Re: [Histonet] Histonet Digest, Vol 234, Issue 10

[Patsy Ruegg via Histonet]

Aren't they going to offend the mail histotechs with that name? Just kidding.


Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
39037 N 11th Ave
Phoenix, AZ 85086
C 720-281-5406
prueg...@hotmail.com
Doug Ruegg
C720-281-5407
douglas...@hotmail.com


From: histonet-requ...@lists.utsouthwestern.edu 

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To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 234, Issue 10

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Re: [Histonet] Histonet Digest, Vol 233, Issue 7

[Patsy Ruegg via Histonet]

this is a strange post on histonet

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
39037 N 11th Ave
Phoenix, AZ 85086
C 720-281-5406
prueg...@hotmail.com
Doug Ruegg
C720-281-5407
douglas...@hotmail.com


From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Wednesday, April 19, 2023 11:00 AM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 233, Issue 7

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Re: [Histonet] Histonet Digest, Vol 223, Issue 14

[Patsy Ruegg via Histonet]

I used tonsil as + control.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
39037 N 11th Ave
Phoenix, AZ 85086
C 720-281-5406
prueg...@hotmail.com
Doug Ruegg
C720-281-5407
douglas...@hotmail.com


From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Friday, June 24, 2022 11:00 AM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 223, Issue 14

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Re: [Histonet] Histonet Digest, Vol 213, Issue 22

[Patsy Ruegg via Histonet]

I would suggest using something like histogel or agar to make layers of blood 
drops in a tube, then freeze them and make frozen sections. Sounds tricky.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
39037 N 11th Ave
Phoenix, AZ 85086
C 720-281-5406
prueg...@hotmail.com
Doug Ruegg
C720-281-5407
douglas...@hotmail.com


From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Tuesday, August 31, 2021 11:00 AM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 213, Issue 22

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Re: [Histonet] Histonet Digest, Vol 211, Issue 7

[Patsy Ruegg via Histonet]

The blue stain from prussian blue is a chemical reaction and indicates that 
there is a lot of iron in the section, you would not want it not to stain some 
of the iron there would you? I agree with John, take an adjacent section for a 
general stain.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
39037 N 11th Ave
Phoenix, AZ 85086
C 720-281-5406
prueg...@hotmail.com
Doug Ruegg
C720-281-5407
douglas...@hotmail.com


From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Monday, June 7, 2021 11:00 AM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 211, Issue 7

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Re: [Histonet] Water under sections

[Patsy Ruegg via Histonet]

This is all good advise. I always picked up section and tapped the slide on 
counter and then dried standing up. This is really a problem if you are at all 
above 5K feet in altitude where water boils violently at 92-93dc, which will 
destroy your tissue section. I have written a couple of papers about on this.


From: Greg Dobbin 
Sent: Friday, January 22, 2021 11:45 AM
To: b-freder...@northwestern.edu 
Cc: histonet@lists.utsouthwestern.edu 
Subject: Re: [Histonet] Water under sections

Hi Bernice,
In my lab, water under the sections is unique to charged slides. And you
are correct, if there is water under the section when the slides are heated
for antigen retrieval, the boiling (or at least very hot) water will damage
or entirely destroy the section.

We allow the charged slides to drain (upright) for a few minutes and then
carefully make a hole in the edge of the wax and use a Kimwipe or paper
towel to carefully wick the excess water out as much as we can without
touching the tissue section. Then we "flick" or shake sharply to remove any
residual water that may remain before we bake them (FYI, we choose not to
bake on the stainer). If after baking 30 mins at 60C, we notice water
(maybe someone was not as diligent earlier?), we wick away the excess and
bake for another 15 mins to ensure good adhesion of the section to the
slide before proceeding to the immunostainer. Hope this helps.
Greg

--
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE  C0A 1P0


*Everything in moderation...even moderation itself**!*

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Re: [Histonet] Microtome at home

[Patsy Ruegg via Histonet]

I have done it, but you are right, I had my own private business, not sure why 
it would be a problem, especially for research.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: Jamie Watson 
Sent: Wednesday, April 15, 2020 5:44 PM
To: Histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Microtome at home

Hello all,

Our pathologist has come up with the idea of sending a microtome and waterbath 
home to someone that cannot come to work due to COVID 19.  We are a research 
lab and work with mouse and rat tissue.  Does anyone know of any issues with 
doing this?  I have never heard of anyone cutting slides at home other than 
someone with a private business.

Thank you.

Jamie

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Re: [Histonet] FW: Microtome at home

[Patsy Ruegg via Histonet]

I agree with this point and as far as clocking in and out, I would think you 
could work out something like getting paid piece mill, perhaps charge per slide 
or block cut, that way you could do it on your own time and not have to clock 
in.


Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: Joseph Saby 
Sent: Thursday, April 16, 2020 8:03 AM
To: Porter, Amy ; Porter, Amy via Histonet 
; histonet@lists.utsouthwestern.edu 
; Steven Crochiere 
Subject: Re: [Histonet] FW: Microtome at home


You will need to make sure all pertinent SOPs and EOPs are followed, as well as 
all safety guidelines/protocols. Just because it is not human tissue doesn't 
mean that it can't have its share of nasties.
Joe Saby

Sent from Yahoo Mail on Android

  On Thu, Apr 16, 2020 at 8:21 AM, Porter, Amy via 
Histonet wrote:   Make sure of insurance 
coverage and safety for the employee and that they are covered in case of 
injury - are they still clocking in and out in some fashion. just thinking 
in a bigger box.


From: Steven Crochiere via Histonet 
Sent: Thursday, April 16, 2020 6:36 AM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] FW: Microtome at home

Jaime,

I don't see a problem with a research setting. If it was patient care, CLIA 
would need to inspect the set up in the person home. The same goes for our 
pathologists who read slide at home.

Steve

-Original Message-
From: raestask via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, April 15, 2020 7:51 PM
To: Jamie Watson ; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome at home

I wouldn't think there would be any problem.Rae Staskiewicz HT(ASCP)Sent from 
my Verizon, Samsung Galaxy smartphone
 Original message From: Jamie Watson via Histonet 
 Date: 4/15/20  6:44 PM  (GMT-06:00) To: 
Histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome at home Hello 
all,Our pathologist has come up with the idea of sending a microtome and 
waterbath home to someone that cannot come to work due to COVID 19.  We are a 
research lab and work with mouse and rat tissue.  Does anyone know of any 
issues with doing this?  I have never heard of anyone cutting slides at home 
other than someone with a private business.Thank 
you.Jamie___Histonet mailing 
listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Re: [Histonet] Need a procedure

[Patsy Ruegg via Histonet]

From: John Garratt 
Sent: Thursday, January 23, 2020 10:51 AM
To: Terri Braud 
Cc: histonet@lists.utsouthwestern.edu 
Subject: Re: [Histonet] Need a procedure

Hi Terri, I suggest you use Histogel for block preparation. It works 
exceptionally well, it is good for IHC and does not have the pitfalls of 
plasma/thrombin.
Plasma/thrombin does work well for cell blocks but you will have to consider an 
ethical and safe source for your plasma.
The instructions for using Histogel are in the package insert though I have one 
comment. Be careful how you warm the Histogel and use a heat block. Do NOT use 
a microwave since there is a tendency to overheat the gel and you will end up 
with poor quality IHC.

John


www.cpqa.ca

‐‐‐ Original Message ‐‐‐
On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet 
 wrote:

> Hi fellow Histonetters - I'm in need of some help, please
> Background - We currently use agar to capture our scant cell blocks for 
> processing. I am unfamiliar with the Plasma/Thrombin method of cell block 
> preparation and am interested in comparing it to our current method
> Request - Could you please send me your procedures for this method, 
> specifically where you purchase your plasma and thrombin and what species are 
> used?
> Thanks in advance. Histotechs rock!
>
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
>
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet




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Re: [Histonet] Histonet Digest, Vol 195, Issue 10

[Patsy Ruegg via Histonet]

I never minded being called in to assist for an aspect of transplants at the U, 
I figured I was helping a very needy patient.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com


From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Thursday, February 13, 2020 11:00 AM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 195, Issue 10

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
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Re: [Histonet] Need a procedure

[Patsy Ruegg via Histonet]

Terri, when I was in animal research we used the blood from the animal to make 
plasma and combined it with some thrombin from the hospital pharmacy.  Of 
course that is tedious especially since histogel is available.  I warmed the 
histogel by placing a tube of it in a beaker of hot water, do not mw it.


Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: John Garratt 
Sent: Thursday, January 23, 2020 10:51 AM
To: Terri Braud 
Cc: histonet@lists.utsouthwestern.edu 
Subject: Re: [Histonet] Need a procedure

Hi Terri, I suggest you use Histogel for block preparation. It works 
exceptionally well, it is good for IHC and does not have the pitfalls of 
plasma/thrombin.
Plasma/thrombin does work well for cell blocks but you will have to consider an 
ethical and safe source for your plasma.
The instructions for using Histogel are in the package insert though I have one 
comment. Be careful how you warm the Histogel and use a heat block. Do NOT use 
a microwave since there is a tendency to overheat the gel and you will end up 
with poor quality IHC.

John


www.cpqa.ca<http://www.cpqa.ca>

‐‐‐ Original Message ‐‐‐
On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet 
 wrote:

> Hi fellow Histonetters - I'm in need of some help, please
> Background - We currently use agar to capture our scant cell blocks for 
> processing. I am unfamiliar with the Plasma/Thrombin method of cell block 
> preparation and am interested in comparing it to our current method
> Request - Could you please send me your procedures for this method, 
> specifically where you purchase your plasma and thrombin and what species are 
> used?
> Thanks in advance. Histotechs rock!
>
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
>
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet




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Re: [Histonet] Frozen Gel Pack with paraffin block for shipping?

[Patsy Ruegg via Histonet]

whether I used an ice pack or not, I always bagged each block in it's own 
individual container/plastic bag, etc., just in case there was melting the 
tissue could possibly be recovered.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: Paula 
Sent: Wednesday, March 6, 2019 9:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Frozen Gel Pack with paraffin block for shipping?

Hello,



This question has come up here at work a few times. Apparently, the
department does different protocols, depending on who is doing the job of
shipping out a paraffin block for additional testing. Our transcription
department handles this and I was asked to look into it so I can submit a
protocol for them to follow.



Is there an industry standard to follow?  I'm leaning towards always putting
in an frozen gel pack during the warmer months inside the shipping container
to avoid any melted blocks. I know the melting point is somewhere around
136F (58C), but I still want to ensure the block doesn't get warped or
altered in any way because of the heat. Plus, we shouldn't have to check the
weather before sending out and I think it  just should be our standard
protocol.



What are your thoughts and if there is a standard, please share that with
me.



Thank you very much,

Paula

Lab Manager


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Re: [Histonet] Histonet Digest, Vol 184, Issue 1

[Patsy Ruegg via Histonet]

here here

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: Joseph Saby 
Sent: Tuesday, March 5, 2019 9:47 AM
To: Jordan, Kelley; Mark Tarango
Cc: HistoNet
Subject: Re: [Histonet] Histonet Digest, Vol 184, Issue 1

The more important question is who the h*ll is Kelly Jordan.
Terri has established her competency and reputation in NSH and the Histonet for 
probably over 20 years.
You have done your company a great disservice.
People will remember you, probably not as you would wish.
Joe Saby, retired


On Tuesday, March 5, 2019, 11:42:30 AM EST, Mark Tarango via Histonet 
 wrote:

 How about passing on to the department that could fix the issue?  Where's
the empowering innovation?

On Fri, Mar 1, 2019 at 10:35 AM Jordan, Kelley via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> More bad press in histonet...not sure if we should pass it on to
> Marketing??
>
> @Will, Who as Teri Blaud at  Holy Redeemer Hospital ? Teri is always
> always bashing us.
> Kelley Jordan
>
> Strategic Account Manager - SC, NC, TN and KY
>
>
> A Member of the Roche Group
>
> Ventana Medical Systems
>
> Mobile:  803.504.1135
> Customer/Technical Support: 1.800.227.2155
>
> kelley.jor...@roche.com
>
> www.ventana.com<http://www.ventana.com>
>
>
>
> Empowering | Innovation
>
>
> Confidentiality Note: This message is intended only for the use of the
> named recipient(s) and may contain confidential and/or proprietary
> information. If you are not the intended recipient, please contact the
> sender and delete this message. Any unauthorized use of the information
> contained in this message is prohibited.
>
> histonet <http://lists.utsouthwestern.edu/mailman/listinfo/histonet>
>
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Re: [Histonet] Histonet Digest, Vol 184, Issue 1

[Patsy Ruegg via Histonet]

Maybe that is the trouble, they keep passing these technical issues on to 
Marketing instead of R&D.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com

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Re: [Histonet] New York Qualifications for Histology Supervisor

[Patsy Ruegg via Histonet]

In my opinion this issue is related to the fact that HT's are not required to 
have BS degrees, like Med Techs and Cytotechs, and that clia/cap does not 
require ASCP certification to work in a histology lab.  We have always been the 
red headed step child in the lab because of this.  This is why NSH has fought 
so hard to at least require the AS degree with certain science credits.  As far 
as I know, unless it has changed CLIA doesn't even require that those working 
in histology be ASCP certified.  I may be behind on that rule???  I know many 
very qualified folks who are stars in histology who do not have a BS degree, 
but I think those times are behind us, as sheep skin seems to count for more 
than experience in this new world.  It is a shame because that sheep skin does 
not a good Histotech make, on it's own.

Patsy


Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: Perl , Alison 
Sent: Thursday, February 21, 2019 10:46 AM
To: 'Clare Thornton'; 'Kelli Goodkowsky'
Cc: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] New York Qualifications for Histology Supervisor

Very exciting to hear NSH is working on it with CLIA! I hope the NYS 
legislature/OOP will take cues from them

And I just emailed my rep :)

Alison Perl, HTL(ASCP)CM
Anatomic Pathology Manager
(914) 302-8424
ap...@cmmedical.com


-Original Message-
From: Clare Thornton [mailto:cthorn...@dahlchase.com]
Sent: Thursday, February 21, 2019 12:39 PM
To: 'Kelli Goodkowsky'; Perl , Alison
Cc: 'histonet@lists.utsouthwestern.edu'
Subject: [EXTERNAL] RE: [Histonet] New York Qualifications for Histology 
Supervisor

NSH is currently working directly with CLIA to addresss issues such as this.  
Stay tuned!


Clare J. Thornton, HTL(ASCP)CM, QIHCCM
Lead Immunohistochemistry Tech
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthorn...@dahlchase.com




-Original Message-
From: Kelli Goodkowsky via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, February 21, 2019 12:11 PM
To: Perl , Alison 
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] New York Qualifications for Histology Supervisor

This is good information, Alison.  Thank you for sharing it.  Our Histology 
Program is in CT and I have many students from the southern part of the state, 
making NY licensing a viable option for them. It would be interesting to note 
how NY is defining “judgment” when it comes to the role of 
histotechnicians/histotechnologists.  I sit on the Educator’s Task force for 
the NSH and will bring this to their attention as well.


Kelli Goodkowsky, M.Ed., HT (ASCP)
Program Director, Histologic Science
President, Faculty Senate
Goodwin College
(860) 727-6917
kgoodkow...@goodwin.edu<mailto:kgoodkow...@goodwin.edu>
http://www.goodwin.edu










On Feb 21, 2019, at 11:51 AM, Perl , Alison via Histonet 
mailto:histonet@lists.utsouthwestern.edu>> 
wrote:

Hi Melissa
This is true, and a source of my neverending frustration with NYS Office of 
Professions. Stephanie Shulman 
(stephanie.shul...@health.ny.gov<mailto:stephanie.shul...@health.ny.gov>) is a 
good person at NYS to get clarification, but she will reiterate the same.

NYS has explained to me that "histotechs don't exercise judgment" and thus are 
not qualified to take on the role of supervisor, even for a histology-only 
laboratory. The supervisors here in my lab and myself all have Clinical Lab 
Technologist licenses, from when they were grandfathered back in 2007. I have 
techs who went to school specifically for Histology, but can never become 
supervisors. I don't know what anyone will do for the next generation of 
supervisors - your situation is a real fear.

Anyone in NYS who would like to raise this issue with your representatives, I 
encourage you to do so!!

Alison Perl, HTL(ASCP)CM
Anatomic Pathology Manager
CareMount Medical
(914) 302-8424
ap...@cmmedical.com<mailto:ap...@cmmedical.com>


-Original Message-
From: Melissa Owens via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, February 21, 2019 11:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] [Histonet] New York Qualifications for Histology Supervisor

Hello,

I have a question about the requirements to be a supervisor in New York. New 
York dept. of Ed states qualifications for a Medical Technologist/Laboratory 
Supervisor and a Cytotechnologist/Cytology Supervisor but no where does it 
mention qualifications for a Histototech/Histology Supervisor. So therefore, I 
have heard that in New York, a Histology Supervisor must qualify under the 
Medical Technologist/Laboratory Supervisor qualifications statues. This seems 
impossible to me as this would have to be an individual who becomes a Me

Re: [Histonet] Bone PMMA sections

[Patsy Ruegg via Histonet]

Stefano, after cutting the sections, dry them on a heat plate, then they can be 
stored dried stacked next to each other.


Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: MANTERO Stefano RIC 
Sent: Thursday, February 21, 2019 7:08 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bone PMMA sections

Good morning Histonetters!

I'm starting to cut out of bone samples included in PMMA.
I would like some suggestion how to storage the slides cut.

Many thanks in advance

Stefano

Dott. Stefano Mantero

Human Genome Laboratory  CNR-IRGB
c/o Humanitas Research Hospital

Via Rita Levi Montalcini (Ex Via Dainese)
20090 Pieve Emanuele (MI)

Ph. +39 02 8224 5164 (desk)
Ph. +39 02 8224 5177 (lab)
Fax +39 02 8224 5191



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legittimato è avvertito che trattenerlo, copiarlo, divulgarlo, distribuirlo a 
persone diverse dal destinatario è severamente proibito, ed è pregato di 
rinviarlo immediatamente al mittente distruggendone l'originale.
Grazie
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Re: [Histonet] Re guinea pig IHC

[Patsy Ruegg via Histonet]

good advise Carl and you asked some of the things I was wondering.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: Hobbs, Carl 
Sent: Saturday, February 2, 2019 12:38 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re guinea pig IHC



Ms Ruegg, as usual , gives excellent advice: avoid HRP.
Use Alk phos?
Block end. alk phos by using levamisole.

However, Ms Shivers does not state the fixation status of her FS.
Is the Gpig tissue perfused-fixed then frozen orfrozen as unfixed...then 
fixed?
Alsowhy 0.3% H2O2?
Use 3%kill the enzyme, not feed it?
NOT aq for FS
Make up in IMS ( 74OP)
No tissue disruption
However: you state that you get loadsa bubbles...so what? Is your section still 
attached to your slide?
Can you then carry out successful IF/IHC?
If yesno problem.
Sure, there's the argument that using a coagulant ( alcohol) in block is a No No
I never had a problemprovided that  the Formalin fixation was sufficient 
for unfixed crosections ( 15 mins)
I do STILL severely dislike FS ( sure, I spent many years in Diagnostic 
Histopath doing Operative FS)
 Why not use Pwax sections, Ms Shivers?

Curious-illy

Carl



Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge
Kings College London
London
SE1 1UL

020 7848 6813



From: histonet-requ...@lists.utsouthwestern.edu 

Sent: 02 February 2019 18:00
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 183, Issue 2


Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
 
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Today's Topics:

   1. Re: cytology listserv (Webster, Thomas S.)
   2. Re: guinea pig IHC (Patsy Ruegg)


--

Message: 1
Date: Fri, 1 Feb 2019 18:28:30 +
From: "Webster, Thomas S." 
To: "'histonet@lists.utsouthwestern.edu'"

Subject: Re: [Histonet] cytology listserv
Message-ID: <93fc6a1cc62f41f5ad5890786ab04...@crh.org>
Content-Type: text/plain; charset="us-ascii"

Haven't seen any cytology listservs except the one for members of the ASC.  
There are some cytology facebook pages where you could get questions answered. 
This Histonet listserv is very informative.


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Columbus Regional Hospital
2400 East 17th Street
Columbus, Indiana 47201

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Message: 2
Date: Fri, 1 Feb 2019 18:39:54 +
From: Patsy Ruegg 
To: Jan Shivers , histonet

Subject: Re: [Histonet] guinea pig IHC
Message-ID:



Content-Type: text/plain; charset="iso-8859-1"

Especially a very blood tissue like GP spleen.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: Patsy Ruegg 
Sent: Thursday, January 31, 2019 11:51 AM
To: Jan Shivers; histonet
Subject: Re: [Histonet] guinea pig IHC

In my experience it is not that GP have a higher peroxidase level, it is frozen 
sections in general that cannot be blocked with h202, unless they are fixed for 
a long time in formalin.  What are others experiences with h202 blocking on 
frozen sections.  I always  used an IHC detection system that did not require 
h202 blocking for frozen sections.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: Jan Shivers 
Sent: Tuesday, January 29, 2019 12:58 PM
To: histonet
Subject: [Histonet] guinea pig IHC

Has anyone ever performed IHC on frozen sections of guinea pig tissue?  I
am experiencing an enormous amount of bubblin

Re: [Histonet] guinea pig IHC

[Patsy Ruegg via Histonet]

Especially a very blood tissue like GP spleen.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: Patsy Ruegg 
Sent: Thursday, January 31, 2019 11:51 AM
To: Jan Shivers; histonet
Subject: Re: [Histonet] guinea pig IHC

In my experience it is not that GP have a higher peroxidase level, it is frozen 
sections in general that cannot be blocked with h202, unless they are fixed for 
a long time in formalin.  What are others experiences with h202 blocking on 
frozen sections.  I always used an IHC detection system that did not require 
h202 blocking for frozen sections.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: Jan Shivers 
Sent: Tuesday, January 29, 2019 12:58 PM
To: histonet
Subject: [Histonet] guinea pig IHC

Has anyone ever performed IHC on frozen sections of guinea pig tissue?  I
am experiencing an enormous amount of bubbling when doing the peroxidase
blocking step, even though I'm only using a 0.3% concentration of H2O2.
And when I say 'enormous', I mean it's like continuous champagne bubbles
rising out of the tissue, even after 20 minutes in the H2O2 solution.

I can't find anything in the literature that mentions guinea pigs having a
higher peroxidase content in their tissues.

Thanks for any help that anyone can provide.

Jan Shivers
Senior Scientist
IHC/Histology Section Manager
Pathology Teaching Program
Veterinary Diagnostic Laboratory
University of Minnesota
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive...@umn.edu

*Confidentiality Notice: This message, together with any attachments, is
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think you have received this message in error, please advise the sender and
then delete this message and any attachments immediately.*


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Re: [Histonet] guinea pig IHC

[Patsy Ruegg via Histonet]

In my experience it is not that GP have a higher peroxidase level, it is frozen 
sections in general that cannot be blocked with h202, unless they are fixed for 
a long time in formalin.  What are others experiences with h202 blocking on 
frozen sections.  I always used an IHC detection system that did not require 
h202 blocking for frozen sections.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: Jan Shivers 
Sent: Tuesday, January 29, 2019 12:58 PM
To: histonet
Subject: [Histonet] guinea pig IHC

Has anyone ever performed IHC on frozen sections of guinea pig tissue?  I
am experiencing an enormous amount of bubbling when doing the peroxidase
blocking step, even though I'm only using a 0.3% concentration of H2O2.
And when I say 'enormous', I mean it's like continuous champagne bubbles
rising out of the tissue, even after 20 minutes in the H2O2 solution.

I can't find anything in the literature that mentions guinea pigs having a
higher peroxidase content in their tissues.

Thanks for any help that anyone can provide.

Jan Shivers
Senior Scientist
IHC/Histology Section Manager
Pathology Teaching Program
Veterinary Diagnostic Laboratory
University of Minnesota
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive...@umn.edu

*Confidentiality Notice: This message, together with any attachments, is
intended only for the use of the individual or entity to which it is
addressed and may contain confidential or privileged information. If you
think you have received this message in error, please advise the sender and
then delete this message and any attachments immediately.*

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Re: [Histonet] IDH1 source

[Patsy Ruegg via Histonet]

Hi Richard,
How are you?  Dianova is a really good ab supplier, I used their CD31 for 
animal research and it was excellent.  Happy Holidays.  Patsy

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: John Garratt 
Sent: Sunday, December 16, 2018 2:37 PM
To: Anne van Binsbergen via Histonet
Subject: Re: [Histonet] IDH1 source

If you check out the assessments page at www.ciqc.ca<http://www.ciqc.ca> you 
will see EQA runs for IDH1. The protocols in the run reports will show the 
source of the Ab.

John

Sent from ProtonMail Mobile

On Sat, Dec 15, 2018 at 5:15 PM, Anne van Binsbergen via Histonet 
 wrote:

> Good morning to all
> Richard Cartun: we use IDH1 on FFPE tissue.
> We purchase from Dianova, Germany. (I believe they are still the only 
> suppliers worldwide).
> Purchased direct via their website.
> Vendors will also supply from the same source but at a higher price.
> Good luck
> Merry Christmas everyone!
> Anne
>
> Anne S van Binsbergen
> Supervisor, Histology Laboratory
> Pathology and Laboratory Medicine
> Sheikh Khalifa Medical City
> Abu Dhabi
> United Arab Emirates
>
> Sent from my iPhone
>
>> On Dec 15, 2018, at 10:00 PM, histonet-requ...@lists.utsouthwestern.edu 
>> wrote:
>>
>> Send Histonet mailing list submissions to
>> histonet@lists.utsouthwestern.edu
>>
>> To subscribe or unsubscribe via the World Wide Web, visit
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> or, via email, send a message with subject or body 'help' to
>> histonet-requ...@lists.utsouthwestern.edu
>>
>> You can reach the person managing the list at
>> histonet-ow...@lists.utsouthwestern.edu
>>
>> When replying, please edit your Subject line so it is more specific
>> than "Re: Contents of Histonet digest..."
>>
>>
>> Today's Topics:
>>
>> 1. IDH1 (Cartun, Richard)
>>
>>
>> --
>>
>> Message: 1
>> Date: Fri, 14 Dec 2018 20:38:38 +
>> From: "Cartun, Richard" 
>> To: "histonet@lists.utsouthwestern.edu"
>> 
>> Subject: [Histonet] IDH1
>> Message-ID:
>> <9215bd4b0ba1b44d962a71c758b68d2eac0e2...@hhcexchmb03.hhcsystem.org>
>> Content-Type: text/plain; charset="us-ascii"
>>
>> For those labs that are performing IDH1 IHC testing on FFPE tissue, which 
>> clone are you using and where do you get it? Thank you.
>>
>> Happy Holidays to everyone.
>>
>> Richard
>>
>> Richard W. Cartun, MS, PhD
>> Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
>> Proteomics Laboratory
>> Director, Biospecimen Collection Programs
>> Assistant Director, Anatomic Pathology
>> Hartford Hospital
>> 80 Seymour Street
>> Hartford, CT 06102
>> (860) 972-1596 (Office)
>> (860) 545-2204 (Fax)
>> richard.car...@hhchealth.org<mailto:richard.car...@hhchealth.org>
>>
>>
>> This e-mail message, including any attachments, is for the sole use of the 
>> intended recipient(s) and may contain confidential and privileged 
>> information. Any unauthorized review, use, disclosure, or distribution is 
>> prohibited. If you are not the intended recipient, or an employee or agent 
>> responsible for delivering the message to the intended recipient, please 
>> contact the sender by reply e-mail and destroy all copies of the original 
>> message, including any attachments.
>>
>>
>> --
>>
>> Subject: Digest Footer
>>
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>>
>> End of Histonet Digest, Vol 181, Issue 5
>> 
>
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[Histonet] Fw: Announcing the 12th annual retreat of applied immunohistochemistry and molecular pathology at the beautiful Marriott, Key Largo, FL

[Patsy Ruegg via Histonet]

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com




From: ancillaryp...@mac.com 
Sent: Saturday, October 7, 2017 6:03 PM
To: ihcrg ihcrg
Subject: Announcing the 12th annual retreat of applied immunohistochemistry and 
molecular pathology at the beautiful Marriott, Key Largo, FL

Dear colleagues at IHC Resource Group,

Following pre-hurricane preparation and 2 weeks of lack of electricity 
post-hurricane, we’re finally able to get our lives back on track, hence this 
delayed announcement for the 12th annual AIMP retreat. Please forward this 
email to as many folks as you can. Those of you who attended previous retreats 
will receive a decent discount as specified on the website.

Please let me know if you have any questions. I hope to see you in the FL Keyes 
in February.

Best regards,

Hadi

Hadi Yaziji, MD, FCAP, FASCP
Medical Director
Vitro Molecular Laboratories
8700 W. Flagler Street, Suite 100  l   Miami, FL 33174
www.vitromolecular.com<http://www.vitromolecular.com>
Vitro Molecular Laboratories | Anatomic 
Pathology<http://www.vitromolecular.com/>
www.vitromolecular.com
Vitro Molecular Laboratories provides routine and specialty anatomic pathology 
services to a loyal physician clientele.


hyaz...@vitromolecular.com<mailto:hyaz...@vitromolecular.com>
Office 305-267-7979  l   Fax 786-513-0175


[https://gallery.mailchimp.com/b4d3e9ff192b16fd68e3363a0/images/9e4a48fa-48d5-43fe-ac8c-f45b596f7767.jpg]




12th Annual International Retreat on Applied Immunohistochemistry & Molecular 
Pathology


Previous AIMP retreat attendees have attested that AIMP has been “the best 
pathology CME event ever attended”. Our 12th annual retreat will have the 
following list of speakers (arranged alphabetically):



Noah Brown, MD; University of Michigan

Richard Cartun, PhD; Hartford Hospital

Carol Cheung, MD, PhD., JD, FRCPC, University of Toronto

Brenda Cox, FHFMA, CPC, MT (ASCP)

Richard Eisen, MD; Banner Health

Joel Greenson, MD; University of Michigan

Jason Hornick, MD, PhD; Harvard University

Jeffrey Myers, MD; University of Michigan

Emina Terlacovich, MD, PhD; University of Saskatchewan

Megan Troxell, MD; Stanford University

Hadi Yaziji, MD, Vitro Molecular Laboratories



Location: Key Largo Marriott (Key Largo, FL, USA)

Date: February 4 - February 8, 2018


Topics at a glance:



Sunday, February 4

Welcome and Opening Remarks [Hadi Yaziji]

Pre-Test [Hadi Yaziji]

Interpreting FISH Assays by The Practicing Pathologist - General Principles  
[Hadi Yaziji]

Best of USCAP 2017: IHC Applications [Richard Eisen]

Developing Fit-For-Purpose IHC Assays [Carol Cheung]

Validation vs. Verification of IHC Assays:  The Clue is in the Test Performance 
Characteristics [Emina Torlakovic]

Tissue Tools are Power Tools for the IHC Laboratory.  [Carol Cheung]

Controlling the Controls in IHC – a Path to Standardization [Emina Torlakovic]

Monday, February 5:

Malignant Mesothelioma and Other Diffuse Pleural Tumors [Jeffrey Myers]

Role of the Pathologist in Diagnosis and Management of Patients with NSCLC 
[Jeffrey Myers]

Interpreting FISH Assays: Common Assays & Specific Scenarios  [Hadi Yaziji]

Molecular Genetics of Colon Cancer, what a practicing surgical pathologist 
needs to know [Joel Greenson]

Specimen Considerations for Solid Tumor Molecular Testing [Noah Brown]

PD-L1: Interpretation Pitfalls & Update on Utility as a Companion Diagnostic 
Assay [Hadi Yaziji]

Tuesday, February 6:

HER2 Testing: 2018 ASCO/CAP Guidelines - What’s New and Why [Hadi Yaziji]

Troubleshooting Diagnostic Immunohistochemistry [Rich Cartun]

Open Mic: Bring Your Own Questions - Part I  [Hadi Yaziji]

Best “Specialized” Markers (Myoepithelial, Cytokeratins, Neuroendocrine, 
Endothelial, Histocytic, Megakaryocitic, Erythroid, ETC) in IHC [Hadi Yaziji]

Surgical Pathology Case Presentations - Session 1 [Faculty]

Surgical Pathology Case Presentations - Session 2 [Faculty]

Wednesday February 7:

The Evolution of IHC for Soft Tissue Tumors in the 21st Century: From 
Differentiation to Molecular Genetics [Hornick]

Immunohistochemistry in Kidney Tumors: The New WHO Classification and Beyond 
[Megan Troxell]

Beyond Lineage: Diagnostic and Predictive Molecular IHC for Surgical 
Pathologists [Jason Hornick]

Immunohistochemistry in Transplant Pathology [Megan Troxell]

Surgical Pathology Case Presentations - Session 3 [Faculty]

Surgical Pathology Case Presentations - Session 4 [Faculty]



Thursday, February 8:

Essential Coding & Compliance: 2018 Update - Part I [Brenda Cox]

Essential Coding & Compliance: 2018 Update - Part II [Brenda Cox]

Open Mic: Bring Your Own Questions - Part II  [Hadi Yaziji]

Open Mic: Bring Your Own Questions - Part III  [Hadi Yaziji]

Surgical Pathology Case Presentations - Session 5 [Faculty]

Surgical Pathology Case Presentations - Session 6 [Fac

Re: [Histonet] Survey!!!!!!

[Patsy Ruegg via Histonet]

I have seen so many more problems with auto coverslippers, they break down, the 
cover does not hold up over time for archiving, etc., that I would would 
probably chose an autostainer.



Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com




From: Ifeoluwa Ajayi 
Sent: Saturday, April 1, 2017 6:11 AM
To: Patti Nelson - PNP Lab Consultant
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Survey!!

Personally I will prefer auto coverslipper, because cover slipping is
cumbersome and takes time.

Ajayi Ifeoluwa, BMLS, AMLSCN, MSc,
UCH Ibadan, Nigeria.

On Mar 31, 2017 8:00 PM, "Patti Nelson - PNP Lab Consultant via Histonet" <
histonet@lists.utsouthwestern.edu> wrote:

>
> Hi Everyone,
>
> I just wanted to get everyone's opinion. If you had to chose between
> buying a Auto Side Stainer or Auto Slide Cover Slipper, which one would you
> chose? Lets say your volume was around 60 to 80 blocks a day and you worked
> for a GI Lab. Everyone's input would be greatly appreciated.
>
>
>
> Sincerely,
>
> PATTI NELSON  H.T.(ASCP)
> PNP LABORATORY CONSULTANTS
> SUPERVISOR DGC/ZADEH LABS
> PO BOX 412
> CABAZON, CA. 92230
> 909-841-9761
> nelsonr...@verizon.net
> CONFIDENTIALITY NOTICE:This message and any included attachments are from
> Patti Nelson, PNP Laboratory Consultants and are intended only for the
> addressee. The information contained in this message is confidential and
> may contain privileged, confidential, proprietary and/or exemption from
> disclosure under applicable law.  Unauthorized forwarding, printing,
> copying, distribution, or use of such information is strictly prohibited
> and may be unlawful.  If you are not the addressee, please promptly delete
> this message and notify the sender ofthe delivery error by e-mail or you
> may call  909-841-9761.
>
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Histonet -- For the exchange of information pertaining to histotechnology and 
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RE: [Histonet] marking small samples

[Patsy Ruegg]

Thanks for your research Tim, this is good to know.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



> From: timothy.mor...@ucsf.edu
> To: mbu...@atlanticurologyclinics.com
> Date: Wed, 14 Jan 2015 23:28:49 +
> Subject: RE: [Histonet] marking small samples
> CC: histonet@lists.utsouthwestern.edu
> 
> Last year I found a paper to the effect that eosin may interfere with PCR 
> analysis. Our molecular group did an internal study and did not find any 
> significant issues.
> 
> But I just found another paper stating no interference:
> 
> http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3383644/
> 
> Am J Clin Pathol. Jul 2012; 138(1): 122-129.
> No Evidence for Interference of Hematoxylin and Eosin (HE) Staining in DNA 
> Testing: Utility of DNA Extraction from HE-Stained Archival Tissue Sections
> Teppei Morikawa, MD
> 
> 
> 
> im Morken
> Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
> UC San Francisco Medical Center
> San Francisco, CA
> 
> CONFIDENTIALITY NOTICE: This email message, including any attachments, is for 
> the sole use of the intended recipient(s) and may contain confidential, 
> proprietary, and/or privileged information protected by law. If you are not 
> the intended recipient, you may not use, copy, or distribute this email 
> message or its attachments. If you believe you have received this email 
> message in error, please contact the sender by reply email and destroy all 
> copies of the original message.
> 
> 
> -Original Message-
> From: Melissa Burns [mailto:mbu...@atlanticurologyclinics.com] 
> Sent: Wednesday, January 14, 2015 2:57 PM
> To: Morken, Timothy
> Cc: Yves Heremans; histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] marking small samples
> 
> I've been told Eosin affects a lot of molecular testing. We used to use it in 
> our process but discontinued to not interfere with future testing.  
> 
> Sent from my iPhone
> 
> > On Jan 14, 2015, at 5:31 PM, "Morken, Timothy"  
> > wrote:
> > 
> > We use eosin the processor alcohol. Also we use blue-tinted paraffin 
> > which helps with contrast on small bx
> > 
> > Tim Morken
> > Supervisor, Histology, Electron Microscopy and Neuromuscular Special 
> > Studies UC San Francisco Medical Center San Francisco, CA
> > 
> > CONFIDENTIALITY NOTICE: This email message, including any attachments, is 
> > for the sole use of the intended recipient(s) and may contain confidential, 
> > proprietary, and/or privileged information protected by law. If you are not 
> > the intended recipient, you may not use, copy, or distribute this email 
> > message or its attachments. If you believe you have received this email 
> > message in error, please contact the sender by reply email and destroy all 
> > copies of the original message.
> > 
> > 
> > -Original Message-
> > From: histonet-boun...@lists.utsouthwestern.edu 
> > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yves 
> > Heremans
> > Sent: Wednesday, January 14, 2015 2:03 PM
> > To: histonet@lists.utsouthwestern.edu
> > Subject: [Histonet] marking small samples
> > 
> > Dear Histonetters,
> > 
> > Does anyone know of a good method to mark (stain) small samples (tissue or 
> > cells) prior to paraffin embedding to aid in finding back more easily the 
> > sample in the paraffin block ?
> > Preferably something that would not interfere with subsequent antibody 
> > staining.
> > 
> > Yves
> > 
> > 
> > ___
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> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > 
> > ___
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RE: [Histonet] marking small samples

[Patsy Ruegg]

Eosin can be a problem in IHC as well especially if you use alk phos red 
detection.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



> From: mbu...@atlanticurologyclinics.com
> To: timothy.mor...@ucsf.edu
> Date: Wed, 14 Jan 2015 22:56:51 +
> Subject: Re: [Histonet] marking small samples
> CC: histonet@lists.utsouthwestern.edu; yves.herem...@vub.ac.be
> 
> I've been told Eosin affects a lot of molecular testing. We used to use it in 
> our process but discontinued to not interfere with future testing.  
> 
> Sent from my iPhone
> 
> > On Jan 14, 2015, at 5:31 PM, "Morken, Timothy"  
> > wrote:
> > 
> > We use eosin the processor alcohol. Also we use blue-tinted paraffin which 
> > helps with contrast on small bx
> > 
> > Tim Morken
> > Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
> > UC San Francisco Medical Center
> > San Francisco, CA
> > 
> > CONFIDENTIALITY NOTICE: This email message, including any attachments, is 
> > for the sole use of the intended recipient(s) and may contain confidential, 
> > proprietary, and/or privileged information protected by law. If you are not 
> > the intended recipient, you may not use, copy, or distribute this email 
> > message or its attachments. If you believe you have received this email 
> > message in error, please contact the sender by reply email and destroy all 
> > copies of the original message.
> > 
> > 
> > -Original Message-
> > From: histonet-boun...@lists.utsouthwestern.edu 
> > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yves 
> > Heremans
> > Sent: Wednesday, January 14, 2015 2:03 PM
> > To: histonet@lists.utsouthwestern.edu
> > Subject: [Histonet] marking small samples
> > 
> > Dear Histonetters,
> > 
> > Does anyone know of a good method to mark (stain) small samples (tissue or 
> > cells) prior to paraffin embedding to aid in finding back more easily the 
> > sample in the paraffin block ?
> > Preferably something that would not interfere with subsequent antibody 
> > staining.
> > 
> > Yves
> > 
> > 
> > ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > 
> > ___
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> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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RE: [Histonet] Decal acquired by StatLab

[Patsy Ruegg]

that is very interesting.  I always used Decal Chemical products really liked 
their formic acid Immunocal reagent.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



> Date: Thu, 8 Jan 2015 15:44:26 -0500
> From: rsrichm...@gmail.com
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Decal acquired by StatLab
> 
> I just got an e-mail noting that
> 
> "StatLab Medical Products, the leading manufacturer, developer and
> distributor of cost-effective histology, cytology and immunohistochemistry
> consumable supplies, is pleased to announce the acquisition of Decal
> Chemical Corporation of Tallman, NY."
> 
> Good time to be reminded that Decal™ is a trademarked name, and is not the
> generic term for any bottle of decalcifier on the shelf.
> 
> Decal was the most commonly used proprietary decalcifier when I started out
> in pathology fifty years ago. In a day when most labs prepared most of
> their own reagents, they always bought proprietary decalcifiers.
> 
> Nowadays people buy all solutions. I think the Herrn Inschpektors get you
> if you home-brew.
> 
> Bob Richmond
> Samurai Pathologist
> Maryville TN
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RE: And other crazy stuff. RE: [Histonet] cutting honey bees

[Patsy Ruegg]

we once played an april's fool joke on our Heme Pathologist.  We took the wings 
off a fly and embedded it in plastic (GMA) sectioned and stained it. Grossly It 
looked similar to the bone marrow core biopsies we did.  Heme pathologist are 
notorious for sticking slides under the microscope on high power without 
looking at them grossly.  We said we needed him to consult on this really 
strange bone marrow core because we couldn't figure out what the patient had.  
Most of our patients at the time were patients with leukemia or lymphoma.  Sure 
enough he stuck the slide under high power and probably even under oil 
immersion.  Trying to look at the morphology of individual cells.  He was 
stumped and was going to show it to another colleague, so we had to confess 
that it was a fly and this was a AF joke.  We thought he would have thought we 
were clever and laugh about it but he did not think it was funny.  We never did 
anything like that again.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



> Subject: RE: And other crazy stuff.  RE: [Histonet] cutting honey bees
> Date: Thu, 8 Jan 2015 15:27:58 -0500
> From: dhew...@hvhs.org
> To: mjo...@metropath.com; timothy.mor...@ucsf.edu; prueg...@hotmail.com; 
> r...@psu.edu; classic...@gmail.com; histonet@lists.utsouthwestern.edu
> 
> I have done a stink bug, spider and a few other creepy crawlers for my
> kids to look at under the scope, they have no idea what they are looking
> at but still love it.
> 
> Daniel Hewitt
> Histology Supervisor, HVS
> 412-749-7371
> 
> This email, including any attachments, is for the sole use of the
> intended recipient(s) and may contain confidential and privileged
> information.  Any unauthorized review, use, disclosure or distribution
> is prohibited.  If you are not the intended recipient, or an agent
> responsible for delivering the message to the intended recipient, please
> contact the sender by reply e-mail and delete and destroy all copies of
> the original message, including attachments.
> 
> Please note that any views or opinions presented in this e-mail are
> solely those of the author and do not necessarily represent those of
> Heritage Valley Health System.  The integrity and security of this
> message cannot be guaranteed on the internet.
> 
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael
> Ann Jones
> Sent: Thursday, January 08, 2015 3:20 PM
> To: Morken, Timothy; Patsy Ruegg; Roberta Horner; Douglas Gregg;
> 'histonet@lists.utsouthwestern.edu'
> Subject: Re: And other crazy stuff. RE: [Histonet] cutting honey bees
> 
> We did a goldfish once, interesting microscopically and difficult for
> peeling (lots of keratin?)
> Michael Ann Jones, HT (ASCP)
> Histology Manager
> Metropath
> 7444 W. Alaska Dr. #250
> Lakewood, CO 80226
> 303.634.2511
> mjo...@metropath.com
> 
> 
> 
> 
> On 1/6/15, 12:23 PM, "Morken, Timothy"  wrote:
> 
> >You crazy research people...OK, so what is the craziest thing you ever
> >had to cut, or were asked to cut?
> >
> >For me, not too bad, but embedding for EM and sectioning a single
> oocyte
> >that was nearly microscopic. I'll just say it took a LOT of thick
> >sections too face down to it without actually cutting through it.
> >
> >
> >Open the floodgates
> >
> >Tim Morken
> >
> >-Original Message-
> >From: histonet-boun...@lists.utsouthwestern.edu
> >[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy
> >Ruegg
> >Sent: Tuesday, January 06, 2015 11:13 AM
> >To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu
> >Subject: RE: [Histonet] cutting honey bees
> >
> >for the whole bee I probably would process and embed it in glycol
> >methacrylate (gma) it is much harder and would give better sections, we
> >have done zebra fish and several other harder tissues including
> calcified
> >bone in GMA.
> >
> >Cheers,
> >Patsy
> >
> >Patsy Ruegg, HT(ASCP)QIHC
> >Ruegg IHC Consulting
> >40864 E Arkansas Ave
> >Bennett, CO 80102
> >H 303-644-4538
> >C 720-281-5406
> >prueg...@hotmail.com
> >
> >
> >
> >> From: r...@psu.edu
> >> To: classic...@gmail.com; histonet@lists.utsouthwestern.edu
> >> Date: Sat, 3 Jan 2015 23:15:33 +
> >> Subject: RE: [Histonet] cutting honey bees
> >> CC: 
> >> 
> >> I sectioned and stained honey bee and yellow jacket stingers years
> ago.
> >> 

RE: [Histonet] Anyone using Biogenex stainers

[Patsy Ruegg]

I was curious about that too.  Don't they have that large fancy machine they 
claim does ISH and everything?


Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



> From: timothy.mor...@ucsf.edu
> To: histonet@lists.utsouthwestern.edu
> Date: Thu, 8 Jan 2015 18:43:41 +
> Subject: [Histonet] Anyone using Biogenex stainers
> 
> Does anyone use a Biogenex stainer these days? They used to be a premier 
> company but seem to have disappeared over the last decade.  They still 
> display at NSH and have ads in various places but am not aware of any lab 
> that uses one.
> 
> Tim Morken
> Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
> UC San Francisco Medical Center
> Box 1656
> 505 Parnassus Ave
> San Francisco, CA 94143
> USA
> 
> 415.514-6042  (office)
> tim.mor...@ucsfmedctr.org<mailto:tim.mor...@ucsfmedctr.org>
> 
> CONFIDENTIALITY NOTICE: This email message, including any attachments, is for 
> the sole use of the intended recipient(s) and may contain confidential, 
> proprietary, and/or privileged information protected by law. If you are not 
> the intended recipient, you may not use, copy, or distribute this email 
> message or its attachments. If you believe you have received this email 
> message in error, please contact the sender by reply email and destroy all 
> copies of the original message.
> 
> 
> 
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RE: And other crazy stuff. RE: [Histonet] cutting honey bees

[Patsy Ruegg]

The coolest thing I cut was 600 yo deer bone.  One of our pathologist did 
archeology as a hobby and wanted me to section it, it was petrified as hard as 
a rock.  We tried everything to soften it to no avail.  We ended up cutting 
with a diamond wire lapidary saw without embedding it in anything.  Could make 
sections as thin as 30 microns.  Had a heck of a time trying to get sections to 
adhere to a glass slide and the sections would not take any stain.  We did end 
up looking at it with a fluorescent scope.  We could see rings like a tree of 
bone turn over.  This is how tetracycline labeling of bone first came about.  
Someone way back stuck a piece of dinosaur bone under UV light and saw rings, 
apparently the animals eat moldy grain ( tet is made from mold) and it deposits 
where ever new bone is being laid down, it also happens to fluoresce.  I spent 
25 years in a metabolic bone disease lab, we treated the patient with a course 
of tetracycline then waited for a period of time, I think it was 10-14 days, 
then the patient took another dose of tet and then within a day or 3 we 
biopsied their bone usually from the illiac  crest fixed it in methanol because 
the tetracycline was water soluble then processed it into GMA plastic without 
decal.  Unstained 5 micron sections cut with a tungsten carbide blade were 
reviewed with a fluorescent scope revealing the two labels of tet, since we 
knew the time between doses we could measure the area between the two labels 
and report them out as bone growth in mm per day.  People with severe lack of 
bone turn over would just have one single label meaning they were not making 
much bone.  

Cheers,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



> From: r...@psu.edu
> To: timothy.mor...@ucsf.edu; prueg...@hotmail.com; classic...@gmail.com; 
> histonet@lists.utsouthwestern.edu
> Subject: RE: And other crazy stuff.  RE: [Histonet] cutting honey bees
> Date: Tue, 6 Jan 2015 20:54:10 +
> 
> The oddest things I cut were the honey bee and yellow jacket stingers.  I've 
> done plant stamen, reptiles, fish and I believe another insect.  I usually 
> tell the students that are working on a research project to give me a sample 
> they don't care about so I can see if I can do what they want.
> 
> But I had oddities that I didn't have to section like during hunting season a 
> hunter killed a deer and there was a mass on the trachea that he wanted 
> tested to make sure the deer was okay to eat.  I got the sample and when I 
> tried to gross it I found a very hard shiny silver object.  I told the 
> pathologist whose case it was that the mass was from a bullet did he still 
> want histo done. No.
> 
> The other interesting one was the egg shell.
> The conversation went something like this.
> Pathologist:  Can you section this egg shell
> Me: No it's too hard.
> P: Can't you decal it
> M: That's not going to work.
> P: Did you try.
> M: No
> P: Don't you think you should try first.
> M: Okay fine but it is no going to work.
> 
> Put a piece of eggshell (made of calcium) into some decal solution (that 
> removes calcium) and watch the egg shell bubble and disappear.  I did get to 
> tell the pathologist "I told you so"
> 
> Roberta Horner
> Penn State University
> Animal Diagnostic Lab
> 
> -Original Message-
> From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu] 
> Sent: Tuesday, January 06, 2015 2:24 PM
> To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet@Lists. Edu
> Subject: And other crazy stuff. RE: [Histonet] cutting honey bees
> 
> You crazy research people...OK, so what is the craziest thing you ever had to 
> cut, or were asked to cut?
> 
> For me, not too bad, but embedding for EM and sectioning a single oocyte that 
> was nearly microscopic. I'll just say it took a LOT of thick sections too 
> face down to it without actually cutting through it.
> 
> 
> Open the floodgates
> 
> Tim Morken
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
> Sent: Tuesday, January 06, 2015 11:13 AM
> To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu
> Subject: RE: [Histonet] cutting honey bees
> 
> for the whole bee I probably would process and embed it in glycol 
> methacrylate (gma) it is much harder and would give better sections, we have 
> done zebra fish and several other harder tissues including calcified bone in 
> GMA.
> 
> Cheers,
> Patsy
> 
> Patsy Ruegg, HT(ASCP)QIHC
> Ruegg IHC Consulting
> 40864 E Arkansas Ave
> Bennett, CO 80102
> H 303-644-4538
> C 720-

RE: [Histonet] cutting honey bees

[Patsy Ruegg]

for the whole bee I probably would process and embed it in glycol methacrylate 
(gma) it is much harder and would give better sections, we have done zebra fish 
and several other harder tissues including calcified bone in GMA.

Cheers,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



> From: r...@psu.edu
> To: classic...@gmail.com; histonet@lists.utsouthwestern.edu
> Date: Sat, 3 Jan 2015 23:15:33 +
> Subject: RE: [Histonet] cutting honey bees
> CC: 
> 
> I sectioned and stained honey bee and yellow jacket stingers years ago.  They 
> wanted to show the difference between the stingers.  I wasn't sure what to do 
> so I processed and handled like everything else.  I was able to get some good 
> sections.  I put 6 stingers in each block and cut several sections figuring 
> there should be at least one good stinger in each block and it worked.
> Roberta Horner
> Penn State University
> Animal Diagnostic Lab
> 
> From: histonet-boun...@lists.utsouthwestern.edu 
> [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg 
> [classic...@gmail.com]
> Sent: Saturday, January 03, 2015 6:08 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] cutting honey bees
> 
> Has anyone had experience embedding and cutting honey bees. I am sure
> there are some issues with the harder exoskeleton. Would that have to
> be dissected away first. I am considering helping a student with a
> science fair project on bees.
> 
> Douglas Gregg
> Veterianary pathologist
> 
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RE: [Histonet] HELP! Need some old fashioned histology advice

[Patsy Ruegg]

I have done something similar to this but I used tissue that was fixed but not 
processed and embedded, this is called enblock labeling, I infiltrated the 
fixed tissue with the IHC reagents, in a vial/tube, the blocking reagents, then 
the antibody, then the detection reagents and DAB, then dehydrated the tissue.  
I used vials or tubes on a platform shaker and would infiltrate reagents for 
days, then after it was done I infiltrated and embedded the tissue in glycol 
methacrylate (GMA) so that I could section it, it actually worked.  The tissue 
was already IHc LABeled so all I did to the 5 micron sections after they were 
cut was a hematoxylin counterstain, this was mineralized bone so I had to 
embedd in something hard like GMA to section.

Will you remove the Celloidin before trying to do the IHC staining?  100 micron 
sections might be easy to float/handle using a glass pipette for transferring.  
Sounds like an interesting project, good luck and feel free to ask for advise 
and keep us posted on your progress.

Cheers,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



> Date: Sun, 4 Jan 2015 11:58:38 -0600
> From: jaylundg...@gmail.com
> To: mbmph...@gmail.com
> Subject: Re: [Histonet] HELP! Need some old fashioned histology advice
> CC: histonet@lists.utsouthwestern.edu; mary.me...@ucsf.edu
> 
> I can help with the old fashioned advice:
> 
> 
>- 1 scant teaspoon simple syrup
>- 2 dashes Angostura Bitters, plus more to taste
>- 1 half dollar–sized slice orange peel, including pith
>- 2 ounces good-quality rye or bourbon
>- 1 maraschino cherry
> 
>  As for the Histology, is there any reason you cannot mount the
> sections onto glass slides?  When I was working at Genentech they were
> cutting frozen sections through whole rabbits and mounting the sections on
> (giant) glass slides.  I think that rolling the tissue up, inserting it,
> and then removing it from a glass tube would destroy the tissue.
> 
>   Sincerely,
> 
> Jay A. Lundgren, M.S., HTL
> (ASCP)
> 
> On Sat, Jan 3, 2015 at 8:01 PM, Maria Mejia  wrote:
> 
> > First, the very best of holidays to everyone.
> >
> > Now for the histology part.   Our lab's focus is on the early stages of
> > Alzheimer's Disease in the Brainstem
> > using celloidin processing & embedding for IHC staining.  This year, our
> > lab will be receiving 6 post-mortem
> > whole human brains (1 every other month).  After fixation, processing &
> > celloidin embedding, the whole brain
> > will be serially cut at 100um thick.  Each brain section will be 5 inches
> > x 4.5 inches in size.
> >
> > I will given 250 of these whole brain sections to stain for tau
> > IHC...that's 1500 whole brain sections/year!!!
> > 1) Does anyone have experience doing manual IHC staining of large
> > free-floating brain sections?
> > 2) What type of staining tools, dishes or other essential equipment can
> > anyone recommend?
> > 3) What's the most efficient way to stain 250 sections for batch IHC
> > staining - such as transferring batch
> > sections (maybe 5-10) from reagent to reagent?
> > 4) What type of batch apparatus to use?
> >
> > As for the antibody & ABC steps, I was thinking of placing each section
> > inside a large glass cigar tube
> > (yep, people use large glass tubes with fitted cap to store cigars), with
> > 5ml of antibody or ABC reagent & gently agitate on
> > a shaker/rotator at room temp during the incubation.  Does anyone have
> > ideas on this?
> >
> > Please, any ideas, suggestions or recommendation anyone can provide will
> > be most greatly appreciated.
> >
> > Best regards
> > Maria Mejia
> > UCSF
> > Department of Neurology
> > San Francisco, CA
> >
> >
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> >
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RE: [Histonet] (no subject)

[Patsy Ruegg]

I would think you would have to validate IHC for MOHS the same as FFPE, once 
the validation process is done maybe controls would not be necessary.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net


> From: chapm...@health.missouri.edu
> To: histonet@lists.utsouthwestern.edu
> Date: Tue, 2 Dec 2014 21:22:38 +
> Subject: [Histonet] (no subject)
> 
> Does anyone know if you have to use controls for IHC on MOHS frozen section 
> procedures to satisfy CAP requirements?
> 
> Thank you,
> 
> Cherie Chapman, BS, HT, HTL (ASCP)
> Associate Director of Dermatopathology Laboratory
> University of Missouri Department of Dermatology
> University Physicians Medical Building
> Phone: (573) 884-0123
> Fax: (573) 884-0834
> 
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RE: [Histonet] Bone Marrow processing using Immunocal

[Patsy Ruegg]

I use a platform shaker and slosh the sample around while it is in decal 
solution at RT, just make sure it is well fixed before you decal, Immunocal is 
5% formic acid decal solution it is not a fixative and it is a bit slower than 
some of the rapid decal reagents out there but is much better for IHC.  When I 
was in HemePath we would fix for about 6 hrs before decaling on the shaker for 
2-4hrs depending on the size (thickness) of the bone bx.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net


> Date: Mon, 17 Nov 2014 14:05:28 -0500
> From: carolyn.barn...@va.gov
> To: Histonet@lists.utsouthwestern.edu
> CC: 
> Subject: [Histonet] Bone Marrow processing using Immunocal
> 
> I am looking for a Bone Marrow procedure that uses Immunocal as the
> decalcifier. I am particularly interested in how long they fix in
> Immunocal and if heat is used in any way . Our lab  just started using
> this product because we are under the impression that it gave better ISH
> results.
> 
> Thank-you all so much for any assistance. I really appreciate everyone's
> help.
> 
>  
> 
> Carolyn K. Barnes
> 
> Histology Supervisor
> 
> McGuire VA Medical Center
> 
> Richmond, VA 23249
> 
> Ph: 804-675-5000, x2158
> 
>  
> 
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RE: [Histonet] Decalcified Bone Clearing time

[Patsy Ruegg]

Make sure that you fix well in formalin before you decal (what are you using?), 
then I rinse off the decal solution (I use formic acid) and put them back into 
formalin before putting on the tissue processor for a longer than usual 
schedule and especially give them longer time to infiltrate in paraffin, at 
least 3 changes for 2 hours each after dehydration and clearing in xylene.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net


> From: wa...@livemail.uthscsa.edu
> To: histonet@lists.utsouthwestern.edu
> Date: Mon, 17 Nov 2014 19:07:42 +
> Subject: [Histonet] Decalcified Bone Clearing time
> 
> Hey everyone! I'm having some issues about clearing the bone tissues after 
> they get done dehydrating from decalcification.  I know that over clearing 
> the bone tissues in Xylene for too long can cause them to become very brittle 
> and I'm afraid that that was what was happening.  The bone blocks are not 
> very bigtheir weights are listed as 0.222g, 0.100g, and 0.052g.  The 
> biggest bone block is about 4mmx4mmx4mm so it's not very big I would say.  I 
> cleared with 50% Ethanol/50% Xylene for 1 hour, and then I changed it with 
> 100% Xylene for another hour.  The bone blocks become clear as expected but 
> they begin to get a yellowish-tan tinge to them.  I'm not the most 
> experienced but from what I've studied, I don't think that 2 hours in Xylene 
> is overkill by any means so maybe it's something that I'm doing 
> subconsciously.if anyone has any suggestions are ideas please let me 
> know! :)
> 
> 
> Trevor Jordan Wait
> University of Texas Health Science Center, San Antonio
> Class of 2017 MD Candidate
> Abilene Christian University Class of 2013 Graduate
> B.S.  Biochemistry
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RE: [Histonet] TRAP staining protocol troubleshooting

[Patsy Ruegg]

Actually trap will work if you pre incubate it in 0.2M Tris buffer Ph9 (use 
sodium hydroxide to pH) for 1 hour at 37dc then proceed with the trap stain 
without rinsing.  Check the stain in 20 min as it develops quickly after this.  
This protocol was given to me by the late great Hermina and it works really 
well even on formic acid decaled FFPE bone in my hands.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net


From: prueg...@hotmail.com
To: wa...@livemail.uthscsa.edu
Subject: RE: [Histonet] TRAP staining protocol troubleshooting
Date: Mon, 17 Nov 2014 13:22:24 -0700




Trap dose not work on decalcified bone unless you have decaled with EDTA in my 
experience.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net


> From: wa...@livemail.uthscsa.edu
> To: histonet@lists.utsouthwestern.edu
> Date: Mon, 17 Nov 2014 18:58:50 +
> Subject: [Histonet] TRAP staining protocol troubleshooting
> 
> Hello histonetter heroes!  I have an issue with a TRAP staining protocol that 
> I'm currently using that involves Fast Red Violet LB Salt for the staining of 
> the Osteoclast and then Fast Green as a counterstain.  The osteoclast are 
> suppose to stain Red Violet and the background is suppose to stain green.  
> However, I'm not seeing any Red Violet Osteoclast but just really really 
> Green tissue.  The bone I was using was cortical bone but surely there would 
> at least 1 Osteoclast in all of sections that I had.  Here is the protocol 
> that I'm using:
> 
> 
> Trap Staining solution Mix: Trap Basic Incubation medium, Fast Red Violet LB 
> Salt, and Naphthol AS-MX Phophaget substrate mix
> 
> 
> Procedure:
> 
> 1. Place TRAP staining solution mix in staining dish and pre-warm to 37 
> degree celsius in waterbath
> 
> 2. Deparafinnize slides and rehydrate through graded ethanols to distilled 
> water.
> 
> 3. Place slides in pre-warmed TRAP staining solution mix and incubate at 37 
> celsius for 30 mins or until control is developed.
> 
> 4. Rinse in distilled wtaer
> 
> 5. Counterstain with 0.02% Fast Green for 30 seconds and rinse quickly in 
> distilled water.
> 
> 6. Dehydrate quickly thorugh graded alcohols, 5 seconds each, clear in Xylene 
> and mount.
> 
> 
> The problem is that the main staining with the Fast Red is not very 
> substantial.at least not as much as it should be.  When I take the slide 
> from the water bath, the tissue section is only slightly red...and may even 
> look more orangish yellowwhich is a long ways away from red violet.  Then 
> when I counterstain with Fast Green, I feel that the Fast Green stain is so 
> prominent that it just superimposes the Fast Red stain.  For anyone that has 
> experience with TRAP staining or Fast Red Violet staining then that would be 
> awesome to hear your input!
> 
> 
> 
> Trevor Jordan Wait
> University of Texas Health Science Center, San Antonio
> Class of 2017 MD Candidate
> Abilene Christian University Class of 2013 Graduate
> B.S.  Biochemistry
> ___
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[Histonet] TC nuclear stain

[Patsy Ruegg]

TC experts.  One of my former students is doing a TC stain and the nuclear 
stain with iron hematoxylin is washing out.  I remember that I had this problem 
myself with weigerts/iron heme, I always got a weaker purple stain rather than 
black like it was supposed to be.  Do you think it would help to repeat the 
nuclear stain at the end of the stain as well as in the beginning, make fresh 
hematoxylin, etc

Thank you for your advise,

Patsy

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net
  
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Re: [Histonet] AFB stained smears

[Patsy Ruegg]

U r right ffpe control would not be ideal but unless u have access to a source 
+ for tb u could make a bunch of smears to keep for controls that may be your 
only option

Sent from my iPhone

> On Nov 7, 2014, at 10:38 AM, "Glenn Hauck" 
>  wrote:
> 
> The odd time I receive a request to do a Zeihl Neelsen stain for acid fast 
> bacteria on a smear for Micro. My question is what kind of control should I 
> be using? I have tissue processed with AFB, but I feel this should not be 
> used because the smear is not processed the same way as my AFB control block.
> 
> Thanks
> 
> Glenn Hauck
> Charge Technologist
> Pathology
> Queen Elizabeth II Hospital
> Grande Prairie, AB T8V 2E8
> 
> 780-538-7429 Work Main
> 780-538-7184 Work Office
> glenn.ha...@albertahealthservices.ca
> 
> 
> 
> This message and any attached documents are only for the use of the intended 
> recipient(s), are confidential and may contain privileged information. Any 
> unauthorized review, use, retransmission, or other disclosure is strictly 
> prohibited. If you have received this message in error, please notify the 
> sender immediately, and then delete the original message. Thank you.
> ___
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RE: [Histonet] Permanent red, Fuchsin and Vector Red not exactly red...

[Patsy Ruegg]

agree Biocares Warp Red is very strong but still has to avoid alcohol.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net


From: l...@premierlab.com
To: yves.herem...@vub.ac.be; histonet@lists.utsouthwestern.edu
Date: Thu, 6 Nov 2014 13:43:09 -0700
Subject: RE: [Histonet] Permanent red, Fuchsin and Vector Red not exactly   
red...
CC: 

Yves
 
Try Biocares Warp Red, you will still need to air dry the slides prior to 
coverslipping do not place them in alcohol, air dry, place in xylene and then 
mount.
 
Liz
 
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com
 
March 10, 2014 is Histotechnology Professionals Day
 
Ship to Address:
 
Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
 
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yves Heremans
Sent: Thursday, November 06, 2014 1:12 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Permanent red, Fuchsin and Vector Red not exactly red...
 
Dear Histonetters,
 
I am trying to combine a peroxidase-DAB staining (brown) with an alkaline 
phosphatase-staining for detection of two proteins that do not overlap.
I have already tried Dako’s Permanent Red and Fuchsin+ and Vector’s Vector Red 
as AP substrates but I am not getting a clear red color, rather something 
brownish which is not very different in color from the DAB reaction product.
Is there anything I can do to obtain a clearer red color ?
 
Yves
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Re: [Histonet] Tissue falling off positive slides

[Patsy Ruegg]

Make sure yr sections r airdryed completely before u bake thm 

Sent from my iPhone

> On Nov 6, 2014, at 10:34 AM, "Pardue, Judith"  
> wrote:
> 
> WE are having trouble with tissue coming off our h&e slides. Our heater is 
> set on 65 degrees for 10 minutes. Any suggestions. We use Fisher blue 
> positive slides.
> 
> Judith Pardue
> CHI Memorial
> Chatt. Tn. 37343
> judith_par...@memorial.org
> 
> This electronic mail and any attached documents are intended solely for the 
> named addressee(s) and contain confidential information. If you are not an 
> addressee, or responsible for delivering this email to an addressee, you have 
> received this email in error and are notified that reading, copying, or 
> disclosing this email is prohibited. If you received this email in error, 
> immediately reply to the sender and delete the message completely from your 
> computer system.
> ___
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RE: [Histonet] Ebola

[Patsy Ruegg]

We tried very hard not to do CJD cases if we could help it, as I recall any 
suspect cases were sent to Case Western Reserve, i worked with someone there 
who developed a protocol using strong formic acid, it was amazing how good the 
tissue preservation was using formic acid as a fixative.  I heard about 
possible cases in our histo dept at the u where they ended up throwing away 
microtomes and other instruments who might have come in contact with CJD 
tissue, crazy, wasn't my department so I was not up on all the details.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net


From: jwat...@gnf.org
To: histonet@lists.utsouthwestern.edu; sfin...@providencehealth.bc.ca
Date: Thu, 9 Oct 2014 21:31:38 +
Subject: RE: [Histonet] Ebola
CC: 

Correction,  due to years of formalin abuse,  it was 1 hour in concentrated 
formic acid.  We then processed and cut them with all other cases.  A few cases 
slipped through with just formalin fixation and additional precautions needed 
to be taken.  That said the easiest  and primary route of infection for CJD is 
ingestion.  Needless to say we did not eat any of the tissue.
 
 
A simple and effective method for inactivating virus infectivity in 
formalin‐fixed tissue samples from patients with Creutzfeldt‐Jakob disease 
 
Paul Brown, MD, 
 Axel Wolff, DVM and 
 D. Carleton Gajdusek, MD
 
-Show Affiliations
 
Laboratory of CNS Studies, National Institute of Neurological Disorders and 
Stroke, National Institutes of Health, Bethesda, MD. 
 
 
 
doi: 10.1212/WNL.40.6.887  Neurology June 1990   vol. 40  no. 6  887  
 
 
 
Abstract
Full Text (PDF)
 
 
Abstract 
 
We fixed brains from hamsters infected with scrapie virus in (1) formalin, (2) 
phenol-saturated formalin, (3) formalin with a 1-hour immersion in formic acid, 
or (4) phenol-saturated formalin with a 1-hour immersion in formic acid. In 
addition, we used the formalin-formic acid procedure on brains from mice 
infected with the virus of Creutzfeldt-Jakob disease. Formic acid proved 
superior to phenol in respect to both disinfection and tissue preservation, 
almost completely eliminating virus infectivity in sections that were 
histologically indistinguishable from formalin-fixed material. The inclusion of 
a formic acid step in routine formaldehyde tissue fixation will thus provide 
histologic sections of excellent quality, and virtually eliminate the risk of 
handling infectious material in the subsequent neuropathologic processing of 
tissues from patients with CJD. 
© 1990 by the American Academy of Neurology
 
 
James Watson HT  ASCP
GNF  Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel858-332-4647
Fax   858-812-1915
jwat...@gnf.org
 
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson
Sent: Thursday, October 09, 2014 1:57 PM
To: 'Emily Brown'
Cc: Histonet@Lists. Edu
Subject: RE: [Histonet] Ebola
 
Back in the 80 NIH did extensive studies on CJD,  their protocol requires 24 
hours in formalin, 24 hours in formic acid, followed by 48 hours in formalin, 
then paraffin processing.  Worked with CJD at Frederick Cancer Research in 
collaboration with the group from NIH.  If I find the publications I will 
forward them.
 
James Watson HT  ASCP
GNF  Genomics Institute of the Novartis Research Foundation Scientific 
Technical Leader II, Histology Tel858-332-4647 Fax   858-812-1915 
jwat...@gnf.org
 
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Brown
Sent: Thursday, October 09, 2014 1:40 PM
Cc: Histonet@Lists. Edu
Subject: Re: [Histonet] Ebola
 
If formalin didn't kill CJD, what did you use? Just curious.
 
Emily
 
"By bitching and bitching and bitching, they could exhaust the drama of their 
own horror stories. Grow bored. Only then could they accept a new story for 
their lives. Move forward."
 
-Chuck Palahniuk, "Haunted"
 
On Thu, Oct 9, 2014 at 4:34 PM, Patsy Ruegg  wrote:
 
> Well said Pam, it is just assumed that formalin will eliminate the 
> biohaz for Ebola, I doubt if that has been conclusively proven yet, 
> remember we only discovered fairly recently that formalin fixation did 
> not protect us from CJD
>
> Patsy Ruegg, HT(ASCP)QIHC
> Ruegg IHC Consulting
> 40864 E Arkansas Ave
> Bennett, CO 80102
> H 303-644-4538
> C 720-281-5406
> prueg...@hotmail.com
> pru...@ihctech.net
>
>
> > Date: Thu, 9 Oct 2014 20:26:16 +
> > From: mucra...@comcast.net
> > To: tanyaabb...@catholichealth.net
> > Subject: Re: [Histonet] Ebola
> > CC: histonet@lists.utsouthwestern.edu
> >
> > Take Brett's advise and use that a

RE: [Histonet] Ebola

[Patsy Ruegg]

Well said Pam, it is just assumed that formalin will eliminate the biohaz for 
Ebola, I doubt if that has been conclusively proven yet, remember we only 
discovered fairly recently that formalin fixation did not protect us from 
CJD

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net


> Date: Thu, 9 Oct 2014 20:26:16 +
> From: mucra...@comcast.net
> To: tanyaabb...@catholichealth.net
> Subject: Re: [Histonet] Ebola
> CC: histonet@lists.utsouthwestern.edu
> 
> Take Brett's advise and use that as guidleine.  We don't know as much as we 
> should about these viruses.   Pam 
> 
> - Original Message -
> 
> From: "Tanya Abbott"  
> To: "Histonet"  
> Sent: Thursday, October 9, 2014 2:03:47 PM 
> Subject: [Histonet] Ebola 
> 
> Dare I ask?! Are any Pathology labs discussing what to do with 
> specimens/precautions, etc. regarding a person with a potential Ebola 
> infection? 
> 
> Tanya G. Abbott RT (CSMLS) 
> Manager Technologist, Histology/Cytology 
> St. Joseph Medical Center 
> Reading, PA 19603-0316 
> ph  610-378-2635 
> fax 610-898-5871 
> email: tanyaabb...@catholichealth.net 
> 
> This electronic mail and any attached documents are intended solely for the 
> named addressee(s) and contain confidential information. If you are not an 
> addressee, or responsible for delivering this email to an addressee, you have 
> received this email in error and are notified that reading, copying, or 
> disclosing this email is prohibited. If you received this email in error, 
> immediately reply to the sender and delete the message completely from your 
> computer system. 
> ___ 
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
> 
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RE: [Histonet] RE: Ebola

[Patsy Ruegg]

All samples should be handled with universal precautions assuming they may be 
infectious, formalin fixation is considered an eliminator of biologic hazard 
for most things like Ebola, but then there is always CJD that it does not work 
for.  I would not be doing any frozen sections on samples with suspected 
biologic hazard since they do not get fixed in formalin typically, although you 
can fix in formalin then rinse it out and infiltrate with sucrose to make fixed 
frozen sections.  Keep calm and use your common sense and vast experiences.

Cheers,
Patsy 

Here is a story for you.  Back in the late 70's early 80's when we didn't know 
much about HIV or HPV I worked in a Heme/Path lab at the U.  One of the 
pathologist I worked with was studying genital wort's and lesions getting her 
samples from the local sexually transmitted disease clinic.  This was back in 
the day when T&B cells had to be done on cell suspensions, frozen sections or 
smears/touch preps not aldehyde fixed.  The antibodies and detection systems 
did not yet work in FFPE tissues.  I prepared unfixed frozen samples on a ton 
of these biopsies.  I double gloved, used UP and got thru this.  Looking back 
on that project I do not think I would have done it knowing what I know now.   
There is a reason or two that Histotechnology is the most dangerous job I guess.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net


From: jwat...@gnf.org
To: joyce.we...@emoryhealthcare.org; tanyaabb...@catholichealth.net; 
histonet@lists.utsouthwestern.edu
Date: Thu, 9 Oct 2014 19:52:12 +
CC: 
Subject: [Histonet] RE: Ebola

Have it delivered in formalin and let it fix completely.
 
 
 
"Formalin fixation eliminates the biologic hazard associated with transporting 
samples containing infectious EBO virus,  Thus, formalin 
fixation provides an adequate noninfectious specimen for EHF diagnosis, 
."
 
A quote from:
 
Long-Term Disease Surveillance in Bandundu Region, Democratic Republic of the 
Congo: A Model for Early Detection and Prevention of Ebola Hemorrhagic Fever 
 
Ethleen S. Lloyd, and  C. J. Peters, et al
 
 
+ Author Affiliations
 
Centers for Disease Control and Prevention, Atlanta, Georgia; World Health 
Organization and US Agency for International Development and Ministry of 
Health, Kinshasa, Hôpital Général de Référence de Kikwit, Kikwit, and Mosango 
Mission Hospital, Mosango, Democratic Republic of the Congo; Médecins sans 
Frontières—Belgium, Brussels, Belgium 
 
 
+ Author Notes
 
↵* Current affiliation: Institut de Médecine Tropicale, Antwerp, Belgium 
(M.A.B.); Médecins sans Frontières—Belgium, N'Djamena, Chad (E.V.); World 
Health Organization, Kinshasa, Democratic Republic of the Congo (J.K.). 
 
Reprints or correspondence: Dr. Pierre E. Rollin, Special Pathogens Branch, 
NCID/DVRD, Mailstop G-14, Centers for Disease Control and Prevention, 1600 
Clifton Road, Atlanta, GA 30333 (p...@cdc.gov).
 
 
James Watson HT  ASCP
GNF  Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel858-332-4647
Fax   858-812-1915
jwat...@gnf.org
 
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Thursday, October 09, 2014 12:22 PM
To: 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Ebola
 
Handle with universal precautions as always is our emphasis.
 
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
 
 
 
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
 
This e-mail, including any attachments is the property of Saint Joseph's 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya
Sent: Thursday, October 09, 2014 3:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ebola
 
Dare I ask?! Are any Pathology labs discussing what to do with 
specimens/precautions, etc. regarding a person with a potential Ebola infection?
 
Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 
19603-0316 ph  610-378-2635 fax 610-898-5871
email: tanyaabb...@catholichealth.net
 
This electronic mail and any attached documents are intended solely for the 
named addressee(s) and contain confidential informa

RE: [Histonet] Blades for cutting resin on a microtome

[Patsy Ruegg]

I used to have a triangle glass knife holder insert for my Leica microtome or I 
would use the tungsten carbide knives.  It depends on what you are cutting.  if 
it is calcified bone the glass knives scratch too much and they are only 1/2 
inch wide so you have to cut smaller soft tissues with them.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net


From: abri...@brightinstruments.com
Date: Fri, 12 Sep 2014 17:32:47 +0100
To: gkey...@uwhealth.org
Subject: Re: [Histonet] Blades for cutting resin on a microtome
CC: histonet@lists.utsouthwestern.edu; veronique.bar...@gmail.com; 
exp...@brightinstruments.com

Yes brightinstruments, com make glass knife holder and tungsten carbide tipped 
knives for microtomes,
KR,Alan Bright
 
Sent from my iPhone
 
> On 12 Sep 2014, at 15:49, "Keyser Gerald  T"  wrote:
> 
> I've only cut resin with a glass or diamond knife in an ultramicrotome. If 
> you are attempting to do it in a regular microtome, you would need a special 
> blade holder. I don't know if any microtome manufactures make glass knife 
> holders. 
> 
> You make the glass blades yourself using special glass. Here is a link to the 
> glass strips: 
> http://www.sigmaaldrich.com/catalog/product/sigma/g2528?lang=en®ion=US
> 
> Here is a cheap jig and diamond glass cutters it make the knifes:
> https://www.emsdiasum.com/microscopy/products/preparation/glassknife.aspx
> 
> I've never made glass knives by hand using a hand held diamond cutter and 
> jigs. I imagine that it would take practice.  
> 
> I've only used a specialized maker:
> https://www.emsdiasum.com/microscopy/products/histology/tissue_stainer.aspx
> 
> You paint a bit of nail polish underneath the glass edge and put a bit of 
> distilled water on the edge. You then section the block floating the sections 
> on the water. Use an eyelash manipulator to pick up the 5um thick sections 
> and place on a bubble of water on the slide. Evaporate the water droplet on 
> the slide. If you've done it right, the sections won't look like origami. If 
> it does, then practice until it doesn't. 
> 
> Gerry 
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Véronique 
> Barrès
> Sent: Friday, September 12, 2014 9:33 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Blades for cutting resin on a microtome
> 
> Happy Friday Histonetters!
> 
> I am working on a histology platform in a research center and someone came to 
> me last week and asked to cut blocs of resin (JB-4 resin) on the microtome. I 
> never cut anything else than paraffin, so I was wondering if some of you had 
> advices for me?
> 
> They never did it neither and took their protocol in a paper where it was 
> said that we should use disposable glass knife instead of standard metal 
> blades. Are any of you ever used those knife? Where do you buy them?
> We have an old Leica RM2125.
> 
> Thanks for your advices!
> 
> Véronique
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> 
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RE: [Histonet] HS Class Project

[Patsy Ruegg]

I forgot to mention these can be human tissues if they are already processed.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net


> From: prueg...@hotmail.com
> To: histonet@lists.utsouthwestern.edu
> Date: Tue, 9 Sep 2014 15:31:24 -0600
> Subject: [Histonet] HS Class Project
> 
> Colleagues,
> 
> Can
>  anyone help me with this request below, I am trying to do a simple IHC 
> project in the local HS Biotech class.  I have the reagents donated but I
>  need tissue.
> 
> I
>  am helping Overland HS do some histology in their Biotech class we want
>  to do a simple IHC project this year, is there any chance you could get
>  us 12 slides prepared from normal pancreas and 6 from abnormal/diabetic
>  pancreas, if you have blocks I could make slides and get them back to 
> you?
> 
> Best regards,
> Patsy
> 
> Patsy Ruegg, HT(ASCP)QIHC
> Ruegg IHC Consulting
> 40864 E Arkansas Ave
> Bennett, CO 80102
> H 303-644-4538
> C 720-281-5406
> prueg...@hotmail.com
> pru...@ihctech.net
> 
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[Histonet] HS Class Project

[Patsy Ruegg]

Colleagues,

Can
 anyone help me with this request below, I am trying to do a simple IHC 
project in the local HS Biotech class.  I have the reagents donated but I
 need tissue.

I
 am helping Overland HS do some histology in their Biotech class we want
 to do a simple IHC project this year, is there any chance you could get
 us 12 slides prepared from normal pancreas and 6 from abnormal/diabetic
 pancreas, if you have blocks I could make slides and get them back to 
you?

Best regards,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net
  
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RE: [Histonet] Re: Neutrophil staining

[Patsy Ruegg]

used to cae back in the day and it picked up granules perfectly and was very 
easy to do

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net


> Date: Sun, 7 Sep 2014 21:04:06 -0400
> From: rsrichm...@gmail.com
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Re: Neutrophil staining
> 
> Hans B Snyder at Histologistics in Worcester, Massachusetts asks:
> 
> >>I am looking to stain neutrophils using special stains and antibodies in
> mice tissue. I wanted your opinion about the best methods to do so. I am
> thinking of using the May-Grünewald Giemsa and T-blue for specials and
> myeloperoxidase and Ly6G for the antibodies.<<
> 
> I haven't done it and don't know if it works on mice, but histochemical
> staining for chloroacetate esterase is extremely simple to do, and supposed
> to be quite specific.
> 
> Bob Richmond
> Samurai Pathologist
> Maryville, Tennessee
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[Histonet] Microtome for HS Biotech Class

[Patsy Ruegg]

Dear Histo Peeps,

I am on the advisory board for a local high school Biotech class and have been 
helping them process, embed, cut, and stain tissue (they use chicken livers 
can't use human).  My husband fixed up an old black AO microtome for them a few 
years ago so they actually cut sections.  This year the teacher contacted me 
and told me that her usual class of about 20-24 has gone to 32 and she doesn't 
think she will have enough time to teach everyone to cut a section with just 
one microtome.  She asked me if I knew anyone locally they could borrow a 
microtome from, so I have been asking around but since I am retired I do not 
have access to many resources anymore.  I thought I would put this out to our 
histo community especially if there is someone local in Colorado that could 
help us out.  I also thought that one of the equipment vendors like Rankin 
might be able to help out.  This is a Cherry Creek High School (Overland) and 
the teacher said the school would give out receipts for tax purposes for any 
donations received.  Thank you all for considering this request.

Best regards,
Patsy

PS
Maybe our past and new Region VII directors (Janet and Jane) could help with 
this???

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net
  
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[Histonet] Consult for taking abs from RUO to IVD

[Patsy Ruegg]

Colleagues,

I am consulting with an antibody vendor who needs a consultant to help them 
take their abs from RUO to IVD.  If you know anyone who does this type of work 
you could recommend would you please have them contact me directly and I will 
pass their contact info on.

Best regards,

Patsy

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net
  
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RE: [Histonet] On the lighter side...

[Patsy Ruegg]

39 years for me

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net


> Date: Thu, 7 Aug 2014 11:59:44 -0700
> From: cont...@excaliburpathology.com
> To: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] On the lighter side...
> 
> 36 years.
> 
>  
> Paula K. Pierce, HTL(ASCP)HT
> President
> Excalibur Pathology, Inc.
> 5830 N Blue Lake Dr. Please note new address!
> Norman, OK 73069
> 405-759-3953 Lab
> 405-759-7513 Fax
> www.excaliburpathology.com
> 
> 
> 
>  From: "Sullivan, Beatrice" 
> To: 'Douglas Porter' ; 
> "histonet@lists.utsouthwestern.edu"  
> Sent: Thursday, August 7, 2014 1:51 PM
> Subject: RE: [Histonet] On the lighter side...
>  
> 
> 44 years here.
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter
> Sent: Thursday, August 07, 2014 2:39 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] On the lighter side...
> 
> How long have you been a registered histotech?  36 years here.  You???
> 
> 
> 
> Douglas A. Porter, HT (ASCP) 
> Grossing Technician 
> IT Coordinator
> 
> Cancer Registrar 
> 
> 
> CAP-Lab, PLC 
> 2508 South Cedar Street
> Lansing, MI 48910-3138 
> 
> 517-372-5520 (phone) 
> 517-372-5540 (fax) 
> 
> <mailto:doug.por...@caplab.org> doug.por...@caplab.org 
> 
> <http://www.caplab.org/> www.caplab.org  
> 
> 
> 
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> 
> This message, and any included attachments, are from Virtua Health or its 
> related affiliates and is intended only for the addressee(s). The information 
> contained herein is privileged, proprietary or may include confidential 
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> 
> 
> 
> 
> 
> 
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[Histonet] HS IHC project

[Patsy Ruegg]

Friends,

I am going to help a local HS class (a Biotech class) do
 a simple IHC project.  This class already processes tissue, embeds, 
sections and does an H&E stain.  My husband fixed up an old black AO
 microtome for them to use and they actually cut sections.  They cannot 
use human tissue there so what they have available is mouse spleen 
already ffpe.  I was thinking of using a rab antibody that works on ffpe
 ms spleen.  Ki67 comes to mind?  It has been a while since I was in the
 lab so I am consulting with you all to make sure a rab poly or rab mono
 Ki67 will indeed stain a ffpe ms spleen?  If you have evidence of this 
can you tell me which ab was used successfully.  If you can think of a 
better rab ab to use on the ffpe ms spleen for this project let me 
know.  It would help if we could keep it simple for antigen retrieval.

One of our kind vendors will be donating IHC reagents for this HS class project.

I hope the IHC meeting is going well in Vegas, wish I was there.

Cheers,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net
  
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[Histonet] FW: IHC training

[Patsy Ruegg]

From: pru...@ihctech.net
To: pru...@ihctech.net
Subject: FW: IHC training
Date: Fri, 23 May 2014 09:52:27 -0600

Patsy Ruegg prueg...@hotmail.com or pru...@ihctech.net




Can anyone help Mrs. Lawal coming from Nigeria to the NSH S/C in Austin in Aug 
this year?  She wants to visit a lab for some more IHC training.  We tried to 
find a lab in the Austin area but failed.  We were going to let her come to U 
of Colorado but the timing is not good for them.  I will post this to histonet 
and the NSH FB group site as well.  Mrs. Lawal's email addresses are listed 
below. 

ojades...@yahoo.com


Message from Eve Kemble:


This is Eve from the IHCRG. I am at Dartmouth Hitchcock Medical Center in NH, 
and I have a question for you: I met Mrs. Lawal in Vancouver  at the NSH S/C in 
2012. She was one of the keynote speakers at that meeting.Mrs. Lawal is in 
charge of a histology lab at the Lagos University Teaching Hospital in 
Nigeria.She currently uses a Ventana stainer  to do the IHC in her lab.Mrs.
 Lawal is planning to attend the S/C in Austin, and would like to stay 
on for a while after the S/C so that she could visit another lab for 
some further practical training and to observe how things are done 
elsewhere.Can you recommend any lab/s that would be willing to host Mrs. Lawal? 
Mrs. Lawal is very knowledgeable in IHC and is very keen in improving the 
processes that are done in her lab. Any information will be most welcome. Many 
thanks for your help! Cheers,  Eve Kemble.  
eve.kem...@gmail.com
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net











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RE: [Histonet] Freelance Histology

[Patsy Ruegg]

this may certainly not be on the up and up but I have heard about these kinds 
of operations for years, not sure how they can do it but I know of a lab that 
did clinical skin bx samples for years and years and I am sure they were not 
Clia/CAP/or even GLP, I guess since it is Histology all the responsibility lies 
with the Pathologist reading the slides, I never thought it was very kosher, at 
least for this one case it is not going on anymore, but it would not surprise 
me if there were Derm and GI labs all over not really accredited to be doing 
histology on human samples but they still do.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net


From: l...@premierlab.com
To: l...@premierlab.com; hrfulk...@gmail.com; histonet@lists.utsouthwestern.edu
Date: Tue, 22 Apr 2014 16:28:09 -0600
Subject: RE: [Histonet] Freelance Histology
CC: 

Please excuse my grammar, yikes.  
 
Liz
 
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com
 
March 10, 2014 is Histotechnology Professionals Day
 
Ship to Address:
 
Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
 
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth 
Chlipala
Sent: Tuesday, April 22, 2014 4:23 PM
To: H R; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Freelance Histology
 
Cutting is one thing such as like control slides, but staining that’s a whole 
different thing, you will have chemical waste that needs to be disposed of 
properly.  You will need appropriate ventilation, etc.   Correct me if I am 
wrong but I don’t think you could be performing any clinical work, it would 
have to be research.  Even though you might be only one person I still think 
you are responsible for all applicable OSHA guidelines.  
 
Just my two cents.
 
Liz
 
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com
 
March 10, 2014 is Histotechnology Professionals Day
 
Ship to Address:
 
Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
 
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of H R
Sent: Tuesday, April 22, 2014 4:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Freelance Histology
 
Has  anyone heard of people cutting and staining slides out of your garage or 
some kind of similar set up, like picking up overflow from different companies 
/labs? I overheard a conversation about someone doing this in their retirement. 
Labs send them the block and all they do is cut and do HE stain and return both 
and bill? What kinds of regulations would that entail? I don't know if that 
could be true. Maybe for research facilities?
 
--
*H*
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RE: [Histonet] Speaking of picrosirius red...

[Patsy Ruegg]

I have done picrosirius red for years and never used  phosphomolybdic acid so 
it would be hard to say what it's purpose is, mine works fine without it.

> Date: Tue, 12 Nov 2013 19:15:43 -0500
> From: amosbro...@gmail.com
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Speaking of picrosirius red...
> 
> Hi,
>  In light of the current discussion about picrosirius red I would like
> to revisit a question that was asked a while back. If it was answered
> publicly, I apologize I probably missed it and didn't see it in the
> archives. Someone asked what is the purpose of the phosphomolybdic acid
> step before the picrosirius red. If anyone has a good answer for this I
> would like to know. While it is mostly curiosity, I think knowing what each
> step in a stain is for helps keep the quality and consistency of stains.
> 
> Thanks,
> Amos
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RE: [Histonet] Mouse lung IHC false positive/background issue

[Patsy Ruegg]

if any part of your detection is something anti mouse you will get binding to 
all mouse tissue Ig not just the Ig associated with the antibody u are using.  
What is the host species of the antibody being used?  Most labeled polymer 
detection systems use a link step which is usually rab anti mouse for mouse 
antibodies to link the rab portion to the labeled polymer 3rd step which is 
usually goat anti rab if you are using a rab antibody on mouse tissue u can 
skip the link step and go directly to the goat anti rab labeled polymer, 
avoiding anti mouse reagents. 

From: nmargar...@luriechildrens.org
To: lu...@sbcglobal.net; histonet@lists.utsouthwestern.edu
Date: Fri, 11 Oct 2013 20:01:55 +
Subject: RE: [Histonet] Mouse lung IHC false positive/background issue
CC: rebel...@gmail.com

I have same problem for other Abs using in on mouse lung tissues... My 
secondary and tertiary have no problems on other tissues for negative controls 
as well
 
Naira
**
Confidentiality Note: This message (including any attachments) is intended for 
the use of the named recipient(s) only and may contain confidential and/or 
proprietary information. If you are not the intended recipient, please contact 
the sender and delete this message. Any unauthorized use of the information 
contained in this message is prohibited.
 
 
Ranked nationally in all 10 pediatric specialties by U.S. News & World Report  
(LCHOC Ver 1.0)
 
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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lu Ze
Sent: Friday, October 11, 2013 2:38 PM
To: histonet@lists.utsouthwestern.edu
Cc: rebel...@gmail.com
Subject: [Histonet] Mouse lung IHC false positive/background issue
 
Hello everyone,
 
I forwarded message from my colleague, Rebecca (see below) to see if any one 
with IHC experience on mouse lung tissue have suggestion.
 
 
===
 
I'm having trouble with my IHC. I am staining for human MTDH (specific for 
human) on mouse tumor and lung sections from my xenograft mouse model. All of 
my tumors stained positive, but not the lung sections (which could possibly be 
the results). My real problem is that my negative control normal lung section 
stained positive for MTDH. I saw positive staining on the tissue near the 
outside and in the bronchioles.
 
I know the problem is not the secondary antibody because I did 2 slides (normal 
lung and previously positive tumor) without primary ab and they showed no 
positive staining.
 
My antibody is a rabbit monoclonal antibody at 1:200 for 1 hour. I'm blocking 
with 1% BSA in TBST for 1 hour. I'm using DAB substrate kit for developing.
 
What would you recommend?
 
Thank you,
 
Ze
 

Ze Lu, Ph.D.
Optimum Therapeutics, LLC
9363 Towne Centre Dr., Suite 110
San Diego, CA 92121
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RE: [Histonet] Re: [IHCRG] p40

[Patsy Ruegg]

Richard now the ab companies are really going to hate you advocating diluting 
predilute's further isn't that a sacrilege, what are we going to do with you, 
no negs and now diluting predilutes.  I am just kidding I do it all the time 
myself. 
Cheers, Patsy

Date: Tue, 14 May 2013 13:33:21 -0400
From: rcar...@harthosp.org
To: ih...@googlegroups.com; histonet@lists.utsouthwestern.edu; 
jtay...@meriter.com
CC: 
Subject: [Histonet] Re: [IHCRG] p40

I have been very impressed with BioCare's "p40" monoclonal predilute. 
We are able to use it a 1:5 dilution on our Bond Max platform. 
Excellent immunoreactivity so far!
 
Richard
 
 
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax
 
 
>>> "Taylor, Jean"  5/10/2013 1:53 PM >>>
 
Hi everyone,
 
I’m looking into ordering the p40 antibody for one of our pathologists.
He wants to use it to help distinguish between adenocarcinoma and
squamous cell ca in lung. I’m wondering what labs are using, the
monoclonal or polyclonal antibody, and where you purchase it from. I
haven’t found that a lot of companies that carry it. Any info would be
helpful.
 
Thanks,
 
Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI
 
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RE: [Histonet] Happy 79th Birthday Jules Elias

[Patsy Ruegg]

Me too Tim when was that had to have been late 80'$ or so.  Jules was worried 
about med techs taking  over ihc from us so he came to the NSH bod and asked us 
to develop a certification in ihc for ht's since NSH is not a credentialing 
body Freida CArson suggested we petition ascp and that is how the QIHC exam 
came about.  Thank you Jules for all u have done look where we are today there 
is not an AP lab worth mentioning that doesn't do ihc and many are also doing 
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Re: [Histonet] P16 on Leica Bond

[Patsy Ruegg]

Really 1:4?___
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Re: [Histonet] Leica Bond III Opinions

[Patsy Ruegg]

Love the bond it does not break down very often for me and service is great i 
can run all the antibodies i want for me even research animal because i have a 
more open research unit cleaning the covertiles is a little tedious but we make 
it work and replace them with new ones often beats disposing of all the waste 
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[Histonet] FW: [IHCRG] ms abs on rabbit tissue

[Patsy Ruegg]

  _  

From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of
Patsy Ruegg
Sent: Tuesday, January 15, 2013 9:49 AM
To: ih...@googlegroups.com
Subject: [IHCRG] ms abs on rabbit tissue

 

Does anyone have a mouse ab worked out on rabbit tissue?  We got a ms ab
from Abcam that is supposed react in Human, rat and rabbit but we are having
trouble getting it to stain the rabbit tissue.  Please advise.

 

Patsy

 

Patsy Ruegg, HT(ASCP)QIHC

Ruegg IHC Consulting, LLC

40864 Arkansas Ave

Bennett, CO 80102

Fax: 720-859-4110

 <mailto:pru...@ihctech.net> pru...@ihctech.net

 

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RE: [Histonet] Medical Director credentials on reports

[Patsy Ruegg]

I have a question about this issue as well, does the medical director have
the be an M.D. or can they be a PhD?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histot...@imagesbyhopper.com
Sent: Thursday, January 10, 2013 6:46 AM
To: 'Histonet@Lists. Utsouthwestern. Edu'
Subject: [Histonet] Medical Director credentials on reports

Does anyone know, and maybe have the regs, whether either the Joint
Commission or the CAP *require* that the Medical Director's credentials be
included on the genlab and surgical reports?  I have heard that it *is* a
requirement and now am hearing that it is *not* one.  Clarification would be
nice!

Example:  

Joe Smith
Medical Director

OR

Joe Smith, M.D.
Medical Director

Thanks!
Michelle
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[Histonet] FW: Announcing the 7th international retreat of applied IHC and molecular pathology (AIMP)

[Patsy Ruegg]

 

 

  _  

From: Hadi Yaziji, MD [mailto:i...@pathlearning.com] 
Sent: Thursday, January 10, 2013 9:55 AM
To: Patsy Ruegg
Subject: Announcing the 7th international retreat of applied IHC and
molecular pathology (AIMP)

 

Dear colleagues,

 

It is our honor and pleasure to announce the 7th Annual International
Retreat on Applied Immunohistochemistry and Molecular Pathology (AIMP), from
February 3-7, 2013, in the sunny and beautiful Miami (Coral Gables), FL. The
reason we have been offering this retreat for six years (and hopefully many
more years to come) is because we maintained focus on the issues that matter
the most to the practicing pathologist. In the way of example , the 7th
annual retreat will continue to focus on the following aspects of IHC and
molecular applications in our practice:

1. Among the >2000 new antibodies, can you offer a short list of new
antibodies that I can benefit from their utility in surgical pathology?
2. What type of IHC platform should we bring to our pathology lab?
3. How can we effectively apply QA measures into our IHC lab and molecular
lab?
4. What kind of impact do the ASCO/CAP guidelines have on our gross room
workflow and documentation?
5. What do I need to do to ensure our lab is compliant with these
guidelines, and compliant with other CAP and CLIA requirements?
6. What kind of FISH or molecular assays could our lab benefit from bringing
in-house?
7. What are the most common molecular assays in oncologic pathology that I
should be familiar with?
8. How do I build a molecular lab in my hospital?
9. Can you show me real - life examples of the staining pattern of
antibodies I use in my practice?

10. What are the clinical applications of the molecular oncology assays, and
what is the biology behind the testing and treatment?

11. What's the best IHC panel to properly classify lung cancer?

12. What's the best IHC panel to properly work up undifferentiated malignant
neoplasm

13. What's the best IHC panel to properly work up metastatic carcinoma of
unknown primary site?

13. What's the best IHC panel for mesothelioma vs. carcinoma?

14. How do I workup germ cell tumors by IHC?

15. How do I do IHC on cytology preparations?

16. What are the cancer predictive/prognostic assays that are not based on
IHC, ISH or FISH?

17. What are the reimbursement issues facing payments for IHC, FISH and
molecular assays?


In essence, there is nothing that you deal with in your day-to-day surgical
pathology practice as far as ancillary testing is concerned that is not
going to be discussed at this retreat in some way, shape or form. We go
straight to the bottom line without presenting to you any non-evidence based
science that is not going to affect your practice of surgical pathology. 


We constructed the educational program to address the above practical
questions, as you can note from perusing the Schedule page. We assembled a
group of the most experienced colleagues in the field, including Rich
Cartun, Rich Eisen, David Hicks, Todd Barry, Jason Hornick, Savitri
Krishnamurthy, and myself. For the detailed schedule, please click here.
<http://pathlearning.dmanalytics2.com/click?u=http%3A%2F%2Fpathlearning.com%
2FPathology_Learning_Center%2FCourse_Schedule.html&i=1&d=32419694-Y16V-4V1V-
Z8VZ-86Y3Z2Y64ZZZ&e=pruegg%40ihctech.net&a=048187V2-0814-46Z2-8U36-Y621V17Z6
9Z3> 

On average, 40% of attendees are repeat attendees. Our colleagues who
attended the first five courses truly enjoyed this event. In fact, many of
them described it as "the most practical CME pathology course I've ever
attended". We hope you will enjoy the upcoming course. It is very exciting
and we look forward to seeing you there.

Please click here
<http://pathlearning.dmanalytics2.com/click?u=http%3A%2F%2Fwww.pathlearning.
com%2F&i=2&d=32419694-Y16V-4V1V-Z8VZ-86Y3Z2Y64ZZZ&e=pruegg%40ihctech.net&a=0
48187V2-0814-46Z2-8U36-Y621V17Z69Z3> Â for detailed information of the
program and how to register. This year, the ballroom can only accommodate a
maximum of 175 participants in classroom seating. We recommend that you
register fairly soon to guarantee a spot.

Best regards,

Hadi Yaziji, MD and Rich Eisen, MD
Course Directors

 

Unsubscribe
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RE: [Histonet] holly nuclei bat man

[Patsy Ruegg]

Yes this all makes good sense and explains why I may not be able to have
control of this because I do not always do the tissue fixation and
processing myself.  That first step of adequately fixing (cross linking the
proteins) to protect them from tissue processing and AR are so
important.  The one thing I can control is to make sure the slides are
completely dry before baking them as Renee mentioned.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 80102
Fax: 720-859-4110
pru...@ihctech.net
-Original Message-
From: Gudrun Lang [mailto:gu.l...@gmx.at] 
Sent: Sunday, January 06, 2013 4:32 AM
To: 'Patsy Ruegg'
Cc: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] holly nuclei bat man

I have a quiet semi-scientific explanation. Formalin-fixation stabilizes
proteins via crosslinking. AR "solves" methylol-adducts due to formaldehyde
and perhaps also some crosslinks. Nuclei also have a protein-scaffold,
filled with nucleinacids. It seems like a "ladder" or "hole in tights". It
seems like there is a kind of tension, after breaking a crosslink the hole
"blubbs" out.
This is also seen with pretreatment in hybridization-procedures. The more
enzymatic and heat-retrieval the more holes.  
And there is a direct relationsship between fixation-quality and
AR-strength.

Bye Gudrun

-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Patsy
Ruegg
Gesendet: Samstag, 05. Jänner 2013 21:38
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] holly nuclei bat man

Happy New Year everyone!

 

Can we start a discussion once again on what causes holes in nuclei?  It is
pretty clear in my experience it has something to do with heating at least
for me with antigen retrieval.  When I do HIER at ph 8 especially I am
seeing a lot of holes in nuclei.  H&E on same tissue alone without HIER/IHC
doesn't seem to exhibit this artifact (holes in the nuclei).  I bet it can
also happen if the tissue is not fixed well enough to protect it from
paraffin processing but right now I am seeing it more after HIER, don't see
it as much on my IHC slides with no AR or when I use eier instead of hier.
Also notice that ph6 HIER and Ph9 HIER are not as bad as when I use Ph8 for
the same times and temps. 

 

Cheers,

 

Patsy

 

Patsy Ruegg, HT(ASCP)QIHC

Ruegg IHC Consulting, LLC

40864 Arkansas Ave

Bennett, CO 80102

Fax: 720-859-4110

 <mailto:pru...@ihctech.net> pru...@ihctech.net

 

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[Histonet] holly nuclei bat man

[Patsy Ruegg]

Happy New Year everyone!

 

Can we start a discussion once again on what causes holes in nuclei?  It is
pretty clear in my experience it has something to do with heating at least
for me with antigen retrieval.  When I do HIER at ph 8 especially I am
seeing a lot of holes in nuclei.  H&E on same tissue alone without HIER/IHC
doesn't seem to exhibit this artifact (holes in the nuclei).  I bet it can
also happen if the tissue is not fixed well enough to protect it from
paraffin processing but right now I am seeing it more after HIER, don't see
it as much on my IHC slides with no AR or when I use eier instead of hier.
Also notice that ph6 HIER and Ph9 HIER are not as bad as when I use Ph8 for
the same times and temps. 

 

Cheers,

 

Patsy

 

Patsy Ruegg, HT(ASCP)QIHC

Ruegg IHC Consulting, LLC

40864 Arkansas Ave

Bennett, CO 80102

Fax: 720-859-4110

 <mailto:pru...@ihctech.net> pru...@ihctech.net

 

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[Histonet] refurbished histology equipment

[Patsy Ruegg]

Does anyone have a favorite vendor they would recommend to me?  I am mostly
looking for cryostats right now, but interested in other things, tissue
processors, IHC stainers, etc.

 

Patsy Ruegg, HT(ASCP)QIHC

Ruegg IHC Consulting, LLC

40864 Arkansas Ave

Bennett, CO 80102

Phone: 303-644-4538

Fax: 720-859-4110

 <mailto:pru...@ihctech.net> pru...@ihctech.net

 

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[Histonet] Leica Bond RX

[Patsy Ruegg]

Good Day everyone,

 

Is anyone using the new Leica Bond RX research IHC/ISH autostainer?  If you
are I would love to hear any feed back on it you may have.

 

Best regards,

 

Patsy

 

 

Patsy Ruegg, HT(ASCP)QIHC

Ruegg IHC Consulting, LLC

40864 Arkansas Ave

Bennett, CO 80102

Phone: 303-644-4538

Fax: 720-859-4110

 <mailto:pru...@ihctech.net> pru...@ihctech.net

 

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[Histonet] 1 gal paraffin dispenser for training in Cozumel Mexico

[Patsy Ruegg]

I am posting this for a colleague David Davis who is doing some training of
techs in Cozumel and is really in need of a 1 gal paraffin dispenser for
that.  I have a 5 gal dispenser to donate but he thinks that is too big.  If
anyone has a 1 gal paraffin dispenser they would be willing to donate to
this cause we could probably provide you with a fedex acct no for shipping
it to use.

 

Thank you for considering this request,

 

Best regards,

 

Patsy

 

Patsy Ruegg, HT(ASCP)QIHC

Ruegg IHC Consulting, LLC

40864 Arkansas Ave

Bennett, CO 80102

Phone: 303-644-4538

Fax: 720-859-4110

 <mailto:pru...@ihctech.net> pru...@ihctech.net

 

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RE: [Histonet] decal affecting IHC

[Patsy Ruegg]

I agree with Liz and use 5-10% formic acid for decal for IHC work, on the
other hand if you are trying to demonstrate iron deposits or some enzyme
histochemical stains such as TRAP or Alk.phos you may need to use EDTA.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax: 720-859-4110
pru...@ihctech.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth
Chlipala
Sent: Friday, November 09, 2012 11:00 AM
To: 'Rene J Buesa'; Diana McCaig; 'histonet@lists.utsouthwestern.edu'
Subject: RE: [Histonet] decal affecting IHC

Diana

I agree with Jack, formic acid is the way to go in my opinion, we use 10%
formic acid routinely but have pushed it up to 20% on occasion when time was
an issue.  HCL can work if its controlled really well but you don't have
much wiggle room, you can over decal quite quickly, formic acid is a bit
more forgiving.  As for Rene's comment we have seen the exact opposite with
respects to the EDTA decal part.  Granted we do not run a lot of IHC on EDTA
decaled samples but on several occasions and with several antibodies we were
able to obtain good IHC staining in formic acid decaled samples but those
antibodies did not work well in EDTA decaled samples.  These were not direct
comparisons of the decalcification method on the same study, but on samples
from different studies so other factors could have affected the results.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, November 09, 2012 10:47 AM
To: Diana McCaig; 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] decal affecting IHC

IHC reactions can be affected by decalcification especially if:
1- the tissue is not perfectly fixed before going into decalcification
2- decalcification is done at temperature above room temp.
3- decal solution is too acid, specially made with inorganic acids.
If at all possible decalcification should be done with a chelating agent,
like EDTA
René J.



From: Diana McCaig 
To: "'histonet@lists.utsouthwestern.edu'"

Sent: Friday, November 9, 2012 12:34 PM
Subject: [Histonet] decal affecting IHC

Does decalcifying tissue  (Calex II) affect the antibody reaction for IHC in
bony tissue?

Diana

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RE: [Histonet] Microtome knives

[Patsy Ruegg]

There are knife sharpening services Sturkey and Dorn and Hart are two that
come to mind.  You can also find refurbished disposable blade holders and
buy disposable blades, the blade holder you get will determine if you use
low profile or high profile blades.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax: 720-859-4110
pru...@ihctech.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Massimo
Sent: Sunday, November 11, 2012 2:45 AM
To: Jon Krupp; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome knives

I prefer to sharpen my microtome knives by myself by hand.
I have a vintage Cambridge Rocking Microtome and despite its age it works
very well.
Sharpening is a time consuming for the first time, it's depends on the
conditions of the blade edge.
Once you have a nice cutting profile its maintenance it's quite easy and it
takes a few minutes by
stroking the knife on a flat glass with oil and a bit of aluminium oxide
powder (3 -1 micron grits).
For me sharpening and honing of a microtome knife has became a secondary
"hobby".
A solid knife has the advantage, compared to a disposable blade, to be
liable to less vibrations.

Kind Regards,
Massimo Tosi


"A humble Chemical
Engineer who loves Histology"






 Da: Jon Krupp 
A: Histonet@lists.utsouthwestern.edu 
Inviato: Venerdì 9 Novembre 2012 19:49
Oggetto: [Histonet] Microtome knives
 
Greetings

I need some advice regarding microtome knives. I am not  histo tech, I did
all my sectioning in a plant research lab, but now I find myself needing to
learn more about histo type methods.

We have microtomes, AO 820's, and we have a bunch of donated knives. I need
advice about whether it would be better to find a knife sharpener and use
the microtome knives we have, or check into getting a disposable knife
holder. 

When I was sectioning, we just used a simple razor blade holder. Now I see
references to high profile and low profile blades and holders, and I don't
know the difference. 

Anyone willing to help me out?

Thanks

Jon

Jonathan Krupp
Delta College
5151 Pacific Ave.
Box 212
Stockton, CA  95207
209-954-5284
jkr...@deltacollege.edu

Find us on Facebook @
Electron Microscopy at SJ Delta College







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RE: [Histonet] Help! Liver mistakenly processed in paraffin (had tobe in OCT instead)!

[Patsy Ruegg]

Yea if you were looking for fat that will be lost with paraffin processing,
so the oil red o for fat will not work.  I would not see any point in trying
to depara and then snap freeze, but if I did want to do that since the
tissue is formalin fixed I would depara, fix some more in formalin and then
infiltrate the tissue in 30% sucrose overnight before snap freezing, if you
do not do that fix tissue will not section well frozen.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax: 720-859-4110
pru...@ihctech.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
MacDonald
Sent: Tuesday, November 13, 2012 10:15 AM
To: z o n k e d
Cc: histonet@lists.utsouthwestern.edu;
histonet-boun...@lists.utsouthwestern.edu
Subject: Re: [Histonet] Help! Liver mistakenly processed in paraffin (had
tobe in OCT instead)!

It depends on what you are using the oil red o for.  Lipofuscin and ceroid 
can be demonstrated with an oil red o stain after processing.
Jennifer MacDonald




From:   z o n k e d 
To: "histonet@lists.utsouthwestern.edu" 

Date:   11/13/2012 08:53 AM
Subject:[Histonet] Help! Liver mistakenly processed in paraffin 
(had to be  in OCT instead)!
Sent by:histonet-boun...@lists.utsouthwestern.edu



Hello Histonetters,

First time writer, long time reader. I'm a newbie tech in academia and I
was given a simple task which I think I pretty much screwed up.

I should have embedded half of a mouse liver in paraffin for microtome
sectioning while the other half should have been embedded in OCT for
cryosectioning (for oil red o). I made the mistake last night of placing
both liver halves into the tissue processor. The liver I intended for OCT
embedding is now hard as wax. Is there any way to deparaffinize processed
organs and may I embed them in OCT for proper cryosectioning? I imagine
that the liver would get dehydrated, I would get crappy sections, and Oil
Red O won't work.

Any suggestions are welcome.

Thank you so much,

Zoe W.


-- 
"It costs nothing to say something kind. Even less to shut up altogether."

--Nathan Fillion
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[Histonet] radiation damage to nerves

[Patsy Ruegg]

Does anyone have any ideas on how we might demonstrate radiation injury to
peripheral nerves near the prostate (in a canine model).  we would like to
evaluate demyelinating changes and it has been suggested we might do this by
embedding the tissue in GMA plastic and cutting 2 micron sections and doing
toludine blue stain?  We are trying to avoid osmium and EM for this.
Any ideas?

 

Regards,

 

Patsy

 

Patsy Ruegg, HT(ASCP)QIHC

Ruegg IHC Consulting, LLC

40864 Arkansas Ave

Bennett, CO 80102

Phone: 303-644-4538

Fax: 720-859-4110

 <mailto:pru...@ihctech.net> pru...@ihctech.net

 

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RE: [Histonet] Stain for osteoporotic bone in Spurr plastic

[Patsy Ruegg]

Allison,

I had a great von kossa protocol for this but it was on glycol methacrylate
embedded bone, here it is, it uses the fluorescence property of eosin which
will stain the osteoid with the calcified bone covered by the silver von
kossa.

GMA is not removed
Rinse 5 micron sections in dih20, use clean glassware
Make 2% silver nitrate, put on the slides and expose them to uv (a lamp or
even in the window will do, for about 20 min.
The calcified bone should turn black
Rinse well in dih20, you can fix the silver with sodium thiosulfate but do
it quickly as it does remove some silver, rinse well in dih20
Hematoxylin nuclear stain, we used Gills#3 for 5 min., wash in tap water,
blue in ammonia water, wash well in tap water then to dih20
Make a 0.2% solution of aqueous eosin y, stain slides in this for 20 min.,
rinse in dih20 and airdry, coverslip with permount.
You can measure the calcified bone and nuclei in the light microscope, then
use a fluorescent scope with a fitc filter to see the osteoid, it will be
green just on the surface of the calcified bone which is black from the
silver.  We got a total sample area measurement, calcified area as a
percentage of total area, and osteoid area as a percentage of total area.
We did other measurements as well such as number of osteoclasts (we used a
Acid phosphotase (TRAP) enzyme histochemical stain for this) and number of
osteoblast (can use alkaline phosphotase enzyme histochemical stain for
these or we just counted them from the VK slide with the heme nuclear stain
by morphology, they will be lining the surface of the bone, very flat and
run together.)

Hope this helps.  Goldner TC is also used to measure osteoid but this worked
best for us.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax: 720-859-4110
pru...@ihctech.net
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff
Sent: Thursday, November 08, 2012 4:52 PM
To: Malandra, Allison E; Histonet
Subject: RE: [Histonet] Stain for osteoporotic bone in Spurr plastic

Allison,
I am not exactly certain if this protocol will work for Spurr's embedded
specimens, but here is the protocol that I use for deplasticized sections of
methyl methacrylate embedded undemineralized bone (non-decalcified):
Xylenes - warmed @ 50 C for 30 min w/ dip & dunk @ 15 min interval
Xylenes - warmed @ 50 C for 30 min w/ dip & dunk @ 15 min interval
Xylenes - warmed @ 50 C for 30 min w/ dip & dunk @ 15 min interval
100% EtOH - room temp for 5 min
95% EtOH - room temp for 5 min
70% EtOH - room temp for 5 min
DI H2O - room temp for 5 min
Silver Nitrate (20 g) + DI H2O (400 ml) - room temp (in dark protected from
light) for 5 min
DI H2O rinse - room temp (protected from light) for 1 min
DI H2O rinse - room temp (protected from light) for 1 min
DI H2O rinse - room temp (protected from light) for 1 min
Sodium Carbonate (22.5 G) + DI H2O (337.5 ml) + 37% Formaldehyde (112.5 ml)
- room temp (protected from light) for 2 min
DI H2O rinse - room temp for 1 min
DI H2O rinse - room temp for 1 min
Sodium Thiosulfate (40 g) + Potassium Ferricyanide (2 g) + DI H2O (420 ml)
Solution - room temp for 30 sec(solution stable for 30-60 min after addition
of potassium ferricyanide)
Running Tap Water Rinse - 10 min
Counterstain w/ MacNeal's Tetrachrome (12 g) + DI H2O (600 ml) - room temp
for 6-8 min(stir continuously w/ heat @ 60C for 48 hours covered, then
filter with paper towel)
DI H2O rinse - room temp for 1 min
DI H2O rinse - room temp for 1 min
DI H2O rinse - room temp for 1 min
70% EtOH - room temp for 1 min
100% EtOH - room temp for 1 min
Xylenes - room temp for 3 min
Xylenes - room temp for 3 min
Coverslip w/ Eukitt


Hope this helps! Please feel free to contact me if you have any questions or
concerns.
Best Regards,
Jack

Jack L RatliffOwner, Ratliff Histology Consultants, LLCChairman, Hard Tissue
Committee - National Society for Histotechnology
(317) 281-1975jratl...@ratliffhistology.com LinkedIn Profile:
http://www.linkedin.com/profile/edit?trk=hb_tab_pro_top




> From: allison-malan...@uiowa.edu> To: histonet@lists.utsouthwestern.edu
> Date: Thu, 8 Nov 2012 22:52:42 +
> Subject: [Histonet] Stain for osteoporotic bone in Spurr plastic
> 
> Hi there -
> 
>   We are trying to stain osteoporotic bone that was embedded using Spurr
plastic.  We need to be able to differentiate osteoid from mineralized bone.
We have tried Gio's trichrome and Goldner's trichrome with no success, both
surface staining and on deplasticized slides.  We are thinking of trying Von
Kossa, does anyone have a recipe/protocol for this?  Or other types of
stains that you have had success with?  Thank you!
> 
> Allison Malandra, DVM
> University of Iowa, Bone Healing Research Lab
> 
> 
> 
> Notice: Th

RE: [Histonet] Dako vs Ventana IHC systems

[Patsy Ruegg]

I second Rene's suggestion, the Leica Bond instrument is my autostainer of
choice and I have not had any problems with their service, I find it to be
excellent.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax: 720-859-4110
pru...@ihctech.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Monday, October 29, 2012 9:27 AM
To: Joe W. Walker, Jr.; Terri Brown; Sebree Linda A; Burton, Lynn; Gloria
Tharp; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Dako vs Ventana IHC systems

Now that you are in the market to buy an autostainer, check the "BondMax"
originally from BioSystems (Australia) and now distributed by Leica
Microsystems.
I am sure you will like it.
René J.




From: "Joe W. Walker, Jr." 
To: Terri Brown ; Sebree Linda A
; "Burton, Lynn" ; Gloria
Tharp ; "histonet@lists.utsouthwestern.edu"
 
Sent: Monday, October 29, 2012 10:38 AM
Subject: RE: [Histonet] Dako vs Ventana IHC systems

Which Dako and Ventana machines are you all using?  We are in the market for
a new IHC stainer and the new Ventana BenckMark Ultra machine looks
interesting.

Joe W. Walker, Jr. MS, SCT(ASCP)CM
Anatomical Pathology Manager
Rutland Regional Medical Center
160 Allen Street, Rutland, VT 05701
P: 802.747.1790  F: 802.747.6525
NEW EMAIL: joewal...@rrmc.org
www.rrmc.org

Our Vision:
To be the Best Community Healthcare System in New England

Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet
Recognition® and the Governor's Award for Performance Excellence


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Brown
Sent: Monday, October 29, 2012 10:26 AM
To: Sebree Linda A; Burton, Lynn; Gloria Tharp;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Dako vs Ventana IHC systems

We had Ventana and changed to Dako.  The Dako phone tech support is
excellent and we never wait long for in house service.  Our
pathologists love the turnaround time with the open system and the stain
quality.

Terri H. Brown, HT (ASCP)
Pathology Laboratory Manager
Northside Hospital  Atlanta
terri.br...@northside.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda
A
Sent: Friday, October 26, 2012 4:52 PM
To: 'Burton, Lynn'; Gloria Tharp; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Dako vs Ventana IHC systems

I concur with Lynn; we've been dealing with Ventana 19 years.



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn
Sent: Friday, October 26, 2012 3:47 PM
To: Gloria Tharp; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Dako vs Ventana IHC systems

We have been dealing with Ventana for at least 10 years with very good
service and reliability. Their phone tech support is excellent and we have
never waited long for in house service.
Lynn Burton
Lab Associate
Animal Disease Lab
Galesburg, Il

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gloria Tharp
Sent: Friday, October 26, 2012 2:38 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Dako vs Ventana IHC systems

Does anyone have info about Dako vs Ventana IHC systems as to consistency
and reliability as far as turn around time on tech repair on site.  Thanks.





Gloria Tharp, BA, HTL(ASCP)

Director of Operations

PCA Southeast

gth...@pcasoutheast.com



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are not the intended recipient, you are hereby notified that you are not
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RE: [Histonet] Cassette Labeler

[Patsy Ruegg]

Yea and they welcomed responses by vendors and also wanted to hear from
users, this post seems legit to me, I would be glad to have this info.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax: 720-859-4110
pru...@ihctech.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango
Sent: Monday, October 29, 2012 11:21 AM
To: Norm Burnham
Cc: histonet@lists.utsouthwestern.edu; Shelly Coker
Subject: Re: [Histonet] Cassette Labeler

Hi Norm,

It sounds like he had something to contribute.  I don't think It shouldn't
matter where he works.  Someone else asked the question.

Mark
On Mon, Oct 29, 2012 at 10:00 AM, Norm Burnham
wrote:

> I didn't know that the Histonet site is being used as a medium for vendors
> to
> advertise their wares?  Your thoughts?
> Norm Burnham
>
> __
> Norm Burnham, MBA, MT(ASCP)
> Director, Anatomic Laboratory Operations
> ProPath - The Leader in Pathology Services
> 1355 River Bend Drive
> Dallas, TX 75247
> norm.burn...@propath.com
> 214.237.1602 Office
> 214.237.1802 Fax
> 214.709.7127 Cell
>
> To learn more about ProPath, please visit www.propath.com
>
>
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dustin
> Paul
> Campbell
> Sent: Monday, October 29, 2012 11:54 AM
> To: Kaye Ryan; Shelly Coker; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] Cassette Labeler
>
> To All,
>
> TBS offers a compatible cassette and slide labeler that interfaces with
> APEASY. We have several accounts using both systems.  If addition
> information
> is needed please contact me directly. dcampb...@trianglebiomedical.com
>
> Hope this helps,
>
>
>
>
> Dustin Campbell
> Service Technician
>
> 3014 Croasdaile Drive, Durham   NC  27705
>  p 919.384.9393   f 919.384.9595
> dcampb...@trianglebiomedical.com
>
> Visit us online at www.trianglebiomedical.com and follow us on
>
>
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kaye Ryan
> Sent: Monday, October 29, 2012 9:42 AM
> To: Shelly Coker; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] Cassette Labeler
>
> Please share that information with me also.
>
> Thanks,
> Kaye
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shelly
> Coker
> Sent: Saturday, October 27, 2012 10:14 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Cassette Labeler
>
> Anyone out there with AP Easy software that has a cassette labeler
> interfaced
> with your LIS software that you would recommend?  We are looking at adding
> one sometime next year.  (PS...while responses from vendors are welcome, I
> really want to hear from someone working with the instrument...)
>
> Thanks!
>
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>
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> named recipient and may contain information that is confidential or
> privileged. If you are not the intended recipient, you are hereby notified
> that any disclosure, copying, distribution, or use of the contents of this
> message is strictly prohibited. If you have received this message in
> error or are not the named recipient, please notify us immediately by
> contacting the sender at the electronic mail address noted above, and
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RE: [Histonet] IHC negative controls

[Patsy Ruegg]

So true!

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax: 720-859-4110
pru...@ihctech.net

-Original Message-
From: Morken, Timothy [mailto:timothy.mor...@ucsfmedctr.org] 
Sent: Monday, November 05, 2012 2:10 PM
To: Patsy Ruegg; 'Glen Dawson'; 'histonet'
Subject: RE: [Histonet] IHC negative controls

Could be, but my point, maybe not clear in my answer, is that you should
check with your accrediting agency before an inspection and not assume that
they will follow what CAP has said it will do.

Tim

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Monday, November 05, 2012 12:56 PM
To: Morken, Timothy; 'Glen Dawson'; 'histonet'
Subject: RE: [Histonet] IHC negative controls

The thing is in my opinion that having the backing of CAP on this issue is a
good argument to make to CLIA for why you are not doing negatives for
polymer based detection IHC work, at least you will have some documentation
to cover your decision.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax: 720-859-4110
pru...@ihctech.net
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken,
Timothy
Sent: Friday, October 26, 2012 9:45 AM
To: Glen Dawson; histonet
Subject: RE: [Histonet] IHC negative controls

" Can anyone tell me if JHACO & CLIA are deferring to CAP's judgment that a
negative control is not needed when utilizing a polymer detection?
 
I assume that this is the case, but I'd like to be sure."


Short answer: 

Don't bet the farm on it. Each enforces CLIA regulations but have different
methods of doing so. There is no reason to think that  JC or CAP will defer
to the other in any particular situation. They really don't have anything to
do with one another. My experience is that CAP is more lab-method oriented
while JC is more total-process (patient admission to final result )
oriented.  

Long answer: 

Let's clarify this. CLIA is the law administered by the Centers for Medicare
and Medicaid Services (CMS). The Joint Commission and CAP are two different,
independent accrediting agencies deemed by CMS to enforce the CLIA
regulations. CMS/ CLIA does not "defer" to CAP or JC, rather CMS deems JC
and CAP to be their agent to accredit laboratories according to the CLIA
law.  CAP and JC cannot enforce anything without CMS/CLIA approval. The fact
that CAP allows labs to leave out negative controls in certain situations
may be approved by CMS/ CLIA regulators, but it does not follow that CLIA or
JC inspectors will follow the same rational. JC is totally independent and
can make their own interpretation of the CLIA regulations, which CMS can
approve, even if they are different than what CAP allows, as long as it is
within the scope of the CLIA regulations. JC can simply interpret it
differently and require negative controls (I don't know if that is the case;
I haven't yet looked over the new checklist this year).  

Tim Morken
Department of Pathology
UC San Francisco Medical Center


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Glen Dawson
Sent: Friday, October 26, 2012 6:45 AM
To: histonet
Subject: [Histonet] IHC negative controls


All,
 
Can anyone tell me if JHACO & CLIA are deferring to CAP's judgement that a
negative control is not needed when utilizing a polymer detection?
 
I assume that this is the case, but I'd like to be sure.
 
Thank-you in advance,
 
Glen Dawson  BS, HT(ASCP), QIHC
Histology Technical Specialist
Janesville, WI
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RE: [Histonet] cutting bone with metal

[Patsy Ruegg]

Bone with metal implants will require ground sections prepared from methyl
methacrylate embedded samples, microtome sections even using tungsten
carbide knives and MMA embedding cannot usually be done in my experience.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax: 720-859-4110
pru...@ihctech.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy
Wenk
Sent: Friday, October 26, 2012 3:16 AM
To: histonet@lists.utsouthwestern.edu; Jennifer MacDonald
Subject: Re: [Histonet] cutting bone with metal 

Talk with Jack Ratliff, Chair of the NSH Hard Tissue Committee.

Jack L. Ratliff
615-236-4901
ratliffj...@gmail.com

The answer is Yes, histologic sections can be made, but need plastic resins 
(methyl methracylate or glycol methacrylate or something similar) and 
special microtomes and knives. If the researcher's lab doesn't do this 
technique, Jack can let him know who does, and the tissue can be sent out to

the specialty lab. Paraffin blocks on regular histology microtomes won't cut

it - literally and figuratively.

Peggy Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073

The opinions expressed are my own, and do not reflect on Beaumont Hospital.

-Original Message- 
From: Jennifer MacDonald
Sent: Thursday, October 25, 2012 11:38 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cutting bone with metal

I have been asked the following question.  I do not have an answer and was
hoping someone in the Histonet community did.
Thanks.

There is a researcher who is doing orthopedic procedures on broken rat
tibias. The researcher is repairing the tibias with metal rods or
plates.not sure which (and the doctor isn't sure what kind of metal
either). The researcher wants to know if it is possible to make histologic
sections of the repaired tibias with the metal intact 


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RE: [Histonet] IHC negative controls

[Patsy Ruegg]

The thing is in my opinion that having the backing of CAP on this issue is a
good argument to make to CLIA for why you are not doing negatives for
polymer based detection IHC work, at least you will have some documentation
to cover your decision.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax: 720-859-4110
pru...@ihctech.net
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken,
Timothy
Sent: Friday, October 26, 2012 9:45 AM
To: Glen Dawson; histonet
Subject: RE: [Histonet] IHC negative controls

" Can anyone tell me if JHACO & CLIA are deferring to CAP's judgment that a
negative control is not needed when utilizing a polymer detection?
 
I assume that this is the case, but I'd like to be sure."


Short answer: 

Don't bet the farm on it. Each enforces CLIA regulations but have different
methods of doing so. There is no reason to think that  JC or CAP will defer
to the other in any particular situation. They really don't have anything to
do with one another. My experience is that CAP is more lab-method oriented
while JC is more total-process (patient admission to final result )
oriented.  

Long answer: 

Let's clarify this. CLIA is the law administered by the Centers for Medicare
and Medicaid Services (CMS). The Joint Commission and CAP are two different,
independent accrediting agencies deemed by CMS to enforce the CLIA
regulations. CMS/ CLIA does not "defer" to CAP or JC, rather CMS deems JC
and CAP to be their agent to accredit laboratories according to the CLIA
law.  CAP and JC cannot enforce anything without CMS/CLIA approval. The fact
that CAP allows labs to leave out negative controls in certain situations
may be approved by CMS/ CLIA regulators, but it does not follow that CLIA or
JC inspectors will follow the same rational. JC is totally independent and
can make their own interpretation of the CLIA regulations, which CMS can
approve, even if they are different than what CAP allows, as long as it is
within the scope of the CLIA regulations. JC can simply interpret it
differently and require negative controls (I don't know if that is the case;
I haven't yet looked over the new checklist this year).  

Tim Morken
Department of Pathology
UC San Francisco Medical Center


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Glen Dawson
Sent: Friday, October 26, 2012 6:45 AM
To: histonet
Subject: [Histonet] IHC negative controls


All,
 
Can anyone tell me if JHACO & CLIA are deferring to CAP's judgement that a
negative control is not needed when utilizing a polymer detection?
 
I assume that this is the case, but I'd like to be sure.
 
Thank-you in advance,
 
Glen Dawson  BS, HT(ASCP), QIHC
Histology Technical Specialist
Janesville, WI
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RE: [Histonet] CD31 for FFPE IHC on Mouse

[Patsy Ruegg]

Rat anti mouse cd31 from Dionova is the way to go it is better than all the
others.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax: 720-859-4110
pru...@ihctech.net
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Elliott
Sent: Friday, October 19, 2012 3:45 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CD31 for FFPE IHC on Mouse

Amy
Can you please share the responses you got with the rest of us
Thanks
Mark
 

Message: 8
Date: Thu, 18 Oct 2012 16:21:55 -0400
From: "Amy Porter" 
Subject: RE: [Histonet] CD31 for FFPE Immunohistochemistry on Mouse
Model
To: "'Amy Porter'" ,"'Histonet'"

Message-ID: <003101cdad6e$3792fc80$a6b8f580$@edu>
Content-Type: text/plain;charset="US-ASCII"

Thanks to all for responses..looks like most roads lead to once place
which is spectacular!!  Just what I needed.  Amy

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Porter
Sent: Thursday, October 18, 2012 3:36 PM
To: 'Histonet'
Subject: [Histonet] CD31 for FFPE Immunohistochemistry on Mouse Model

Anyone out there have CD31 working well on FFPE samples for Mouse Samples??
I know this might be a long shot, however I haven't looked for anything on
this in quite awhile.



Amy S. Porter, HT(ASCP) QIHC

Michigan State University

Investigative HistoPathology Laboratory

William S. Spielman, Ph.D. - Director

Patricia K. Senagore, M.D. - Consulting Pathologist

Department of Physiology / Human Pathology

Biomedical Physical Sciences Building 

567 Wilson Road - Room 2133

East Lansing, MI  48824-3320

Phone:  517-884-5026

Fax:  517-432-1368

port...@msu.edu

www.humanpathology.msu.edu




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[Histonet] FW: Announcing the 7th international retreat of applied IHC and molecular pathology (AIMP)

[Patsy Ruegg]

I am resending this as some have said the links are not working, here is the
actual link if it does not work from the text
http://pathlearning.com/Pathology_Learning_Center/Course_Schedule.html 

 

Patsy Ruegg, HT(ASCP)QIHC

Ruegg IHC Consulting, LLC

40864 Arkansas Ave

Bennett, CO 80102

Phone: 303-644-4538

Fax: 720-859-4110

 <mailto:pru...@ihctech.net> pru...@ihctech.net

  _  

From: Hadi Yaziji, MD [mailto:i...@pathlearning.com] 
Sent: Friday, October 05, 2012 8:03 AM
To: Patsy Ruegg
Subject: Announcing the 7th international retreat of applied IHC and
molecular pathology (AIMP)

 

Dear colleagues,

 

It is our honor and pleasure to announce the 7th Annual International
Retreat on Applied Immunohistochemistry and Molecular Pathology (AIMP), from
February 3-7, 2013, in the sunny and beautiful Miami (Coral Gables), FL. The
reason we have been offering this retreat for six years (and hopefully many
more years to come) is because we maintained focus on the issues that matter
the most to the practicing pathologist. In the way of example , the 7th
annual retreat will continue to focus on the following aspects of IHC and
molecular applications in our practice:

1. Among the >2000 new antibodies, can you offer a short list of new
antibodies that I can benefit from their utility in surgical pathology?
2. What type of IHC platform should we bring to our pathology lab?
3. How can we effectively apply QA measures into our IHC lab and molecular
lab?
4. What kind of impact do the ASCO/CAP guidelines have on our gross room
workflow and documentation?
5. What do I need to do to ensure our lab is compliant with these
guidelines, and compliant with other CAP and CLIA requirements?
6. What kind of FISH or molecular assays could our lab benefit from bringing
in-house?
7. What are the most common molecular assays in oncologic pathology that I
should be familiar with?
8. How do I build a molecular lab in my hospital?
9. Can you show me real - life examples of the staining pattern of
antibodies I use in my practice?

10. What are the clinical applications of the molecular oncology assays, and
what is the biology behind the testing and treatment?

11. What's the best IHC panel to properly classify lung cancer?

12. What's the best IHC panel to properly work up undifferentiated malignant
neoplasm

13. What's the best IHC panel to properly work up metastatic carcinoma of
unknown primary site?

13. What's the best IHC panel for mesothelioma vs. carcinoma?

14. How do I workup germ cell tumors by IHC?

15. How do I do IHC on cytology preparations?

16. What are the cancer predictive/prognostic assays that are not based on
IHC, ISH or FISH?

17. What are the reimbursement issues facing payments for IHC, FISH and
molecular assays?


In essence, there is nothing that you deal with in your day-to-day surgical
pathology practice as far as ancillary testing is concerned that is not
going to be discussed at this retreat in some way, shape or form. We go
straight to the bottom line without presenting to you any non-evidence based
science that is not going to affect your practice of surgical pathology. 


We constructed the educational program to address the above practical
questions, as you can note from perusing the Schedule page. We assembled a
group of the most experienced colleagues in the field, including Rich
Cartun, Rich Eisen, David Hicks, Todd Barry, Jason Hornick, Savitri
Krishnamurthy, and myself. For the detailed schedule, please click here.
<http://pathlearning.dmanalytics2.com/click?u=http%3A%2F%2Fpathlearning.com%
2FPathology_Learning_Center%2FCourse_Schedule.html&i=1&d=98Y490Y3-0468-452X-
81Z0-61VZX0W1XVW1&e=pruegg%40ihctech.net&a=6X62VV8U-5X77-4UY3-Z41U-5VU08WZW7
625> 

On average, 40% of attendees are repeat attendees. Our colleagues who
attended the first five courses truly enjoyed this event. In fact, many of
them described it as "the most practical CME pathology course I've ever
attended". We hope you will enjoy the upcoming course. It is very exciting
and we look forward to seeing you there.

Please click here
<http://pathlearning.dmanalytics2.com/click?u=http%3A%2F%2Fwww.pathlearning.
com%2F&i=2&d=98Y490Y3-0468-452X-81Z0-61VZX0W1XVW1&e=pruegg%40ihctech.net&a=6
X62VV8U-5X77-4UY3-Z41U-5VU08WZW7625> Â for detailed information of the
program and how to register. This year, the ballroom can only accommodate a
maximum of 175 participants in classroom seating. We recommend that you
register fairly soon to guarantee a spot.

Best regards,

Hadi Yaziji, MD and Rich Eisen, MD
Course Directors

 

 


 




 

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<https://w

RE: [Histonet] Process Improvements

[Patsy Ruegg]

Fawn,

Could you not use something like the number of recuts, reembeds, staining
repeats for something like that?  For each parameter u could list
suggestions for improving those repeat test to lower the numbers from month
to month. 

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax: 720-859-4110
pru...@ihctech.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Saturday, October 06, 2012 2:36 AM
To: 'Fawn Bomar'
Cc: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] Process Improvements

I would recommend data, that are available through lab-IT. It is a kind of
frustrating and time-consuming, if you have to monitor daily a large number
of figures. 
E.g. we have an "incoming" time and a "ready of report" time in our system,
that tells us the mean-time of histology testing.

Or for medical results, the system should have the possibility to monitor
the single parameters (e.g. estrogen positivity). So one can create a report
out of IT.
Everything you put into the system, should give you a report.

And you should choose parameters, that can really be altered by yourself, if
you are not content with the result.
Often we see a problem, but everyone says: there's nothing we can change...
that's out of our control.

Gudrun


-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Fawn Bomar
Gesendet: Freitag, 05. Oktober 2012 16:40
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Process Improvements

Hi everyone,



My new manager would like me to come up with a list of process/performance
improvements in the pathology department that can be measured on a
monthly/quarterly basis, such as they do for other departments in the
hospital, i.e. the ER measures the number of patient falls with <5 per
quarter being their goal.  I need advice on what to measure for our
histology/pathology/cytology departments.  Does anyone have any suggestions?



Thank you

Fawn
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RE: [Histonet] RE: Mitochondiral membrane

[Patsy Ruegg]

I have use a mitochondria marker from Dako but it is cytoplasmic not on the
membrane.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax: 720-859-4110
pru...@ihctech.net
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth
Chlipala
Sent: Tuesday, October 09, 2012 8:55 AM
To: 'fujis...@mskcc.org'; Histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Mitochondiral membrane

Sho



There is a mitochondria marker for human tissue samples they are from Novus,
not sure if it's a membrane marker or not.  Good Luck!


Protocol:  Mouse anti Mitochondria
Clone: MTC02
Vendor: Novus Biologicals
Catalog Number: NB600-556
Species: Mouse
Isotype: IgG21

Spec Sheet: Novus
Mito<http://www.novusbio.com/data_sheet/index/NB600-556&prev_module=search>



Liz



Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC

Manager

Premier Laboratory, LLC

PO Box 18592

Boulder, CO 80308-1592

(303) 682-3949 office

(303) 682-9060 fax

(303) 881-0763 cell

www.premierlab.com



Ship to address:



1567 Skyway Drive, Unit E

Longmont, CO 80504



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
fujis...@mskcc.org
Sent: Tuesday, October 09, 2012 8:43 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mitochondiral membrane



Dear all-



I have a user looking to label mitochondrial membrane.



Can anybody recommend a commercial antibody? I'm guessing one of the TOM
complex proteins would be a good target.



Thank you in advance.





Sho Fujisawa

Molecular Cytology Core Facility

Memorial Sloan-Kettering Cancer Center

415 East 68th St. ZRC-1840

New York, NY 10065

(646) 888-2186, x2186







 =



 Please note that this e-mail and any files transmitted from

 Memorial Sloan-Kettering Cancer Center may be privileged, confidential,

 and protected from disclosure under applicable law. If the reader of

 this message is not the intended recipient, or an employee or agent

 responsible for delivering this message to the intended recipient,

 you are hereby notified that any reading, dissemination, distribution,

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 and deleting this message, any attachments, and all copies and backups

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[Histonet] FW: Announcing the 7th international retreat of applied IHC and molecular pathology (AIMP)

[Patsy Ruegg]

 

 

Patsy Ruegg, HT(ASCP)QIHC

Ruegg IHC Consulting, LLC

40864 Arkansas Ave

Bennett, CO 80102

Phone: 303-644-4538

Fax: 720-859-4110

 <mailto:pru...@ihctech.net> pru...@ihctech.net

  _  

From: Hadi Yaziji, MD [mailto:i...@pathlearning.com] 
Sent: Friday, October 05, 2012 8:03 AM
To: Patsy Ruegg
Subject: Announcing the 7th international retreat of applied IHC and
molecular pathology (AIMP)

 

Dear colleagues,

 

It is our honor and pleasure to announce the 7th Annual International
Retreat on Applied Immunohistochemistry and Molecular Pathology (AIMP), from
February 3-7, 2013, in the sunny and beautiful Miami (Coral Gables), FL. The
reason we have been offering this retreat for six years (and hopefully many
more years to come) is because we maintained focus on the issues that matter
the most to the practicing pathologist. In the way of example , the 7th
annual retreat will continue to focus on the following aspects of IHC and
molecular applications in our practice:

1. Among the >2000 new antibodies, can you offer a short list of new
antibodies that I can benefit from their utility in surgical pathology?
2. What type of IHC platform should we bring to our pathology lab?
3. How can we effectively apply QA measures into our IHC lab and molecular
lab?
4. What kind of impact do the ASCO/CAP guidelines have on our gross room
workflow and documentation?
5. What do I need to do to ensure our lab is compliant with these
guidelines, and compliant with other CAP and CLIA requirements?
6. What kind of FISH or molecular assays could our lab benefit from bringing
in-house?
7. What are the most common molecular assays in oncologic pathology that I
should be familiar with?
8. How do I build a molecular lab in my hospital?
9. Can you show me real - life examples of the staining pattern of
antibodies I use in my practice?

10. What are the clinical applications of the molecular oncology assays, and
what is the biology behind the testing and treatment?

11. What's the best IHC panel to properly classify lung cancer?

12. What's the best IHC panel to properly work up undifferentiated malignant
neoplasm

13. What's the best IHC panel to properly work up metastatic carcinoma of
unknown primary site?

13. What's the best IHC panel for mesothelioma vs. carcinoma?

14. How do I workup germ cell tumors by IHC?

15. How do I do IHC on cytology preparations?

16. What are the cancer predictive/prognostic assays that are not based on
IHC, ISH or FISH?

17. What are the reimbursement issues facing payments for IHC, FISH and
molecular assays?


In essence, there is nothing that you deal with in your day-to-day surgical
pathology practice as far as ancillary testing is concerned that is not
going to be discussed at this retreat in some way, shape or form. We go
straight to the bottom line without presenting to you any non-evidence based
science that is not going to affect your practice of surgical pathology. 


We constructed the educational program to address the above practical
questions, as you can note from perusing the Schedule page. We assembled a
group of the most experienced colleagues in the field, including Rich
Cartun, Rich Eisen, David Hicks, Todd Barry, Jason Hornick, Savitri
Krishnamurthy, and myself. For the detailed schedule, please click here.
<http://pathlearning.dmanalytics2.com/click?u=http%3A%2F%2Fpathlearning.com%
2FPathology_Learning_Center%2FCourse_Schedule.html&i=1&d=98Y490Y3-0468-452X-
81Z0-61VZX0W1XVW1&e=pruegg%40ihctech.net&a=6X62VV8U-5X77-4UY3-Z41U-5VU08WZW7
625> 

On average, 40% of attendees are repeat attendees. Our colleagues who
attended the first five courses truly enjoyed this event. In fact, many of
them described it as "the most practical CME pathology course I've ever
attended". We hope you will enjoy the upcoming course. It is very exciting
and we look forward to seeing you there.

Please click here
<http://pathlearning.dmanalytics2.com/click?u=http%3A%2F%2Fwww.pathlearning.
com%2F&i=2&d=98Y490Y3-0468-452X-81Z0-61VZX0W1XVW1&e=pruegg%40ihctech.net&a=6
X62VV8U-5X77-4UY3-Z41U-5VU08WZW7625> Â for detailed information of the
program and how to register. This year, the ballroom can only accommodate a
maximum of 175 participants in classroom seating. We recommend that you
register fairly soon to guarantee a spot.

Best regards,

Hadi Yaziji, MD and Rich Eisen, MD
Course Directors

 

 


 




 

 <http://directmailmac.com> Direct Mail for Mac


 


 

This email is powered by Direct Mail for Mac.  <http://directmailmac.com>
Learn More
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61VZX0W1XVW1&e=pru...@ihctech.net> Report Spam 


 


 

 
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[Histonet] old tissue processor reagent bottles

[Patsy Ruegg]

Can someone help me find bottles for an old tissue processor IMS LX 120
Fisher Scientific, the bottles got separated from the machine.

 

Cheers,

Patsy

 

Patsy Ruegg, HT(ASCP)QIHC

Ruegg IHC Consulting, LLC

40864 Arkansas Ave

Bennett, CO 80102

Phone: 303-644-4538

Fax: 720-859-4110

 <mailto:pru...@ihctech.net> pru...@ihctech.net

 

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RE: [Histonet] Cd31

[Patsy Ruegg]

Unfortunately we do not have an anti rat cd31 like the rat anti ms one, so
what I have used in rat tissue is either CD34 (not that specific to endo
cells, stains a lot of other stuff), SMA or F8 (these two pick up the more
mature vessels but not the early ones), we need an anti rat CD31 like the
anti ms one from Dianova.  Years ago SC had a good cd31 made in a rabbit or
goat that worked well in ms or rat tissue but the rabbit died or something
and we have not gotten another good one yet for rat tissue that I know of???

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of helen.ilsley
Sent: Saturday, September 15, 2012 2:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cd31

Hi

Which endothelial marker would you use for rat tissue?
I am really interested to see what you all say.
Thanks in advance
Helen

Sent from my BlackBerryR wireless device


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RE: [Histonet] Tissue grossing

[Patsy Ruegg]

You need to have a minimum of an associates degree and then go thru training
by a pathologist to qualify to do grossing.

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen
martin 
Sent: Saturday, September 15, 2012 2:27 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Tissue grossing

Hi all,
How does one become qualified to gross tissue? A lot of the jobs I have been
looking at call for being qualified to gross tissue, where I work right now
the residents and Pathology assistants are the ones who gross the tissue. 
Thanks in advance for any help!
Kristen Martin

Sent from my Verizon Wireless BlackBerry


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RE: [Histonet] endothelial cell marker

[Patsy Ruegg]

By far the best ab for endothelial cells in mouse tissue is rat anti CD31
from Dianovo, it picks up the really early forming vessels better than any
other cd31 or F8 or SMA I have ever used.  We like it in AP/red.

Regards,
Patsy


Hi,
 My favorite by far is VonWillebrandt (spelling?) factor VIII. Dako has
a really good one (Cat# A0082) that works great in mouse tissue. CD31 is OK
but it is really finicky. CD34 works but F8 is easier and more reliable.

Amos


On Wed, Sep 5, 2012 at 1:01 PM,
wrote:

> Message: 5
> Date: Wed, 5 Sep 2012 10:04:58 +
> From: "Bruijntjes, J.P. (Joost)" 
> Subject: [Histonet] endothelial cell marker
> To: "Histonet@lists.utsouthwestern.edu"
> 
> Message-ID:
> 
> Content-Type: text/plain; charset="us-ascii"
>
> Hi all
>
> Is anyone of you familiar with an antibody directed against endothelial
> cells which can be applied on FFPE mouse tissues?
>
> Best regards
> Joost Bruijntjes
>
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[Histonet] cell membrane

[Patsy Ruegg]

Dear Histochemist,

 

Does anyone know of a stain or histochemical reaction which will exclusively
stain cell membrane and no other parts of the cell or other tissue
components?

 

Best regards,

Patsy

 

 

 

Patsy Ruegg, HT(ASCP)QIHC
Director of Histology and IHC

IHCtech a subsidiary of Flagship Bio-Sciences, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80045 

P-720-859-4060
F-720-859-4110
email  <mailto:pru...@ihctech.net> pru...@ihctech.net &
email  <mailto:pru...@flagshipbio.com> pru...@flagshipbio.com 

web site  <http://www.ihctech.net> www.ihctech.net

 

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RE: [Histonet] Has anyone done ISH with fairly large FFPE sections?

[Patsy Ruegg]

We use a Tunnel kit from Chemicon and apply about 30ul of probe with a cs on
top, look into buying just the probe which is what goes first, for our
Chemicon kit we buy several vials of probe before we have to buy the entire
kit again.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax: 720-859-4110
pru...@ihctech.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lewis,
Patrick
Sent: Friday, August 10, 2012 9:57 AM
To: 'Histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Has anyone done ISH with fairly large FFPE sections?

Hi Everyone,

I am going to do some ISH for EBER  on FFPE sections, and I have some
concerns on the logistics.

The kits I am looking at are for 50 rxns, and it seems like they expect to
use 20 uL probe per rxn.

My sections are rather large, covering about 2/3 of a standard slide.

How many uL will it take to make sure I get good coverage and have enough to
avoid drying out the slide?

I plan to cover slip them and use a humidity chamber, but even still, I am
worried that with such a small volume it don't be enough.

These kits are expensive, +$900.00 each and if I have to double/triple my
rxn volume that would use up the kit in a hurry.

I haven't fully familiarized myself with the protocol yet, so maybe my
concerns are unjustified, but if anyone has done some ISH, I'd appreciate
any advice you'd care to give.

Thanks

Patrick.

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RE: [Histonet] sections falling off in day 2 ISH (Megha Kumar)

[Patsy Ruegg]

I would use good charged slides like VWR Superfrost Plus or Fisher + slides
and try to cut the sections thinner, sections thicker than 4-6 microns fall
off easier.  It is also important to airdry slides completely before baking
them, we bake at 60dc for an hour to overnight in an oven after airdrying.
For ish we do it manually on a hot plate and put a glass coverslip over the
section to keep it from drying out during high temp long incubations
required for ISH. 

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax: 720-859-4110
pru...@ihctech.net
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Therese C.
Thinnes
Sent: Friday, August 10, 2012 12:05 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] sections falling off in day 2 ISH (Megha Kumar)


Hi Megha, Not sure why your sections are falling off. When I did ISH I  used
Colorfrost Plus Slides. I would cut at 4 microns-mouse or human.  I would
dry them at room temp a few hours followed by 55 C for an hour. I would use
the slides the next day or next week, never the same day. On day 2 my SSC
was 60 C and I used a stir bar for 2 hours on slow stirring. Rarely did I
lose any sections.
Terri Thinnes
Research Assistant
The Scripps Research Institute
La Jolla, CA




Message: 13
Date: Fri, 10 Aug 2012 14:40:30 +0530
From: Megha Kumar 
Subject: [Histonet] sections falling off in day 2 ISH
To: histonet@lists.utsouthwestern.edu
Message-ID:

Content-Type: text/plain; charset=ISO-8859-1

Hello everyone!
When I subject murine skin and intestine paraffin sections (7-10microns) to
ISH protocol, the sections fall off on day 2 when I add the SSC. The
sections are taken on ploy lysine coated slides and remain on the slides
when I do other protocols such as antibody staining. Can anyone suggest me
why this is happening? How to prevent the slides from falling off on day 2?
Thank you for all the help!
regards
Megha



*

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RE: [Histonet] disposable blade holder

[Patsy Ruegg]

Yes it fits on the microtome where the permanent blades go for older
microtomes that do not have disposable blade holding stages. 

Patsy Ruegg, HT(ASCP)QIHC
Director of Histology and IHC
IHCtech a subsidiary of Flagship Bio-Sciences, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80045 
P-720-859-4060
F-720-859-4110
email pru...@ihctech.net &
email pru...@flagshipbio.com 
web site www.ihctech.net
 

This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.

-Original Message-
From: MaryK Mendell [mailto:kmend...@goldbergmd.net] 
Sent: Monday, July 23, 2012 6:26 AM
To: Patsy Ruegg
Subject: RE: [Histonet] disposable blade holder

Patsy
are you talking about on the microtome it self.  
Kate Mendell
Histopathology/Lab Manager

HOWARD S. GOLDBERG, M.D., INC
990 Paradise Road
Swampscott, MA  01907
TEL:  781.595.0151
FAX:  781.592.6780
kmend...@goldbergmd.net
www.cosmesticdermcenter.com
PRIVACY NOTICE: This e-mail message may contain confidential patient or
other information belonging to the sender that is legally privileged.  This
information is intended only for the use of the individual or authorized
entity named above. The authorized recipient of this patient or other
confidential information is prohibited from disclosing the information to
any other party.  If you have received this message in error, please notify
the sender immediately and delete.  Please keep any information you may have
viewed confidential.

From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
[pru...@ihctech.net]
Sent: Sunday, July 22, 2012 2:33 PM
To: Histonet@lists.utsouthwestern.edu
Cc: 'Sherri Saturley'
Subject: [Histonet] disposable blade holder

We are looking for the disposable blade holder for low profile blades that
fit in microtomes like the Leica 1510 where permanent blades used to be
held.  We have two of these but they are both for high profile blades and we
would prefer to use low profile disposable blades.  Does anyone have one of
these sitting around used we could buy from you or if there are vendors
selling used ones please feel free to contact us.



Regards,

Patsy and Sherri



Patsy Ruegg, HT(ASCP)QIHC
Director of Histology and IHC

IHCtech a subsidiary of Flagship Bio-Sciences, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80045

P-720-859-4060
F-720-859-4110
email  <mailto:pru...@ihctech.net> pru...@ihctech.net &
email  <mailto:pru...@flagshipbio.com> pru...@flagshipbio.com

web site  <http://www.ihctech.net> www.ihctech.net




This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
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[Histonet] disposable blade holder

[Patsy Ruegg]

We are looking for the disposable blade holder for low profile blades that
fit in microtomes like the Leica 1510 where permanent blades used to be
held.  We have two of these but they are both for high profile blades and we
would prefer to use low profile disposable blades.  Does anyone have one of
these sitting around used we could buy from you or if there are vendors
selling used ones please feel free to contact us.

 

Regards,

Patsy and Sherri

 

Patsy Ruegg, HT(ASCP)QIHC
Director of Histology and IHC

IHCtech a subsidiary of Flagship Bio-Sciences, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80045 

P-720-859-4060
F-720-859-4110
email  <mailto:pru...@ihctech.net> pru...@ihctech.net &
email  <mailto:pru...@flagshipbio.com> pru...@flagshipbio.com 

web site  <http://www.ihctech.net> www.ihctech.net

 


This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.

 

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RE: [Histonet] ER, Clone SP1

[Patsy Ruegg]

I need to correct my statement about ER Sp1 being sold in Europe by Leica
after taking it off the market in the USA, I was confusing this ab with the
HER2 CB11 that Leica was still selling in Europe and not the USA, they had
to take it off the market in US to get FDA approval which they apparently
just got.  I apologize for this misinformation, I am getting old and can't
keep things straight anymore, and it was the HER2 CB11 I stock piled not the
ER SP1.

Best regards,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
Ruegg Consulting
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax: 720-859-4110
pru...@ihctech.net

-Original Message-
From: Patsy Ruegg [mailto:pru...@ihctech.net] 
Sent: Thursday, July 12, 2012 8:45 PM
To: 'Morken, Timothy'; 'Kim Donadio'
Cc: ''
Subject: RE: [Histonet] ER, Clone SP1

Yea you are right Tim, I just wonder what ever happened to trying to get the
best ab for the patient, SP1 is in my opinion the best ER ab but now the
patient suffers because we can't use what is best for them, there is
something wrong with that picture.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg Consulting
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax: 720-859-4110
pru...@ihctech.net

-Original Message-
From: Morken, Timothy [mailto:timothy.mor...@ucsfmedctr.org] 
Sent: Thursday, July 12, 2012 4:05 PM
To: Kim Donadio; Patsy Ruegg
Cc: 
Subject: RE: [Histonet] ER, Clone SP1

It is most likely licensing issues between companies. A company may carry it
under license from another company but the terms change and the licensee
either loses the license (the other company won't license it), can't afford
it or other issues. 

For example, when I worked in the antibody industry  we had several
antibodies for a certain target but another company got a patent on the
target and all antibodies to the target (still not sure why that is
possible!). They finally decided to enforce their patent and wanted a
licensing fee that was higher than our annual sales of all the antibodies
put together. Obviously not a viable deal for our company. 

Tim Morken

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Donadio
Sent: Thursday, July 12, 2012 2:55 PM
To: Patsy Ruegg
Cc: 
Subject: Re: [Histonet] ER, Clone SP1

I'm curious as to why all if a sudden some of these can be sold else where
and not in the USA. Is it a FDA issue? 

Sent from my iPhone

On Jul 12, 2012, at 5:45 PM, "Patsy Ruegg"  wrote:

>> From what I understand the SP1 Er clone has also been discontinued in 
>> USA by
> Leica/Novacastra, I am told it is still being sold by Leica in Europe?  
> Must be another licensing issue because some are s greedy.  I knew 
> this was going to happen and stock piled some of the ER SP1, but it 
> won't last forever.
> 
> Patsy Ruegg, HT(ASCP)QIHC
> Ruegg Consulting
> 40864 Arkansas Ave
> Bennett, CO 80102
> Phone: 303-644-4538
> Fax: 720-859-4110
> pru...@ihctech.net
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Leigh 
> York
> Sent: Thursday, July 12, 2012 1:06 PM
> To: Histonet@lists.utsouthwestern.edu
> Subject: [Histonet] ER, Clone SP1
> 
> Dako is going to discontinue the ER , SP1 clone.  We really want to 
> use the
> SP1 clone, but do not want to use an ASR.  We can get it from Ventana 
> only we are told.  Can anyone elaborate on how they are handling this 
> in their lab or if they have switched to an ASR?
> 
> Thanks,
> 
> Leigh York
> ___
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> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
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RE: [Histonet] ER, Clone SP1

[Patsy Ruegg]

Yea you are right Tim, I just wonder what ever happened to trying to get the
best ab for the patient, SP1 is in my opinion the best ER ab but now the
patient suffers because we can't use what is best for them, there is
something wrong with that picture.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg Consulting
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax: 720-859-4110
pru...@ihctech.net

-Original Message-
From: Morken, Timothy [mailto:timothy.mor...@ucsfmedctr.org] 
Sent: Thursday, July 12, 2012 4:05 PM
To: Kim Donadio; Patsy Ruegg
Cc: 
Subject: RE: [Histonet] ER, Clone SP1

It is most likely licensing issues between companies. A company may carry it
under license from another company but the terms change and the licensee
either loses the license (the other company won't license it), can't afford
it or other issues. 

For example, when I worked in the antibody industry  we had several
antibodies for a certain target but another company got a patent on the
target and all antibodies to the target (still not sure why that is
possible!). They finally decided to enforce their patent and wanted a
licensing fee that was higher than our annual sales of all the antibodies
put together. Obviously not a viable deal for our company. 

Tim Morken

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Donadio
Sent: Thursday, July 12, 2012 2:55 PM
To: Patsy Ruegg
Cc: 
Subject: Re: [Histonet] ER, Clone SP1

I'm curious as to why all if a sudden some of these can be sold else where
and not in the USA. Is it a FDA issue? 

Sent from my iPhone

On Jul 12, 2012, at 5:45 PM, "Patsy Ruegg"  wrote:

>> From what I understand the SP1 Er clone has also been discontinued in 
>> USA by
> Leica/Novacastra, I am told it is still being sold by Leica in Europe?  
> Must be another licensing issue because some are s greedy.  I knew 
> this was going to happen and stock piled some of the ER SP1, but it 
> won't last forever.
> 
> Patsy Ruegg, HT(ASCP)QIHC
> Ruegg Consulting
> 40864 Arkansas Ave
> Bennett, CO 80102
> Phone: 303-644-4538
> Fax: 720-859-4110
> pru...@ihctech.net
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Leigh 
> York
> Sent: Thursday, July 12, 2012 1:06 PM
> To: Histonet@lists.utsouthwestern.edu
> Subject: [Histonet] ER, Clone SP1
> 
> Dako is going to discontinue the ER , SP1 clone.  We really want to 
> use the
> SP1 clone, but do not want to use an ASR.  We can get it from Ventana 
> only we are told.  Can anyone elaborate on how they are handling this 
> in their lab or if they have switched to an ASR?
> 
> Thanks,
> 
> Leigh York
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> ___
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RE: [Histonet] ER, Clone SP1

[Patsy Ruegg]

>From what I understand the SP1 Er clone has also been discontinued in USA by
Leica/Novacastra, I am told it is still being sold by Leica in Europe?  Must
be another licensing issue because some are s greedy.  I knew this was
going to happen and stock piled some of the ER SP1, but it won't last
forever. 

Patsy Ruegg, HT(ASCP)QIHC
Ruegg Consulting
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax: 720-859-4110
pru...@ihctech.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Leigh York
Sent: Thursday, July 12, 2012 1:06 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] ER, Clone SP1

Dako is going to discontinue the ER , SP1 clone.  We really want to use the
SP1 clone, but do not want to use an ASR.  We can get it from Ventana only
we are told.  Can anyone elaborate on how they are handling this in their
lab or if they have switched to an ASR?  

Thanks,

Leigh York
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RE: [Histonet] Armadillos living at AFIP

[Patsy Ruegg]

Is that what you get to do in retirement, I am working on my second one,
hopefully I can start ignoring now.

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
nmhi...@comcast.net
Sent: Monday, June 11, 2012 1:53 PM
To: Rene J Buesa
Cc: histonet@lists.utsouthwestern.edu; Bob Richmond; Shirley A. Powell
Subject: Re: [Histonet] Armadillos living at AFIP



Rene - Those were STUFFED armadillos that had escaped from the Smithsonian! 
The real armadillo work (with leprosy) is still going on in Hawaii ('cause I
got involved in that for a very short period of time in the Olde Days).  If
you want an armadillo - ask someone that lives in Texas and I'm sure they'd
be happy to round one up for you.  Watch them claws, though. 



Retirement... aaah...time to annoy people! 



- Original Message -


From: "Rene J Buesa"  
To: "Shirley A. Powell" , "Jay Lundgren"
, "Bob Richmond"  
Cc: histonet@lists.utsouthwestern.edu 
Sent: Monday, June 11, 2012 11:02:45 AM 
Subject: Re: [Histonet] Armadillos living at AFIP 

What happened to the armadillos in AFIP? 
Who knows what happened to them when AFIP CLOSED! 
Didn't you know that? 
René J. 


 
From: Shirley A. Powell  
To: Jay Lundgren ; Bob Richmond
 
Cc: "histonet@lists.utsouthwestern.edu"  
Sent: Monday, June 11, 2012 12:54 PM 
Subject: RE: [Histonet] Re: best controls 

I want to know what happened to all the equipment there, especially the
large Sartorius Microtome that was in the bone lab.  If anyone has that
information please email me.  

Shirley 

-Original Message- 
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren

Sent: Monday, June 11, 2012 1:34 AM 
To: Bob Richmond 
Cc: histonet@lists.utsouthwestern.edu 
Subject: Re: [Histonet] Re: best controls 

I wonder what happened to all the leprous armadillos living at AFIP? 

              Heeere little feller 

                          Jay A. Lundgren, M.S., HTL (ASCP)
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RE: [Histonet] Unregistered techs

[Patsy Ruegg]

I am sorry but I must be living in dream world, in the last 9 years I have 
trained at least 4 people in histology who passed the Ht or the HTL exam and I 
would put them up against any histotech for knowing what stains the nucleus, 
special stains, as well as being really well trained (not just to chose the 
right bar code) in IHC, to do IHC manually and trouble shoot problems that do 
go wrong with autostainers .  in my community ASCP certification and even more 
than that who trained you and how you were trained matters.

 

Regards,

Patsy

 

Patsy Ruegg, HT(ASCP)QIHC

IHCtech

12635 Montview Blvd. Ste.215

Aurora, CO 80045

720-859-4060

fax 720-859-4110

www.ihctech.net 

www.ihcrg.org

 

  _  

From: Kim Donadio [mailto:one_angel_sec...@yahoo.com] 
Sent: Tuesday, May 29, 2012 12:59 PM
To: pru...@ihctech.net; Dorothy Ragland-Glass; David Kemler; Fellow HistoNetters
Subject: Re: [Histonet] Unregistered techs

 

Lets just get to the crux of all this shall we. 

 

In some states where license rules. It goes like this. 

 

I'll use Florida because thats where I am. 

 

Back in the day the state offered their own test. 3 of them to be specific, 
technician, technologist and general lab. 

 

The state decided to stop giving test so they went and allowed the ASCP to 
offer the way to becoming a histotech. 

 

Now dont get me wrong, I have a great deal of respect for ASCP, although I do 
feel they lowered the standards when they got rid of the technical test 
portion. 

 

I know many histotechs that went through a internet AS degreed program, such as 
Florida State College. a Great program by the way. They still require students 
to go to campus occasionally and a long technical practicum somehwere. These 
students get QUALITATIVE CHEMISTRY and microbiology. plus a very intensive 
practicum. You used to have to take immunology, hematology, phlebotomy and some 
other stuff I cant remember. Never the less still one of the best colleges to 
seek good techs from. < yes, Im biased. 

 

But here's what I'm seeing. Many of these out of state programs take in 
students they NEVER lay an eye on. These people pass a test and they are 
histotechs. And guess what, many of these students all they care about is 
passing that test and getting that title and fat paycheck much like all I care 
about taxes is that Ive filled it out and now send me a return (hopefully). 

 

I'm going to catch a bunch of stuff for this. But if none of you want to 
address it, then I have no choice. Because I'm just stuborn that way and I 
really care about this profession and by god Im getting older and desire for 
people coming into this profession to have enough knowledge to solve problems. 
Why? because patient care really is still driving me after all these years. 

 

So back to license or requiring ASCP. No, you dont have to have ASCP if youve 
went though all this other stuff Ive talked about in Florida. You are lisensed. 
But if you are new, yes you have to get ASCP to get one. 

 

But even if you go through a online internet program and DO NOT have a higher 
education, Florida will still only reconize to license you as a technician. You 
will need to have some years under your belt as a technician to take the route 
to become technologist, then more years to become supervisor. 

 

There is no real OJT in Florida, you would have to be registered with the state 
and given a temporary license and you would have to show where you are studying 
in a approved course to get it. 

 

Who knows, maybe you even had your husband do the program for you while you 
were at work or making dinner. But you still have to pass that test so maybe 
you are a good test taker. Who knows. 

 

Now, if anyone wants to bash me up, go ahead. Because I have had at least 8 
people in the last 2 years who couldnt even tell me what stain stains a nucleus 
who has graduated from a online course. 

 

If you want respect, raise the bar. If you raise it so high that I have even 
half to jump through more hoops , so be it. But dont just ignore where we ALL 
know the problem is. We are NOT politicians. 

 

I care for this place and all of you as professionals and I am sorry for being 
so blunt because I know this is going to hurt me. 

 

 

Good Day. 

 

Kim D

 

 


 

From: "pru...@ihctech.net" 
To: Dorothy Ragland-Glass ; David Kemler 
; Fellow HistoNetters  
Sent: Tuesday, May 29, 2012 1:18 PM
Subject: RE: [Histonet] Unregistered techs



  There is nothing volunteer about being ASCP certified as an HT or= HTL
  where  I have worked for the last 35 years, all those employed as HT's
  =  at  the  U of Colorado must be ASCP certified and I believe this is
  the case = for most other places doing hospital based Histology, work,
  right

  





  
--= -- Original Message 
  
Subject: Re: [Histonet] Unregistered tec= hs
  
From: Dorothy Ragland-Glass <[1]techman...@yahoo.com>
  

RE: [Histonet] Thank you

[Patsy Ruegg]

The best markers I have ever used are called KP Markers, they were off the
market for a while, but they are back and we get them from Mercedes Medical,
just got a new batch and they are wonderful just like the old KP markers, we
won't have anything else in the lab.

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock
Sent: Wednesday, April 11, 2012 2:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Thank you

Hi Everyone:

I want to thank everyone who gave me advice concerning my cassette 
labeling problem.
I am trying out three different types of markers pens: StatLab, Leica, 
and NewComer Supply Histo-tec brands.
We will also make sure to let the ink dry before putting the cassettes 
into formalin.
If we still have a problem, we will experiment with a different cassette.
Thanks again.

Tim Wheelock
Harvard Brain Bank
McLean Hospital
Belmont, MA
617-855-3592

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RE: [Histonet] Placenta Helper

[Patsy Ruegg]

Native Americans have eaten placenta and us old hippies started doing it
back in the 60's, I missed that one myself though.  I just read about a
Hollywood actress who ate her placenta, makes sense, need to replenish all
the blood and nutrients lost in giving birth, dogs do it.

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcia
Fisher
Sent: Thursday, March 29, 2012 11:34 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Placenta Helper

Placenta eaters have been around for a long time.  In fact, many years ago
on the original Saturday Night Live, Jane Curtin and Laraine Newman did an
absolutely hilarious skit on Placenta Helper (Hamburger Helper).  You can
probably find it online.  It's also been known that fresh placentas make
great rose bush fertilizer and catfish bait!

Marcia Fisher
Histology Supervisor/Lab Safety Officer
El Centro Regional Medical Center


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prohibited. If you are not the intended recipient, PLEASE contact the sender
and promptly destroy this e-mail and its attachments.
B 


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RE: [Histonet] Re: "placenta encapsulation"

[Patsy Ruegg]

I took my granddaughters placenta at Beaumont Hospital in MI, we just told
her OB/GYN in advance and he had a specimen jar of formalin in the delivery
room, He cut off a piece of the placenta and put it in the specimen jar for
us, handed it to me and I took it home.  We use it as our placenta control
tissue in the lab now. 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amos Brooks
Sent: Thursday, March 29, 2012 2:39 PM
To: histonet@lists.utsouthwestern.edu; cmil...@gladstone.ucsf.edu
Subject: [Histonet] Re: "placenta encapsulation"

Hi,
I would recommend getting someone else to do it for you. Unless you are
*really* resilient, I would expect you would have other recovery related
things to think about. With the OB/GYN's permission (and curiosity) I took
my wife's placenta twice. Once for each kid. The local college was grateful
to have it since it is a great tissue to learn on, and I also had an
abundance of control tissue. The shock on my mother-in-law's face was
palpable. She still thinks I'm nuts.

Congratulations & Good Luck,
Amos


On Wed, Mar 28, 2012 at 10:10 PM,  wrote:

> Message: 14
> Date: Wed, 28 Mar 2012 18:40:39 -0700
> From: Caroline Miller 
> Subject: [Histonet] Re: "placenta encapsulation"
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <8398dfb1-c338-4d6c-a63e-e76ee5df8...@gladstone.ucsf.edu>
> Content-Type: text/plain;   charset=us-ascii
>
> WellI am 6 months pregnant, and was planning on taking a pot of
> formalin and a few blades into the delivery suite to get some of my
> placenta...I am in real need of a good CD31 and CD34 control!
>
> We all give our bodies to this - right ;P
>
> Caroline
>
>
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RE: [Histonet] Lets talk forceps

[Patsy Ruegg]

I get my forceps, even the fine bent ones at Hobby Lobby in the jewelry
making section, they are much cheaper than the medical supply companies and
in my experience just as good. 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours
Sent: Friday, April 06, 2012 1:03 PM
To: Bartlett, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Lets talk forceps

Roboz makes great forceps and they aren't expensive.  I think Storz may
have bought them out.

Emily


The whole point of this country is if you want to eat garbage, balloon up
to 600 pounds and die of a heart attack at 43, you can! You are free to do
so. To me, that’s beautiful.
--Ron Swanson



On Fri, Apr 6, 2012 at 9:43 AM, Bartlett, Jeanine (CDC/OID/NCEZID) <
j...@cdc.gov> wrote:

> I really like these and I have small hands and have had carpal tunnel
> surgery.
>
>  Surgipath ergonomic forceps now available through Leica:
>
> 38DI15585
> 38DI15590
>
> These both have a 5 ½" handle.  One is curved and the other straight.
>
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu [mailto:
> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tiffeny Magee
> Sent: Friday, April 06, 2012 9:33 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Lets talk forceps
>
> I would love to buy a top of the line pair of forceps. One in partial that
> is smallish for a woman's hand and most importantly doesn't stick to the
> tissue on the water bath when I'm separating my sections. So does anyone
> have histology HT forceps they highly recommend?
>
>
>
> Thanks
>
> Tiffeny Magee
>
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RE: [Histonet] Histobath

[Patsy Ruegg]

Is Shandon still around, I never see them anymore?

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood,
Margaret
Sent: Friday, April 06, 2012 12:52 PM
To: 'marilyn.a.we...@kp.org'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Histobath

I googled Histobath and Shandon sells them, plus some other on-line
companies.  Check it out. 


Peggy Sherwood
Research Specialist, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherw...@partners.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
marilyn.a.we...@kp.org
Sent: Friday, April 06, 2012 2:40 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Histobath

We are desperately looking for a Histobath. I know they do not make them 
anymore but if someone has a old one they are not using or a company can 
get their hands on one, we would be eternally  grateful. Our Lab Manager 
would prefer we do not us Liquid Nitrogen. We love the Histobaths we have 
now. 
Marilyn Weiss HT (ASCP) cm
Kaiser Permanente Hospital
San Diego, Ca
marilyn.a.we...@kp.org

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RE: [Histonet] Re: bone implants

[Patsy Ruegg]

This is an age old problem, bone comes off easily especially during HIER,
which is why I have adapted all my bone IHC abs to use EIER instead of HIER,
usually with pepsin or proteinase K.  Also, it helps to heat the slides flat
on a slide warmer at 45dc overnight.  If all else fails I use elmer glue
coated slides but those can be problematic because the glue may react with
some ab reagents.

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pru...@ihctech.net
web site www.ihctech.net
 

This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
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any dissemination, distribution, copying, or other use of this message, or
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this e-mail as soon as possible.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Reynolds,Donna M
Sent: Wednesday, February 15, 2012 9:49 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Re: bone implants

I don't know about implants but we were having a terrible time with bone
marrows coming off. Usually they came off during the HEIR using the steamer.
At that point we were placing our slides lying flat in a 55-60º oven
overnight which usually helps tremendously with difficult tissue. This was
followed with routine deparaffinization and hydration. It was suggested to
us to try a 70º water bath for 2-4 hours for HEIR. I was doubtful that my
antibodies would work but they did. In fact the staining was better with 2
hr HEIR than with 4. 

 Donna Reynolds Chief Histology laboratory, Research
Department Cancer Biology
U.T. M.D. Anderson Cancer Center, Houston, TX.
713-792-8106

Message: 4
Date: Mon, 13 Feb 2012 09:53:15 -0600
From: "del phillips" 
Subject: [Histonet] IHC and bone implants
To: 
Message-ID: <000a01ccea67$9958da90$cc0a8fb0$@vetmed.lsu.edu>
Content-Type: text/plain;   charset="us-ascii"

Does anyone have any suggestions for staining bone implants? I am having
trouble with wash offs. I use charged slides and they are still washing off.

 

Thanks

Del

 






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