[Histonet] old bones
Thanks all for suggestions ... as always you guys rock! Sadly, this may almost be my last post - after close to 30 years in the lab, I'm moving into another arena all together - environmental health! So this may well be farewell to all you great guys and gals who have provided help and advice over the years -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thanks for all the good wishes
AlsoI'll try to get the UNSUSCIBE UNSCRIBE UNSUSCRIBE thingy right when the time comes! -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) A good head and a good heart are always a formidable combination. Nelson Mandelahttp://www.brainyquote.com/quotes/quotes/n/nelsonmand101682.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] techniques for archaeological bone
Hi all... Had someone in here who is interested in sectioning old bones - any ideas as to what would be the best embedding medium? These are bones from a graveyard more than 100 years old - not fossils. Decal is out of the question - so it would have to be a resin of some sort. Tips, ideas, contacts and methods would be very welcome at this stage -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Klinipath coverslipper
Hello Histoworld! Has anyone used the Klinipath coverslipper? I am thinking of buying one and was wondering how it compares to the Sakura tape coverslippper. Thank you -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 101 steps to better histology
Hi all, Just thought I'd share this with y'all. I have just received a booklet from Leica Microssytems 101 steps to better histology, which i think is an excellent overview and ready reference for practical histology. It has great photos of common problems such as sections contaminated with squames, incomplete blueing, bubbles in sections etc. Disclaimer: This is my personal opinion. I have no interests in Leica other than purchasing their equipment now and then -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Thank you
there's always good old pencil (HB) as a backup On Thu, Apr 12, 2012 at 12:05 AM, Patsy Ruegg pru...@ihctech.net wrote: The best markers I have ever used are called KP Markers, they were off the market for a while, but they are back and we get them from Mercedes Medical, just got a new batch and they are wonderful just like the old KP markers, we won't have anything else in the lab. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Wednesday, April 11, 2012 2:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thank you Hi Everyone: I want to thank everyone who gave me advice concerning my cassette labeling problem. I am trying out three different types of markers pens: StatLab, Leica, and NewComer Supply Histo-tec brands. We will also make sure to let the ink dry before putting the cassettes into formalin. If we still have a problem, we will experiment with a different cassette. Thanks again. Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA 617-855-3592 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] GLASSWARE CALIBRATION
1ml of water =1g mass. could you use this to determine the degree of error between calibration and real volume? On Wed, Mar 28, 2012 at 10:51 AM, shehnaz khan shehnazs...@gmail.comwrote: Hi netters, Could someone please shed some light: 1. How is calibration for glassware performed on non-Class A glassware? 2. If Class A glassware is used but no certificate is located - does it still require calibration? Thanx again. S Kahn ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Goldner's Trichrome for Osteoid
As far as i know, osteoid will not show up on a decalcified section. On Thu, Mar 8, 2012 at 6:00 PM, Grantham, Andrea L - (algranth) algra...@email.arizona.edu wrote: Hopefully a hard tissue guru is out there and can help with this. A PI here is asking about doing a Goldner's stain on mouse femur. I'm not set up to cut sections of undecalcified bone so my questions are: 1. can this stain be done successfully on decalcified bone? 2. if so, what type of decal should be used - EDTA? 3. I have googled the stain but didn't see what the preferred thickness was for this stain. Need this info fairly soon! The bones are on the way. Thanks!!! Andi G. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Regarding Bone-Implant Contact Percentage
Hi how are you hoping to quantitate? do you have a software programme? Our latest paper on titanium implants (in press) measure BIC expressed as a percentage of implant surface examined. On Mon, Feb 13, 2012 at 1:57 PM, ragamouni sravanthi ragamouni.sravan...@gmail.com wrote: Hai,... Can any one help me with how to calculate percentage of bone-implant contact with implants in bone sections. Please can you anyone give me detailed explanation and the required equation.Ineed it in urgency. With Regards R.Sravanthi ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Interview Questions
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Re: [Histonet] Music in the Laboratory
I think there is nothing worse than being forced to listen to music that you don't like or enjoy. If the person works alone, then fine, they can listen to what they want within reason, but if there are others well then, one has to be considerate. Check with yr safety guys as to whether mp3 players s with headphones are allowed. my 2 c worth On Wed, Jan 18, 2012 at 4:37 AM, tracz...@aol.com wrote: Greetings. I would like to know what other histology laboratories allow for music players while working. Do you have formal policies about music content or volume? Do you allow lab space doors to remain closed to muffle the volume of what is being played? Are headsets allowed? I am a terrible judge of this because I personally prefer to work in a quiet environment. I am trying to be open minded, as long as the work gets done. However, one of the techs had a song playing today that I believe was inappropriate for general listening in the lab. Am I just out of touch? Is that dang F word just something I'm going to have to learn to accept? Do you have a written policy? When/how/why was it implemented? I should mention that it's a small private lab, with minimal patient traffic. We do see our share of FedEx, UPS, sales service reps. Your ideas on this is very much appreciated. Dorothy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] clostridium and bacterial spores in tissue sections
I used to flame tissue sections regularly when doing a ZN for TB...a wisp of cotton wool held in forceps, dipped in alcohol and lit with a match. A few quick passes under the slide and bob's yr uncle. worked fine, until the day came that I ignited some acetone fumes in the sink and fireballed my eyebrows off! Gentle heating in a incubator also works On Sat, Jan 14, 2012 at 8:34 PM, Rene J Buesa rjbu...@yahoo.com wrote: When I was in pre-medical (59 years ago, imagine!) we used the same stain you are referring to that involved Ziehl-Nielsen and light green as counter stain with heat, so much heat, that a flame has to be applied to dry the smears. In Peter Gray's book there are about 40 methods described,none for tissues. I am not sure a tissue section will survive, but, why not, just try it, and let us all know. René J. --- On Sat, 1/14/12, Elizabeth Chlipala l...@premierlab.com wrote: From: Elizabeth Chlipala l...@premierlab.com Subject: [Histonet] clostridium and bacterial spores in tissue sections To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Saturday, January 14, 2012, 12:58 PM Hello everyone I need a bit of help. I have to stain for clostridium and bacterial spores in tissue sections. I have come across a few references to staining these on smears but not on tissue sections. I did find one reference that used Ziehl-Neelsen to stain terminal spores from clostridium tetani. Does anyone out there know if the stain they use in microbiology for smears will work on tissue sections? Any advice would be appreciated. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.comhttp://www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] difficult cross section to cut
Hi Peggy 1. I have found that to cut bone the block has to be REALLY cold. Face block with increased knife angle as suggested by Rene, and then place in freezer overnight - then try sectioning 2. Try surface decalcification of your block overnight in whichever soln. you normally use. 3. Section at 4-6 microns 4. You do not say, but high profile disposables work better with bone than low profile 5, failing this, you could try dewaxing and re decalcifying for a few more days good luck On Tue, Jan 10, 2012 at 7:35 PM, DiCarlo, Margaret mdica...@kaleidahealth.org wrote: Histonetters, I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a femur that they think is osteoporotic. After decaling the cross section of the femur in 10% formic acid for 12 days, processing using xylene and embedding in Tissue Prep 2, I have been unsuccessful in attaining a section. The knife just skips over the bone. I have soaked the bone with a 50/50 solution of fabric softener and still can't get a section. Does anyone have any suggestions? Thank you. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 The Keeping You Informed section of Kaleida Health’s website features a wealth of information, stories and pictures about our valued workforce and about the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ist...@kaleidahealth.org or call (716) 859-. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] pouring of liquid nitrogen into a cryostat
bad idea - what about splash ups from the chamber? Very scary to contemplate On Fri, Oct 14, 2011 at 6:36 PM, Kaye Ryan kr...@nfderm.com wrote: Hi Everyone, Has anyone ever heard of pouring liquid nitrogen into the chamber of a cryostat that has warmed up to help cool it down more quickly? Does this do harm to the chamber or mechanisms of the cryostat? Any information would be appreciated. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Regarding cartilage and bone staining
Hi Pathik, perhaps you could give us some background on what it is you are researching? On Tue, Oct 11, 2011 at 11:01 PM, Kraniofacial Biology kraniofacialbiol...@gmail.com wrote: Hello Histonetters I am new to this site. I am a clinician and very fresh to research. I was doing whole mount cartilage and bone staining of whole head of mice and was surprised to see whole mouse head being used for experiment. I was wondering if there is any other staining method which can be used in place of the whole mount cartilage and bone staining that would stain the sections on a slide and works on cartilage and bone. This would also save the whole mouse to be used and many slides can also be used for other experiments. Regards Pathik ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] decal [sic] question
funny, I thought a decal was a sort of transfer for decorating things with - like guitars and hot rods - what others call stickers hard to trademark a word like that! On Tue, Oct 4, 2011 at 5:38 PM, Bob Richmond rsrichm...@gmail.com wrote: Bernice Frederick HTL (ASCP), Senior Research Tech at the Pathology Core Facility of the Robert. H. Lurie Cancer Center at Northwestern University in Chicago notes Ann Preece states acid decal uses aqueous solutions of either formic, nitric, or trichloroacetic acid. Other methods mentioned are Ion-exchange resin, electrical ionization and chelation. The histo bible! You've got to be almost as geezer as me to remember when Ann Preece's A Manual for Histologic Technicians was the histo bible. I was fortunate to be able to purloin a pristine (no stain spills) copy of the third edition (1972) from the wreckage of an old histology lab about 20 years ago. Indeed, Patsy Ruegg! Decal is a trademark of the Decal Chemical Corporation and should not be used generically for decalcifying solutions. See decal-bone.com Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ACD RNAscope
Hi all - is anyone using the above system for RNA detection in FFPE tissues - it sounds almost too good to be true? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] in situ for mRNA - refresher course?
Hi all, after more than a decade away from the bench I have been asked to start doing ISH on cryosections for BMPs and TGFbetas (mRNA) . I desperately need some guidance - even an offer to training me. i don't even know waht type of probes have been developed in the interim. I am sure I can get some funding for a couple of days overseas.. Of course, vendors with local representation are welcome to contact me. I look forward to a HUMUNGOUS response regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cadaveric bone
Hi all - a somewhat morbid question for the weekend. A student in the anatomy dept came to me with wax embedded samlpes of demineralized bone from dissection cadavers. The bone is very flinty and difficult to section. Is this perhaps due to the preseravation/embalming process that the bodies undergo? Is there something I can do to alleviate this problem prior to processing? I have tried dewaxing and furher demineralization, but the problem sems to be in the matrix itself-- BTW, using my usual protocol I ahv been able to section elephant tusk - so I think the prob is the bone rather than what I am doing to it best love haev a great weekend Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] protocol for in situ RT_PCR
hi could you add me on too pelase? On Mon, Aug 15, 2011 at 11:09 PM, Barone, Carol cbar...@nemours.org wrote: Anyone got a hot protocol for In situ RT-PCR? Haven't done one in 5 years (on my Perk and Elmer)...Know there must be some great new reagents and kits out there...opinions? Send to cbar...@nemours.org. Thx CB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] accidentally frozen samples
Probably the bone staining will be OK - how about sending the student to the arctic circle for a few days in winter? - that may be a good lesson about not freezing tissues. Just kidding :-) On Fri, May 20, 2011 at 9:24 AM, Tora Bardal tora.bar...@bio.ntnu.nowrote: One of our students accidentally put his samples in the freezer after formaldehyde/glutardialdehyde/PBS fixation. The samples (fish very early stage, of course rare species) were meant for LM/EM morphology studies. Good morphology is out, but is there a chance he can do cartilage/bone staining? Or do we need to send him abroad for new sampling at the next spawning time? Tora Bardal Senior Engineer NTNU Center of Fisheries and Aquaculture ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
could they possibly mean a H pylori? associated with gastric ulcers On Tue, May 10, 2011 at 3:47 PM, Rene J Buesa rjbu...@yahoo.com wrote: The so called reflux is a symptom of a gastric condition and, as such, cannot be detected by HC or IHC. Perhaps a gastric biopsy could reflect an anatomical alteration that accompanies it but cannot measure. Have your pathologists seriously consider this issue? René J. From: Dorothy Glass techman...@yahoo.com To: Histonet@lists.utsouthwestern.edu Sent: Monday, May 9, 2011 8:50 PM Subject: Re: [Histonet] (no subject) What is an IHC stain for gastric reflux. Pathologists are wanting to start using the procedure. Can anyone help? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Question about delayed start in Leica Tissue Processor
The easiest way to remember is to count the midnights between starting and ending - therefore today will be 0, tomorrow 1etc On Mon, Apr 18, 2011 at 6:24 PM, Jenny Vega histotech...@gmail.com wrote: Yes, that is what makes sense to me. Tomorrow morning after removing the specimens from the tissue basket I will check how my supervisor set the clock last Friday. If she chose 3-18:20, then by logic I will have to chose 4-18:20, since I will be away for the holidays for 4 days straight and I need the tissues to be processed by the next monday (april 25). I was told that the tissue processor starts at 6pm and it is done the next morning. I did not understood military time before and now I know that 18:20 means 6:20pm. Thanks On Mon, Apr 18, 2011 at 8:39 AM, Thomas, Nancy n...@stowers.org wrote: We have the same processor. If you are returning to work next Monday morning, then you would choose the 4 day delay. It would begin processing at 18:20 Sunday night and be ready on Monday morning. Nancy Thomas Stowers Institute -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Sunday, April 17, 2011 8:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about delayed start in Leica Tissue Processor Hello. I have little experience using the tissue processor from my lab which is an old version of the Leica TP1020. I was taugh that since on weekends the processor is not going to be used then you have to set up the days of delay for example 2-18:20 (2 represents saturday and sunday). On Monday (tomorrow) I have the day off, so the processor won't be used. When I come back on Tuesday I have to change the the time as 0-18:20 so that the processor starts working that day and on wednesday. On thursday, friday, saturday and Sunday will also have the days off. I am confused because my supevisor told me to set the time as 3-18:20 next Wednesday, and since I will not be working in the lab for 4 days straight that does not make sense, it is suppose to be 4-18:20 instead. I think she made a mistake telling me that since maybe she confused the program of this week when the processor was programmed as 3-18:20 because used for 3 days straight (saturday to monday). My supervisor won't be in the lab next week so I cannot ask her. This question seems silly but I want to be sure. I know that if tissue is left for a long time in paraffin the tissue can get over-harden and I don't want to program de delayed start as 3-18:20 thinking it is the correct way when is not. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] in situ buffer??
Greetings, Oh mighty histonetters! As per previous posts I am re-embarking on doing in situ hybridization. I will be using (hopefully) DIG labelled oligo probes, and ultimately visualizing with DAB. My question isare there a commercially available hybrizidation buffers? looking at the extensive list of ingredients it would be far easies (and cheaper) to get ready made stufff best regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] in situ - thanks 1 more query
Hi all, just wanted to say thanks for all the info received. L Looking through the protocols it appears that i need an anti-DIG antibody, preferably HRP labelled - any suggestions as to supplier? I remember using one from DAKO, but don't see it in the latest catalogue. Please - any sugestions as to where I can get it? Thanks again - you guys (and gals) rock!! -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] in situ methodology part 2
Hi all just as clarification .i need to brush up on my background of ISH - what new products/methododolgies there might be on the market. The tissues I will be using will either be rodent pups/embryos or formalin fixed/fresh adult tissue. As for the probe - thats a stick point - not enough background information to decide - but probably oligos. So...the questions still stand: - where can i find good on-line material - Who offers the best application manuals - is Roche still int he market? best regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] in situ hybridization
Hi all, after a decade of not doing ISH, I have been asked to initiate a project in our department. needless to say i am VERY rusty. can any one suggest: - a good on-line resource to brush up my knowledge - a company that have reference materials/handbooks etc (I seem to recall Roche did this)? - A course/workshop that i can attend specifically on embryo and bone in situ? As always i thank the forum for its help best regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cryojane slide residue
Hello alll! I am just starting to learn about cutting cryojane sections. I was given a sample of starfrost 4x and 5x as part of the installation. It is my understanding that these are moreadhesive than the ordinary snowcoat slides. HOWEVER, when i used these i noticed that there was a residue on the slide that stained up with eosin in a rather messy way. Is this normal? Can I get rid of it somehow? AlsoI am trying to cut sections of hydroxy apatite bone substitute on the cryostat. Even with the tape transfer method the implant disintigrates - is there any way to prevent this? looking forward to some answers best regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Slide Labeling System that Survives Citrate Boiling
long ago there was a cute system that worked with superfrost type slides - you know the ones that have the sort of coloured ends - etched through the paint to make a permanent marking Common HB pencil also survives on ordinary frosted slidesand surgipath pen marking esp if used on superfrost slides On 1/6/11, Cameron Nowell cameron.now...@ludwig.edu.au wrote: Hi List, I am looking for a slide labelling system to print labels for our research histology samples that will survive pretty much anything we throw at it. There seems to be lots of choices out there for chemical resistant labels but i can't seem to find much on ones that are resistant to antigen retrieval like citrate boiling. I have searched google and the histonet archives and the best i can find is a reference to General Data having some that may do the job. Does anyone out there have any more info or are you using someting that works well? Thanks Cam Cameron J. Nowell Microscopy Manager Centre for Advanced Microscopy Ludwig Institute for Cancer Research PO Box 2008 Royal Melbourne Hospital Victoria, 3050 AUSTRALIA Office: +61 3 9341 3155 Mobile: +61422882700 Fax: +61 3 9341 3104 Facility Website http://www.ludwig.edu.au/confocal/ This communication is intended only for the named recipient and may contain information that is confidential, legally privileged or subject to copyright; the Ludwig Institute for Cancer Research Ltd does not waive any rights if you have received this communication in error. The views expressed in this communication are those of the sender and do not necessarily reflect the views of the Ludwig Institute for Cancer Research Ltd. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Frozen section ...HELP
Hi all, after more than a decade of NOT cutting frozen sections, I find myself back at the ice-face. To get my hand in (not literally) I thought I would do some trial sections on stored tissue - stuff that was in formalin and now in 70% alcohol. Horror ; dismay. The tissue, once frozen, is all mushy in the middle. Is this because of teh long storage? is there anything I can do to improve the situation? much appreciated -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Nitroblue Tetrazolium Chloride
I used to do a stain on post mortem heart slices to look for recent infarctions. the entire tissue turned a sort of bluey colour which remained through formain fixation and processing. the tissue however was incubated in solution of NBT in a sodium cyanide containing buffer - not sure if that would still be allowed these days - but if you want - i could send you the protocol we used best regards On Thu, Oct 28, 2010 at 8:51 PM, Laurie Colbert laurie.colb...@huntingtonhospital.com wrote: I previously posted a question regarding Nitroblue Tetrazolium Chloride, but I didn't really receive the info that I needed - so I thought I would ask again. I need to purchase this item for a research doc. He wants to immerse tissue in this solution for 24 hours and then process as usual. It is my understanding that this is some kind of dye. I see that I can order it from Sigma in tablet form. I've seen it from other companies in a powder form. Has anyone ever used this reagent in the capacity that I describe? Is it available as a ready-to-use solution/liquid? Is there a certain strength/percentage that I should use? Thanks, Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] histomorphometry - clarified
Dear all, just to clarify issues here - i have a histomorphometry analysis system -a very nice one, and i sort of know how to use it - what i DON'T know is what sort of measurements i should be doing. So thanks to all who suggested systems, i'm sorry if my initial e-mail was not all that clear. further commentary on WHAT to measure and what parametres are useful will be welcomed best regards On Fri, Oct 1, 2010 at 12:12 PM, Katie McKinley katie.mckin...@i-path.co.uk wrote: Dear Louise, We have software which not only will allow the easy measurement of slides but also the management of your images. We specialize in whole slide scanning software, We can scan your slides and your images are saved on our network of servers and can be accessed online using secure login details. Because the software is online you can access your images from any location. The scan can be treated like a glass slide. You can zoom in to diagnostic resolution and pan around the whole slide and move through the layers. As well as measurements, you can annotate the slide, build up archives and banks of information and share with colleagues around the world instantly. We are currently in beta testing of our off the shelf imaging analysis software, but we do work on a consultancy basis to create analysis algorithms to suit our clients' needs specifically. Please visit our website for more info. www.i-path.co.uk Kind Regards Katie -Original Message- From: Wagner, Richard [BUL/LAK] [mailto:richard.wag...@buehler.com] Sent: 28 September 2010 21:48 To: louise.ren...@gmail.com Cc: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] histomorphometry Louise: Versatile image analysis software like OmniMet can solve this problem. Powerful digital image acquisition, object and field measurement options, and counting tools are standard features of the software. http://www.buehler.com/productinfo/biomedical/ia.htm Regards, Rick Wagner -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff Sent: Tuesday, September 28, 2010 8:36 AM To: louise renton Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] histomorphometry At the NSH this year, just this last Sunday in fact, Dr. Tony Villanueva demonstrated this very technique in his workshop. My best advice would be to contact him directly (avillanuev...@cox.net) and see what he can do for you. Another option would be to contact an image analysis vendor like BIOQUANT (nathan...@bioquant.com). Hope this information helps! Regards, Jack On Sep 28, 2010, at 3:25 AM, louise renton louise.ren...@gmail.com wrote: Hi all, I desperately need some advice from an experienced histomorphometristI am trying to translate the old cumbersome visual measurement of using an eypiece grid and doing point counting to an easier computer system - but I am not sure how to do it - any help out there? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Blade sharpening service
Delaware do ours and they're cheaper than leica On Mon, Sep 27, 2010 at 9:47 PM, Collette, Nicole M. collet...@llnl.govwrote: Hello, esteemed colleagues, I am in need of a service to have my 16cm tungsten carbide Profile D (a.k.a. Sweeney Todd) blades resharpened. Can anyone recommend a service that is relatively accessible to the West coast? I looked in the archives and couldn't find any recent posts, and my Googling has been unproductive. I'd rather not send them back to Leica in Germany to have them sharpened if I don't have to. Thanks for all your help! Sincerely, Nicole Collette UC Berkeley/Lawrence Livermore National Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] histomorphometry
Hi all, I desperately need some advice from an experienced histomorphometristI am trying to translate the old cumbersome visual measurement of using an eypiece grid and doing point counting to an easier computer system - but I am not sure how to do it - any help out there? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cutting Microarrays
hi, this sounds like the tissue was too cool when embedded in the wax - so it has not made a nice homogenous block that cuts as one. Can u re-embed? On Mon, Sep 20, 2010 at 10:54 AM, Rae Staskiewicz raest...@grics.netwrote: Good Morning All, I am having some issues when cutting a microarray containing 4mm punches. The punches are rolling and separating from the paraffin during cutting. Anyone have any tips or ideas?? Rae Staskiewicz MMCI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] filtering cytology stains
Hi - why not look at something like a millipore (millivex) syringe filter? This website has the application choice on it - or perhaps speak to your Millipore rep? http://www.millipore.com/techpublications/tech1/pb1951en00 On Wed, Sep 15, 2010 at 6:49 PM, Brandi Higgins brandihigg...@gmail.comwrote: Hello All, I was wondering how you are filtering your cytology stains. We are a relatively small lab, so we have been gravity filtering our cytology stains, but as we are getting busier with a larger volume of slides, especially fna's this is becoming a very time consuming process. I think vacuum filtration would be almost as lengthy a process. It was suggested that we look into getting some 200ml syringes that can come with an attached filter to suck in and pump out the fluid for faster filtration...is anyone using such a process? If so, do you have product names/numbers for the filters or the syringes. If anyone has another method they are using I would like to hear any suggestions. Thanks in advance for you input, Brandi Higgins, BS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] shrinkage
I seem to remember a good discussion in this in the following book:. L. P. Kok and M. E. Boon. *Microwave Cookbook for Microscopists*, Coulomb Press Leyden, Leiden (1992) p. 1–432 . Whetehr or not it is still available is another matter On Tue, Aug 24, 2010 at 5:17 PM, Edwards, Richard E. r...@leicester.ac.ukwrote: Anybody aware of the degree of shrinkage in paraffin processed tissues and/or GMA processed tissues?, many thanks. Cheers Richard Edwards Leicester University. Leicester U.K. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] how to make crashed ice?
Hi gudrun, Place the ice - ice cubes works best in a plastic bag, wrap in a towel and bash with a heavy object like a hammer. You can also use Jamie Oliver's trick - put ice cubes in a cloth tea towel, , bring the 4 corners together, tie them in knot, and then hit the parcel on the edge of your work surface until the ice is the sze you need hope this helps On Sat, Aug 7, 2010 at 12:12 PM, Gudrun Lang gu.l...@gmx.at wrote: Hi! I think this is a rather basic question ;-), but I'm looking for practical advice. We are going to try the OSNA-test for sentinel nodes. The application needs a pot with crashed ice while desintegrating the tissue with a mixer. So over the day this should be four to six litre ice, if we have to take fresh ice for each time, a group of sentinels has to be worked up. What is a practical way to make crashed ice in the lab? Thanks for your answers in advance! Gudrun ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] QC on stained slides
Thank you for your very helpful suggestionsThe situation here is a little weird as I am the only one doing the sectioning in this unit (research unit), and mostly i look at my own slides to do histomorphometry...so I have to grade myself??!!! Oh well, we do the best we can best regards On Mon, Aug 2, 2010 at 7:05 PM, WILLIAM DESALVO wdesalvo@hotmail.comwrote: Attached is a copy of the QA sheet that I provided at one of my NSH presentations. Review and use as needed. I believe your question of what makes a slide adequate or superior may be the wrong question. As long as you have a subjective review with a wide and varied specification, it will be extremely difficult to set a scoring process that will provide the desired feed back. I suggest you might want to approach in a different way and look at the number of defect or unacceptable products produced as compared to an agreed upon and high standard. use a Six Sigma tool to help you. I suggest you need an opportunities for improvement procedure and use the Defects per Million Opportunities (DPMO) tool. This will provide a process to evaluate the performance above and below standard in a simple and efficient manner. Your goal is never to deliver adequate work to your customer, the pathologist, but to always deliver the highest quality of work. You cannot improve the quality of work produced unless you know what is not meeting standard, not what is adequate or superior. Whether it is one person or multiple people working in the lab, there will always be variation in the product delivered because it is a manual process. You always want to reduce and narrow that variation to maintain the highest and consistent quality. But to narrow the variation you must have a standard and the standard must be agreed upon by the persons producing and the persons reviewing the work. I believe using a Six Sigma tool will provide you with the feedback you require and help you maintain the highest quality of slides delivered to the pathologists. Standardize your procedures and protocols, develop standards (highest quality standards) w/ your pathologists and then document, review, track and trend defects to improve the process. The data collected will give you specific information as to how you have performed to standard combined with the specific number of units produced by you (quality and quantity combined). This process will also allow you to compare multiple individuals working in the department and compare those individuals to each other. Everyone will be evaluated according to standards set for the work produced, plain, simple and effective. *William DeSalvo,* B.*S., HTL(ASCP)* * * Date: Mon, 2 Aug 2010 09:25:07 +0200 From: louise.ren...@gmail.com To: Histonet@lists.utsouthwestern.edu CC: Subject: [Histonet] QC on stained slides Hi all As part of a self assessment programme conducted by my employer, and related to my performance review and salary adjustment, I need to determine the criteria of what makes a stained slide acceptable or unacceptable. I was wondering if anyone out there had a checklist that they would be willing to share, that i could perhaps adapt. I realise that the easiest would be to send slides out for external control, but in this case it is not feasible. What I put together is this: - Quality of decalcification, processing, infiltration - Quality of sections (no wrinkles, missing bits, scores etc) - Entire representation of tissue area - staining pattern as expected according to protocol - coverslipped without bubbles or other inclusions - labelled neatly and correctly but, the question inmy mind is what would be the criteria that would make a slide merely adequate or truely outstanding? PLease help thank you -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] QC on stained slides
Hi all As part of a self assessment programme conducted by my employer, and related to my performance review and salary adjustment, I need to determine the criteria of what makes a stained slide acceptable or unacceptable. I was wondering if anyone out there had a checklist that they would be willing to share, that i could perhaps adapt. I realise that the easiest would be to send slides out for external control, but in this case it is not feasible. What I put together is this: - Quality of decalcification, processing, infiltration - Quality of sections (no wrinkles, missing bits, scores etc) - Entire representation of tissue area - staining pattern as expected according to protocol - coverslipped without bubbles or other inclusions - labelled neatly and correctly but, the question inmy mind is what would be the criteria that would make a slide merely adequate or truely outstanding? PLease help thank you -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] aanlktik8dnsqt16rpmss8_x5fgqqe_18-ctuenp71...@mail.gmail.com
My wrongI meant epidermis. Blame old age On Thu, May 20, 2010 at 12:08 AM, Andrew Burgeson nap...@siscom.net wrote: One thing i forgot to mention wasthat when you embed, try to orientate the tissue so that the long axis (if there is one) lies in the same direction as the cutting stroke. when embedding, orientate the tissue at a slight diagonal, so that the knife dous not continously pass through the tissue on the cutting stroke - (this works well for skins also, except make sure the dermis is away from the knife) I do not agree with the above statement about the dermis being embedded so as to be facing away from the blade. The last tissue to hit the knife edge should be EPIDERMIS. Dermis and SubQ fat should be the first tissues to hit the blade. Perhaps this is what you meant by dermis? Otherwise, I would agree with that methodology of orientation and angle. MethylMethacrylate bone embedding works very well from what I understand. See link: http://www.jhc.org/cgi/content/full/45/2/307 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] large fibrous bone tissue part 2
Dear Cornelia Jack i disagree about the soaking as this will negate the chilling effect of the freezer. The whole idea is to have the block as cold as possible to get maximum support from the wax*. If the tissue has been processed and fixed properly, there should not be a necessity for rehydrating on water One thing i forgot to mention wasthat when you embed, try to orientate the tissue so that the long axis (if there is one) lies in the same direction as the cutting stroke. when embedding, orientate the tissue at a slight diagonal, so that the knife dous not continously pass through the tissue on the cutting stroke - (this works well for skins also, except make sure the dermis is away from the knife) If this doesn't make sense, let me know and i will send a graphic privately *ease of sectioning relies on the embedding material being as close to the density/stiffness of the tissue being embedded. Thats why you can section undecal bone in resin best regards On Wed, May 19, 2010 at 7:33 AM, Jack Ratliff ratliffj...@hotmail.comwrote: Louise has very good advice here as related to paraffin processing of this tissue. I may even add to soak the block a little more before taking the final sections. However, have you ever thought of processing into MMA resin? If you have these capabilities you may be very pleased with the results and find the microtomy less problematic. Let me know if or how I can be of assistance! Jack On May 15, 2010, at 4:30 AM, louise renton louise.ren...@gmail.com wrote: I have found this helps. 1. Embed the tissue in a dep mould, as this provides more stability, then 2. Face the block 3.. leave in -20 deg freezer overnight 4. remove from freezer and cut sections 5. If you have multiple blocks to work with, leave them in the freezer until ready to cut regards On Fri, May 14, 2010 at 7:42 PM, Reuel Cornelia reuel.corne...@tsrh.org wrote: How do you process a fibrous bone tissue ( 7 mm thick). We have use Paraffin Type 9 from Richard allan Scientific to embed works well with our bone femur( 7 mm) when cutting but on fibrous bone it does not give us a good result in cutting the blocks. It is like cutting a uterus tissue but a little bit harder. Please give me your opinion on how to remedy this kind of tissue not mentioning double embedding method or plastic. Thank you. Reuel Cornelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fwd: TDE decalcifier, decalcification commentary
Thanks Gayle for your input. Trouble is, i like the TDE system, and despite not knowing what's in the solution the IHC results are great. I wasn't sure if endpoint testing could be used with other acids besides HCl, but you have answered my doubts on that score. I suspect that, from the smell, it is HCl, but I will have to dig out old chemistry books to remember how to determine an unknown acid! anyway, thanks again for your invaluable advice. best regards On Tue, May 18, 2010 at 8:52 PM, gayle callis gayle.cal...@bresnan.netwrote: Hi Teri and Louise, There is NO way to tell if a decalcifying solution is used up but you can be sure it IS used up. So the best thing to do is endpoint testing to know when the bone is free of calcium. All decalcifiers become exhausted, so one has to replenish the solution with fresh throughout the decal procedure, faithfully. This really doesn't take that much time in the long run, and guarantees properly decalcified bone that will not be overexposed to acid - a huge no no for decent staining. The weight loss/weight gain method is great if you don't have an xray machine. If the solution is an acid e.g. nitric, HCl, or formic, then one should do the chemical test or you can play with the weight loss/weight gain method. Attached are both the chemical and weight loss/weight gain methods, the latter was developed for testing nitric acid. However, this is the easiest method to test EDTA other than an xray machine, with the latter being the most sensitive i.e. accurate. *If the company cannot provide the answer for what is in TDE, then I would not use it*. MSDS sometimes tell you, but if not, that company would be sayonara from my lab. I have to know WHAT chemical is doing the work, very important to do IHC or routine only staining. I would rather make it up decalcifying solutions myself than buy an product that is unknown, and stubbornly proprietary! If you do a chemical endpoint test, do NOT stir the decal solution during decalcification. Suspend bones, then collect the aliquot from bottom of the container where Ca++ resides. Remove your samples, and rinse with tap water while you do the test. Return samples to FRESH decalcifying solution and proceed with decalcification/testing, etc, etc. May not be the answers you wanted but certainly how I have always done bone decalcification without problems. Take care, Ladies Gayle Callis *From:* Johnson, Teri [mailto:t...@stowers.org] *Sent:* Tuesday, May 18, 2010 9:58 AM *To:* 'gayle callis' *Subject:* Histonet post Hey Gayle, thought I'd point this one out to you. Looks like it's right up your alley. Message: 1 Date: Mon, 17 May 2010 19:30:30 +0200 From: louise renton louise.ren...@gmail.com Subject: [Histonet] TDE decalcifier To: histonet@lists.utsouthwestern.edu Message-ID: aanlktinrmd_lrg8otwuashunfc-unuv5x8fjviudy...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 hi all, although I have been using the TDE decalcification system for awhile, I was hoping to get some answers from the community. are there any thoughts as to what the solution is?. And how do you tell if the solution is used up or not. I wonder if anybody out there has some idea as to whether the solution is saturated with calcium salt or not. as always, I look forward to some answers. Best regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. I hope things are thawing a bit where you are. I wish it would quit raining and warm up here. Supposed to be 80 degrees this weekend. I can't wait! All the best, Teri __ Information from ESET Smart Security, version of virus signature database 5122 (20100517) __ The message was checked by ESET Smart Security. http://www.eset.com __ Information from ESET Smart Security, version of virus signature database 5122 (20100517) __ The message was checked by ESET Smart Security. http://www.eset.com -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending
[Histonet] TDE decalcifier
hi all, although I have been using the TDE decalcification system for awhile, I was hoping to get some answers from the community. are there any thoughts as to what the solution is?. And how do you tell if the solution is used up or not. I wonder if anybody out there has some idea as to whether the solution is saturated with calcium salt or not. as always, I look forward to some answers. Best regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] large fibrous bone tissue
I have found this helps. 1. Embed the tissue in a dep mould, as this provides more stability, then 2. Face the block 3.. leave in -20 deg freezer overnight 4. remove from freezer and cut sections 5. If you have multiple blocks to work with, leave them in the freezer until ready to cut regards On Fri, May 14, 2010 at 7:42 PM, Reuel Cornelia reuel.corne...@tsrh.orgwrote: How do you process a fibrous bone tissue ( 7 mm thick). We have use Paraffin Type 9 from Richard allan Scientific to embed works well with our bone femur( 7 mm) when cutting but on fibrous bone it does not give us a good result in cutting the blocks. It is like cutting a uterus tissue but a little bit harder. Please give me your opinion on how to remedy this kind of tissue not mentioning double embedding method or plastic. Thank you. Reuel Cornelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cryostat vs decal sections of bone
Hi all, last year sometime I was asked to budget for some equipment for our unit. Not expecting to get anything i aimed high, and requested a cryostat and cryoJane system. Lo and behold, the planetary influences were just right, and my request was approved. Now, I've got cold feet (pardon the pun) and I'm wondering if there *are* any distinct advantages of cryosections of bone over demineralised wax embedded samples in regards to immuno and in-situ ?. -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] retrieveing DNA from a mounted HE slide
Long, long ago when dinosaurs roamed and PCR was an emerging technique, what I would do is as follows: REmove coverslip in appropriate solvent, soaking te get any mounting media residue off. Rinse slide in abs alc and the 70% alcohol and then scrape off tissue into an Eppy with a new disposable blade. (Obviously, wearing gloves and taking care not to cut yourself). Rinse in sterile water, spin down, pur off supernatant , and contine as for normal extraction with your usual lysis buffer/extraction system. (back then we were using phenol chloroform!) The quality of the DNA is dependent on one of several factors, including the fixation medium, length of fixation, section thickness etc. On Mon, Apr 19, 2010 at 7:40 PM, Barone, Carol cbar...@nemours.org wrote: Hello histonetter's...I come one again to the experts in the field. I have been give two HE slides from an autopsy case (have no idea how old)...and have been asked to retieve this tissue into an eppy with lysis buffer for DNA. Does anyone out there have an existing method for doing this? I have a notion what is needed to do this (though I am somewhat skeptical on the quality of the DNA retieved from the tissue) .but, perfer not to re-invent the wheel...So if someone out there has a method for doing this, I would certainly appreciate it, if you wouldn't mind sharing it with us. Thanks... to all! CB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re:ub subscribe
Nah...I'd miss the myriad spelling variations variations of the word unsubscribe On Mon, Mar 22, 2010 at 10:35 PM, Geoff McAuliffe mcaul...@umdnj.eduwrote: Peter et al. All people have to do is read the instructions. How much simpler can one make it? Hit the delete key when you see any sort of unsubscribe message. Geoff Peter Carroll wrote: sometimes, i feel like im stuck in some histonet unsubscribe timewarp. i hate it! i really wish that the histonet admins would address this 'unsubscribe' madness once and for all. the periodic notices on how to unsubscribe arent working and sadly, the casual subscriber has proven that they dont know how to properly use a listserv... someone needs to rethink how this list is administrated. i mean no offense, just constructive criticism. if theres any way any of us can help out, im sure many of us with various expertises would gladly volunteer, just say the word! i am confident that i speak for all of us when i say that this repetitive, mindless unsubscribe business really detracts from the quality of my histonet experience, and theres no reason why we cant work together to fix it cone and for all :) - Original Message - From: Ms Janet Tao tao_ja...@yahoo.com Date: Monday, March 22, 2010 1:35 pm Subject: [Histonet] ub subscribe To: Histonet@lists.utsouthwestern.edu please unsubscribe me ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: Re[2]: [Histonet] help (UNCLASSIFIED)
Yep, I seem to remember reading somewhere that Silver nitrate was used as a
wart remedy to burn skin off...
still I feel for you - did the same thing once, and took a scrubbing brush
to my skin to get it all off!
2010/3/23 Maxim Peshkov maxim...@mail.ru
Patsy:
Not, I wrote chemical relations between these reagents.
Sorry, but I am not recommended it for your skin.
Really, nothing necessary to do.
After some days old keratin will fall off and
you will not see any traces of silver nitrate.
Sincerely,
Maxim.
You wrote at 23 March 2010, 21:33:43:
Are you trying to kill me?
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pru...@ihctech.net
web site www.ihctech.net
This email is confidential and intended solely for the use of the
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-Original Message-
From: Maxim Peshkov [mailto:maxim...@mail.ru]
Sent: Tuesday, March 23, 2010 11:43 AM
To: pru...@ihctech.net
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] help (UNCLASSIFIED)
Patsy:
Chemically, silver nitrate deposites can be
reduced onto the slides by 0.5% potassium ferricyanide.
I am not sure that it may work onto living skin.
Sincerely,
Maxim Peshkov,
Russia,
Taganrog.
mailto:maxim...@mail.ru
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Это сообщение проверено Антивирусной системой NOD32.
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С уважением,
Maxim mailto:maxim...@mail.ru
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Bone Research Unit
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South Africa
+27 11 717 2298 (tel fax)
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There are nights when the wolves are silent and only the moon howls.
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
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Re: [Histonet] Fire in the lab
hey, I once set my hair on fire in the lab...singed off my eyebrows and burnt my nostril hair. It took several days for the burnt hair smell to get out of my nose!!. This too was in a time long long ago in another time dimension On Sat, Feb 27, 2010 at 12:51 AM, Joe Nocito jnoc...@satx.rr.com wrote: Once upon a time in a far away land, we used to boil our embedding molds in boiling soapy water, over an open Bunsen burner, followed by an alcohol rinse then air dry. One time the fire alarm was activated and we had to evacuate the hospital. We were out there quit awhile. When we received the all clear to go back into the hospital, I was the first one back in the lab and the fire department was there, looking into our pot that had boiled out and was smoking up the lab. This wasn't the cause of the first alarm, but it did set off the second. Joe - Original Message - From: CHRISTIE GOWAN christiego...@msn.com To: histonet@lists.utsouthwestern.edu Sent: Friday, February 26, 2010 8:20 AM Subject: [Histonet] Fire in the lab Dear Histonet Friends, I just wanted to share an incident we recently had with an old paraffin pot. One of my techs came in on Sunday to embed some tissues, went into the processor room and smelled something burning. He noticed our old paraffin pot had charred looking labels on the outside so he went over, opened the lid and poof!!! the pot went up in flames. The thermostat had gone haywire and heated the paraffin to flash point. Opening the lid gave it the oxygen it needed to ignite. He triggered the alarm, made the appropriate call and then put it out with an extinguisher. Of course it kept re-igniting because he could not get behind it to pull the plug. The fire dept finally was able to get it pulled out and unplugged. Needless to say the tech was shaken and the room was a mess. I applaud his courage and am not sure I would have done the same. There was enough xylene and alcohol on the 4 processors to cause quite an explosion but everything else was in a flammable cabinet. I was wondering if this type of thing had ever happened to anyone else?? Needless to say, we have de-comissioned all old paraffin pots and will order only those with over temp safety features. I guess I just wanted to remind everyone that fires can happen in the lab and do probably more often than we hear about. This was the first time for me and I have been in this business for over 20 years. Take care and be safe. Christie Gowan HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] problem with subbed slides
Just for interest's sake could this be some fungal growth? Try staining a slide with H/E and look under the microscope. how to fix? perhaps acid digestion and resubbing? On Wed, Feb 10, 2010 at 8:15 PM, Hisham Mohammed hisham@gmail.comwrote: dear histonetters following gelatin subbing of big glass slides we observed numerous white patches in almost the entire batch of slides subbed. what could be the remedy to remove these white patches. waiting for a practical solution. with regards hisham mohammed senior research centre national brain research centre ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Alternatives to BioQuant for Bone Histomorphometry
I use a system called analySIS (for Windows)- marketed here by Olympus. We use this for percentage bone calculations, linear measurements and touch counting. The data is immediately put into a spread sheet and you have an automatic stats facility On Mon, Feb 8, 2010 at 2:21 PM, Kathleen Jones kjo...@upei.ca wrote: Hi Adam Have you checked out ImageJ? It's a free downloadable program that I have used for alveolar morphometry. Fairly user friendly, more so than BioQuant, although not quite as thorough. Good Luck Kathy Kathleen Jones Research Technician Pathology/Microbiology AVC - UPEI (902)566-0595 Adam . anonwu...@gmail.com 2/5/2010 6:38 PM Hi all, I am looking for an alternative program to BioQuant for bone histomorphometry. We need to quantify the number of osteoblasts / osteoclasts per bone surface area as well as the percent surface area occupied by those cells. We have a computer with BioQuant on it available, but we find the software to be incredibly clunky and often nearly impossible to use. Based on my limited attempts to use it, it very well might rank as one of the worst user interfaces I've ever seen, and I was trained in computer science and have seen my fair share of horrible software (I'm looking at you, Lotus Notes). Anyhow, any suggestions on a (preferably cheap / free) replacement for doing simple analysis or how to make BioQuant less painful would be very helpful. Thanks, Adam ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] paper on osteoclasts
Hi all, I am going crazy - and its not even friday yet! A little while ago (OK about 3 years) i came across a paper where the researcher had shown the transition of macrophages to osteoclasts using immuno staining - i cannot seem to find it on Pubmed, dont know the author date or journal. i hope soemone out there can help best egards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] OT - adoptive t cell therapy
Hi all, this is a very OT subject, but does anyone know about the above therapy in regards to adenocarcinoma? (i know that trialls are being done on melanomas presently). i would be grateful for any info -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cutting paraffin sections in a warm room
One of the little things I learned along the way regarding bone sectioning: if time allows, trim (face) the blocks and leave them in the deep freeze (-?20) overnight. T Then when you are ready to section, take ONE block out at a time and place on ice. this trick is especially useful for us as we cut 50 serial sections at one go. The block is usually cold enough to get at least 40 sections off it before recooling. BTW, we use the deepe embedding moulds, about 1cm deep, so if you are using the flatter mouds, maybe overnight is not necessary my 2c worth have a great weekend! On Thu, Nov 5, 2009 at 8:10 PM, Adam . anonwu...@gmail.com wrote: Hi all, I've just recently started cutting paraffin sections (of bones). A few weeks ago, the facilities people decided that it was fall and now the lab is significantly warmer than it used to be, and my paraffin is falling apart and sticking to everything. Apparently, we can't control the temperature through a thermostat at all, and the microtome is inside the lab. I was wondering if anyone had any ideas on how to section in a warm room. Thanks, Adam ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Sakura TDE 30
OK, Firstly, what you do depends on what samples you are dealing with. We decalcify smallish samples of bone about 1x1cm - this can either compact or trabecular bone. Here are some of the things I learned.. 1. Do not overload the chamber.place samples between the electrodes, and rather do fewer samples - decal can be very quick if used right. 2. Do not use metal lids on casettes if using them (or any metal clips, parts etc) 3. If the samples aer heavily mineralised, ie huge pieces of compact bone, change the fluid when you see that the fizzing around the electrodes has slowed 4. Rather swich off the unit overnight (with the samples still in) if you are doing a long decal - then swich on in the day, so that you can monitor progress. 5. Not all sam[les will decalcify at the same rate - check them all individually at 4 - 5hr, or in the morning of the next day 5. When decal is finished - rinse samples thoroughly in running tap water for at LEAST and hour before processing 6. I have decalcified a slice of pig femur about 0.5 cm thick in about 5hrs hope this helps On Thu, Oct 1, 2009 at 7:48 PM, Isabel Cristina Soares Brandao isabel.bran...@sarah.br wrote: The time expended in the process was too long and the tissues had artifacts when examined on the microscope. We did exactly the same procedure described in the Sakura brochure. Do you do something different? How do you verify the process? How long does it take to get the samples decalcified? Thanks for your help! -- De: histonet-boun...@lists.utsouthwestern.edu[ SMTP:histonet-boun...@lists.utsouthwestern.edusmtp%3ahistonet-boun...@lists.utsouthwestern.edu] em nome de louise renton[SMTP:louise.ren...@gmail.comsmtp%3alouise.ren...@gmail.com ] Enviada: quinta-feira, 1 de outubro de 2009 14:35 Para: Histonet@lists.utsouthwestern.edu Assunto: Re: [Histonet] Sakura TDE 30 Could you please explain what the problems are. I have had excellent results with this system On 10/1/09, Isabel Cristina Soares Brandao isabel.bran...@sarah.br wrote: Hi, Does anyone out there have experience with the decalcifier System from Sakura? We have just recived the equipment but we could'nt get the results that they describe in the brochure. Isabel C.S. Brandão Associação das Pioneiras Sociais Patologia Cirúrgica - Brasília - Brasil ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Sakura TDE 30
Could you please explain what the problems are. I have had excellent results with this system On 10/1/09, Isabel Cristina Soares Brandao isabel.bran...@sarah.br wrote: Hi, Does anyone out there have experience with the decalcifier System from Sakura? We have just recived the equipment but we could'nt get the results that they describe in the brochure. Isabel C.S. Brandão Associação das Pioneiras Sociais Patologia Cirúrgica - Brasília - Brasil ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] BLTS - OT
Hey, I thought you meant that famous sandwich ...Bacon Lettuce and Tomato - guess it must be lunchtime On Tue, Sep 29, 2009 at 3:20 PM, Kathleen Jones kjo...@upei.ca wrote: Hello Histonet I am wondering if anyone out there is familiar with the Barbeito-Lopez Trichrome stain. I am using it to identify early myocardial infarction. My damaged tissue is showing up, but it is very subtle. The papers I've read show the necrotic myofibers 'popping' out as yellow, whereas mine are more pale, almost pink. I'm not sure if I am under decolorizing or if I should leave it in the molybdic acid aniline blue methyl orange longer. Any suggestions would be greatly appreciated! Kathy Jones Research Technician AVC-UPEI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] mice legs
No, I tend to use my own for work!sorry couldn'r resist midweek madness...;-) On Tue, Sep 15, 2009 at 3:42 PM, Shaw, Sharon shs...@wpi.edu wrote: Good Morning Histo World, I would like to know if anyone is working with mice legs, I have a PI that I work with that wants to process the whole leg, the problem is I need to decal it first and is wondering if the decal will break down the tissue, I think it would he doesn't think so. And if anyone has do this would it be possible to share your protocol with me from decal to processing. Thanks, Sharon- WPI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Help With Hemo Fading
Even long term exposure to indoor lighting (fluorescent type) will fade sectiions. We work in a building where ther lights are permanently on. If we want to keep our sections fresh and helathy, they get covered up as soon as . best regards On Fri, Jul 3, 2009 at 9:09 AM, Kemlo Rogerson kemlo.roger...@waht.swest.nhs.uk wrote: I agree.. Also you're not storing them in sunlight are you? Silly question I know. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 02 July 2009 20:28 To: histonet@lists.utsouthwestern.edu; sr...@aol.com Subject: Re: [Histonet] Help With Hemo Fading The fading most probably is caused by acid in the permanent slide, probably because the sections were passed through the alcohols very quickly after the acid differentiation, or they stayed little time in tap water after differentiation or no bluing agent was used. It is unlikely that the mounting medium is acidic, although that could also be the cause also. An acid environment over the cover slipped section is the most probable culprit for the henatoxylin fading. Check the staining protocol. René J. --- On Thu, 7/2/09, sr...@aol.com sr...@aol.com wrote: From: sr...@aol.com sr...@aol.com Subject: [Histonet] Help With Hemo Fading To: histonet@lists.utsouthwestern.edu Date: Thursday, July 2, 2009, 2:42 PM Hello everyone, ? We are having problems with short-term hematoxylin fading and loss of detail. The pathologist is freaking out! I've seen hemo fade over a long period of time but not in a matter of a few months. Slides from one year ago are really bad. ? I've been out of the business for a number of years and in the interim much has changed including reagents. These are GI tract biopsies processed by microwave. ? Any thoughts at all? ? Thanks! Marg ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Floor materials
Vinyl floors can be almost lethally slippery if wax gets on them its ok if staff wear flat shoes but woe betide someone coming in with heels! On 4/28/09, Feher, Stephen sfe...@cmc-nh.org wrote: We're building a pathology lab and we're at project phase where flooring materials is being discussed. The initial choice of the architect is to use sheet vinyl flooring in the working areas of the lab (with the exception of the morgue). I'm not particularly impressed with the way vinyl flooring stands up to stains, solvents and wax. Do any of you have suggestions or experience in a type of flooring for the path lab that is superior to commercial vinyl? Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfe...@cmc-nh.org mailto:sfe...@cmc-nh.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] processing v-e-r-y tiny samples
Shandon - or whatever they're called this week- sell nylon mesh biopsy bags. These are flat bags, sealed on 3 sides. I have found that if you cut one corner off (ie, a oblong piece with 2 sides still sealed) just a tiny bit bigger than the cassette, you can open the bag, like a book, insert the sample and flatten the bag over the specimen and place it snug and flat in the bottom of the casette. It sems to stay like that through processing (esp if kept the cassette is kept flat in the basket), and is easy to locate against the mesh for embedding. On 3/19/09, Andrea Grantham algra...@u.arizona.edu wrote: Good Morning! In keeping with the weirdness of the projects I get in this lab today my question is about processing mosquito GI tracts. I have a processing schedule - that is not the problem. I'm wondering if anybody out in histoland has a suggestion for what kind of cassette to use. I was thinking of the histoscreen cassette because these GI tracts are so thin (I think thinner than a hair)and I don't want to wrap them or use sponges because I'm afraid that I'll loose them or crush them. Any ideas? Andi . : Andrea Grantham, HT(ASCP) Dept. of Cell Biology Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415)Tucson, AZ 85724-5044USA : : (FAX: 520-626-2097) (email: algra...@u.arizona.edu) : :...: http://www.cba.arizona.edu/histology-lab.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] 20 micron resin sections
Thanks all, This is more or less what i thought, that 20mu sections would prove to be difficult and not all that feasible. So, I wait with bated breath for the protocol from the researcher On 3/13/09, Peggy Bisher mbis...@princeton.edu wrote: One of the labs here use JB4 to section Zebrafish. I sent your question to them to see if they could help you out. Here is their response: The consensus in the lab (Kari and I) is that no, probably not. The size would probably shred the section and chip it to where they would be uneven, etc. Probably cryo or vibratome would be best. They routinely cut their sections between 2-5 microns. Good luck to you! Cheers, Margaret E. Bisher Electron Microscopy Histology Core Facility Manager Department of Molecular Biology Princeton University Moffett Laboratory, Room 113 Princeton, New Jersey Office: (609) 258-7026 Fax: (609) 258-8468 mbis...@princeton.edu On 3/13/09 3:53 AM, louise renton louise.ren...@gmail.com wrote: Hi all, I have a query from a colleague doing research on neuroanatomy as to whether it is possible ( with relative ease) to cut 20mu sections from JB4 resin embedded tissue? Apparently these sections ae to be stained and then used for stereomicroscopy. My experience is not that extensive to be able to answer her, so I would appreciate some advice here best regards-- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 20 micron resin sections
Hi all, I have a query from a colleague doing research on neuroanatomy as to whether it is possible ( with relative ease) to cut 20mu sections from JB4 resin embedded tissue? Apparently these sections ae to be stained and then used for stereomicroscopy. My experience is not that extensive to be able to answer her, so I would appreciate some advice here best regards-- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] thanks
thanks to all who replied to my query regarding slide filing pages - I have lots of browsing to do today - yay!!! have a great weekend! -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] slidex filing pages
Hi all, Anyone familiar with Slidex pages - rigid pastic pages in which one can store slides (20/page)? These pages are pre-punched so they can go into ring binder but there is also a filing box that one can use. We have been using these for years, but the agency that dealt with the parent company has pulled out and we are getting no joy from Slidex Japan. Seems they don't answer English correspondence. Any thoughts?-- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] lids for stainer containers
These sound like the kind of thing that goes on a foil take away or freezer container. Try a home suply depot? We have specialist stores here that supply serious home bakers and chocolatiers and I know that they carry what you describe - bit far for you to come though... On 12/3/08, Perry, Margaret [EMAIL PROTECTED] wrote: We are looking for a source that sells a lid that looks like wax covered cardboard. We use them on top of the Shandon slide containers holding xylene or formula 83 while waiting to coverslip. They are about 5x7 and very thin. They have been here for years so I have no idea where they came from. Any help in finding them or a substitute will be greatly appreciated. Thanks. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] osteoid
Thnaks for all the replies I got on this subject - they have clarified my thoughts somewhat -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Osteoid identifiction
Hi al, I have received a sample of ?bone where the clinician wants to identfy osteoid. According to my knowledge (now perhaps a little daed) the traditional processing method is plastic embedding of some sort. Is this still the case or are there methods to use standars paraffin processing? Best regards-- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tunnel vs TUNEL
Thanks to those who answered, those who were serious and those who weren't :-) Now i can sleep easier, nights. Anyway its all the fault of spellcheckers (which is satining got in..to those who picked it up!) love u all On 10/15/08, Jacqui Detmar [EMAIL PROTECTED] wrote: Hey there. I'm in the cell death field and do *plenty* of TUNELs. The short form you are using is correct. I have also seen it as terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling. Anyway, Tunnel is dead wrongsomeone just made a mistake. I am shocked to learn that even reputable journals are prone to these grin, wink. Jacqui Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 25 Orde Street, room 6-1001 AJ Toronto, ON, Canada M5T 3H7 Tel: 416-586-4800 x5607 Fax: 416-586-8588 email: [EMAIL PROTECTED] -- *From:* [EMAIL PROTECTED] on behalf of louise renton *Sent:* Wed 10/15/2008 4:03 AM *To:* Histonet@lists.utsouthwestern.edu *Subject:* [Histonet] Tunnel vs TUNEL hey guys, what is the correct way of writing this? I always thought it was TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling ), but I recently saw an article in a reputable journal refer to tunnel satining - is ths something differnt or just sloppiness? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tunnel vs TUNEL
hey guys, what is the correct way of writing this? I always thought it was TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling ), but I recently saw an article in a reputable journal refer to tunnel satining - is ths something differnt or just sloppiness? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
