[Histonet] Cryostat Shopping

2022-11-22 Thread Chris England via Histonet
Aloha Histonetters--I've missed you! 1. It's been a very long time since I last posted here so please forgive my heap of ignorance: I haven't done this in a hot minute. 2. I think I'm in the market for a cryostat! But it's been 20+ years since the last one I bought, and I have no clue what

Re: [Histonet] Cryostat anti-roll plate

2022-05-25 Thread Bob Richmond via Histonet
> > > My experience with anti-roll plates is mostly with the old International > cryostats of days gone by. Pathologists usually disdained anti-roll plates > and used an artist's brush to keep the section from rolling, but more > proficient users depended on them. I learned to use them in a researc

Re: [Histonet] Cryostat anti-roll plate

2022-05-25 Thread Keyser, Geri L via Histonet
insky ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cryostat anti-roll plate Maybe there's a product that increases friction with screw threads, doesn't have toxic outgassing, and works at low temperatures. I'm going to look for something like this. Geri -Original Mes

Re: [Histonet] Cryostat anti-roll plate

2022-05-25 Thread Keyser, Geri L via Histonet
8:32 AM To: Ken Marzinsky ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cryostat anti-roll plate WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. We ha

Re: [Histonet] Cryostat anti-roll plate

2022-05-25 Thread Anne Murvosh via Histonet
adjusting. Thanks Anne -Original Message- From: Ken Marzinsky via Histonet Sent: Tuesday, May 24, 2022 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat anti-roll plate Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? Does it work

Re: [Histonet] Cryostat anti-roll plate

2022-05-25 Thread Keyser, Geri L via Histonet
ay, May 24, 2022 3:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat anti-roll plate WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. H

[Histonet] Cryostat anti-roll plate

2022-05-24 Thread Ken Marzinsky via Histonet
Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? Does it work well on the Leicas and Microms, or do you have to figit with it a lot? Does anyone have any tricks they can share to get it to work all the time? I found that a little spritz of freeze spray on the plate

[Histonet] Cryostat cutting head temperatures

2022-05-19 Thread Ken Marzinsky via Histonet
I was asking about the cutting head temperature because the Leica CM3050 literature says the cutting head can be adjusted down to -50c separately from the chamber temperature. Does anyone out there use this unit? It seems to be very popular with the research community. Do you ever adjust the te

[Histonet] Cryostat vacuums

2021-08-30 Thread Wooten, Jennifer via Histonet
Does anyone use a vacuum to clean out their cryostats, either on or off-board? Jennifer Wooten, BA, BS, HTL (ASCP)CM Technical Supervisor | Anatomic Pathology | University Hospital IMPORTANT: This message originates from TriCore Reference Laboratories or one of its affiliate organizations or rep

Re: [Histonet] Cryostat validation

2021-08-24 Thread Terri Braud via Histonet
Tony - We were on the same page. We purchased fresh beef liver, sectioned and froze it, cut and stained duplicate blocks and had the pathologist sign off on the process. We were also able to get some tonsils delivered fresh and did them, too. Terri Terri L. Braud, HT(ASCP) HNL Laboratories f

Re: [Histonet] Cryostat

2021-08-10 Thread Colleen Forster via Histonet
Huntingdon Pike > Meadowbrook, PA 19046 > Ph: 215-938-3689 > Fax: 215-938-3874 > Today's Topics: > > 1. cryostat (Histology) > > Date: Mon, 9 Aug 2021 13:00:30 + > Subject: [Histonet] cryostat > Hello, > Trying to get everyone's opinion on cry

Re: [Histonet] Cryostat

2021-08-10 Thread Terri Braud via Histonet
s for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-3874 Today's Topics: 1. cryostat (Histology) Date: Mon, 9 Aug 2021 13:00:30 + Subject: [Histonet] cryostat Hello, Trying to get everyone's opinion on cryostats. We have had the Leica

Re: [Histonet] cryostat

2021-08-09 Thread Haiyan via Histonet
of Pathology > UC San Francisco Medical Center > > > -Original Message- > From: Histology via Histonet > Sent: Monday, August 09, 2021 6:01 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] cryostat > > Hello, > > Trying to get everyone'

Re: [Histonet] cryostat

2021-08-09 Thread Morken, Timothy via Histonet
enter -Original Message- From: Histology via Histonet Sent: Monday, August 09, 2021 6:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat Hello, Trying to get everyone's opinion on cryostats. We have had the Leica CM1850 forever and it is not being supported by Lei

[Histonet] cryostat

2021-08-09 Thread Histology via Histonet
Hello, Trying to get everyone's opinion on cryostats. We have had the Leica CM1850 forever and it is not being supported by Leica anymore. Thinking of trying a different company maybe Tanner or Rankin. What does everyone use and like. We do not cut Mohs, only DIFs. Maybe 1 a day. Thanks t

Re: [Histonet] Cryostat

2021-05-20 Thread MARR, Penelope (EAST SUSSEX HEALTHCARE NHS TRUST) via Histonet
7 7RD penny.m...@nhs.net 0300 131 4500 ext 734914 0300 131 4914 -Original Message- From: Brittany Hethcox [mailto:bheth...@hcmhcares.org] Sent: 19 May 2021 15:21 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat Hello everyone! I am new to the Histology world. You al

Re: [Histonet] Cryostat

2021-05-19 Thread Morken, Timothy via Histonet
-Original Message- From: Morken, Timothy via Histonet Sent: Wednesday, May 19, 2021 8:26 AM To: Brittany Hethcox Cc: Histonet Subject: Re: [Histonet] Cryostat Brittany, we have two of the Epredia Cryostar NX70 and love them. They 're reliable and easy to use. We have the elevator on ours a

Re: [Histonet] Cryostat

2021-05-19 Thread Morken, Timothy via Histonet
ssage- From: Brittany Hethcox via Histonet Sent: Wednesday, May 19, 2021 7:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat Hello everyone! I am new to the Histology world. You all may know my previous coworker, Rhonda Ford. She officially retired and I am so happy fo

[Histonet] Cryostat

2021-05-19 Thread Brittany Hethcox via Histonet
Hello everyone! I am new to the Histology world. You all may know my previous coworker, Rhonda Ford. She officially retired and I am so happy for her! With that being said, I am in a pickle because the cryostat we had completely broke down. We were having issues with it during my training period,

[Histonet] Cryostat Samples for Molecular Testing

2019-12-12 Thread Cooper, Brian via Histonet
Good afternoon Histonet! We would like to know what decontamination procedures you are performing to prevent cross-contamination in between cutting scrolls for frozen samples for molecular testing. We've had a few complaints of trace carryover from one sample to the next. Thanks, Brian D. Co

Re: [Histonet] Cryostat cooling time

2019-09-05 Thread Lynn Whitmore via Histonet
>required to wear a respirator such as N95 or even a PAP-R? > >Any thoughts are appreciated. > >Barb Moe >Histology Lab >Gundersen Health Systems >La Crosse WI >ba...@gundersenhealth.org<mailto:ba...@gundersenhealth.org> > > >-

[Histonet] Cryostat to room temperature

2019-09-04 Thread Moe, Barbi A via Histonet
Does anyone know how long it would take a cryostat that is maintained at approx. -22 degrees C to come to room temperature after being turned off? Any thoughts are appreciated. Barb Moe Histology Lab Gundersen Health Systems La Crosse WI ba...@gundersenhealth.org

[Histonet] cryostat vs sliding microtome

2019-07-08 Thread Emily Stevenson via Histonet
Hello, Looking for information on using a cryostat vs a sliding microtome for ENFD/small nerve fiber tissue cutting. Thanks! -- -- Confidentiality Notice: The information included in this e-mail, including any attachments, is for the sole use of the intended recipient and may contain informat

[Histonet] Cryostat question

2017-10-25 Thread Charles Riley via Histonet
Does anyone out there use the Avantik QS12 cryostats? We have a machine that is only a year and a half old and have had to get the Peltier system (the cryobar) replaced 4 times. Is anyone else having this problem consistently? Sent from my iPhone ___

[Histonet] Cryostat and Parts

2017-05-02 Thread Travis Troyer via Histonet
We are in the process of moving and we have some equipment and supplies that we are not taking with us.We have 2 Microm HM 505E cryostats and all of the internal components.One of the cryostats doesn't cool down and the other one doesn't advance properly but is otherwise in working order.

Re: [Histonet] cryostat decontamination

2016-08-15 Thread Duddey, Aimee via Histonet
wing their guidelines, you should be in compliance. Aimee -Original Message- From: Nancy Schmitt via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Monday, August 15, 2016 9:58 AM To: ' Subject: [Histonet] cryostat decontamination ANP.23410 states that cryostat is defrosted

[Histonet] cryostat decontamination

2016-08-15 Thread Nancy Schmitt via Histonet
ANP.23410 states that cryostat is defrosted and wiped down at regular intervals. What if you have UV decontam? Is there a ruling on that? Thanks Nancy Schmitt MLT, HT(ASCP) Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the in

[Histonet] Cryostat sectioning of frozen leaves

2016-08-09 Thread Jo-Ann Bader, Ms. via Histonet
I am trying to cut frozen leaf sections at 6 and 8 microns. All I am getting is bunched up leaf. What is the trick/ I am cutting at -20 should be cutting at a lower temp and cutting thicker? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312

[Histonet] Cryostat decontamination ANP23410

2016-07-11 Thread Piche, Jessica via Histonet
Hi Everyone, Just a quick question. If you defrost your cryostat to room temperature and wipe it out with 70% alcohol is that considered decontamination? Or does it have to be fumigated with formaldehyde, or have a UV function on board the machine? Thanks, Jessica Piche, HT(ASCP) Waterbury Hospi

Re: [Histonet] Cryostat Chirp

2016-05-03 Thread Elizabeth Chlipala via Histonet
03, 2016 2:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat Chirp My Leica CM 1850 cryostat is making a chirping sound and I can't figure out what is doing this. Has anyone experienced this sound, if what was the resolve. Thanks

[Histonet] Cryostat Chirp

2016-05-03 Thread Martin, Gary via Histonet
My Leica CM 1850 cryostat is making a chirping sound and I can't figure out what is doing this. Has anyone experienced this sound, if what was the resolve. Thanks Gary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwe

Re: [Histonet] Cryostat with UV disinfection

2016-03-14 Thread Pfaff, Karen via Histonet
: Friday, March 11, 2016 12:52 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Cryostat with UV disinfection For those of you that are CAP accredited, and use a cryostat with UV disinfection, how do you get around the CAP requirement listed below? We were told the UV di

Re: [Histonet] Cryostat with UV disinfection

2016-03-11 Thread Terri Braud via Histonet
For those of you that are CAP accredited, and use a cryostat with UV disinfection, how do you get around the CAP requirement listed below? We were told the UV disinfection did not replace wiping down with a tuberculocidal disinfectant at room temp. Do you have an approved tuberculocidal disinfec

[Histonet] cryostat

2016-03-10 Thread Silvia Bonner via Histonet
Hi Everyone, Can you recommend someone for preventative maintenance on cryostats? We have both Shandon and Leica cryostats. Thanks, Silvia Bonner, HT(ASCP) CM Histology Supervisor sbon...@pathregional.com Pathologists' Regional Laboratory 1225 Highland Ave Cla

[Histonet] Cryostat sectioning of FF lymph node

2015-12-14 Thread Martin, Erin via Histonet
Good morning! My pathologists would like us to cut formalin fixed (not yet processed) tonsil tissue on a cryostat for DIF staining. Has anyone done this? I did a quick search that seemed to indicate that it was possible but that the architecture of the cells would be altered because of ice cr

[Histonet] Cryostat vacuum

2015-09-11 Thread Morken, Timothy via Histonet
I just bought one from IMEB : http://www.imebinc.com/ Hello Histonet, Does anyone know where I can find a cryostat vacuum? I've used them in the past but can't seem to find a manufacturer. Thanks! Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General

[Histonet] Cryostat vacuum

2015-09-09 Thread Dessoye, Michael via Histonet
Hello Histonet, Does anyone know where I can find a cryostat vacuum? I've used them in the past but can't seem to find a manufacturer. Thanks! Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@comm

[Histonet] Cryostat purchasing

2015-02-11 Thread Lewis, Patrick
Hi Everyone, Can anyone recommend a good Cryostat to buy for use with Human Tissues. I'd like to look for a reasonably priced model. Also, I'd consider paying extra for a model that is designed for human tissues so it has disinfection ability or is easier to disinfect than older models. I'd als

[Histonet] Cryostat maintenance personnel

2015-01-21 Thread Lewis, Patrick
Hi everyone, Does anyone know who I would contact about servicing an old CM3000 Cryostat. This would be in the Seattle area and we have no service contract. I am going to call Leica tomorrow, but if anyone has information about repair/servicing that would be helpful. Thanks Patrick. CONFID

Re: [Histonet] Cryostat troublshooting. CM3050

2015-01-07 Thread Hans B Snyder
Hello Patrick, We have this same cryostat except it's the CM 3050S. Ours also has this issue usually after it's been running for many months with a defrost. The typical cause of your problem is the cylinder on which the block holder is attached has ice crystals or is "frozen". I feel the cause

[Histonet] Cryostat troublshooting. CM3050

2015-01-07 Thread Lewis, Patrick
H everyone, We have been using this old CM3050 cryostat for our Frozen OCT blocks. I am cutting at 5 uM. My issue is that when I use the hand rotator to cut my sections it doesn't seem to advance with each rotation. It can take as many as 4 rotations to go from one section to another. What w

[Histonet] CRYOSTAT RECOMMENDATIONSRE: Histonet Digest, Vol 125, Issue 24

2014-04-21 Thread Karen Kay
Good ;Morning, We have the Leica 1950 in our Laboratory and are extremely pleased with the insturment. Karen J Kay, MLT Supervisor - Histopathology and Cytology Laboratory Chinook Regional Hospital - South Zone West - Alberta Health Services Lethbridge, Alberta, Canada karen@albertahealthser

Re: [Histonet] cryostat design

2014-03-05 Thread Alan Bright
Dear Kim, Yes the Starlet cryostat made by Bright Instrument Company, it is in a small bench level cabinet as there is no other way of achieving good results on the refrigeration. Apart from freezing microtones which were highly inefficient on retrieving good quality thin sections. Hope this as

[Histonet] cryostat design

2014-03-05 Thread Kolman, Kimberly D.
Hello all; Is anyone aware of a cryostat design in which the microtome cabinet would sit at bench level? In other words, it would function like any regular bench microtome but be housed in a cold cabinet like a cryostat? This would enable the operator to work in a desk position instead of uncomfo

[Histonet] Cryostat Disinfection

2014-01-30 Thread Vickroy, Jim
In the CAP checklist it states that The cryostat must be4 defrosted and decontaminated by wiping all exposed surfaces with a tuberculocidal disinfectant. The cryostat should be at room temperature during decontamination unless otherwise specified by the manufacturer. The newest cryostat by

Re: [Histonet] Cryostat

2014-01-17 Thread Hugh Luk
cold cryostat with 100% alcohol. Hugh Hawaii -- Date: Thu, 16 Jan 2014 10:43:09 -0800 (PST) From: Madeline Gi Subject: Re: [Histonet] Cryostat To: "Abbott, Tanya" , "Histonet@lists.utsouthwestern.edu" Message-ID: <1389897789.16602.yahoomai

Re: [Histonet] Cryostat

2014-01-16 Thread Madeline Gi
I use to decon my cryostat with 100% Alco this keeps it from freezing up and it clean it.  best of luck.    Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelin...@yahoo.com On Wednesday, January 15, 2014 2:52 PM, "Abbott, Tanya" wrote:

[Histonet] Cryostat

2014-01-15 Thread Abbott, Tanya
Need to decon my cryostat Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This electronic mail and any attached documents are intended solely for the named

[Histonet] Cryostat Decontamination

2014-01-15 Thread Abbott, Tanya
I am looking for a procedure and product to use for decontamination after a potential TB case, help please! Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net

[Histonet] cryostat

2014-01-02 Thread Webb, Dorothy L
Does anyone have any current information on the cryostats with UV for decontamination? Specifically, is it accepted as a means of decontamination by CAP or JS or any other regulatory agencies? Basically, is it worth the extra dollars to go that route? Much thanks and Happy 2014!! Dorothy Web

RE: [Histonet] cryostat service

2013-10-28 Thread Walter Benton
; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryostat service Avantik is located in NJ and services cryostats in addition to microtomes, processors and stainers. www.avantik-us.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:his

RE: [Histonet] cryostat service

2013-10-28 Thread Rathborne, Toni
, 2013 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat service Hi everyone, I am interested in finding out if there is anyone in or close to Delaware that services and repairs cryostats. Any recommendations would be much appreciated. Thanks, Jean -- Jean Ross Delaware

RE: [Histonet] cryostat service

2013-10-28 Thread Barbara Tibbs
-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Jean Ross Sent: Monday, October 28, 2013 4:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat service Hi everyone, I am interested in finding out

[Histonet] cryostat service

2013-10-28 Thread Jean Ross
Hi everyone, I am interested in finding out if there is anyone in or close to Delaware that services and repairs cryostats. Any recommendations would be much appreciated. Thanks, Jean -- Jean Ross Delaware Biotechnology Institute, BioImaging Center University of Delaware 15 Innovation Way Suite

[Histonet] Cryostat disinfection

2013-08-17 Thread Cynthia Baranowski
In my previous email regarding weekly cryostat disinfection-- I meant to say that we clean with 70% alcohol, then bring to room temp, disinfect with Cavicide, wipe down with abs alcohol, bring back down to -22C. We also use 70% alcohol for our routine daily cleaning. Cindy Baranowski Emory Healt

[Histonet] Cryostat cleaning

2013-08-17 Thread Cynthia Baranowski
The only method acceptable to CAP is bringing the cryostat to room temp and disinfecting weekly UV light will not be an acceptable alternative by CAP. We clean out all tissue debris, wipe down with abs alcohol, bring cryostat to room temp, use Cavicide to disinfect, wipe down again with abs alcoh

RE: [Histonet] Cryostat cleaning

2013-08-16 Thread Hannen, Valerie
August 16, 2013 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat cleaning ANP.12087 states that the cryostat needs to be defrosted and wiped clean with a tuberculocidal. Can someone tell me what they use that meets the CAP criteria listed above

RE: [Histonet] Cryostat cleaning

2013-08-16 Thread Morken, Timothy
Behalf Of Ann Specian Sent: Friday, August 16, 2013 8:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat cleaning ANP.12087 states that the cryostat needs to be defrosted and wiped clean with a tuberculocidal. Can someone tell me what they use that meets the CAP criteria

[Histonet] Cryostat cleaning

2013-08-16 Thread Ann Specian
ANP.12087 states that the cryostat needs to be defrosted and wiped clean with a tuberculocidal. Can someone tell me what they use that meets the CAP criteria listed above. thankis, ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://li

[Histonet] Cryostat decontamination

2013-08-16 Thread anita dudley
Just curious how often everyone is taking their cryostats down, I know cap says every week if used daily. Thanks ahead for the info. Everyone have a great weekend Anita Dudley Providence Hospital Mobile Alabama __

Re: [Histonet] Cryostat recommendations

2013-08-09 Thread Dmitry Kislov
Hi, Leica 1850s are very reliable, 1860 is basically the same unit with very precise electronic specimen feed. UV lamp's lifetime is about 8k hours, and every switch on cycle adds another hour to the counter. New lamp will cost you a few hundred euro, though. Any operator can replace the lamp, no

[Histonet] Cryostat recommendations

2013-08-09 Thread Rathborne, Toni
Happy Friday everyone! I'm looking for some feedback on cryostat preferences. We are replacing one of ours now and will be evaluating a few before making a final decision. The 2 I've looked into so far are the Leica 1860 and the HM525 by Thermo Scientific. I'm interested in the UV feature that m

RE: [Histonet] Cryostat Cleaning

2013-08-06 Thread Morken, Timothy
: [Histonet] Cryostat Cleaning Can someone tell me the CAP approved chemical that can be used to clean the cryostat? thanks, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Cryostat Cleaning

2013-08-06 Thread Ann Specian
Can someone tell me the CAP approved chemical that can be used to clean the cryostat? thanks, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Cryostat Repair Service in San Diego

2013-06-17 Thread dusko trajkovic
Good Morning, Can anyone recomend a good, reliable cryostat repair service in the San diego area? thanks Dusko ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] cryostat decontamination

2013-02-07 Thread Kolman, Kimberly D.
Has anyone come up with a documented and referenced procedure for decontamination of a cryostat using 37% Formaldehyde? I pulled up old notes in the Histonet archives and saw that Tim Morken was working on this but that was a number of years ago. Thanks, Kim Kimberly D. Kolman,

RE: [Histonet] cryostat

2013-02-06 Thread Jones, Laura
..@hotmail.com] Sent: Wednesday, February 06, 2013 8:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat Hi,I wonder what is the way of removing shavings/trimmings from the cryostat in other lab?, with the wet paper? gauze?, household vacuum cleaner - yes I saw this in

RE: [Histonet] cryostat

2013-02-06 Thread Elizabeth Chlipala
-boun...@lists.utsouthwestern.edu] On Behalf Of Alan Bright [abri...@brightinstruments.com] Sent: Wednesday, February 06, 2013 9:31 AM To: IRENA SREBOTNIK KIRBIS Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] cryostat I would not vacuum the trimmings out of cryostat, if it were

Re: [Histonet] cryostat

2013-02-06 Thread Alan Bright
I would not vacuum the trimmings out of cryostat, if it were infected It would make a good job of contaminating a laboratory etc. Terrified Alan Bright Sent from my iPhone > > > > > Hi,I wonder what is the way of removing shavings/trimmings from the cryostat > in other lab?, with the wet p

[Histonet] cryostat

2013-02-06 Thread IRENA SREBOTNIK KIRBIS
Hi,I wonder what is the way of removing shavings/trimmings from the cryostat in other lab?, with the wet paper? gauze?, household vacuum cleaner - yes I saw this in one lab!?thanksIrena ___ Histonet mailing l

[Histonet] CRYOSTAT PROBLEM

2012-11-29 Thread Lakshmi Kammili
Hi all, We have a old cryostat (Microm h505 model). It was making some sudden vibrating noises for a little while and then smooths it self. There  are no temperature alterations every thing was fine but suddenly it stopped working, no display more looks like dead :(  I tried calling the service

[Histonet] Cryostat repair

2012-11-01 Thread Michael J. Lyon, Ph.D.
We have a: Reichert HistoSTAT 975CJ cryostat microtome model: 855 serial #: 0819-3410 That needs attention. Any suggestions? Thanks Michael J. Lyon, PhD Otolaryngology Research Labs SUNY Upstate Medical University 750 East Adams Street Syracuse, NY 13210 Voice: 315-46

[Histonet] Cryostat sectioning

2012-03-21 Thread Chen, Shu-Cheng
Hi, We are searching for a fee for service lab experienced in cryostat sectioning of animal and human tissues. Any recommendation is appreciated. Thanks, Shu-Cheng Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse

[Histonet] Cryostat

2012-02-29 Thread Rolly Perez
Anyone interested in a used, working Cryostat, contact me directly: Leica/Reichert-Jung CRYOCUT 1800 Rolly Perez, HT(ASCP) Histology Supervisor MedSurg Pathology Associates, Inc. Tigard, OR 503.443.2157 ___ Histonet mailing list Histonet@lists.utsou

[Histonet] Cryostat cutting?

2012-01-15 Thread Francis OBrien
Hello, We need to cut clean and consistent 50 micron sections of OCT embedded tissue on our leica cryostat (CM-1850). Does anyone on the list have any tips or tricks for doing this when compared to cutting the usual <10 micron sections that we usually deal with in our lab. -- Regards, Francis

[Histonet] Cryostat Temp settings?

2012-01-10 Thread Kasai, Miki (NIH/NCI) [E]
Hi, We are currently sectioning mostly mouse lung and liver tissue embedded in OCT. Our cryostat temp. settings are as follows: 1. OT -13°C 2. CT -17°C These temperatures were set during initial setup of the instrument by the field tech. Our cutting is ok (obviously, lung is a bit more pro

RE: [Histonet] Cryostat Maintenance and Repair - CA

2011-05-04 Thread Laurie Colbert
number is (800) 377-5395. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eric Velazquez Sent: Wednesday, May 04, 2011 2:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat M

[Histonet] Cryostat Maintenance and Repair - CA

2011-05-04 Thread Eric Velazquez
Hi, Can somebody please recommend to me a company that does cryostat maintenance and repair? I am looking for a company that is familiar with both Micron and Leica models in the Irvine, CA area. Thanks, Eric ___ Histonet mailing list Histonet@lists.utso

RE: [Histonet] cryostat repair--thanks!

2011-04-05 Thread Weems, Joyce
@lists.utsouthwestern.edu Subject: Re: [Histonet] cryostat repair--thanks! The compressor fan was loose, so when the Tech One guy fixed it, instead of making a horrible noise when it turns off, it just rattles. Oh well, time for a new motor. Which leads me to another question, is there any reason I should call Lecia

Re: [Histonet] cryostat repair--thanks!

2011-04-05 Thread Emily Sours
The compressor fan was loose, so when the Tech One guy fixed it, instead of making a horrible noise when it turns off, it just rattles. Oh well, time for a new motor. Which leads me to another question, is there any reason I should call Lecia to replace this motor? The guy from Tech One Biomedical

[Histonet] cryostat repair--thanks!

2011-04-01 Thread Emily Sours
Thanks to all who answered my email, I ended up calling Tech One Biomedical and they came right out. I recommend them to anyone who needs histology equipment repaired!! Emily ps. this is not an april fool's joke. pps. really! It has become almost a cliche to remark that nobody boasts of ignoranc

RE: [Histonet] cryostat repair

2011-03-31 Thread Bernice Frederick
: [Histonet] cryostat repair We are using Tech One Biomedical... service reps in Pghfor almost all of our routine instrument service really good and half the price of the vendors! Their number is 866-497-3033 or www.techoneweb.com Please note that my email address as of Jan 3, 2011 is bouch

RE: [Histonet] cryostat repair

2011-03-31 Thread Bouchal, Rena L
DANIEL HEWITT Sent: Thursday, March 31, 2011 2:15 PM To: Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryostat repair Emily You can try Ray Brodersen at Brodersen Instrument Co, they are in Mars and do most of our repairs. Ray does an excellent job at a good price. It sounds

RE: [Histonet] cryostat repair

2011-03-31 Thread DANIEL HEWITT
@lists.utsouthwestern.edu Subject: [Histonet] cryostat repair Our cryostat (CM3050S) is making a terrible noise when the fan stops running, yet I don't want to call Leica (the manufacturer) because they charge more money than anyone could ever afford. How would I go about finding someone who could rep

[Histonet] cryostat repair

2011-03-31 Thread Emily Sours
Our cryostat (CM3050S) is making a terrible noise when the fan stops running, yet I don't want to call Leica (the manufacturer) because they charge more money than anyone could ever afford. How would I go about finding someone who could repair a cryostat in Pittsburgh? I don't think it's the refri

RE: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED)

2011-03-30 Thread Sheila Adey
Hi Diane, We just purchased a cryostat from Sakura, we love it. Great features. > Date: Wed, 30 Mar 2011 06:38:41 -0700 > From: dch...@yahoo.com > To: diane.glad...@us.army.mil; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED) &g

Re: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED)

2011-03-30 Thread Phyllis Thaxton
From: "Gladney, Diane C Ms CIV USA MEDCOM MACH" To: "histonet@lists.utsouthwestern.edu" Sent: Wed, March 30, 2011 7:50:23 AM Subject: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED) Classification:  UNCLASSIFIED Caveats: NONE Dear Histonetters, Our 11 year old cryo

[Histonet] Cryostat Feedback Needed (UNCLASSIFIED)

2011-03-30 Thread Gladney, Diane C Ms CIV USA MEDCOM MACH
Classification: UNCLASSIFIED Caveats: NONE Dear Histonetters, Our 11 year old cryostat is beginning to experience some unusual problems. Our Medical Maintenance says that they won't put a lot of money into fixing it because of the age of the unit. Therefore, I am asking for opinions, pros, con

[Histonet] Cryostat CM 3050

2011-01-15 Thread bce_services
Hi all,  I'm looking for an old Leica CM3050 cryostat if anyone has a broken or mothballed one they want to sell for parts, Thanking you in advance Brian B A Cornett-Earley BCE Services,LLC Web: bceservicesllc.com Tel 770-329-6370 ___ Histonet mailing l

RE: [Histonet] Cryostat help

2011-01-11 Thread Setlak, Lisa
-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, January 11, 2011 10:29 AM To: histonet@lists.utsouthwestern.edu; KimberlyBlundon Subject: Re: [Histonet] Cryostat help That depends on what staining you need to use. If an IHC, just fi

Re: [Histonet] Cryostat help

2011-01-11 Thread Rene J Buesa
uire fixation and using the specific protocol. Always remember that it is a frozen section and therefore more delicate and less adhered than a paraffin section. René J. --- On Tue, 1/11/11, Blundon, Kimberly wrote: From: Blundon, Kimberly Subject: [Histonet] Cryostat help To: hist

[Histonet] Cryostat help

2011-01-11 Thread Blundon, Kimberly
Hello Histonetters, I am new to the histology world and I was hoping to get some feedback about staining after cutting sections in the cryostat. Does anyone have a protocol they use for staining after sectioning tissue in a cryostat? I have never done it before so I thought I would com

RE: [Histonet] Cryostat Question Please

2010-11-29 Thread Sherwood, Margaret
27;Donnell, Bill Sent: Wednesday, November 24, 2010 4:14 PM To: Paula Lucas; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cryostat Question Please Very consistant, easy to use. 2 drawbacks 1. We got the decontamination add-on trying to automate the CAP requirement, but now it se

RE: [Histonet] Cryostat Question Please

2010-11-24 Thread O'Donnell, Bill
arney, NE 68847 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Wednesday, November 24, 2010 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat Question Please Hello a

[Histonet] Cryostat Question Please

2010-11-24 Thread Paula Lucas
Hello all Has anyone used a Richard-Allan/Thermo-Fisher HM550 MP cryostat? If so, what is your opinion/pros and cons? Thanks so much in advance, Paula Lab Manager BP Medical Group ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://li

Re: [Histonet] cryostat question

2010-11-01 Thread Alan Bright
wireless device -Original Message- From: histo...@aol.com Sender: histonet-boun...@lists.utsouthwestern.edu Date: Fri, 29 Oct 2010 22:10:21 To: Subject: [Histonet] cryostat question Does anyone have 'hands on' experience performing Mohs using a countertop cryostat? Can the specim

[Histonet] cryostat question

2010-10-29 Thread historsd
Does anyone have 'hands on' experience performing Mohs using a countertop cryostat? Can the specimen head be manipulated to acccomodate a badly embedded block? Does the machine stay cold enough to perform Mohs? Any advice would be appreciated. My e-mail is histo...@aol.com. Thanks, Susan __

[Histonet] Cryostat correction

2010-09-23 Thread Andrew Burgeson
lol...At the end of my last post I meant to say, "I DO NOT think he knows what he is doing!" oops ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Cryostat decontamination

2010-09-03 Thread thisisann
Can someone send me a procedure on how to decontaminate a cryostat? Thank you, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] cryostat

2010-08-22 Thread histotech
: 'Jeffrey Thompson'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryostat Jeff, We have both the Leica CM1850 and the CM1850 UV. These are not top of the line models, but they work very well for us. You can find out more information on the unit at the following URL: http:/

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