YSTAL
Sent: 14 April 2008 22:10
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Fwd: [ccp4bb] crystallisation robot
Hi,
Does anyone have information about how long it takes to set up a 96-well
tray for the crystallization robots available? Besides cost per tray
and maintenance cost, ano
ary 18, 2008 2:20 AM
To: <mailto:CCP4BB@JISCMAIL.AC.UK>CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Fwd: [ccp4bb] crystallisation robot
One thing that people often overlook is that quite a lot of protein
can be lost by denaturation on the surface of the drop. This is more
significant for smaller drops.
EMAIL PROTECTED] **
** Headington, Oxford OX3 7BNhttp://www.oppf.ox.ac.uk **
Original message
Date: Mon, 14 Apr 2008 17:10:26 -0400
From: JOE CRYSTAL <[EMAIL PROTECTED]>
Subject: Re: [ccp4bb] Fwd: [ccp4bb] crystallisation robot
To: CCP4BB@JISCMAIL.AC.UK
Lisa A. Nagy, Ph.D.
> University of Alabama-Birmingham
> [EMAIL PROTECTED]
>
> -Original Message-
> From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On
> Behalf Of
> Patrick Shaw Stewart
> Sent: Friday, January 18, 2008 2:20 AM
> To: CCP
Thanks Leo for your introduction.
However, the link is broken.
Please look at the following page.
http://pfweis.kek.jp/protein/Robot/PXS/index-e.html
Best regards,
Yusuke
---
Yusuke Yamada, Ph.D.
Photon Factory
High Energy Accelerator Research Organi
B@JISCMAIL.AC.UK
*Subject:* Re: [ccp4bb] Fwd: [ccp4bb] crystallisation robot
Hi,
Does anyone have information about how long it takes to set up a
96-well tray for the crystallization robots available? Besides cost
per tray and maintenance cost, another important feature we consider
is the time
Dear Joe --
Does anyone have information about how long it takes to set up a 96-
well tray for the crystallization robots available? Besides cost
per tray and maintenance cost, another important feature we
consider is the time for setting up a 96-well tray. It is an
important factor sinc
board [mailto:[EMAIL PROTECTED] On Behalf Of JOE
CRYSTAL
Sent: Monday, April 14, 2008 5:10 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Fwd: [ccp4bb] crystallisation robot
Hi,
Does anyone have information about how long it takes to set up a 96-well
tray for the crystallization robots
CP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Fwd: [ccp4bb] crystallisation robot
Hi,
Does anyone have information about how long it takes to set up a 96-well
tray for the crystallization robots available? Besides cost per tray
and maintenance cost, another important feature we consider is the time
for se
P4BB@JISCMAIL.AC.UK <mailto:CCP4BB@JISCMAIL.AC.UK>
Subject: [ccp4bb] Fwd: [ccp4bb] crystallisation robot
One thing that people often overlook is that quite a lot of protein
can be lost by denaturation on the surface of the drop. This is more
significant for smaller d
gt; --
> > > Lisa A. Nagy, Ph.D.
> > > University of Alabama-Birmingham
> > > [EMAIL PROTECTED]
> > >
> > > -Original Message-
> > > From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
> > > Patrick Shaw Stewart
>
ilto:[EMAIL PROTECTED] On Behalf Of
Patrick Shaw Stewart
Sent: Friday, January 18, 2008 2:20 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Fwd: [ccp4bb] crystallisation robot
One thing that people often overlook is that quite a lot of protein
can be lost by denaturation on the surface of the drop. T
aw Stewart
> Sent: Friday, January 18, 2008 2:20 AM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: [ccp4bb] Fwd: [ccp4bb] crystallisation robot
>
> One thing that people often overlook is that quite a lot of protein
> can be lost by denaturation on the surface of the drop. This is more
> s
ECTED]
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
Patrick Shaw Stewart
Sent: Friday, January 18, 2008 2:20 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Fwd: [ccp4bb] crystallisation robot
One thing that people often overlook is that quite a lot of protei
___
>
>
> From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
> [EMAIL PROTECTED]
> Sent: Wednesday, January 16, 2008 8:31 PM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] crystallisation robot
>
>
>
>
>
> Once upon a time I worked in a
ifferent, the end point
is not, and this can significantly affect the outcome of the experiment.
Mark
-Original Message-
From: Oganesyan, Vaheh <[EMAIL PROTECTED]>
To: CCP4BB@JISCMAIL.AC.UK
Sent: Thu, 17 Jan 2008 7:40 am
Subject: Re: [ccp4bb] crystallisation robot
heh
Sent: Thursday, January 17, 2008 8:41 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] crystallisation robot
Mark,
What was the state of the larger drops when tiny counterparts had
crystals? My guess - they all precipitated.
I'm trying to understand why some proteins or some condi
January 16, 2008 8:31 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] crystallisation robot
Once upon a time I worked in a group that was interested in developing
crystallization in microfluidics. This was before the time that Fluidigm
existed and we had not heard of crystallization with
s not a flame, even if heat might be a good thing on a night with
> temperatures predicted far below 0F.
>
> ?:-)
>
> Mark
>
>
>
>
>
>
>
> -Original Message-
> From: Anastassis Perrakis <[EMAIL PROTECTED]>
> To: CCP4BB@JISCMAIL.AC.UK
> Sent: W
CCP4BB@JISCMAIL.AC.UK
Sent: Wed, 16 Jan 2008 6:17 am
Subject: Re: [ccp4bb] crystallisation robot
> Oryxnano 50+50 nL?
>?
> Demetres?
>?
?
Which, indirectly, brings up an interesting (but not relevant to the Oryx)
question.?
?
Nucleation is a process that does have a stocha
CCP4BB@JISCMAIL.AC.UK
Sent: Wed, 16 Jan 2008 6:17 am
Subject: Re: [ccp4bb] crystallisation robot
> Oryxnano 50+50 nL?
>?
> Demetres?
>?
?
Which, indirectly, brings up an interesting (but not relevant to the Oryx)
question.?
?
Nucleation is a process that does have a stocha
Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
Oganesyan, Vaheh
Sent: Wednesday, January 16, 2008 9:06 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] crystallisation robot
...
Once in a while I get large crystal that can be used for data
collection, but in most of
Vaheh,
If I'm optimising in 24 well trays after a hit in 96 I generally use
sitting drop trays (cryschem) as a first step and try different ratios
of protein:mother liquor first rather than changing the protein
concentration. I know that sometimes the crystals stick to the plastic
but I use th
Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
Anastassis Perrakis
Sent: Wednesday, January 16, 2008 8:23 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] crystallisation robot
> More recently, I've looked at all of the crystallization robot
Regarding stochastic processes: to increase the chances of nucleation
one would like to have protein in the right nucleation zone for a
certain period of time.
If the drop becomes too small, the ratio evaporation surface/drop
volume changes. So, the nucleation zone might be reached within the
More recently, I've looked at all of the crystallization robot
vendors.
For single lab users, all of the systems work well. Systems like
the Hydra
or Mosquito are less automatic, but provide the basic functions for
crystallization trial setup. For more of a user facility with a large
number of
Oryxnano 50+50 nL
Demetres
Which, indirectly, brings up an interesting (but not relevant to the
Oryx) question.
Nucleation is a process that does have a stochastic aspect.
Thus, one could argue that compromising to 200-300 nl might be better
than either extremes of 50nl (too small volum
In our original work on a prototype, we used the Cartesian technology. We
were able to dispense 10nL+10nL drops with a large range of viscosities
without difficulty. The main issue was to wash the tips after use to
prevent clogging. That was with a system that could dispense 1nL droplets.
The prese
Oryxnano 50+50 nL
Demetres
David Briggs wrote:
I'll defend the honour of the phoenix... (again)
Bernhard Rupp 100+100 nl
Dave Briggs (and all users at Univ of Manchester, UK) 100+100nl
Others..
Only time we have ANY problems is when the nano dispensing tip gets
clogged. Often a good wa
I'll defend the honour of the phoenix... (again)
Bernhard Rupp 100+100 nl
Dave Briggs (and all users at Univ of Manchester, UK) 100+100nl
Others..
Only time we have ANY problems is when the nano dispensing tip gets clogged.
Often a good wash whilst still on the machine will clear the blockage.
D
: Wednesday, January 16, 2008 1:53 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] crystallisation robot
Dear Alexey,
being involved in the development of the 'fixed needles + a few'
robots and 96-well plates early on, I wonder about your bad experiences.
You seem to say that t
y, January 14, 2008 9:36 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] crystallisation robot
we have recently tried the new lid mentioned below. It works very well,
Mosquito makes 3 hanging drops 96 well plate in 3-4 minutes! The film is very
easy to handle and it is very transparent. The pri
-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Shirley Roberts
Sent: Friday, January 11, 2008 7:25 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] crystallisation robot
I sent this personal reply on wednesday to Madhavi Nalam's original question.
In the light of r
Dear all -
Under the BIOXHIT home page,
http://www.bioxhit.org
you can navigate to "Section 1" or "HTP Crystallization",
or simply follow the link below:
http://icarus.embl-hamburg.de/bioxhit/bioXHITSection1.jsp
It has quite a lot of data on what people use, available facilities,
and testin
Thanks to all who replied to my query.
Madhavi
I sent this personal reply on wednesday to Madhavi Nalam's original
question.
In the light of recent comments I'm posting it to the BB to support TTP
labtech and Mosquito!
Some of the points have been made by others now, excuse the repeats!
We at YSBL have been very happy with our mosquito. We
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