I have been thinking of unsubscribing to Histonet, but then something like
this comes along
and I start to rethink my stance.
I've never been a histotech, but spent 33 glorious years listening to Peggy
and others talk about it.
Some went over my head, but as time went by, less and less.
As
It is my sad duty to tell you that Peggy Wenk passed away peacefully
Saturday morning. Her husband and companion of 37 years and her
sister (from Bend,OR) were there.
As you know Peggy had a large influence in the world of Histology.
She also put herself into her church serving over the years
This is from the Theory and Practice of HIstotechnology, 2nd edition, 190.
Dezna C. Sheehan and Barbara B. Hrapchak. The reference for their procedure
is the article you mentioned.
FIXATIVE:
Mercuric formalin preferred, but 10% NBF can be used
SOLUTIONS:
Mayer Hemaxylin
Phloxine stain
- 1
I think this is mostly a safety issue, and suggest NOT allowing any amount
of formalin in OR/surgery rooms.
1. Training:
Doesn't matter how much or how little formalin is in the room. If it is
being used in a room, then everyone using it MUST receive yearly training on
formaldehyde and on
kinds of complicated storage regs
as to amount of formalin, size of room, ventilation rate through room, etc.
It would just be so much easier if NO formalin was allowed in the OR rooms.
Peggy A. Wenk, HTL(ASCP)
-Original Message-
From: Lee Peggy Wenk
Sent: Friday, June 13, 2014 7:44 AM
Try changing the angle of the knife blade, so that the clearance angle
(angle between the knife blade and the block face) is larger. In other
words, tip the top of the blade towards the block more. If there are numbers
on the side of the knife holder, you want to move it to a larger number
You didn't mention what your current cutting speed is, nor what speed they
want you to be at.
Ask your supervisor to set up goals for you to meet, with time frames. Each
week, work on getting faster, not fast today, just faster each week.
At the School where I taught, we had goals set up
You need to meet with the architect, and engineer, and the fire marshal. And
they need to be knowledgeable about the chemicals, etc. that will be
stored/used in the lab area, and any rules/regulations in your country/area
that relate to building labs.
The laws have changed in many locations,
We always bought ours from Dorn and Hart, out of Chicago, Illinois.
http://www.dornandhart.com/
Peggy Wenk, HTL(ASCP)SLS
-Original Message-
From: idimi...@mun.ca
Sent: Wednesday, April 02, 2014 8:24 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] suppliers of reusable
One of my favorite Cary Grant odd ball comedies - Arsenic and Old Lace. He's
the only person I know of who can pull a triple look. If you have never seen
this comedy - rent it or down load it or whatever. But be glad Raymond
Massey isn't your brother (looking like Boris Karloff), and Peter
Posting this for a friend, so please reply to him, and not to me.
Part-time ASCP certified histotech needed in private derm/GI lab in New Jersey.
Day shift
Cut 40-50 blocks/day
Typical special stains include Steiner, Alcian Blue-PAS, and GMS
Contact John Howard at 973-650-4038.
Peggy A. Wenk,
Just wanted to remind people that NSH has $30,000 in scholarships and awards
available to help people attend NSH educational symposiums, participate in NSH
teleconferences, go to college, get histology/IHC/Mol path training at another
institution, help NAACLS students, etc.
Deadline for most
August 23 - 27, NSH Symposium in Austin, TX.
NSH is still working on the program, but here's their website - so check
back here often:
http://www.histoconvention.org/index.cfm
Peggy A. Wenk, HTL(ASCP)SLS
-Original Message-
From: Vanessa Perez
Sent: Thursday, February 20, 2014 10:15
to be used for what?
- Lipids - ORO, Sudan black B?
- Immunofluorescence - which dye?
Different needs, different requirements.
Peggy A. Wenk, HTL(ASCP)SLS
-Original Message-
From: Jennifer MacDonald
Sent: Wednesday, February 19, 2014 12:27 PM
To: histonet@lists.utsouthwestern.edu
The following companies sell refurbished used histology equipment. They
might have parts that they sell - I don’t know as I never asked them if they
sell parts, but it's a place to start. (I've worked with both companies,
buying used equipment for the School.)
IMEB
http://www.imebinc.com/
The only soaking artifacts that I can think of would be caused by:
- soaking too long in water (minutes instead of a few seconds)
- soaking under-processed tissue in water
In both cases, the tissue is supposed to be protected by the wax, and if
it is not (under-processed), or if the faced block
Peggy Wenk
Sent: Monday, January 06, 2014 8:07 AM
To: Deanna Leslie ; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Soaking artifact
The only soaking artifacts that I can think of would be caused by:
- soaking too long in water (minutes instead of a few seconds)
- soaking under-processed
the processor more efficiently,
which will make the rest of the work more efficient. And increase
productivity and make turn around time faster.
Peggy A. Wenk, HTL(ASCP)
-Original Message-
From: Podawiltz, Thomas
Sent: Monday, January 06, 2014 12:04 PM
To: Lee Peggy Wenk ; Deanna Leslie
Is the -80 degrees really a correct temp? We freeze our muscles around -150
to -160 Degrees C.
Peggy A. Wenk, HTL(ASCP)SLS
-Original Message-
From: Ignacio Ruz Caracuel
Sent: Thursday, January 02, 2014 7:27 AM
To: Peter Petro ; histonet@lists.utsouthwestern.edu
Subject: Re:
For Schools in the British Isles:
http://www.nhshistopathology.net/
Click on Schools.
Peggy A. Wenk, HTL(ASCP)SLS
-Original Message-
From: Leila Etemadi
Sent: Thursday, January 02, 2014 12:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Histology program/school?
If anyone wants to find out where the HT or HTL NAACLS-accredited programs
are in the US, go to
www.naacls.org
on the left, click on Find a Program
Then you can click on Program Type and click on either HT or HTL.
If you want just one state, like Georgia, you can click on that. Or don't
click
All ASCP tests (HT/HTL, HT/MLT, CT, PB, QIHC, QLS, etc.) are all built the
same.
All are multiple choice questions, with 4 choices, one of which is the most
correct. (I won't say only 1 is correct, but if 1 is always correct or
almost always correct except in weird circumstances, and another
I took the exam when it was first offered, when it was a Specialist exam
with 100 questions. It's now a Qualification exam with 50 questions. But I
don't think it has changed that much, just fewer questions.
If you haven't been to the ASCP website on the Safety Exam, do that first.
Just for fun. This year’s top 10 Olympus Bioscapes digital imaging competition.
Most through microscopes.
http://www.wired.com/wiredscience/2013/12/olympus-bioscapes-microscope-photography/#slideid-395121
Peggy A. Wenk, HTL(ASCP)SLS
___
Histonet
If not interested in webinars designed for supervisors and
trainers/instructors, please delete.
NSH has developed a new set of webinars for supervisors and instructors. 11 in
total in 2014 – 3 on management, 3 on education/training, 5 on quality.
The webinars are $35 per person if ordered
Couple of studies that I know of.
One was sponsored by NSH in the mid-1980's. KH Kilburn came to several NSH
Symposiums, and did different tests on people who volunteered to
participate. Published findings in the late 1980's that said that histotechs
had lower pulmonary function than average
Sad news. I received an email over the weekend from a histotech, that Chris van
der Loos died on Nov. 26, 2013. I was able to talk with the NSH office today,
and got it confirmed. There is information about Chris on the NSH webpage as of
later this morning.
Just a thought - have you tried changing the angle of the blade?
Each different type of blade from different vendors need a different angle
for microtoming.
Each different type of microtome from different vendors need a different
angle for microtoming.
I know these two facts, as I have
I'm going to try to take a stab at this, but I don't know specifically about
the picrosirius red stain. I'm going to talk in general about stains and
fixation (or lack thereof in the frozen section (FS)), and the relationship
between fixation and staining. I'm going to start out very simple,
Promotion about NSH teleconferences for next year – if not interested, delete
now.
FYI – the 2014 NSH webinar schedule is now available, and so is signing up.
http://www.nsh.org/content/2014-nsh-laboratory-webinar-series-registration-now-open
If this link doesn’t work, go to NSH webpage
Electron Microscope.
Even if you did a periodic acid-methenamine silver stain (PASM, Jones),
which in my opinion is the best histology stain, since it is a silver stain,
you can get different thicknesses of basement membrane (bm) by leaving it in
longer or shorter than is optimal for that
If you are a CAP accredited lab, CAP says that the cryostat must be
defrosted and disinfectant decontaminated at regular intervals with a TB
disinfectant.
- - -
ANP.23410 Cryostat Decontamination Phase II
There is a documented procedure for the routine decontamination of the
cryostat at
Make your own.
Take some fresh lung (slightly edematous is better, if you can get it). Cut
into 2x2 mm cubes (or largest 3x3 mm cubes).
Contact Microbiology, and have them make a broth with a non-pathogenic AFB
in a large tube (e.g., plastic centrifuge tube). Put lung cubes in broth.
1. Convention booklet - At the convention, every attendee was given a
spiral bound booklet, with thick glossy pages. The last tab is the Speaker
Directory with Hospital/lab info, city/state, email addresses.
2. NSH Member Directory - go to NSH webpage (www.nsh.org ), go to Member
Directory
Personally, I think it's a is a wrong answer, and that you are correct
that b is a better answer. My students and I have found a couple of other
questions that we thought had the wrong answer indicated in the study set.
Peggy A. Wenk, HTL(ASCP)SLS
-Original Message-
From: Stephenson,
I just attended Jan Minshew's workshop on cryostats at the NSH Symposium in
Providence, RI, and she brought up something I had never thought of that
causes thick and thin.
If the handle that tightens the blade in the blade holder is over-tightened,
the blade will become bowed, and that will
going on. And then mark the question for a re-write next year. And
that doesn't include me marking the wrong answer on my master sheet. It
happens!
Peggy A. Wenk, HTL(ASCP)SLS
-Original Message-
From: Watson, Linda
Sent: Thursday, October 03, 2013 9:40 AM
To: Lee Peggy Wenk
For those going to the NSH Symposium, PLEASE unsubscribe, rather than having
everyone on the email list read your return message of “I am out of the office”
for every single Histonet email sent to you.
Reminder on how:
- Go to the bottom on any Histonet email
- Click on the internet link (the
Just a comment on the comment about there being not enough HT/HTL programs
in the US. Agreed. There are 38 HT and 7 HTL, for a total of 45. Compare
that with 223 MLS/MT and 233 MLT, for a total of 456, and we histotechs have
1/10 the number of programs as med techs.
If you are interested in
I'm going to wade in, not as someone who has posted numerous times on how to
unsubscribe, but as someone assessing it from a risk assessment
evaluation.
If there is a lab task that is consistently being done wrong, by many
different people, it is usually NOT the fault of the people. It is
On Sunday, Sept. 22, from 8 am - 9:30 am at the NSH Symposium in Providence,
RI, Beth Cox, HTL/SCT(ASCP)QIHC is presenting a workshop on Work and Play
Across the USA - A Guide to Being a Traveling Tech.
http://www.histoconvention.org/
Click on Schedule
Peggy A. Wenk, HTL(ASCP)SLS
On Wednesday (yesterday), there was a CAP teleconference on the
to-be-updated (to be posted end of July 2013) AP checklist.
Someone asked a question about this, mentioning that someone from CAP had
said competency assessment does not apply to histology.
The reply from the presenter and the
If you are going to vacation/holiday, please unsubscribe from Histonet. We
don’t want a zillion messages saying “I am out of the office.”
If you no longer want to receive Histonet emails because it isn’t your cup of
tea, then please follow these directions on how to unsubscribe:
- Go to the
What exactly is wrong with the fatty specimens?
If the nuclei look smudgy, with no nuclear detail, then it has not been
fixed long enough.
If the fat is still in the tissue and you cannot section it on a microtome,
then the tissue has not had enough time during processing, especially length
The poster reimbursement has been addressed. So I'll talk about workshop
presenters. It's not a free trip, but some of it is paid. For presenting 1
workshop, you are reimbursed:
- air fare or cost of driving, whichever is least
- parking at airport (if flying) or at hotel (if driving)
- 2
What happens if you skip the ammonium hydroxide?
Peggy Wenk
-Original Message-
From: Molinari, Betsy
Sent: Wednesday, May 29, 2013 7:45 AM
To: Lee Peggy Wenk ; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] another Movats question (paraffin)
Hi Peggy,
No, this is a batch I
I don't suppose you want to hear this, but several people are working
extra jobs.
Lee Wenk (not Peggy).
-Original Message-
From: Sarah Dysart
Sent: Wednesday, May 29, 2013 5:05 PM
To: Rene J Buesa ; joelle weaver ; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Extra Money
CAP does not put any number as to how much CE is required for working techs.
Just that there is a CE program (54200), and that there is a record of CE in
the personnel records (54400, #6).
GEN.54200 Continuing Education Phase I
There is a functional continuing clinical laboratory education
For everyone needing to unsubscribe over the summer months while on
vacation, or just because . . . keep this email handy.
Go to the bottom of any email.
The last line is an internet address - starts with http://has the word
mailman in it.
Click on the link.
Scroll to the bottom of the
Some time ago, someone asked what all the reagents in the Holzer stain were
for, and John Kiernan made some guess (see in quotes below for his response
to the PMA). If John KIernan is having to make educated guesses, the rest of
us probably have NO IDEA. Added to that, I don't know anyone who
Cresyl Echt Violet (CEV) is the pre-WWI name. After factories in Germany
were bombed, the formulation for this stain was lost (that's the story I
heard in the 70's).
Closest companies since then have made is cresyl violet acetate (CVA),
though for many decades, continued to call it CEV. Only
Definitely make it explosion-proof.
See the following article by Robert Skinner in Sakura's Histo-Logic.
http://www.sakura-americas.com/histologic/pdf/03_may.pdf
He had an explosion in a freezer, when a researcher tried to store 50 mL
(about $2.00) worth of isopentane in a regular freezer, and
Congo red is the gold standard for amyloid staining. It is the most
sensitive of the amyloid stain, at about 97%. However, sometimes the Congo
red will not stain the amyloid protein, such as when the amyloid is a large,
very old deposit. In that case, more and more amyloid is being crammed into
Go to the bottom of any email from Histonet.
Under the header “Histonet Subscribers” – go to the 2nd non-italics line that
starts “To unsubscribe from Histonet . . . “
- type in your email address where you are receiving the listserv emails
- click on “unsubscribe”
- follow rest of directions
If you are looking for glycogen - so a PAS. More sensitive and a deeper
magenta color than the Best Carmine.
Curious, what is without water?
- The Frozen section? But there's water in the cells, which is what is
freezing.
- The stain?? But most stains are made in water (aqueous
We always used a 2% Alizarin red S (never tried it with 1%. I'm guessing
that you would just have to leave it in longer.)
We pH it to 4.1-4.3 with 0.5% ammonium hydroxide. I don't remember what the
pH is when we begin, but note, we're adding a BASE to bring the pH up to
~4.2. So alizarin red
Just to let everyone know - the Michigan Society of Histotechnologists have
put together a Study Guide/Workbook, geared to help those studying for the
QIHC. It is a workbook, where you have to look up the information in books,
and write it in the booklet. There are no answers provided (it's not
Can you surface decal?
After facing the block on the microtome, getting a full face, pour some
decalcification fluid in the lid of a coplin jar. Place the block faced side
down in the decal solution, for about 30 minutes.
Rinse the acid off the block with some cool water (don't want acid
Getting back a little late (been out of state).
If this is Acanthamoeba, then we do a PASH and/or a GMS. These are cysts,
and show up nicely with both. The cornea can be PAS positive also, so the
Acanthamoeba are a darker pink against a lighter pink, while the GMS is
gray/black against the
(Quoted material taken from various ASCP BOC (Board of Certification)
webpages.)
FIRST: make certain they meet the ASCP HT criteria. If it they are truly
doing the OJT route:
Route 2: At least 60 semester hours (90 quarter hours) of academic credit
from a regionally accredited
-4300
ext. 164
HTL Barry University
11300 Northeast Second Avenue
Miami Shores, FL 33161-6695 Dr. Gerhild Packert
PhD
(305) 899-3220
Peggy A. Wenk, HTL(ASCP)
Beaumont Hospital
Royal Oak, MI 48073
Lee Peggy Wenk
-Original Message-
From: Bodden, Cheryl
Sent: Tuesday, January 08
Towards the bottom of the MSH webpage on study guides available through MSH,
is a link for the application form.
www.mihisto.org
click on Education
click on Study Guides
Peggy Wenk
-Original Message-
From: Ellen Yee
Sent: Monday, December 10, 2012 6:26 PM
To:
I think the key phrase was that the pathologists expected a small amount of
cells to stain in the esophagus biopsy.
If your control has a lot of acid mucopolysaccharides (AMP) in it (such as
using a normal intestine, or lung with bronchus), then 10 minutes in Alcian
blue would definitely show
Where most people get confused is theBiological Hazard. They think - this
chemical would hurt a human being, it would damage someone's skin if
splashed on it, it would injure someone's lungs if inhaled, it could cause
cancer with long exposure, etc. Since it's hurting a person, it must be a
If you want to unsubscribe permanently , OR
If you are going away for Thanksgiving and are setting your computer for “I’m
not here” (which all the Histonetters do not need to know), please unsubscribe.
Go to the bottom of any Histonet email, from the computer you are receiving the
Histonet
NSH has a jobs board - jobs available, and a place for histotechs to post
their information. Start there.
http://www.jobtarget.com/home/home.cfm?site_id=8282
Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073
-Original Message-
From: Ali A Krasht
Sent: Thursday,
Email has been down for several days, so if someone has already answered
this, sorry.
If anyone wants to donate used (in working order) equipment to a
NAACLS-accredited histotech program, it would be appreciated. Also any
supplies that you are no longer using (dry dye powdered because you
Talk with Jack Ratliff, Chair of the NSH Hard Tissue Committee.
Jack L. Ratliff
615-236-4901
ratliffj...@gmail.com
The answer is Yes, histologic sections can be made, but need plastic resins
(methyl methracylate or glycol methacrylate or something similar) and
special microtomes and knives.
If you are going to NSH, please UNSUBSCRIBE from HistoNet, rather than having
all of us get “I’m away from the office” notices on every HistoNet email sent
to you.
Go to the bottom of any HistoNet email, such as this one.
- Click on the bottom line, which starts out
For most histology demonstration of iron or copper, we are not doing a
quantitative analysis (exactly how much is in the tissue). We are usually
just demonstrating yes there is iron or copper, or no there is not. In the
case of hemosiderosis, hemochromatosis, or Wilson's disease, we might be
From CDC - not quite a lab, but in Jan. 2002, this company was melting
down the plastic from around capacitors, to regain the metals inside, by
putting the capacitors in a heavy metal pot with acid, and leaving it
overnight. The next day, the person went to remove the metal lid from the
metal
Looking for a small slide dryer, used preferably, for our School of
Histotechnology. Not a lot of counter space. Only 6 students, so don’t have a
lot of racks at any given time.
We have an old Shandon-Lipshaw rectangle metal “box”slide dryer right now, but
the insulation is going on it, so we
In the past, there were 3 routes (in short version):
- minimum associate degree (or 60 credit hours) with 12 credit hours of
biology and chemistry combined with 1 year on-the-job-training (OJT)
- high school diploma plus 2 years OJT
- completion of a NAACLS accredited histotechnology program
Drop of hematoxylin on the tissue, when put on the paper in the grossing
area. Use a syringe. Only a SMALL drop. Too much means there's extra blue
all over the paper, making it hard to see the blue tissue.
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073
The opinions
Hint when using these - do NOT try to fold them up into a nice looking
square. Once processed and in paraffin, it is very difficult to find the
edge, to try to open back up.
Fold into a not nice to look at, off-set square that is slightly crumpled.
Much easier to find the edge.
Peggy A.
From the computer that you receive your Histonet:
Go to the bottom of any Histonet email.
click on the link that end in listinfo/histonet
Scroll to the bottom of the page
Follow the directions to unsubscribe
Keep this email, if you plan to re-subscribe after returning from a vacation
or
Most of the state symposiums are in the spring, since the NSH symposium is
in the fall. I just checked the NSH meetings calendar, and I don't see any
state meetings for the next several months.
Any chance you can go to the NSH Symposium in Vancouver, Canada (which is
Region IX). Sept. 29-Oct.
Note: The exact wording under HT eligibility is:
. . . AND one year full time acceptable experience in a histopathology
(clinical, veterinary, industry or research) laboratory in the U.S., Canada
or an accredited laboratory* within the last ten years.
Very important to note the One year full
Tim, etal:
This is easily understood: focusing and setup work the same on binoculars,
one eyepiece is focused with the main focusing system, the other is used to
match
focusing with both eyes. First focus the scope (binoculars or microscope)
thru
the simple (non focusing) eyepiece, then use
Tim,
You are welcome, but I'm not Peggy. I'm Lee, her husband.
I'm very familiar with binoculars, so I only assumed that they are the same
as microscopes.
Lee Wenk
-Original Message-
From: Morken, Timothy
Sent: Tuesday, July 10, 2012 2:49 PM
To: Lee Peggy Wenk ; Histonet
Subject
Usually not.
Fixation cross-links the proteins, which can mask the epitope (=antibody
binding site of the antigen). So antigen retrieval breaks the fixative
cross-links, exposing the epitope.
If there's no fixation, there's no cross-links, so the epitope is usually
exposed and available to
Merced,
This makes perfect sense. Many people (young and old) are NOT internet
savvy and indeed will NOT go searching for the
correct way to ‘unsubscribe’.
Adding a hyperlink to unsubscribe would solve this problem for most people.
I'm certain that there are some people
that would not see
Now for something totally non-histology related – For those of us in the
basement, who hardly ever see the sun:
Literally a once-in-a-lifetime (about every 112 years) event – Venus will pass
in front of our Sun tonight Tues June 5. Starting about 5:45 pm Eastern time,
for about 3 hours. All of
DIRECTIONS TO UNSUBSCRIBE - it's a do-it-yourself:
- from the computer you are receiving the Histonet emails:
- open any email from Histonet
- scroll to the bottom of the email
- click on the http link
- scroll to the bottom of the new link, look for a statement about To
unsubscribe from
I'd like to wade into this discuss with a couple of comments:
LABS WANTING ONLY HIGH SCHOOL GRADUATES AND/OR NON-CERTIFIED HISTOTECHS:
Yes, I'm still hearing about places like this. When I talk with the
supervisors, it's because the lab wants the person doing the histotech
job, but they only
A Google search of prepared histology slides found a company called
Carolina
http://www.carolina.com/
Click Life Sciences
Click Microscopic slides
Don't know if they have exactly what you want, but it's a start.
Don't know anything about the company. Maybe someone on Histonet does.
Peggy A.
There is the document from CLSI on Microwave Device Used in Histology
Laboratory, GP28-A. If your lab is CAP accredited, your organization might
already be a member of CLSI (Clinical and Laboratory Standards Institute)
www.clsi.org so you might already have the ability to download this.
It's
Anyone have an adenovirus control they are willing to spare? Or trade for
something else you need?
Contact me at work, please pw...@beaumont.edu
Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073
___
Histonet mailing list
Is it ONE particular case that is giving you problems, or ALL cases of
amyloid? Maybe just the control?
If the amyloid is a large deposit, that has been in the patient for a long
time, the beta pleats can get warped, and the Congo red will be very pale to
no staining. In those cases, we:
-
I think it's more to have consistency, rather than, say, a physical reason.
My opinion.
Example:
- Tech A put the shiny side down on the flotation bath, and picked up the
sections on the slide, and did an HE.
- Later in the day, the pathologist needs additional levels or some special
stains
What is it exactly that they don't like about the biopsies? What type of
microtomy errors? Too thick? Chatter? Thick-thin ribbons?
Also, are the rest of non-biopsy tissues looking OK? And, are all the
tissues being run on the same tissue processor time schedule?
We can guess at the problem,
with the Halon, you
should have a good way to flush the Halon from the room before returning to
it.
Lee Peggy Wenk
Actually I'm Lee, not Peggy.
-Original Message-
From: Blazek, Linda
Sent: Friday, February 24, 2012 4:01 PM
To: 'Elizabeth Chlipala' ; histonet@lists.utsouthwestern.edu
Subject
Several questions and comments, in no particular order:
1. What percent of bleach?
- 10% is all that is needed for biohazards. If you are concerned about the
smell, it might be too high a percent.
2. How good is your ventilation? How long do you continue to smell the
chlorine?
- If you
I'll take a stab at it.
All the subbed slides (short for submerged in a solution) have a coating
that makes them for positively charged. The lab can make a solution to
submerge the slides, or the vendor can submerge the slides and sell the
slides pre-made. Or, the material can be placed in
Acetaldehyde can be used instead of paraldehyde, to make aldehyde fuchsin.
Substitute 1.5 - 2.0 mL acetaldehyde for every 1.0 mL of paraldehyde.
Acetaldehyde is usually not a restricted drug, a whole lot cheaper than
acetaldehyde, and once opened, remains good for much longer than
paraldehyde,
The StainsFile page has some techniques, using polarizing microscopes.
http://stainsfile.info/StainsFile/stain/pigment/asbestos.htm
Asbestos are usually very small fibers, and will not show up in every
section. Cutting thicker sections sometimes helps.
Peggy A. Wenk, HTL(ASCP)SLS
Beaumont
Any chance it should have been 0.5 g lithium carbonate in 500 mL water?
Maybe someone typed the label wrong? That's happened to us before. Is it
supposed to be a 1.0% solution, or a 0.1% solution?
Only reason I'm asking is that the only place we use lithium carbonate is in
the LFB stain for
With the hematoxylin shortage of a couple of years ago (real, not imagined
in about 2007-2008), several companies tried to come up with a synthetic dye
substitute.
A little background: Celestine blue (CI 51050, also known as Mordant blue
14) is a substitute touted many years ago (late
Is there a slaughter house nearby? Call them, and have some documentation
that you are from a university - such as a memo on a letterhead.
Is there animal research at your university? Can they spare a rat?
Try to do this right before class, so there is less autolysis. Put tissue in
a plastic
Acceptable experience, for the on-the-job route (OJT), as defined by ASCP
to sit for the HT or the HTL exam is the following:
. . . you must have experience, within the last ten years, in the
following areas:
•Fixation
•Microtomy
•Processing
•Staining
So, the PA met the requirement:
- they
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