never knew what time it was
but we knew how sublime it was.
E. Wayne Johnson DVM
Enable Ag Tech
Beijing.
Greg Dobbin via Histonet wrote:
This is more of a survey than a question:
For those of you tracking and documenting your cold ischemic times for
breast tissue (ie time out of body to time
//youtu.be/UDfAdHBtK_Q?t=445
E. Wayne Johnson DVM
Enable AgTech
Beijing
Terri Braud via Histonet wrote:
Sorry, E. Wayne, but in the USA, according to December 2004 JAMA The Journal of
the American Medical Association 292(20):2500-5, recent examination of these
issues by a US federal cour
l property and the mining of it from your body gives the hospital no
particular right to take control of it
any more than they have the right to take control of a birthed infant.
E. Wayne Johnson DVM
Enable AgTech
Beijing
John Garratt via Histonet wrote:
I suggest that path labs start a discussion
I wanted my femoral heads after a dual hip replacement.
I was refused as it was against local policy but the surgeon
was kind enough to take some pictures for me and sawed through one sagitally
so that i could see the pathology.
E. Wayne Johnson DVM
Enable AgTech
Beijing
Cartun, Richard via
stodgy and unfriendly and hard to
get along with.
E. Wayne Johnson, DVM
Enable AgTech
Beijing
Terri Braud via Histonet wrote:
We were using mModal and are in transition to Voicebrook which uses Dragon as a
speech recognition set up with SQ CoPath for both grossing and microscopic
dictations. We
You could warm the paraffin in an oven overnight
to melt it so that the heat of fusion is not extracted
from the embedding center paraffin tanks when it is added
as melted paraffin at 58-65 degrees.
Pairan, Kelly via Histonet wrote:
Good Afternoon,
Recently we starting taking the daily maximum
In China we can buy Leica, Feather, or domestic.
The domestic ones are not uniformly good or bad.
I am really really tired of Feather and I consider that my time
is worth something so I insist that we use Leica although they
are as "high as a cat's back".
> ---Original Message---
>
modes on the machine could be the difference
between joy
and the slough of despond.
E. Wayne Johnson DVM
Enable AgTech
Beijing
Patpxs via Histonet wrote:
Hi Garrey,
The answer is “it depends”. What you do when a processor fails depends on the
failure point. If the tissue is still in dehydrant
rush
to get the results
so the CDC's C.Y.A. time doesn't cause trouble but waiting 72 hours for a
surgical path result
would seem to be just wasting time.
E. Wayne Johnson DVM
Enable AgTech
Beijing
Tony Henwood (SCHN) via Histonet wrote:
Hi Richard,
It will depend on the size of the tissue
der to overcome "friction"."
E. Wayne Johnson DVM
Enable AgTech
Beijing
Mark Tarango via Histonet wrote:
I had heard that CLIA was relaxing things and is not requiring a new # to
work from home right now. Best to check on the regulatory but FFPE isn't
typically infectious. The
And I appreciate Bryan Llewellyn and the other old experienced hands
and I really like Gray and Humason and even Lillie, and the others and the
way they wrote and the way they thought,
and their delight and fascination with the world they were discovering.
E. Wayne Johnson
Enable AgTech
Beijing
(as a substitute for incineration).
E. Wayne Johnson DVM
Enable AgTech
Beijing
Paula via Histonet wrote:
Hello,
Has anyone ventured out and looked into a UV light instrument, like a box
that can kill any bloodborne pathogens that are on paper (grossing
area..where the patient req form is used
I am skeptical that parts are not available for these relatively new
(fifteen years old) machines...
Of course reliability is a serious concern in any case.
E. Wayne Johnson DVM
Enable AgTech
Beijing
Etheridge, Sandra AGRI:EX via Histonet wrote:
Hi everyone,
We are looking to purchase a new
The vet samples can be processed just any other animal tissue whether they be
human or rat. People is animals too. Formalin fixed there are no special safety
issues. E. Wayne Johnson DVM Enable AgTech Beijing ewj Email:e...@pigs.ag
Signature is customized by Netease Mail Master On 04/16/2019 08
and process routinely to
paraffin
and stain with our H & E.
-
E. Wayne Johnson
Enable AgTech, Beijing.
"Never do anything mean to a pig. They'll squeal on you every time."
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(207) 373-6573
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E. Wayne Johnson 朱稳森博士
Enable AgTech Consulting
恩睿康农业技术咨询有限公司
Beijing
188 1088 3205
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We have our own lab in Beijing and we do both of those things.
We have a consulting veterinary practice and the main purpose of the lab
is to support that veterinary practice. It would be unusual in the USA
to have histopath in a vet lab but in China there was no diagnostic
laboratory that
Xylene is becoming more and more of a nuisance material and a problem
for us in use and in disposal. We are still able to use it but with
increasing concern.
We have been able to eliminate xylene from our staining procedures
altogether in our small laboratory.
We use a hair dryer to dry
loved
hands like any staining powder. Store it wet, but the /~~paranoia~/~
about picric acid is exactly that.
And on the part of the cops and bomb squads who take picric acid out and
detonate it amid much drama and fanfare, heavy on the delusions and
illusions of grandeur.
E. Wayne Johnson
Bromcresol means Bromocresol.
Faint not, but I certainly can't say you won't dye if you leave out the O.
The o is subject to elision due to its difficulty in pronounciation.
Such an occurrence of elision between 2 consonants is called syncope.
In English writing an elided vowel is often
Tattooing is not only for dissidents, miscreants, the wayward,
and the Llewellyn-ites among us.
Sheep, rabbits, pigs, cattle, horses, stoats, and goats can be tattooed
also,
not so much of an expression of individuality as the need for permanent
identification.
Face velocity is simply the airflow rate in CFM divided by the area of
the hood opening in square feet.
A smaller opening at the same flow rate gives a higher face velocity.
Titanium tetrachloride in a small plastic squeeze bottle can be used to
generate smoke.
On 3:59 AM, WILLIAM DESALVO
We don't do in-situ PCR, but the principle is that with formalin-fixed
tissues
your amplified product is trapped in the protein matrix on the slide.
On 3:59, Sarah Dysart wrote:
Anyone out there do this? If so, during the PCR step you are amplifying your
gene of interest, where does the
We work in the veterinary medical field and 98% of our work is with
swine diseases
including some feed additive safety evaluations. We provide pictures of
all the lesions
that we see as part of the report we provide to farms. It definitely
takes more of our
time than it would to give a
While you're at it, maybe some one can explain what the
-wah- in diddy-wah-diddy means?
On 3:59, Morken, Timothy wrote:
Just like Formalin, a brand name now used interchangeably with formaldehyde.
And your trivia of the day:
Formaldehyde was the first polyatomic organic molecule detected in
At one time I did a lot of work on cartilage and growth plate
and used Toluidine Blue and Fast Green. T-blue (buffered appropriately)
stains the
proteoglycan a lovely metachromatic blue and the bone and most
everything else
is green. The nuclei of cells are also green since we used no nuclear
enzyme mix for this digestion?
Yes I am considering in-situ hybridization but we have working PCR methods
so that seems to be a simple logical step.
E. Wayne Johnson
Enable Ag Tech
Beijing.
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A stingy person is called an Iron Rooster (tie gong ji) in Chinese.
An iron rooster won't turn loose of even one feather.
The scratches that appear on slides cut with blades that should have
been changed --we call
them iron rooster tracks. Sort of a pun since many people think Chinese
Jack,
That sounds really awesome.
I did some work with the teeth of sows (female pigs) from
specimens collected at slaughter. Those are very difficult to decalcify,
and when finished, are likely to have no nuclear detail remaining.
Interested to hear what you learn
Wayne Johnson
Beijing
I intend to make us some of these tools.
It's a great idea.
On 3:59, Rene J Buesa wrote:
How much squeezing? Tissues have certain elasticity and after the squeezing they will bounce
back as a thicker slice. Free hand sectioning I think is always better.
René J.
From: Weems, Joyce
I read one paper or abstract recently where sections were made at 50
microns then used
successfully for RT-PCR. I am pretty sure that it was PRRS virus that
they were pursuing.
On 3:59, Mark Tarango wrote:
I would try getting more sections from LCM and extracting into 7.5 uL, if
that
Rene' ,
What procedure do you recommend for Giemsa staining of tissues?
There seem to be several different variant protocols out there.
Wayne
Enable (Enruikang) AgTech Consulting
Beijing
On 10/14/12 22:56, Rene J Buesa wrote:
Try Giemsa for tissues
René J.
Pam Plumlee thinks you're cute.
On 10/14/12 2:41, Akemi Allison wrote:
Take me off this list! We are not a dating service!
Akemi Allison BS, HT(ASCP)HTL
Director
Phoenix Lab Consulting
E-Mail: akemiat3...@yahoo.com
From: Pam
If you do that and announce your availability on Histonet, you are likely
to be criticized publicly by a few, while others send email and twitters
to one
another privately discussing your attributes, and those who like having
the information
that you are available probably will not speak up in
I got it open and cleaned the wheel stop mechanism and
the belt in front and it works good now and we now have a backup and
my people are smiling.
Thanks for everyone's helpful advices.
Wayne Johnson
Enruikang (Enable) Ag Tech
Beijing
On 9/22/2012 4:34 AM, Burton, Lynn wrote:
Perhaps they
We work in a previously underutilized histology lab.
There was a sliding horizontal Sakura microtome, a functioning Thermo
microtome,
and a nonfunctioning Leica 5xxx (not a very fancy Leica).
The center where we work has no interest in fixing the Leica microtome.
I am supposing that it is
I think its a Leica 2125.
On 9/22/2012 12:32 AM, E. Wayne Johnson wrote:
We work in a previously underutilized histology lab.
There was a sliding horizontal Sakura microtome, a functioning Thermo
microtome,
and a nonfunctioning Leica 5xxx (not a very fancy Leica).
The center where we work
Tech One seems to be limited in scope to the US.
Nice hearing from you in Galesburg. I practiced pig production medicine
in Illinois for quite a few years.
Doug Hoefling the former director and pathologist in Galesburg
is an old friend and mentor of mine, but of course he is long since
What danger of Picric Acid are you concerned with?
Surely its not the hyped explosion hazards.
We use picric acid and as inquisitive boys we have tried very hard to
ignite it thinking it would be fun.
We dried some down and wrapped it in aluminum foil and with appropriate
protection
and pop-in the slides
in the oven.
Balsam of Canada (the resin) is dissolved in xylene (always) so the
penetration is also assured.
Under separate cover I am sending you my articles.
Try this method, you will love it!
René J.
*From:* E. Wayne Johnson e...@pigsqq.org
*To:* Rene J Buesa rjbu
in their papers. Ok.
*
E. Wayne Johnson
Enruikang Ag Tech
Beijing
On 9/13/2012 8:41 AM, Tony Henwood (SCHN) wrote:
Yep
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
I am convinced to give it a try because I also have trouble will the
loss of some stains in dehydration.
I was concerned that the slides would not clear well after oven
dehydration. I will see how it
works for me.
I can see clearly how going from counterstain to oven will save much
hassle
.
The aqueous HCl seems to be working and is not harming the nuclei so I
may have a sort of solution
and am calling it calcium crystals in the water until I know better.
I may look for some sort of filter to put in the water line.
E Wayne Johnson DVM
Enruikang Ag Tech
MOA Feed Industry Centre
Thanks all for many useful and helpful suggestions
and interesting anecdotes.
E. Wayne Johnson, DVM
Enruikang AgTech
MOA Feed Industry Centre
China Agricultural University
Beijing
On 8/22/2012 5:27 AM, David A. Wright wrote:
Hi Wayne Histonet
My guess is that Wayne's crystals
microscopically at every step and
try to find at which step the problem is occurring.
Any thoughts or similar experiences?
E. Wayne Johnson, DVM
Enruikang AgTech
MOA Feed Industry Centre
Beijing
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