You will need to make sure all pertinent SOPs and EOPs are followed, as well as
all safety guidelines/protocols. Just because it is not human tissue doesn't
mean that it can't have its share of nasties.
Joe Saby
Sent from Yahoo Mail on Android
On Thu, Apr 16, 2020 at 8:21 AM, Porter, Amy
Beth-
I am proud of you for stepping up and correcting the record.
This could not be an easy thing to do in such a public forum, but it also
essential.
I hope you have a wonderful holiday.
Joe Saby
On Wednesday, July 3, 2019, 2:33:16 PM EDT, O'Neil, Beth via Histonet
wrote:
Last week
The more important question is who the h*ll is Kelly Jordan.
Terri has established her competency and reputation in NSH and the Histonet for
probably over 20 years.
You have done your company a great disservice.
People will remember you, probably not as you would wish.
Joe Saby, retired
On Tues
Gudrun-
Assuming this is a new issue with a recent reagent change, I suspect that the
alcohol used for the clean cycle was not 100% (maybe 95%?).
The ability of your alcohol to hold the paraffin in solution is lost as the
percentage of water increases.
I also expect your solvent was mostly satur
Talk with the people at EXAKT technologies. They have plastice specially
designed just for samples like that.
Joe
On Friday, June 22, 2018, 1:47:38 PM EDT, Terri Braud via Histonet
wrote:
Just an idea. Why not use SEM? It seems like it would be so much easier.
Terri L. Braud, HT(A
Eileen-
I think it would depend on what specific procedures both of them are signed off
on.And it would be on specific procedures.Or things are very strange in that
area of Histoland.
Joe Saby
From: Eileen Akemi Allison via Histonet
To: Histonet
Sent: Wednesday, November 1, 2017 8:
Andrea-
I believe your tissue was not fully fixed. Many labs think they know more than
they do, so itis always a good idea to pin down exactly what they did to fix
the tissue. I am guessing they left thick
blobs of tissue in an inadequate volume of formalin and expected that to do the
trick.
We had this happen when the individual changing the processor reversed order
of the alcohols, placing the newest first and the oldest last. Hope your
answer
is as simple, even if it is embarrassing.
Joe Saby
From: "Abbott, Tanya via Histonet"
To: "histonet@lists.utsouthwestern.edu"
Heather-
I do not know why, but to properly process guinea pig tissues you need a much
more rigorous program than what would work for mice. It is very easy to over
process mouse tissue. Even rat tissue needs more processing. Guinea pig
tissue need a program designed for processing larger ani
dshos http://bran-denschools.org/hui/jdul/jifh/pbiaq.htm
Joseph Saby
mhphq
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Wherever I have worked, trimmed cassettes have always gone into 10% NBF unless
there were specific requirement by protocol or for a special procedure.
Joe Saby
From: Kate
To: "histonet@lists.utsouthwestern.edu"
Sent: Thursday, January 12, 2012 5:16 PM
Subject: [Histonet] Trimming question
H
I would suggest that you look inthe local yellow pages for people who work with
plexiglass. This is a re;atively inexpensive medium. You can design what you
need and have your draft shields built to order.
Joe Saby
NAMSA
From: "Rathborne, Toni"
To: 'Keri Colwell' ;
"histonet@lists.utsouthw
We use the expiration date on the formalin as the use by date. By the time the
solution would be out of date, the tissues should be very well fixed, so the
formalin becomes just a holding solution.
Joe Saby
NAMSA
From: "amitapan...@torrentpharma.com"
To: hi
Mahesh-
If you have access to a sonicator, you can etch the slides by first soaking
them
in 1% formic acid in the sonicator for 5-10 minutes, then after a brief water
rinse soak them in 50% ethanol for another 5-10 minutes in the sonicator.
You may have to work with the staining times, but you
Kathy-
What do these cracks look like? Are they arranged in a parallel manner? Or do
they have the appearance of the cracks seen in dry mud?
Parallel aligned cracks are often found in overprocessed small biospies. These
small samples become hard and brittle. The impact of the tissue on the
Perhaps the best and least expensive tissue save bag sealer I have found can be
purchased from ULINE. There are benchtop models that easily fit in a hood,
there are floor models. The benchtop model we use cost less than $200 USD,
easily fits in a hood, and has been totally trouble free for 2 y
I knnow you can get vacuoles in rodent brains when processing them over the
weekend with a hold station of 60-70% ethanol. Other than that I cannot say.
Joe Saby, BA HT
From: Pathology
To: histonet@lists.utsouthwestern.edu
Sent: Mon, August 30, 2010 7:07:22 A
Mike-
Although Sakura is no longer making parts for the VIP 2000 units, there are
thousands still in use around the country. Most companies that service these
units have been taking them in trade as people upgrade their equipment, then
using these units to provide parts for units in trouble al
Dorothy-
If your bones are indeed large, 24 hours in 10% NBF will not be sufficient for
fixation. If the fixation isn't sufficient, none of the processes following
that will be as effective as they might be. Even after trimming, I would
recommend fixing an additional 24-48 hours for every mm
What you are describing might be microchatter. These will be sharp parallel
lines/cracks that run parallel to the knife edge and are only visible under the
microscope.
The usuall cause is a combination of overprocessing and rough facing that is
too
aggressive and/or with too dull a blade. Ov
Sarah-
There is another possibility. If you use formalin in the first station, you
should rinse out the first 2 containers when changing the processor and fill
them with warm water. You can then perform a warm water flush. This is
setting
up a program with 1 minute in each of the first 2 st
Brandi-
Having gone through and even designed lab renovations, my advice is:
Have a different hood for each function if possible. Renovations cannot see
into the future to know what technologies you might need later. An extra hood
now can very easily seem like too few later. Also, many funct
Tahseen-
I work in the world of animal/research histology. I have spent many years
working with rodent and nonrodent eyes, developing my skills and knowledge.
Different fixatives work well with different parts of the eye. 10% NBF might
wotk well for cornea, bulbar and palpebral conjunctiva.
Sam-
Are you storing sectioned slides?
What we do is to deparaffinize and coverslip sectioned but unstained slides. I
haven' had to go back, but I believe they will store that way a long time.
Joe Saby, BA HT
From: "Perry, Samuel"
To: histonet@lists.utsout
Sara-
I just recently had a box of sticky blades. In fact, there was no way to pry
one loose.
However, some kind soul on the Histonet had posted that a few drops of
microtome (or sewing machine) oil will loosen the blades when stuck like that.
The effect may not be instantaneous, but it does
Brett-
Most wrinkles in decalcified bone sections come from stretching of the
decalified bone that occurs during the sectioning process. I would suggest a
rather simple solution. Allowing the sections to flatten on the waterbath
might take longer or a higher temperature. If paraffin surround
Brandi-
The flush should be with warm water (you do not need hot), and should be for
the fixative station(s) and the first alcohol. You only need 1 minute in each,
just enough to get the solution to pump in.
Joe Saby, BA HT
From: Anthony Reilly
To: Brandi
All-
From previous work with rat perfusions, the flow rate was about 10 ml/minute.
If I had to guess, the equivalent flow rate for a mouse would be closer to 1-3
mls/10 minutes. If you go 10 ml/minute, you will definitely cause blowout
artefacts.
Joe Saby, BA HT
_
Running tap water for 10 minutes should do the trick.
Joe Saby
From: Cynthia Pyse
To: cscam...@uci.edu; HistoNet
Sent: Tue, March 16, 2010 10:52:20 AM
Subject: RE: [Histonet] Removing Yellow color from slides refixed in Bouin's
solution
I just place the sli
Betsy-
When staining for proteoglycans, a stain usually associated with Alcian Blue
would be PAS. Stain with Alcian Blue first, then perform the PAS. A light
hematoxylin usually completes the stain.
Joe Saby, BA HT
From: "Molinari, Betsy"
To: histonet@lis
Robert-
The artifact you describe is almost always due to varying combinations of two
issues:
1) overprocessing the biopsies, making them tough, and
2) too agressive facing of the blocks.
Thick facing sections cause cracks deep in the tough tissue.
I have worked through these issues in
I have a full time position open for a histotech at NAMSA, located just south
and east of Toledo in Northwood, Ohio. This is a full time days position, and
requires skills in embedding, sectioning, staining, etc. Must be willing to
work on animal tissues. We work on many interesting specimens
Fellow Histonetters-
I could use some help.
I have a request to use a stain for sympathetic nerve fibers called Eager's
that can be combined with Luxol Fast Blue / Crsyl Echt Violet.
I know many of you would recommend GFAP or S100, and you would be absolutely
correct. Unfortunately, that is n
Mercedes Medical has a "Quick Dipp" stain that I have heard is equivalent, b ut
much less expensive. Or you cxan always use Wright stain or Methylene Blue.
Joe Saby, BA HT
From: "Feher, Stephen"
To: mlbuk...@ucalgary.ca; histonet@lists.utsouthwestern.edu
Sen
Although I really like MOPEK, another source for the East Coast would be TBJ.
From: Golden State Acrylic Designs
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, July 16, 2009 9:34:56 AM
Subject: [Histonet] Biological hood with grossing station
Is the a s
I agree that the pale liver is a great sign. However, if the lungs fill up
fluid comes out the nostrels or mouth, then the needle is probably inserted too
far and has gone into the pulmonary vein.
I hope this helps!
Joe
From: Merced M Leiker
To: "Thach, Dz
1% Nitric acid in 70% ethanbol.
Joe Saby
From: "thisis...@aol.com"
To: histonet@lists.utsouthwestern.edu
Sent: Friday, June 19, 2009 5:01:48 PM
Subject: [Histonet] Acid Alcohol
I have lost the recipe for Acid Alcohol (used to decolorize H&E's).? Can
someone
Hello to Histoland-
I have a question concerning staining controls.
I am currently working for a GLP/GMP lab. Their use of staining controls
requires the purchase of said controls from sources such as Histology Controls
Systems (TM) where the manufacturer certifies that these controls are effe
I can personally attest that Ford must have been having a VERY bad day indeed.
He has supplied excellent information to this list on many occasions to many
people over many years, and until that listing has always been very courteous.
We've all had bad days. I would suggest cutting him some sl
Tracy-
Where I used to work (at a place that shall remain nameless), we always kept
our tissue being embedded in hot paraffin in the holding chamber. Most of my
work has been with animal tissues.
Where I work now, we don't. And I do bellieve you are right. If the tissues
remain in hot paraf
All-
I did say the unit was a real workhorse! In all honesty, we did use ours hard
for well over 10 years before we moved on the the newer VIP 2000s and VIP 3000s.
Unfortunately, we remember our last experiences better than the early ones. We
had many good years with our VIP 1000s. It wasn't
I worked with a VIP 1000 well over 20 years ago. This is a dinosaur!
Issues that will come to haunt you:
The retort lid will warp over time (if it isn't already warped). Minor
overtensioning of the clamps cause this. Many years ago we were told that new
lids were no longer available.
All-
If you are planning on looking at the bone marrow or are going to be evaluating
nuclei in the bone, then I would heartily recommend either using 5% formic
acid, or, preferably, a buffered formic acid solution. The buffer can either
be sodium formate or sodium citrate (set to a pH of ~2.2-
Lee and Peggy-
We section these eyes all the time with the plastic in place.
I would certainly suggest a gluaraldehyde fixative. Straight NBF is a very
poor fixative for the many tissues found in eyes, especially the retina. If
you contact me directly, we can talk off line. Eyes have held
I had a little problem once with Schiffs.
I was making up a new batch (I believe about 2 liters) and had just finished
boiling the water. I was in a hurry (often the culprit in disasterous lab
events) and forgat that impure solvents have a lower boiuling point. When I
added the basic fuchsin,
While cutting myh teeth in research many years ago, I performed rat vaginal
smears and did indeed use the PAP stain. It worked very nicely.
From: "Della Speranza, Vinnie" <[EMAIL PROTECTED]>
To: Helen E Johnson <[EMAIL PROTECTED]>; "histonet@lists.utsouthweste
Jenee-
I have almost always used 10% NBF for brain fixation. We're talking rats,
mice, guinea pigs, hamsters, pigs, dogs, primates, etc.
The only exception to that was when we were having an issue with the brains
settling to the bottom of the fixation container and developing flat spots
where
Jennifer-
Be of good cheer!
I saw them at the NSH convention.
Now if I could only remember where...
Joe
- Original Message
From: "[EMAIL PROTECTED]" <[EMAIL PROTECTED]>
To: Histonet@lists.utsouthwestern.edu
Sent: Thursday, September 18, 2008 3:47:34 PM
Subject: [Histonet] Casset
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