Re: [Histonet] Histonet Digest, Vol 247, Issue 4

[Izak Dimenstein via Histonet]

What about Rene J. Buesa Histology without Xyline. Annals of Diagnostic 
Pathology, 05 Feb 2009, 13(4):246-256
https://doi.org/10.1016/j.anndiagpath.2008.12.005 PMID: 19608083
Share this article Share with emailShare with twitterShare with linkedinShare 
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From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Sunday, June 9, 2024 1:00 PM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 247, Issue 4

Send Histonet mailing list submissions to
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Re: [Histonet] Histonet Digest, Vol 245, Issue 20

[Tony Henwood via Histonet]

A few years ago there was an article on the Block - "What is Denatured Alcohol 
and What are the Implications For Histopathology?" that might be useful. The 
URL is

https://www.nsh.org/blogs/tony-henwood/2020/02/11/what-is-denatured-alcohol-and-what-are-the-implica?_gl=1*1mgiiv6*_ga*MTkyMzY5MTQ4Mi4xNzExNDA1ODQ2*_ga_7J0VYE6519*MTcxNDU0NzgyNy4xMC4xLjE3MTQ1NDc4NzcuMC4xLjEzNzYzNTQ4NjQ.&_ga=2.47649445.329273616.1714547828-1923691482.1711405846



Regards,



Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired)

Principal Scientist, the Children’s Hospital at Westmead (Retired)

Adjunct Fellow, School of Medicine, University of Western Sydney.


From: John O’Brien via Histonet 
Sent: 01 May 2024 01:49
To: histonet@lists.utsouthwestern.edu 
Subject: Re: [Histonet] Histonet Digest, Vol 245, Issue 20

Ethyl Alcohol,
Reagent 100% alcohol is what is normally used in Pathology processing and 
slide staining
IMEB Inc offer all grades of Reagent alcohol, pure ethyl is a controlled 
alcohol regulated and taxed by government
Regards
John

> On Apr 30, 2024, at 10:18 AM, histonet-requ...@lists.utsouthwestern.edu wrote:
>
> Send Histonet mailing list submissions to
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> To subscribe or unsubscribe via the World Wide Web, visit
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>
> Today's Topics:
>
>   1. Alcohol (Naira Margaryan)
>
>
> --
>
> Message: 1
> Date: Mon, 29 Apr 2024 18:49:04 -0500
> From: Naira Margaryan 
> To: Histonet 
> Subject: [Histonet] Alcohol
> Message-ID:
>
> Content-Type: text/plain; charset="UTF-8"
>
> Hello,
>
> What type of ALCOHOL ETHYL you?re using in your histology lab.?
>
> We are thinking to get 20-30-gal drum.
>
> Could you please help me with that?
>
>
>
> Your suggestion is appreciated,
>
> Naira
>
>
> --
>
> Subject: Digest Footer
>
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> --
>
> End of Histonet Digest, Vol 245, Issue 20
> *****


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Re: [Histonet] Histonet Digest, Vol 245, Issue 20

[John O’Brien via Histonet]

Ethyl Alcohol,
Reagent 100% alcohol is what is normally used in Pathology processing and 
slide staining
IMEB Inc offer all grades of Reagent alcohol, pure ethyl is a controlled 
alcohol regulated and taxed by government 
Regards
John

> On Apr 30, 2024, at 10:18 AM, histonet-requ...@lists.utsouthwestern.edu wrote:
> 
> Send Histonet mailing list submissions to
>histonet@lists.utsouthwestern.edu
> 
> To subscribe or unsubscribe via the World Wide Web, visit
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> 
> You can reach the person managing the list at
>histonet-ow...@lists.utsouthwestern.edu
> 
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> 
> 
> Today's Topics:
> 
>   1. Alcohol (Naira Margaryan)
> 
> 
> --
> 
> Message: 1
> Date: Mon, 29 Apr 2024 18:49:04 -0500
> From: Naira Margaryan 
> To: Histonet 
> Subject: [Histonet] Alcohol
> Message-ID:
>
> Content-Type: text/plain; charset="UTF-8"
> 
> Hello,
> 
> What type of ALCOHOL ETHYL you?re using in your histology lab.?
> 
> We are thinking to get 20-30-gal drum.
> 
> Could you please help me with that?
> 
> 
> 
> Your suggestion is appreciated,
> 
> Naira
> 
> 
> --
> 
> Subject: Digest Footer
> 
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> 
> --
> 
> End of Histonet Digest, Vol 245, Issue 20
> *


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Re: [Histonet] Histonet Digest, Vol 245, Issue 17

[Bob Richmond via Histonet]

Ann Specian (where?) asks about Epredia Xylene Substitute:


>>We are thinking of changing from xylene to this substitute. Does anybody
have any Processing protocols using Epredia Xylene?substitute that they
could share?<<


Well, what’s in it? You’re asking your readers to look it up for you.


I Googled it, and found the SDS (materials safety data sheet) buried many
pages down in the Epredia web site:


https://epredia.webdamdb.com/bp/#/folder/7477836/104813679


It’s identified only as “naphtha”. That’s a petroleum cut that’s heavier
than gasoline, more like kerosene. Dry-cleaners used it before they went to
less flammable alternatives.


So it’s an “aliphatic” clearing agent, but a very crude one. If you want an
aliphatic (rather than a turpentine-like lemon-smelling preparation), I’d
find one specially formulated for tissue processing, rather than this
repurposed cleaning fluid.


Bob Richmond

Maryville, TN

On Thu, Apr 25, 2024 at 1:14 PM 
wrote:

> Send Histonet mailing list submissions to
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>
> To subscribe or unsubscribe via the World Wide Web, visit
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>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
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>
> Today's Topics:
>
>1. Ya Know That ONE Place YOU always wanted to LIVE? Is it   on
>   this LIST? (rel...@earthlink.net)
>2. Epredia xylene substitute (Ann Specian)
>
>
> --
>
> Message: 1
> Date: Wed, 24 Apr 2024 13:03:37 -0400
> From: 
> To: 
> Subject: [Histonet] Ya Know That ONE Place YOU always wanted to LIVE?
> Is it   on this LIST?
> Message-ID: <000901da9669$5a478520$0ed68f60$@earthlink.net>
> Content-Type: text/plain;   charset="us-ascii"
>
> Hi Histonetters!
>
> Happy Wednesday!  Yeah it's hump day but
>
> Wednesday is my favorite day of the week because I get most of
>
> my greatest opportunities!
>
> I am seeing a trend.
>
> I am hearing from clients in places that have VERY LOW TURNOVER!
>
> I KNOW there are histopeeps out there JUST WAITING for the RIGHT
> Opportunity
> in
>
> The PERFECT LOCATION!
>
> I am here to tell you it can happen anytime!
>
> Here are some examples of the areas I am working on
>
> RIGHT NOW!
>
> Montana
>
> Arizona
>
> South Dakota
>
> Oregon
>
> Indiana
>
> Virginia
>
> Tennesee
>
> Alabama
>
> Georgia
>
> Florida
>
> And new opportunities coming in all of the time!
>
> Is there a location I should add to my list?
>
> These are permanent full time positions and my clients offer excellent
> compensation, relo/sign on bonuses up to 20K
>
> and a fantastic team to work with!
>
> If you would like more info...
>
> Contact ME!
>
> email: rel...@earthlink.net 
>
> cell/text 407-353-5070
>
>
>
> Thanks-Pam
>
>
>
> Right Time, Right Place, Right Move with RELIA!
>
> Providing excellent service exclusively to the Histology Community!
>
>
>
> Thank You!
>  Pam M. Barker
>
> Pam Barker
> President/Senior Recruiting Specialist-Histology
> RELIA Solutions
> Specialists in Allied Healthcare Recruiting
> 5717 Red Bug Lake Road #330
> Winter Springs, FL 32708-4969
> Phone: (407)657-2027
> Cell:   (407)353-5070
> FAX:   (407)678-2788
>
> Toll free: (866)60RELIA or (866)607-3542
> E-mail:   rel...@earthlink.net
>
>  
> https://www.facebook.com/RELIASolutionsforhistologyprofessionals
>
>  
> www.linkedin.com/in/reliasolutions
>
> #ilovemyhistopeeps
>
> #jobs4myhistopeeps
>
> #histologyiscool
>
> #histologyjobs
>
> #histologycareers
>
> #histology
>
>
>
>
>
>
>
> --
>
> Message: 2
> Date: Wed, 24 Apr 2024 18:59:37 + (UTC)
> From: Ann Specian 
> To: 
> Subject: [Histonet] Epredia xylene substitute
> Message-ID: <1779091641.2783754.1713985177...@mail.yahoo.com>
> Content-Type: text/plain; charset=UTF-8
>
> We are thinking of changing from xylene to this substitute. Does anybody
> have any Processing protocols using Epredia Xylene?substitute that they
> could share?
>
>
> Sent from the all new AOL app for iOS
>
>
> --
>
> Subject: Digest Footer
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> --
>
> End of Histonet Digest, Vol 245, Issue 17
> *
>
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Re: [Histonet] Histonet Digest, Vol 245, Issue 17

[John O’Brien via Histonet]

Very slow drying of all substitutes 

> On Apr 25, 2024, at 10:11 AM, histonet-requ...@lists.utsouthwestern.edu wrote:
> 
> Send Histonet mailing list submissions to
>histonet@lists.utsouthwestern.edu
> 
> To subscribe or unsubscribe via the World Wide Web, visit
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
>histonet-requ...@lists.utsouthwestern.edu
> 
> You can reach the person managing the list at
>histonet-ow...@lists.utsouthwestern.edu
> 
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> 
> 
> Today's Topics:
> 
>   1. Ya Know That ONE Place YOU always wanted to LIVE? Is iton
>  this LIST? (rel...@earthlink.net)
>   2. Epredia xylene substitute (Ann Specian)
> 
> 
> --
> 
> Message: 1
> Date: Wed, 24 Apr 2024 13:03:37 -0400
> From: 
> To: 
> Subject: [Histonet] Ya Know That ONE Place YOU always wanted to LIVE?
>Is iton this LIST?
> Message-ID: <000901da9669$5a478520$0ed68f60$@earthlink.net>
> Content-Type: text/plain;charset="us-ascii"
> 
> Hi Histonetters!
> 
> Happy Wednesday!  Yeah it's hump day but
> 
> Wednesday is my favorite day of the week because I get most of
> 
> my greatest opportunities!
> 
> I am seeing a trend.
> 
> I am hearing from clients in places that have VERY LOW TURNOVER!
> 
> I KNOW there are histopeeps out there JUST WAITING for the RIGHT Opportunity
> in
> 
> The PERFECT LOCATION!
> 
> I am here to tell you it can happen anytime!
> 
> Here are some examples of the areas I am working on
> 
> RIGHT NOW!
> 
> Montana
> 
> Arizona
> 
> South Dakota
> 
> Oregon
> 
> Indiana
> 
> Virginia
> 
> Tennesee
> 
> Alabama
> 
> Georgia
> 
> Florida
> 
> And new opportunities coming in all of the time!
> 
> Is there a location I should add to my list?
> 
> These are permanent full time positions and my clients offer excellent
> compensation, relo/sign on bonuses up to 20K
> 
> and a fantastic team to work with!
> 
> If you would like more info...
> 
> Contact ME!
> 
> email: rel...@earthlink.net 
> 
> cell/text 407-353-5070
> 
> 
> 
> Thanks-Pam
> 
> 
> 
> Right Time, Right Place, Right Move with RELIA!
> 
> Providing excellent service exclusively to the Histology Community!
> 
> 
> 
> Thank You!
> Pam M. Barker
> 
> Pam Barker
> President/Senior Recruiting Specialist-Histology
> RELIA Solutions
> Specialists in Allied Healthcare Recruiting
> 5717 Red Bug Lake Road #330
> Winter Springs, FL 32708-4969
> Phone: (407)657-2027
> Cell:   (407)353-5070
> FAX:   (407)678-2788
> 
> Toll free: (866)60RELIA or (866)607-3542
> E-mail:   rel...@earthlink.net   
> 
> 
> https://www.facebook.com/RELIASolutionsforhistologyprofessionals
> 
> 
> www.linkedin.com/in/reliasolutions
> 
> #ilovemyhistopeeps
> 
> #jobs4myhistopeeps
> 
> #histologyiscool
> 
> #histologyjobs
> 
> #histologycareers
> 
> #histology
> 
> 
> 
> 
> 
> 
> 
> --
> 
> Message: 2
> Date: Wed, 24 Apr 2024 18:59:37 + (UTC)
> From: Ann Specian 
> To: 
> Subject: [Histonet] Epredia xylene substitute
> Message-ID: <1779091641.2783754.1713985177...@mail.yahoo.com>
> Content-Type: text/plain; charset=UTF-8
> 
> We are thinking of changing from xylene to this substitute. Does anybody have 
> any Processing protocols using Epredia Xylene?substitute that they could 
> share?
> 
> 
> Sent from the all new AOL app for iOS
> 
> 
> --
> 
> Subject: Digest Footer
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> --
> 
> End of Histonet Digest, Vol 245, Issue 17
> *


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Re: [Histonet] Histonet Digest, Vol 245, Issue 9

[dogsloveus2 via Histonet]

Hi Pam!
Just curious about the position in Salem, Virginia.  Is it a permanent 
position?  Do you have details on it?  
Thanks!  Tara

Yahoo Mail: Search, Organize, Conquer 
 
  On Fri, Apr 12, 2024 at 12:11 PM, 
histonet-requ...@lists.utsouthwestern.edu
 wrote:   Send Histonet mailing list submissions to
    histonet@lists.utsouthwestern.edu

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When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

  1. ICYMI: YES! You Should Ask For A Raise Via Email and    Here's
      HOW! Plus A "Plan B" (rel...@earthlink.net)


--

Message: 1
Date: Thu, 11 Apr 2024 13:18:42 -0400
From: 
To: 
Subject: [Histonet] ICYMI: YES! You Should Ask For A Raise Via Email
    and    Here's HOW! Plus A "Plan B"
Message-ID: <024101da8c34$4ea6c030$ebf44090$@earthlink.net>
Content-Type: text/plain;    charset="us-ascii"

Hey Histonetters!

I hope you are having a great day!

I have been hearing from a lot of your fellow histopeeps asking for
strategies on how to ask for a raise.

I saw this great article - I could not have written it better myself LOL!

So why reinvent the wheel?

ICYMI: How to Ask for a Rais VIA Email - a Step by Step Guide!  And a few
Interesting Opportunities!

https://www.themuse.com/advice/how-to-ask-for-a-raise-via-email

If you are unsuccessful in your quest for a raise...

OR

Are Interested in a New Opportunity...

Here Are The Exciting Opportunities that I am currently working on.

Leadership and Specialty positions:

IHC Tech          CA - S.F. Bay Area

Mohs Tech        AZ - Tucson

Histo Supervisor  SD - Rapid City

Histo Supervisor  GA - Atlanta

Here are the Tech Positions:

HistoTech        VA - Salem

Histotech        VA - Virginia Beach

Histotech        AL - Birmingham

Histotech        FL - Ft. Myers

IHC Tech          VA - Virginia Beach

Mohs Tech        AZ - Tucson

Histotech        TN - Chattanooga

Histotech        VA - Charlottesville

You can Use your histology skills to contribute, grow and learn!

My clients are in need of amazing histotechs Like YOU!!

My clients are offering exciting opportunities that are permanent full-time
positions 

with excellent compensation and benefits, and relocation/sign on bonuses up
to 20K!

You can FINISH your Travel Assignment!

You can TAKE your vacation time!

Would you be interested in more information on any these positions?

OR

Do you know of anyone who might be interested?

I really appreciate you taking the time to read this e-mail and it means a
lot to me when you take the time to refer your friends and coworkers so to
show you my appreciation, I would like to offer you a 250.00 referral fee
for anyone you refer to me that I place.

 

If you or someone you know might be interested, please contact me. 

I can be reached:

toll free at 866-607-3542

cell/text407-353-5070

email me at rel...@earthlink.net  

If you are contemplating a move but NOT to one of these areas, please let me
know.  

I am getting calls for histology professionals at all levels  practically
everywhere on  almost DAILY basis!!

 

Join your fellow histopeeps for fun and information at my Facebook group
just for you!!

 

www.facebook.com/groups/histotechnologists
 

 

I just need 25 more members to get to 5K!!!

 

Right Time, Right Place, Right Move with RELIA!

Providing excellent service exclusively to the Histology Community!

 

Thanks-Pam

 

Right Time, Right Place, Right Move with RELIA!

Providing excellent service exclusively to the Histology Community!

 

Thank You!
 Pam M. Barker 

Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5717 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:      (407)353-5070
FAX:      (407)678-2788

Toll free: (866)60RELIA or (866)607-3542
E-mail:   rel...@earthlink.net  

 
https://www.facebook.com/RELIASolutionsforhistologyprofessionals 

 
www.linkedin.com/in/reliasolutions 

Follow my hashtags to make your day great and your career greater!

#ilovemyhistopeeps

#jobs4myhistopeeps

#histologyiscool

#histologyjobs

#histologycareers

#histology

 

 



--

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Re: [Histonet] Histonet Digest, Vol 243, Issue 7

[Bob Richmond via Histonet]

>
> Re: [Histonet] Modified Davidson
>
> The formula for "modified Davidson's fixative" that I used was three parts
tap water, three parts reagent alcohol, two parts 37% formaldehyde ("strong
formalin"), one part glacial acetic acid. Best mixed under a fume hood.

At 85 I must be one of the last left standing with much experience with it.
I learned it when I was a resident at Johns Hopkins around 1970, when
surgical pathologist Bill Hartmann introduced it at the request of medical
geneticist Victor McCusick, who wanted it for fixing skin biopsy specimens
to look for nuclear sex chromatin bodies ("Barr bodies") as specified by
Moore & Barr in their 1950s publications on this important discovery.

The fixative got quite popular with the GYN pathologists, who ran an
entirely separate service, replacing their old "Vandegrift's fixative" for
all their specimens. From there it seems to have spread to other pathology
services, eventually becoming various proprietary fixatives such as "O-Fix".

You could always identify it by the characteristic "airplane dope" (for
boys)" or "nail polish remover" (for girls) aroma, as ethyl acetate
accumulated in the aging fixative as the alcohol and acetic acid slowly
esterified. The "modified" referred to the omission of glycerol from what
was supposed to be Davidson's original formula.

John Kiernan some years ago noted on Histonet that the formula wasn't
entirely rational, and was very tolerant of various modifications. He said
then that when he retired he'd try to find Davidson's original source among
Davidson's unpublished papers.

I think Davidson's fixative became largely obsolete for two reasons. Better
embedding waxes gave much improved nuclear detail over plain old paraffin
wax (what I remember long ago). And it isn't compatible with
immunohistochemical techniques.

Bob Richmond
Maryville, Tennessee
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Re: [Histonet] Histonet Digest, Vol 243, Issue 6

[Paula Keene Pierce via Histonet]

I6Mn0%3D%7C0%7C%7C%7C&sdata=eiD1an%2F2n73QFobrsXKdiC2Kk8h6AE78xAtW9DIRiAM%3D&reserved=0<http://lists.utsouthwestern.edu/mailman/listinfo/histonet>
> 
> 
> --
> 
> Message: 3
> Date: Thu, 8 Feb 2024 17:19:28 +
> From: "Stephanie L. Thompson" 
> To: "Histonet@lists.utsouthwestern.edu"
>    
> Subject: [Histonet] Lead Histotechnologist
> Message-ID: 
> Content-Type: text/plain; charset="us-ascii"
> 
> Looking for the next step in your career?
> 
> Sonic Healthcare USA has an opportunity for a Lead Histotechnologist.
> 
> Quality is in our DNA -- is it in yours?
> 
> Join our front line of #HealthcareHeroes! Our mission is to advance the 
> health and wellbeing of our communities as a leader in clinical laboratory 
> solutions.
> 
> Location: Exeter, NH
> Days: Monday - Friday
> 9:00 PM - 5:30 AM
> Full-time: Benefit Eligible
> 
> In this role, you will:
> 
> Prepare histologic slides from human tissue sections for microscopic 
> examination and diagnosis by Pathologist.
> 
>  *  Exercises independent judgment in dealing with procedural and technical 
>problems.
>  *  Examines slides and/or blocks to ensure tissue preparation is meeting 
>laboratory requirements.
>  *  Trains or directs Laboratory Assistants and Histology Technicians engaged 
>in laboratory testing and processing techniques.
>  *  Prepares sections of human tissue for microscopic examination and patient 
>diagnosis, using techniques to gross (dissect tissue), embed (orient specimen 
>in paraffin block), section (cut thin sections of tissue), stain (enhance 
>contrast of tissue and highlight specific features of interest with routine 
>hematoxylin and eosin stains), and mount tissue (adhere tissue onto glass 
>slides), from surgical procedures.
>  *  Performs recuts and additional stains including special and 
>immunohistochemistry stains, as requested by a Pathologist.
>  *  Operates computerized laboratory equipment to fix, dehydrate, and 
>infiltrate with wax, tissue specimens to be preserved for study by Pathologist.
>  *  May label requisitions, specimen containers, cassettes and/or slides and 
>affixes coverslip to slides.
>  *  Maintains laboratory equipment and tracks all routine maintenance and 
>quality controls performed.
>  *  Files, retrieves, and distributes blocks, slides, and pathology reports.
>  *  Operates, cleans, and sterilizes laboratory equipment, glassware, 
>instruments, and workstation.
>  *  Disposes of hazardous chemical waste per regulatory guidelines.
>  *  Maintains strictest confidentiality.
>  *  Complies with all State, Federal, professional regulations as well as 
>company and departmental rules, polices, and procedural manuals.
>  *  Adherence to CAP, CLIA, State Regulations, HIPAA, Safety and OSHA 
>Regulations.
> 
> All you need is:
> 
>  *  High School diploma or equivalent required. Associates or Bachelors of 
>Science degree and completion of histotechnology program required. 
>Certification as a histotechnician (HT) or histotechnologist (HTL) by American 
>Society of Clinical Pathology (ASCP) preferred.
>  *  State licensure, if applicable.
>  *  Certified or eligible for Board of Certification (BOC) by the American 
>Society of Clinical Pathologists (ASCP)
>  *  Completion of a Histology program accredited by the National Accrediting 
>Agency for Clinical Laboratory Sciences (NAACLS) or minimum of one (1) year 
>experience as a full-time Histology Technician Trainee and competent in the 
>areas of fixation, processing, embedding, microtomy, grossing, special stains, 
>immunohistochemistry, and lab operations.
> 
> Company:
> Sonic Anatomic Pathology
> 
> Please feel free to contact me:
> Stephanie Thompson - 210-428-1646
> 
> 
> 
> This message contains privileged and confidential information intended only 
> for the use of the addressee named above. If you are not the intended 
> recipient of this message you must not disseminate, copy, or take any action 
> in reliance on it.
> 
> 
> --
> 
> Subject: Digest Footer
> 
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> 
> --
> 
> End of Histonet Digest, Vol 243, Issue 6
> 


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Re: [Histonet] Histonet Digest, Vol 243, Issue 6

[KATHLEEN FERNANDEZ via Histonet]

Paula,
We are using the KP’s at our lab too and have been noticing that they are 
smearing! We are having issues on our slides, since we hand write still. This 
just recently started happening. Maybe they changed their formula?

Kathy
Sent from my iPhone

> On Feb 8, 2024, at 12:01 PM, histonet-requ...@lists.utsouthwestern.edu wrote:
> 
> Send Histonet mailing list submissions to
>histonet@lists.utsouthwestern.edu
> 
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>histonet-ow...@lists.utsouthwestern.edu
> 
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> 
> 
> Today's Topics:
> 
>   1. Marking Pen (Paula)
>   2. Re: Marking Pen (Piche, Jessica)
>   3. Lead Histotechnologist (Stephanie L. Thompson)
> 
> 
> --
> 
> Message: 1
> Date: Wed, 7 Feb 2024 11:35:26 -0800
> From: "Paula" 
> To: 
> Subject: [Histonet] Marking Pen
> Message-ID: <009e01da59fc$ce13b680$6a3b2380$@biopath.org>
> Content-Type: text/plain;charset="us-ascii"
> 
> Hello,
> 
> 
> 
> We have been using KP Marker Plus pens for cassettes.  We have 2 Leica
> Processors and 1 VIP6 processor.  The cassettes that go into the VIP6 are
> smeared and some are almost smeared off completely.  The processors have the
> same solutions in them.
> 
> 
> 
> If anyone can shed some light as to why there is a difference, and if anyone
> can recommend a better marking pen for us to try, I would appreciate the
> feedback.
> 
> 
> 
> Thank you,
> 
> Paula
> 
> 
> 
> --
> 
> Message: 2
> Date: Thu, 8 Feb 2024 11:30:03 +
> From: "Piche, Jessica" 
> To: "histonet@lists.utsouthwestern.edu"
>,Paula 
> Subject: Re: [Histonet] Marking Pen
> Message-ID:
>
> 
>
> Content-Type: text/plain; charset="us-ascii"
> 
> Hi Paula,
> 
> What is the first solution the cassettes go in when the processor starts? We 
> use Statmark pens when our cassette printer isn't working, and they work 
> well. The only time we have had issues was when we would hand write the 
> cassettes and run them on our small biopsy run which skips the formalin. It 
> seems like the marker ink needs to "fix" with the formalin. Sometimes they 
> smear if they aren't dry enough before they go into formalin too. I hope you 
> figure it out. Maybe see if you can get some samples of different pens and 
> then run some empty cassettes and see what works best for you.
> 
> Have a good day.
> 
> Jessica
> 
> Jessica Piche, HT(ASCP)
> Waterbury Hospital Histology Laboratory
> Histology Team Leader
> 203-573-7167
> 
> From: Paula via Histonet 
> Sent: Wednesday, February 7, 2024 2:35 PM
> To: histonet@lists.utsouthwestern.edu 
> Subject: [Histonet] Marking Pen
> 
> [EXTERNAL MSG]
> 
> Hello,
> 
> 
> 
> We have been using KP Marker Plus pens for cassettes.  We have 2 Leica
> Processors and 1 VIP6 processor.  The cassettes that go into the VIP6 are
> smeared and some are almost smeared off completely.  The processors have the
> same solutions in them.
> 
> 
> 
> If anyone can shed some light as to why there is a difference, and if anyone
> can recommend a better marking pen for us to try, I would appreciate the
> feedback.
> 
> 
> 
> Thank you,
> 
> Paula
> 
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> 
> 
> --
> 
> Message: 3
> Date: Thu, 8 Feb 2024 17:19:28 +
> From: "Stephanie L. Thompson" 
> To: "Histonet@lists.utsouthwestern.edu"
>
> Subject: [Histonet] Lead Histotechnologist
> Message-ID: 
> Content-Type: text/plain; charset="us-ascii"
> 
> Looking for the next step in your career?
> 
> Sonic Healthcare USA has an opportunity for a Lead Histotechnologist.
> 
> Quality is in our DNA -- is it in yours?
> 
> Join our front line of #HealthcareHeroes! Our mission is to advance the 
> health and wellbeing of our communities as a leader in clinical laboratory 
> solutions.
> 
> Location: Exeter, NH
> Days: Monday - Friday
> 9:00 PM - 5:30 AM
> Full-time: Benefit Eligible
> 
> In this role, you will:
> 
> Prepare histologic slides from human tissue sections for microscopic 
> exami

Re: [Histonet] Histonet Digest, Vol 242, Issue 10

[Izak Dimenstein via Histonet]

I would like to add a couple cents to Brian's suggestions, especially how  to 
embed the gross section. The "theory" in presented in my book Izak Dimenstein 
Grossing Bones: Principles, Technique, and Instruments. It depends on the level 
of hardness of the bone after decalcification or in the case of undemineralized 
bone section.

Izak

From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Thursday, January 25, 2024 1:00 PM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 242, Issue 10

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Re: [Histonet] Histonet Digest, Vol 237, Issue 4

[Eddie Martin via Histonet]

gt;
>
>
>
>
> -- Forwarded message --
> From: Betsy Molinari 
> To: Histonet 
> Cc:
> Bcc:
> Date: Thu, 10 Aug 2023 14:57:31 +
> Subject: [Histonet] Sudan Black B
> Hi,
> I have been asked to do a Sudan stain on a heart  biopsy for lipofuscin.
> The biopsy is in a paraffin block. They are looking  to better report and
> understand the IHC. I am totally unfamiliar with this stain. I did some
> reading but have been unable to find a protocol for paraffin sections. I
> found a reference to Sheehan & Hrapchak (1973) but unfortunately I don't
> have that edition.  Any ideas would be greatly appreciated .
>
> Betsy Molinari HT (ASCP)
> Texas Heart Institute
> Cardiovascular Pathology
> 1101 Bates St.
> Houston, Texas  77030
> 832-355-6524
>
> Betsy Molinari, HT (ASCP)
> Sr. Histology Research Technician
> CV Pathology Research
>
> The Texas Heart Institute (r)
> 6770 Bertner Avenue, MC 1-283
> Houston, TX 77030
>
> Office: 832-355-6524 | Fax: 832-355-6812
> Email: bmolin...@texasheart.org
> texasheart.org<https://www.texasheart.org/> | texasheartmedical.org<
> https://www.texasheartmedical.org/> | facebook<
> https://www.facebook.com/Texas.Heart.Institute> | twitter<
> https://twitter.com/Texas_Heart>
>
> CONFIDENTIALITY NOTICE: This email and attachments contain information
> that may be confidential or privileged. If you are not the intended
> recipient, notify the sender at once and delete this message completely
> from your information system. Further use, disclosure, or copying of
> information contained in this email is not authorized, and any such action
> should not be construed as a waiver of privilege or other confidentiality
> protections.
>
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Re: [Histonet] Histonet Digest, Vol 235, Issue 23

[Pat Patterson via Histonet]

Get Outlook for Android

From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Wednesday, June 28, 2023 12:00:02 PM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 235, Issue 23

[CAUTION - EXTERNAL EMAIL: Do not click any links or open any attachments 
unless you trust the sender and know the content is safe.]

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When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. Just in time for July 4th I asked ChatGPT to create   Fireworks
  using histological stains. Please enjoy and feel  free to share!
  (rel...@earthlink.net)


--

Message: 1
Date: Wed, 28 Jun 2023 12:57:27 -0400
From: 
To: 
Subject: [Histonet] Just in time for July 4th I asked ChatGPT to
create  Fireworks using histological stains. Please enjoy and feel  
free
to share!
Message-ID: <00a101d9a9e1$9f9aac30$ded00490$@earthlink.net>
Content-Type: text/plain;   charset="utf-8"



Hi Histopeeps

I hope you are having a fantastic day.
Can you believe it?s 4th of July next week?

If you have been reading my emails then you know I have been having some
fun using ChatGPT to create images.

In honor of Independence Day Here is a link to an image of Fireworks
created by ChatGPT using histological stains:



https://nam12.safelinks.protection.outlook.com/?url=https%3A%2F%2Freliasolutionspambarker.wordpress.com%2F2023%2F06%2F28%2Fjust-in-time-for-ju&data=05%7C01%7Cpat.patterson%40propath.com%7C9b575aad84c44847c4cf08db77fb4ad7%7Ceab7e4b5d8f8463b8a4ac63f87390803%7C1%7C0%7C638235693405624313%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=n%2F2YonZJXXZ%2F0r6g8EBS3mwP8xCSJcDoCw9nd64VOdA%3D&reserved=0
ly-4th-i-asked-chatgpt-to-create-fireworks-using-histological-stains-please-
feel-free-to-enjoy-and-share/(opens in a new tab)

Histopeeps, What do you think?

It?s a holiday week so I will keep the job info short and sweet.
Take a look and see if you or anyone you know might be interested in any of
these opportunities.

*If something looks interesting let me know.

*If you know someone who might be interested, please pass along the
information.

Remember if I place someone you refer to me you will earn a referral fee.

If the opportunity for you is someplace else drop me an email to remind me
where you want to be and what you want to do.

I want YOU to be the person that pops in MY head when that client calls!!!

Here are my Hottest!! Histology Opportunities:

RELIA Histology Opportunities:
I am recruiting for these positions:
*   AP Manager
*   Quality Assurance Specialist
*   Tech Support Specialist
*   Histology Manager
*   Lead Histotech
*   Histotechnicians/Histotechnologists
*   Pathologist?s Assistant

Locations are nationwide including:

Florida, California, Tennessee, Georgia, Wisconsin, New York, Arizona,
Washington & South Carolina.

I have brand new labs, hospitals, derm, gi, research private labs and new
start ups.

All of my clients offer excellent compensation, benefits and some offer
relocation assistance and or sign-on bonuses.

All of these jobs are full-time & permanent & some are

 RELIA Exclusives!!!

If you or anyone you know is interested in hearing more about any of

Re: [Histonet] Histonet Digest, Vol 235, Issue 13

[Brent Barrett via Histonet]

Unsubscribe

From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Saturday, June 17, 2023 1:00:02 PM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 235, Issue 13

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When replying, please edit your Subject line so it is more specific
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Today's Topics:

   1. Re: Losing sections (Terri  Braud)


--

Message: 1
Date: Fri, 16 Jun 2023 17:56:02 +
From: "Terri  Braud" 
To: "histonet@lists.utsouthwestern.edu"

Subject: Re: [Histonet] Losing sections
Message-ID: 
Content-Type: text/plain; charset="us-ascii"

I may have an answer for you.  Your Prisma stainer only runs the heater when 
needed.  With full runs, the oven stays warm, but later stain runs allow for 
the heater to cool down.  When your rack goes into the dry station, the heat 
comes up from the bottom to start to dry the slide, thus the bottom sections 
have enough dry time, but the top don't, and they wash off.
We used to encounter the same problem and that is what we hypothesized was 
happening, because, when we ran a blank rack through 10 minutes before loading 
the late rack, we were fine. Or, when we dried in a 60'C oven for 15 minutes 
when loading that lone late rack, we were fine.
Just an idea, but for us, no more wash offs of top sections.

Terri L. Braud, HT(ASCP)
HNL Laboratories for
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3689
Fax: 215-938-2021

Message: 4
Date: Fri, 16 Jun 2023 14:25:31 +
From: "Kolman, Kimberly D." 
Subject: [Histonet] losing sections

For your entertainment, I have a bizarre phenomenon to share with the group:
I am occasionally losing the TOP section on a deeper cut H&E slide.
Full run of daily slides do not show this issue.  A deeper slide cut later in 
the day might, or a deeper slide cut first thing in the morning may, when I've 
not run a full daily run.
I have switched to a different lot number of standard slides.  I have used 
adhesive slides. Distilled water for the water bath same as I have used for 20 
years. Using Sta-On adhesive in the water bath. H&E stain done on a Prisma Plus 
stainer, with no changes in staining procedure.  There have been NO changes to 
any of my procedures.
This is a very random happening that is boggling my mind! If any section was 
going to fall off, I'd think it would be the 2nd section - (last one picked up 
from the water bath).  I've tried to make sure the slide has thoroughly dried 
before putting it on the stainer.  Slides appear clean, and no greasy 
fingerprints on the slide.  The one I always lose is the TOP section/very first 
level taken.

Any ideas? Do I just have a Histo Gremlin here?
Thanks for your input.
Kim
Kimberly D. Kolman, HT (ASCP)
Eastern Kansas Health Care System
Eisenhower VA Medical Center - Histology 115
4101 S. 4th St. Trfwy.
Leavenworth, KS 66048
913-682-2000 x 62537
Fax: 913-758-4193



--

Subject: Digest Footer

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End of Histonet Digest, Vol 235, Issue 13
*
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Re: [Histonet] Histonet responses

[Mac Donald, Jennifer via Histonet]

Hi Anthony,
If you hit reply only you will get a response.  If you hit reply all a response 
will go you and the Histonet.
Jennifer

-Original Message-
From: Anthony Lapiana via Histonet 
Sent: Tuesday, June 13, 2023 11:58 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Histonet responses

  EXTERNAL SENDER - Exercise caution with requests, links, and attachments.

Good afternoon,

I am new to the histonet and I am trying to figure out how to reply to people's 
messages they post.

Also, do I get a notification when people reply to my responses or posts via 
email?

Thanks,
Anthony
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[Histonet] Histonet responses

[Anthony Lapiana via Histonet]

Good afternoon,

I am new to the histonet and I am trying to figure out how to reply to
people's messages they post.

Also, do I get a notification when people reply to my responses or posts
via email?

Thanks,
Anthony
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Re: [Histonet] Histonet Digest, Vol 235, Issue 1

[Janice Fuller via Histonet]

Is there fresh tissue in that part of the lab? Or anything else that would
cause it to be considered not clean?


On Fri, Jun 2, 2023 at 10:00 AM 
wrote:

> Send Histonet mailing list submissions to
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>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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> histonet-requ...@lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-ow...@lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
>1. Coffee at the desk (Hannen, Valerie)
>2. Re: Coffee at the desk (Thomas Podawiltz)
>3. Re: Coffee at the desk (McLaughlin, Stacy L.)
>4. Re: Coffee at the desk (Piche, Jessica)
>5. Get the Short Term or Long Term Lab Staffing Coverage You
>   Need ('Melissa Owens')
>
>
> --
>
> Message: 1
> Date: Thu, 1 Jun 2023 19:40:24 +
> From: "Hannen, Valerie" 
> To: "Histonet@lists.utsouthwestern.edu"
> 
> Subject: [Histonet] Coffee at the desk
> Message-ID:
> <
> dm6pr15mb3210bc2a1b2252be21fd076af6...@dm6pr15mb3210.namprd15.prod.outlook.com
> >
>
> Content-Type: text/plain; charset="us-ascii"
>
> Is there any regulation/ reason why a Histology supervisor whose desk is
> in the department and the desk area is taped off and designated as a "clean
> area (  meaning no chemicals or specimens cross the taped off area) can't
> have a closed coffee cup at their desk??  We are having a debate in our Lab
> and I wanted to get a consensus.
>
> Thanks in Advance,
>
> Valerie
>
> Valerie A. Hannen,MLT(ASCP),HTL,SU(FL)
> Histology Section Chief
> Parrish Medical Center
> 951 N. Washington Avenue
> Titusville, Florida 32796
> P: 321-268-6333  Ext. 7506
> F: 321-268-6149
> valerie.han...@parrishmed.com
> www.parrishmed.com
>
>
>
> --
>
> Message: 2
> Date: Thu, 1 Jun 2023 20:00:30 + (UTC)
> From: Thomas Podawiltz 
> To: "Histonet@lists.utsouthwestern.edu"
> ,"Hannen, Valerie"
> 
> Subject: Re: [Histonet] Coffee at the desk
> Message-ID: <162780967.2965690.1685649630...@mail.yahoo.com>
> Content-Type: text/plain; charset=UTF-8
>
>  OSHA: bloodborne pathogen standard.A clean area is an area that is closed
> off from the working environment with walls and door. Tape is not a
> appropriate alternative, maybe on WKRP in Cincinnati, but not in a lab.?
> On Thursday, June 1, 2023 at 03:40:41 PM EDT, Hannen, Valerie via
> Histonet  wrote:
>
>  Is there any regulation/ reason why a Histology supervisor whose desk is
> in the department and the desk area is taped off and designated as a "clean
> area (? meaning no chemicals or specimens cross the taped off area) can't
> have a closed coffee cup at their desk??? We are having a debate in our Lab
> and I wanted to get a consensus.
>
> Thanks in Advance,
>
> Valerie
>
> Valerie A. Hannen,MLT(ASCP),HTL,SU(FL)
> Histology Section Chief
> Parrish Medical Center
> 951 N. Washington Avenue
> Titusville, Florida 32796
> P: 321-268-6333? Ext. 7506
> F: 321-268-6149
> valerie.han...@parrishmed.com
> www.parrishmed.com
>
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>
>
> |  | Virus-free.www.avast.com |
>
>
>
> --
>
> Message: 3
> Date: Fri, 2 Jun 2023 10:09:41 +
> From: "McLaughlin, Stacy L." 
> To: "Hannen, Valerie" ,
> "Histonet@lists.utsouthwestern.edu"
> 
> Subject: Re: [Histonet] Coffee at the desk
> Message-ID:
> <
> co6pr04mb8313971f3f68e4f2822a97629b...@co6pr04mb8313.namprd04.prod.outlook.com
> >
>
> Content-Type: text/plain; charset="us-ascii"
>
> It is not allowed.  The area you want food/drink has to be completely
> separate- floor to ceiling walls and a door.
>
> Stacy McLaughlin, HT(ASCP) QLScm
> Histology Supervisor
> Cooley Dickinson Hospital
> 30 Locust Street
> Northampton, MA 01060
> Office:  (413)582-2019
> Lab:  (413)582-2179
> smclaughl...@cooleydickinson.org
>
>
>
>
> -Original Message-
> From: Hannen, Valerie via Histonet 
> Sent: Thursday, June 1, 2023 3:40 PM
> To: Histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Coffee at the desk
>
> External Email - Use Caution
>
> Is there any regulation/ reason why a Histology supervisor whose desk is
> in the department and the desk area is taped off and designated as a "clean
> area (  meaning no chemicals or specimens cross the taped off area) can't
> have a closed coffee cup at their desk??  We are having a debate in our Lab
> and I wanted to get a consensus.
>
> Thanks in A

Re: [Histonet] Histonet Digest, Vol 234, Issue 10

[Patsy Ruegg via Histonet]

Aren't they going to offend the mail histotechs with that name? Just kidding.


Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
39037 N 11th Ave
Phoenix, AZ 85086
C 720-281-5406
prueg...@hotmail.com
Doug Ruegg
C720-281-5407
douglas...@hotmail.com


From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Wednesday, May 10, 2023 11:00 AM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 234, Issue 10

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Re: [Histonet] Histonet Digest, Vol 233, Issue 7

[Patsy Ruegg via Histonet]

this is a strange post on histonet

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
39037 N 11th Ave
Phoenix, AZ 85086
C 720-281-5406
prueg...@hotmail.com
Doug Ruegg
C720-281-5407
douglas...@hotmail.com


From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Wednesday, April 19, 2023 11:00 AM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 233, Issue 7

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Re: [Histonet] Histonet Digest, Vol 233, Issue 3

[John O’Brien via Histonet]

Need pictures of microtome and blade holder to help
IMEB Inc

> On Apr 7, 2023, at 10:17 AM, histonet-requ...@lists.utsouthwestern.edu wrote:
> 
> Send Histonet mailing list submissions to
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> 
> Today's Topics:
> 
>   1. Blade holder assitance (Charles Riley)
> 
> 
> --
> 
> Message: 1
> Date: Fri, 7 Apr 2023 11:34:35 -0400
> From: Charles Riley 
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Blade holder assitance
> Message-ID:
>
> Content-Type: text/plain; charset="UTF-8"
> 
> Hello all,
> 
> I am working with a microtome that has an unusual blade holder.  Standard
> low profile blades are too short and are covered by the stage so no cutting
> occurs. The standard high profile blades stick out above the blade holder
> edge significantly and this causes wobble in the blade while sectioning
> (creating thick and thin sections and or skipping sections entirely before
> cutting a really thick section).
> 
> Can anyone suggest what type of blade I should purchase to use with this
> holder?  I was thinking maybe sapphire knife blades might fit but didn't
> want to waste money buying items I don't need
> 
> 
> --
> 
> Subject: Digest Footer
> 
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> --
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> End of Histonet Digest, Vol 233, Issue 3
> 


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Re: [Histonet] Histonet Digest, Vol 232, Issue 13

[Ewen Sutherland via Histonet]

Re Gunk

Check any of the seals or washers. When they breakdown they usually present 
with this sort of result on slides

Ewen Sutherland
Anatomical and Digital Product Specialist
Bio-Strategy
T: +61 3 9355 3900 / 1800 00 84 53
M: +61 417 460 019
ewen.sutherl...@bio-strategy.com   www.bio-strategy.com

-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 
 
Sent: Friday, March 24, 2023 4:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 232, Issue 13

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Re: [Histonet] Histonet Digest, Vol 232, Issue 7

[Eddie Martin via Histonet]

Hi kdea...@hotmail.com,

What likely is occurring but you didn’t mention in the thread is that your
friend is using alkaline phosphatase based detection, which, by itself can
react with kidney tissue. This likely explains why your friend is getting
strong kidney staining and weak melan-a staining.

This also tells me your friend didn’t optimize the antibody correctly. I’m
not sure why your friend is choosing to use a lyophilized antibody for
Melan-A as so many other commercially available options are available
capable of refrigerated storage and good up to 3 years. CellMarque makes an
Melan-A in both realty to use and/or a liquid concentrate with a 3-year
expiry date.

Best wishes,
Eddie Martin, HTL,QIHC
The National Institutes of Health
Department of Laboratory Medicine
Bone Marrow Service
eddie.mar...@nih.gov
(301)-594-2054



On Wed, Mar 15, 2023 at 1:00 PM 
wrote:

> Send Histonet mailing list submissions to
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> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> Today's Topics:
>
>1. Problems with Melan A staining (Ken M)
>
>
>
> -- Forwarded message --
> From: Ken M 
> To: "histonet@lists.utsouthwestern.edu"  >
> Cc:
> Bcc:
> Date: Wed, 15 Mar 2023 16:51:03 +
> Subject: [Histonet] Problems with Melan A staining
> One of my histotech friends is having issues with Melan A staining. Using
> MA5-27949 Melan A antibody from Thermofisher, the negative Kidney tissue
> control was stained strongly positive while the positive melanoma tissue
> control was stained weakly positive. He couldn’t figure out why after
> several tries to reduce background. Can someone give me some suggestions?
> Thank you very much!
>
>
> Ken
>
>
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Re: [Histonet] Histonet Digest, Vol 232, Issue 11

[Izak Dimenstein via Histonet]

>From my Grossing Technology book:

Gouty tissue contains monosodium urate crystals. Tissue specimens with gout 
diagnosis must be submitted in either Carnoy’s fixative (chloroform, acetic 
acid, and absolute ethanol, now almost abandoned in regular practice) or in 
absolute ethanol. Fixation cannot be in formalin, as formalin contains water 
which may dissolve any urate crystals that are present (uric acid is even more 
soluble in formalin than in water).

Once received, the specimen must be processed from 100% ethanol, skipping not 
only formalin, but also the graded alcohol series in the tissue processor. Some 
laboratories start with 70% alcohol in microwave-assisted processing. However, 
urate crystals, particularly in large deposits (tophi), often survive formalin 
fixation and routine processing anyway.

 If a gout specimen is received in formalin, it can be recovered by drying the 
fixative on a slide in an oven and mounting it unstained for Diff-Quick stain. 
Unstained slide can be viewed under polarized light. By using red filter under 
polarized light uric acid crystals can also be distinguished from calcium 
pyrophosphate dihydrate crystals (pseudogout). The rest is in the 
histotechnology realm.

Izak Dimenstein

From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Tuesday, March 21, 2023 1:00 PM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 232, Issue 11

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Re: [Histonet] Histonet Digest, Vol 231, Issue 14

[Izak Dimenstein via Histonet]

Placenta is a serious surgical pathology specimen. Wrong initial processing can 
damage the result. The described processing in the laboratory reflects complete 
misunderstanding by the management  the grossing procedure. In my book Grossing 
Technology (Amazon.com), placenta grossing technique occupies  pages 138-142.

Izak B. Dimenstein, MD, PhD, HT (ASCP)



From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Monday, February 27, 2023 1:01 PM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 231, Issue 14

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Re: [Histonet] Histonet Digest, Vol 229, Issue 10

[John O’Brien via Histonet]

IMEB Inc
Has complete catalog of all dermatology and pathology supplies and equipment 
new and refurbished for last 35 years
www.imebinc.com
IMEB Inc
Management 

> On Dec 28, 2022, at 10:08 AM, histonet-requ...@lists.utsouthwestern.edu wrote:
> 
> Send Histonet mailing list submissions to
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> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> 
> 
> Today's Topics:
> 
>   1. Dermatology Questions (ADESUPO ADESUYI)
> 
> 
> --
> 
> Message: 1
> Date: Tue, 27 Dec 2022 20:29:46 +
> From: ADESUPO ADESUYI 
> To: "histonet@lists.utsouthwestern.edu"
>
> Subject: [Histonet] Dermatology Questions
> Message-ID:
>
> 
>
> Content-Type: text/plain; charset="utf-8"
> 
> 
> Hi,
> 
> I have just started a new job at a Dermatopathology Laboratory as a 
> Laboratory Manager. Please I will appreciate it if you guys can direct me to 
> the online dermatopathology resources.
> 
> Thanks,
> 
> Adesupo Adesuyi
> 
> 
> 
> 
> 
> 
> Sent from my Metro by T-Mobile 5G Device
> 
> 
> --
> 
> Subject: Digest Footer
> 
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> 
> --
> 
> End of Histonet Digest, Vol 229, Issue 10
> *


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Re: [Histonet] Histonet Digest, Vol 228, Issue 13

[John O’Brien via Histonet]

Hi Chris
My company has large selection of refurbished and slightly  used Leica 
cryostats all models,  clinical and research
Please contact me for complete list and details ,all instruments come complete 
with full warranty 
Regards
John
IMEB Inc
www.imebinc.com

> On Nov 23, 2022, at 10:11 AM, histonet-requ...@lists.utsouthwestern.edu wrote:
> 
> Send Histonet mailing list submissions to
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> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> 
> 
> Today's Topics:
> 
>   1. Cryostat Shopping (Chris England)
> 
> 
> --
> 
> Message: 1
> Date: Wed, 23 Nov 2022 01:34:41 +
> From: Chris England 
> To: "histonet@lists.utsouthwestern.edu"
>
> Subject: [Histonet] Cryostat Shopping
> Message-ID:
>
> 
>
> Content-Type: text/plain; charset="iso-8859-1"
> 
> Aloha Histonetters--I've missed you!
> 
>  1.  It's been a very long time since I last posted here so please forgive my 
> heap of ignorance: I haven't done this in a hot minute.
>  2.  I think I'm in the market for a cryostat! But it's been 20+ years since 
> the last one I bought, and I have no clue what features I'll need now. Do I 
> buy a reliable 90s analog model? Do I need digital control? No idea! I'd love 
> to talk to sales reps to get the pitch...but because I'm most likely to buy a 
> used model, I feel like I'd be wasting their time. So I thought I'd ask you 
> fine people.
> 
> TL;DR: What features would you recommend are very useful for a cryostat for a 
> low-volume research lab? And is it a bad idea to expect to do H&E and other 
> stains on cryosectioned tissue?
> 
> To get the best advice, I'll try to anticipate some of the more likely 
> questions and answer:
> "What do you want to do?"
> Ideally, we'd like to do most of the same staining that one would do on FFPE 
> tissue (H&E and some other stains) on fixed or unfixed frozen tissue. However 
> more importantly I anticipate a lot of IHC/IF, and probably most important to 
> us is preserving lipids in sectioned tissue (hence avoiding the clearing 
> agents used with paraffin).
> "What kind of tissue?" We're a tissue engineering company working extensively 
> with mammary cells, so the workload will likely be fatty tissue, and some 
> composite samples that are tissue and polymer scaffolds (nothing a good blade 
> can't easily cut--I think).
> "What volume?" Pretty low. I may well be underestimating the workload, but 
> most likely a dozen blocks every few weeks.
> "What budget?" That what I need help with. I can probably go up to $20k, and 
> mgmt is OK with a new model if it's necessary, but frankly I wasn't fantastic 
> on a cryostat the last time I bought one so I'm not confident I'm qualified 
> to say what exactly we need.
> "What is your skill level?" Noob. I've used a cryostat before, but my 
> technique...definitely needs improvement. On the other hand, I'm the only one 
> in my company that has any experience with histology at all, so I anticipate 
> training a new generation of research histologists. So, I probably want a 
> cryostat that may not do everything for me, but also won't require a lot of 
> shoehorning.
> 
> I'll also leave a more open question down here: What is a paraffin microtome 
> better suited to than a cryostat, and vice-versa?
> 
> Thank you so, so much in advance for your time and any help you can offer!!!
> 
> Warmest regards and aloha,
> Jack England
> 
> 
> --
> 
> Subject: Digest Footer
> 
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> --
> 
> End of Histonet Digest, Vol 228, Issue 13
> *


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Re: [Histonet] Histonet Digest, Vol 228, Issue 9

[Kathy Sherwood via Histonet]

ads were very good today. Very cold out . I'll talk to you later. I'll be 
thinking of you. Much love, Kathy

From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Thursday, November 17, 2022 1:00 PM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 228, Issue 9

Send Histonet mailing list submissions to histonet@ lists. utsouthwestern. edu 
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Today's Topics:

   1. Testing for hires (Stephanie L. Thompson)
   2. Re: Testing for hires (Anne Murvosh)
   3. Re: Testing for hires (Thomas Podawiltz)


--

Message: 1
Date: Wed, 16 Nov 2022 18:18:29 +
From: "Stephanie L. Thompson" 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] Testing for hires
Message-ID: <3f3399423f9f49819059278f8e047...@sonichealthcareusa.com>
Content-Type: text/plain; charset="us-ascii"



When hiring for a histotech, how do you test their skills?

Thank you,

Stephanie
This message contains privileged and confidential information intended only for 
the use of the addressee named above. If you are not the intended recipient of 
this message you must not disseminate, copy, or take any action in reliance on 
it.


--

Message: 2
Date: Wed, 16 Nov 2022 18:24:45 +
From: Anne Murvosh 
To: "histonet@lists.utsouthwestern.edu"

Subject: Re: [Histonet] Testing for hires
Message-ID:



Content-Type: text/plain; charset="iso-8859-1"


Melt down old blocks, have them embed and cut them. Evaluate their time and 
technique. You could also do a working interview where a tech shows them around 
but has them cut and embed a few current tissues while watching. Thanks Anne

Anne Murvosh
Histology Technician

 Three Locations to Serve YOU!
   Spokane Valley   Northside   
   Coeur d'Alene
1807 N Hutchinson Rd 59 E Queen Ave, Ste 1021700 W. Riverstone Drive
Spokane, WA 99212 Spokane, WA 99207Coeur d'Alene, ID 
83814

509-456-7414  Office
509-624-0763  Fax
amurv...@advancederm.net
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-Original Message-
From: Stephanie L. Thompson via Histonet 
Sent: Wednesday, November 16, 2022 10:18 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Testing for hires



When hiring for a histotech, how do you test their skills?

Thank you,

Stephanie
This message contains privileged and confidential information intended only for 
the use of the addressee named above. If you are not the intended recipient of 
this message you must not disseminate, copy, or take any action in reliance on 
it.
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Re: [Histonet] Histonet Digest, Vol 228, Issue 3

[Katie Riley via Histonet]

Hello Cindy,

I am looking for an auto stainer and microtome. Please contact me if you have 
either.

Thank you,

Katie Riley-Hamilton H.T., M.T.QA
Technical Supervisor of Dermatopathology
Puyallup Dermatology Clinic
ka...@puyallupderm.com

2622 South Meridian
Puyallup, WA 98373

(253) 266-0799
(253) 841-2453 x. 321

Sent from Mail for Windows

From: histonet-requ...@lists.utsouthwestern.edu
Sent: Friday, November 4, 2022 10:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 228, Issue 3

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Re: [Histonet] Histonet Digest, Vol 225, Issue 14

[Eddie Martin via Histonet]

ists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet
>
> CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is
> for the sole use of the intended recipient(s) and may contain confidential
> or legally privileged information. Any unauthorized review, use, disclosure
> or distribution is prohibited. If you are not the intended recipient,
> please contact the sender by reply e-mail and destroy all copies of this
> original message.
>
>
>
>
>
>
> -- Forwarded message --
> From: Alida Bailleul 
> To: histonet 
> Cc:
> Bcc:
> Date: Wed, 31 Aug 2022 10:27:56 +0800
> Subject: [Histonet] Information on the resistance/viability of different
> tissues/cells to different freezing temperatures?
> Where can I find information on the resistance of different tissues/cells
> (e.g.,  bone, cartilage) to different freezing temperatures (e.g., -4C;
> -24C; -70C ) ? I am asking about frozen tissues that were not
> cryopreserved. I think there may be a lot of experimental studies that
> compared the resistance of cells/tissues to freeze-thaw cycles at different
> temperatures (and also compared fresh frozen versus cryopreserved) but I
> didn't really find a lot of studies. Moreover studies don't really compare
> different tissue types, they focus on one type of tissue per study.
> Please guide me towards some papers (perhaps in the old literature? Even
> before cryopreservation was discovered?).
> I think there might be information in papers about how to store
> osteochondral grafts, but I did not find the information I was looking for.
> Thank you in advance
> Best wishes
>
> Alida
>
> --
> Dr. Alida M. Bailleul
> Associate Professor
> Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy
> of Sciences
> www.ivpp-avianevolution.com
> & Research Associate of Paleontology, Museum of the Rockies, Montana State
> University
> Google Scholar
> <https://scholar.google.co.jp/citations?user=Yn4PuWMJ&hl=en> -
> ResearchGate
> <https://www.researchgate.net/profile/Alida_Bailleul>
>
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Re: [Histonet] Histonet Digest, Vol 226, Issue 1

[kidist Shamebo via Histonet]

On Thu, Sep 1, 2022 at 12:00 PM 
wrote:

> Send Histonet mailing list submissions to
> histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
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> or, via email, send a message with subject or body 'help' to
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>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> Today's Topics:
>
>1.  Automated ihc staining of bone (Bacon, Charles)
>
>
>
> -- Forwarded message --
> From: "Bacon, Charles" 
> To: "histonet@lists.utsouthwestern.edu"  >
> Cc:
> Bcc:
> Date: Wed, 31 Aug 2022 17:23:34 +
> Subject: [Histonet]  Automated ihc staining of bone
> When we have section lifting during IHC, we will use a concentrated Poly-L
> Lysine solution. You can dilute this solution but instead we will use a
> direct application (wet the tip of a gloved finger with the solution, and
> wipe down the slide, let dry) on one of our charged slides. This may cause
> a degree of counter stain background but always improves the IHC result for
> the pathologist. We use this for bone or heavily keratinized skin sections.
>
> Supplies:
> Poly-L: Sigma Diagnostics™ Poly-L-lysine Solution - SDP8920A
> Slides: TYPENEX Medical Microscope Slides - PS0101P
>
> Chuck Bacon, HTL(ASCP)CM
> Supervisor Histology
> Baystate Medical Center
> 361 Whitney Ave., Holyoke, MA 01040
> Telephone: 413-322-4786  Fax: 413-322-4790
> charles.ba...@baystatehealth.org
>
> -Original Message-
> From: histonet-requ...@lists.utsouthwestern.edu <
> histonet-requ...@lists.utsouthwestern.edu>
> Sent: Wednesday, August 31, 2022 1:00 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 225, Issue 14
>
> ⚠ EXTERNAL EMAIL
>
> Send Histonet mailing list submissions to
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> To subscribe or unsubscribe via the World Wide Web, visit
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> or, via email, send a message with subject or body 'help' to
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>   EXTERNAL EMAIL: This email came from outside of Baystate. Please use
> extreme caution before clicking on any links or opening any attachments.
>
> --
> Please view our annual report at http://www.bhannualreport.org
>
>
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> designated recipients named above. If you are not the intended recipient,
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Re: [Histonet] Histonet Digest, Vol 225, Issue 5--REMOVE FROM LIST

[Jill Simmonds via Histonet]

Please remove me from subscription list.

Thanks,
Jill



Jill Simmonds | Account Executive Clinical and Research Reagents
Phone: 925.586.6268 | www.biocare.net
Biocare Medical, LLC 60 Berry Drive, Pacheco CA 94553
This electronic transmission (and any attached document) contains information 
from Biocare Medical, LLC. 
They are intended only for named recipients above, and contain information that 
may be confidential or privileged. 
If you are not an intended recipient, you must not read, copy, use or disclose 
this communication. 
Please also notify the sender by replying to this message, and then delete all 
copies of it from your system.

-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 
 
Sent: Saturday, August 6, 2022 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 225, Issue 5

CAUTION: This email originated from outside of Biocare Medical. Do not click 
links or open attachments unless you recognize the sender and know the content 
is safe. Please contact the IT Help Desk if you need assistance.

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Re: [Histonet] Histonet Digest, Vol 225, Issue 5

[John O’Brien via Histonet]

To whom it concerns 
   Please contact IMEB Inc for info on refurbished Leica Slide or cassette 
printer we been selling refurbishing Leica slide and cassette printers for 15 
years,
As long as u have software and stand alone unit
I have refurbished printers with complete warranty  of 9 months
On this unit
You can use your current software to drive a refurbished slide or cassette 
Leica printer.
Regards 
IMEB Inc


> On Aug 6, 2022, at 10:13 AM, histonet-requ...@lists.utsouthwestern.edu wrote:
> 
> Send Histonet mailing list submissions to
>histonet@lists.utsouthwestern.edu
> 
> To subscribe or unsubscribe via the World Wide Web, visit
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
>histonet-requ...@lists.utsouthwestern.edu
> 
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>histonet-ow...@lists.utsouthwestern.edu
> 
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> 
> 
> Today's Topics:
> 
>   1. Re: Slide Printer (Terri  Braud)
>   2. Re: Slide Printer (Cooper, Brian)
> 
> 
> --
> 
> Message: 1
> Date: Fri, 5 Aug 2022 19:03:56 +
> From: "Terri  Braud" 
> To: "histonet@lists.utsouthwestern.edu"
>
> Subject: Re: [Histonet] Slide Printer
> Message-ID: 
> Content-Type: text/plain; charset="iso-8859-1"
> 
> We've been using our Leica IPS and IPC printers for over 15 years, first as 
> stand-alone units, and now, integrated into CoPath.  What a workhorse these 
> instruments this have been!  Their modern equivalent is almost identical, so 
> parts are readily available.  My recommendation would be to repair or replace 
> with the same. 
> 
> Terri L. Braud, HT(ASCP)
> HNL Laboratories for 
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> Ph: 215-938-3689
> Fax: 215-938-3874
> ? Honesty
> AccouNtability
> ??? AgiLity
> ??? CoLlaboration
> ? CoMpassion
> 
> -Original Message-
> From: histonet-requ...@lists.utsouthwestern.edu 
>  
> Sent: Friday, August 5, 2022 1:00 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [EXTERNAL] Histonet Digest, Vol 225, Issue 4
> 
> CAUTION: This email originated from outside Redeemer Health. Do not click 
> links or open attachments unless you recognize the sender and know the 
> content is safe. Contact our IT Support Center at 215-938-3900 with questions.
> 
> 
> Send Histonet mailing list submissions to
>histonet@lists.utsouthwestern.edu
> 
> To subscribe or unsubscribe via the World Wide Web, visit
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
>histonet-requ...@lists.utsouthwestern.edu
> 
> You can reach the person managing the list at
>histonet-ow...@lists.utsouthwestern.edu
> 
> When replying, please edit your Subject line so it is more specific than "Re: 
> Contents of Histonet digest..."
> 
> 
> Today's Topics:
> 
>   1. slide printers (Colleen Forster)
> 
> 
> --
> 
> Message: 1
> Date: Thu, 4 Aug 2022 17:15:11 -0500
> From: Colleen Forster 
> To: histonet-request 
> Subject: [Histonet] slide printers
> Message-ID:
>
> Content-Type: text/plain; charset="UTF-8"
> 
> HEllo Histonetters,
> 
> We are a research lab that has been using the Leica IPC/IPS printed for many 
> years. Today the flash motor went out in the IPS. We are now looking into 
> updating the slide printer.
> 
> We are research only and NOT hooked into any LIS system. It would be a stand 
> alone process. Can some of you share what you have and the pros/cons?
> I need to start the search for the replacement I would like.
> 
> Thank you in advance.
> 
> --
> Colleen Forster HT(ASCP)QIHC
> BLS Histology and IHC Laboratory
> Jackson Hall, Room 2-155
> 321 Church St. SE
> Minneapolis, MN 55455
> 612-626-1930
> 
> 
> --
> 
> Subject: Digest Footer
> 
> ___
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> --
> 
> End of Histonet Digest, Vol 225, Issue 4
> 
> 
> 
> 
> 
> --
> 
> Message: 2
> Date: Fri, 5 Aug 2022 20:00:01 +
> From: "Cooper, Brian" 
> To: Terri Braud ,
>"histonet@lists.utsouthwestern.edu"
>
> Subject: Re: [Histonet] Slide Printer
> Message-ID: 
> Content-Type: text/plain; charset="iso-8859-1"
> 
> Similar story here, except we went with SlideMates.  Before AB&T LIS 
> integration, we were able to have CoPath print a barcode on our cassettes 
> (Leica IPC cassette printer). The SlideMate Field Service Reps were able to 
> translate that barcode info on the slide printers (which were still 
> standalone at that poin

Re: [Histonet] Histonet Digest, Vol 225, Issue 1

[Joseph Brooks via Histonet]

Hello Anne,

Have you looked at any of the Milestone Medical processors? I have used
their benchtop processors in the past and they are great.

Regards,
Matt

On Tue, Aug 2, 2022 at 11:11 AM 
wrote:

> Send Histonet mailing list submissions to
> histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-requ...@lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-ow...@lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
>1. Small processors (Anne Murvosh)
>
>
> --
>
> Message: 1
> Date: Tue, 2 Aug 2022 15:23:37 +
> From: Anne Murvosh 
> To: "histonet (histonet@lists.utsouthwestern.edu)"
> 
> Subject: [Histonet] Small processors
> Message-ID:
> <
> mw4pr15mb4561a69b18bb95a52345398dc6...@mw4pr15mb4561.namprd15.prod.outlook.com
> >
>
> Content-Type: text/plain; charset="us-ascii"
>
> We are looking forward to ordering a new or used processor. We have the
> Sakura VIP5 however, I was wondering if there were smaller processors out
> there. We only do a max of about 60 skin specimens and the VIP is a bit
> large for that quantity. I would say a max of 100 specimens for future
> growth. Any thoughts. Thanks Anne Murvosh HT
> HIPAA Confidentiality Notice: The information and documents accompanying
> this e-mail may contain confidential information that is legally privileged
> and protected by federal and state law. The information is intended for use
> only by the entity or individual to whom it is addressed, the authorized
> recipient. The authorized recipient is obligated to maintain the
> information in a safe, secure, and confidential manner. The authorized
> recipient is prohibited from using the information for purposes other than
> intended, prohibited from disclosing the information to any other party
> unless required to do so by law or regulation, and is required to destroy
> the information after its stated need has been fulfilled. If you are in
> possession of this information, and are not the intended recipient, you are
> hereby notified that any improper disclosure, copying, or distribution of
> the information is strictly prohibited. Please notify the owner of the
> information immediately and arrange for its return or destruction.
>
>
> --
>
> Subject: Digest Footer
>
> ___
> Histonet mailing list
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> --
>
> End of Histonet Digest, Vol 225, Issue 1
> 
>
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Re: [Histonet] Histonet Digest, Vol 225, Issue 1

[Rob Rankin via Histonet]

I will request to cancel this Histonet Digest, if you are receiving them.

Respectfully,

*ROB RANKIN*   |   FOUNDER

r...@rankinbiomed.com–   DIRECT: (248) 708-0012
<+2482155390>

<https://rankinbiomed.com/>
14515 Mackey Rd, Holly, MI 48442

Equipment New <https://www.rankinwarehouse.com/collections/new-equipment>
| Equipment
Refurbished
<https://www.rankinwarehouse.com/collections/refurbished-equipment> |
Consumables <https://www.rankinwarehouse.com/pages/supplies> | Customer
Support <https://rankinbiomed.com/technical-service-can-rely-on/> | Parts
<https://www.rankinwarehouse.com/collections/parts> | Customer Reviews
<https://www.rankinwarehouse.com/pages/reviews>

<https://rankinbiomed.com/intelsint-etp-eftp/>




On Tue, Aug 2, 2022 at 1:12 PM 
wrote:

> Send Histonet mailing list submissions to
> histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-requ...@lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-ow...@lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
>1. Small processors (Anne Murvosh)
>
>
> --
>
> Message: 1
> Date: Tue, 2 Aug 2022 15:23:37 +
> From: Anne Murvosh 
> To: "histonet (histonet@lists.utsouthwestern.edu)"
> 
> Subject: [Histonet] Small processors
> Message-ID:
> <
> mw4pr15mb4561a69b18bb95a52345398dc6...@mw4pr15mb4561.namprd15.prod.outlook.com
> >
>
> Content-Type: text/plain; charset="us-ascii"
>
> We are looking forward to ordering a new or used processor. We have the
> Sakura VIP5 however, I was wondering if there were smaller processors out
> there. We only do a max of about 60 skin specimens and the VIP is a bit
> large for that quantity. I would say a max of 100 specimens for future
> growth. Any thoughts. Thanks Anne Murvosh HT
> HIPAA Confidentiality Notice: The information and documents accompanying
> this e-mail may contain confidential information that is legally privileged
> and protected by federal and state law. The information is intended for use
> only by the entity or individual to whom it is addressed, the authorized
> recipient. The authorized recipient is obligated to maintain the
> information in a safe, secure, and confidential manner. The authorized
> recipient is prohibited from using the information for purposes other than
> intended, prohibited from disclosing the information to any other party
> unless required to do so by law or regulation, and is required to destroy
> the information after its stated need has been fulfilled. If you are in
> possession of this information, and are not the intended recipient, you are
> hereby notified that any improper disclosure, copying, or distribution of
> the information is strictly prohibited. Please notify the owner of the
> information immediately and arrange for its return or destruction.
>
>
> --
>
> Subject: Digest Footer
>
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> --
>
> End of Histonet Digest, Vol 225, Issue 1
> 
>
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Re: [Histonet] Histonet Digest, Vol 224, Issue 9

[John O’Brien via Histonet]

Tom
I agree about Dr.McCormick
Regards 
John
IMEB
May he Rest In Peace

> On Jul 24, 2022, at 10:09 AM, histonet-requ...@lists.utsouthwestern.edu wrote:
> 
> Send Histonet mailing list submissions to
>histonet@lists.utsouthwestern.edu
> 
> To subscribe or unsubscribe via the World Wide Web, visit
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
>histonet-requ...@lists.utsouthwestern.edu
> 
> You can reach the person managing the list at
>histonet-ow...@lists.utsouthwestern.edu
> 
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> 
> 
> Today's Topics:
> 
>   1. The Passing Of Dr. James McCormick (Tom Pella)
> 
> 
> --
> 
> Message: 1
> Date: Sat, 23 Jul 2022 12:44:31 -0700
> From: "Tom Pella" 
> To: 
> Subject: [Histonet] The Passing Of Dr. James McCormick
> Message-ID: <01d89ecc$a128eb00$e37ac100$@tedpella.com>
> Content-Type: text/plain;charset="iso-8859-1"
> 
> I've skimmed over the posts to Histonet since late June and I haven't seen
> any post on Histonet, where the passing of Dr. James McCormick was
> mentioned. He died on June 26th, 2022. I saw this mentioned on Histology
> Professionals on FB but not here.
> 
> Here are a few links to online obituaries, the second with some rolling
> pictures:
> 
> https://www.legacy.com/us/obituaries/chicagotribune/name/james-mccormick-obi
> tuary?id=35647547
> 
> https://www.swedishhospitalfoundation.org/news/in-memoriam-dr-james-mccormic
> k
> 
> His contributions to the field of Histology can't be overstated. He invented
> the first Cryostat, the first Histology Automated Tissue Processor, the
> first Embedding Center, the first Tissue Cassette (and many other subsequent
> cassettes), the first processes to use this instrumentation. His inventions
> are in use every single day in most Histology Labs worldwide.
> 
> I only became acquainted with Dr. McCormick for a brief time later in his
> life on a product collaboration. I found him to be the consummate gentleman;
> a person in whom ideas were always bubbling to the surface; gracious and
> intelligent and witty. His passing is a great loss for this community. His
> public memorial service was held just today.
> 
> Tom Pella
> President
> Ted Pella, Inc.
> 
> 
> ??? 
> 
> 
> 
> 
> 
> 
> 
> --
> 
> Subject: Digest Footer
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> --
> 
> End of Histonet Digest, Vol 224, Issue 9
> 


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Re: [Histonet] Histonet Digest, Vol 223, Issue 14

[Patsy Ruegg via Histonet]

I used tonsil as + control.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
39037 N 11th Ave
Phoenix, AZ 85086
C 720-281-5406
prueg...@hotmail.com
Doug Ruegg
C720-281-5407
douglas...@hotmail.com


From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Friday, June 24, 2022 11:00 AM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 223, Issue 14

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
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When replying, please edit your Subject line so it is more specific
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Re: [Histonet] Histonet Digest, Vol 220, Issue 8

[Tony Henwood (SCHN) via Histonet]

Hello Jayalakshmy,

iPassport is our digital quality management system. It is a commercial product 
that allows us to collect data, record audits, document Corrective Actions, 
manage controlled documents (eg manuals), expiry data and verification of 
antibodies, staff competencies and training and equipment maintenance reports 
etc in order to aid us in meeting the ISO 15189 standard for laboratory 
accreditation.

The “History” screen is available on the Bond automated immunostainer.

This is our policy for quality assurance of out-dated antibodies, so the 
mechanics are our department’s procedure. I did not try to make it generic (too 
little time to edit ☺ ).


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) | Principal 
Scientist; Adjunct Fellow, School of Medicine, University of Western Sydney; 
Visiting Lecturer, School of Life Sciences, Faculty of Science, University of 
Technology Sydney | Histopathology
t: (02) 9845 3306 | e: 
tony.henw...@health.nsw.gov.au<mailto:tony.henw...@health.nsw.gov.au> | w: 
www.schn.health.nsw.gov.au<http://www.schn.health.nsw.gov.au>
[http://res.schn.health.nsw.gov.au/signatures/facebook.gif]<http://res.schn.health.nsw.gov.au/signatures/chw_facebook.php>
 [http://res.schn.health.nsw.gov.au/signatures/twitter.gif] 
<http://res.schn.health.nsw.gov.au/signatures/chw_twitter.php>
[http://res.schn.health.nsw.gov.au/signatures/chw.gif]
Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia
Locked Bag 4001, Westmead 2145, NSW Australia
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From: jayalakshmy p.s [mailto:psjayalaks...@gmail.com]
Sent: Friday, 18 March 2022 12:45 AM
To: Tony Henwood (SCHN) 
Subject: Re: [Histonet] Histonet Digest, Vol 220, Issue 8

Hello Henwood,
Thank you for the reply to my post with elaborate explanation and appropriate 
references. We doubted about the validity of using after expiry date. We keep 
onslide controls for every case. So with your suggestion we are confident of 
using it looking at the control. We, in developing country has not much finance 
to replenish the reagents past expiry date frequently when it is sparingly used.
Sorry to say that I didnt understand the words ipassport & history screen. Can 
you elaborate a little more about these terms?
Thanks & Regards
Jayalakshmy


On Thu, Mar 17, 2022, 6:48 AM Tony Henwood (SCHN) 
mailto:tony.henw...@health.nsw.gov.au>> wrote:
Hi Jayalakshmy,

This our policy at the Kid's hospital in Sydney:

Validation of Expired Antibodies
Usually when a new concentrated antibody is received it will have an expiry 
date of around 2 years from receipt but usually we can continue to use 
antibodies well past this expiry date.

If the antibody continues to stain control sections appropriately, with no loss 
of sensitivity and no increase in non-specific staining then its use should be 
continued. If positive control samples are deemed unsatisfactory, even if the 
antibody is within the manufacturer’s printed expiration date, evaluation of 
the clinical specimen is aborted and the test deemed invalid. The quality of 
the primary antibody is therefore not based on an expiration date, but rather 
on its performance on a case-by-case basis with appropriate positive and 
negative control samples (1).

Several authors have investigated whether the shelf-life of diagnostic 
antibodies was longer than the expiry date on the label. They found them to 
work perfectly on routine histology sections (1-4). Monoclonal antibodies 
originally supplied as culture supernatants or as ascites (neat or diluted), of 
all isotypes, as well as all of the polyclonal antibodies, produced 
satisfactory staining irrespective of their age. Notable exceptions were 
ammonium-precipitated, IgM or conjugated antibodies.

The policy at CHW is, when an antibody has reached past its expiry date, its 
control is tested to ensure that there has been no loss of sensitivity in the 
test. This is now controlled through iPassport, where a task is attached to the 
antibody requesting validation of control when the antibody is expired. This 
can be easily done by using the History Screen and looking for use of this 
antibody within the last two weeks. If results of control are acceptable, 
another task is instigated for 6 months hence.

For antibody concentrates that are received without an expiry date, a 
verification is scheduled 12 months after receipt of the antibody.

If an antibody fails to perform to expectations then a Corrective Action 
Request is instigated in iPassport and appropriate investigation is instituted.

References:
1. Savage, E. C., & DeYoung, B. R. (2010). Antibody Expiration in the Context 
of Resource Limitation What Is the Evidence Basis?. American journal of 
clinical pathology, 134(1), 60

Re: [Histonet] Histonet Digest, Vol 220, Issue 8

[Cartun, Richard via Histonet]

Well stated Tony, and thank for all this useful information.  I am hopeful that 
we can get expiration dates overturned here in the United States.  In the past, 
I have recommended a "Stability Guarantee" date to replace the "Expiration 
Date".  After that date passes it would be the responsibility of the performing 
laboratory to validate that the antibody is still working properly.  And, as 
you pointed out, this is easily accomplished with the positive control which is 
run in parallel.  Laboratories cannot no longer afford to discard expensive 
reagents, especially those that are not being produced any longer.  Also, with 
the current staffing crisis in Histology/IHC laboratories and supply-chain 
issues we need to simplify our work environment.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD
  Immunopathology/Morphologic Proteomics Laboratory
Assistant Director, Anatomic Pathology
Department of Pathology & Laboratory Medicine
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 Office
(860) 545-2204 (Fax)



-Original Message-
From: Tony Henwood (SCHN) via Histonet 
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, March 16, 2022 9:18 PM
To: jayalakshmy p.s
Cc: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Histonet Digest, Vol 220, Issue 8

EXTERNAL email from Outside HHC! Do NOT open attachments or click links from 
unknown senders.

Hi Jayalakshmy,

This our policy at the Kid's hospital in Sydney:

Validation of Expired Antibodies
Usually when a new concentrated antibody is received it will have an expiry 
date of around 2 years from receipt but usually we can continue to use 
antibodies well past this expiry date.

If the antibody continues to stain control sections appropriately, with no loss 
of sensitivity and no increase in non-specific staining then its use should be 
continued. If positive control samples are deemed unsatisfactory, even if the 
antibody is within the manufacturer’s printed expiration date, evaluation of 
the clinical specimen is aborted and the test deemed invalid. The quality of 
the primary antibody is therefore not based on an expiration date, but rather 
on its performance on a case-by-case basis with appropriate positive and 
negative control samples (1).

Several authors have investigated whether the shelf-life of diagnostic 
antibodies was longer than the expiry date on the label. They found them to 
work perfectly on routine histology sections (1-4). Monoclonal antibodies 
originally supplied as culture supernatants or as ascites (neat or diluted), of 
all isotypes, as well as all of the polyclonal antibodies, produced 
satisfactory staining irrespective of their age. Notable exceptions were 
ammonium-precipitated, IgM or conjugated antibodies.

The policy at CHW is, when an antibody has reached past its expiry date, its 
control is tested to ensure that there has been no loss of sensitivity in the 
test. This is now controlled through iPassport, where a task is attached to the 
antibody requesting validation of control when the antibody is expired. This 
can be easily done by using the History Screen and looking for use of this 
antibody within the last two weeks. If results of control are acceptable, 
another task is instigated for 6 months hence.

For antibody concentrates that are received without an expiry date, a 
verification is scheduled 12 months after receipt of the antibody.

If an antibody fails to perform to expectations than a Corrective Action 
Request is instigated in iPassport and appropriate investigation is instituted.

References:
1. Savage, E. C., & DeYoung, B. R. (2010). Antibody Expiration in the Context 
of Resource Limitation What Is the Evidence Basis?. American journal of 
clinical pathology, 134(1), 60-64.
2. Balaton, A. J., Drachenberg, C. B., Rucker, C., Vaury, P., & Papadimitriou, 
J. C. (1999). Satisfactory performance of primary antibodies beyond 
manufacturers' recommended expiration dates. Applied Immunohistochemistry & 
Molecular Morphology, 7(3), 221.
3. Argentieri, M. C., Pilla, D., Vanzati, A., Lonardi, S., Facchetti, F., 
Doglioni, C., & Cattoretti, G. (2013). Antibodies are forever: a study using 
12-26‐year‐old expired antibodies. Histopathology, 63(6), 869-876.
4. Drachenberg, C. B., Papadimitriou, J. C., Balaton, A. J., & Vaury, P. 
(2001). The total test approach to standardization of immunohistochemistry. 
Archives of pathology & laboratory medicine, 125(4), 471-471.


Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA



-

Re: [Histonet] Histonet Digest, Vol 220, Issue 8

[Tony Henwood (SCHN) via Histonet]

Hi Jayalakshmy,

This our policy at the Kid's hospital in Sydney:

Validation of Expired Antibodies
Usually when a new concentrated antibody is received it will have an expiry 
date of around 2 years from receipt but usually we can continue to use 
antibodies well past this expiry date.

If the antibody continues to stain control sections appropriately, with no loss 
of sensitivity and no increase in non-specific staining then its use should be 
continued. If positive control samples are deemed unsatisfactory, even if the 
antibody is within the manufacturer’s printed expiration date, evaluation of 
the clinical specimen is aborted and the test deemed invalid. The quality of 
the primary antibody is therefore not based on an expiration date, but rather 
on its performance on a case-by-case basis with appropriate positive and 
negative control samples (1).

Several authors have investigated whether the shelf-life of diagnostic 
antibodies was longer than the expiry date on the label. They found them to 
work perfectly on routine histology sections (1-4). Monoclonal antibodies 
originally supplied as culture supernatants or as ascites (neat or diluted), of 
all isotypes, as well as all of the polyclonal antibodies, produced 
satisfactory staining irrespective of their age. Notable exceptions were 
ammonium-precipitated, IgM or conjugated antibodies.

The policy at CHW is, when an antibody has reached past its expiry date, its 
control is tested to ensure that there has been no loss of sensitivity in the 
test. This is now controlled through iPassport, where a task is attached to the 
antibody requesting validation of control when the antibody is expired. This 
can be easily done by using the History Screen and looking for use of this 
antibody within the last two weeks. If results of control are acceptable, 
another task is instigated for 6 months hence.

For antibody concentrates that are received without an expiry date, a 
verification is scheduled 12 months after receipt of the antibody.

If an antibody fails to perform to expectations than a Corrective Action 
Request is instigated in iPassport and appropriate investigation is instituted.

References:
1. Savage, E. C., & DeYoung, B. R. (2010). Antibody Expiration in the Context 
of Resource Limitation What Is the Evidence Basis?. American journal of 
clinical pathology, 134(1), 60-64.
2. Balaton, A. J., Drachenberg, C. B., Rucker, C., Vaury, P., & Papadimitriou, 
J. C. (1999). Satisfactory performance of primary antibodies beyond 
manufacturers' recommended expiration dates. Applied Immunohistochemistry & 
Molecular Morphology, 7(3), 221.
3. Argentieri, M. C., Pilla, D., Vanzati, A., Lonardi, S., Facchetti, F., 
Doglioni, C., & Cattoretti, G. (2013). Antibodies are forever: a study using 
12-26‐year‐old expired antibodies. Histopathology, 63(6), 869-876.
4. Drachenberg, C. B., Papadimitriou, J. C., Balaton, A. J., & Vaury, P. 
(2001). The total test approach to standardization of immunohistochemistry. 
Archives of pathology & laboratory medicine, 125(4), 471-471.


Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA



-Original Message-
From: jayalakshmy p.s via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, 10 March 2022 1:27 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Histonet Digest, Vol 220, Issue 8

Hello all, I would like to know whether Immunohistochemistry markers can be 
used after its expiry date(atleast in resource poor countries). If yes, for how 
much period of time and what is the criteria to look for?
Thanks and regards
Jayalakshmy

On Wed, Mar 9, 2022, 11:30 PM 
wrote:

> Send Histonet mailing list submissions to
> histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-requ...@lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-ow...@lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> Today's Topics:
>
>1. Re: Post message to Histonet (Chenoa Hardwick)
>2. Re: formalin in OR (Greg Dobbin)
>3. posting (Chenoa Hardwick)
>4. Re: formalin in OR (John Garratt)
>
>
>
> -- Forwarded message --
> From: Chenoa Hardwick 
> To: histonet@lists.utsouthwestern.edu
> Cc:
&

Re: [Histonet] Histonet Digest, Vol 220, Issue 8

[jayalakshmy p.s via Histonet]

Hello all, I would like to know whether Immunohistochemistry markers can be
used after its expiry date(atleast in resource poor countries). If yes, for
how much period of time and what is the criteria to look for?
Thanks and regards
Jayalakshmy

On Wed, Mar 9, 2022, 11:30 PM 
wrote:

> Send Histonet mailing list submissions to
> histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-requ...@lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-ow...@lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> Today's Topics:
>
>1. Re: Post message to Histonet (Chenoa Hardwick)
>2. Re: formalin in OR (Greg Dobbin)
>3. posting (Chenoa Hardwick)
>4. Re: formalin in OR (John Garratt)
>
>
>
> -- Forwarded message --
> From: Chenoa Hardwick 
> To: histonet@lists.utsouthwestern.edu
> Cc:
> Bcc:
> Date: Tue, 8 Mar 2022 12:17:13 -0600
> Subject: Re: [Histonet] Post message to Histonet
> Please post to histonet:
>
> Job opening for histotechs (all shifts) and Pathology laboratory manager
> position (Utah)
>
> Contact che...@pathologywatch.com for inquiries.
>
> Thanks
>
> *Chenoa Hardwick*
>
> VP of Laboratory Services
>
> email: che...@pathologywatch.com 
>
> mobile: +1 972.351.0774
>
>
> <http://pathologywatch.com/>
>
>
> On Tue, Mar 8, 2022 at 12:08 PM Chenoa Hardwick  >
> wrote:
>
> > I would like to post a job opening on Histonet.
> >
> > Please see attached job description.
> >
> > Thanks
> >
> > *Chenoa Hardwick*
> >
> > VP of Laboratory Services
> >
> > email: che...@pathologywatch.com 
> >
> > mobile: +1 972.351.0774
> >
> >
> > <http://pathologywatch.com/>
> >
>
>
> 
> Secured by Paubox - HITRUST CSF certified
> https://www.paubox.com
> 
>
>
>
> -- Forwarded message --
> From: Greg Dobbin 
> To: nancy.schm...@mercyhealth.com, histonet@lists.utsouthwestern.edu
> Cc:
> Bcc:
> Date: Tue, 8 Mar 2022 16:00:14 -0400
> Subject: Re: [Histonet] formalin in OR
> Hi Nancy,
> All routine specimens in our hospital are placed in formalin in the OR.
> Breast lumps and mastectomy specimens are sent up fresh so that they arrive
> STAT (to minimize cold ischemic times) and lymph nodes for lymphoma
> protocol are also sent up fresh. [*they actually send sentinel nodes up
> fresh too- it was just less confusing*]
>
> Diagnostic Imaging will place routine needle core biopsies directly in
> formalin (e.g needle cores of breast, prostate, non-lympoma lymph nodes and
> other misc. tissue masses). Lymph node cores for lymphoma protocol and
> renal cores are sent to the lab fresh, in a container on saline soaked
> Telfa pads.
>
> Endoscopy places all of their specimens directly in formalin. The derm
> clinic will place all routine skins directly into formalin but specimens
> destined for immunofluoresence are sent up to the lab fresh in a container
> on saline soaked Telfa pads. EBUS specimens are split between Cytology and
> Histology...the histo specimens go directly into formalin.
>
> Greg
>
> --
> *Greg Dobbin*
> 1205 Pleasant Grove Rd
> RR#2 York,
> PE  C0A 1P0
>
>
> *Everything in moderation...even moderation itself**!*
>
>
>
>
> -- Forwarded message --
> From: Chenoa Hardwick 
> To: histonet@lists.utsouthwestern.edu
> Cc:
> Bcc:
> Date: Tue, 8 Mar 2022 14:19:11 -0600
> Subject: [Histonet] posting
> Please post:
>
> Job open - Salt Lake City, UT - Pathology Lab Manager
> Relocation, sign on bonus available.
>
> Please contact Chenoa Hardwick at che...@pathologywatch.com 972-351-0774
>
> *Chenoa Hardwick*
>
> VP of Laboratory Services
>
> email: che...@pathologywatch.com 
>
> mobile: +1 972.351.0774
>
>
> <http://pathologywatch.com/>
>
>
> 
> Secured by Paubox - HITRUST CSF certified
> https://www.paubox.com
> ----
>
>
>
> -- Forwarded message --
> From: John Garratt 
> To: Nancy Schmitt , "
> histonet@lists.utsouthwestern.edu" 
> Cc:
> Bcc:
> Date: Wed, 09 Mar 2022 01:28:25 +
> Subject: Re: [Histonet] formalin in OR
> I would not normally promote a product but I have seen the TissueSafe and
> SealSafe (Milestone) in use at

Re: [Histonet] Histonet Digest, Vol 216, Issue 5

[Linda Hines via Histonet]

STOP

On Sat, Nov 13, 2021, 11:00 AM 
wrote:

> Send Histonet mailing list submissions to
> histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-requ...@lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-ow...@lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> Today's Topics:
>
>1. Re: Paraffin embedding following storage in 70% alcohol
>   (John Kiernan)
>
>
>
> -- Forwarded message --
> From: John Kiernan 
> To: "histonet@lists.utsouthwestern.edu" ,
> Charles Riley 
> Cc:
> Bcc:
> Date: Fri, 12 Nov 2021 22:42:06 +
> Subject: Re: [Histonet] Paraffin embedding following storage in 70% alcohol
> Of course you are right!
> This is yet another example of an error in a procedure informally handed
> on from person to person! Always work from a book. Even a very old one will
> be OK for paraffin embedding.
> John Kiernan.
>
> https://www.schulich.uwo.ca/anatomy/people/faculty/emeriti/kiernan_john.html
> = = =
> 
> From: Charles Riley via Histonet 
> Sent: November 12, 2021 11:03 AM
> To: histonet@lists.utsouthwestern.edu 
> Subject: [Histonet] Paraffin embedding following storage in 70% alcohol
>
> I am working with a grad student on a project dealing with equine articular
> cartilage.
>
> The protocol she sent me for embedding the tissue samples goes directly
> from 70% alcohol to the embedding step in paraffin.
>
>
> Correct me if I am wrong but shouldn't the tissue be dehydrated fully and
> cleared before embedding the samples?
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ___
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Re: [Histonet] Histonet Digest, Vol 215, Issue 18

[Kathryn Perkinson via Histonet]

Open Position:
Laboratory Supervisor Histology which is part of the Division of Anatomic 
Pathology and Digital Analytics.  This position offers an opportunity to 
supervise a large laboratory with 3 shifts and weekends.  It is part of a 
larger division which includes IHC, FISH, and Digital Imaging. In this 
position, you have room to learn new technologies and become part of a dynamic 
team.  Please contact me if you have 5 years Histology experience and a BS 
degree and HT or HTL certification and supervisory experience (preferred not 
required).  Send your resume today to get started on a new role!

Kathryn R. Perkinson, BS, HTL(ASCP), CSSGB
Manager, Division of Anatomic Pathology and Digital Analytics
Box 3712, Room 4710 Duke South Clinic Building
Duke University Medical Center
Durham, NC 27710
Office: 919-684-5822
Cell: 919-603-7672

-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 
 
Sent: Sunday, October 24, 2021 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 215, Issue 18

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit

https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!OToaGQ!6PMpgI1jz4K3oUc5rBIIq3ZbAow6DL__aJ7AM4KSraakh4MbyCEZhdtqfqoCGu9PpkOcQTc$
or, via email, send a message with subject or body 'help' to
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When replying, please edit your Subject line so it is more specific than "Re: 
Contents of Histonet digest..."


Today's Topics:

   1. Histology Jobs Houston, TX (Thomas Huynh)


--

Message: 1
Date: Sun, 24 Oct 2021 14:42:16 + (UTC)
From: Thomas Huynh 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] Histology Jobs Houston, TX
Message-ID: <1278571686.377822.1635086536...@mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8

Hello Histonetter,
I am posting this for a friend:?
We are a private reference laboratory that is derm focused with 3 full time 
histotech?openings,?3 PRN histotech openings, 1 PRN transcriptionist opening, 
and 1 PRN grossing opening.? The?full time histotech?openings have shifts 
of?11pm- 7:30am, 12am- 8:30am, and?3:30am - 12pm. PRN histotech?shifts vary for 
when coverage is needed, Monday through Saturday.? Our?PRN transcriptionist 
shift varies based on need and has a start time of?5pm, Tuesday through 
Friday.? Our PRN grossing position has a start time of 10am and 2pm.??All 
shifts include a shift differential of $3/hr from 10pm - 5am.?

Thanks,Thomas

--

Subject: Digest Footer

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End of Histonet Digest, Vol 215, Issue 18
*

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Re: [Histonet] Histonet Digest, Vol 215, Issue 3

[John Frazier via Histonet]

rded message --
> From: "Hannen, Valerie" 
> To: "Histonet@lists.utsouthwestern.edu"  >
> Cc:
> Bcc:
> Date: Tue, 5 Oct 2021 16:30:46 +
> Subject: [Histonet] FW: Biopsy processing
>
>
> From: Hannen, Valerie
> Sent: Monday, October 04, 2021 2:54 PM
> To: 'histo...@list.utsouthwestern.edu'
> Subject: FW: Biopsy processing
>
>
>
> From: Hannen, Valerie
> Sent: Monday, October 4, 2021 11:03 AM
> To: 'histo...@list.utsouthwestern.edu' 
> Subject: Biopsy processing
>
>   Good Morning All...  My Pathologist is wondering if there is any
> benefit to having a separate processing run just for small biopsies?  He
> said that what is seeing now, he has no issue with, the sections are just
> as good as all the other tissue sections. He just asked for me to get the
> consensus of the group.
>
> Thank You in advance!!
>
>
>
>
> Valerie A. Hannen,MLT(ASCP),HTL,SU(FL)
> Histology Section Chief
> Parrish Medical Center
> 951 N. Washington Avenue
> Titusville, Florida 32796
> P: 321-268-6333  Ext. 7506
> F: 321-268-6149
> valerie.han...@parrishmed.com<mailto:valerie.han...@parrishmed.com>
> www.parrishmed.com<http://www.parrishmed.com>
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
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Re: [Histonet] Histonet Digest, Vol 214, Issue 14

[Kathryn Perkinson via Histonet]

that we had significant washing on  3 of 8 bone 
marrow clot sections the other day; this is not the first time so we would like 
to get to the bottom of this.   We use positively charged slides and all 8 
cases were cut and run the same morning but allowed to air dry and then bake at 
60C for 20 minutes before being run on our Bond 3 stainer.   Has anyone out 
there experienced this type of problem and if so, what were your solutions?
The repeat of the 3 cases today showed similar washing of tissue.

This hasn't just started but has occurred periodically but the pathologists 
have tried to live with it and usually we can finally get enough tissue to stay 
on after 1-2 attempts.   Suggestions include cutting and drying the slides 
overnight and/or going to a gelatinated  slide versus a sialylated slide.   We 
have been using this particular brand of positive charged slide with good 
results for several years and rarely have issues with other tissue types unless 
they are particularly bloody.

Thoughts or suggestions are greatly appreciated.


Martha Ward MT(ASCP) QIHC
Atrium Health Wake Forest Baptist





--

Message: 4
Date: Thu, 23 Sep 2021 13:16:27 -0700
From: Regan Fulton 
To: Martha Ward-Pathology ,
Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Bone marrow clot IHC tissue sections washing
Message-ID:

Content-Type: text/plain; charset="UTF-8"

Martha,

We reported our study of 15 brands of adhesive slides for "wash off" and found 
little difference among the different slides when well-fixed cell culture 
material was examined.
On the other hand, poorly-fixed breast cancer tissues did appear to adhere more 
strongly to some slides than others (TOMO being among the best).
Additional factors need to be considered, though, and I note that your baking 
procedure is different from what is recommended by many slide vendors.
In general, baking at 60-65 deg C for 1 hour is said to be optimal, although we 
did not examine that parameter specifically.

Please see our poster at 
https://urldefense.com/v3/__https://www.arrayscience.com/publications*Posters__;Iw!!OToaGQ!6kZFsSGmGAqtHSUCXGvBt6QPRHlQc7P4geZI9L6HlrVmY_FPmCDYnGHGszj4cR01HGjCd-A$
 

Best regards,



Regan
Regan Fulton, M.D., Ph.D.
CEO and Co-Founder
Array Science, LLC
475 Gate 5 Road, #100
Sausalito, CA 94965
(415) 577-7360
email: ful...@arrayscience.com


https://urldefense.com/v3/__http://www.arrayscience.com__;!!OToaGQ!6kZFsSGmGAqtHSUCXGvBt6QPRHlQc7P4geZI9L6HlrVmY_FPmCDYnGHGszj4cR01q5l4Oys$
 



On Thu, Sep 23, 2021 at 1:02 PM Martha Ward-Pathology via Histonet < 
histonet@lists.utsouthwestern.edu> wrote:

> It was brought to my attention that we had significant washing on  3 
> of 8 bone marrow clot sections the other day; this is not the first time so we
> would like to get to the bottom of this.   We use positively charged slides
> and all 8 cases were cut and run the same morning but allowed to air 
> dry and then bake at 60C for 20 minutes before being run on our Bond 3
> stainer.   Has anyone out there experienced this type of problem and if so,
> what were your solutions?The repeat of the 3 cases today showed similar
> washing of tissue.
>
> This hasn't just started but has occurred periodically but the 
> pathologists have tried to live with it and usually we can finally get
> enough tissue to stay on after 1-2 attempts.   Suggestions include cutting
> and drying the slides overnight and/or going to a gelatinated  slide versus
> a sialylated slide.   We have been using this particular brand of positive
> charged slide with good results for several years and rarely have 
> issues with other tissue types unless they are particularly bloody.
>
> Thoughts or suggestions are greatly appreciated.
>
>
> Martha Ward MT(ASCP) QIHC
> Atrium Health Wake Forest Baptist
>
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/li
> stinfo/histonet__;!!OToaGQ!6kZFsSGmGAqtHSUCXGvBt6QPRHlQc7P4geZI9L6HlrV
> mY_FPmCDYnGHGszj4cR01z2-BarQ$
>


--

Message: 5
Date: Thu, 23 Sep 2021 14:47:24 -0700
From: Bryan Llewellyn 
To: Jordan ,Histonet

Subject: Re: [Histonet] Jones' Methenamine Silver Stain for Basement
Membranes of Kidney - Issues and Questions
Message-ID: <9e1fe5b8-be5e-ae7f-ca29-e57dac959...@shaw.ca>
Content-Type: text/plain; charset=UTF-8; format=flowed

Hi,
Try the method given in StainsFile at:
https://urldefense.com/v3/__http://stainsfile.info/stain/metallic/jones.htm__;!!OToaGQ!6kZFsSGmGAqtHSUCXGvBt6QPRHlQc7P4geZI9L6HlrVmY_FPmCDYnGHGszj4cR01CJSb57Q$
 

Bryan Llewellyn


Hood, Jordan via Histonet wrote:
> Hello,
> 
> I'm new to histology (and new to histonet), and I work 

Re: [Histonet] Histonet Digest, Vol 214, Issue 1

[John O’Brien via Histonet]

Be careful 
Cost of cassette are 50 cents each when using general data systems
Pathtrade

Sent from my iPhone

> On Sep 1, 2021, at 10:18 AM, histonet-requ...@lists.utsouthwestern.edu wrote:
> 
> Send Histonet mailing list submissions to
>histonet@lists.utsouthwestern.edu
> 
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>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
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>histonet-ow...@lists.utsouthwestern.edu
> 
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> 
> 
> Today's Topics:
> 
>   1. Re: Histonet Digest, Vol 213, Issue 22 (Patsy Ruegg)
>   2. Anyone Using LaserTrack PH6 or PH8 Tissue CassettePrinter
>  General Data (Donna Emge)
> 
> 
> --
> 
> Message: 1
> Date: Tue, 31 Aug 2021 17:08:45 +
> From: Patsy Ruegg 
> To: "histonet@lists.utsouthwestern.edu"
>
> Subject: Re: [Histonet] Histonet Digest, Vol 213, Issue 22
> Message-ID:
>
> 
>
> Content-Type: text/plain; charset="us-ascii"
> 
> I would suggest using something like histogel or agar to make layers of blood 
> drops in a tube, then freeze them and make frozen sections. Sounds tricky.
> 
> Patsy Ruegg, HT(ASCP)QIHC
> Ruegg IHC Consulting
> 39037 N 11th Ave
> Phoenix, AZ 85086
> C 720-281-5406
> prueg...@hotmail.com
> Doug Ruegg
> C720-281-5407
> douglas...@hotmail.com
> 
> 
> From: histonet-requ...@lists.utsouthwestern.edu 
> 
> Sent: Tuesday, August 31, 2021 11:00 AM
> To: histonet@lists.utsouthwestern.edu 
> Subject: Histonet Digest, Vol 213, Issue 22
> 
> Send Histonet mailing list submissions to
>histonet@lists.utsouthwestern.edu
> 
> To subscribe or unsubscribe via the World Wide Web, visit
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
>histonet-requ...@lists.utsouthwestern.edu
> 
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>histonet-ow...@lists.utsouthwestern.edu
> 
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> 
> 
> --
> 
> Message: 2
> Date: Wed, 1 Sep 2021 10:49:57 -0500
> From: Donna Emge 
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Anyone Using LaserTrack PH6 or PH8 Tissue Cassette
>Printer General Data
> Message-ID:
>
> Content-Type: text/plain; charset="UTF-8"
> 
> Is anyone using to the LaserTrack PH6 or PH8 Tissue Cassette Printer from
> General Data? I am looking for information about how you like it and if
> there are any issues to consider. Pros and cons.
> 
> Donna Emge, HT(ASCP)
> Histology Laboratory Manager
> Ascension Seton Medical Center
> Austin, TX
> djemg...@gmail.com
> 
>> On Tue, Aug 31, 2021, 12:17 PM 
>> wrote:
>> 
>> Send Histonet mailing list submissions to
>>histonet@lists.utsouthwestern.edu
>> 
>> To subscribe or unsubscribe via the World Wide Web, visit
>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> or, via email, send a message with subject or body 'help' to
>>histonet-requ...@lists.utsouthwestern.edu
>> 
>> You can reach the person managing the list at
>>histonet-ow...@lists.utsouthwestern.edu
>> 
>> When replying, please edit your Subject line so it is more specific
>> than "Re: Contents of Histonet digest..."
>> 
>> 
>> Today's Topics:
>> 
>>   1. RELIA HOT Histology Job Alert!! Exciting NEW  Opportunities in
>>  Denver, CO, Chicago, IL, Gainesville, FL and San Diego, CA!
>>  These are RELIA EXCLUSIVES!! (Pam Barker)
>>   2. Cryostat vacuums (Wooten, Jennifer)
>>   3. need some research help (Horner, Roberta J)
>> 
>> 
>> --
>> 
>> Message: 1
>> Date: Mon, 30 Aug 2021 14:04:15 -0400
>> From: "Pam Barker" 
>> To: "Histopeeps Histonet" 
>> Subject: [Histonet] RELIA HOT Histology Job Alert!! Exciting NEW
>>Opportunities in Denver, CO, Chicago, IL, Gainesville,  FL and San
>>Diego, CA!  These are RELIA EXCLUSIVES!!
>> Message-ID: 

Re: [Histonet] Histonet Digest, Vol 213, Issue 22

[Patsy Ruegg via Histonet]

I would suggest using something like histogel or agar to make layers of blood 
drops in a tube, then freeze them and make frozen sections. Sounds tricky.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
39037 N 11th Ave
Phoenix, AZ 85086
C 720-281-5406
prueg...@hotmail.com
Doug Ruegg
C720-281-5407
douglas...@hotmail.com


From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Tuesday, August 31, 2021 11:00 AM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 213, Issue 22

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Re: [Histonet] Histonet Digest, Vol 213, Issue 6

[Wooten, Jennifer via Histonet]

We just recently purchased the Epredia NX50, which we use with our older 
HM525s. We love it, I just wish they would make height adjustability a standard 
option on them!

Jennifer Wooten, BA, BS, HTL (ASCP)CM
Pronouns: she/her/hers
Technical Supervisor | Anatomic Pathology | University Hospital
jennifer.woo...@tricore.org
Desk: 505.272.5486 | Fax: 505.272.0240
TriCore Reference Laboratories
2211 Lomas Blvd NE
Albuquerque, NM 87106
www.tricore.org

-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Monday, August 9, 2021 11:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 213, Issue 6

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Re: [Histonet] Histonet Digest, Vol 212, Issue 14

[Izak Dimenstein via Histonet]

In contrast to gynecological cytology, nongynecological cytology (NGYN) does 
not have a separate CPT code for screening.
Details in my book's Procedural Coding in Anatomic Pathology Cytopathology 
section, pp. 162-176.
Izak Dimenstein

From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Tuesday, July 27, 2021 1:00 PM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 212, Issue 14

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Re: [Histonet] Histonet Digest, Vol 212, Issue 7

[richard.wild--- via Histonet]

Message: 1
Date: Wed, 14 Jul 2021 22:03:24 + (UTC)
From: Cheryl 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] PathCentre (ThermoShandon) protocols - help?
Message-ID: <7393119.276682.1626300204...@mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8


Help?? Starting a refurbished PathCentre and was hoping someone could share a 
biopsy protocol for xylene processing?? Routine human biopsies with specials 
and some IHC and molecular down the road.
Open to all -?
Much appreciated!!
Cheryl Kerry, HT(ASCP)


Hi Cherryl

it's a good machine (we have 3 of them)

Here under some "official" protocole

If unreadable, i'll send you pdf - just ask for it

Richard Wild

-

SHANDON PATHCENTRE PROCESSING SCHEDULE
RAPID BIOPSY PROGRAMME TOTAL DURATION 1hr 14mins
STEP REAGENT CONC
%
TEMP
°C
VAC/PRES TIME
Hrs:Mins
DRAIN AGIT
1 Formalin 10 45 V 00:01 15 S
2 Formalin 70 45 V 00:01 15 S
3 Alcohol 95 45 V 00:01 15 S
4 Alcohol 100 45 V 00:01 15 S
5 Alcohol 100 45 V 00:01 15 S
6 Alcohol 100 45 V 00:01 15 S
7 Alcohol 100 45 V 00:01 15 S
8 Xylene 45 V 00:01 15 S
9 Xylene 45 V 00:01 15 S
10 Xylene 45 V 00:01 15 S
11 Wax 60 V 00:01 60 S
12 Wax 60 V 00:01 60 S
13 Wax 60 V 00:01 60 S
14 Wax 60 V 00:01 60 S

SHANDON PATHCENTRE PROCESSING SCHEDULE
RAPID PROCESSING TOTAL DURATION 0hr 56mins
STEP REAGENT CONC
%
TEMP
°C
VAC/PRES TIME
Hrs:Mins
DRAIN AGIT
1 Formalin 10 45 V 00:02 15 S
2 Formalin 70 45 V 00:02 15 S
3 Alcohol 95 45 V 00:02 15 S
4 Alcohol 00:00 S
5 Alcohol 00:00 S
6 Alcohol 100 45 V 00:02 15 S
7 Alcohol 100 45 V 00:02 15 S
8 Xylene 00:00 S
9 Xylene 45 V 00:02 15 S
10 Xylene 45 V 00:02 15 S
11 Wax 60 00:00 S
12 Wax 60 00:00 S
13 Wax 60 V 00:02 60 S
14 Wax 60 V 00:02 60 S

SHANDON PATHCENTRE PROCESSING SCHEDULE
LARGE SURGICALS TOTAL DURATION 22hrs 34mins
STEP REAGENT CONC
%
TEMP
°C
VAC/PRES TIME
Hrs:Mins
DRAIN AGIT
1 Buffered Formalin 10 A N 02:00 15 S
2 Alcohol Formalin 10 A N 02:00 15 S
3 90% IMS * 60 A V 01:30 15 S
4 IMS 80 A V 01:30 15 S
5 IMS A V 01:30 15 S
6 IMS A V 01:30 15 S
7 Xylene/IMS A V 01:30 15 S
8 Xylene A V 01:30 30 S
9 Xylene A V 01:30 60 S
10 Xylene A V 01:30 60 S
11 Wax 60 V 01:00 120 S
12 Wax 60 V 01:00 120 S
13 Wax 60 V 01:00 120 S
14 Wax 60 V 01:30 120 S





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Re: [Histonet] Histonet Digest, Vol 212, Issue 1

[Wooten, Jennifer via Histonet]

We surface decal with decal I for about 20-30 minutes (as long as you don't 
need to do any molecular studies). You can usually get 3-4 good sections before 
its crunchy again.

Jennifer Wooten, BA, BS, HTL (ASCP)CM
Pronouns: she/her/hers
Technical Supervisor | Anatomic Pathology | University Hospital
jennifer.woo...@tricore.org
Desk: 505.272.5486 | Fax: 505.272.0240
TriCore Reference Laboratories
2211 Lomas Blvd NE
Albuquerque, NM 87106
www.tricore.org

-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Thursday, July 1, 2021 11:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 212, Issue 1

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Re: [Histonet] Histonet Digest, Vol 211, Issue 7

[Patsy Ruegg via Histonet]

The blue stain from prussian blue is a chemical reaction and indicates that 
there is a lot of iron in the section, you would not want it not to stain some 
of the iron there would you? I agree with John, take an adjacent section for a 
general stain.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
39037 N 11th Ave
Phoenix, AZ 85086
C 720-281-5406
prueg...@hotmail.com
Doug Ruegg
C720-281-5407
douglas...@hotmail.com


From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Monday, June 7, 2021 11:00 AM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 211, Issue 7

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Re: [Histonet] Histonet Digest, Vol 208, Issue 14 Best Slides for Leica IP S Slide Printers

[Steve McClain via Histonet]

Be careful.
 Look at the slides before you commit.
The least expensive may also be the dirtiest. So stack up 12 slides and look 
through them. If they look cloudy or dirty.  Nix

Do the drop test. A slide should be able to survive being dropped to the floor 
from waist high.

Choose on price after those two tests.
Just saying,

Steve
Steve A. McClain, MD
631-361-4000  Cell 631-926-3655

On Mar 27, 2021, at 13:11, histonet-requ...@lists.utsouthwestern.edu wrote:

Best Slides for Leica IP S Slide Printers
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Re: [Histonet] Histonet Digest, Vol 208, Issue 4

[Ashwin Ashwin via Histonet]

Hello Everyone,
PM me for Histo positions in Florida

Thanks
Ashwin


-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 
 
Sent: Saturday, March 6, 2021 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: {EXTERNAL} Histonet Digest, Vol 208, Issue 4

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Re: [Histonet] Histonet Digest, Vol 208, Issue 1

[John Frazier via Histonet]

Have you considered any Digital Imaging platforms such as Roche's DP200 or
Lieca's Aperio? Both of these systems provide superior imaging and you may
even be able to build your own algorithms for  immunofluourescent
multiplexing. I am not a vendor but have seen both of these systems and
evaluated them. The Roche DP 200 can scan the and give you X,Y,and Z
imaging. You can literally focus up and down on the image to see layers.
Just a thought.

*John Frazier*

*MT(ASCP), MBA*
*Lean 6 Six Sigma Black Belt*
*Healthcare Consultant*
*C - 302-310-7567*
*H - 704-847-0566*
*wilfong1...@gmail.com *




On Mon, Mar 1, 2021 at 1:00 PM 
wrote:

> Send Histonet mailing list submissions to
> histonet@lists.utsouthwestern.edu
>
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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> histonet-ow...@lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> Today's Topics:
>
>1. Any comments on your usage of the following slide scanners?
>   (Katherine Bummer)
>
>
>
> -- Forwarded message --
> From: Katherine Bummer 
> To: "histonet@lists.utsouthwestern.edu"  >
> Cc:
> Bcc:
> Date: Mon, 1 Mar 2021 17:23:26 +
> Subject: [Histonet] Any comments on your usage of the following slide
> scanners?
> Hello Histonetters,
>
> I am looking for some straight forward comments on a few slide scanners:
>
> * Zeiss Z1,
>
> * AkoyaBio Vectra Polaris and the
>
> * 3DHisTech P250FL.
>
> *
>
> I do almost exclusively immunofluourescent multiplexing, generally with
> 4-5 targets and am looking at these scanners.  I have had virtual demo's
> but no live demos and am seeking EXCELLENT image quality as I will need to
> be quantifying several targets within a sample.
>
> Just hoping if you are using one of these or have in the past that you can
> give any good feedback on your experience with image quality.  You can also
> email me directly if you prefer either to my personal email below or to my
> work email that is on this post.
>
> Thank you!
>
>
>
> Best regards,
>
> Katherine Bummer
> bumkat0...@gmail.com
>
>
> This message is intended solely for the designated recipient(s). It may
> contain confidential or proprietary information and may be subject to
> attorney-client privilege or other confidentiality protections. If you are
> not a designated recipient you may not review, copy or distribute this
> message. If you receive this in error, please notify the sender by reply
> e-mail and delete this message. Thank you.
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Re: [Histonet] Histonet Digest, Vol 207, Issue 7

[John Frazier via Histonet]

 [Histonet] Motic EasyScan Pro and Pro 6 slide scanners
> Anyone out there using either the Motic EasyScan Pro and Pro 6 slide
> scanners, maybe even the FS LIVE scanner version?
> I would love to have some feedback on any of these instruments as well as
> experience with the company service.
> thanks,
> Denise
>
> Denise M. Long, MS, HTL (ASCP) QIHC
> University of Connecticut
> Department of Pathobiology
> Connecticut Veterinary Medical Diagnostic Laboratory
> Histology Laboratory
> Storrs, Connecticut 06269-3089
> (860) 486-0851
>
>
>
>
> -- Forwarded message --
> From: John Frazier 
> To: raestask 
> Cc: Histonet@lists.utsouthwestern.edu
> Bcc:
> Date: Tue, 9 Feb 2021 15:21:26 -0500
> Subject: Re: [Histonet] H&E Stainers
> Dako has one
>
> Sent from my iPhone
>
> > On Feb 9, 2021, at 12:25 PM, raestask  wrote:
> >
> > Is there a stainer other than Ventana's that has all the reagents in
> prefilled bottles?Rae Ann Staskiewicz HT(ASCP)Sent from my Verizon, Samsung
> Galaxy smartphone
>
>
>
>
>
> -- Forwarded message --
> From: s h 
> To: John Frazier 
> Cc: raestask , histonet@lists.utsouthwestern.edu
> Bcc:
> Date: Tue, 9 Feb 2021 13:49:55 -0700
> Subject: Re: [Histonet] H&E Stainers
> The Dako Artisan
>
> > On Feb 9, 2021, at 1:39 PM, John Frazier via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
> >
> > Dako has one
> >
> > Sent from my iPhone
> >
> >> On Feb 9, 2021, at 12:25 PM, raestask  wrote:
> >>
> >> Is there a stainer other than Ventana's that has all the reagents in
> prefilled bottles?Rae Ann Staskiewicz HT(ASCP)Sent from my Verizon, Samsung
> Galaxy smartphone
> >
> > ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> -- Forwarded message --
> From: Amanda Coscetti 
> To: histonet@lists.utsouthwestern.edu
> Cc:
> Bcc:
> Date: Tue, 9 Feb 2021 16:48:30 -0500
> Subject: [Histonet] Dragon
> Does anyone use Dragon while grossing? We are looking at switching to
> Dragon and wanted some feedback for use in pathology.
> Currently, we use M*Modal for dictations and then have transcriptionists
> type all of the information in Cerner reports.
>
> Thanks!!
> Amanda
>
>
>
>
>
>
> -- Forwarded message --
> From: Cristi Rigazio 
> To: Amanda Coscetti 
> Cc: histonet@lists.utsouthwestern.edu
> Bcc:
> Date: Tue, 9 Feb 2021 17:01:54 -0500
> Subject: Re: [Histonet] Dragon
> I used it exclusively in GI lab for years and loved it but we only did
> small biopsies, so it was super simple.  We use it now throughout the
> system I work in and there are a variety of opinions.  Some of the PAs love
> it and some really struggle.  Same with the pathologists.  So we live in
> dual worlds with both Dragon and MModal.  If you would like to contact me
> separately I am happy to discuss!
>
> Thanks
> Cristi
>
> > On Feb 9, 2021, at 4:56 PM, Amanda Coscetti via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
> >
> > Does anyone use Dragon while grossing? We are looking at switching to
> Dragon and wanted some feedback for use in pathology.
> > Currently, we use M*Modal for dictations and then have transcriptionists
> type all of the information in Cerner reports.
> >
> > Thanks!!
> > Amanda
> >
> >
> > ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> -- Forwarded message --
> From: Cheryl 
> To: "histonet@lists.utsouthwestern.edu"  >
> Cc:
> Bcc:
> Date: Tue, 9 Feb 2021 17:07:26 -0600
> Subject: [Histonet] Cost per slide accounting (Rene Buss?)
> Hi guys - have a lecture to prep for PA students and hope to impress on
> them their part in quality and cost control.
>
> Does anyone have a cool visual chart or metric that drills down including
> CPT reimbisrsement through cost per block/slide with tech and PA time in
> the production accounting?
>
> If my memory serves, Rene Busa had a plethora of great info in this realm
> - are you still hanging out, sir??  Would love to hear from you
>
> And anyone else who might help..?
>
> Thanks in advance.
>
> Cheryl Kerry
>
> Please excuse typos-sent from a phone.
>
>
>
>
>
> -- Forwarded message --
>

Re: [Histonet] Histonet Digest, Vol 207, Issue 3 Bouin's vs Vinegar

[Steve McClain via Histonet]

If the desire is to fix tissue marking dyes on tissue, 5 % acetic acid 
(vinegar) works well and does not stain or alter/fix the tissue like Bouin's 
fixative.

Thanks,
Steve
Steve A. McClain, MD
631 361-4000 cell 631 926-3655


-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 
[mailto:histonet-requ...@lists.utsouthwestern.edu] 
Sent: Friday, February 5, 2021 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 207, Issue 3

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Today's Topics:

   1. Re: Histonet Digest, Vol 207, Issue 2 (Luigi Mascio)


--

Message: 1
Date: Thu, 4 Feb 2021 22:36:26 +
From: Luigi Mascio 
To: "histonet@lists.utsouthwestern.edu"
    
Subject: Re: [Histonet] Histonet Digest, Vol 207, Issue 2
Message-ID:



Content-Type: text/plain; charset="iso-8859-1"

Buy Bouin's from a consumable company and pour it in your own spray bottle!

Luigi M. Mascio
BS HT (ASCP)
Technical Director
lu...@medequipsource.com
724-687-7342 direct number
724-369-1604 ext. 102
412-216-0496  cell


For a great resource visit medequipsource.com, especially our blog with 
histology tips of the week.

MES?Premium Services:
. New Histology and Pathology Products
. High Quality Remanufactured Equipment
. Signature Preventative Maintenance Programs
. Lab Design & Workflow Layout
. Remanufacture Existing Equipment
. Innovative Financing Programs
. Service Contracts (*Limited Area)
. We carry all major OEM brands
. 
-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 
 
Sent: Thursday, February 4, 2021 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 207, Issue 2

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Re: [Histonet] Histonet Digest, Vol 207, Issue 2

[Luigi Mascio via Histonet]

Buy Bouin's from a consumable company and pour it in your own spray bottle!

Luigi M. Mascio
BS HT (ASCP)
Technical Director
lu...@medequipsource.com
724-687-7342 direct number
724-369-1604 ext. 102
412-216-0496  cell


For a great resource visit medequipsource.com, especially our blog with 
histology tips of the week.

MES Premium Services:
. New Histology and Pathology Products
. High Quality Remanufactured Equipment
. Signature Preventative Maintenance Programs
. Lab Design & Workflow Layout
. Remanufacture Existing Equipment
. Innovative Financing Programs
. Service Contracts (*Limited Area)
. We carry all major OEM brands
. 
-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 
 
Sent: Thursday, February 4, 2021 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 207, Issue 2

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Re: [Histonet] Histonet Digest, Vol 206, Issue 14

[Olszewski, Dawn via Histonet]

We use the red bag method after we pull out any we want to use as controls and 
after Oncology pulls out any they want for Tumor bank.


Dawn Olszewski
Pathology Manager - Histotechnologist
Laboratory

[cid:MasterLogo_190x61_362365a5-c941-4f16-9f99-9484fd6a6078.png]

South Georgia Medical Center
2501 N. Patterson St.
Valdosta, GA 31602
229-259-4830 (O) | 229-560-6191 (M)
dawn.olszew...@sgmc.org | sgmc.org

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  [cid:twitter_32x32_5a3a5eeb-77c7-4a3c-aa09-b69b41f32bd2.png] 
   
[cid:linkedin_ln_32x32_bf1e5dc9-b886-480a-8c8b-ef8a1ae87168.png] 
   
[cid:youtube_play_32x32_572cd3ed-390b-48e0-8845-ba1f81953de3.png] 









From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Wednesday, January 27, 2021 1:00 PM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 206, Issue 14

WARNING: This email originated outside the SGMC’s email system.
DO NOT CLICK: Links or attachments unless you recognize the sender and know the 
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Today's Topics:

   1. paraffin block disposal (Kuhnla, Melissa)


--

Message: 1
Date: Tue, 26 Jan 2021 18:08:18 +
From: "Kuhnla, Melissa" 
To: "'histonet@lists.utsouthwestern.edu'"

Subject: [Histonet] paraffin block disposal
Message-ID: <6ec7f8d26bb849f08feccdd00dc65...@chsli.org>
Content-Type: text/plain; charset="us-ascii"

Good Afternoon All,
Asking for a colleague,. How are sites disposing of old formalin fixed paraffin 
embedded surgical blocks?  Garbage?  Red bag?

Thank you :)

Melissa Kuhnla
Lead Medical Technologist
for IHC and FISH testing
Regional Laboratory Services
Good Samaritan Hospital
631-609-2551



--

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Re: [Histonet] Histonet Digest, Vol 206, Issue 14

[John Frazier via Histonet]

Remember to consider HIPPA if there are any potential identifiers on the
blocks.

*John Frazier*

*MT(ASCP), MBA*
*Lean 6 Six Sigma Black Belt*
*Healthcare Consultant*
*C - 302-310-7567*
*H - 704-847-0566*
*wilfong1...@gmail.com *




On Wed, Jan 27, 2021 at 1:00 PM 
wrote:

> Send Histonet mailing list submissions to
> histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-requ...@lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-ow...@lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> Today's Topics:
>
>1. paraffin block disposal (Kuhnla, Melissa)
>
>
>
> -- Forwarded message --
> From: "Kuhnla, Melissa" 
> To: "'histonet@lists.utsouthwestern.edu'" <
> histonet@lists.utsouthwestern.edu>
> Cc:
> Bcc:
> Date: Tue, 26 Jan 2021 18:08:18 +
> Subject: [Histonet] paraffin block disposal
> Good Afternoon All,
> Asking for a colleague,. How are sites disposing of old formalin fixed
> paraffin embedded surgical blocks?  Garbage?  Red bag?
>
> Thank you :)
>
> Melissa Kuhnla
> Lead Medical Technologist
> for IHC and FISH testing
> Regional Laboratory Services
> Good Samaritan Hospital
> 631-609-2551
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
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Re: [Histonet] Histonet Digest, Vol 206, Issue 1 New Tissue Processor? (Sandra Cheasty)

[Steve McClain via Histonet]

While (conceptually) looking forward to the virtues of new and improved modern 
tissue processors w WiFi and SMS messaging capability, I fully expect to be 
operating the 1979 K-series for years to come.

I reckon we may be behind the times, but with regular annual maintenance, our  
VIP5 and even more reliable K-series VIP processors refuse to die. These 
run/process 6 days a week and twice a day, if/when rapid processing is needed.

PS we also have two spare operational (but slightly hacked into) K-series 
processors from when we were contemplating an experiment in cold processing 
using refrigerated alcohols and xylenes to preserve some obscure research 
antigens. In hindsight, it was a goofy academic exercise and soon was abandoned 
and rarely mentioned, after we (meaning Joel) adjusted the antigen retrieval.

Now seriously, has anyone else ever modified their  tissue processor with a 
Saws-all? Please reply confidentially.

Steve
Steve A. McClain, MD
631-361-4000  Cell 631-926-3655

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Re: [Histonet] Histonet Digest, Vol 205, Issue 2

[Izak Dimenstein via Histonet]

In my experience and opinion, grossing and embedding are the main sources of 
extraneous tissues ("floaters"). This issue is discussed in detail in the 
section "Root Cause Analysis for floaters prevention during grossing and 
embedding in surgical pathology histology laboratory" in my book Grossing 
Technology pp. 254-265.

Izak Dimenstein

From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Friday, December 4, 2020 1:00 PM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 205, Issue 2

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Re: [Histonet] Histonet Digest, Vol 204, Issue 6

[Krista Ranalli via Histonet]

Good morning All,
I have had my fair share of nails falling off. I have found sometimes
letting the slides air dry over night and staining then the next day helps.
Have you tried thus yet?
Respectfully
Krista

On Sat, Nov 14, 2020, 3:00 AM 
wrote:

> Send Histonet mailing list submissions to
> histonet@lists.utsouthwestern.edu
>
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>
> You can reach the person managing the list at
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>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> Today's Topics:
>
>1. Toenail staining issues (Charles Riley)
>
>
>
> -- Forwarded message --
> From: Charles Riley 
> To: "histonet@lists.utsouthwestern.edu"  >
> Cc:
> Bcc:
> Date: Fri, 13 Nov 2020 14:51:29 +
> Subject: [Histonet] Toenail staining issues
> >From time to time, we have issues with our sections of toenail falling
> off the slide during staining.
>
> Our H&E sections usually stain without issue but when we do our special
> stains (PASF and GMS) the tissue falls off routinely.
>
> We use charged slides and if a section falls off, we coat the recut slide
> with albumin to help get extra adherence. However sometimes even this does
> not help the tissue stick through the whole process (especially on GMS).
>
>
> Does anyone have any additional suggestion we could try to get the
> sections to adhere better throughout the process?
>
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Re: [Histonet] Histonet Digest, Vol 203, Issue 21

[Eddie Martin via Histonet]

Hi Histonet and W.A. Hassan,

Supposing you have a slide volume of 15-20k slides per year & currently use
a Dako instrument, but would like feedback on whether to get a:
  * Bond Max, or
  * Roche Ultra

My response would depend more than what your yearly slide volume is.
  * If...continually loading of slides while having access
to the reagent carousel is important to you, then I'd say Roche Ultra has
the advantage.
  *If...having more control of your IHC protocols to change
your retrieval conditions, or even loading one time retrieval conditions
without saving it as a new protocol, then I'd say Leica Bond has the
advantage.

It really depends on what your lab will be staining is what I'm getting
to.
Another factor to include is ease of use.  Roche Ultra is literally a lot
easier to use than Leica Bond, and Leica Bond is easier to use than the old
Dako's or Dako Flex or similarly Thermo or Biocare IHC instruments.
   * If you're staining odd protocols and want an
instrument staining biomarker assays at variant conditions outside the
norm:
 (ie: staining 50-100 micron frozen sections looking
for nerve bundles, or trying to perform FISH protocols on the instrument
without an installed FISH protocol on the instrument), then Leica is the
choice.

quasi molecular staining:
1.   if your lab is interested in using m-RNA
oligo probes, choose Leica, as they have an easier setup to using different
vendors other than their own mRNA detection.
2.   if your physician staff prefers to see
probes with a matte background, only showing positive probe staining
(though using an older, yet very robust detection method), then Roche Ultra
has the advantage.

If you're at a reference Lab and molecular performing testing that used to
be molecular based, but is now available via IHC, then Roche Ultra has a
huge advantage, particularly for the therapeutic and theranostic biomarkers
commercially available.

Other factors to consider in your choice:
*Cost
* footprint of the instrument in your lab
   * disposing of waste
   * daily, weekly & monthly maintenance of the instrument
   * cost of service.  Using a third party biomedical group
to repair or PM the instrument removes the IVD status of the instrument,
especially if you're staining any Class II's or higher, like CD117, or ER,
PR, Her2Neu...etc.
***Its ok if you're just
using it for research if this doesn't apply.

I hope this list helps you think what your team would like to use.  if your
pathologist and additional primary stakeholders would like to contact me...
you can.
I previously was a application specialist for IHC/probes with Leica
Microsystems up to 8 years ago.  However, I use a lot of Roche family of
instruments in my lab, as I'm a contracted lead for a well known
anatomic/clinical pathology laboratory.

Best regards,
Eddie Martin, HT, HTL, QIHC

On Thu, Oct 29, 2020 at 1:00 PM 
wrote:

> Send Histonet mailing list submissions to
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>
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>
> You can reach the person managing the list at
> histonet-ow...@lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> Today's Topics:
>
>1. Selection of IHC system (warda hassan)
>2. Re: Selection of IHC system (Tina Van Meter)
>
>
>
> -- Forwarded message --
> From: warda hassan 
> To: "histonet@lists.utsouthwestern.edu"  >
> Cc:
> Bcc:
> Date: Wed, 28 Oct 2020 22:48:14 +0400
> Subject: [Histonet] Selection of IHC system
> Dear Histonet
>
> Our lab is looking to purchase a new IHC system.
> Currently we are working with Dako.
> I would request your valuable feedback on :-
>
> 1- Bond max ( Leica)
> 2- Bench Mark Ultra (Roche Diagnostic)
>
> Workload is 15,000-20,000 slides/year.
>
> Many thanks in advance for your help.
>
> Kind regards
> W.A Hassan
>
>
>
>
> -- Forwarded message --
> From: Tina Van Meter 
> To: warda hassan 
> Cc: Histonet@lists.utsouthwestern.edu
> Bcc:
> Date: Wed, 28 Oct 2020 23:08:07 -0400
> Subject: Re: [Histonet] Selection of IHC system
> Bond Max
>
> On Wed, Oct 28, 2020, 2:53 PM warda hassan via Histonet <
> histonet@lists.utsouthwe

Re: [Histonet] Histonet Digest - Nikon NIS Elements

[Jennifer White via Histonet]

Amy,
I have been using Nikon's NIS Elements D imaging software for several years 
along with a Nikon DS-Fi1 digital camera to capture images of various 
histological slides, as well as small whole-mount specimens on a dissecting 
scope. I have been happy with its ease of use and the images that it captures. 
I have found it to be fairly user-friendly and easy to manipulate, at least for 
my purposes. You do have to calibrate it with your particular scope to take 
measurements, and I required some tech support to do so. There is a nice set of 
annotation and measurement tools including length, area, radius, counts, and 
angles. I have experienced minor trouble exporting data into Excel, but with 
some fussing I have been able to successfully create data files from 
measurements. I have used images and measurements in both teaching and 
research, and would recommend the software for basic image capturing and 
analysis.

Jennifer White
East Stroudsburg University

On 8/28/20, 1:00 PM, "histonet-requ...@lists.utsouthwestern.edu" 
 wrote:

Today's Topics:

   1. NIS Elements (Amy Lee)
--

Message: 1
Date: Thu, 27 Aug 2020 10:21:59 -0700
From: Amy Lee 
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] NIS Elements
Message-ID:

Content-Type: text/plain; charset="UTF-8"

Hello,
We used to use Image-Pro Premier to capture and analyze images. Does anyone
use NIS Elements with Nikon to do image capture and analyze? How do you
feel about it? If you used both of them, could you let me know what you
think? We need to make a decision for a new purchase.

Thank you,

Amy


--

Subject: Digest Footer

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End of Histonet Digest, Vol 201, Issue 20
*

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Re: [Histonet] Histonet Digest, Vol 201, Issue 15

[Izak Dimenstein via Histonet]

The rotator cuff/joint study is an interesting project. I wish I had an 
encounter with such a specimen before I’d written Grossing Bones: Principles, 
Techniques and Instruments book (Amazon.com). The problem is not in fixation, 
or even in a saw, but rather in getting the initial slab/s which preserve the 
intimate bone/soft tissue relationships. The key is in reliable immobilization 
before the cut. The technique is similar to a bone tumor with adjacent soft 
tissue.  EXAKT is excellent, but I would start with a hand saw as more 
manageable. Then you would see how it goes (cost of the saw, space for it, 
etc.). For details idimenst...@hotmail.com.


From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Saturday, August 22, 2020 1:00 PM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 201, Issue 15

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Re: [Histonet] Histonet Digest, Vol 201, Issue 3 Update info. on old topic?

[Steve McClain via Histonet]

Tim, Paula and Jeanine
I have little experience w slide scanning, yet I have captured nearly 1.8M 
slide images, mostly on film coverslipped slides. One year ago we switched back 
to glass.
1) I suspect what Tim describes may be due to the fact that the refractive 
index of film differs from glass.
We used film for nearly 20 years and for 4  years I could never get a sharp 
high power image. This was most noticeable when using 40x lens designed for 
glass cover slips.
About 16 years ago, we found an Olympus 40x lens w a correction collar allowing 
for adjustment/ focusing suitable for coverslips of different thickness but 
useful also with either film or glass. The lens was expensive ($3600 if memory 
serves me).

See 
https://www.edmundoptics.com/p/olympus-uplxapo-40x-objective-with-correction-collar/43026/

Perhaps the scanner manufacturer has a similar solution?

2) we were especially diligent (mono-maniacal) about using fresh reagents to 
keep water out of the last clearing reagent step-film has a nasty reputation 
for delaminating over time and pulling the sections off the glass. Several 
major academic centers have years and hundreds of thousands of slides now 
completely useless due to delamination.

3) film has problems with certain specimens routinely (bone and toenails and 
the thick cornified layer on volar skin) not cover slipping well leaving 
bubbles and ‘cornflakes’ or brown spots over the tissue. Cornflake artifact 
also occurs w water in the last reagent and may be seen on obscenely humid days.

4) film scratches readily when you stack slides or file them too tightly. Film 
Slides can be filed immediately after exam.  Glass generally needs to wait 2 
days before filing or one can glue up a brick of slides by filing too soon.

5) Film is faster and dries faster but glass is sharper and produces better 
images for my purposes (we switched back to glass 1 year ago- and the Leica 
model works perfectly well)

6) our old SCA film coverlipper generated more fumes than the new Leica. 
However all new instruments produce less fumes than older models.

7) glass coverslips are better stored in a desiccator/dry environment, before 
use.

8) This will sound stupid, yet not all coverslips of clean.- most (3/4) cheap 
coverslip glass is dirty and cannot be used in our lab where most/every slide 
is imaged. To determine this pick up an entire stack of coverslips and look 
through them. If they look clear-good to go; if they appear cloudy, find a new 
supplier.

9) glass coverslippers are finickyabout viscosity and volume of the mounting 
media. Once dialed in, we didn’t change media and use one brand and only one.

10) Not all film coverslip rolls are satisfactory either. We had the best luck 
w Brand S film and did not switch.

Hope these observations help.
Steve
Steve A. McClain, MD
631-361-4000  Cell 631-926-3655

Good morning!

I need to provide some information to my team about film vs. glass 
coverslipping. I have a lot of positive info. regarding film but are any cons? 
And I need to come up wit the pros of glass over film. Anyone with extended 
experience have some current information for me?


Message: 3
Date: Wed, 5 Aug 2020 13:00:37
I am interested in this too, with my main question being the quality of digital 
images from film coverslipped slides.
Paula Keene Pierce, BS, HTL
--

Message: 6
Date: Wed, 5 Aug 2020 15:38:14 +
From: "Morken, Timothy" 
To: "Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP)" 
Cc: Histonet 
Subject: Re: [Histonet] Update info. on old topic?
Message-ID:
   


Content-Type: text/plain; charset="us-ascii"

Hi Jeanine!

We are using film coverslipping exclusively now and scanning all slides. We 
moved to film because it dries almost instantly and can be scanned right away. 
It has worked very well. The only issues, and not specific to film 
coverslipping,  are that some slides with low contrast sometimes are out of 
focus. That is primarily IHC slides with low contrast counter stain and low 
contrast specific staining.

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

-Original Message-
From: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) via Histonet 

Sent: Wednesday, August 05, 2020 5:40 AM
To: 'histonet@lists.utsouthwestern.edu' 
Subject: [Histonet] Update info. on old topic?

Good morning!

I need to provide some information to my team about film vs. glass 
coverslipping. I have a lot of positive info. regarding film but are any cons? 
And I need to come up wit the pros of glass over film. Anyone with extended 
experience have some current information for me?

Thanks very much,

Jeanine Sanders, BS,
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Re: [Histonet] Histonet Digest, Vol 201, Issue 3

[Madeleine Huey via Histonet]

PH 405-759-3953
> http://www.excaliburpathology.com
>
> A sharp knife is nothing without a sharp eye. - Klingon Proverb
>
> On Wednesday, August 5, 2020, 07:57:05 AM CDT, Sanders, Jeanine
> (CDC/DDID/NCEZID/DHCPP) via Histonet 
> wrote:
>
>  Good morning!
>
> I need to provide some information to my team about film vs. glass
> coverslipping. I have a lot of positive info. regarding film but are any
> cons? And I need to come up wit the pros of glass over film. Anyone with
> extended experience have some current information for me?
>
> Thanks very much,
>
> Jeanine Sanders, BS, HT(ASCP), QIHCCM(ASCP)
> Centers for Diseases Control and Prevention
> 1600 Clifton Road NE
> MS H18-SB
> Bldg. 18, Rm SB-114
> Atlanta, GA 30329
> 404-639-3590
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> --
>
> Message: 4
> Date: Wed, 5 Aug 2020 15:22:48 +
> From: "Waitts, Celeste" 
> To: "'histonet@lists.utsouthwestern.edu'"
> 
> Subject: [Histonet] MMR IHC validaton
> Message-ID:
> <8c30fb230afd42f7a54c520887028...@exchnode1.local.centrastate.com>
> Content-Type: text/plain; charset="us-ascii"
>
> HI,
> WHAT is everyone's opinion on the best way to validate the Roche MMR IHC
> panel.  Cost, time, ease?
> Any help?
> Thank you
> Celeste Waitts
> Histology Supervisor
> Centrastate Medical Center
>
>
>
>
> --
>
> Message: 5
> Date: Wed, 5 Aug 2020 15:34:28 +
> From: "Heckford, Karen - SMMC-SF" 
> To: "histonet@lists.utsouthwestern.edu"
> 
> Subject: [Histonet] Cytology
> Message-ID: <903be99abb344593a73079761d762...@phx-exch-013.chw.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> Good Morning,
> I rarely do Special stains or IHC's on Cytology cytospins or thin prep
> type slides.Should these slides be charged, air dried or fixed in 95%
> alcohol before doing Special stains or IHC's on them?
>
> Is there a good book or some sort of publication on cytology procedures
> regarding the above mentioned.   I have tried and look it up and it seems
> all over the place on what to do.
>
> Any help would be greatly appreciated,
>
> Karen Heckford HT ASCP CE
> Lead Histology Technician
> St. Mary's Medical Center
> 450 Stanyan St.
> San Francisco, Ca. 94117
> 415-668-1000 ext. 6167
> karen.heckf...@dignityhealth.org<mailto:karen.heckf...@dignityhealth.org>
>
> Caution:  This email message, including all content and attachments, is
> CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.  The
> information contained in this email message is intended only for the use of
> the recipient(s) named above. If the reader of this message is not the
> intended recipient or an agent responsible for delivering it to the
> intended recipient, you have received this document in error.  Any further
> review, dissemination, distribution, or copying of this message is strictly
> prohibited.  If you have received this communication in error, please
> notify us  immediately by reply email.  Thank you
>
>
>
> --
>
> Message: 6
> Date: Wed, 5 Aug 2020 15:38:14 +
> From: "Morken, Timothy" 
> To: "Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP)" 
> Cc: Histonet 
> Subject: Re: [Histonet] Update info. on old topic?
> Message-ID:
> <
> byapr05mb57039195dfe1e6c67b67b1f7e7...@byapr05mb5703.namprd05.prod.outlook.com
> >
>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi Jeanine!
>
> We are using film coverslipping exclusively now and scanning all slides.
> We moved to film because it dries almost instantly and can be scanned right
> away. It has worked very well. The only issues, and not specific to film
> coverslipping,  are that some slides with low contrast sometimes are out of
> focus. That is primarily IHC slides with low contrast counter stain and low
> contrast specific staining.
>
> Tim Morken
> Supervisor, Electron Microscopy/Neuromuscular Special Studies
> Department of Pathology
> UC San Francisco Medical Center
>
> -Original Message-
> From: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) via Histonet <
> histonet@lists.utsouthwestern.edu>
> Sent: Wednesday, August 05, 2020 5:40 AM
> To: 'histonet@lists.utsouthwestern.edu'  >
> Subject: [Histonet] Update info. on old topic?
>
> Good morning!
>
> I need to provide 

Re: [Histonet] Histonet Digest, Vol 200, Issue 19

[Madeleine Huey via Histonet]

Original Message-
From: Ken M via Histonet 
Sent: Tuesday, July 28, 2020 11:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Apple Green Birefringence in Amyliod slides

Hi Ken,
May I give you my personal experience on Congo Red?
1) Always cut thick tissue (ie,10um thickness, but 8 um will work)
2) Always fresh cut tissue. You can pre-cut control slides, but store them
in the *dark* @ *4C (ie. fridge, never RT)*.
Amyloid will fade if pre-cut and stored at room temperature with light.  We
always stored our stained control slide as reference along with our pre-cut
unstained slides in the fridge, but "apple green birefringence" will also
fade with time (unstable).


*Madeleine Huey, **B.S., HTL & QIHC (ASCP)*

Supervisor, Pathology/Histology & IPOX (MV & LG)

El Camino Hospital

2500 Grant Road (Rm GC-33)

Mountain View, CA 94040

Phone:  650-940-7038

Fax:650-988-8387

Email:  madelein...@elcaminohospital.org


Hi everyone.  I was wondering if anyone out there has any experience with
diagnosing Amyloid tissue using Congo Red stained Kidney using polarized
lenses.  Is it common to use polarized light to detect Amyloid deposits?
Does the absence of the "apple green birefringence" indicate a problem with
the control tissue or the control slides?  Should this green bifringence
always appear to confirm the diagnosis?  I know that the tissue should be
cut thicker than normal (we usually cut at 5), but in the future maybe we
will cut at 7 or 8?

On Thu, Jul 30, 2020 at 10:04 AM 
wrote:

> Send Histonet mailing list submissions to
> histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-requ...@lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-ow...@lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
>1. Re: Apple Green Birefringence in Amyliod slides (John Kiernan)
>2. Sakura Tissue-Tek versus Tanner embedding station Comparison
>   (Rob Rankin)
>
>
> --
>
> Message: 1
> Date: Wed, 29 Jul 2020 18:39:31 +
> From: John Kiernan 
> To: Ken M , "Morken, Timothy"
> 
> Cc: Histonet 
> Subject: Re: [Histonet] Apple Green Birefringence in Amyliod slides
> Message-ID:
> <
> ytopr0101mb161161fc59817a6b556ccdefa5...@ytopr0101mb1611.canprd01.prod.outlook.com
> >
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> For another source of polarizing filters, go to a 3D movie, take home the
> glasses they provide, and poke out the lenses. They work very nicely as
> polarizer and analyzer with an ordinary microscope.
> John Kiernan
> Anatomy & Cell Biology
> University of Western Ontario
> London, Canada
> = = =
> 
> From: Morken, Timothy via Histonet 
> Sent: 28 July 2020 13:57
> To: Ken M 
> Cc: Histonet 
> Subject: Re: [Histonet] Apple Green Birefringence in Amyliod slides
>
> Ken, Yes, polarized light and apple green birefringence is diagnostic for
> amyloid with congo red and is the best practice. If you have a problem with
> known control slides  there are two possibilities: 1) make up fresh
> solution. The pH has to be right. Or 2) try other control slides. Maybe you
> cut through the amyloid area.
>
> Because we have hundreds of microscopes in our department most just use
> polarized film as the polarizer (put over the light source) and another put
> over the top of the slide as the analyzer. Turn one of the polarizing
> slides and you will see the birefringence appear.
>
> Source:
> "Polarizing film, 2"" x 2"" , PK/10 (BEST For use as a microscope
> polarizer)"   Cat# S07372 Thermo Fisher Sci Health$36.75
> PK/10   "2" x 2"
>
> These are polarized film mounted in 2" film holders (like the old
> Kodachrome slides).
>
> Cheap and effective. (and avoids consternation from people losing
> expensive microscope polarizers)
>
> Tim Morken
> Supervisor, Electron Microscopy/Neuromuscular Special Studies
> Department of Pathology
> UC San Francisco Medical Center
>
>
> -Original Message-
> From: Ken M via Histonet 
> Sent: Tuesday, July 28, 2020 11:43 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Apple Green Birefringence in Amyliod slides
>
> Hi everyone.  I was wondering if anyone out there has any experience with
> diagnosing Amyloid tissue using Congo Red stained Kidney using polarized
> lenses.  Is it common to use polarized light to detect Amyloid deposits?
> Does the absence of the "apple green birefringence" indicate a problem with
> the control tissue or the control slides?  Should this green bifringence
> always appear to confirm the diagnosis?  I know that the tissue should be

Re: [Histonet] Histonet Digest, Vol 200, Issue 9

[Ken Urban via Histonet]

Charles Culling is the name/person you’re trying to remember.

Sent from my iPhone

> On Jul 10, 2020, at 12:00 PM, histonet-requ...@lists.utsouthwestern.edu wrote:
> 
> Send Histonet mailing list submissions to
>histonet@lists.utsouthwestern.edu
> 
> To subscribe or unsubscribe via the World Wide Web, visit
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
>histonet-requ...@lists.utsouthwestern.edu
> 
> You can reach the person managing the list at
>histonet-ow...@lists.utsouthwestern.edu
> 
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> 
> 
> Today's Topics:
> 
>   1. Re: Crystals forming in Hematoxylin (Hannen, Valerie)
>   2. Re: Crystals forming in Hematoxylin (Paula Keene Pierce)
>   3. Histology text book  "bibles" (Hobbs, Carl)
>   4. Re: Histology text book  "bibles" (Muhammad Azam)
> 
> 
> --
> 
> Message: 1
> Date: Thu, 9 Jul 2020 17:09:28 +
> From: "Hannen, Valerie" 
> To: 'Terri Braud' 
> Cc: "Histonet@lists.utsouthwestern.edu"
>
> Subject: Re: [Histonet] Crystals forming in Hematoxylin
> Message-ID:
>
> Content-Type: text/plain; charset="us-ascii"
> 
> Hi Terri,
> 
> The crystals form during cold winter months, usually during shipping or can 
> form if the Hematoxylin 7211 is stored in a cold environment.  They also form 
> due to the Mordant being put into solution at it's saturation point.
> The crystals have no adverse effect on the stain itself.  If your bottle has 
> a few crystals, just filter it and it should remain crystal free.  If there 
> are a lot, you can gently warm it, using a "hot" plate and constant stirring 
> until the crystals go back into solution, then filter it and is should be 
> good to go.   I only learned this when I "googled" about it myself.  I hope 
> this helps!!
> 
> Regards,
> 
> Valerie
> 
> 
> Valerie Hannen,MLT(ASCP),HTL,SU (FL)
> Section Chief, Histology
> Parrish Medical Center
> 951 N. Washington Ave.
> Titusville,Florida 32796
> T: (321)268-6333 ext. 7506
> F: (321) 268-6149
> valerie.han...@parrishmed.com
> www.parrishmed.com
> 
> 
> 
> -Original Message-
> From: Terri Braud via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
> Sent: Thursday, July 09, 2020 12:39 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [EXTERNAL Sender] [Histonet] Crystals forming in Hematoxylin
> 
> 
> This message came from an external source. Please do not click links or open 
> attachments if unexpected or unusual.
> 
> Begin Original Message:
> 
> --
> Hello Histo Peeps - What are the clear crystals that form in Hematoxylin?  We 
> use Richard-Allen 7211.  We love the stain, but occasionally, when a bottle 
> has been previously opened, we find clear crystalline precipitate forming in 
> the bottom.  Today, we had a huge one (over an 2" in greatest diameter) that 
> was quite beautiful.  It had a few occlusions of purple streaks, but 
> otherwise was clear.  Doesn't seem to affect the stain, so I was just curious.
> Inquisitively yours, Terri
> 
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!PaPH0eDnAlaiSnq7Yv-U!vBWHyLqdMq6OOl66sorBVdmBIxsRePjH1moP6w3bOijwraARJ_4VQ8f_A8SaZWSs2RVXMg$
>  
> 
> 
> 
> --
> 
> Message: 2
> Date: Thu, 9 Jul 2020 17:21:29 + (UTC)
> From: Paula Keene Pierce 
> To: "histonet@lists.utsouthwestern.edu"
>,Terri Braud
>
> Subject: Re: [Histonet] Crystals forming in Hematoxylin
> Message-ID: <114995151.2633909.1594315289...@mail.yahoo.com>
> Content-Type: text/plain; charset=UTF-8
> 
> That is the aluminum salt precipitating out.
> Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
> Blue Lake DriveNorman, OK 73069PH 
> 405-759-3953http://www.excaliburpathology.com
> 
> A sharp knife is nothing without a sharp eye. - Klingon Proverb 
> 
>On Thursday, July 9, 2020, 11:47:53 AM CDT, Terri Braud via Histonet 
>  wrote:  
> 
> Hello Histo Peeps - What are the clear crystals that form in Hematoxylin?? We 
> use Richard-Allen 7211.? We love the stain, but occasionally, when a bottle 
> has been previously opened, we find clear crystalline precipitate forming in 
> the bottom.? Today, we had a huge one (over an 2" in greatest diameter) that 
> was quite beautiful.? It had a few occlusions of purple streaks, but 
> otherwise was clear.? Doesn't seem to affect the stain, so I was just curious.
> Inquisitively y

Re: [Histonet] Histonet Digest, Vol 200, Issue 5

[Muhammad Azam via Histonet]

I take it u r talking about Prof. Kieran. We r lucky to have access to Prof 
Kiernan’s vast experience and wisdom.

Dr Azam

Sent from my iPhone

> On Jul 8, 2020, at 11:49 AM, King,Michael A via Histonet 
>  wrote:
> 
> Happy to second that opinion, that was probably the single most valuable 
> training and reference text in my career as a research neurobiologist (also 
> hunt down his archived Histonet posts on formaldehyde fixation, essential 
> reading for lab pros). Others apparently agreed, as I had to replace a couple 
> copies that got 'lost' over the years, but that was reason to get the latest 
> editions. It was good to see him still responding here, and hope he will be 
> gladdened to know that dozens of students who passed through my lab came to 
> appreciate and benefit from his terrific approach to the technology. 
> ---
> 5 Jul 2020 19:25:51 +
> From: "Hobbs, Carl" 
> To: "histonet@lists.utsouthwestern.edu"
>
> Subject: Re: [Histonet] Fixed frozen non-paraffin mouse brain
> Message-ID:
>
> 
> 
> Content-Type: text/plain; charset="iso-8859-1"
> 
> Prof. Kiernan, as usual, provides us all with such a depth/breadth of 
> particular information/advice.
> His Histological and Histochemical methods BIBLE is still my favourite read.
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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Re: [Histonet] Histonet Digest, Vol 200, Issue 5

[King,Michael A via Histonet]

Happy to second that opinion, that was probably the single most valuable 
training and reference text in my career as a research neurobiologist (also 
hunt down his archived Histonet posts on formaldehyde fixation, essential 
reading for lab pros). Others apparently agreed, as I had to replace a couple 
copies that got 'lost' over the years, but that was reason to get the latest 
editions. It was good to see him still responding here, and hope he will be 
gladdened to know that dozens of students who passed through my lab came to 
appreciate and benefit from his terrific approach to the technology. 
---
5 Jul 2020 19:25:51 +
From: "Hobbs, Carl" 
To: "histonet@lists.utsouthwestern.edu"

Subject: Re: [Histonet] Fixed frozen non-paraffin mouse brain
Message-ID:



Content-Type: text/plain; charset="iso-8859-1"

Prof. Kiernan, as usual, provides us all with such a depth/breadth of 
particular information/advice.
His Histological and Histochemical methods BIBLE is still my favourite read.
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Re: [Histonet] Histonet Digest, Vol 199, Issue 12

[Dee Neamand via Histonet]

Hi Anne. We’ve been recycling xylene for many years. We purchased our unit 
through CBG biotech. They have a formalin recycling unit and a solvent 
recycling unit. The solvent unit can be used to recycle ethanol, xylene, and 
xylene substitutes. Let me know if you need more info or would like to chat 
about them. 
Dee Neamand, HT (ASCP)
Lead Tech
Evangelical Community Hospital
Lewisburg PA

Sent from my iPhone

> On Jun 23, 2020, at 1:17 PM, histonet-requ...@lists.utsouthwestern.edu wrote:
> 
> Send Histonet mailing list submissions to
>histonet@lists.utsouthwestern.edu
> 
> To subscribe or unsubscribe via the World Wide Web, visit
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
>histonet-requ...@lists.utsouthwestern.edu
> 
> You can reach the person managing the list at
>histonet-ow...@lists.utsouthwestern.edu
> 
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> 
> 
> Today's Topics:
> 
>   1. Xylene recycle (Anne Murvosh)
> 
> 
> --
> 
> Message: 1
> Date: Tue, 23 Jun 2020 14:39:59 +
> From: Anne Murvosh 
> To: "histonet@lists.utsouthwestern.edu"
>
> Subject: [Histonet] Xylene recycle
> Message-ID:
><22bdd9aabc13e24e95d1cf064b75c4b7cb9...@exchange.advancederm.net>
> Content-Type: text/plain; charset="us-ascii"
> 
> I'm looking to switch from a disposal pick-up to recycling of Xylene. Can 
> anyone tell me some companies that offer a recycling unit. The catch is we 
> don't have very much so I need something for a small lab. Thanks Anne
> 
> 
> --
> 
> Subject: Digest Footer
> 
> ___
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> --
> 
> End of Histonet Digest, Vol 199, Issue 12
> *


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Re: [Histonet] Histonet Digest, Vol 199, Issue 9

[Madeleine Huey via Histonet]

Hello,
Does anyone have Covid 19 FFPE tissues or Transient/Stable Cell Line
(slides/block) ?  I have some H.Pylori blocks for exchanges.

*Madeleine Huey, B.S., HTL & QIHC (ASCP)*

Supervisor, Pathology/Histology & IPOX (MV & LG)

El Camino Hospital

2500 Grant Road (Rm GC-33)

Mountain View, CA 94040

Phone:  650-940-7038

Fax:650-988-8387

Email:  madelein...@elcaminohospital.org


On Tue, Jun 16, 2020 at 10:20 AM 
wrote:

> Send Histonet mailing list submissions to
> histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
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>1. COVID 19 Policy (Richardson, Pam K)
>
>
> --
>
> Message: 1
> Date: Mon, 15 Jun 2020 20:19:31 +
> From: "Richardson, Pam K" 
> To: "histonet@lists.utsouthwestern.edu"
> 
> Subject: [Histonet] COVID 19 Policy
> Message-ID:
> <
> byapr10mb25820be19a2d0ed3386b37c6dd...@byapr10mb2582.namprd10.prod.outlook.com
> >
>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi, has anyone created a policy that you are willing to share for the
> handling of fresh specimens from suspect or COVID positive patients?
>
> Pam ~
> National Histology Professionals Day 3/10/20
> Pathologists' Assistant Day 4/14/2020
> Medical Laboratory Professionals Week April 19-25, 2020
> National Cytotechnology Day 5/13/2020
>
> +++
> Pam Richardson
> Clinical Manager
> Gundersen Health System Laboratory Services
> Email: pkric...@gundersenhealth.org
> Phone: 608 775-4133
> Fax: 608 775-6136
> Interdepartmental Mail Stop: H04-007
> E-visit us at: http://www.gundersenhealth.org
>
>
>
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> Subject: Digest Footer
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> End of Histonet Digest, Vol 199, Issue 9
> 
>
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Re: [Histonet] Histonet Digest, Vol 198, Issue 1

[King,Michael A via Histonet]

Make sure sodium azide (antimicrobial preservative) is not used in any of your 
IHC reagents--it inhibits peroxidase.
Mike

---
Message: 3
Date: Fri, 1 May 2020 08:00:49 -0700
From: Kristy Castillo 
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC DAB
Message-ID:

Content-Type: text/plain; charset="UTF-8"

Hi Histonetters,

We are starting our IHC (fun times), we are having trouble with the DAB
lighting up.  Processed for 5 thru 10 minutes and still nothing.  Our
permanent red is working just fine.  Any ideas.

Thank you!

Kristy


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Re: [Histonet] Histonet Digest, Vol 197, Issue 23 recovering RNA from FFPE

[Paula Keene Pierce via Histonet]

Biochain too.
Paula
 

On Saturday, April 25, 2020, 01:08:53 PM CDT, Steve McClain via Histonet 
 wrote:  
 
 1) Does anyone know of a method of recovering RNA from FFPE?
2) Does anyone know a lab that has done it?

Thanks,
Steve A. McClain, MD
McClain Labs
45 Manor Road Smithtown, NY 11787
631 361-4000 cell 631 926-3655

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Re: [Histonet] Histonet Digest, Vol 197, Issue 23 recovering RNA from FFPE

[Paula Keene Pierce via Histonet]

By doing an internet search for FFPE PCR Platform, Thermo, Agilent, Biogen, 
Qiagen, etc. all have this.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Saturday, April 25, 2020, 01:08:53 PM CDT, Steve McClain via Histonet 
 wrote:  
 
 1) Does anyone know of a method of recovering RNA from FFPE?
2) Does anyone know a lab that has done it?

Thanks,
Steve A. McClain, MD
McClain Labs
45 Manor Road Smithtown, NY 11787
631 361-4000 cell 631 926-3655

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Re: [Histonet] Histonet Digest, Vol 197, Issue 23 recovering RNA from FFPE

[Steve McClain via Histonet]

1) Does anyone know of a method of recovering RNA from FFPE?
2) Does anyone know a lab that has done it?

Thanks,
Steve A. McClain, MD
McClain Labs
45 Manor Road Smithtown, NY 11787
631 361-4000 cell 631 926-3655

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Re: [Histonet] Histonet Digest, Vol 197, New Topic Coronavirus testing from tissue sample

[Steve McClain via Histonet]

The CDC website with specimen submission form does not seem to be working.
https://www.cdc.gov/laboratory/specimen-submission/pdf/form-50-34.pdf

Does anyone know who can else can test for Corona virus from FFPE tissue?
I have an unusual skin biopsy from late December (earlier than the first known 
US case)

Thanks,
Steve A. McClain, MD
McClain Labs
45 Manor Road Smithtown, NY 11787
631 361-4000 cell 631 926-3655

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Re: [Histonet] Histonet Digest, Vol 197, Issue 8

[John Garratt via Histonet]

Good morning, please take a look at one of the EQA reports (example in the link 
below) for MMR from the Canadian Pathology Quality Assurance programme. 
(www.cpqa.ca) .
You cannot say MMR is positive or negative since it is confusing but say if 
there is Expression or Absence of Expression of a MMR protein. Fortunately 
tissues come with their own internal "positive" and "negative" controls which 
you can utilise. You could design a verification program around the presence or 
absence of a particular MMR protein should you wish.

http://cpqa.ca/assessments/Run%2096%20MMR%20Summary.pdf

Should you wish to receive our technically useful EQA reports in your mailbox 
email subscripti...@cpqa.ca putting SUBSCRIBE in the subject box.

John


www.cpqa.ca

‐‐‐ Original Message ‐‐‐
On Tuesday, April 14, 2020 6:54 AM, Dionee Neamand via Histonet 
 wrote:

> Hello. I am looking for help with IHC staining. Does anyone have good 
> negative control tissue for MMR stains? We need a minimum of 10 negative 
> results per IHC stain for our validation. We have yet to find any. Any 
> suggestions would be helpful.
> Thanks,Dee
> Dionee Neamand, HT (ASCP)Lead TechEvangelical Community HospitalLewisburg  PA 
>  17837
>
> -Original Message-
> To: histonet@lists.utsouthwestern.edu
> Sent: Thu, Apr 9, 2020 1:00 pm
> Subject: Histonet Digest, Vol 197, Issue 8
>
> Send Histonet mailing list submissions to
>     histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
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>
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>     histonet-ow...@lists.utsouthwestern.edu
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> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
> Today's Topics:
>
>   1. Question concerning H-Pylori Staining (Ken M)
>   2. Is there anything I can do for you? (Pam Barker)
>   3. Competency questionnaires (Charles Riley)
>
>
> --
>
> Message: 1
> Date: Wed, 8 Apr 2020 19:50:09 +
> From: Ken M kdea...@hotmail.com
> To: Histo List histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Question concerning H-Pylori Staining
> Message-ID:
>     
> sn6pr06mb43343f3f0c62cba60bde54c8ad...@sn6pr06mb4334.namprd06.prod.outlook.com
>
>    
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hi everyone!  We have a question about staining for H-Pylori Using Quick 
> Stain (Periodic acid 1%, Alician Yellow, Sodium Metabisulfate,Toluidine Blue 
> stock, Sodium Hydroxide) we notice what clearly  looks like the H-Pylori 
> purple stained clusters, but after dehydration in 100% alcohol the purple 
> clusters seem to disappear. Should we just dehydrate using a slide oven 
> instead of the alcohol? For how long at what temp? Could the alcohol be 
> affecting the purple color making it too light to see?
>
> Michelle
>
>
> -------
>
> Message: 2
> Date: Thu, 9 A

Re: [Histonet] Histonet Digest, Vol 197, Issue 8

[Dionee Neamand via Histonet]

Hello. I am looking for help with IHC staining. Does anyone have good negative 
control tissue for MMR stains? We need a minimum of 10 negative results per IHC 
stain for our validation. We have yet to find any. Any suggestions would be 
helpful.
Thanks,Dee
Dionee Neamand, HT (ASCP)Lead TechEvangelical Community HospitalLewisburg  PA  
17837


-Original Message-
To: histonet@lists.utsouthwestern.edu
Sent: Thu, Apr 9, 2020 1:00 pm
Subject: Histonet Digest, Vol 197, Issue 8

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    histonet@lists.utsouthwestern.edu

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When replying, please edit your Subject line so it is more specific
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Today's Topics:

  1. Question concerning H-Pylori Staining (Ken M)
  2. Is there anything I can do for you? (Pam Barker)
  3. Competency questionnaires (Charles Riley)


--

Message: 1
Date: Wed, 8 Apr 2020 19:50:09 +
From: Ken M 
To: Histo List 
Subject: [Histonet] Question concerning H-Pylori Staining
Message-ID:
    

    
Content-Type: text/plain; charset="iso-8859-1"

Hi everyone!  We have a question about staining for H-Pylori Using Quick Stain 
(Periodic acid 1%, Alician Yellow, Sodium Metabisulfate,Toluidine Blue stock, 
Sodium Hydroxide) we notice what clearly  looks like the H-Pylori purple 
stained clusters, but after dehydration in 100% alcohol the purple clusters 
seem to disappear. Should we just dehydrate using a slide oven instead of the 
alcohol? For how long at what temp? Could the alcohol be affecting the purple 
color making it too light to see?

Michelle


--

Message: 2
Date: Thu, 9 Apr 2020 11:15:47 -0400
From: "Pam Barker" 
To: "Histopeeps Histonet" 
Subject: [Histonet] Is there anything I can do for you?
Message-ID: <008901d60e81$bf898880$3e9c9980$@earthlink.net>
Content-Type: text/plain;    charset="iso-8859-1"

Hi Histonetters,
I hope you and your loved ones are staying safe and healthy, and you can
take time to enjoy Easter, Passover  and this season with your family.
It seems that things are changing on an almost daily basis.  
I have been monitoring social media - personal connections and histology
related groups on Facebook and LinkedIN and have been staying in touch with
many of my active candidates and customers.
I have gotten a lot of questions about what is going on with the ?career?
aspect of histology at this time.
Here is a summary of what I have observed and experienced so far:

Histotechs supporting ?non-essential surgeries? i.e. Derm and GI private
practices:
? Some are experiencing cuts in hours and/or are using the time to catch up
on backlogs and lab maintenance.
? Many are being furloughed with the expectation of being called back to
work once ?the all clear is given?,
? Start dates for new employees are being postponed until further notice.

Traveling Histotechs - Histotechs that are working as contractors
nationwide:
? Most of the travel techs I have heard from have been grounded meaning sent
home until further notice.
? They seem confident that they will be returning to their assignments as
they are looking for prn positions for now.

Hospital based histotechs:
? Some are being furloughed
? Some are having their hours reduced
? Some are job sharing meaning for example if they have a regular staff of 4
techs they take turns 2 at a time working for a week while the other 2 stay
home
? Some are being used in other areas for example if they have experience in
phlebotomy they are moving to that department.

I can?t really speak to research, academic or pharmaceutical areas as I
haven?t heard much from them.  (Hopefully that?s because they are busy
working on the COVID-19 response.)

What I glean from this and bear in mind this only an opinion on my part
based on what I have heard anecdotally.  It seems that the best case
scenario at this point is there was a shortage of histotechs before the
pandemic and there will be just as great a need after the pandemic in other
words we are just pressing pause.



Just Remember?
Things Will Get Better.  
This Will End. 
&
We Will Return To Our Normal Lives.

In the meantime?
Is there anything I can do for you?
Were you actively looking for a new position before this crisis?
Are you someone who is unemployed as opposed to furloughed?
Are you feeling lonely or isolated and just need to hear a friendly voice?
Call Me, 
Text Me,
Email Me,
Or Contact Me through Social Media.

We are all in this together?

Looking forward to the light at the end of the tunnel.

Thanks-Pam

Right Time, Right Place, Right Move with RELIA!
*15 Years!*
Celebrating 15 years o

Re: [Histonet] Histonet Digest, Vol 197, Issue 11

[Izak Dimenstein via Histonet]

Please, go to my website grossing-technology.com. In the menu go to COVID-19. 
Open the PPE and beyond post. In the end of it  is you. Any suggestions for 
corrections. Thank you.

Sent from my iPad

> On Apr 13, 2020, at 13:00, "histonet-requ...@lists.utsouthwestern.edu" 
>  wrote:
> 
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> 
>   1. Re: Question concerning H. pylori staining (jkier...@uwo.ca)
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Re: [Histonet] Histonet Digest, Vol 196, Issue 3 Incomplete sectioning

[Steve McClain via Histonet]

message is not the intended 
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--

Message: 2
Date: Tue, 3 Mar 2020 20:46:17 +
From: "Terri  Braud" 
To: "'histonet@lists.utsouthwestern.edu'"

Subject: Re: [Histonet] Incomplete sectioning
Message-ID:

<48E053DDF6CE074DB6A7414BA05403F801C1B77C52@HRHEX02-HOS.holyredeemer.local>

Content-Type: text/plain; charset="us-ascii"

A quality check can be accomplished in 2 places.  It can be done at cutting, 
but it should already be being done and it doesn't seem to be working.
Ideally, the stained H&E should be checked against the block face as it is 
pulled from the coverslipper to be given to the pathologist. Then it can be 
handed immediately to the tech that made the original error to  "do over"
Also, a common excuse will be "it was embedded poorly".  The answer to that is 
that it is the cutting tech's responsibility to hand back a poorly embedded 
block for it to be re-embedded if they feel that they can't get a 
representation section.
Remember, it could also be poorly cut gross, too.
Then you can nip it in the bud before the slide reaches the pathologist, and 
you can quickly identify who is turning out inferior sections and counsel them 
appropriately if needed.
Hope this helps. Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal


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[mailto:histonet-requ...@lists.utsouthwestern.edu] 
Sent: Tuesday, March 03, 2020 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 196, Issue 2

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Today's Topics:

   1. Incomplete cross sections of all tissue in blocks (Amy Self)
   2. Re: Incomplete cross sections of all tissue in blocks
  (John Garratt)


--

Message: 1
Date: Tue, 3 Mar 2020 15:31:59 +
From: Amy Self 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] Incomplete cross sections of all tissue in blocks
Message-ID:



Content-Type: text/plain; charset="utf-8"

Good Morning HistoNetters,

I am reaching out to the histonet in hopes to get some suggestions from you on 
how to handle incomplete cross-sections of tissue in blocks. We are a small lab 
so this has not been an issue in the past but now that we are growing and our 
staff has increased I am getting feed-back from pathologist that the sections 
of tissue are not complete. They are asking for too many deepers that possibly 
could be avoided if it was cut deep enough to begin with. I have been given 
some managerial type duties ? which I don?t like cause I know nothing about 
managing people and I need to approach this but I need to approach this issue 
correctly.  Do you have the histotech compare his/her cut slides to the block 
to make sure that a complete cross-section is obtained and is this documented 
somehow?  Any and all suggestions I need.

Thanks in advance for your help and as always you all rock.. ?

Amy Self
Histology Lab Senior Tech
Lab
Tidelands Georgetown Memorial Hospital
606 Black River Road
Georgetown, SC 29440
(843) 520-8711
as...@tidelandshealth.org
Our mission:  We help people live better lives through better health.


NOTE:
The information contained in this message may be privileged, confidential and 
protected from disclosure. If the reader of this messa

Re: [Histonet] Histonet Digest, Vol 196, Issue 3

[NWL - Histology via Histonet]

Re: Histonet digest Incomplete sectioning of blocks.

I had to manage this situation a few year back and found that the best place to 
capture this is at the waterbath. QC reviews I.e numbers of return slides 
categorised into fault type can be collated over a month and displayed easily 
to a team of people. This data can also be a useful KPI of your labs processes. 
You can then use as evidence if you had to go down the route of a capability 
process with a single member of staff.

Do you microscopically check each slide that is sent out?

Cheers
Stuart Beaver

Head of Histology
Nationwide Laboratories
Poulton-le-Fylde
UK

Get Outlook for iOS

From: histonet-requ...@lists.utsouthwestern.edu 

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To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 196, Issue 3

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Re: [Histonet] Histonet Digest, Vol 195, Issue 10

[White, Marcia via Histonet]

We do not assist

 but do go in and perform a frozen section, if this is the assistance they are 
giving the pathologist then this is normal


Marcia White
Director Memorial Regional Pathology Services
9581  Premier Parkway, Miramar FL 33025
(954) 276-1855 | Fax: 954-967-7627
 
 

-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 
[mailto:histonet-requ...@lists.utsouthwestern.edu] 
Sent: Thursday, February 13, 2020 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 195, Issue 10

 

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Re: [Histonet] Histonet Digest, Vol 195, Issue 10

[Patsy Ruegg via Histonet]

I never minded being called in to assist for an aspect of transplants at the U, 
I figured I was helping a very needy patient.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com


From: histonet-requ...@lists.utsouthwestern.edu 

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Subject: Histonet Digest, Vol 195, Issue 10

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Re: [Histonet] Histonet Digest, Vol 195, Issue 6

[Karim Ruknuddin via Histonet]

Dear All,
I am planning to introduce RNAscope in my pathology core facility, we are 
currently gathering information on both chromogen detection and florescent 
detection, but the company we are looking into for reagent ordering which is 
ACDbio has a wide range of channel 1  and channel 2 probes of human species, 
but limited channel 3 and channel 4 probes, they say you can even custom  
design your probes but they are expensive.

Can anyone refer What detection system are you using or would recommend and why?

Regards
Karim
Research Associate
Aga Khan University Hospital
Karachi

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Today's Topics:

   1. Re: How to Reduce Tissue Autofluorescence (Hobbs, Carl)
   2. Re: How to Reduce Tissue Autofluorescence (John Kiernan)


--

Message: 1
Date: Sat, 8 Feb 2020 18:56:09 +
From: "Hobbs, Carl" 
To: histonet 
Subject: Re: [Histonet] How to Reduce Tissue Autofluorescence
Message-ID:


Content-Type: text/plain; charset="iso-8859-1"

Hi
Do you refer to FIF?
Or...autofluorescence?
Different...as you prob know so, apologies in advance.
The Wright Cell imaging Facility ( Toronto western research Inst) pdf: very 
informative
FIF: I have tried Glycine, Ammonium chloride and Na-borohyd.
I got best results with Glycine
Howevernone are great.
Multi spectral imaging is the best way forwards butexpensive.
Sure, in Lambda mode using Zeiss Zen on a confocal gives good results ( tho I 
have forgotten how to do it, sigh) After setting up you hit the fluorescence 
you don't want...hit the one you wantif the wavelengths are different, what 
you don't want you eliminate, electronically.
Vectorlabs sell an excellent kit for FIF elimination...sure over-expensive, 
given the ingredients.
We found that dilution of their working reagent by x2- x4 gave best results As 
good as multispectral imaging, imho.
Autofl of lipofuscin is supressed using Sudan BlackB.

Good luck




Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge?
Kings College London
London
SE1 1UL
?

020 7848 6813


--

Message: 2
Date: Sun, 9 Feb 2020 05:55:27 +
From: John Kiernan 
To: "histonet@lists.utsouthwestern.edu"
,Arun Jyothi S.P

Subject: Re: [Histonet] How to Reduce Tissue Autofluorescence
Message-ID:


Content-Type: text/plain; charset="Windows-1252"

There's a very brief article (downloadable PDF) from 2002 about suppressing 
autofluorescence, with a few references, at 
https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.researchgate.net%2Fpublication%2F10971457_Suppressing_autofluorescence&data=02%7C01%7Ckarim.ruknuddin%40aku.edu%7C9a6dffb091094523889608d7ad8a0353%7Ca5d4252a02f94e6096f09733baae4919%7C0%7C0%7C637168680646861537&sdata=gT64wOQByicxQn6OF9%2Fnu4A8rylTvcWFfYul736i7kA%3D&reserved=0
[https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fi1.rgstatic.net%2Fpublication%2F10971457_Suppressing_autofluorescence%2Flinks%2F00463520275ec6a5f100%2Flargepreview.png&data=02%7C01%7Ckarim.ruknuddin%40aku.edu%7C9a6dffb091094523889608d7ad8a0353%7Ca5d4252a02f94e6096f09733baae4919%7C0%7C0%7C637168680646861537&sdata=0sxLU%2BhhEA75T3Wz5c5kSBDbmQPsICiOXjrw2vQNEvc%3D&reserved=0]
(PDF) Suppressing autofluorescence - 
ResearchGate

Re: [Histonet] Histonet Digest, Vol 195, Issue 1

[LEROY H BROWN via Histonet]

Hi,  I am looking for a decent Gram +/- control tissue.   Would be happy to
exchange another type of control in exchange for a good block of tissue for
this.   I have great PAS or Warthan Starry or ?  others too.Can someone
help with this?
Thanks. 
LeRoy Brown HT(ASCP) HTL
1...@comcast.net is my email
Thanks


-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu
 
Sent: Tuesday, February 4, 2020 10:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 195, Issue 1

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Re: [Histonet] Histonet Digest, Vol 194, Issue 15

[Betsy Molinari via Histonet]

No I did not. But I will keep that in mind if it happens again. Thanks!


Betsy Molinari, HT (ASCP)
Sr. Histology Research Technician
CV Pathology Research

Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812 
Email: bmolin...@texasheart.org
texasheart.org  | facebook 
 | twitter 
-Original Message-
From: Rathborne, Toni  
Sent: Tuesday, January 21, 2020 8:56 AM
To: Betsy Molinari 
Subject: RE: [Histonet] Histonet Digest, Vol 194, Issue 15

*** Important*** This email is not from Texas Heart Institute. Only click links 
or open attachments you know are safe.



Betsy,

I realize that they have been stained now, but did you considered running them 
back through alcohols and xylene and then coverslipping? The cells would then 
be preserved and you could easily remove the coverglass and stain at a later 
date.

Toni




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-Original Message-
From: Betsy Molinari via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, January 21, 2020 7:53 AM
To: Charles Riley; 'Histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Histonet Digest, Vol 194, Issue 15


*** This is an External Email ***

Charles, thank you for your reply. That is an interesting idea. I think I may 
have a bit of an experiment to run when I have some time.


Betsy Molinari, HT (ASCP)
Sr. Histology Research Technician
CV Pathology Research

Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812
Email: bmolin...@texasheart.org
texasheart.org<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.texasheart.org_&d=DwICAg&c=LfJFs5tz11XIvZ1zGnYRWYcpprcdQWHKbyr0OjT-Gjk&r=OywojvDeqnDOvbIWXIx1jW-8xZXD1RJBnKKp8Mh6i_g&m=p0Bu9RgcZAx01hkG1tJGDgaadnPqItg-8ntFXtGIGqk&s=s7JcJGstAwJMFQxH2cwi5datQ8koH8e3jHm8y1UbT_4&e=
 > | 
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 > | 
twitter<https://urldefense.proofpoint.com/v2/url?u=https-3A__twitter.com_Texas-5FHeart&d=DwICAg&c=LfJFs5tz11XIvZ1zGnYRWYcpprcdQWHKbyr0OjT-Gjk&r=OywojvDeqnDOvbIWXIx1jW-8xZXD1RJBnKKp8Mh6i_g&m=p0Bu9RgcZAx01hkG1tJGDgaadnPqItg-8ntFXtGIGqk&s=ThGMpUv5CaIuL3kg99fGUkfvCgGe7CyppXv-cT0AFXo&e=
 >
From: Charles Riley 
Sent: Tuesday, January 21, 2020 5:55 AM
To: Betsy Molinari 
Subject: Re: [Histonet] Histonet Digest, Vol 194, Issue 15

*** Important*** This email is not from Texas Heart Institute. Only click links 
or open attachments you know are safe.


I have never done this before but I would assume you could reseal it just like 
you would a paraffin block by putting some fresh paraffin on top of the section 
again and allowing it to dry. It would just then need to be reheated and run 
down to remove the paraffin cover before staining.

On Tue, Jan 21, 2020 at 6:50 AM Betsy Molinari via Histonet 
mailto:histonet@lists.utsouthwestern.edu>> 
wrote:
Thank you for the reply Amos. I totally agree but the researcher did not inform 
me until after the deparaffinization was finished. She wanted to hold off on 
the staining but it was too late and I went ahead with the staining. I was just 
curious if there was, in fact a procedure to preserve a deparaffinized slide.


Betsy Molinari, HT (ASCP)
Sr. Histology Research Technician
CV Pathology Research

Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812
Email: bmolin...@texasheart.org<mailto:bmolin...@texasheart.org>
texasheart.org<https://urldefense.proofpoint.com/v2/url?u=http-3A__texasheart.org&d=DwICAg&c=LfJFs5tz11XIvZ1zGnYRWYcpprcdQWHKbyr0OjT-Gjk&r=OywojvDeqnDOvbIWXIx1jW-8xZXD1RJBnKKp8Mh6i_g&m=p0Bu9RgcZAx01hkG1tJGDgaadnPqItg-8ntFXtGIGqk&s=OFPer3U_R8zdgxyhat_LPh27aCF-I77Fd8s90MKKhCY&e=
 > 
<http://www.texasheart.org<https://urldefense.proofpoint.com/v2/url?u=http-3A__www.texasheart.org&d=DwICAg&c=LfJFs5tz11XIvZ1zGnYRWYcpprcdQWHKbyr0OjT-Gjk&r=O

Re: [Histonet] Histonet Digest, Vol 194, Issue 15

[Betsy Molinari via Histonet]

Charles, thank you for your reply. That is an interesting idea. I think I may 
have a bit of an experiment to run when I have some time.


Betsy Molinari, HT (ASCP)
Sr. Histology Research Technician
CV Pathology Research

Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812
Email: bmolin...@texasheart.org
texasheart.org<https://www.texasheart.org/> | 
facebook<https://www.facebook.com/Texas.Heart.Institute> | 
twitter<https://twitter.com/Texas_Heart>
From: Charles Riley 
Sent: Tuesday, January 21, 2020 5:55 AM
To: Betsy Molinari 
Subject: Re: [Histonet] Histonet Digest, Vol 194, Issue 15

*** Important*** This email is not from Texas Heart Institute. Only click links 
or open attachments you know are safe.


I have never done this before but I would assume you could reseal it just like 
you would a paraffin block by putting some fresh paraffin on top of the section 
again and allowing it to dry. It would just then need to be reheated and run 
down to remove the paraffin cover before staining.

On Tue, Jan 21, 2020 at 6:50 AM Betsy Molinari via Histonet 
mailto:histonet@lists.utsouthwestern.edu>> 
wrote:
Thank you for the reply Amos. I totally agree but the researcher did not inform 
me until after the deparaffinization was finished. She wanted to hold off on 
the staining but it was too late and I went ahead with the staining. I was just 
curious if there was, in fact a procedure to preserve a deparaffinized slide.


Betsy Molinari, HT (ASCP)
Sr. Histology Research Technician
CV Pathology Research

Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812
Email: bmolin...@texasheart.org<mailto:bmolin...@texasheart.org>
texasheart.org<http://texasheart.org> 
http://www.texasheart.org>> | facebook 
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 | twitter 
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-Original Message-
From: Amos Brooks via Histonet 
mailto:histonet@lists.utsouthwestern.edu>>
Sent: Monday, January 20, 2020 5:59 PM
To: histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Histonet Digest, Vol 194, Issue 15

*** Important*** This email is not from Texas Heart Institute. Only click links 
or open attachments you know are safe.



Hi Betsy,
 I wouldn't do it. It's an unnecessary risk to let them dry out. Better to 
leave it in distilled water if you absolutely must. Ideal to not deparaffinize 
in the first place if you can't finish the stain. Dried out sections is just a 
bad plan.

Amos Brooks

On Mon, Jan 20, 2020, 1:00 PM 
> Message: 1
> Date: Mon, 20 Jan 2020 12:56:20 +
> From: Betsy Molinari 
> mailto:bmolin...@texasheart.org>>
> To: 
> "'Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu>'"
> 
> mailto:Histonet@lists.utsouthwestern.edu>>
> Subject: [Histonet] Deparaffinized slides
> Message-ID:
> <
> sn6pr10mb28955c0f9dcd3fe5c5fdf3fdce...@sn6pr10mb2895.namprd10.prod.out<mailto:sn6pr10mb28955c0f9dcd3fe5c5fdf3fdce...@sn6pr10mb2895.namprd10.prod.out>
> look.com<https://urldefense.proofpoint.com/v2/url?u=http-3A__look.com&d=DwMFaQ&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=5wwoBF-rqq3qoavhQKMOdIzTCUln4iHFsjxxPGR9Bfo&s=A65vpkkkXUawRC83YPizUWdZcJI2-rmtq1qxbdZHobM&e=>
> >
>
> Content-Type: text/plain; charset="us-ascii"
>
> Good day and Happy Martin Luther King Day. What are your thoughts on
> letting deparaffinized slides dry to be used for future use , or keep
> in DI water for a time? I deparaffinized  some slides this morning,
> then the researcher called and asked me to hold off on staining them.
> I am going to continue staining them after explaining the situation to
> her. But I am just curious if they can be held after being deparaffinized.
> Thank you.
>
> Betsy Molinari HT(ASCP)
> Texas Heart Institute
> Cardiovascular Pathology
> Houston, Texas
>
*
>
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Re: [Histonet] Histonet Digest, Vol 194, Issue 15

[Betsy Molinari via Histonet]

Thank you for the reply Amos. I totally agree but the researcher did not inform 
me until after the deparaffinization was finished. She wanted to hold off on 
the staining but it was too late and I went ahead with the staining. I was just 
curious if there was, in fact a procedure to preserve a deparaffinized slide.  


Betsy Molinari, HT (ASCP)
Sr. Histology Research Technician
CV Pathology Research

Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812 
Email: bmolin...@texasheart.org
texasheart.org  | facebook 
 | twitter 
-Original Message-
From: Amos Brooks via Histonet  
Sent: Monday, January 20, 2020 5:59 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Histonet Digest, Vol 194, Issue 15

*** Important*** This email is not from Texas Heart Institute. Only click links 
or open attachments you know are safe.



Hi Betsy,
 I wouldn't do it. It's an unnecessary risk to let them dry out. Better to 
leave it in distilled water if you absolutely must. Ideal to not deparaffinize 
in the first place if you can't finish the stain. Dried out sections is just a 
bad plan.

Amos Brooks

On Mon, Jan 20, 2020, 1:00 PM 
> Message: 1
> Date: Mon, 20 Jan 2020 12:56:20 +
> From: Betsy Molinari 
> To: "'Histonet@lists.utsouthwestern.edu'"
> 
> Subject: [Histonet] Deparaffinized slides
> Message-ID:
> <
> sn6pr10mb28955c0f9dcd3fe5c5fdf3fdce...@sn6pr10mb2895.namprd10.prod.out
> look.com
> >
>
> Content-Type: text/plain; charset="us-ascii"
>
> Good day and Happy Martin Luther King Day. What are your thoughts on 
> letting deparaffinized slides dry to be used for future use , or keep 
> in DI water for a time? I deparaffinized  some slides this morning, 
> then the researcher called and asked me to hold off on staining them. 
> I am going to continue staining them after explaining the situation to 
> her. But I am just curious if they can be held after being deparaffinized.
> Thank you.
>
> Betsy Molinari HT(ASCP)
> Texas Heart Institute
> Cardiovascular Pathology
> Houston, Texas
>
*
>
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Re: [Histonet] Histonet Digest, Vol 194, Issue 15

[Amos Brooks via Histonet]

Hi Betsy,
 I wouldn't do it. It's an unnecessary risk to let them dry out. Better
to leave it in distilled water if you absolutely must. Ideal to not
deparaffinize in the first place if you can't finish the stain. Dried out
sections is just a bad plan.

Amos Brooks

On Mon, Jan 20, 2020, 1:00 PM 
> Message: 1
> Date: Mon, 20 Jan 2020 12:56:20 +
> From: Betsy Molinari 
> To: "'Histonet@lists.utsouthwestern.edu'"
> 
> Subject: [Histonet] Deparaffinized slides
> Message-ID:
> <
> sn6pr10mb28955c0f9dcd3fe5c5fdf3fdce...@sn6pr10mb2895.namprd10.prod.outlook.com
> >
>
> Content-Type: text/plain; charset="us-ascii"
>
> Good day and Happy Martin Luther King Day. What are your thoughts on
> letting deparaffinized slides dry to be used for future use , or keep in DI
> water for a time? I deparaffinized  some slides this morning, then the
> researcher called and asked me to hold off on staining them. I am going to
> continue staining them after explaining the situation to her. But I am just
> curious if they can be held after being deparaffinized.
> Thank you.
>
> Betsy Molinari HT(ASCP)
> Texas Heart Institute
> Cardiovascular Pathology
> Houston, Texas
>
*
>
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Re: [Histonet] Histonet Digest, Vol 194, Issue 12btattoo removal

[Steve McClain via Histonet]

I don not know any method for tattoo pigment removal.  Aside from carbon black, 
most of the red, yellow, blue and green pigments are metal salts and polarize 
brightly.  The inflammatory response may be vigorous, especially w repeat or 
Re-do of a tattoo. In some cases a pseudo-carcinoma or KA may result.

I am puzzled to learn why the request!  Can anyone in the group think of a 
reason why or purpose for the pathologist needs to request removal?Perhaps 
Staining of infectious organism?

Steve
Steve A. McClain, MD
631-361-4000  Cell 631-926-3655

Hello fellow histonetters,

We received a skin sample that has ink from a tattoo - the sample if from
tattooed skin. One of our pathologists would like us to see if we can get
rid of the tattoo ink from the sections before H&E staining. Does anyone
out there know how to do this?

Thank you,
Donna Emge
Anatomic Pathology Manager
Mercy Hospital and Medical Center, Chicago

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Re: [Histonet] Histonet Digest, Vol 194, Issue 10

[LEROY H BROWN via Histonet]

How do I sign up one of my employees to be given NSH membership.  What is
the cost? 
Thanks,
LeRoy Brown HT(ASCP) HTL

-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu
 
Sent: Wednesday, January 15, 2020 10:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 194, Issue 10

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Re: [Histonet] Histonet Digest, Vol 194, Issue 7

[LEROY H BROWN via Histonet]

I am looking for a small sample of normal stomach for my IHC stain control.
I am willing to trade any of my control blocks if you have a block you can
spare.
Thanks,
LeRoy Brown HT(ASCP) HTL


-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu
 
Sent: Sunday, January 12, 2020 10:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 194, Issue 7

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Re: [Histonet] Histonet Digest, Vol 194, Issue 2

[Olszewski, Dawn via Histonet]

Charles,

We use the same protocol but we also cut everything at 5 microns.


Dawn Olszewski HTL(ASCP)QIHC

Pathology Manager

South Georgia Medical Center

P: (229) 259-4830

E: dawn.olszew...@sgmc.org

Dawn Olszewski
Pathology Manager - Histotechnologist
Laboratory

[cid:MasterLogo_190x61_362365a5-c941-4f16-9f99-9484fd6a6078.png]

South Georgia Medical Center
2501 N. Patterson St.
Valdosta, GA 31602
229-259-4830 (O) | 229-560-6191 (M)
dawn.olszew...@sgmc.org | sgmc.org

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To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 194, Issue 2

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Today's Topics:

   1. H&E protocols (Charles Riley)


--

Message: 1
Date: Fri, 3 Jan 2020 12:42:40 -0500
From: Charles Riley 
To: Histo List 
Subject: [Histonet] H&E protocols
Message-ID:

Content-Type: text/plain; charset="UTF-8"

Does everyone use the same protocol for routine H&E's as well as things
like Cell blocks and or lymph nodes?

If you use a different protocol how does it differ from your routine stain?

--

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs


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Re: [Histonet] Histonet Digest, Vol 192, Issue 4

[Muhammad Azam via Histonet]

Unsubscribe at the bottom 

Sent from my iPhone

> On Nov 6, 2019, at 1:26 PM, Samira Seif via Histonet 
>  wrote:
> 
> To whom it may concern,
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Re: [Histonet] Histonet Digest, Vol 192, Issue 4

[Samira Seif via Histonet]

To whom it may concern,
I would like to stop getting email from histonet.
I was wondering if you let me know how can I do that.
Regards.
Samira


Sent from Mail for Windows 10


From: histonet-requ...@lists.utsouthwestern.edu 

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Re: [Histonet] Histonet Digest, Vol 191, Issue 12

[Subash Govender via Histonet]

Re: How to prevent bubbling in pressure cooker for the immunohistichemistry 
procedure

Hi there Carl

Thank you so much for your advice. I do actually use Marienfeld Histobond  
slides, they are silane coated. So dont think that is the prolem, but i do like 
your advice of drying the tissue under the fan. The tissues that give me 
problems are breast, cervix, and other odd tissues. With breast tissue being 
fatty i suppose you would have a bit of lifting. Another kind lady also gave me 
the idea of actually placing the slides into the pressure cooker and sealing 
the lid immediately after being dewaxed instead of waiting for the solution to 
boil first like i do.
Thanks again for your feedback Carl, much appreciated.
Subash

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From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Saturday, October 19, 2019 7:00:01 PM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 191, Issue 12

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Today's Topics:

1. Re: How to prevent bubbling in pressure cooker for
immunohistochemistry procedure (Hobbs, Carl)
2. Re: Histonet Digest, Vol 191, Issue 10 (Subash Govender)
3. Re: Histonet Digest, Vol 191, Issue 11 (Subash Govender)


--

Message: 1
Date: Fri, 18 Oct 2019 17:58:12 +
From: "Hobbs, Carl" 
To: histonet 
Subject: Re: [Histonet] How to prevent bubbling in pressure cooker for
immunohistochemistry procedure
Message-ID:


Content-Type: text/plain; charset="iso-8859-1"

Hi Subash
Imho your sections are not sufficiently adherent to the slide(s)
It is not M/W "bubbling" that causes your problem
If you use Superfrost Plus/lab-coated Silane or even double subbed slides ( 
thanks Ole!) AND dry your sections onto the slides efficiently, you will not 
get section-loss.
NB: SF+ are expensive: if you cannot afford themexperiment with in-house 
Silane ( APES) or subbing, to coat your IHC slides.
If you use the latter methods, make sure you slides are very clean.
Unless the tissue is very fibrous/bone/cartilage/severely inflameddifficult 
to keep sections adherent.
In the latter case, Trajan 3 series can help much more than std coated slides.
What tissues are causing you problems?
For most soft tissues I use Superfrost Plus slides, dry the mounted sections 
under a fan O/N ( at least one hour)
For my peace of mind I then put the slides into a 60C oven for 30-60 mins 
before dewaxing.
Please post your opinion/results so far?
Best wishes
Carl



Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge
Kings College London
London
SE1 1UL

020 7848 6813


--

Message: 2
Date: Fri, 18 Oct 2019 23:00:16 +
From: Subash Govender 
To: "histonet@lists.utsouthwestern.edu"

Subject: Re: [Histonet] Histonet Digest, Vol 191, Issue 10
Message-ID:


Content-Type: text/plain; charset="Windows-1252"

Re: How to prevent bubbling in pressure cooker for immunohistochemistry 
procedure.

Maria
Thank you so much for your feedback. The instrument i use for the antigen 
retrieval process is just household 6L Russek Hobbs pressure cooker. Before i 
place my slides into this PC i do actually wait for it to boil and actually do 
see the solution bubble. Then only do i put my slides in and close the lid. I 
am assuming it continues to bubble while reaching its optimum temperature, but 
you think not, so i am unsure. This procedure works quite well as we get good 
results. However, i do get an odd 1 or 2 tissues washing off now and again. So 
to try and stop this completely and to perfect our method i thought about this 
article i read, but sadly have lost. As i do think that maybe no bubbling will 
prevent this. But could be wrong. Will keep searching for the info i lost.
Thanks again, keep well.

Best Regards
Subash

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From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Thursday, October 17, 2019 7:00:01 PM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 191, Issue 10

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Re: [Histonet] Histonet Digest, Vol 191, Issue 11

[Subash Govender via Histonet]

RE:How to prevent bubbling in pressure cooker for immunohistochemistry 
procedure.

Hi there Mark.

Really appreciate your feedback. I  do use tween 20 in the retrieval solution 
but actually thought it was to clean the slides, thus preventing background 
staining. I did not know it prevents bubble formation, so i have learnt 
something. However, i use it at a 0.5 % solution. Maybe thats too much as the 
solution looks a bit soapy, and could also cause lifting of sections im 
presuming.
Thanks again, keep well.

Best regards
Subash

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From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Friday, October 18, 2019 7:00:01 PM
To: histonet@lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 191, Issue 11

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Today's Topics:

1. RELIA HOT JOB ALERT! IHC Specialist for New England area.
Contact me for info ASAP! (Pam Barker)
2. How to prevent bubbling in pressure cooker for
immunohistochemistry procedure (Donovan, Mark)


--

Message: 1
Date: Thu, 17 Oct 2019 14:23:52 -0400
From: "Pam Barker" 
To: "Histopeeps Histonet" 
Subject: [Histonet] RELIA HOT JOB ALERT! IHC Specialist for New
England area. Contact me for info ASAP!
Message-ID: <01ce01d58518$0759e9a0$160dbce0$@earthlink.net>
Content-Type: text/plain; charset="iso-8859-1"

Hi Histonetters!
I hope you are having a great day and cruising into the weekend. I have an
opportunity that I want to share in hopes that you or someone you know might
be interested.
I am looking for a Field Applications Support Specialist ?IHC for a
territory in New England. This is a RELIA EXCLUSIVE!
My client offers an excellent product line and reputation to support it.
This is a full time salaried position NO sales required.
Use your IHC expertise to help my client?s customers further their support
of patient care.
My client is offering a very competitive salary, travel expenses, benefits
and more.
If you would like details on the opportunity please contact me ASAP!

Thanks-Pam

#jobs4myhistopeeps
#ilovemyhistopeeps
#histopeeps
Follow my hashtags and make your day great and your career greater!!

Right Place, Right Time, Right Move with RELIA!

Thank You!
?Pam M. Barker?
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net
https://www.facebook.com/RELIASolutionsforhistologyprofessionals
www.facebook.com/PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia








--

Message: 2
Date: Thu, 17 Oct 2019 23:10:56 +
From: "Donovan, Mark" 
To: "'histonet@lists.utsouthwestern.edu'"

Subject: [Histonet] How to prevent bubbling in pressure cooker for
immunohistochemistry procedure
Message-ID:


Content-Type: text/plain; charset="us-ascii"

Sabash,

What I think you may be referring to is the use of a surfactant in the antigen 
retrieval solution to lower the surface tension. The theory being it reduces 
bubble formation in the retrieval solution at high temperature and hence 
disruption of tissue. Before moving to commercial antigen retrieval solutions 
we would add Tween 20 to a concentration of 0.05% to our in house citrate or 
EDTA retrieval solutions to achieve this effect.

Regards,
Mark


Original message:

Date: Wed, 16 Oct 2019 13:43:28 -0400
From: Maria Cruz mailto:mcruz8...@gmail.com>>
To: 
"histonet@lists.utsouthwestern.edu"
mailto:histonet@lists.utsouthwestern.edu>>
Subject: Re: [Histonet] How to prevent bubbling in pressure cooker for
immunohistochemistry procedure
Message-ID:
mailto:capg7ww1f0dsbw85m8wnrz2pamxpgkqpxw0v9kx-svxmwbv1...@mail.gmail.com>>
Content-Type: text/plain; charset="UTF-8"

Subash:
Since the main purpose of using a pressure cooker for HIER is the rapid and 
reliable pre-treatment of sections while PREVENTING boiling and potential 
tissue detachment, you really shouldn?t be experiencing any bubbling. If you 
are, then your PC isn?t sealed adequately and the only way to fix that problem

Re: [Histonet] Histonet Digest, Vol 191, Issue 10

[Subash Govender via Histonet]

rom: Curt Tague 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] procuring fresh tumor tissue
Message-ID:


Content-Type: text/plain; charset="us-ascii"

Hello again histonet-ers-

I am working with a company do validate a new rapid (another new) tissue 
processor... we are currently in the process of submitting a proof of concept 
paper to CAP then will submit a formal white paper with data. What I am curious 
about is locating tumor tissue that can be used to parallel this study, 
obviously one part processed with the standard process we are all accustomed to 
and the other piece processed with this new technology. We are running all the 
necessary tests to show consistency and comparable results, H&E, special 
stains, IHC, DNA and RNA extraction... what I could use a little help with is 
sourcing some tumor that has not been in formalin for weeks and weeks... 
specifically breast tumor and possibly lung tumor to demonstrate all the 
downstream tests are not compromised with this technology.
Is there any source out there someone could recommend and/or, if you are in a 
large hospital setting, is that something you might be able to assist with, 
when a tumor is identified and not entirely needed for diagnostics?

All the best to you all, thanks for your thoughts,

Curt






--

Message: 5
Date: Thu, 17 Oct 2019 09:21:09 -0700
From: Dave Juliano 
To: Atoska Gentry 
Cc: "histonet@lists.utsouthwestern.edu"

Subject: Re: [Histonet] cleaning old charged slides
Message-ID:

Content-Type: text/plain; charset="UTF-8"

Hi Atsoka! I?d be willing to trade you fresh slides for those foggy ones-
studying effects of age on charges slides. Contact me at: Dave.c.juliano
at gmail dot com if you?re interested!

On Thu, Oct 17, 2019 at 8:24 AM Atoska Gentry via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello,
>
> Has anyone had success cleaning old charged slides which have become foggy
> over time.
>
> Thanks!
> ~Atoska
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--

Message: 6
Date: Thu, 17 Oct 2019 12:27:50 -0400
From: "Pam Barker" 
To: "Histopeeps Histonet" 
Subject: [Histonet] ICYMI here is a link to part 3 of 3 in my series
on Relocation - Relocation an Action Plan. And our latest histology
opportunties on and off the bench!
Message-ID: <014d01d58507$d24bdcb0$76e39610$@earthlink.net>
Content-Type: text/plain; charset="iso-8859-1"

Hi Histonetters,
ICYMI - In case you missed it!!
I am so excited!! My latest article has been published in the NSH Quarterly
Career Center Newsletter under my byline:
Make the Cut
My latest article is entitled: Relocation ? An Action Plan
This is part 3 of 3 in my series on relocation. You can find a link to
parts 1&2 in this article as well!
This is the link to my article on the NSH?s Fixation on Histology Blog and
it is also in the current edition of the NSH Career Newsletter.

Here is the link to the article:
https://www.fixationonhistology.com/post/relocation-part-3-action-plan<https://protect-za.mimecast.com/s/Y4K7CBgX56fEKL03tvyFb_>

If you have a minute to read it I would love to hear what you think.
If you can?t get to the article with this link let me know and I will send
you a copy of it.
Histopeeps, we also have amazing opportunities nationwide!
Leadership and Specialty opportunities:
1. Chicago Greater Chicago area-Field Applications
Specialist ? IHC
2. Boston Greater Boston area-Field Applications
Specialist ? IHC
3. Los Angeles Manager of Customer Training and Support
4. Nashville Histology Supervisor
5. Kalamazoo Applications Specialist
6. Orlando Histology Instructor

HT or HTL or elig opportunities:
1. California Northern CA IHC Specialist
2. California Northern CA Histology tech
3. N. Carolina Raleigh/Durham area Histology Tech
4. Colorado Denver area ? IHC Histology Tech
5. Wisconsin Milwaukee ? Histology Tech
6. Georgia Columbus ? Histotech dermpath
7. S. Carolina Beaufort ? Histology Tech

Please take a look and if you are interested let me know. If you have a
friend who is interested and I place them then I get to give you a referral
reward!

My clients offer competitive pay rates, excellent benefits and in most cases
Relocation assistance or a sign on bonus.
They are ready to interview and hire!!!

If You or Anyone You Know Might Be Interested In a New Opportunity, Please
Contact Me ASAP
call or text me on my cell At 407-353-5070.
If you want some additional information or to set up a time to chat please
call me toll free at 866-607-3542 or email me at rel...@earthlink.net

Have a great day. I look forward to hearing back from you.

Thanks-Pam

#jobs4myhistopeeps
#ilovemyhist

[Histonet] Histonet] Freezing spray in cryostats

[White, Lisa M. via Histonet]

We did use freeze spray in the cryostat years ago.  Then one fateful day we all 
had to go on protocol for exposure to TB because a resident sprayed a lung with 
the freeze spray and there you go.  There is a turn around time for frozen 
sections we are all working with, HOWEVER it is not worth the health and safety 
of Staff to rush the process.  Use the heat extractor and most cryostats have a 
"turbo" cool bar.  Safety first or no one will be left to run the lab.
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