat retrieval may remove it adequately enough. Does anyone have any recommendations? Thanks, Jen

[Histonet] removing coverslip from immuno slides

I coverslipped some immuno slides using our Tissue-Tek coverslipper and I need to lift the coverslips from a few of the slides (used the wrong length). For histo slides, we typically soak the slides in acetone until the coverslipping film is easily removed. Is it ok to soak immuno slides in ace

[Histonet] decal solution

Does anyone know what the optimal pH for a nitric acid decal solution should be? Right now our's is reading below 1.0 when tested and we are having problems with our tissue being overdecalcified. Thank you in advance. Jennifer C. ___ Histonet mailing

[Histonet] Question about DAB waste

We just purchased a Dako Autostainer and are in the process of figuring out how to get rid of the haz waste that we will be generating. The machine separates the haz from non-haz, thankfully. I've been communicating with our waste facility and have been having some difficulty. They want to know

[Histonet] Toulidine blue stain

Hi everyone, I'm trying to find a protocol for a toulidine blue stain for paraffin sections. I've looked in different text books and online and can't seem to find anything helpful. Does anyone have a good protocol for this? I need to order the stain rather soon but, first I want to know how mu

[Histonet] pressure cookers for IHC

Hi everyone, Does anyone use a pressure cooker for heat induced epitope retrieval? I know there are a lot of fancy antigen retrieval units out there that you can buy specifically for IHC but, I know a lot of people just use a pressure cooker with the same success. Any specifics as to what type

[Histonet] Opinions on the Labvision 360 autostainer?

Hi everyone, Has anyone used the Labvision 360 autostainer? Any good or bad things about it? Is it suitable for IHC on animal tissues? Trying to sort out which stainer will work best for us and which is the most economical of course. I appreciate any feedback. Please no inquiries from sale

[Histonet] dako autostainers

We are looking into buying the Dako Autostainer Plus. I asked our rep if they still have any older models around to look into. He made it sound like there are older models but, the stainer itself has stayed the same, just the software has changed/improved. He said they don't even like selling the

[Histonet] In regards to a previous email I sent out..

ring. If phone calls of this matter could be avoided, I would greatly appreciate it. Thank you, Jennifer Campbell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Any opinions on these microtomes?

me by a sales associate but, I would like to hear if anyone has some firsthand experience with any of these and what his or her thoughts are. Thanks a bunch, Jennifer Campbell Vdx Veterinary Diagnostics Davis, CA ___ Histonet mailing list Histonet