@lists.utsouthwestern.edu
Subject: [Histonet] Re IHC help
Caution: This email came from outside Kaiser Permanente. Do not open
attachments or click on links if you do not recognize the sender.
__
Hi,
If HIER at 90 degrees C is causing
Hi,
If HIER at 90 degrees C is causing tissue lifting, you could try a lower
temperature for longer. I was recommended 60 degrees overnight for
bone/cartilage and have had far fewer problems with this gentler treatment.
Perhaps this would suit the skin samples too.
Best wishes,
Cath
If there is a cytology lab near you, they will take use of it.
--
Diese Nachricht wurde von meinem Android Mobiltelefon mit GMX Mail
gesendet.
Am 30.05.24, 01:21 schrieb Curt Tague via Histonet
:
My mistake, it’s methanol….
No bueno for tissue?
Curt
Get
Hi,
Direct IiF (a fluorescent conjugated primary) is certainly easier, but
there are some reasons one might prefer using an indirect method (using a
conjugated secondary). Indirect methods allow the use of a different
wavelength to be used simply by switching the secondary. It is also
Dear Alida,
That is a good point.
I probably have only done HOECHST on Pwax sections after they have been
heat-Antigen retrieved ( HIER)
However, H ( or DAPI) works on fixed cell monolayers: they don't get HIER
treatment)
Nor do frozen sections ( well, some do) and the HOECHST/DAPI works fine on
Use DAPI/HOECHST at the same concentration you use for cells/FS
I use Hoechst H33258 Sigma 10mg/ml soln
I dilute by adding 0.5 microL to 100 microL buffer then add 1/100 to Alexa
secondaries ( final 1/20K diln factor)
Some incubate in HOECHST or DAPI after secondary ab incubation, for IF
Sure, if
AM
To: Histonet Usegroup
Subject: [Histonet] Re-embed agar/FFPE tissue in plastic?
I have a bunch of precious FFPE samples that were embedded in agar prior to
being embedded in wax (so as to orient the tissue easily). The client now
wants these samples to be taken backwards out of wax
I have a bunch of precious FFPE samples that were embedded in agar prior to
being embedded in wax (so as to orient the tissue easily). The client now
wants these samples to be taken backwards out of wax and processed for
semithin plastic GMA (JB-4) sectioning.
Is that possible? Will the tissue
of Pathology
From: Lauren Sweeney via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Monday, February 11, 2019 3:03 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] re-processing help
Hello Histonet,
We have issues- please advise! Long story short
Hello Histonet,
We have issues- please advise! Long story short- some tissues did not get
processed over the weekend, they went through the process but were not
submerged in ANY (we think) of the reagents. Now they are shriveled up and
brittle (they are intestinal). Is there any way to save
PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re guinea pig IHC
Ms Ruegg, as usual , gives excellent advice: avoid HRP.
Use Alk phos?
Block end. alk phos by using levamisole.
However, Ms Shivers does not state the fixation status of her FS.
Is the Gpig tissue perfused-fixed
Ms Ruegg, as usual , gives excellent advice: avoid HRP.
Use Alk phos?
Block end. alk phos by using levamisole.
However, Ms Shivers does not state the fixation status of her FS.
Is the Gpig tissue perfused-fixed then frozen orfrozen as unfixed...then
fixed?
Alsowhy 0.3% H2O2?
Use
No pretreatments for anything that is not formalin fixed. I think 95% ETOH
for a fixative but but others may have a better idea than I.
Greg
--
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE C0A 1P0
*Everything in moderation...even moderation itself**!*
Hey Histonetter,
How can I re-attach the film with the tissue back to the blank slide? Have
some older slides and the slide film has popped off.
Renee H. Workman
Histology Supervisor
Virginia Urology
9105 Stony Point Drive
Richmond, VA 23235
W: 804-527-1316 | F: 804-270-0917
We had an unsuccessful embedding run, using Embed 812 resin (an Epon
replacement, from Electron Microscopy Services). After curing in the oven, the
resin blocks came out “gummy” (flexible), perhaps due to a carryover of water
in the paper labels. Would it be possible to re-embed the tissue,
Sorry about hitting send too soon.
Repeat of things to try:
a. Do not use regressive hematoxylin and eosin where hematoxylin
can be overly differentiated i.e. removed from too thin sections. Use
progressive hematoxylin i.e. Gill II or Gill III type formulation. DO NOT
use acid
I've been using the Dako coverslipper for over three years now. Once i had got
used to ensuring the sliding mechanism was always kept mountant free and
regularly changed the xylene in the u tube we have had very few problems with
this machine. Its footprint is without question the smallest
We use the Dako cover slipper and provided the moving arm is kept free from
dried mounting medium we have found this to be a terrific machine. It also has
a really small footprint and so fits nicely into our fume hood. Can do about
400 slides an hour and produces very few bubbles.
Steve Weston
It isn't very clear, but for the Formic Acid/sodium Citrate buffer you have
to use trisodium citrate. 10 g of disodium or monosodium citrate wouldn't
work as well...
--
Tyrone Genade
Orange City, Iowa
tel: (+1) 712 230 4101
http://tgenade.freeshell.org
Dorothy and Carl,
Comments about your Histonet replies on formic acid decalcification.
The Morse solution referred to by Dorothy can be picked up online by typing
in the DOI number: 10.1.1.4689.3439.pdf or title,Morse A. Formic
acid-sodium citrate decalcification and butyl alcohol
Mouse knee joints:
done lots of decalcified FFPWS for assessment of articular cartilage
degeneration models.
See Histonet images for a Tol blue image.
Decal in 10 % EDTA for 3 days on a rocker at RT.
Sure5days if you are worried.
No difference in Immuno-reactivity, imho.
If you want to use
Thank you, Gayle! This is exactly what I was looking for and we are willing to
make this in house. We are trying just for if acid and water, but the buffering
salt should be added. I will try de calcifying mouse knees next week with this
protocol. Thank you for the reference, I appreciate your
Merissa and Tim,
This formic acid decalcifying solution is basically the classic Evans and
Krajian fluid (Sheehan and Hrapchak, Theory and Practice of
Histotechnology, 2nd edition, P.92). Shandon has added other ingredients
for some reason, and has kept those concentrations proprietary.
I use the anti CD68 clone FA-11 ( obtained from Abcam).
It works very well on frozen sections but, I have been unable to get any
positivity on Pwax sections ( using +/- Citric acid pH6 HIER)
Carl
___
Histonet mailing list
-
From: Histology Technician [mailto:histology81...@att.net]
Sent: Wednesday, May 13, 2015 12:06 PM
To: kathy.mach...@lpnt.net; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] : Re: HE Stainer Question
Does anyone have a Thermo PrintMate that you'd like to share the SOP on?
Thanks
You wrote:
Is anyone of you familiar with the possibility of applying CD4, CD8 and CD68
antibodies on formalin fixed paraffin embedded liver of mice?
Thanks in advance.
Joost Bruijntjes
**
Thankfully and at long last after years of frustration by
...@bresnan.net
Sent: Wednesday, May 13, 2015 12:34 PM
To: Histonet
Subject: [Histonet] RE. murine CD4, CD8 and CD68 for FFPE tissue
You wrote:
Is anyone of you familiar with the possibility of applying CD4, CD8 and CD68
antibodies on formalin fixed paraffin embedded liver of mice?
Thanks
We just got rid of our Leica XL. It was not working right after about 10
years, little things started going wrong. And then it just quit reading the
white clip that we used for counterstaining IHC. The cost for someone to come
to the lab to fix it was ridiculous. The customer service from
Bernice, You have to buy the 10N solution. You can only dilute a given normal
solution, you cannot concentrate them.
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
Ouch!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard
Sent: Thursday, April 30, 2015 4:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC billing question
Effective
I have been following this with interest both now and in the past.
A word of caution about the acetone/ethanol fixation. I did NOT use the
acetone/alcohol fixative cold, but at RT (as it was taught to me by an IHC
expert). That is a bonus since you don't have to maintain A/A fixative in
a
Patrick,
We do a lot of frozen section IHC work. Years ago Gayle Callis turned me on to
fixing in cold acetone:ethanol (3:1) . We keep it at -20C and I fix for 10
min. on the bench then wash in PBS and proceed with the IHC. We do dry slides
for at least 30 min before fixing. This has worked
What type of tissue cassette is being used? What type of insert or wrap is
used. If one cassette processes correctly and the next to it does not, hard to
say tissue processor is causing issue.
Sounds like a water problem and it could be water trapped in cassette. Check
the rest of you process
We are having similar issues with our tissue.
Any troubleshooting insight would be greatly appreciated!
Thanks!
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Sue
[suetp...@comcast.net]
Sent:
In the past when using giemsa stain,
I came across two human cases of very long helicobacter organisms.. I was
stumped the first time since I had never seen one previously. I reflexed both
to immuno and both were positive with the h pylori antibody. I assume they were
both heilmani. I think it
Jim, yes, in my experience you are going to use this for 70 to 95% alcohol
steps, not 100, unless you get a water absorber (BR has one, at least used
to).
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San
: Garreyf garr...@gmail.com
To: Bob Richmond rsrichm...@gmail.com
Cc: Histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: H. Pylori Testing
Sent: Apr 29 '15 14:21
In the past when using giemsa stain,
I came across two human cases of very long
Helicobacter heilmannii (sorry I misspelled it before) was named
Gastrospirillum hominis when it was first described. Tight cylindrical
spirals, unlike the gull-wing (like you learned to draw birds in the sky
when you were in the fourth grade) morphology of H. pylori. I'm not sure
you could see
Nancy Stedman observes:
I believe IHC is more sensitive than the special stains too. One caveat
for anyone who works with veterinary samples - the H. pylori antibodies are
specific for H. pylori, so I have not found these antibodies to be helpful
for evaluating other species with
Thank you so much you to the ones who already replied to me! However I did not
receive any comment about the H1850 or H2250 from Energy Beam Science (EBS).
Does anyone have experience with any of these?
Thanks again in advance for a most appreciated prompt reply!
Liette Tougas, RT, B.Sc.,
Last chance to get on the bandwagon and join our Tri-State histology meeting
next week It promises to be a great meeting!! Go to
www.whs.wildapricot.org to sign up or see full brochure.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
From the pathologist's viewpoint, immunohistochemical stains for
Helicobacter are much faster to read than are the old dye methods such as
Giemsa.
Ventana claims that you can break even with one of their stainers if you
produce 600 billable slides a year (unless they've changed that number - a
We make our BAL cytospins directly--no centrifuging first.
Brian
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica
Sent: Tuesday, April 28, 2015 8:58 AM
To: histonet@lists.utsouthwestern.edu
Fixation of frozen sections in surgical pathology: about the only purpose
of the fixation step is to protect the hematoxylin from contamination.
Alcohol works fine for this purpose.
Bob Richmond
Samurai Pathologist
Maryville TN
___
Histonet mailing list
PM
To: Piche, Jessica; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Centrifuge for cytospins
We make our BAL cytospins directly--no centrifuging first.
Brian
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu
Of Bob Richmond
Sent: Tuesday, April 28, 2015 12:33 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: H. Pylori Testing
From the pathologist's viewpoint, immunohistochemical stains for
Helicobacter are much faster to read than are the old dye methods such as
Giemsa.
Ventana claims
The specific pages in the Dako Education Guide: Immunohistochemistry
Staining Methods, Fifth Edition are: Discussion on page 32 and
references on page 33. It's in the Fixation and Processing Chapter and
says no part of the process should have temperatures above 60C.
Rick Boen, BS, HTL
...@charter.net]
Sent: Sunday, April 26, 2015 10:42 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: IHC and Oven Temperatures
The specific pages in the Dako Education Guide: Immunohistochemistry
Staining Methods, Fifth Edition are: Discussion on page 32 and
references on page 33. It's
Thank you. I knew it was discussed in that reference. I guess my memory isn't
totally gone!
Joelle Weaver MAOM, HTL (ASCP) QIHC
Date: Sun, 26 Apr 2015 11:42:04 -0400
From: richardb...@charter.net
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: IHC and Oven
This seems like a classic case of drying of biopsies prior to fixation. This
can occur if biopsies are placed on absorbent paper (or on disinfecting alcohol
swabs, heaven forbid).
From: histonet-boun...@lists.utsouthwestern.edu
There is a grandfather clause if you were a histotech by april 24.1995. I
asked ClIA recently when they came to inspect me, as I fell into this category
but didn't know if you had to have the licence by then or just have been
performing high complexity test at that time (my licence was later
Gail,
The regulation is from CLIA '88. They list the requirements for that, and I
have never heard of a grandfather clause. I could be wrong about though.
NY state did not license HTL. I had a student who wanted to move there, and
did not because he was going to have an HTL, not HT. He would
:
Subject: [Histonet] RE: Question on IHC billing
Correct
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
This e-mail, including any attachments
Hi Chris,
You must go to http://lists.utsouthwestern.edu/mailman/listinfo/histonetand
sign yourself up..
Welcome!!!
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA
One pathologist said it looks like the tissue has been cooked. Which could
also be drying artifact after bx, before formalin.
The only issue is we can have two biopsies right next to one another in the
basket one looks good and one looks bad. My director also thinks it is the
processors.
find a problem with the processor.
Hope this help, Roberta
-Original Message-
From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu]
Sent: Wednesday, April 22, 2015 11:16 AM
To: Sue; Lisa Roy
Cc: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Nuclear Artifact
One pathologist said
we do not use freeze spray in the lab at all. this issue has really gotten me
bummed out since it is so sporadic. I am going to try to see if it is
associated to one day in particular. it is one PA but multiple techs have
identified the issue.
___
, Timothy' timothy.mor...@ucsf.edu, Sue
suetp...@comcast.net, Lisa Roy ro...@labcorp.com
Cc: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Date: 04/22/2015 11:32 AM
Subject:RE: [Histonet] RE: Nuclear Artifact
Sent by:histonet-boun
, Timothy';
Subject:RE: [Histonet] RE: Nuclear Artifact
Our pathologists also complained about some of the GI biopsies looking
burnt. We tracked all of the problem cases back to one histotech. The
histotech was causing the burn artifact with excessive use of freezing
spray.
From: Arbaugh
When I had this occur recently and sporadically, it was a collection issue.
Joelle Weaver MAOM, HTL (ASCP) QIHC
From: lbla...@digestivespecialists.com
To: ro...@labcorp.com; histonet@lists.utsouthwestern.edu
Date: Tue, 21 Apr 2015 13:48:07 -0400
CC:
Subject: [Histonet] RE
-I think you can use the other tissue controls.
Even CDC (Hansen's Disease Center) will send out mycobacterium leprae controls
free of charge, and I think they are armadillo.
The best control I have ever used for mast cells is canine mast cell tumors. I
request them from Vet Schools
I had this issue a couple of years back. Found out that there was a delay of
over an hour from the time the specimen was collected to the time it was being
put into fixative.
Tom
Tom Podawiltz HT (ASCP)
AP Section Head
LRGHealthcare
-Original Message-
From:
The first place I would look is to what may be happening before they reach me.
If it's only one site with an issue, it sounds more like an issue at collection.
Linda
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On
N
[tnma...@mdanderson.org]
Sent: Tuesday, April 21, 2015 2:12 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: (no subject)
-I think you can use the other tissue controls.
Even CDC (Hansen's Disease Center) will send out mycobacterium leprae controls
free of charge, and I think
The95 for HIER is in liquid, The 82in the oven is dry slides. While wet high
temps enhance HIER, It seems the high dry temp does harm epitopes (there is a
paper somewhere on this but I don't have access to it right now).
Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular
Date: Thu, 16 Apr 2015 14:35:59 +
CC:
Subject: [Histonet] RE: IHC Billing Question
We have not been charging for the negative control, assuming that it was just
a cost of doing business. I would be interested to hear if anyone has been
charging for their negative controls as well
We recently added HER2 IHC testing in our lab which we are required to use a
negative reagent control
For each case. Is there a cpt code for negative reagent control reimbursement?
Any information on this
Would be much appreciated!
Thanks
Brandy Burnett
Histotechnoligist, QIHC(ASCP)
CCH
Moreira'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: IHC Billing Question
We recently added HER2 IHC testing in our lab which we are required to use a
negative reagent control For each case. Is there a cpt code for negative
reagent control reimbursement? Any information on this Would
I have never heard of anyone billing for a negative control and would
highly recommend against doing so. As mentioned, it is a cost of doing
business--which is why when CAP clarified that polymer detection systems
did not require them, most cost-conscious labs quit running them.
Regards,
Bryan
Brandy Joana
Controls are never billable, a control test does not produce a usable result
based on the patient's specimen. The results of control tests - positive or
negative only tell you that your reagents, stains, antibodies etc. are
performing as expected.
| Dawn Hanson | Lab Manager |
] On Behalf Of David Wright
Sent: Wednesday, April 15, 2015 2:06 PM
To: histonet@lists.utsouthwestern.edu
Cc: Yves Heremans
Subject: [Histonet] RE: perfusion stiffness
Hi Yves Histonet
It is certainly a good sign if limbs etc are stiff after perfusion, but maybe
not a guarantee that the target organ
Usually with the fatty tissues, I pick them up on superfrost slides and let it
air dry for 2-3 days at room temperature and then perform the ORO stains. So
far they seem to stay on.
Linda Prasad | Senior Scientist | Histopathology
t: (02) 9845 3306 | f: (02) 9845 3318 | e:
] on behalf of Linda Prasad (SCHN)
[linda.pra...@health.nsw.gov.au]
Sent: Wednesday, April 15, 2015 5:07 PM
To: 'Jo-Ann Bader, Ms.'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: ORO tissue falling off
Usually with the fatty tissues, I pick them up on superfrost slides and let it
air dry
(SCHN)
Cc: Jo-Ann Bader, Ms.; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: ORO tissue falling off
+1 to Linda, but I have found no difference on overnight vs multiple days.
Fatty liver is hard to do on all counts! It is tough enough sometimes to get a
decent section on the slide
+1 to Linda, but I have found no difference on overnight vs multiple days.
Fatty liver is hard to do on all counts! It is tough enough sometimes to get a
decent section on the slide.
Thanks for the other suggestions, certainly something I would try in the future
Yours
Caroline
Caroline Miller
Hi Yves Histonet
It is certainly a good sign if limbs etc are stiff after perfusion, but maybe
not a guarantee that the target organ is perfect given the short perfusions you
describe. Definitely, if I don't see stiffness I worry, check for a torn aortic
arch (you are doing it transcardially,
Hi
Good day to you,
I am writing this email to check if you have got the below email and have
any update on the same.
Thanks and I will look forward to your response
Best Regards
Emily
From: Emily Jacob [mailto:emily.ja...@crystaldatalist.com]
Sent: Monday, March 30,
Hi
Good day to you,
I am writing this email to check if you have got the below email and have
any update on the same.
Thanks and I will look forward to your response
Best Regards
Cynthia
From: Cynthia Hunter [mailto:cynthia.hun...@crystaldatalist.com]
Sent: Monday,
Hello Histonet!
Thank you all for your time and wonderful suggestions. This is the very
first time, I have to work with paraffin embedded brain tissues.
Based on the suggestions from Histonet colleagues, I have decided to
obtain 200 um thick brain sections for processing and embedding. Since I
She can be here in two weeks.
Sent from my iPhone
On Apr 12, 2015, at 11:01 AM, histonet-requ...@lists.utsouthwestern.edu
histonet-requ...@lists.utsouthwestern.edu wrote:
Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu
To subscribe or unsubscribe via the
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Catherine
Simonson
Sent: Friday, April 10, 2015 10:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: whole mouse brains
Hey there!
I embed whole
1. 30% H2O2 5 min. under movement.
2. wash in water 2x
3. 5% Acetic acid 1 min.
4. wash in water 2x
5. solution 1 20 min.
6. wash in water 2x
7. solution 2, piece by piece
8. wash in water and dry.
Solution 1: 1 part Azur II (1%) + 1 part Methylen blue (1%) + 2 parts
Na2CO3 (1%).
Solution 2:
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of hymclab
Sent: Monday, March 02, 2015 11:07 AM
To: 'Teri Johnson'; colleen_herr...@bshsi.org
Cc: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet]
I am not sure about the color change you are referencing, unless it is the
change that occurs with potassium permanganate filters, which turn black when
exhausted.
We have a countertop gross station with two Potassium Permanganate filters that
we change every 6 months. Keep in mind we do
Is anyone out there looking for an extra Ventana Benchmark Ultra for
IHC/ISH? I have one in good shape that is not being used anymore and I am
looking to free up some space. Email me if you are interested and I'll give
you more info on it.
Jeff Mack
dermack...@gmail.com
On Tue, Mar 17, 2015 at
I found several pages from Hayat's book Stains and Cytochemical Staining
1993 (for EM) with original authors cited after a Google search, key words
Laczko and Levai staining protocol. Go to this long link and read on hints
to improve staining plus the protocol.
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gayle Callis
Sent: Tuesday, April 07, 2015 2:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Pam Marcum colleague losing bone sections from slides
From Pam: I am currently trying to stain L6 vertebrae from
-Original Message-
From: Carol Fields
Sent: Tuesday, April 07, 2015 3:37 PM
To: 'Cooper, Brian'
Cc: 'histonet-boun...@lists.utsouthwestern.edu'
Subject: RE: [Histonet] Re: Pam Marcum colleague losing bone sections from
slides
I've used that also many times. It really does work.
Carole
From Pam: I am currently trying to stain L6 vertebrae from rabbits. They
have been decalcified and paraffin processed properly. I've tried cutting at
both 5 and 10 microns and my tissue is still not sticking to my slides. I
know my sectioning is fine because I'm successful with every other tissue
Wanda Platt Jones
On Apr 5, 2015, at 12:12 PM, histonet-requ...@lists.utsouthwestern.edu wrote:
Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
I've been in pathology over 50 years, and I never heard of anybody trying
to cut a section of a kidney stone. You sure this wasn't an April Fool grab?
Stone analysis - once done chemically, now done mostly by physical methods
- is of course clinically quite useful and is very often ordered. There
@lists.utsouthwestern.edu
Subject: [Histonet] RE: cleaning glassware
I always used to rinse the containers I used with alcohol and let them dry
before doing the stain. Some people use an acid alcohol rinse. Anne
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
I work in a small veterinary lab and when I do the Warthin Starry stain I mix
all the reagents in never used before disposable plastic beakers and I stain
the slides in an un-used slide mailer.
Roberta Horner
Animal Diagnostic Lab
Penn State University
-Original Message-
From:
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roberta Horner
Sent: Thursday, April 02, 2015 2:36 PM
To: Jeff Halstead; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: cleaning glassware
I work
The reference within a reference from Ray is A Chimeric Form of
Osteoprotegerin Inhibits Hypercalcemia and Bone Resorption Induced by IL-1β,
TNF-α, PTH, PTHrP, and 1,25(OH)2D3 . Sean Morony et al . J Bone Mineral
Res V 14, pp 1478-1485.
However, the formic acid decalcification method is
of equipment.
Ray
Washington
- Original Message -
From: Gayle Callis gayle.cal...@bresnan.net
To: Histonet histonet@lists.utsouthwestern.edu
Sent: Thursday, April 2, 2015 3:12:55 PM
Subject: [Histonet] Re: TRAP staining on formic acid decalcified bone
reference
The reference within
I always used to rinse the containers I used with alcohol and let them dry
before doing the stain. Some people use an acid alcohol rinse. Anne
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeff
Debbie
In our hands it has not worked on formic acid decaled samples, EDTA decal only.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com
March
Hi Julie,
Try increasing your deparaffinization times, sometimes there is just not enough
time in xylene so subsequent staining can be very inconsistent like described.
Kari Kienitz HT, (ASCP)
Histology Laboratory
Gastroenterology-EAST
The Oregon Clinic
NE 99th Ave
Portland, OR 97220
Julie,
Are the inconsistencies consistent among different sections? In other words,
with additional sections, are you seeing the paler areas in the same places or
different ones? If you section it today, are the pale areas in the same place
as when you sectioned it previously?
If it is the
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