Re: [Histonet] Oil Red O

l for the student, practicing technologist, and resident-in-pathology www.amazon.com John Kiernan London, Canada = = = From: Niihori, Maki - (mniihori) via Histonet Sent: March 5, 2021 4:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Oil Red O We wo

Re: [Histonet] Oil Red O

-pathology > www.amazon.com > John Kiernan > London, Canada > = = = > > From: Niihori, Maki - (mniihori) via Histonet > > Sent: March 5, 2021 4:08 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Oil Red O > > We would like to stain Right V

Re: [Histonet] Oil Red O

gy: A manual for the student, practicing technologist, and resident-in-pathology www.amazon.com John Kiernan London, Canada = = = From: Niihori, Maki - (mniihori) via Histonet Sent: March 5, 2021 4:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histon

Re: [Histonet] Oil Red O

What have you fixed the tissue with? Can you cut frozen sections from the fixed tissue? This is useful information to start with. John On Fri, Mar 5, 2021 at 1:08 PM, Niihori, Maki - \(mniihori\) via Histonet wrote: > We would like to stain Right Ventricle (RV) and Lung (both from rat samples

[Histonet] Oil Red O

We would like to stain Right Ventricle (RV) and Lung (both from rat samples) with Oil Red O. I appreciate if anybody can share a good protocol/kit information with me. Thank you, Maki * Maki Niihori, PhD Life Sciences North Rm# 402, Department of Medicine, The Univer

Re: [Histonet] Oil Red O permanent mountant

UNCLASSIFIED Hi again, Meant to say ThermoFisher Aqua Mount, not Permount in my previous post. Apologies, Chris Hagon | Senior Scientist, Anatomical Pathology ACT Pathology | health.act.gov.au Phone (02) 5124 2874 --

[Histonet] Oil Red O

to make up. I usually only see the precipitate with old solutions. Just make what you use within a few weeks. Best 'o luck, Amos > > Message: 2 > Date: Wed, 3 Oct 2018 15:26:25 + > From: Reuel Cornelia > To: "histonet@lists.utsouthwestern.edu" >

Re: [Histonet] Oil Red O

Hi Reuel, In my experience this is the ORO crystallizing out on the section. I would first filter your staining solution, then keep your staining times to as short as you can. Also changing between 37 and room temperatures may be useful too. mills ᐧ On Wed, Oct 3, 2018 at 8:39 AM Reuel Cornelia

[Histonet] Oil Red O

I was wondering if you could help me know why our Oil Red O have some black snowflakes on our fat tissue hours after staining or after 24 hours more snowlflakes precipitation occurs. The staining was reference was from Lillie RD, Ashburn. Please note that our staining works well between two to

[Histonet] Oil Red O

Hello all, Will Oil Red O stain phospholipids? We will be helping another lab with some work and the project out line states that Phopslipids are externalized on the cell surface during apoptosis. Can always try it I suppose. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Co

[Histonet] Oil Red O

Hello Fellow Histonetters, We often get slides from another hospital for Oil Red O staining. On occasion, they send them at room temp. and until they get to us it's been a day or more. I'm worried that because they are unfixed the cells will be autolyzed and I don't want to charge the patient

[Histonet] Oil Red O staining question

Experts! Is there any reason you would not consider formalin fixation followed by cryoprotection/cryosectioning for Oil Red O (or bodipy for that matter) staining of mouse muscle? I ask as many folks seem to have difficulty in the flash freezing aspect of tissue collection and end up with lot

[Histonet] oil red o on cytology

What kind of control do you use when looking for fat in a BAL specimen? This stain is done on a cytospin slide. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.36

RE: [Histonet] Oil red O

: Jennifer MacDonald CC: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu; P.E. Visser Onderwerp: Re: [Histonet] Oil red O It is for Sudan Red 5B. All these "apothecary" names are essentially meaningless René J. From: Jennifer MacDonald < <mailto:jmac

Re: RE: [Histonet] Oil red O

, September 09, 2013 2:00 PM To: Rene J Buesa; Ada Feldman; histonet Subject: RE: [Histonet] Oil red O I believe the answer should be - Oil Red O is a brand name (not uncommon in the dye indutry) and represents the two dyes found in the compound, Solvent Red 27 and Sudan Red 5B William DeSalvo, B

Re: [Histonet] Oil red O

: Monday, September 9, 2013 3:40 PM Subject: [Histonet] Oil red O Hi all I was requested where the O stands for. who has any suggestion. Regards Piet Visser Histotech Bronovo The Netherlands ___ Histonet mailing list Histonet

Re: [Histonet] Oil red O

I believe the question is what is the "O" signify. as in OG6 the O is for orange. From: Rene J Buesa To: "P.E. Visser" , "histonet@lists.utsouthwestern.edu" Date: 09/09/2013 12:46 PM Subject: Re: [Histonet] Oil red O Sent by:histonet-bo

RE: [Histonet] Oil red O

uthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, September 09, 2013 2:45 PM To: P.E. Visser; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Oil red O Do you mean that you were asked to do Oil Red O (ORO) stain? It is described in any tech

Re: [Histonet] Oil red O

"Goins, Tresa" To: "Grantham, Andrea L - (algranth)" Cc: HISTONET Sent: Monday, September 9, 2013 4:11 PM Subject: RE: [Histonet] Oil red O May be the position of the active group on the benzene ring - "ortho" vs. "meta" vs. "para&quo

RE: [Histonet] Oil red O

al Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Monday, September 09, 2013 2:00 PM To: Rene J Buesa; Ada Feldman; histonet Subject: RE: [Histonet] Oil red O I believe the answer should be -

RE: [Histonet] Oil red O

: adafeld...@anatechltdusa.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Oil red O > CC: > > There you have it! > René J. > > > > From: Ada Feldman > To: histonet@lists.utsouthwestern.edu > Sent: Monday, September 9,

Re: [Histonet] Oil red O

There you have it! René J. From: Ada Feldman To: histonet@lists.utsouthwestern.edu Sent: Monday, September 9, 2013 4:13 PM Subject: Re: [Histonet] Oil red O As you are finding out the answers to dye nomenclature you can had these endings to your list of the

Re: [Histonet] Oil red O

mber 9, 2013 3:53 PM Subject: Re: [Histonet] Oil red O I believe the question is what is the "O" signify. as in OG6 the O is for orange. From:  Rene J Buesa To:    "P.E. Visser" , "histonet@lists.utsouthwestern.edu" Date:  09/09/2013 12:46 PM Subject:        Re:

RE: [Histonet] Oil red O

501 F- 215-993-6803 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of P.E. Visser Sent: Monday, September 09, 2013 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Oil red O Hi all I was requested whe

Re: [Histonet] Oil red O

3:49 PM Subject: RE: [Histonet] Oil red O I believe the question is:  what does the "O" in Oil Red O stand for? Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI  53792-5132 -Origina

[Histonet] Oil red O

Hi all I was requested where the O stands for. who has any suggestion. Regards Piet Visser Histotech Bronovo The Netherlands ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histone

Re: [Histonet] Oil red O

_ From: "Grantham, Andrea L - (algranth)" To: Cc: HISTONET Sent: Monday, September 9, 2013 4:06 PM Subject: Re: [Histonet] Oil red O Rene, You still have not answered the question - what does the "O" stand for? Andrea Grantham, HT (ASCP) Senior Research Specialist

Re: [Histonet] Oil red O

n.edu] On Behalf Of P.E. Visser > Sent: Monday, September 09, 2013 3:41 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Oil red O > > Hi all > > I was requested where the O stands for. who has any suggestion. > > > > Regards Piet Visser >

RE: [Histonet] Oil red O

th) Sent: Monday, September 09, 2013 2:06 PM Cc: HISTONET Subject: Re: [Histonet] Oil red O Rene, You still have not answered the question - what does the "O" stand for? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology

Re: [Histonet] Oil red O

Rene, You still have not answered the question - what does the "O" stand for? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu

RE: [Histonet] Oil red O stain on formalin fixed liver

rom: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dmborel Sent: Tuesday, 29 January 2013 6:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Oil red O stain on formalin fixed liver We have an autopsy on a young child. I have a wo

[Histonet] Oil red O stain on formalin fixed liver

We have an autopsy on a young child. I have a working diagnosis of possible Reye's syndrome and need an Oil Red O stain on a section of formalin fixed liver (not processed) to confirm microvesicular hepatic steatosis. Does anyone do this stain, and could I send the tissue or unstained slides f

RE: [Histonet] Oil Red O

Oil Red O slides will never "fade". The technique isn't like typical histochemical procedures where dye molecules bind chemically to tissue components. In Oil Red O staining there isn't any chemical binding that can be reversed. What you end up with is a solution of the dye in the fat droplets.

[Histonet] Oil Red O

Hi all, I am staining cryo sections of aorta for lipids with Oil Red O and mounting my slides with Mowiol. My question is how long will they last without fading and should they be kept in a folder in the fridge? Thanks for your thoughts, Daniela Daniela Bodemer Research Assistan

RE: [Histonet] Oil red O versus Sudan 4

onet-boun...@lists.utsouthwestern.edu] On Behalf Of Candice Smoots Sent: Thursday, 26 January 2012 3:21 AM To: Histonet Subject: [Histonet] Oil red O versus Sudan 4 Hi Histonetters     I am staining the plaques in aorta. I perfuse my animal with pbs before I biospy the aorta. I then pin the aorta down onto a

Re: [Histonet] Oil red O versus Sudan 4

That would be great!   Thanks I remain yours truely, Candice Camille From: Elizabeth Chlipala To: 'Candice Smoots' ; Histonet Sent: Wednesday, January 25, 2012 10:43 AM Subject: RE: [Histonet] Oil red O versus Sudan 4 Candice Most of the

Re: [Histonet] Oil red O versus Sudan 4

: Candice Smoots Sent: Wednesday, January 25, 2012 10:32 AM Subject: RE: [Histonet] Oil red O versus Sudan 4 I do oil red a lot.  You are doing it on frozen sections right? What are you coverslipping with? Need a few more details of the protocol and I might be able to help =) Sarah Goebel-Dysart

RE: [Histonet] Oil red O versus Sudan 4

n.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Candice Smoots Sent: Wednesday, January 25, 2012 9:21 AM To: Histonet Subject: [Histonet] Oil red O versus Sudan 4 Hi Histonetters I am staining the plaques in aorta. I perfuse my animal with pbs before I biospy the aorta. I then pi

[Histonet] Oil red O versus Sudan 4

Hi Histonetters     I am staining the plaques in aorta. I perfuse my animal with pbs before I biospy the aorta. I then pin the aorta down onto a wax plate and  then i stain the inside of the aorta for my plaques.   This seems to work well with the Sudan 4 but not so much with the Oil red o. The

[Histonet] Oil Red O for FS on Muscle

Few more things: 1. The mixed/working solution is good for 1-2 hours only. If you filter it for a long time, this might use the good working time and then you have trouble, because it just won't work well for you. I just poor the stain and the water in a glass cylinder and shake it. That'

[Histonet] Oil Red O for FS on Muscle

Hi Akemi, Order the stain from Electron Microscopy Sciences - cat # 26503-02. It comes as 250 ml solution and is a lot less expensive than some other sources. I guess you can probably get it through VWR. Mix 3 parts of this stock with 2 parts dist. Water and let it stand for 10 min. Filt

Re: [Histonet] Oil Red O for FS on Muscle

> > -Ursprüngliche Nachricht- > Von: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Akemi > Allison > Gesendet: Mittwoch, 26. Jänner 2011 18:16 > An: histonet > Betreff: [Histonet] Oil Red O for FS on Muscle >

AW: [Histonet] Oil Red O for FS on Muscle

-boun...@lists.utsouthwestern.edu] Im Auftrag von Akemi Allison Gesendet: Mittwoch, 26. Jänner 2011 18:16 An: histonet Betreff: [Histonet] Oil Red O for FS on Muscle Happy hump day! Does anyone have a good procedure for Oil Red O for FS on Muscle. The procedure the lab I am working with is having a great

Re: [Histonet] Oil Red O for FS on Muscle

I've done many of these stains and I get my ORO stain from Poly Scientific. Actually I use the whole kit and I use Freida's protocol in the second edition. That includes fixing in 37% formaldehyde. I filter the ORO just before use and usually get nice clean slides. If there is a residue it isn't

RE: [Histonet] Oil Red O for FS on Muscle

: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Wednesday, January 26, 2011 10:16 AM To: histonet Subject: [Histonet] Oil Red O for FS on

RE: [Histonet] Oil Red O for FS on Muscle

f Of Akemi Allison Sent: Wednesday, January 26, 2011 11:16 AM To: histonet Subject: [Histonet] Oil Red O for FS on Muscle Happy hump day! Does anyone have a good procedure for Oil Red O for FS on Muscle. The procedure the lab I am working with is having a great deal of problems with their mus

[Histonet] Oil Red O for FS on Muscle

Happy hump day! Does anyone have a good procedure for Oil Red O for FS on Muscle. The procedure the lab I am working with is having a great deal of problems with their muscle biopsy panel. I am trouble-shooting some of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase, I am wo

Re: [Histonet] Oil Red O

That's the way it normally looks, kinda globby. ORO is really not that soluble in fat, just more soluble than the prop. glycol. Jay A. Lundgren M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists

[Histonet] Oil Red O

Hi Everyone Posting this for a colleague who is doing oil red o staining on carotid plaques and is seeing both large globular and abundant fine small globular staining. Localization is correct but she is concerned about the amount and size variability of the staining pattern. She has been u

Re: [Histonet] Oil Red O control

ovember 10, 2010 9:45 AM To: "Geoff McAuliffe" ; "Finlay Finlay" Cc: Subject: [Histonet] Oil Red O control Hello - How does everyone handle having a positive control for Oil Red O - We very infrequently have this stain and have had difficulty keeping control on hand. Th

RE: [Histonet] Oil Red O control

Once you mix your stock solution with water, it is good for only about an hour. Then you have to discard it. Michelle Aloni MS HTL (ASCP) USC Keck School of Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwe

RE: [Histonet] Oil Red O control

McAuliffe; Finlay Finlay Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Oil Red O control Hello - How does everyone handle having a positive control for Oil Red O - We very infrequently have this stain and have had difficulty keeping control on hand. Thank you Anna

RE: [Histonet] Oil Red O control

[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Drew Meyer Sent: Wednesday, November 10, 2010 9:55 AM To: Inman, Anna Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Oil Red O control There was a discussion on this recently, so you may want to search the archives for it. In

Re: [Histonet] Oil Red O control

There was a discussion on this recently, so you may want to search the archives for it. In my opinion, the easiest and best control to use is Mayo. Just smear it on a slide as you need it and there you go. Cheap and easy. Drew On Wed, Nov 10, 2010 at 09:45, Inman, Anna wrote: > Hello - > > H

[Histonet] Oil Red O control

Hello - How does everyone handle having a positive control for Oil Red O - We very infrequently have this stain and have had difficulty keeping control on hand. Thank you Anna CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended r

Re: [Histonet] Oil Red O trivia

So, the pathologist does not know the answer but he wants to annoy trainees? Glad I don't work for him! Geoff Finlay Finlay wrote: Hello Just wondering if anyone knows the what the 'O' in oil red O stands for. I suspect that it stands for nothing other than O similar to the 'G' in Orange G.

[Histonet] Oil Red O trivia

Hello Just wondering if anyone knows the what the 'O' in oil red O stands for. I suspect that it stands for nothing other than O similar to the 'G' in Orange G. One of our pathologists is looking for a tricky stain question to annoy trainees with. Thanks for your help Finlay _

[Histonet] Oil Red O staining for atherosclerosis lesions

Hi, We stain frozen aortic root sections with oil red o (diluted in propylen glycol) as follow: 1. Fix slides in 10% formalin (5 min) 2. Rinse in 1X PBS (5 min) 3. Dip in Distilled H2O until clear (3 dips) 4. Transfer slides into 100% propylene glycol (2 min)

[Histonet] Oil Red O Staining

Hi, Other than using plus slides and air drying, what other techniques do you use to help frozen section tissues for Oil Red O to adhere to the slides? How long do you usually air dry slides for cryostat sectioning? Thanks in advance for your input. Sharon This

[Histonet] Oil Red O protocol

I¹m looking for a protocol for Oil Red O that does not use propylene glycol to make the Oil Red O. Does anyone have a protocol that I could use? Aprill Watanabe, B.S. Research Associate Integrated Cancer Genomics Division Tissue Microarray Center (TMA) Translational Genomics Research Institute (T

RE: [Histonet] Oil red o mounting media

We use Crystalmount. You don't even have to coverslip. Just make sure you evenly distribute the mountant on the slide and let it dry. We usually put it on a hot plate for a bit. This leaves a hardened shell around the tissue. The slides can be read like this too. If you really need a coverslip,

[Histonet] Oil red o mounting media

Hi, I will be staining many samples for Oil red O in the near future. I was wondering if there were any aqueous mounting medias out there that perform superior to Glycergel? Thanks Dave * This message and any attachments a

Re: [Histonet] Oil Red O despair - point of clarification

vouch for isopropyl as I used ethanol, but it is good to know there's an alternative in case we run out of triethylphosphate. Amos Brooks Message: 10 Date: Mon, 17 Nov 2008 11:56:45 -0600 From: "Michele Wich" <[EMAIL PROTECTED]> Subject: [Histonet] Oil Red O despair To: Messag

Re: [Histonet] Oil Red O despair

" <[EMAIL PROTECTED]> Subject: [Histonet] Oil Red O despair To: Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset="us-ascii" I'm wondering what are the pros (or cons) of using the propylene glycol version of Oil Red O over the isopropanol method.

[Histonet] Oil Red O despair

te on the slide.) I can't avouch for isopropyl as I used ethanol, but it is good to know there's an alternative in case we run out of triethylphosphate. Amos Brooks Message: 10 Date: Mon, 17 Nov 2008 11:56:45 -0600 From: "Michele Wich" <[EMAIL PROTECTED]> Subject:

Re: [Histonet] Oil Red O despair

Dear Michele, One of my first histology research papers was "Relative effectiveness of various solvents for oil red O" (Medical Laboratory Technology, 1974, 335-341.) It was concluded that propylene glycol solvents for oil red O produced poor contrasts. Isopropanol, as a solvent, can diss

RE: [Histonet] Oil Red O despair

Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Michele Wich Sent: Tuesday, 18 November 2008 4:57 AM To: histonet@lists.utsouthwestern.edu Subject: [

Re: [Histonet] Oil Red O despair

. -Original Message- From: Michele Wich <[EMAIL PROTECTED]> To: histonet@lists.utsouthwestern.edu Sent: Mon, 17 Nov 2008 11:56 am Subject: [Histonet] Oil Red O despair I'm wondering what are the pros (or cons) of using the propylene glycol version of Oil Red O over the

Re: [Histonet] Oil Red O despair

: Michele Wich <[EMAIL PROTECTED]> To: histonet@lists.utsouthwestern.edu Sent: Mon, 17 Nov 2008 11:56 am Subject: [Histonet] Oil Red O despair I'm wondering what are the pros (or cons) of using the propylene glycol version of Oil Red O over the isopropanol method. Is one more suited to

[Histonet] Oil Red O despair

I'm wondering what are the pros (or cons) of using the propylene glycol version of Oil Red O over the isopropanol method. Is one more suited to a specific application? I'm trying to stain a frozen section of liver, which one would suspect would be loaded with fat, and have thus far been unsuccessf