[ccp4bb] P-CUBE workshop on mammalian expression technologies in Oxford

2011-02-22 Thread Peer Mittl
On behalf of the P-cube management team: -Peer Dear All, Don't miss out on the P-CUBE workshop in Oxford in April 2011! Register today under www.p-cube.eu and learn everything about mammalian expression technologies. The registration deadline is March 19, 2011. See you in Oxford! P-CUBE

[ccp4bb] Workshop Diffraction Data Collection Using Synchrotron Radiation

2011-02-22 Thread Manfred S. Weiss
After the successful first two workshops on Diffraction Data Collection Using Synchrotron Radiation, which took place in 2007 and 2009, a third edition of the workshop will be held from July 07-09, 2011 at the Helmholtz Zentrum Berlin fuer

[ccp4bb] linux flavors

2011-02-22 Thread David Roberts
Hello all, Quick question on linux varieties. For years (and years) I have used fedora (after Ultrix of course). In fact, most of my computers are running FC7 (that long ago), it's very stable and works fine. However, since it is no longer supported, I'm toying with upgrading. I upgraded

Re: [ccp4bb] linux flavors

2011-02-22 Thread Roger Rowlett
I switched from FC8 to Ubuntu 9.04 a few years ago. Ubuntu worked with all of my hardware and peripherals out of the box, even newish motherboards, and I had fewer issues with WINE compatibility for CrysalisPro a WIndows-based X-ray data processing program for

Re: [ccp4bb] linux flavors

2011-02-22 Thread David Schuller
Installation of the proprietary nVidia driver is easier with Fedora 14; the hassles with removing the Nouveau driver have been greatly simplified. We use Fedora, although I certainly cannot claim that it has been problem free. It does seem better suited for centrally-managed systems, as

Re: [ccp4bb] linux flavors

2011-02-22 Thread Michel Fodje
The preferred method to get NVIDIA drivers for Fedora is to use the RPM Fusion repositories (http://rpmfusion.org/Howto/nVidia). Drivers installed this way will be automatically updated with the kernel as required. -Original Message- From: CCP4 bulletin board

Re: [ccp4bb] linux flavors

2011-02-22 Thread Xiaoguang Xue
Hi, Maybe CentOS 5 or Scientific Linux 5 is another option for you. Because you have experiences of RPM based distributions, I think you can install and maintain software easily. CentOS and Scientific Linux are based on Red Hat Enterprise Linux 5, so actually they are similarly as Fedora Core.

Re: [ccp4bb] linux flavors

2011-02-22 Thread mjvdwoerd
Dave, We have used CentOS for years and I am very happy with it. We also use NVIDIA hardware. CentOS does not work out of the box with NVIDIA, but NVIDIA has an installation package for their drivers on their web site that does work out of the box in combination with CentOS. That is, you run

Re: [ccp4bb] Could someone can help me to explain why EDTA-2Na can formate salt crystals

2011-02-22 Thread William Scott
What other cations are present? Any divalent cations like Mg++ or Ca++? The Ksp of magnesium phosphate is about 10^-24, so even if you have a very small amount present, say as a contaminant with citrate or EDTA, it will crystallize. On Feb 21, 2011, at 1:22 PM, Yibin Lin wrote: Dear all,

Re: [ccp4bb] Could someone can help me to explain why EDTA-2Na can formate salt crystals

2011-02-22 Thread Yibin Lin
No, there are not any other cations, so I feel very strange. Everything brought from sigma. On Tue, Feb 22, 2011 at 8:58 PM, William Scott wgsc...@ucsc.edu wrote: What other cations are present? Any divalent cations like Mg++ or Ca++? The Ksp of magnesium phosphate is about 10^-24, so even

Re: [ccp4bb] Could someone can help me to explain why EDTA-2Na can formate salt crystals

2011-02-22 Thread Dima Klenchin
No, there are not any other cations, so I feel very strange. Everything brought from sigma. Nothing's strange. EDTA is very poorly soluble at pH 4.2 and would become even less soluble in the presence of 47% PEG. So it crystallizes. Dima On Tue, Feb 22, 2011 at 8:58 PM, William Scott

[ccp4bb] merge and scale high-res and low-res scans with SCALA

2011-02-22 Thread Huiying Li
I have collected two sets of data, high-res and low-res, with the same crystal and integrated them separately with imosflm. Now I want to merge and scale the two MTZ files with Scala in ccp4i GUI. But I do not know how to input 2 MTZ files through the Scala input GUI window (under Data

Re: [ccp4bb] merge and scale high-res and low-res scans with SCALA

2011-02-22 Thread Phil Evans
You can combine them together using the ccp4i task Find or Match Laue group which runs Pointless. This will also try to choose the Laue group and possibly the space group. If the space group is P21 2 21 (not chosen by mosflm which can only choose the Laue group), you can choose in Pointless to

Re: [ccp4bb] Could someone can help me to explain why EDTA-2Na can formate salt crystals

2011-02-22 Thread Liu, Deqian
Your 0.1 M Phosphate/citrate can form crystal at high PEG. Deqian From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Yibin Lin [yyb...@gmail.com] Sent: Tuesday, February 22, 2011 2:24 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Could

Re: [ccp4bb] linux flavors

2011-02-22 Thread Jens Kaiser
David, I'm a big fan of SuSE. the nuveau problem exists, too, but blacklisting fixed it for me. For older hardware I love ultimate linux. The way I understand Zalman stereo it works with everything, given the program you use supports it. I'm sure you are aware of the problem with nVidia

Re: [ccp4bb] linux flavors

2011-02-22 Thread Nian Huang
I vouch for Ubuntu too. FC always has the stability problem for me and I am too tired to find right drivers and compile the programs. I switched after FC8. Ubuntu's repositories seems to be much more reliable and it worked with every program that I am using, including 3D imaging (I am using Nvidia

Re: [ccp4bb] linux flavors

2011-02-22 Thread Ethan Merritt
On Tue, Feb 22, 2011 at 9:16 AM, David Roberts drobe...@depauw.edu wrote: Hello all, Quick question on linux varieties. For years (and years) I have used fedora (after Ultrix of course). In fact, most of my computers are running FC7 (that long ago), it's very stable and works fine.

Re: [ccp4bb] linux flavors

2011-02-22 Thread Nian Huang
I'm downloading ubuntu - is that a good choice? Can I run different flavors of linux with nfs and share drives in a local network (so one has fc7, one has fc13, and another has ubuntu)? Replied too fast and didn't finish your message. I have Ubuntu and most of other machines in NFS are running

Re: [ccp4bb] merge and scale high-res and low-res scans with SCALA

2011-02-22 Thread Laurent Maveyraud
Hi, you first need to be sure that both datasets are indexed the same way (this depends on your spacegroup). During the processing step, you have to give each dataset different batch numbers (directly from imosflm) or you can rebatch them after processing (Data