Jim,
I must say that I have used Eukitt exclusively for over 13 years and
the only problem I have had with Eukitt is when I do not use enough
and especially when coverslipping thicker sections. It is xylenes
based so maybe you are experiencing excessive evaporation during
drying??? If
Louise has very good advice here as related to paraffin processing of
this tissue. I may even add to soak the block a little more before
taking the final sections. However, have you ever thought of
processing into MMA resin? If you have these capabilities you may be
very pleased with the
Dear Cornelia Jack
i disagree about the soaking as this will negate the chilling effect of the
freezer. The whole idea is to have the block as cold as possible to get
maximum support from the wax*. If the tissue has been processed and
fixed properly, there should not be a necessity for
Thanks Gayle for your input.
Trouble is, i like the TDE system, and despite not knowing what's in the
solution the IHC results are great. I wasn't sure if endpoint testing could
be used with other acids besides HCl, but you have answered my doubts on
that score. I suspect that, from the smell,
Hi Reul, It is possible that you are not processing your bone long enough in
the tissue processor. If you would like to discuss this possibility, please
email me. Helen
Helen F Wimer HT (ASCP)
Smithsonian Institution
Department of Vertebrate Zoology
Washington, DC
(301) 496-1391
Hi Sarah,
I used to make a ton of cytospins. I would air-dry them, fix them in whatever
(ethanol or acetone/ethanol), air-dry them again and then wrap the slides in
foil, box them up and store @ -80C. The slides lasted for months!
When taking them out to use for staining, be sure to allow
Thanks to all who replied to my post about bunsen burner / forcep
sterilization alternatives. Many of you even included links that were
really helpful. All of your input was very much appreciated!
Brandi Higgins, BS, HT(ASCP)
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That is my understanding as well.
Cheryl A. Miller HT(ASAP)cm
Histology/Cytology Prep Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4145 ext. 554
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf
Hi all,
I am looking for Gillette super stainless Inoxydable Blades (10031694)
for our vibratome. Apparently Gillette does not make this blade
anymore. I bought some feather blades from Ted Pella and they tore up
my tissue badly (40 micron sections).
Alternatively would anybody know of a good
The regs say at least 60 semesters hours (or equivalent) from an accredited
institution are required, and at least 24 of those hours need to be in specific
sciences. See below for specifics.
Brent Jeter
Anatomic Pathology Supervisor
The George Washington University Hospital
202-715-5076
Can you charge for two different stains for the urine cytospin?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histonet-requ...@lists.utsouthwestern.edu
Sent: Tuesday, May 18, 2010 12:43 PM
To:
Hi Hadley,
We get our blades from EMS (Electron Microscopy Sciences):
http://www.emsdiasum.com/microscopy/products/preparation/blades.aspx?mm=10#71990
On this page you'll find the injector blades. Further down they have the double
edge blade you can break in half and use in the holder. Try
What do others do when validating a new model of a piece of equipment -
same manufacturer, same basic staining process, but an updated version
of the equipment?
I've been told the protocols should be the same and that we only need to
run three controls with three different but similar
Good Morning to All,
A few years ago I sent a inquiry to Histonet asking if anyone has ever had this
issue in their tissue processing area? We are having this issue again and I
cannot pinpoint any changes or issues that have arisen.
We have our VIP tissue processor, paraffin pot and embedding
Has anyone run into a problem or artifact from freezing spray? I think we may
be having a problem with it but I can't find any pictures or descriptions of
what it looks like.
Thanks in advance,
Erin
Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248
The thickness of tissue would work better if thinner, however, if you can
infiltrate in paraffin overnight for an extra 12-18 hours it should embed and
section just fine. I face the block warm; soak on ice for a short time and
then section. I find that taking the cuts in deeper, after the
You would think the ASCP would have some kind of grossing tech qualification
or certification for those who aren't PA's but have the 60 semester hours
and training. Just thought I'd throw that out there.
Mark Tarango
On Wed, May 19, 2010 at 7:36 AM, Jeter, Brent
Check with ViroStat (207-856-6620) in Portland, ME. They have some excellent
infectious disease antibodies.
Richard
Richard W. Cartun, Ph.D.
Director, Histology Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour
There is a text written by the late Lee Luna and Samuel Wesley Thompson
entitled An Atlas of Artifacts in this the artifact from freeze spray is
pictured. The ISBN # is 0-398-03624-1 Published by Charles C. Thomas,
Publisher.
Shirley Powell
-Original Message-
From:
We perform our entire validation process as a new piece of equipment.
Our validation protocols are quite extensive, up to about 85 pages long
on each piece of major equipment, at least that's what it was for our
new prisma stainer and glass coverslipper. We perform an
installation/operational
Erin,
Holding the can too close and/or spraying for a prolonged period both cause
freeze artifact. Look at your block, it will not have a smooth look to the
paraffin. it will have lines it much like a cracked piece of glass. the tissue
will appear cracked on the waterbath and will even separate
Erin,
One can certainly get cracking of the tissue and surrounding paraffin
from a too intense application of freezing spray. This is usually
grossly visible, at least in the paraffin.
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Hello All,
We have new microwave tissue processor, until this purchase all tissue
has been placed on a standard VIP processor. My medical director would
like for most of our 125 antibodies worked up on microwave processed
tissue. I have been challenged with getting specimens to process on the
Can you charge for two different stains for the urine cytospin?
The answer to this question is a depends. If you are just doing any
of the optional stains that may be used on a cytology preparation
(namely Pap, HE, romanovsky) you are NOT permitted to charge for each
of these (they are not
Just to clarify or perhaps cloud the picture a little more
Not all grossing is grossing.
So when we're talking about transferring small biopsies, in their
entirety, from a formalin container to a cassette, and describing the size,
number, and color of the tissue pieces submitted, with no
We are in need of a temporary, ASCP certified histotech for 4-6 weeks beginning
June 21 at El Centro Regional Medical Center. Located just 2 hours east of San
Diego, 100 miles south of Palm Springs and only 60 miles west of Yuma. Our new
lab was just completed in February, 2010 and is
Hi Laurie,
I have a Benchmark Ultra and a Benchmark XT from Ventana and they follow the
basic steps, similar protocols and I needed to revalidate everything. They are
sufficiently different (in my experience) that it warranted a complete
revalidation. A part of the reasoning for this was
Does any one use or require gloves when cutting and embedding? How about
safety goggles when embedding?
Thanks in advance-
Cindy DeRiso
Yale University Pathology
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[DEL: 3D :DEL] = 3D
Picture on the left has the artifact, picture on the right is good
to go. Hope this= helps!
Sarah Goebel, B.A., HT (ASCP)
Histotechnician
= /div
XBiotech USA Inc.
8201 Eas= t Riverside Dr. Bldg 4 Suite 100
= Aus= tin, Texas 78744
Things have changed. All grossing is back to being grossing.
Jan Mahoney
Omaha, NE
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniel Schneider
Sent: Wednesday, May 19, 2010 12:58 PM
To:
I require gloves when sectioning only because it helps eliminate
epithelial floaters, with respects to embedding the tech can choose to
or not to use gloves. No safety goggles are required.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder,
Really. Are you sure?
Received in formalin, labeled with the patient's name and number, the
specimen consists of a single tan-gray fragment of tissue measuring 3
millimeters in greatest dimension, submitted entirely in A1 requires 60
some odd hours?
I have no gripe with requiring a certain
Can anyone tell me if there is a specific question on the CAP checklist
that addresses revalidation of antibodies when starting up a new IHC
stainer?
Laurie Colbert
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Hi Brandi,
The electric three well forceps warmer that Jackie mentioned is really handy
and you can find it at most medical supply companies. They sell the exact same
unit but for widely different prices. It's in the Cardinal catalogue for $600
Cat. No. M7323, Mfr. No. FW-120. Mercedes Medical
I will be out of the office starting 05/19/2010 and will not return until
05/25/2010.
Please contact Kelly Miner at 617-871-5122 if you have any questions
regarding clinical trial samples.
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Laurie
I'm not aware of a particular question, but I would believe you would
have to perform some validation steps for each antibody. I would
approach it the same way you approach validating new lots of antisera.
The CAP paper on standardization of IHC recommends 25 different samples
when you
Erin -
Try this trick when using the freezing spray: take a folded kim wipe
tissue and fold it again, then again then the long fold is folded twice so
you end up with roughly a 1 inch by 1 inch multi layer slide wiper. Run
your finger down each crease and after the final fold, give it a
One thing i forgot to mention wasthat when you embed, try
to orientate the
tissue so that the long axis (if there is one) lies in the
same direction as
the cutting stroke. when embedding, orientate the tissue at
a slight
diagonal, so that the knife dous not continously pass
through the tissue on
Does anyone have Peggy Wenk email or phone.
Thanks
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I have seen a lot of traffic on the site regarding bone
processing...If anyone is interested, I have personally
witnessed the following system being used very successfully
for the embedding and sectioning of bone, including bone
that has been surgically implanted with metal devices.
I just
Thanks to all who've been most helpful.
I probably shouldn't be admitting it, but I don't think I had enough
college science courses to be allowed to gross today. Maybe if they
don't find out that two of my biology courses were in paleontology. I
can gross a trilobite like you wouldn't believe!
We also had the appearance of burnt tissue when someone was using the
spray excessively.
Sebree Linda A lseb...@uwhealth.org
Sent by: histonet-boun...@lists.utsouthwestern.edu
05/19/2010 11:07 AM
To
Martin, Erin erin.mar...@ucsf.edu, histonet
histonet@lists.utsouthwestern.edu
cc
Subject
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