On behalf of the P-cube management team:
-Peer
Dear All,
Don't miss out on the P-CUBE workshop in Oxford in April 2011! Register
today under www.p-cube.eu and learn everything about mammalian
expression technologies.
The registration deadline is March 19, 2011.
See you in Oxford!
P-CUBE Man
After the successful first two workshops on "Diffraction Data Collection
Using Synchrotron Radiation", which took place in 2007 and 2009, a third
edition of the workshop will be held from July 07-09, 2011 at the Helmholtz
Zentrum Berlin fuer
Hello all,
Quick question on linux varieties. For years (and years) I have used
fedora (after Ultrix of course). In fact, most of my computers are
running FC7 (that long ago), it's very stable and works fine. However,
since it is no longer supported, I'm toying with upgrading.
I upgraded
I switched from FC8 to Ubuntu 9.04 a few years
ago. Ubuntu worked with all of my hardware and peripherals out of
the box, even newish motherboards, and I had fewer issues with
WINE compatibility for CrysalisPro a WIndows-based X-ray data
processing program for ou
Installation of the proprietary nVidia driver is easier with Fedora 14;
the hassles with removing the Nouveau driver have been greatly simplified.
We use Fedora, although I certainly cannot claim that it has been
problem free. It does seem better suited for centrally-managed systems,
as compar
The preferred method to get NVIDIA drivers for Fedora is to use the RPM Fusion
repositories (http://rpmfusion.org/Howto/nVidia).
Drivers installed this way will be automatically updated with the kernel as
required.
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.
Hi,
Maybe CentOS 5 or Scientific Linux 5 is another option for you. Because you
have experiences of RPM based distributions, I think you can install and
maintain software easily. CentOS and Scientific Linux are based on Red Hat
Enterprise Linux 5, so actually they are similarly as Fedora Core. You
Dave,
We have used CentOS for years and I am very happy with it. We also use NVIDIA
hardware. CentOS does not work out of the box with NVIDIA, but NVIDIA has an
installation package for their drivers on their web site that does work out of
the box in combination with CentOS. That is, you run
What other cations are present? Any divalent cations like Mg++ or Ca++?
The Ksp of magnesium phosphate is about 10^-24, so even if you have a very
small amount present, say as a contaminant with citrate or EDTA, it will
crystallize.
On Feb 21, 2011, at 1:22 PM, Yibin Lin wrote:
> Dear all,
No, there are not any other cations, so I feel very strange. Everything
brought from sigma.
On Tue, Feb 22, 2011 at 8:58 PM, William Scott wrote:
> What other cations are present? Any divalent cations like Mg++ or Ca++?
>
> The Ksp of magnesium phosphate is about 10^-24, so even if you have a
No, there are not any other cations, so I feel very strange. Everything
brought from sigma.
Nothing's strange. EDTA is very poorly soluble at pH 4.2 and would
become even less soluble in the presence of 47% PEG. So it crystallizes.
Dima
On Tue, Feb 22, 2011 at 8:58 PM, William Scott
<
I have collected two sets of data, high-res and low-res, with the same
crystal and integrated them separately with imosflm. Now I want to merge
and scale the two MTZ files with Scala in ccp4i GUI. But I do not know how
to input 2 MTZ files through the Scala input GUI window (under Data
Reductio
You can combine them together using the ccp4i task "Find or Match Laue group"
which runs Pointless. This will also try to choose the Laue group and possibly
the space group. If the space group is P21 2 21 (not chosen by mosflm which can
only choose the Laue group), you can choose in Pointless to
Your 0.1 M Phosphate/citrate can form crystal at high PEG. Deqian
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Yibin Lin
[yyb...@gmail.com]
Sent: Tuesday, February 22, 2011 2:24 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Could some
David,
I'm a big fan of SuSE. the nuveau problem exists, too, but
blacklisting fixed it for me. For older hardware I love ultimate linux.
The way I understand Zalman stereo it works with everything, given the
program you use supports it.
I'm sure you are aware of the problem with nVidia and
I vouch for Ubuntu too. FC always has the stability problem for me and
I am too tired to find right drivers and compile the programs. I
switched after FC8. Ubuntu's repositories seems to be much more
reliable and it worked with every program that I am using, including
3D imaging (I am using Nvidia
> On Tue, Feb 22, 2011 at 9:16 AM, David Roberts wrote:
> > Hello all,
> >
> > Quick question on linux varieties. For years (and years) I have used fedora
> > (after Ultrix of course). In fact, most of my computers are running FC7
> > (that long ago), it's very stable and works fine. However, s
"I'm downloading ubuntu - is that a good choice? Can I run different
flavors of linux with nfs and share drives in a local network (so one
has fc7, one has fc13, and another has ubuntu)?"
Replied too fast and didn't finish your message. I have Ubuntu and
most of other machines in NFS are running
Hi,
you first need to be sure that both datasets are indexed the same way
(this depends on your spacegroup). During the processing step, you have
to give each dataset different batch numbers (directly from imosflm) or
you can rebatch them after processing (Data
Reduction/Utilities/Sort-Modify
Hi,
I wanted to use glutathione for cocrystallizing with my protein at about
5mM concentration. What is the concentration of the stock solution to be
prepared for crystallization studies. Do I have to make the stock solution
of glutathione fresh each time or can I make the stock and store at
-20de
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