Diana,
I have used the HM355S at two different labs and liked it. Make sure to
demo it or purchase it with the “E” type disposable blade carrier. No one
liked the “ER” type blade carrier. You can see the difference between them
in the operator manual -?just do a browser search for the manual. I
: Thursday, February 29, 2024 3:50 AM
To: histonet@lists.utsouthwestern.edu; Diana Martinez-Longoria
Subject: Re: [Histonet] Question Regarding HM355S Automatic Microtome
Caution: This email came from outside Kaiser Permanente. Do not open
attachments or click on links if you do not recognize
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Question Regarding HM355S Automatic Microtome
[EXTERNAL MSG]
Hello All,
I am reaching out to seek help regarding if anyone is currently or have in the
past used the HM355S Automatic Microtome? What are the pros and cons?
Thank you
Hello All,
I am reaching out to seek help regarding if anyone is currently or have in the
past used the HM355S Automatic Microtome? What are the pros and cons?
Thank you in Advance!
Diana Martinez-Longoria
El Centro Regional Medical Center
Lead Histotechnician (ASCP)cm
Laboratory - Pathology
Friday, May 5, 2023 6:50:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Question about 72 hour upper fixation time for HER2
Hi Histonet,
Does anyone know if the 72 hour upper fixation time for HER2 was calculated
at room temperature? My lab is currently running our formalin at 40°c
Hi Histonet,
Does anyone know if the 72 hour upper fixation time for HER2 was calculated
at room temperature? My lab is currently running our formalin at 40°c and
we go 72 hours on the long weekends, so I'm worried it might be overfixed.
Thanks,
Tony
--
Tony Auge HTL (ASCP)CM QIHC
Cell: (651)
From: zzz_Tag_histonet-lists
Sent: Thursday, April 21, 2022 7:41 AM
To: zzz_Tag_histonet-lists
Subject: [Histonet] Question regarding posting job openings
Is it permitted for a company to post job openings in Histonet? If so, how does
that company go about posting it?
Lisa Freeman, AAS, HT
Histology Superv
Is it permitted for a company to post job openings in Histonet? If so, how does
that company go about posting it?
Lisa Freeman, AAS, HT
Histology Supervisor
Toxicologic Pathology Associates
National Center for Toxicological Research
3900 NCTR RD
Jefferson, AR 72079
Phone: 870-543-7234
E-mail:
So I am working with Canine femurs and tibias that have been dissected as
medial and lateral - they have been decaled in formic acid - rinsed in running
tap well - processed on a routine 1 hour processing program . cutting very
well!!
My issue is that the articular cartilage on the bone
Hi Richard!
That is the duplicate of what we do in our lab. We were putting both the
core and the clot on the same slide for in house testing, but den outs we
sometimes have to split the specimen and only on a slide.
I have a question for you about ISH Kappa/Lambda. What decal protocol do
you
Hello everyone:
I'm wonder what your protocol is for cutting formalin-fixed, paraffin-embedded
bone marrow core biopsy specimens? Do you cut a limited number of slides
upfront and then go back to the block if the pathologist requests histochemical
stains or immunohistochemical tests? We are
Subject: [Histonet] Question about accessioning outside consult cases
We are in the process of switching to AP Beaker and in the midst of the build.
My question involves how others are handling outside consult surgical and/or
cytology cases. Do you assign them an unique number that identifies them
du
Subject: [Histonet] Question about accessioning outside consult cases
We are in the process of switching to AP Beaker and in the midst of the build.
My question involves how others are handling outside consult surgical and/or
cytology cases. Do you assign them an unique number that ident
We identify our outside surgical consults as OC, for example OC20-00010
Les Raff
On Thu, Dec 10, 2020 at 12:45 PM Martha Ward-Pathology via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> We are in the process of switching to AP Beaker and in the midst of the
> build.My question
We are in the process of switching to AP Beaker and in the midst of the build.
My question involves how others are handling outside consult surgical and/or
cytology cases. Do you assign them an unique number that identifies them as a
consult as opposed to an inside case - SO20- verses
I was wondering if other facilities have the same issue as us?
We have excess breakage (brittleness) of the solution reservoirs (680 mL) for
our PRISMA stainer. We clean the dirty reservoirs in an automatic dishwasher
with a 18 minute dry cycle at 90 degrees.
How do other places clean
Hi
I have done several yrs of work on Eisenia fetida worms with a PhD student.
I decided to do a std Pwax processing schedule.
The student dissected the appropriate area then placed in 10% Formalin in PBS
pH7.4 for 24hrs on a rocker ( gentle agitation)
I then processed to Pwax using a std
I have crassicauda specimens ( kidney worms - relatively large worms 1-2 mm
width) And would like to know how you fix and process these for paraffin
sections of H slides.Do you do anything different for this?
Thanks
LeRoy Brown HT(ASCP) HTL
___
: Re: [Histonet] Question concerning H. pylori staining
Hi!
I found this instruction for a Hp- stain, that sounds similiar to yours.
They want the slides to be airdried after water-rinsing and before xylen.
But the result should be blue bacteria, not purple.
I would try to let the slides air-dry
with
Alcian yellow, to give a more contrasted result.
Gudrun Lang
-Ursprüngliche Nachricht-
Von: Bob Richmond via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Gesendet: Donnerstag, 9. April 2020 20:05
An: Histonet@lists.utsouthwestern.edu
Betreff: Re: [Histonet] Question concerning H
Michelle (where) asksi: >>We have a question about staining for H-Pylori
Using Quick Stain (Periodic acid 1%, Alician Yellow, Sodium Metabisulfate,
Toluidine Blue stock, Sodium Hydroxide) we notice what clearly looks like
the H-Pylori purple stained clusters, but after dehydration in 100% alcohol
Hi everyone! We have a question about staining for H-Pylori Using Quick Stain
(Periodic acid 1%, Alician Yellow, Sodium Metabisulfate,Toluidine Blue stock,
Sodium Hydroxide) we notice what clearly looks like the H-Pylori purple
stained clusters, but after dehydration in 100% alcohol the
Thank you Victoria
Regards
Ranna
Sent from my iPhone
> On Apr 8, 2020, at 4:39 AM, Victoria Baker wrote:
>
>
> Ranna,
>
> I took the course and it was well worth the cost for me. As to how it is
> assisting my career right now it isn't as my facility stopped using imaging
> about a
Ranna,
I took the course and it was well worth the cost for me. As to how it is
assisting my career right now it isn't as my facility stopped using imaging
about a year and a half ago. With the pandemic they may be rethinking
this, but it won't be immediate.
This type of skill will be more
Hi All,
I need inputs from my histo colleagues about Digital Pathology
certification from NSH. If anyone has done this program, how is it helping
in their career?
what are pros and cons? Do they include MatLab and R programming in
syllabus? I know it is very resourceful in research lab but what
2020 16:29
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Question about gelatin embedding
Hello,
I am here again. I am wondering if someone has good experience
embedding in gelatin-albumin for cryostat or vibratome sectioning.
Specifically we use brain tissue and is common in free
Hello,
I am here again. I am wondering if someone has good experience
embedding in gelatin-albumin for cryostat or vibratome sectioning.
Specifically we use brain tissue and is common in free floating techniques
non-attached parts of the same section float around and later that
generates all
We are preparing to install a Leica "Bond Stain Interface" which will allow us
to move away from having to print slide labels manually for our IHC slides. I
am not an expert on this so I am looking for help. We have "5" Bond IHC
instruments that will be hooked-up to the controller; however,
Hi Dr. Cartun,
It has been many years since I worked in EM but I my recollection is that
tissues could remain in 2% Glut indefinitely without detriment (for EM
purposes). However, Osmium tetroxide had to have a limited exposure.
Greg
--
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE C0A
How long can tissue remain in glutaraldehyde before EM testing is performed?
Thank you.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic
I am trying to find a way to obtain small bowel/intestine as a fresh/frozen
sample to be able to use it for a control with my staining for PGP9.5 free
floating IHC stain. Does anyone know where a good place would be to obtain
the needed control tissue?
*Haley Huggins, HT (ASCP)cm*
*Technical Lab
We are thinking of changing the way we report consult testing (IHC, Flow
Cytometry, and Molecular) for our system hospitals. Instead of accessioning
the consult case here, the requesting hospital would create a
"Procedure/Addendum" in Sunquest CoPath for their specimen and then send us the
...@colorado.edu]
Sent: Thursday, September 22, 2016 4:50 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Question on Sanderson Rapid Bone Stain
Hello everyone,
I am attempting to stain 30-40 micron thick undecalcified bone sections
embedded in poly(methyl methacrylate) with Sanderson
Hello everyone,
I am attempting to stain 30-40 micron thick undecalcified bone sections
embedded in poly(methyl methacrylate) with Sanderson Rapid Bone Stain (RBS)
with a Van Geison counter stain.
My first attempt at this stain resulted in a very faint stain from the RBS (I
could see very
Hi Tim,
In the hospitals I have worked, we used the PAD simply to communicate gross
pathologic diagnoses, so we would issue a gross description of the brain (like
the brain weight compared to normal, edema, atrophy, herniation, hemorrhage,
etc), "pending microscopic examination", and then
How do you handle counting days to preliminary autopsy dx (PAD) when doing
cases in which the brain autopsy results are the main component of disease? If
we accession at the time of autopsy and then let the brain fix for several days
before cutting in, then the PAD is many days past the 3-day
We will be switching from Cerner CoPath to Sunrise CoPath in the near future
(part of our Epic conversion). Is there anyone using Sunrise CoPath that uses
a prostate diagram in their report to demonstrate the absence or presence of
cancer in the different quadrants? Thank you.
Richard
When I was at NSH in Austin, I talked to a vendor with a very different type of
Histology. It was very soft and far less porous than the normal blue sponges.
Does anyone remember this or can you tell me where to get them? I believe the
company was from the UK with offices or distributors in
@lists.utsouthwestern.edu
Subject: [Histonet] Question re: accessory piece for tissue flotation bath
Morning!
Can anyone of you share the functionality of:
Flotation Work Station w/ 8" x 8" x 2 1/4" (Deep Dish), HistoOrientator and
Dryer
Curious if the HistoOrientator actually r
Morning!
Can anyone of you share the functionality of:
Flotation Work Station w/ 8" x 8" x 2 1/4" (Deep Dish), HistoOrientator and
Dryer
Curious if the HistoOrientator actually removes wrinkles as the description
states.
Thanks!
Jeanine H. Sanders, BS, HT (ASCP), QIHC
Centers for Disease
You can dewax absolutely safely using a 2% dishawasher soap solution at 90ºC
(twice) as washing in water.You can "dehydrate" stained stains by placing the
slides in an oven at 60ºC, also absolutely safely for the stained section.Under
separate cover I am sending articles on this subject.René
Hello
After decades of using standard organic solvents for paraffin- section
histology, I find that I've become highly allergic to the fumes. These include
commercial preps like Citrosolv and Hemo-De, as well as xylene and toluene,
which cause (in me) dizziness and a pounding headache. This
Good Morning!
Does anyone else use General Data for their barcode labeling system? If you
do, is your operating system the HTS system or Lab Cycle?
Thanks in advance!
Amanda Reichard, HTL (ASCP)cm
Histology Supervisor
Laboratory
Licking Memorial Health Systems
1320 W. Main St.
Newark, OH 43055
@lists.utsouthwestern.edu
Subject: [Histonet] Question
All,
I have a procedure here that call for and I quote 1.25 ml NaOH 10N in 1L of
water. I know how to get 1 N, but how do I get 10. Having rarely hd the
opportunity to make many Normal solutions ,my brain is not computing. Is it an
error?
Bernice
Bernice
...@lists.utsouthwestern.edu] On Behalf Of Bernice
Frederick
Sent: Thursday, April 30, 2015 1:35 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Question
All,
I have a procedure here that call for and I quote 1.25 ml NaOH 10N in 1L of
water. I know how to get 1 N, but how do I get 10. Having
The final concentration of 1.25 ml 10N NaOH into 1000 ml water is the same as:
12.5 ml 1N NaOH into 987.5 ml water.
-Original Message-
From: Geoff [mailto:mcaul...@rwjms.rutgers.edu]
Sent: Friday, May 01, 2015 9:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Question
All,
I have a procedure here that call for and I quote 1.25 ml NaOH 10N in 1L of
water. I know how to get 1 N, but how do I get 10. Having rarely hd the
opportunity to make many Normal solutions ,my brain is not computing. Is it an
error?
Bernice
Bernice Frederick HTL (ASCP)
Senior Research
Let me see if I have this straight:If a pathologist orders an Hpylori stain
on 2 blocks from the same specimen C1 and C2 we can only bill one 88342.
If this correct.Obviously if he ordered addition different IHC stains we
could change additional 88341's.
Jim Vickroy
Histology Manager
Hi Histonetters,
I have a question to ask if you don't mind. Can TRAP Histochemical staining be
performed after decalcifying with formic acid? Any tricks of the trade, etc?
If anyone has any experience or references that they could point me to, I would
greatly appreciate it. Thanks in
busy)
- Original Message -
From: Debra Siena dsi...@statlab.com
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, April 2, 2015 9:00:25 AM
Subject: [Histonet] Question about Formic acid decal and TRAP stain
Hi Histonetters,
I have a question to ask if you don't mind
Does anyone know if a Tzanck Smear falls under Virology or Cytology for POC
testing in a Dermatology clinic. I have heard conflicting theories. Thanks!
Caroline M. Pratt, MBA
Practice Administrator Dermpath
3020 Market Street, Ste 201
Philadelphia, PA 19104
Phone 215-349-8178
Cell
Cyto
Sent from my Verizon Wireless 4G LTE smartphone
Original message
From: Pratt, Caroline caroline.pr...@uphs.upenn.edu
Date:02/19/2015 4:59 PM (GMT-05:00)
To: 'histonet' histonet@lists.utsouthwestern.edu
Subject: [Histonet] Question about POC testing
Does anyone
Hi there,
we are having problems trying to cut some embedded samples (they crumble
in the bath and the few cuts we manage to get into HE are crap). These
are formalin fixed samples (bovine foetal and placenta samples) which
went straight from formlin into 100º ethanol for the dehydration
was the influence over tissues.
Thanks
Julio
Forwarded Message
Subject:RE: [EXTERNAL] [Histonet] Question regarding dehydration
Date: Fri, 13 Feb 2015 09:06:47 -0500
From: Roy, Ryan ryan@va.gov
To: 'Julio Benavides' j.benavi...@eae.csic.es
If the tissue is fatty
] On Behalf Of Julio Benavides
Sent: Friday, February 13, 2015 10:19 AM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] RE: [Histonet] Question regarding dehydration
Hi Ryan,
thanks a lot for your thoughts. These blocks were processed elsewhere and sent
to us for the cutting and staining
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Julio Benavides
Sent: Friday, February 13, 2015 8:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [EXTERNAL] RE: [Histonet] Question regarding dehydration
Thanks Ryan. Tissu sections where thin, something
Does anyone have a protocol to hold specimens for flow cytometry until the
pathologist examines a slide to confirm that it is
lymphoproliferative/lymphoma? We seem to be doing a lot of flow on
non-lymphoid specimens these days. Thanks.
Richard
Richard W. Cartun, MS, PhD
Director, Histology
All my histonet emails are coming thru my spam folder. Anyone else having this
problem? And how can I fix it?
Thanks in advance,
Sent from the iPad of Kim Tournear
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
NO, because the license in histotechnology INCLUDES high complexity testing.
On the other hand there are some tests like FISH with the VYSIS system that
requires a special training with its own certification.
René J.
On Tuesday, April 29, 2014 10:15 PM, Delia, Catherine deli...@uhnj.org
wrote:
Does anyone know if there is a regulatory agency that requires high complexity
testing certification for histotechnologists.
Catherine Susan Delia, BS. HT. ASCP
Chief Technologist, Anatomic Pathology
University Hospital
150 Bergen Street
E-151
Newark, New Jersey 07103
Phone: 973-972-5717
Cell:
Hi everyone,
I have been asked to post the following question regarding EM...
If you are providing EM services in your laboratory, who is performing the EM,
what is their job title and what are their job duties ( do they process, embed,
cut,stain,etc. or just certain portions ).
Any information
.
René J.
On Wed, 3/19/14, Gauch, Vicki gau...@mail.amc.edu wrote:
Subject: [Histonet] Question for Labs performing Electron Microscopy
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date: Wednesday, March 19, 2014, 10:25 AM
.
On Wed, 3/19/14, Gauch, Vicki gau...@mail.amc.edu wrote:
Subject: [Histonet] Question for Labs performing Electron Microscopy
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Date: Wednesday, March 19, 2014, 10:25 AM
Hi
Dear Histonet Colleagues,
I hope everyone is doing well--
We are going to be using a goat mandible model (embedded in MMA) for a project
and will need to administer tetracycline and/or calcein (others if suggested)
for histomorphometric analysis. Would anyone be kind enough to share their
Histonetters,
I was wondering how long a bone specimen (portion of pt's tibia in this case)
can be in a refrigerator before it will decompose and not produce a good
quality section? The case was completed at 3:30 pm, refrigerated and then put
into formalin at 8:15 am the next day? I am
Hi Peggy,
In my opinion, it depends upon the objective of the study. Cell autolysis
begins shortly after death as a result of lack of oxygen supply as well as
nutrients. The membranes of the lysosomes break down, and the acid hydrolases
begin to degrade the cellular
If you're merely looking
Hello -
Our research group does a fair amount of autoradiography with frozen sections
and we sometimes perform IHC or routine stains. I am not a histologist (nor do
we have one in our current group), so I assumed that the correct answer was
alcoholic formalin, because the other options were
and
Cytochemistry, vol. 33, No. 8. pp. 845-855. pdf available at
http://www.gofindpdf.com/readpdf/formaldehyde-fixation.html Joelle Weaver
MAOM, HTL (ASCP) QIHC
From: jone...@mir.wustl.edu
To: histonet@lists.utsouthwestern.edu
Date: Thu, 3 Oct 2013 19:32:51 +
CC:
Subject: [Histonet] Question about
Is anyone having an issue with Ventana's Pan cytokeratin? Please answer
offline.
Pam Marcum
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham,
Andrea L - (algranth)
Sent: Thursday, August 01, 2013 4:52 PM
Cc: HISTONET
Subject: Re: [Histonet] Question - OR specimens to Pathology
OR - you can get
How are other hospitals transporting fresh tissue specimens from operating
rooms to the Pathology Frozen Section room for an intraoperative consultation?
Occasionally, we get specimens delivered on towels and I don't think this is
appropriate. I think they should be in a container that can be
...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun
[rcar...@harthosp.org]
Sent: Thursday, August 01, 2013 2:26 PM
To: Histonet
Subject: [Histonet] Question - OR specimens to Pathology
How are other hospitals transporting fresh tissue specimens from operating
rooms to the Pathology Frozen Section room
OR - you can get a rubbermaid took box and slap a few biohazard stickers on it
and put some formalin pads inside and you have a transport box for surgical
specimens.
I happen to know that that is how this box was invented.
Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of
: Grantham, Andrea L - (algranth) algra...@email.arizona.edu
To:
Cc: HISTONET histonet@lists.utsouthwestern.edu
Sent: Thursday, August 1, 2013 3:51 PM
Subject: Re: [Histonet] Question - OR specimens to Pathology
OR - you can get a rubbermaid took box and slap a few biohazard stickers
Is anyone using a commercially available antibody (not RUO) for the
immunohistochemical detection of MDM in liposarcoma? Thank you.
Richard
Richard W. Cartun, MS, PhD
Director, Histology Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Howdy Folks,
As I am sure you can tell we are subscribers to histonet and appreciate the
generosity of members in helping solve the occasional problem we encounter.
We are in need of finding either an individual or lab in our area to perform
mammal root sectioning and slide preparation for the
out, vaccum and
rinse steps and air dry steps needed to decontaminate some ( usually older)
cryostats.
Joelle Weaver MAOM, HTL (ASCP) QIHC
From: abri...@brightinstruments.com
Date: Wed, 20 Mar 2013 21:37:45 +
To: marilyn.a.we...@kp.org
Subject: Re: [Histonet] question
CC
...@brightinstruments.com
Subject: Re: [Histonet] question
Date: Thu, 21 Mar 2013 10:25:41 +
To: joellewea...@hotmail.com
I do not see that as UV will not decontaminate areas and tissue that are not in
the UV light path or thick tissue.Alan Bright
Sent from my iPhone
On 20 Mar 2013, at 23:38, joelle
Thank you for your response about the squames and we are all wearing
gloves now, if one uses them then we all do is my motto, although in 50
years of cutting, this is the first time this has come up. . guess I was
lucky. anyway, the same pathologists would like to know if there is a
standard
Strange about the gloves when vacuuming out fresh tissue trimmings from
cryostats and exhausting the air back into the lab is done.
Alan Bright
On 20 Mar 2013, at 19:07, marilyn.a.we...@kp.org marilyn.a.we...@kp.org
wrote:
Thank you for your response about the squames and we are all wearing
To: marilyn.a.we...@kp.org
Subject: Re: [Histonet] question
CC: histonet@lists.utsouthwestern.edu
Strange about the gloves when vacuuming out fresh tissue trimmings from
cryostats and exhausting the air back into the lab is done.
Alan Bright
On 20 Mar 2013, at 19:07, marilyn.a.we...@kp.org
sorry for the misspelling in my previous post.
Joelle Weaver MAOM, HTL (ASCP) QIHC
From: joellewea...@hotmail.com
To: abri...@brightinstruments.com; marilyn.a.we...@kp.org
Date: Wed, 20 Mar 2013 23:38:04 +
Subject: RE: [Histonet] question
CC: histonet@lists.utsouthwestern.edu
I am asking this for a researcher. Please respond to her at
klar_elizab...@columbusstate.edumailto:klar_elizab...@columbusstate.edu. Her
name is Ely Klar.
She is looking for any form of estrogen antibody that works on bass fish
gonads. Vendor's responses welcome.
Shirley A. Powell,
We are using standard avidin / biotin staining with alkaline phosphatase
labeling and sometimes, not every time and not every slide in the same run
we get residual blotchy spots and streaking of the reaction on and off the
tissue. We have increased to two rinses after primary and two rinses after
Hi,
Can anyone with experience in cutting thick FFPE brain sections please give
advice on how to get a thick brain section without it curling up before it gets
to the waterbath? We are doing okay at cutting these at 10 microns, but are
trying to get some 12 micron sections and the section just
Hi Christina:
I use Paraplast to infiltrate the formalin fixed brain tissue, but I use
Surgipath Embedding Medium from Leica (Catalogue number 3801320) to
embed the tissue.
(Surgipath does have about the same melting point as the Paraplast)
Even the 10 micron sections (for the Congo red
I'm new to the Histonet website, but not new to histotechnology.
I'm trying to salvage some irreplaceable Zucker Diabetic Fatty rat tissue
samples that were embedded in paraffin in May 2009. They have sat on my bench
since they were embedded as we thought the entire experiment was a huge
Hi everyone,
Just have a quick question for all of you out there in
histo-land. Have you ever bought or worked with a gravity convection oven (the
thermo shandon 20GC), for drying of slides?? If so..how was it? Did it properly
dry all of your slides, did it keep temp?? I am
UP.
René J.
From: Natalie Nagy nagy_nata...@holyokehealth.com
To: histonet@lists.utsouthwestern.edu
Sent: Monday, January 28, 2013 12:40 PM
Subject: [Histonet] Question about slide drying/ convection ovens
Hi everyone,
Just have a quick question for all of you out
; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Question about slide drying/ convection ovens
Natalia:
I just cannot understand the concept of gravity convection oven because if
you say gravity by definition that will mean that the heat will work by
gravity and any heated air goes, also
Are IHC high complexity test.
Courtney Pierce
IHC Specialist
Quintiles
Translational RD - Oncology
Innovation
Navigating the new health
777 Oakmont Lane Suite 100
Westmont,IL 60559
Office: + 630-203-6234
courtney.pie...@quintiles.com
clinical | commercial | consulting | capital
Yes!
René J.
From: Courtney Pierce courtney.pie...@quintiles.com
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Tuesday, January 22, 2013 1:04 PM
Subject: [Histonet] Question
Are IHC high complexity test.
Courtney Pierce
IHC Specialist
Quintiles
Translational RD
The staining portion is not high complexity. The reading of the slide is.
On Tue, Jan 22, 2013 at 10:04 AM, Courtney Pierce
courtney.pie...@quintiles.com wrote:
Are IHC high complexity test.
Courtney Pierce
IHC Specialist
Quintiles
Translational RD - Oncology
Innovation
Navigating the
Hello,
How do I go about removing a question from this site? Thanks!
Chris
ENFD Supervisor
Bako Pathology Services
ch...@bakopathology.commailto:ch...@bakopathology.com
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
: [Histonet] Question removal
Hello,
How do I go about removing a question from this site? Thanks!
Chris
ENFD Supervisor
Bako Pathology Services
ch...@bakopathology.commailto:ch...@bakopathology.com
___
Histonet mailing list
Histonet
How does everyone handle storing extra paraffin sections that are cut as a
standard on certain protocols, such as prostate needle bx's? We currently
place them on a slide and save until the case is signed out. I am concerned
with the amount of waste and cost with the way we are doing it and
the protocols.
René J.
From: Webb, Dorothy L dorothy.l.w...@healthpartners.com
To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu
Sent: Friday, December 7, 2012 1:10 PM
Subject: [Histonet] question(s)
How does everyone handle storing extra paraffin sections that are cut as a
standard
Hi all,
I am performing IHC on tissue that was formalin fixed for 48 hours and then
embedded in OCT. I have been using Gold Plus slides from Thermo Fisher.I
am staining rat spleen and mandibular lymph nodes. With a peroxidase method,
the tissue completely digests off the slides as soon as
.
From: Thurby, Christina christina.thu...@bms.com
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Tuesday, November 20, 2012 10:03 AM
Subject: [Histonet] Question on frozen section IHC and tissue adhesion
Hi all,
I am performing IHC on tissue that was formalin fixed
Hi!
Can anyone tell me, if the amplification kit of Ventana also works on IgM
antibodies.
The specification sheet isn't very clear and I must admit, that I just don't
know, if the fc of the IgG and IgM of one species is the same.
In other words, if the anti-IgG in the amplifier also binds to
1 - 100 of 223 matches
Mail list logo
