On Date: Mon, 23 Jul 2018 18:31:48 -0400
From: Mary Ann
Subject: [Histonet] Positive PAP
Mary Ann wrote "Help! My pathologist has asked that a positive patient be run
down with our PAP stain for QC.
Point me to a reference to counter this request."
Hi Mary Ann - First of all, my sympathies. Thi
We are using a Leica 2235 and love it. Manual or Auto mode, the control panel
is on a moveable pad, so can be used anywhere. It has a foot pedal if you like
that too! The techs took to it like a duck to water and it cuts like butter!
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Labo
Are you referring to a Pap stain control? If so, we run a self made buccal
smear every day to check for stain quality.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comf
Just an idea. Why not use SEM? It seems like it would be so much easier.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal
2. MMA or Epoxy Embedding (J
For all stains, we report the control verification on the patient report when
reporting the stain results, and we run negative tissue controls for our
organism stains, AFB and GMS
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadow
We use:
1. Report TAT
2. Discrepant pathology reports
a. Internal vs External report correlation
b. Frozen section vs Final diagnosis correlation
c. Amended reports
I liked the idea of piece count discrepancy or even poorly processed blocks. I
nev
When will this company ever learn? I'm at my wit's end with failed dispensers
from Roche Ventana. The volume of time I've wasted on this issue with this
company is monumental. They refuse to give true tracking information on the
dispensers. We have a relatively new Ultra. The instrument is l
Hi Nancy - Please see my answers filled in below. I hope that helps.
Sincerely, Terri
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal
Message: 1
Date:
We use a backup generator for power outages, and also, all critical instruments
are plugged into an appropriate sized UPS (Uninterrupted Power Supply) to hold
them for about 2 hours of uninterrupted power until the generators kick in.
Basically what Allan Wang said in his reply.
Just remember th
Have you tried Polyphenyl Yellow 8G instead of Tartrazine? Just a suggestion
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal
Today's Topics:
1. Attwo
Just another note: You can order unstained tissue microarrays with the
prerequisite number of cases, both positive and negative, and stain your
validation all on one slide. I've done this for years and for 3 different
validations of entire IHC platform changes, ranging from 40 to over 100
ant
Hi Allison - We couldn't afford the program offered by our LIS vendor, but
since they are published annually, we have a fabulous transcriptionist that
updates and reformats all of ours every year. She saves them as a "quick text"
in the LIS. They are accessible to all who are dictating/transcr
I'm certified in EM. My suggestion would be to use SEM as opposed to TEM. The
scanning electron microscope (SEM) can also be useful to reveal morphological
features of isolated organisms as well as for diagnosis. There are numerous
articles citing this technique. I believe the UC Berkley has an
After 40 years of using primarily Miles/Tissue Tek, we recently purchased 2
Leica ASP 6025. With the exception of a small glitch with faulty sensors, and
a learning curve to get used to them, I would highly recommend them. We had
replaced an ancient K series(with the magnet) and a VIP 5.
The q
I wholehearted agree with Linda. Constructive criticism is one thing. Picking
apart the book, such as the title, is petty (and ignores the fact that "Hack"
is also defined as: a strategy or technique for managing one's time or
activities more efficiently; also to "cope"). Also, the criticism
In response to the request to post the new phase II CAP requirement on cross
contamination :
**NEW** 08/21/2017
ANP.11680 Cross Contamination Phase II
There is a written procedure to prevent cross-contamination of specimens during
grossing.
NOTE: At a minimum, cleaning (e.g. wip
Hi Roy! The "Germinator 500" Sounds like something from Phineas and Ferb.
Hahaha.
Anyway - The mandate from CAP is designed to clean the instruments, not
sterilize them. If the instruments aren't cleaned before stuffing them into a
250'C trough of glass beads (the Germinator), you will just
I just wanted to share a quick review of the new book "Histology Hacks", by
Michael Backhus. It is the kind of book that brings together all of the little
tidbits that a tech picks up along a lifetime of working and networking with
others. It is well organized and the information is presented
Please be sure to clear any use of a "cutting test" during the interview
process with your institution's legal department. If an interviewee should cut
themselves, the liability could be steep. If you have a clearly defined
minimum cutting speed/quality, a better solution would be to hire the
We cut levels x2 with no unstained. We've never had a problem going back to a
block for additional recuts or specials. We date all our precut IHC slides
(patient or control) with an expiration date of 6 weeks. Slides cut for
histochemistry stains seem to have no expiration. Hope this helps.
We contract with an outside company to come in and perform a sound monitor once
a year in each room of the lab
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and
Hey Scott - Our interpretation of CAP requirements for IHC Controls is the same
as yours. Our Her2 control is a microarray with 3+ case and a piece of normal
skin to serve as our negative tissue control.
If you have normal breast tissue adjacent to your tumor control, that can also
serve as a
Although we've used several brands with good success, our most consistent
performer has been the Sakura Tissue Tek Glas Mounting Media #6419. When
coverslipping, either automated or manual, the secret to avoiding air bubble
during storage is to insure that the correct amount of media is dispense
We give the worklist to the chief Pathologist and let him make the assignments
on a daily basis. They are usually divided up by complexity. In the absence of
the Chief Path, then I make the assignments.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
164
We love the Aldehyde Fuchsin with a fast-green counterstain. Once the initial
working stain is made up, it is a fast and easy stain to perform, not to
mention just pretty - purple fibers against a green background.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer
I don't think it sounds like a strange question at all, but the quick answer is
yes, it is perfectly legal and acceptable for an OJT Lab Assistant to be
temporarily assigned to supervise an HTL. As long as it does not break any
local/state requirements for licensure, or in the case of a unionize
Actually, the verification of instrument performance is a CAP All Common
Checklist Requirement and should already be a part of laboratory procedure.
COM.30550 Instrument/Equipment Performance Verification Phase II
The performance of all instruments and equipment is verified prior to init
Just use a microarray. They can be purchased in all sorts of varieties with
hundreds of cases on one slide. I use and recommend U S Biomax
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 21
1. Send-Outs (Cristi Rigazio)
Our TAT for send-outs is 1 working day (M-F). The office staff here use the
"Slide/block Send Out" function in CoPath to track all material moving in and
out of Anatomic Pathology and it really speed up retrieval of case material
3. Cassette printers - mul
Another reason that IHC is used instead of IF is with IHC, one preserves the
ability to see tissue/cell morphology through Light Microscopy at the same time
as the visual IHC label. Morphology is difficult to see with IF, with the
exception of the fluorescein labeled area.
Terri L. Braud, HT(
Our hospital has a policy against releasing soft tissue to anyone except a
funeral home. Realizing that some religions require one to be buried with all
their body parts, release to a funeral home meets those requirements without
someone finding human remains years down the road, with no record
Such a shame that you have to choose. But, that said, a coverslipper. Hands
down. If nothing else, due to safety and exposure reasons alone. H and E
stains can be unstained and restained, easily corrected, and easily
accomplished in batches and the racks have HANDLES. But with coverslipping
I know many, including staff here, that prefer to use "chux". They are very
cheap, come in various sizes and adsorption levels, latex-free, with a highly
absorbent core and soft, quick-drying coverstock so you don't get lint in your
specimens. Use your favorite search engine to look them up. A
We have just replaced our 2 old tissue processors and have one very old (1989)
VIP3000 floor model, the one with the magnets. It is in working condition, but
will not hold the battery charge for the backup battery. We have been using it
with a UPS, for 6 runs every week. If anyone is intereste
Coming from an ancient histology tech who did IHC back in the dark ages, before
automation, hand staining is a piece of cake.
A couple of hints:
Use a "pap" pen to circle your tissue on the slide to form a barrier to keep
your reagents puddled in a concentrated spot and to minimize reagent use.
U
Our policy is to not release any wet tissue except by subpoena, except for
purposes of burial, and then only released to a funeral director.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 21
My response:
My first question is what temperature are the blocks that are too brittle? And
at what temperature are you trying to cut? Blocks stored in cryofreezers at
-70o C or less are far too cold to cut without brittleness. My suggestion
would be to pull the blocks and put them into the cr
For those of you working in smaller hospital Histology labs (not staffed 24/7)
- How does your OR/Lab handle getting specimens into fixative?
We currently use small/med prefilled containers for smaller specimens. Does
the OR dispense formalin into larger containers? Do you refrigerate unfixed
To clarify, the mint date on the Queen Victoria 0.25 ounce gold piece is the
"Manufacture Date" not the expiration date. Also, since it clearly does not
have an expiration date, regulations state that an expiration date must be
assigned to the coin. Then, the effectiveness of the gold piece in c
Just a word to the wise: Although I certainly appreciate the well-referenced,
sound science behind Tony Henwood's advice, as he also pointed out, if your lab
is CAP or CLIA inspected, the regulations are quite specific that expired
reagents cannot be used for patient work. I agree that it is a
My first 2 items to check whenever I have uneven IHC staining are (1)
Inadequate deparaffinization, (2) bad lot of charged slides - yes this can
cause terribly streaky or spotty staining, and since you are using the Bond,
perhaps there was an issue with coverplate placements.
Just things to cons
This should help...the IHC "test" is the pathologists' INTERPRETATION of IHC
stain. The stain procedure, validation of protocols and controls, and lot to
lot validation must be signed off by a pathologist.
Any tech that has demonstrated competency in performing the procedure, can
perform IHC s
Re: Her2 by IHC
We reflex 1+ to FISH, too
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
___
Histonet mailing list
Histonet@lists.
Hi Cristi -
We have a large breast practice and we worked with the surgeons and surgical
staff to include on the requisition:
"Collect time"
"Time in formalin"
Then at gross, the PA or pathologist grossing calculates "Cold Ischemic Time"
and "Duration of Fixation"
All breast specimens are handle
We use an awesome little band saw made by IMEB, Inc. It has a small foot
print, 4 blade types and added accessories for a super lab bone cutting
station, and best of all, very inexpensive.
It can zip through the densest of bone, or the most delicate. It can be set up
as a water cooled station
Hi fellow Histonetters-
I'm looking for any feedback from working labs using Thermo's Excelsior
Processor. If you can help, can you please also include the age and usage on
your instrument?
Thanks, Terri
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
16
We provide formalin training and small volume spill kits and neutralizing wipes
for all physician offices owned by our health care system.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-
I just instruct the service personnel to include a single statement on the
service report that the instrument has been tested and is functioning properly
following service. Worked for the last inspection.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1
Our Prisma is hooked up to tap water.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
3. Water for H&E Stainers? (P Sicurello)
4. Re: Water for H&E Stainers? (Elizabeth C
Hi Gareth! BioCare has a wonderful instrument, the Oncor, that has a 30 slide
max, with full onboard antigen retrieval. Super simple to operate. We were a
Beta site to test and all the techs really liked it.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospit
Just wondering why you are compelled to make all the reagents in house for the
Movat's stain. I have used the kit k042 from Poly Scientic with fabulous
results (and no, I have no gain from recommending them).
Their solutions are wonderfully labeled and can be replenished individually, or
by ord
The ER/PR benchmarks are those published in the notes section of the checklist
question. We use the lowest numbers of the ranges. We track , patient age,
cancer type, tumor grade, and positive or negative results, then just run the
numbers. We also add in some extras, such as cases positive fo
If you've not been made aware before now, be warned. The Department of
Veterans affairs (DVA) has proposed a rule that would authorize any Advanced
Practice Registered Nurse (APRN) to not only order and interpret lab tests, but
also to perform, supervise, and direct lab testing. The rule follo
We used to put them in a large stainless steel basin, with a little bit of
Alconox brand detergent. Fill the basin with hot water and bring to a boil on
a hotplate. Turn it off, let the water cool for a couple of hours and all the
paraffin would rise to the top and solidify. Just pick the par
Respectfully, a public forum is no place to discuss personnel issues on such a
personal level.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
4. Certification (Adesupo, A
I must be missing the obvious. Why can one no longer use Bouins? We still use
Bouins.
Terri L. Braud, HT(ASCP)
3. Testicular biopsy for infertility (Cartun, Richard)
Message: 3
Date: Tue, 28 Jun 2016 20:41:47 +
From: "Cartun, Richard"
What are people fixing testicular biopsies in to eva
Wow, you sure picked a doozy of a subject. Unless you work in a state that has
specific licensure requirements, there really is nothing to prevent a
non-certified Histology tech from doing the same duties as a certified tech
In my opinion, it's more a matter of skill sets, and as we all know, th
We used to have problems with fluids/orders, but what we put in place seems to
be working.
1. All fluids are received by central processing. If a fluid has no orders,
then central processing calls to have the orders put in. Cytology does not
accept fluids without orders. We also encourage (thou
Annual Formaldehyde safety education should be part of your on-boarding
process, as well as annual education, per OSHA
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
4. Form
Cut slides stored at room temp seem to last quite a long time for routine
stains, however, cut slides do demonstrate a loss of antigenicity for IHC
staining, depending on the antibody.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
M
We had the same issue a few years ago with the Ventana HP antibody. Then we
tried the antibody from Zymed and it was so much better. Now we use BioCare's
antibody, and it is very clean.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pik
I don't have a diagram, but there is a really good SQ CoPath list serve where
you might find your answer.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
2. Question - CoPath
Hi Jeanine -
I can't remember the last time that I had any cross contamination in the tissue
processor. If the tissue is properly grossed without contamination, secured
within a cassette (we use blue pads for tiny hard tissue; lens paper for
fragile biopsies, such as liver; and nylon mesh bags
With experience and certification in both, I think that problems from ambient
vibrations are much less severe in Histology than EM. There are special
vibration dampening worktables, often used for EM, that would eliminate the
problem for cutting stations, or any other sensitive equipment, and s
We run both stains on our Sakura Prisma, but they share no common reagents
except for the final Xylene unload station. We do use the combination CytoKwik
stain, which combines the OG6 and EA50 steps, to reduce the number of steps in
our Cytology stain procedure. We only run non-gyn, too. It is
We've used Leica for 8 years and everyone here loves it. We have it loaded
with Leica brand plus slide, Leica regular clipped corner slides, and Thin Prep
Slides from Cytologic. It was easily interfaced with CoPath, seldom jams, and
we average about 1 unexpected service call per year in additi
This is an repetitive stress injury and is not to be trifled with. I know that
I have a tech that occasionally uses a wrist brace to embed, but also to sleep
with at night.
I strongly recommend using ergonomic friendly embedding forceps available from
selected Histology supply companies. Also
BIG Congratulations! To Patrick Lewis on passing your HTL
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
7. I did it I am now a certified HTL (Lewis, Patrick)
Message: 7
Date:
We, too, had the same problem. The GI rooms were cutting telfa pads so they
would fit in the formalin containers, and the pads were falling apart. We
finally asked that they just put the specimens directly into the formalin. That
way we can decide in the gross room, the best way to submit the
We cut 3 levels, 40 microns apart, all mounted on the same slide
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
___
Histonet maili
For those of you that are CAP accredited, and use a cryostat with UV
disinfection, how do you get around the CAP requirement listed below? We were
told the UV disinfection did not replace wiping down with a tuberculocidal
disinfectant at room temp. Do you have an approved tuberculocidal disinfec
Our institution follows the CPT coding and associated billing, just as you
explained. For those surgeries done within our facilities, there is a specific
list of specimens which must be submitted. This was agreed upon by med staff
and is listed in the bylaws, in response to the following CAP qu
We are a similar sized hospital with an 8,000/yr Surgical load of mixed large
and small cases. We process 1000 Non-gyn Cytology cases, assist with FNA
collection in Interventional Radiology. We also assist with about 130 bone
marrow collections, including smears and processing. There is mysel
Our lab is seeking an Pathology Assistant for an immediate temporary position
for all gross responsibilities. The position will be approximately 10-12 weeks.
We are a medium sized hospital in the NE suburbs of Philadelphia. We have a
friendly technical and medical staff of 2.5 pathologists and
LOL...Shrinkage...heh, heh.
But seriously, there should be little to no gross shrinkage from formalin
fixation and if the specimen is properly fixed, then there should be very
little gross shrinkage as it is dehydrated. That is supposed to be the point!
If someone is getting 30% shrinkage, the
There is no CAP requirement that a person has to be certified or qualified in
any way to perform Histology or Histology Assistant duties. As long as you can
document their proven competency, they are good to perform any procedure. The
only exception in the Histology lab, is if that tech is req
We LOVE our Leica printers. IPS and IPC. We've been running them for over 8
years with few problems and little down time.
We recently interfaced them to CoPath and it went very quickly and smooth. Now
we are loving them more than ever.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
La
Hi Charles - Not to worry. Many of us Histo folks don't have a Cytologist to
help. Beth is spot on in her advice. I just wanted to add Orange G should
appear yellow to orange; 15 sec to 1 minute is the usual range of staining
times. EA is often problematic because of fundamental limitations
I've had experience with several glass coverslippers over the years. While
I've not tried the newest/latest/greatest of other brands, I've been running
the Sakura Glas coverslipper for 8 years without a hitch. It has been very
dependable, with little downtime, and extremely easy maintenance.
If a patient or an outside hospital/doctor requests a consult, we encourage
patients to pick up their slides at the same time as they pick up radiology
discs, medical records, etc. If we need to fedex slides/reports to another
institution we ask the receiving facility for their Fed-Ex number.
UAL (United Ad Label) has a label guns, similar to the old paper labeler which
puts wonderful labels, your choice of rec'vd date, date open, date exp...or any
combination of the 3.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Mead
Dear Charles -
If your samples are so soft that you have to turn up your microns in order to
section them, then the tissue is underprocessed. Many people make the mistake
of equating "underprocessed" with "under fixed". A quick way to see the level
of processing is to add 50.mls of 1% Alcohol
Just my 2cents. I've had good experiences with the Artisan, both before and
after it was purchased by Dako.
It's very user friendly and very reliable.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3
Our policy calls for handwriting slides, then applying printed labels at the
completion of the stain/coverslip.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Today's Topics:
Have you tried Sigma's ImmunoHistoMount? It was what replaced Crystal Mount
which used to be my favorite.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
**
_
Do these steps in order:
Take the total cost of your supplies + total hours salary average, for set
period (usually use 6 months), and then divide by the number of blocks for a
cost per block (ie. 0.80/block)
Do the same for slides to arrive at a cost per slide. (ie. 1.25/stained slide)
Calcu
All of our QC sheets around the department are on obnoxiously colored
fluorescent orange clipboards. I use 3M Command hooks to hang the clipboards
near or on the instrument or area. I use a single clear plastic page protector
clipped on top of the paper to protect them from splashes, etc.
Look
Dear Baffled -
Could there possible contamination from other specimens that are being
processed at the same time? For example, skins or other tissues with inked
margins?
Just thinking out loud.
Pondering, Terri
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hos
Just a note: The results of the stain are supposed to be elastic fibers:
Blue-black to black. If you stain something with a bluish tint, then
counterstain over it with Van Gieson's (red)...
Well, red and blue make purple. Even the picture that accompanies the kit in
the catalogue shows the f
Hi Nancy -
Here is what our lab does.
All preps (slides)are fixed in individual containers before being batch
stained.
We stain smears separately from FNAs, and smears and FNAs, separately by case.
All fixed thin preps are stained together.
With smears or FNAs, we insert a clean slide (labeled
Dear Amy -
With 30+ years of experience of running an extremely tight ship, I can see how
you would be drowning in work from the loads you are describing. I will email
you separately, 2 articles concerning calculation and documentation of
workload. One is from CAP in conjunction with NSH, and
, 2015 3:24 PM
To: Terri Braud; histonet@lists.utsouthwestern.edu
Cc: Cassandra P. Davis
Subject: RE: Frozen Section WL and Billing
Aren't fs CPT codes 88331 and 88332?
From: Terri Braud via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Friday, Augu
We set up our system this way -
Example:
A specimen for frozen is sent. You cut 2 blocks of frozen tissue, 2 levels ea.
The PA/Pathologist submits 3 additional blocks for a total of 5 blocks on the
specimen (1-2 are the previously frozen, 3-5 additional tissue) to be cut at
one level each.
1. A
Hi Laura -
We print all of our slides using the Leica printers. Even batch cut control
slides are pre-printed. The only time we write on the slides is if we cut
patient tissue applied to the control slide for IHC staining. Then we will add
the Block ID and patient name by hand to the control
Med size hosp lab - not here
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874
2. IHC Weekend Coverage (Cartun, Richard)
3. Re: IHC Weekend Coverage (Victoria Baker)
Message
I have an easy answer for you. Don't use freezing spray in a cryostat. EVER.
It creates dangerous airborne microdroplets that can linger and transmit
disease within the room for hours. Here are 2 case documentations, and there
are others. If you can't cut a section frozen at cryostat temperatur
The regulations vary from city to city and are managed at the municipal level.
It is usually set and managed by the city chemist or the head of the EPA
(Environmental Protection Agency) for your area. It can also vary when
compared to the total volume sewage discharged from your facility. Bel
For us, unexpected findings are noted in the report as such. The pathologist
calls and personally notifies the surgeon. The date/time and person called is
noted in the report.
Example:
NOTE: Unexpected findings of discussed with Dr on 7/31/2015,
1115.
Hope this helps.
Terri L
H&E Stainer -
I love our Prisma and Glas coverslipper. Such a workhorse. Easy maintenance.
Almost never any downtime.
LCS - Liquid coverslip is a type of oil, and should rinse easily in your
alcohols during run down. If you are seeing water, perhaps it is held in place
with your slide labe
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