A Sakura film cover slipper will not work without xylene.Now that you have
decided to go "xylene free" just go "all the way in" and after staining the
slides DRY them in an oven at 60ºC during 5 minutes and cover.If you want
further information on "cover slipping without xylene" send me your e-
Consider the cytology lab as if it were another section of histology, like
"special stains".Staff it with at least 1 samples processor + 1
cytotechnologist.Samples should be accessioned separately.René
On Monday, August 9, 2021, 06:03:03 PM GMT-4, Richardson, Pam K via
Histonet wrote:
Unfortunately your pathologist is wrong. You will need the "rhodamine" special
IHC method to identify copper in tissue sections.René
On Tuesday, September 10, 2019, 11:08:09 AM EDT, Jennifer Phinney via
Histonet wrote:
Hello all,
I am in a veterinary histology lab. One of my Pathologis
Under separate cover I am sending you 3 papers of mine that answer your
question.René
On Wednesday, August 28, 2019, 08:45:31 AM EDT, Ingles Claire via Histonet
wrote:
Chris:
Propar from Anatech works great for us. I believe it is still advisable to use
xylene in the cleaning cycle on
Better try another counterstaining. Will be easier in the long run.René
On Monday, August 5, 2019, 01:08:38 PM EDT, Alonso Martínez Canabal via
Histonet wrote:
Good Afternoon.
It is great to be in this list again. My first e-mail is to ask
your advise about a little problem th
Either potassium or sodium hydroxide will work at 10% aq. solution. Check it
daily until soft enough.As to your "swallow" question, I "Goggled" your
question and the answer is the following"
In the end, it's concluded that the airspeed velocity of a (European) unladen
swallow is about 24 miles p
Stainers and coverslippers:Let me interject in the discussion:1- Sakura is
"hands down" my preferred option because of reliability and results
consistency2- Having to have a water free last ethanol is something of the past
(besides just proper protocol) on the other hand for years I have dewaxed
Time alone is not the only cause for antigen decay for it is also associated
with storage conditions and, as such, you cannot "predict" % decay based on
time alone.Just try one block and let the pathologist decide based on the
usefulness of what he sees in that try test.René
On Thursday, F
If you add anything, and I mean ANITHING, to your message, the message will not
be posted.I recommend you to post your problem again without the
screenshot.René
On Friday, November 16, 2018 11:27 AM, "Campbell, Tasha M. via Histonet"
wrote:
Thanks. So I got a copy of this email I sen
Terri:
All you wrote is absolutely true BUT has nothing to do with the "negotiating"
aspect Charlie us asking about.He asked about the price he wants to pay and
that will depend on his purchase volume that could determine, if his' is large
enough, may entice the seller to reduce his profit marg
Ginny:It seems you are asking for a "problem" average and that will vary per
laboratory and how the whole process is carried out.Section washed out from the
slide usually is consequence of poor processing or sectioning and has nothing
to do with IHC, although, perhaps, a wrongly performed HIER c
If "New Bouin" has no pricric acid it is NOT Bouin. You can call it whatever
you want but it is not Bouin nor it can have the "quality" provided by picric
acid without picric acid.What you wrote is simply a deceiving"sales pitch".René
On Wednesday, October 24, 2018 6:10 PM, Linda Miller vi
Charles:There is the "easy way" and the "hard way" and the differences are
minuscule:1- add what cost you ALL the ingredients for each aspect you want to
determine2- add the salary of the people doing the task related to productivity
(blocks or slides or whatever per hour)3- add-up 1 + 24- divid
Gudrun:
I have used/recommended for 10 years now graded isopropyl (2-propanol) alcohol
to dehydrate and before paraffin ONLY and when I went to Australia in 2011
found out that they had been using the same protocol for years.
You just grade 2-propanol in the same way you grade ethanol (60%→80%→90
Paula:"Optimizing" H&E to detect H. piloris is a rout you should not attempt
because H&E cannot be "optimized" for that.Giemsa is one way or, even better,
modified Steiner stain. The only disadvantage modified Steiner has is using
radioactive uranium nitrate, but I developed a procedure where 0.
Dear Dr. Rosen:
I see nothing wrong in your protocol but your photo 0950 = 09501 seems as if
there are 2 superimposed sections, one with a lot of brown particles? Is this
artifact you are referring to? Seems "too organized" to be an artifact but it
is brown and not red.
These "non DAB" reagents
Both formalin and xylene (and any other dangerous fumes) have to be avoided
during pregnancy BUT if you have an efficient fumes hood to do grossing, then
you should monitor exposure. Somebody NOT pregnant should gross with a personal
formalin badge and, depending on the exposure result, then you
Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 972-1596
> (860) 545-2204 Fax
>
> -Original Message-
> From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> Sent:
Get a DAKO IHC stainer (even if it is "refurbished")René
On Thursday, August 30, 2018 9:56 AM, Sandra Cheasty via Histonet
wrote:
Hello all,
We are in the market for a new IHC stainer. (Our Lab Vision 720
is no longer reliable.) We average about 20 slides a day, but som
Everything has been pointed out is correct BUT also pivot on "how the unstained
slides are kept".Kept in a box their "useful life" is quite short (not beyond 1
week at the most).Kept at -80ºC I have used them after years of being stored
the principle being of deep-freezing and this is "standard
The processor screen temperature reflects the actual reading of an embedded
electronic temperature probe and, as such, will be more accurate (always at the
same place, in the same way, with constant precision) something rarely obtained
manually. Therefore, use the temperature reading from the sc
Check fixation time and specially IF the Bx was left to dry before
fixation.René
On Thursday, July 12, 2018 12:38:28 PM, Charles Riley via
Histonet wrote:
My pathologists says that 1 out of 100 (his estimate) of our FNA's
cellblocks apears to be "cooked" when looking a
We incinerated itRené
On Friday, June 29, 2018 03:49:50 PM, Paula via Histonet
wrote:
Hello all.this is an on-going topic with our lab.
If anyone can comment about your experience with paraffin waste, I
appreciate that in advance.
Does your lab or state require
Not all objectives "are created equally"."In the beginning" (as in Genesis) the
objective "was created" to be used on microscopes with essentially two
different focal distances, i.e. 160mm and 170mm. By the 1950's the ∞ corrected
objective appeared to simplify somewhat the situation BUT every ma
I believe that is an extreme and unnecessary step. Formalin itself is an
adequate sterilizer, the only problem would be with "prions" and
Creutzfeldy-Jakob disease cases are not only extremely rare, but always known
or suspected to histotechs. René.
On Monday, April 23, 2018 11:
You will need a polarizing filter. The size is just the necessary to cover your
light source. René
On Friday, March 23, 2018 10:27 AM, Frances Pearsall via Histonet
wrote:
Can anyone tell me what kind (or size) glass filter to use to perform
Puchtlers Congo Red? And are the filters r
Tyrone:Under separate cover I am sending a procedure I published about this
method.René
On Friday, February 2, 2018 4:44 PM, Tyrone Genade via Histonet
wrote:
Hello,
Can the Wright-Giemsa stain be used on fixed, paraffin embedded sections?
Does anyone have a protocol?
I want to exami
You are right but, your point is?Regardless, the pathologist is the responsible
for his/her diagnosis/interpretation and the liability is his/hers.Remember
that for us histotechs, our "client" is the pathologist (always remembering the
patient behind the whole process) and our essential duty is
It is impossible to say how deep to cut into a block. First the PA must be sure
s/he was sure the tumor/lesion was in the piece submitted to process. If that
is the case, the one who casted the block must be sure the lesion is as near
the block surface as possible in a way that when the block is
I consider taking out → freezing → melting → casting blocks is worse than
leaving the tissues in molten paraffin.It seems that your Monday tissue
processing ends on Saturday. I suggest to process your tissues with a "delay"
(most tissue processers have this feature) and leave them more time in
EDTA can be used to decalcify delicate bones, such as bona marrow core
biopsies.KOH is used to soften keratin (such as in toe nails) but is totally
ineffective for decalcification.René
On Monday, May 8, 2017 2:32 PM, Dorothy Hu via Histonet
wrote:
I know someone used KOH with EDTA tog
Are the smears collected, fixed and treated as "usual" along ALL the steps?René
On Wednesday, April 19, 2017 12:54 PM, Charles Riley via Histonet
wrote:
I have been put in charge of figuring out why our pap stains are light on
the hematoxylin. Everything was filtered and used fresh as p
In 100% EthOL the tissues are completely "salvaged" and you can prepare the
program to continue the steps until melted paraffin.If there are delicate
tissue perhaps they will be "over-dried" but that is easily "compensated"
during microtomy.René
On Wednesday, April 19, 2017 9:01 AM, Lauren
At my hospital we accepted work orders from our pathologists only. If a
physician resident wanted some test, it has to be approved by our
pathologist.René
On Thursday, April 13, 2017 12:18 PM, "Horn, Hazel V via Histonet"
wrote:
I was not clear enough in my question. I'm not speakin
Do you have a bottle of "Eggnog"? Egg-albumin will have similar shelf life if
refrigerated.René
On Wednesday, April 12, 2017 7:40 AM, Nancy Schmitt via Histonet
wrote:
Good Morning-
Thoughts on shelf life of egg albumin?
Thank you
Nancy Schmitt MLT, HT(ASCP)
NOTICE: This email may
Staining is a more complex step than coverslipping. Also a coverslip on a
section has more "latitude" and can, if necessary, be removed and coverslip in
a different way. A stained section, on the other hand, is almost impossible to
"correct". Between an autostainer and a coverslipper, I would ch
What you describe as a possible scenario is absolutely possible.If your PT does
not "want to hear" about it, suggest she gets a "hearing aid" or to study
something about histotechnology or even better yet, pay attention to what a
professional on the subject (you) has to say about it. You would n
B/R recyclersRené
On Thursday, March 23, 2017 11:15 AM, Lauren Sweeney via Histonet
wrote:
Hi everyone,
We are looking into getting a new solvent recycler to recycle our xylene and
alcohol, any recommendations?
Thanks!
___
Histonet maili
Perhaps your only solution is to increase HIARRené
On Thursday, March 23, 2017 9:39 AM, Mariela Chertoff via Histonet
wrote:
Dear all,
I have mouse brain tissue perfused with PFA 4% and post fixed in formol 10%
for periods between 2 month and 1 year. I cut them on vibratome (30um) and
You should treat it as a new request.René
On Wednesday, March 22, 2017 11:25 AM, "Haines, Beth via Histonet"
wrote:
Hello all,
After some discussion on IHC billing, I have been asked to verify accepted
billing practice for the following situation:
An IHC request for 2 single antibody s
"Open" your options and try Sakura.René
On Tuesday, March 21, 2017 3:54 PM, "Flynn, Evelyn via Histonet"
wrote:
Hello all,
Our laboratory is purchasing a new paraffin embedding station. We are
considering a Leica Arcadia or a Thermo
HistoStar model.
Has anyone had good or bad
You have a special project → special tasks so your approach has to be equally
special.Large brain sections are usually stained while floating but for IH with
different and successive steps requiring very expensive reagents, floating
sections is not well suited.You should affix the sections to la
M best advise is contacting Dako and obtain from them the IHC manual, which
covers essentially everything you need to know on this subject.René
On Saturday, March 4, 2017 2:51 PM, Lynette Pavelich via Histonet
wrote:
Hello,
I will soon be starting the process of validating many cytopla
You can find all my xylene-free processing schedules at the HistoNet archives.
They use 2-propanol → mineral oil → paraffin.René
On Saturday, February 11, 2017 9:44 AM, ian bernard via Histonet
wrote:
We use Safe Clear 11 (a Xylene sub) as the clearing agent on our routine
processing
You do not have to "babysit" the procedure for it has well defined/timed steps.
You just need a timer and check the slides when required. As to procedural "dos
& donts" try to get a copy of the DAKO IHC manual.René
On Wednesday, February 8, 2017 7:34 PM, Jennifer via Histonet
wrote:
H
Never mind what other people do, just ask your legal department what to do
because this may involve legal consequences.René
On Wednesday, January 18, 2017 10:47 AM, Vanessa Keeton via Histonet
wrote:
Good Morning All!
I was wondering what everyone's policies were on releasing of pati
We used to keep our blocks on-site during 9 years. During January of each year
we disposed off 1 year worth of blocks (the oldest) and only kept those deemed
by the pathologists as interesting for our residents' training
program.Regarding slides, in 2001 we had 56 years of slides on-site and it
Improper staining at the center and falling sections are typical consequences
of poor fixation/infiltration.If you have changed nothing proceduraly, what
about somebody "new" grossing and preparing thicker tissue slices?René
On Wednesday, December 28, 2016 8:34 AM, Charles Riley via Histone
I do not know of anything published other than CAP "requirements"
(unsubstantiated)René
On Wednesday, November 23, 2016 9:50 AM, "Richardson, Pam K via Histonet"
wrote:
Does anyone know if there is a published acceptable range for storage of
tissue and paraffin blocks?
Cordially,
Pa
Once you finish the IHC procedure, the DAB reaction is very stable and you can
use Weigert's or any other iron hematoxylin.René
On Tuesday, November 22, 2016 5:29 PM, Esther C Peters via Histonet
wrote:
Could someone advise me on whether Weigert's iron hematoxylin can be used as
the c
Tyrone:A.A.Maximow's Azur-eosin, etc staining produces wonderful results but
this, and many very old procedures, essentially rest on the use of mercury
salts which produce special chemical compounds with tissue components.Any, and
I mean any, deviation from the original procedure will not produc
I sold all my old steel blades on eBay.René
On Wednesday, October 26, 2016 11:13 AM, Michelle Aono via Histonet
wrote:
Can anyone suggest a good company that works on old microtomes? Ours is not
broken, it's just in need of some TLC. Also, what is a reasonable price for
such work (w
I think your best option is to manually re-coverslip to the slide.René
On Sunday, October 16, 2016 10:00 AM, Pamela Marcum via Histonet
wrote:
Good Morning,
Although the laboratory stopped using tape years ago we are still facing issues
with "OLD" tape slide being requested for rev
Hi colleagues:I have just received the sad news that the prestigious Russian
histopathologist Prof. Alexander Matsionis passed away (see included
message).Although his name is almost unknown in our field he always was
extremely enthusiast about new histopathology procedures and helped introducin
If you already saw the drawings for the designated area, is it not too late now
to make changes?If you think you can give input, my only suggestion is that you
set your working areas in a way that they follow the workflow and ideally
should be close to the surgery suits.René
On Thursday, O
Julio:Unfortunately NBF is the OVERALL best fixative there is. ANY substitute
will be good for some things and not that good for others. Under those
circumstances what to do? Simply use LESS amounts of formalin, do it safely
keeping to a minimum its exposure.Under separate cover I am sending you
Egg white (in Mueller's albumin) will always produce a "shadowy" staining with
IH procedures y a dark background with IHC procedures.I suggest you use pork
gelatin dissolves in the water of the water bath, use (+) charged slides and
increase the drying time in the oven.Also sectioning those larg
Do you have any contacts at any old histology lab, i.e., one that has been in
operation for more than 50 years? You may find there iodine crystals and ask
for a few grams. I used to have a 500 g bottle at my lab (which began in
1947).René
On Thursday, September 1, 2016 10:39 AM, Angela Lam
Once you start substituting things in an original recipe, the outcome cannot be
expected to be what the original recipe was supposed to deliver.Iodine crystals
cannot be substituted by Lugol because, besides the iodine also contains its
salts. and alcohol. They are two completely different thing
Yes, I received it.Most probably it is a disguised "junk/spam"
advertisement.Just in case do NOT open it.René
On Wednesday, August 24, 2016 10:58 AM, "Macke, Gail via Histonet"
wrote:
Histonet,
Received this today.
What is this?
Can you look into this?
Has anyone else received this?
To
1- Make a list of ALL the tasks you delegate on this "Lead Histo"2- Quantify
each tasks, i.t. give a "numeric weight" to each of a maximum 100 points.3-
Keep track of how the "Lead Histo" performs in each and DISCUSS your evaluation
with the "Histo Lead" quarterly. This will allow the "Lead His
Jorge:The first thing is to be absolutely sure the data is worth publishing and
that the results have scientific relevance.If this is the case and both you and
the other contributor agree I think the data should be published.In no way you
should eliminate the data obtained by the other individua
Apply gently heated water on the sections in a way that the gelatin is washed
out.René
On Tuesday, August 16, 2016 4:48 AM, Monica Aguilera via Histonet
wrote:
Dears,
I was wondering if some of you might have experience in the following:
We have had a lot of problems cutting WAT tiss
I used to have several compartmented plastic alphabetized boxes with enough
empty spaces to accommodate "new arrivals". If the spaces were used-up I just
added a new box. Since they occupied several shelves, each shelf had the
lettering identifying the boxes in each one.René
On Tuesday, Au
Usually you do not pour 10%NBF onto a specimen; you place the specimen onto a
container/vial with the 10%NBF.René
On Tuesday, August 16, 2016 9:29 AM, Mike Pence via Histonet
wrote:
I know this might sound a bit crazy, but does anyone have a written policy and
procedure for dispensing
Get regular metal cabinets used to store garage items.They are sold at any
general store (such as HomeDepot or Walmart).René
On Thursday, August 11, 2016 12:05 PM, Atoska Gentry via Histonet
wrote:
Hello, I work for a research facility and we currently have an archive of
several forma
anufacturers, NOVOCASTRA laboratories BUT I
have never heard that it has bought Sakura instruments. It would be nice if
somebody has reliable information about this alleged acquisition.René
On Monday, August 1, 2016 4:02 PM, Rene J Buesa via Histonet
wrote:
I don't know now, but some year
I don't know now, but some years ago Thermo instruments were less that
reliable. Try Leica or even better Sakura.René
On Monday, August 1, 2016 3:20 PM, Mary Faith Encarnacion via Histonet
wrote:
Hey HistoNet,
Thanks to everyone who helped me out by providing their opinions on
embeddi
Request a DAKO demo.René
On Thursday, July 28, 2016 8:01 PM, Gmail via Histonet
wrote:
Hi all,
We are looking into getting a new staining platform for our IHC lab. I would
appreciate any feedback from your experience regarding ease of use, how long
maintenance;daily , monthly takes t
Use a regular one blade pocket knife (as I used to do).René
On Thursday, July 21, 2016 2:49 PM, Lauren Sweeney via Histonet
wrote:
Hi all,
My lab is in need of some tools to scrap the paraffin off the edges of the
blocks after embedding. Does anyone have any recommendations for me?
Gomori's René
On Thursday, July 21, 2016 12:04 PM, Anita Buchiane via Histonet
wrote:
Does anyone out there still do the retic by hand? If so, can you share which
procedure you use? Thanks
___
The information contained
Place your molds in a 2% dishwasher soap boiling solution for 5 minutes → was
in running water for 5 minutes → dry in a convection oven at 60ºC for 10
minutes and your molds will be ready to use.As a "release" solution use a
mixture 1:1 of 2-propanol and mineral oil (light weight).René
On
I always used Auramine at room temperature to identify TB bacilli in tissue
sections with fluorescence filter, never used heat and the results were as
expected. Bancroft is the one describing the procedure using heat for
auramine/rhodamine procedure but auramine alone at room temperature is
eno
Your tech has an "above deserved" expectation.How would you even consider
promoting somebody who is not qualified to even be HT certified to Lead
HT/Coordinator?This is disrespectful for those who reversing that position have
been unable to achieve it.It speaks volumes about your tech aspiration
Angela:"Pale" results are the trade-off for great quality very thin "2 µm"
sections but you can always improve intensity somewhat .1- your "regressive"
stain, if it is "modern Harris" has the inherent problem of lacking mercury
chloride and it is little you can do about. Perhaps if you use "pro
What you need to do is to communicate to everybody where the kits are, and
place them where it is more convenient for you. Once everybody knows the
location, a good sign is always a plus.René
On Tuesday, June 14, 2016 10:15 AM, Anne Murvosh via Histonet
wrote:
Do spill kits need to be
It seems to me you are processing too much unless the slices are 3mm thick or
more.I suggest you to cut the dehydration to 45 minutes (the sequence seems
OK)Reduce the pure 2-propanol to just 2 changes (30 min is OK)Add 1 change of a
mixture 1:1 of 2-propanol and xylene + 2 xylene stepsthen to p
If your 1.3% picric solution is in distilled water, there is not much you can
do about it.If it is in acetone 38.4 mL contains 0.5 g of picric acid to which
you can add 361.6 mL of acetone to get your desired concentration.René
On Monday, June 13, 2016 3:24 PM, Jennifer MacDonald via Histon
It seems you have "baked" and "unbaked" slides.4ºC storage is always more
expensive and "baked" slides are keep very well at RT.I think your first step
is to ask around who would like those specific slides.If they will be used in
the future, "bake" those "unbaked" and store all at RTRené
O
Would you share what you receive for the amusement/benefit of us all?René
On Tuesday, May 31, 2016 9:15 AM, Charles Riley via Histonet
wrote:
I am trying to do a histology tip of the week for my new histo team as a
way to help them learn some new ways to do troubleshooting. I've run out
Hi Mike:The steps you desrcibe are wrong.After you finish staining your PAP
smear, just wash them in the last ethanol → oven dry at 60ºC for 5 minutes or
as required if the smear is too thick and when completely dried →
coverslip.This final drying has to take place at temperatures above room tem
Photomicrography could be affected at high resolution (immersion oil
objectives) but probably could be eliminated if the microscope table is
isolated from the floor with some vibration damping device.René
On Tuesday, May 17, 2016 9:52 AM, Terri Braud via Histonet
wrote:
With experienc
You are right. Bleaching is a "rough" procedure for the "survival" of sections
and if on top of that you left the section overnight in DiH2O that is a recipe
for disaster, as the one you experienced. Try to do the whole procedure during
the same day.Additionally it seems to me that 6h in potassi
I would be concerned with potential cross-contamination. In my lab we had 2
staining instruments, one for cytology and other for histology.René
On Tuesday, May 10, 2016 10:59 AM, "Mullen, Mary via Histonet"
wrote:
Hello all,
I work in a small, low volume community hospital and was r
Picric acid is an expensive reagent useful in many histology procedures.The
advise you received of adding water is a good one.Humid picric acid will not
explode at all. Why waste a good reagent?Keep humid, you will eventually used
it.René
On Thursday, May 5, 2016 3:24 PM, Mca Werdler via H
As I see it, there are 3 main objections about using human saliva as an amylase
source.In order of importance they are:1- you will never know the actual
concentration of the amylase and this will produce reproducibility problems.2-
along with the saliva you will introduce bacteria that may end b
Productivity and quality sake, Sakura film coverslipper has no match. If you
use Sakura tape and the xylene dispenser is properly calibrated, storage is not
an issue.Sakura stainer was also what I used at my lab and I highly recommend
both.René
On Wednesday, May 4, 2016 3:19 PM, Jenn via H
I tested those you mention and leased/used the one from DAKO and "never looked
back".René
On Wednesday, May 4, 2016 12:13 PM, "Murphy, Valerie via Histonet"
wrote:
Hello Histonetters,
Our tissue core is interested in purchasing an IHC instrument. It would be
used for the more routine
My impression is that your problem is during the decalcification step. It
cannot be hurried and has to be in EDTA at pH 7All reagents have to be prepared
in pH7 phosphate buffer.The inconsistency resides in the fact that not all core
Bx are the same regarding thickness, tissue condition or size.
As I see it, the best solution is "1"Even more: if the piece of tissue is large
enough,→cut 1 section and stain → select at least 2 (+) areas→ divide the block
into 2 blocks each containing one of those 2 areas and by doing so you would
have duplicated the number of possible (+) sections.René
Thank you VERY MUCH!René
On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet
wrote:
To My Lab Colleagues:
As my intent has never been to sow discontent or rancor, I think it is for the
best if I no longer post links to my blog, lab related or otherw
Bryan:1- who gave permission to Dr. Raff?2- how we know the permission was
given?3- what percentage of HistoNet member gave the permission?4- why Dr. Raff
is so stubborn to keep posting what ever he chooses in spite of the rejection
of probably more members than those who "gave him permission"?5
This is not a good practice and can lead to inconsistent results.Always use
freshly prepared DAB sol. to finally be able to see the end product of the
costly and important IHC procedure, it is worth it.René
On Monday, April 25, 2016 6:27 PM, Andrea Calhoun via Histonet
wrote:
Hi Liste
Timm's silver stain always!René
On Wednesday, April 20, 2016 12:51 PM, Gudrun Lang via Histonet
wrote:
Hi all!
Which stain would you prefer to demonstrate copper? Rhodanin or Victoria blue?
Thanks in advance
Gudrun
___
Histonet mailing
Amen!!!But you have to concede that Lester is a very persistent, almost
obstinate individual, probably used to impose his will and this postings are
just an example of it: he likes his blog and tries to impose it to everyone.
Evidently he has all the time in the world and just does not know wha
Before deciding, ask for a "demo" from Sakura.René
On Monday, April 11, 2016 11:02 AM, Lauren Marie Hegner via Histonet
wrote:
Hello all,
Our lab is looking for a new automated H&E stainer and I was wondering if
anyone out there has had any experience using Ventana's Symphony System,
Sudan Black reacts only with protein-combined fats.René
On Saturday, March 26, 2016 11:20 AM, Joanna wrote:
How about Sudan Black stain?
> On Mar 26, 2016, at 4:32 AM, Rene J Buesa via Histonet
> wrote:
>
> The only problem I see is that the fat will be preserved, as y
The only problem I see is that the fat will be preserved, as you wrote, as a
black osmium oxidate but you will not be able to use any "standard" fat stain;
otherwise it will work.René
On Friday, March 25, 2016 2:41 PM, "Hobbs, Carl via Histonet"
wrote:
Fix the tissue in Formalin, wa
To embed the tissues with paraffin you HAVE TO dehydrate the tissue. This is
usually done with either ethanol of 2-propanol but essentially all dehydrants
will remove fat so you are right, the way to go is going frozen sections.René
On Thursday, March 24, 2016 2:24 PM, "Dessasau III, Evan v
How did you manage to deal with the about 0.5 m of blubber?Was it the skin of a
new born whale? I just to not understand, but you have to completely eliminate
all the fat and increase your processing protocol (infiltration specially) to
have some chance of getting any relatively "decent" section
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