[Histonet] Influenza A IHC
If anyone is still doing Influenza A IHC testing, could you share your vendor source and catalog number with me? I'm looking to find a better source than what I'm using right now. I don't do it very often (since we have a PCR test) but once in awhile it's requested, and my old antibody just doesn't seem to detect all that it should. Thanks in advance, Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Thyroglobulin TGB04+TGB05 Not Working in Canine and Feline
Hi Sandra, Being a mouse monoclonal, it may not cross-react with canines/felines; the epitope may be specific to human follicular cells only. The polyclonal Thyroglobulin from Dako/Agilent works fine on all the mammals I've tried it on so far (and a frog as well). Regards, Jan Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* On Wed, May 15, 2019 at 10:54 AM Sandra Cheasty via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello, > We had to switch sources (to Novus) for Thyroglobulin > TGB04+TGB05 and cannot get it to work in canines or felines. Data sheet > suggests 1:100, pH6 citrate antigen retrieval. We've also tried zero AR and > pH9 EDTA AR, to no avail. Any suggestions? > Thanks! > Sandy > > Sandra J. Cheasty, HT (ASCP) > Histology & Necropsy Supervisor > UW-Madison, School of Veterinary Medicine > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Chromogranin A in dogs
Hi Lisa, I just worked up an antibody from abcam; cat. # ab85554 Rabbit polyclonal 1:1200 (0.6ug/ml) -1:1600 (0.4 ug/ml) Heat retrieval in pH 6.0 solution 45 min primary Ab, 45 min secondary Ab (polymer system), 15 min chromogen (AEC+) Regards, Jan Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* On Wed, May 15, 2019 at 10:20 AM Lisa Parsons via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Our CgA has stopped working (IHC/P). Does anyone have a source for a CgA > that works in dogs? > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC Control Slide Longevity
Hi Sandy, We cut CD31, CD45, CD45RA, CD117, CD204, and MUM1 controls fresh (same morning as staining; case material as well). We've never had any problems with Synaptophysin; it's always worked very well on unstained controls that are up to a year old (stored in the fridge). Best regards, Jan Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* On Thu, Feb 28, 2019 at 12:57 PM Sandra Cheasty via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello all, > Do any of you have to cut any of you antibody controls > "fresh"? We currently cut the MUM-1 fresh, and the Synaptophysin. (We > seldom do a Syanpt., and have found the fresh cut patient tissue has been > positive while the not-so-fresh positive control slide is negative. > Any comments are - as always, are appreciated. > Cheers, > Sandy > > Sandra J. Cheasty, HT (ASCP) > Histology & Necropsy Supervisor > UW-Madison, School of Veterinary Medicine > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Not DAB IHC waste
Hello, It depends upon which 'red' chromogen you are using. There are several with different chemical compositions, so you will need to look at the Safety Data Sheets which pertain to the chemical you are using. In my lab we use 3-amino-9-ethylcarbazole (AEC) which is considered hazardous. It causes eye and skin irritation, may cause serious eye damage, and may cause cancer. We ship our waste off for proper disposal; it does not go down the water drain. Best regards, Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* On Fri, Feb 22, 2019 at 9:34 AM Histology via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi all, > > Does anyone use only red or magenta detection for IHCs instead of DAB? We > do not use DAB and are trying to figure out if our waste is hazardous or > not. Any insight would be helpful. > > Thanks, > > Mehndi Helgren > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] guinea pig IHC
Has anyone ever performed IHC on frozen sections of guinea pig tissue? I am experiencing an enormous amount of bubbling when doing the peroxidase blocking step, even though I'm only using a 0.3% concentration of H2O2. And when I say 'enormous', I mean it's like continuous champagne bubbles rising out of the tissue, even after 20 minutes in the H2O2 solution. I can't find anything in the literature that mentions guinea pigs having a higher peroxidase content in their tissues. Thanks for any help that anyone can provide. Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD204 Anti-MSR1 monoclonal, clone SRA-E5
Hi Sandy, I get mine from CosmoBio, catalog # KAL-KT022. Needs heat retrieval and I go 1 hr incubation in primary Ab at room temp. Good luck, Jan Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* On Thu, Sep 27, 2018 at 1:40 PM Sandra Cheasty via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello all, > Does anyone know where I can get CD204, Anti-MSR1 > monoclonal, clone SRA-E5? > Thanks! > Sandy > > Sandra J. Cheasty, HT (ASCP) > Histology & Necropsy Supervisor > UW-Madison, School of Veterinary Medicine > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bordetella IHC
Are there any veterinary labs that do Bordetella IHC and can accept tissue from outside of the US? Asking for a colleague. Thanks in advance. Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Adenovirus antibody?
Hello Paula, This isn't an answer to your question, because I do use the Millipore antibody (MAB805) and it works quite well on a Dako platform or done manually. It requires enzyme digestion antigen retrieval. I use it at a 1:400 dilution (45 min) with a polymer detection system (45 min). I'm not sure why it works on one platform but not another. Best of luck, Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* On Thu, Aug 3, 2017 at 8:47 PM, Tony Henwood (SCHN) via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi Paula, > > We use Adenovirus antibody from BioSB (Diagnostic Technology) > Cat Number BSB 5040 > Clone 20/11 and 2/6 > Isotype IgG1/K > Dilution1:200 with citrate retrieval on the Bond 3 > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children's Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -Original Message- > From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu] > Sent: Friday, 4 August 2017 8:50 AM > To: HistoNet > Subject: [Histonet] Adenovirus antibody? > > Hello Listers, > > I am listing to starboard on this one. We are using the Ventana Ultra > staining platform and have had a bit of trouble with our Adenovirus > stains. We have used Abcam and Millipore to no avail. > > Do you have suggestions about another vendor to try? We are a clinical > lab so we only stain human tissue. IVD is preferred but ASR and RUO are OK > too. > > Any suggestions are gratefully accepted. > > Thank you in advance. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 <#> > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this e-mail in error, > please contact the sender and delete the material from any computer. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain > confidential information. If you are not the intended recipient, please > delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and > are not necessarily the views of NSW Health or any of its entities. > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] EMA/MUC1 IHC on dogs
Does anyone perform EMA/MUC1 IHC on canine tissue? If so, what company is your vendor? You can reply to me in a private message. Thanks in advance. -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD68 and CD204 for dogs/pigs
Hi all, I do have MAC387 and it works great in dog and pig tissue. The PI is asking for additional CD markers to macrophages (CD68 and 204). I need them to work on dogs and pigs, but of course, there's not many vendors who list species cross-reactivities on their data sheets beyond the usual human and mouse. In my earlier trials years ago with CD68 (clone KP1), I didn't have success using it on dog tissue. Am looking for a different clone that will cross-react with dogs. Jan On Wed, Feb 25, 2015 at 11:08 AM, Elizabeth Chlipala l...@premierlab.com wrote: Jan MAC387 is a marker that we have used successfully in canine and porcine, not entirely specific to macs but may work for your situation. LIz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Tuesday, February 24, 2015 3:59 PM To: histonet Subject: [Histonet] CD68 and CD204 for dogs/pigs Can anyone recommend vendors for CD68 and CD204 that work on dogs and pigs (on FFPE tissue)? I don't seem to find much information on species cross-reactivity online. Thanks much in advance. -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Clinical EM Labs?
There is a listserver which is similar to Histonet for EM providers/investigators: http://www.microscopy.com/ This may provide some networking possibilities for those who are looking for EM lab information. Jan Shivers Veterinary Diagnostic Laboratory University of Minnesota On Mon, Jul 21, 2014 at 7:31 AM, Cartun, Richard richard.car...@hhchealth.org wrote: We closed our EM laboratory many years ago after our Director passed away unexpectedly and we could not find anyone to take his place. We now send all of our medical and transplant kidney biopsies to Columbia in NYC for diagnosis including EM. Other specimens (e.g., ciliary biopsies, heart biopsies for amyloid, an occasional liver biopsy, etc.) are sent to Yale. Although we run a large Anatomic Pathology operation there are very few specimens that require EM. We no longer use EM for tumors and most infectious disease cases are resolved with IHC. It's unfortunate (given our size) that we can no longer provide these services on-site; however, I'm a firm believer of Centers of Excellence and I think sending these specimens out to centers that are performing EM on a daily basis is best for the patients that we serve. Richard Cartun From: histonet-boun...@lists.utsouthwestern.edu [ histonet-boun...@lists.utsouthwestern.edu] on behalf of Paula Sicurello [ pat...@gmail.com] Sent: Friday, July 18, 2014 4:11 PM To: HistoNet; microsc...@microscopy.com Subject: [Histonet] Clinical EM Labs? Hello Netters and Listers, I was wondering how many clinical EM labs there are in the US? I get the feeling that we are becoming a small group and just wanted to see who else is out there. My lab has two services: surgical pathology and diagnostic virology. Please contact me if you are clinical EM as well. I figured, if we are a small group, we should get to know each other. Thanks and have a nice weekend. Sincerely, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Control block validation requirements
Denise, When selecting infectious agent positive controls, I also select cases which have been shown to be positive by other testing means (EM, Bacteriology, Virology, PCR). In addition, I run a cross-reactivity validation with my antibody on other infectious agent positive control slides to make sure my antibody is specific for the infectious agent and doesn't cross-react with other viruses/bacteria, etc. Results go into a table that is stored along with the initial antibody validation/optimization logsheet(s). When validating tissue antigens/tumor markers, I use the obvious normal anatomic cell/tissue control for that antigen. My anatomic pathologist advisor signs off on the final optimization for all antibodies before the IHC test can be put into public use. Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu On Tue, Jul 15, 2014 at 2:05 PM, Long, Denise denise.l...@uconn.edu wrote: Same inspectors advised our special stain and IHC controls should have at least two if not three different validation means (PCR, micro, serology, etc.) Anyone out there actually doing this? Just so you know, I've spoken with a few suppliers on control slides and they do not do this type of validation on their control material (surprise, surprise!) Thanks, Denise M. Long, MS, HTL (ASCP), QIHC University of Connecticut Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical Diagnostic Laboratory 61 N. Eagleville Road, Unit 3089 Storrs, Connecticut 06269-3089 (860) 486-0851 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Friday histology trivia
The Ki in Ki-67 is from the city of origin (Kiel, Germany), and is really pronounced KEY-67 (not K. I. 67). The 67 in Ki-67 is from the original clone in a 96-well plate. Happy Friday! -- Jan Shivers Senior Scientist University of Minnesota On Fri, Jun 27, 2014 at 1:24 PM, Morken, Timothy timothy.mor...@ucsfmedctr.org wrote: The LEI in Leic comes from Leitz; the CA, from Cambridge. Barbara, You get the award for best trivia fact of the year! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Foster Sent: Friday, June 27, 2014 10:27 AM To: Histonet Subject: [Histonet] Friday histology trivia You are so right. Microscopes equal credibility. Maybe because we can actually see an image of the specimen rather than a squiggly line... or maybe because people actually sit at microscopes rather than at an impersonal control panel. Re: AO - They were part of the American Optical/Reichert-Jung Family. In 1986, they were acquired by Cambridge Instruments, which then merged with Leitz in 1991. The LEI in Leic comes from Leitz; the CA, from Cambridge. I had the privilege of being the Technical Marketing Manager for the research microscopy group, then, for a short period, product development manager for a suite that included the AO clinical line. Best regards, Barbara Foster ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Aperio slide scanner
My department may purchase an Aperio slide scanner in the near future. My question is - how tech savvy does one need to be to operate the device? I have staffing concerns and the amount of training time involved. Thanks in advance. -- Jan Shivers IHC/Histology Section Head Pathology Teaching Program University of Minnesota shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Rabies tissue Fixed in Formalin.
*SUSCEPTIBILITY TO DISINFECTANTS*: Rabies virus is inactivated by exposure to 70% ethanol, phenol, formalin, ether, trypsin, β-propiolactone, and some other detergents(3 http://www.phac-aspc.gc.ca/lab-bio/res/psds-ftss/rab-eng.php#endnote3). (Found in the Public Health Agency of Canada article http://www.phac-aspc.gc.ca/lab-bio/res/psds-ftss/rab-eng.php) We often do IHC testing for Rabies on formalin-fixed, paraffin-embedded tissues here, with no extra precautions needed. Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu On Thu, Jun 5, 2014 at 1:12 PM, Stephenson, Sheryl sheryl.stephen...@ag.state.nj.us wrote: Hi, Does anyone have any knowledge and literature on whether or not 10%NBF inactivates rabies in animal tissue during fixation? Thanks, Sheryl. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Rabies tissue Fixed in Formalin.
Yes, Tresa mentions an important caution. Personnel need to be vaccinated against rabies if they're cutting in tissue. Jan Shivers On Thu, Jun 5, 2014 at 2:24 PM, Goins, Tresa tgo...@mt.gov wrote: Sheryl - NBF will inactivate the rabies virus but precautions should be taken. If a rabies case is suspected but infection can not be confirmed or ruled out due to the tissue being unsuitable for rabies testing by fresh tissue fluorescent antibody assay, the tissue is cut in for FFPE sections ONLY by personnel that are vaccinated against rabies. Tresa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stephenson, Sheryl Sent: Thursday, June 05, 2014 12:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Rabies tissue Fixed in Formalin. Hi, Does anyone have any knowledge and literature on whether or not 10%NBF inactivates rabies in animal tissue during fixation? Thanks, Sheryl. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC on animals
Dr. Raff, You will need to do trial runs to first determine if the antibody can detect the specific epitope in animal tissues (epitopes aren't always conserved between species), and then do your own optimization to determine the best dilution and tissue pretreatment for that particular species. They can vary by species, and don't always coincide with the recommendations on the antibody data sheet provided by the vendor. -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu On Wed, Mar 26, 2014 at 3:03 PM, Lester Raff MD lr...@uropartners.comwrote: Hi: Can IHC validated for humans be performed on animal tissues without any protocol changes? Thanks, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for PEDV antibody vendor
Hello all, Can anyone suggest a stateside vendor for Porcine Epidemic Diarrhea Virus antibody? My overseas vendor is discontinuing their veterinary products and I'm in great need of a constant and reliable supply. Thanks in advance, -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Email list
Stephanie, et al Oops, the previous email in this string was an answer on how to unsubscribe from the Histonet. I copied and pasted the link from my own information email, not realizing that it contained MY Histonet email address on the end. Please DISREGARD the link (or delete the link ending after '/histonet/' where my email address is listed and THEN use it to unsubscribe). Sorry - I think the polar vortex has partially frozen my brain this winter! Jan Shivers UMN Vet Diag Lab St. Paul, MN On Wed, Feb 26, 2014 at 11:42 AM, Jan Shivers shive...@umn.edu wrote: From the Histonet guidelines when one subscribes: If you ever want to unsubscribe or change your options (eg, switch to or from digest mode, change your password, etc.), visit your subscription page at: http://lists.utsouthwestern.edu/mailman/options/histonet/shive003%40umn.edu -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu On Tue, Feb 25, 2014 at 5:27 PM, Stephanie Moore numberf...@gmail.comwrote: Good Afternoon, I would like to know how I can be taken off the email list for Histonet. Thank you, *Stephanie Huff, B.S., HTL(ASCP)CM* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Email list
From the Histonet guidelines when one subscribes: If you ever want to unsubscribe or change your options (eg, switch to or from digest mode, change your password, etc.), visit your subscription page at: http://lists.utsouthwestern.edu/mailman/options/histonet/shive003%40umn.edu -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu On Tue, Feb 25, 2014 at 5:27 PM, Stephanie Moore numberf...@gmail.comwrote: Good Afternoon, I would like to know how I can be taken off the email list for Histonet. Thank you, *Stephanie Huff, B.S., HTL(ASCP)CM* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Which] Buffer for IHC
To calibrate a pH meter, we use purchased pH buffers at pH 4.0, 7.0, and 10.0. To perform the IHC method, we use TBS (pH 7.6) with Tween 20 as our rinse buffer. Jan Shivers On Thu, Jan 23, 2014 at 2:52 AM, Edwards, Richard r...@leicester.ac.ukwrote: And what is the buffer of choice, PBS, TBS, with or without protein/detergents or whatever, thanks. Richard Edwards Leicester University U.K. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: 22 January 2014 22:08 To: Laurie Colbert Cc: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: Re: [Histonet] Buffer pH for IHC pH meter; Corning. On Wed, Jan 22, 2014 at 3:21 PM, Laurie Colbert lcolb...@pathmdlabs.com wrote: What does everyone use to test the pH of their buffers for IHC? Are pH strips adequate or do you have to have an actual pH meter? If you use a meter, any suggestions on a make and model? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC-start-up
I work in animal tissue only. I also agree that an open autostainer system works best, since you may have to run multiple dilutions and incubation times for the same antibody because of the different species' reactivities. You may also need multiple types of reagents to cover detection in different species, so you'll need a system which will allow you to use multiple vendor products. An aside... The CD4 and CD8 Abs that I've done on various animals are usually only done on frozen sections, so I tend to perform that IHC manually, in order to be able to rinse slides more gently than an autostainer does (and do overnight incubation with primary Ab, if necessary). Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu On Thu, Jan 23, 2014 at 12:12 PM, James Watson jwat...@gnf.org wrote: Erin, We run the Ventana Discovery XT at our lab and average 15,000 antibodies stained a year using 5 stainers on animal tissue. If you are working on volume I would also look at the Leica Bond system. Leica and Ventana both have released or are releasing more flexible systems as far as programing staining protocols. With the Ventanas you are tied into their chromogen labeling systems and LCS, but most other reagents can be substituted. Other systems are more open, but do not offer on line deparaffinization or HEIR. These would be great if not working with large volume. The Ventanas have worked great for us, but like all stainers they do have their little idiosyncrasies. Look at what fits your needs and look closely at the cost of running a system vs. efficiency. Jamie James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Tel858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Will Chappell Sent: Thursday, January 23, 2014 9:45 AM To: Erin Sarricks Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC-start-up Doing ihc on animal tissue is very tricky, if for no other reason than you are trying to standardize an antibody across multiple species. You will need to use every trick in the book and invent some of your own to get reproducible results. I would steer clear of any platforms that do not let you customize everything. I would suggest an OLD Dako or biocare's IntelliPath. Both have their quirks but they have the openness you require. William Chappell Sent from my iPhone On Jan 23, 2014, at 11:49 AM, Erin Sarricks esarri...@gmail.com wrote: Hi all- Our histopathology lab is looking to set up an IHC component to service the request of our clients. All work is on animal tissue. We will probably run about 1,000 IHC slides the first year and hope to increase the workload each year. Some stains we would run would include CD4, CD8, Ki-67 and TUNEL. We looked at the Ventana Discovery XT and appeared to be a good fit for our needs. Does anyone have any other advice on other machines we should look into? Any information or advice would be greatly appreciated. Thank you! Regards, Erin Sarricks, HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Buffer pH for IHC
pH meter; Corning. On Wed, Jan 22, 2014 at 3:21 PM, Laurie Colbert lcolb...@pathmdlabs.comwrote: What does everyone use to test the pH of their buffers for IHC? Are pH strips adequate or do you have to have an actual pH meter? If you use a meter, any suggestions on a make and model? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: [IHCRG] macrophage eq markers?
I've had it to work in cat, cow, dog, fox, horse, otter, pig, and raccoon in my lab. According to vendor - it's supposed to also work in guinea pig, monkey, rabbit, and rat, but I have not tried it in those animals Needs either enzyme digestion or heat antigen retrieval. Best regards, Jan Shivers On Mon, Dec 2, 2013 at 3:37 PM, Mehmet Fatih BOZKURT fbozk...@gmail.comwrote: In which animals works also this antibody ? Thanks On Mon, Dec 2, 2013 at 11:20 PM, Jan Shivers shive...@umn.edu wrote: Dako's MAC 387 (cat. #: M0747) On Mon, Dec 2, 2013 at 2:09 PM, Jackie Ferracone ferra...@vet.upenn.eduwrote: Hi All, Looking for good macrophage markers for equine tissue, FFPE IHC .? Thanks Jackie Ferracone, B.Sc. Research Specialist Dept. of Pathobiology University of Pennsylvania School of Veterinary Medicine 382 West Street Road Kennett Square, PA 19348-1692 610-925-6391 610-350-8860 (cell) 610-925-6810 (fax) -- -- You received this message because you are subscribed to the Google Groups ihcrg group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ih...@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscr...@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: hi...@nsh.org or call 443.535.4060. --- You received this message because you are subscribed to the Google Groups ihcrg group. To unsubscribe from this group and stop receiving emails from it, send an email to ihcrg+unsubscr...@googlegroups.com. For more options, visit https://groups.google.com/groups/opt_out. -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu -- -- You received this message because you are subscribed to the Google Groups ihcrg group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ih...@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscr...@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: histo@nsh.orgor call 443.535.4060. --- You received this message because you are subscribed to the Google Groups ihcrg group. To unsubscribe from this group and stop receiving emails from it, send an email to ihcrg+unsubscr...@googlegroups.com. For more options, visit https://groups.google.com/groups/opt_out. -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Shipping to Countries Outside of the US
According to our Receiving department, each country/institution has its own permit requirements for receiving samples from outside of their own country. It is up to the shipper to inquire of the receiver as to what permits are needed to ship to that country (Germany, in your case). My source also said that FedEx would also have good information on this, since they ship nearly everywhere around the globe. Jan Shivers On Wed, Oct 2, 2013 at 11:10 AM, Sheila Miller smmil...@comhs.org wrote: Hello All, A question was posed to me by our Cancer Resource Department... I am wondering if you/lab has had experience with shipping tissue specimens outside the country. We have a study where we may be doing this and they are asking if we have to have a special permit to ship outside our country. I believe it is going to Germany. Thank you, Sheila This message and attachment(s), if any, is intended for the sole use of the individual and/or entity of which it is addressed, and may contain information that is privileged,confidential and prohibited from disclosure under applicable law. If you are not the addressee, or authorized to receive this on behalf of the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone this message or any part thereof. If you have received this in error, please immediately advise the sender by e-mail and delete this information and all attachments from your computer and network. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] edge effect on IHC
Also make sure that the tissue never dries out on the trimming table before it even gets to formalin. That can also be a source of edge effect. Jan Shivers UMN On Wed, Sep 25, 2013 at 7:37 AM, Tim Higgins thiggin...@msn.com wrote: Hey Mary, I would suggest observing your stainer to ensure the proper drop zones are being performed and not just dropping closest to the label. I used Dako for years and loved them but you do have to make sure it dispenses properly from time to time. They use drop zones unlike Ventana which uses a vortex type of mixing. Good luck! Thanks, Timothy N. Higgins, HT (ASCP), QIHC Date: Mon, 23 Sep 2013 14:00:57 -0700 (PDT) From: Mary Benoit mbp...@yahoo.com Subject: [Histonet] edge effect on IHC To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 1379970057.43816.yahoomail...@web142505.mail.bf1.yahoo.com Content-Type: text/plain; charset=us-ascii I have been aware of some edge effect on an occasional slide, but lately we noticed the effect is showing up in the area of the slide that is farthest from the labels. We use DAKO Link Autostainer and their pretreatment baths for everything. One case in particular we stained a large melanoma met to colon that was well fixed and processed that stained only the edge, however when we sent block to Neogenomics reference lab, their slide stained evenly over the entire tumor area. We both used a melanoma cocktail. Not sure what is going on. Any suggestions? Thank you Mary F Benoit, MT(ASCP) The Pathology Laboratory 830 Bayou Pines Drive Lake Charles, LA 70601 mbp...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Borrelia burgdorferi IHC
Meridian Life Science has monoclonal and polyclonal antibodies to B. burgdorferi. None are ready-to-use, but two are conjugated (either to HRP or Biotin). Jan Shivers UMN On Wed, Aug 14, 2013 at 12:12 PM, Delray Beach Pathology Kari Simeone ksime...@leavittmgt.com wrote: Can anyone recommend a vendor where I can purchase ready to use Borrelia burgdorferi antibody for IHC? Thanks in advance. Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor ADCS Clinics, LLC Advanced Dermatology and Cosmetic Surgery Ameriderm 561.819.0857 ext 100 561.819.6517 fax ksime...@leavittmgt.com www.advancedderm.com http://www.advancedderm.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Plastic/Plexiglass Incubation chamber for IHC slides
When doing manuals, I use the Newcomer Supply humidity chamber that holds 20 slides, but when I run out of space, I always have my trusty metal cake pan with lid (and 1 ml disposable pipets for slide racks) from the 'old days'. Jan Shivers UMN Vet Diag Lab St. Paul, MN On Mon, Jul 8, 2013 at 1:10 PM, davidclar...@comcast.net wrote: Hi Histonetters, I hope you all had a wonderful Independence Day! Do you guys remember in the olden days before automation when we did IHC by hand? I'm looking for an incubation chamber that allows us to incubate our slides overnight. The one I have right now is about 20x6x6 with a lid and allows for about 4 flat racks of slides to be placed inside. Any information would be appreciated either if you have one or know of a company that can make one. Cheers! David Clark Oregon Health Science University Portland, Oregon ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Highlands J Virus
(Asking for a colleague...) Is there any lab out there currently doing IHC for Highlands J Virus? You can message me privately. Thanks in advance, Jan Shivers Senior Scientist Univ. of Minnesota Veterinary Diagnostic Lab shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] antigen retrieval question
Lynn, I use the Decloaker and also stain for BVD, so will get in contact with you later this afternoon. Jan Shivers University of Minnesota Veterinary Diagnostic Lab St. Paul, MN 55108 On Thu, May 30, 2013 at 9:54 AM, Burton, Lynn lynn.bur...@illinois.govwrote: If anyone out there is using a decloaker for antigen retrieval, when doing ihc staining, would you please contact me on my e-mail address? I have been struggling with a BVD test for months. It is not working consistently and I want to explore using this method along with our Benchmark XT stainer from Ventana. Thank you, Lynn Burton Galesburg Animal Disease Lab Galesburg, Il lynn.bur...@illinois.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] S100
Margaret, What S100 are you using? We use Dako's polyclonal, no antigen retrieval needed, with no problems. Jan Shivers UMN VDL On Wed, May 8, 2013 at 2:25 PM, Perry, Margaret margaret.pe...@sdstate.eduwrote: Our S100 is not working very well. I think it's too old. Can someone recommend an S100 antibody that works well in dog? It would be really nice to find a good monoclonal instead of the polyclonals. We are looking for schwannomas, astrogliomas, malignant melanomas and their metastases. Margaret Perry HT(ASCP) Veterinary Biomedical Sciences Department North Campus Drive Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HIER pressure cookers....
Third that. Jan S On Tue, Apr 30, 2013 at 8:58 AM, Blazek, Linda lbla...@digestivespecialists.com wrote: I second that! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, April 30, 2013 9:54 AM To: k. leigh adams; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HIER pressure cookers Biocare Decloaker here !! Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of k. leigh adams Sent: Tuesday, April 30, 2013 9:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HIER pressure cookers Any input as to preferred instruments would be greatly appreciated... Leigh ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program UMN Veterinary Diagnostic Laboratory University of Minnesota College of Veterinary Medicine 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] SMC Immuno and Elastin combo?
Patti, You're using a mouse monoclonal antibody on mouse tissue, so be aware that the secondary link antibody will bind to any mouse IgG it finds in the tissue (normal plasma cells, IgG in normal sera present inside blood vessels, or as infiltrating interstitial normal sera, etc.), not just to the primary anti-Actin antibody. You may have to use a mouse-on-mouse kit for the Actin staining portion, if you see too much nonspecific background in your tissue. Jan Shivers UMN Vet Diag Lab On Wed, Mar 13, 2013 at 10:58 AM, Patricia Bourassa pboura...@karospharma.com wrote: Hello, I've read some papers which reference the combination of smooth muscle cell actin immunostaining with a Verhoeff's elastin counterstain. Has anyone ever done this and if so, do you have protocol you'd be willing to share? I'm staining mouse and rat lung and have a SMC protocol working that uses an Abcam antibody and NovaRed chromagen (peroxidase). I've also done EVG staining so I'm familiar with the general process. The combination is what I'm not sure of - I gave it a try for the heck of it and wiped out the immunostain. We've just started looking at this, so nothing is set in stone and I'd be grateful for any advice/ procedural help. Thanks in advance! Patti Bourassa Karos Pharmaceuticals -- *Patti Bourassa Senior Scientist Karos Pharmaceuticals (203) 535-0540, ext 207* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] job opening in St. Paul, MN
The University of Minnesota Veterinary Diagnostic Lab has a full-time job opening for a histotechnician/histotechnologist. Briefly, the job entails processing, embedding, microtomy, and routine/special stains of fixed and frozen animal tissues; operating, maintaining, and troubleshooting histologic equipment; performing manual and IHC techniques; participating in research projects with principle investigators; assisting in maintaining quality control documentation records; ordering, organizing, and maintaining histology supplies; and other duties as needed. The UMN VDL handles tissues of all species, except humans. We receive submissions from all over the world - food animals, exotic zoo animals, companion animals, laboratory animals, wildlife. For more information, please contact me directly. Jan Shivers UMN VDL Histology/IHC Section Head St. Paul, MN shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ki67 antibody to stain Mouse tissue
We have had success with Lab Vision's rabbit polyclonal on mouse tissue (catalog # RB-9043), utilizing HIER for antigen retrieval. Jan Shivers U of MN Vet Diag Lab On Mon, Aug 6, 2012 at 1:48 PM, Eva Permaul e...@georgetown.edu wrote: We are staining straight mouse tissue. On Mon, Aug 6, 2012 at 2:46 PM, Sarah Dysart sdys...@mirnarx.com wrote: Are you staining human xenografts in mice or straight out mouse tissue? I get all mine from abcam. I am currently staining xenografts so I am using a human specific one, but I think it might be good for mouse too? Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eva Permaul Sent: Monday, August 06, 2012 1:44 PM To: histonet Subject: [Histonet] Ki67 antibody to stain Mouse tissue Hello, I am looking for a Ki67 to stain mouse tissues. We have been using Dako M2749 but just found out it has been discontinued. Could anyone suggest a good alternative? Thank you all for any help, Eva Permaul Georgetown University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Olig2
I'm asking for a colleague - Does anyone know a lab that does IHC staining for Olig2? Thanks in advance, Jan Shivers Univ. of Minnesota Veterinary Diagnostic Lab St. Paul, MN shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Neutrophil Antibodies
Hi John, In the past, I used Neutrophil Elastase from Dako on monkey FFPE tissue; no pretreatment needed; dilution with lot used = 1:50. Worked great. Jan Shivers UMN Vet Diag Lab On Sat, May 5, 2012 at 9:59 AM, John Shelley jshel...@sanfordburnham.orgwrote: Hi All in Histoland, Problem solving never ends! I am working up two antibodies for neutrophils which are NIMP-R14 from Abcam which it is a rat monoclonal and also Anti-Neutrophil Elastase also from Abcam but this is a Rb polyclonal. I have tried different conditions without any luck and was wondering if anyone has worked with these antibodies either from Abcam or another supplier and might have working conditions that have given results in the past. We are trying this on FFPE sections which the antibodies say they work on but I am thinking that I may try frozens. I was trying to avoid this however because I am using these antibodies on mouse WAT. Any help would be appreciated. Thanks!!! Happy Monday morning!!! Kind Regards! John J Shelley ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Looking for protzoal antibodies
I get my Neospora caninum from VMRD; it's a goat polyclonal, but you can dilute it way out so there's no background (currently using 1:24,000). I may have a Toxo positive block for you. Jan Shivers IHC/Histo/EM Section Head Veterinary Diagnostic Lab UMN College of Veterinary Medicine St. Paul, MN On Wed, Apr 11, 2012 at 3:10 PM, Cynthia Hutchinson tily...@auburn.eduwrote: Hello All, Does anyone know of a source for clean Neospora caninum and/or Sarcocystis antibodies? Also, does anyone have any positive control tissue for toxo, neo, and/or sarco? Thank you, Cindy Cynthia Tily Hutchinson Rsch Asst IV Pathobiology Coll of Vet Med Auburn University Ph: 334-844-7020 Fax: 334-844-2652 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] EM tissue processors Q's
We use the Leica TP for our samples and endorse it. Jan Shivers IHC/Histo/EM Section Head Veterinary Diagnostic Lab UMN College of Veterinary Medicine St. Paul, MN On Wed, Apr 4, 2012 at 2:26 PM, Morken, Timothy timothy.mor...@ucsfmedctr.org wrote: Hi, we're looking around at different automated TEM tissue processors. We have an RMC 5160 but it has had a lot of problems so we are considering something else. I've looked online at Leica TP and Lynx II for routine processing. We may get to MW processing later, but the price is higher than authorized right now. Any suggestions or comments? Thanks for any info! Tim Morken Supervisor, Electron Microscopy Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94115 (415) 353-2673 (ph) (415) 514-3403 (fax) tim.mor...@ucsfmedctr.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] turkey reovirus
Does anyone know of an antibody for Reovirus that can be used successfully with FFPE tissue? I have not been able to find one online yet that can be used with IHC application. Thanks in advance, Jan Shivers IHC/Histo/EM Vet. Diag. Lab Univ. of Minnesota ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Farewell
Sally, I will miss our on- and off- topic conversations! It's been invaluable bantering back and forth with you regarding IHC in veterinary medicine, with all of its 'adventures in staining'. You must promise to enjoy every second of your retirement - do it for 'the team'. And keep posting your humorous quips on Histonet. Jan Shivers On Fri, Mar 30, 2012 at 8:41 AM, Rene J Buesa rjbu...@yahoo.com wrote: Sarah: It has been always a pleasure communicating with you as we have done so many times in the past. You are a real specialist in the difficult field of veterinary histology and you should remember that when in retirement. Try to pass on your knowledge. Being retired does not mean to go into mental hibernation. Participate in HistoNet even more than before. Now you are the owner of your time. My sincere congratulations and enjoy your retirement as I have been doing for the last 10 years. René J. --- On Fri, 3/30/12, Breeden, Sara sbree...@nmda.nmsu.edu wrote: From: Breeden, Sara sbree...@nmda.nmsu.edu Subject: [Histonet] Farewell To: histonet@lists.utsouthwestern.edu Date: Friday, March 30, 2012, 9:16 AM Hard as it is to believe - and I probably won't until I've been away for a week or more - this is my LAST day to work. Retirement? Who knew it would come around so fast? I remember having only fifteen years left to work and that was just yesterday! Histology has been 'bery, 'bery good to me and although I fell into it by accident, it has been a fascinating, involving, liberating experience. I'll be lurking on Histonet but under the alias of nmhisto and I'll probably do some p.r.n. work but I'm going home and throwing away my alarm clock and resetting the coffee maker for 6:00 a.m. instead of 3:45 a.m. Thank you all for your advice, guidance, humor, relative insanity and wisdom. Enjoy what you do and try to recruit at least one person into this field before it's your time to pick out the color of the tennis balls on your walker. Hail and Farewell and best of everything to every one of you. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Reference lab doing West Nile Virus and HHV6 IHC on human FFPE specimens
Aww, too bad. We do WNV testing on FFPE, but not on human tissue. BioReliance sells a good antibody (clone 7H2), in case anyone else out there wants to know a vendor for the antibody. Enzyme digestion works OK for antigen retrieval, but HIER works much better at exposing epitopes. Jan Shivers UMN Vet Diag Lab St. Paul, MN On Fri, Mar 30, 2012 at 10:58 AM, Sebree Linda A lseb...@uwhealth.orgwrote: Yup, that's what I'm looking for Histonetters. Any info. is appreciated. Linda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Reference lab doing West Nile Virus and HHV6 IHC on human FFPE specimens
Clarification to my previous post - we can't *accept* human tissue here. I'm sure the antibody will work on any species, however. The antibody is specific to the virus itself, not the tissue species it's residing in. Jan Shivers On Fri, Mar 30, 2012 at 11:03 AM, Jan Shivers shive...@umn.edu wrote: Aww, too bad. We do WNV testing on FFPE, but not on human tissue. BioReliance sells a good antibody (clone 7H2), in case anyone else out there wants to know a vendor for the antibody. Enzyme digestion works OK for antigen retrieval, but HIER works much better at exposing epitopes. Jan Shivers UMN Vet Diag Lab St. Paul, MN On Fri, Mar 30, 2012 at 10:58 AM, Sebree Linda A lseb...@uwhealth.orgwrote: Yup, that's what I'm looking for Histonetters. Any info. is appreciated. Linda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] blocking endogenous activities
You need to do it sometime before the HRP-labeled conjugate is put on. If you put it on after the HRP conjugate, you'll block the HRP substrate and won't see where the primary antibody is binding. I usually do mine before antigen retrieval, but have done it after the primary antibody step on frozen sections and cell smears. Jan Shivers Senior Scientist IHC/Histology/EM Veterinary Diagnostic Lab University of Minnesota St. Paul, MN On Thu, Mar 1, 2012 at 5:55 PM, Bea DeBrosse-Serra bdebrosse-se...@isisph.com wrote: The majority of the time we do it after retrieval. I believe you can do the hydrogen peroxide block at any point, as long as you do it before the DAB. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2588 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tuyen Nguyen Sent: Thursday, March 01, 2012 3:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] blocking endogenous activities In my lab, in IHC protocols for most antibodies, hydrogen peroxide is applied before antigen heat retrieval. However, I wonder there is any different result in IHC stain if hydrogen peroxide for blocking endogenous activities is applied after antigen heat retrieval by using steamer or pressure cooker? Thank you, Mai Nguyen Truong Research Associate II Los Angeles, California ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cytokeratin Lu5
Hi Margaret, We have used CK-Lu5 in the past with great results. It stains dog, cat, pig, cow, horse, goat, deer, bird, frog, hedgehog, snake, guinea pig, rat, and bats so far. Use Proteinase digestion for antigen retrieval. We did notice that in recent times the staining intensity diminished with CK-Lu5 on some runs and we had to keep dropping the dilution down to more concentrated levels to achieve the same staining intensity as in the past. Dako's CK-MNF116 is also a great broad spectrum (also stains pretty much the same animals strongly). However, CK-Lu5 stains more hepatocytes than CK-MNF116 does, in my experience. Jan Shivers Section Head IHC/Histo/EM Veterinary Diagnostic Lab Univ. of Minnesota St. Paul, MN shive...@umn.edu On Thu, Feb 23, 2012 at 3:55 PM, Perry, Margaret margaret.pe...@sdstate.edu wrote: Has anyone used this antibody on dog? If so which company did you use? Would you be willing to share your protocol? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Fwd: [Histonet] Humidity levels and IHC staining
I've had 3 Dakos and have had the drying phenomenon in all of them to some extent. We put wet paper towels in the stain sink, as others have mentioned. Plus, we found that most of the problem came from having an air gap between the hood and the unit, which wasn't closed entirely by the gasket. (The circulating room air got through the gap and dried out the slides in that front row area.) We use a long piece of styrofoam padding, cut it to length, and insert that between the bottom of the closed hood and the metal base at the beginning of each staining run, and it blocks all unwanted airflow into the slide chamber. Jan Shivers UMN -- Forwarded message -- From: Cynthia Pyse cp...@x-celllab.com Date: Fri, Feb 17, 2012 at 1:39 PM Subject: RE: [Histonet] Humidity levels and IHC staining To: Morken, Timothy timothy.mor...@ucsfmedctr.org, Rene J Buesa rjbu...@yahoo.com, histonet@lists.utsouthwestern.edu, Jason McGough jmcgo...@clinlab.com NY in winter has low humidity, I still have no problems with my Dako stainer. Make sure the lid is closed. I had a tech who consistently left the lid open, there was some drying on the first row of slides. Closed the lid the problem stopped. Are you using Dako buffer? The surfactant in the buffer should prevent drying. You might want to run it by the Dako tech service. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, February 17, 2012 2:27 PM To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Jason McGough Subject: RE: [Histonet] Humidity levels and IHC staining Renee, it can depend on where you are: Florida? 70, 80% humidity, no drying out. South Dakota, in winter? 10 percent humidity and you get drying problems. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, February 17, 2012 11:20 AM To: histonet@lists.utsouthwestern.edu; Jason McGough Subject: Re: [Histonet] Humidity levels and IHC staining A good auto stainer (like DAKO) with adequate amounts of dispensed reagents during the correct periods of time should not experiment any drying out on the slides. Adequate humidity is required to be controlled during manual IHC, especially if done over a heated support. If because of any reason (including not leveled slides) you experiment drying out, the best way would be to have an open flat dish containing water but, again, that was never a problem for me using the DAKO auto stainer. Which auto stainer are you using? René J. --- On Fri, 2/17/12, Jason McGough jmcgo...@clinlab.com wrote: From: Jason McGough jmcgo...@clinlab.com Subject: [Histonet] Humidity levels and IHC staining To: histonet@lists.utsouthwestern.edu Date: Friday, February 17, 2012, 2:09 PM We are wondering what other labs are doing to control the humidity while IHC stains are being performed. We currently place wet towels and a small weigh boat with water in our Autostainer to help prevent our slides from drying out but that seems to not be enough, they still tend to dry out and produce background staining. What should the humidity level be at? Any help would be appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Music in the Laboratory
Our policy is that if a radio station is to be played in the lab, the choice has to be a unanimous one of everyone working in the lab, it cannot be talk radio (prone to political leanings), it has to play appropriate music for work setting, and it has to be turned down low enough to almost not be audible. Personal iPods with only ONE earbud used is preferable to a room radio station. Jan Shivers On Wed, Jan 18, 2012 at 8:52 AM, Rathborne, Toni trathbo...@somerset-healthcare.com wrote: Regarding the offensive language, many institutions have policies that address the use of inappropriate language. You may find it in something like a Workplace Harassment/Violence policy. We too do not permit ear buds or headphones for the same reason. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of CHRISTIE GOWAN Sent: Wednesday, January 18, 2012 9:21 AM To: tracz...@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Music in the Laboratory Hi All, Our lab is governed by hospital regulations that no headphones or earbuds be worn at anytime. This is considered a safety issue. We do have policies governing codes for behavior such as dress, hygiene, innappropriate computer use such as streaming music or social media but music is something they allow idividual departments to dictate. Our lab has decided to allow desk top radios to be played. I have a few techs that like to listen to talk radio or music so they each have their individual radios set so low that only they can hear it. I think if everyone had their own radio it would be insane but a couple is not too bad. If it were to become distracting or a nusance, I would ban them completely. We are not in a patient traffic area but we do get a lot of outside visitors walking thru such as Resident's interviewing or clinicians. One must always consider patient care first and foremost and if music is offensive to you because of language then it probably is offensive to others as well. I think you should never have to endure music that is offensive in anyway. Jazz makes me crazy. My question to you would be, do you have a policy that stating that music is allowed? Best of luck to you. Christie From: tracz...@aol.com Date: Tue, 17 Jan 2012 21:37:04 -0500 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Music in the Laboratory Greetings. I would like to know what other histology laboratories allow for music players while working. Do you have formal policies about music content or volume? Do you allow lab space doors to remain closed to muffle the volume of what is being played? Are headsets allowed? I am a terrible judge of this because I personally prefer to work in a quiet environment. I am trying to be open minded, as long as the work gets done. However, one of the techs had a song playing today that I believe was inappropriate for general listening in the lab. Am I just out of touch? Is that dang F word just something I'm going to have to learn to accept? Do you have a written policy? When/how/why was it implemented? I should mention that it's a small private lab, with minimal patient traffic. We do see our share of FedEx, UPS, sales service reps. Your ideas on this is very much appreciated. Dorothy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Electron Microscopy Proficiency testing
We participate in the External Quality Assessment Scheme in EM Virus Diagnostics (EQA-EMV) administered through the Robert Koch Institut of Berlin, Germany. It tests proficiency only on infectious disease identification. Jan Shivers Section Head - EM University of Minnesota Veterinary Diagnostic Laboratory St. Paul, MN, USA On Mon, Jan 9, 2012 at 9:36 AM, Genest, Sharon SktnHR sharon.gen...@saskatoonhealthregion.ca wrote: Does anyone subscribe to a program for proficiency testing for Electron Microscopy? Sharon Genest Anatomic Pathology Process Improvement Saskatoon Health Region 306-655-8242 sharon.gen...@saskatoonhealthregion.ca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] mouse xGFAP on rabbit
Good Morning, Does anyone know of a *mouse* xGFAP that will work on rabbit tissue? Thanks in advance, -- Jan Shivers Senior Scientist IHC/Histology/EM Section Head Pathology Teaching Program UMN Veterinary Diagnostic Laboratory University of Minnesota College of Veterinary Medicine 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Circo antibody
Margaret, You can purchase monoclonal PCV2 antibody through RTI (Rural Technologies, Inc.). Jan Shivers Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: Perry, Margaret margaret.pe...@sdstate.edu To: ih...@googlegroups.com; histonet@lists.utsouthwestern.edu Sent: Friday, November 04, 2011 2:58 PM Subject: [Histonet] Circo antibody We are running out of our circo antibody from Iowa and did not find it listed in their bank. Are there any recommendations for a different source? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] How to save dried antibody?
I personally would not use it; you don't know how much loss of immunologic reactivity has occurred, and that might affect your project data. Buy a fresh supply, if possible. Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: sarah Tabatabaei sarah_t...@yahoo.com To: histonet@lists.utsouthwestern.edu Sent: Friday, October 28, 2011 10:27 AM Subject: [Histonet] How to save dried antibody? Dear All, We have purchased a sheep anti-CGRP antibody for our fluorescent IHC study on human tissue and kept it in -20 for a while. It came in liquid but now it's all stiff and looks like it's being crystallized. I cannot pipet any of it. It has hardened and looks like a piece of glass at bottom of its container. I tried to re-suspend it with the same amount of glycerol, but the antibody doesn't seem to be mixing with it. Does anyone know how to save this antibody? How can I bring it back to its liquid state? Thank you for your time Sarah Sadat, DDS MSc Student, Dental Sciences McGill University Montreal QC. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Abs for detecting immune cells in mouse FFPE sections
CD3 - rabbit polyclonal Ab from ThermoScientific (cat. # RB-360) works on mouse FFPE tissue. Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: Yu, Jian y...@upmc.edu To: histonet@lists.utsouthwestern.edu Sent: Wednesday, October 26, 2011 2:44 PM Subject: [Histonet] Abs for detecting immune cells in mouse FFPE sections Dear all, It will be greatly appreciated if you can share experience/information on the Abs against immune cells (vendor and cat#) that work for IHC or IF using mouse paraffin sections. Info on alternative Abs for these populations can be helpful too. I have a small research lab with limited budget for buying new Abs. Gr-1 (granulocytes) B220 (B cells) CD3 (T cells) Thank you! Jian Yu, Ph.D. University of Pittsburgh ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] new antibody lot
Ours are validated and optimized anew, just like if we were working up an entirely new antibody. Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: anita dudley azdud...@hotmail.com To: histonet@lists.utsouthwestern.edu Sent: Thursday, September 22, 2011 9:47 AM Subject: [Histonet] new antibody lot how are others handling new lot numbers of antibodies when you receive them in the lab? thanks for any input. have a great day!!! anita dudley providence hospital mobile alabama ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Where to start? Neospora and ToxoplasmaImmunohistochemistry
Hi Leah, I've done both of these for quite a number of years. You can write me privately with more questions or requests, if you like. Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: leah.simm...@industry.nsw.gov.au To: histonet@lists.utsouthwestern.edu Sent: Monday, September 12, 2011 7:43 PM Subject: [Histonet] Where to start? Neospora and ToxoplasmaImmunohistochemistry Hi Everyone I was hoping someone might be able to give me some advice on introducing Immunohistochemistry into our Laboratory. We would like to start initially with Neospora in Cattle and Toxoplasma in Sheep. Is anyone performing these and willing to help me out? Thank you Leah Simmons Industry and Investment NSW Elizabeth Macarthur Agricultural Institute State Veterinary Diagnostic Laboratory Histology Technical Officer 02 4640 6422 This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of their organisation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] TEM using difficult tissue
Daphne, We do EM work on formalin-fixed, paraffin-embedded tissue occasionally. I will send you a brief protocol under separate cover. Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: Daphne Gill dag...@upei.ca To: Histonet@lists.utsouthwestern.edu Sent: Tuesday, September 13, 2011 11:17 AM Subject: [Histonet] TEM using difficult tissue Hi I'd like to try some TEM work on rat brain hippocampus. However, the tissue is less than ideal. Does anyone have any information/insight on using old (harvested ~2 years ago) tissue that has been subjected to 20 minutes in sodium sulfide (0.4%), fixed using 30% sucrose in 10% BNF for 48 hours, and then frozen? Thanks very much for any help you can give me. Daphne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] I'm so sad for AFIP to close
The (Veterinary Pathology) Wednesday Slide Conferences are now organized under the Joint Pathology Center, so at least that service of providing histologic pathology study slides is still available. http://wsc.jpc.capmed.mil/dodvpr/wsc.php Jan Shivers UMN VDL - Original Message - From: Kim Tournear kim.tourn...@yahoo.com To: histonet@lists.utsouthwestern.edu Sent: Friday, September 09, 2011 1:39 PM Subject: RE: [Histonet] I'm so sad for AFIP to close Actually, I heard the program moved to Ft. Sam Houston in San Antonio, TX. On Fri Sep 9th, 2011 8:07 AM PDT Edwards, Richard E. wrote: Well unfortunately one can't have public/governmental spending cut backs without losing something important along the way. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sdys...@mirnarx.com Sent: 09 September 2011 16:03 To: jwe...@sjha.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] I'm so sad for AFIP to close Wow...that is sad news. Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, September 09, 2011 9:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] I'm so sad for AFIP to close I never really thought it would happen but it has... http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pat hology-Community.html Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Bovine corona virus antibody
Mouse anti-BCV from Rural Technologies, Inc (RTI); clone BC 6-4. I use my current lot at around 1:5000 with HIER (Decloaking Chamber). If you use another method of antigen retrieval, drop the dilution down quite a bit. Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: Sverlow, Karen ksver...@cahfs.ucdavis.edu To: histonet@lists.utsouthwestern.edu Sent: Friday, September 09, 2011 2:45 PM Subject: [Histonet] Bovine corona virus antibody I am looking for antibody recommendations for our bovine corona virus IHC. The clone we used previously is no longer available. Thanks, Karen Sverlow, HTL (ASCP)CM Histology Supervisor California Animal Health and Food Safety Laboratory System Thurman Building, West Health Sciences Drive Davis, CA 95616 530-752-8753 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] slide box freezer racks
Hello Ed, You could check out MarketLabInc.com. They supply Unique Hard-to-Find Products for the Clincal Laboratory. I've purchased several things from them (bins, plastic storage boxes, glove holders, document displays, etc.). Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: Ed Roy e...@illinois.edu To: histonet@lists.utsouthwestern.edu Sent: Tuesday, August 23, 2011 8:34 AM Subject: [Histonet] slide box freezer racks Hi I have a gazillion Fisher brand slide boxes that hold 50 slides each. We store the slides (fresh frozen unfixed) at -80 in freezer bags, but they are just piled into the freezer. Does anyone know of a source for a rack to organize such boxes in a -80 chest freezer? Thanks, Ed Roy -- Edward Roy, PhD Professor of Pathology Professor of Molecular and Integrative Physiology University of Illinois at Urbana-Champaign 506 S. Mathews Ave. Urbana, IL 61801 217 333-3375 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Antibody suggestions for dog and cat
Correction: I just found out that LabVision no longer sells the CD3 catalog number that I listed below; we also use CD3 catalog # RB-360 equally well on all species. Jan Shivers UMN VDL - Original Message - From: Jan Shivers shive...@umn.edu To: Mark Tarango marktara...@gmail.com; histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Sent: Monday, July 25, 2011 1:48 PM Subject: Re: [Histonet] Antibody suggestions for dog and cat Hi Mark, Vimentin - Dako; M0725, clone V9 Pankeratin - Dako; M0821; clone MNF116 CD3 - LabVision; RB-9039; rabbit polyclonal CD20 - LabVision; RB-9013; rabbit polyclonal CD18 (canine) - Leukocyte Antigen Biology Lab (UC-Davis; Dr. Peter Moore); clone CA16.3C10 CD18 (feline) - Leukocyte Antigen Biology Lab (UC-Davis; Dr. Peter Moore); clone FE3.9F2 Melan A - Dako; M7195; clone A103 Factor VIII - Dako; A0082; rabbit polyclonal CD117 - Dako; A4502; rabbit polyclonal (not specific for dogs; tested successfully on cow and horse, also) Let me know if you need information on any other antibodies in question. I species-validate all of my IHC tests (100+). Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: Mark Tarango marktara...@gmail.com To: histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Sent: Monday, July 25, 2011 1:33 PM Subject: [Histonet] Antibody suggestions for dog and cat Could anyone suggest suituble antibodies for the following markers in dog and cat tissue: Vimentin, Pankeratin, CD3 and CD20, CD18, MelanA, Factor VIII Also could use a suggestion for C-kit in Dog tissue only. thanks Mark ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Antibody suggestions for dog and cat
Hi Mark, Vimentin - Dako; M0725, clone V9 Pankeratin - Dako; M0821; clone MNF116 CD3 - LabVision; RB-9039; rabbit polyclonal CD20 - LabVision; RB-9013; rabbit polyclonal CD18 (canine) - Leukocyte Antigen Biology Lab (UC-Davis; Dr. Peter Moore); clone CA16.3C10 CD18 (feline) - Leukocyte Antigen Biology Lab (UC-Davis; Dr. Peter Moore); clone FE3.9F2 Melan A - Dako; M7195; clone A103 Factor VIII - Dako; A0082; rabbit polyclonal CD117 - Dako; A4502; rabbit polyclonal (not specific for dogs; tested successfully on cow and horse, also) Let me know if you need information on any other antibodies in question. I species-validate all of my IHC tests (100+). Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: Mark Tarango marktara...@gmail.com To: histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Sent: Monday, July 25, 2011 1:33 PM Subject: [Histonet] Antibody suggestions for dog and cat Could anyone suggest suituble antibodies for the following markers in dog and cat tissue: Vimentin, Pankeratin, CD3 and CD20, CD18, MelanA, Factor VIII Also could use a suggestion for C-kit in Dog tissue only. thanks Mark ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Macrophage marker in sheep for IHC
CD68 - clone KP1 has not worked on sheep (also not dogs, cows, horses, and chickens) in my experience. I have found it to stain primates well (and rare cells in pigs and cats). Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: Vivek Saroha vivek.sar...@nottingham.ac.uk To: histonet@lists.utsouthwestern.edu Sent: Friday, June 24, 2011 4:05 AM Subject: [Histonet] Macrophage marker in sheep for IHC Hi there, I am very new to basic sciences research and am planning an experiment to quantify and compare adipose tissue macrophage infiltration in sheep model using immunohistochemistry. I am specifically interested in CD11c as a marker of M1 activation of Macrophages. I have been advised that it is not easy and previous effort by my predecessors have not been very successful in this! From the general google, histonet/histosearch and pubmed search. I have made a list of potential candidates: 1.Clones PG M1 and Clone KP1 (Source : Histonet : http://www.histosearch.com/histonet/Nov03A/RE.HistonetSeepmacrophage.html) 2.EMB11, a mouse anti-human CD68 mAb (Dako, Carpenteria, CA) (source: published paper: http://www.ncbi.nlm.nih.gov/pubmed/16460804 ) 1. Another CD68 antibody Source: http://www.ingentaconnect.com/content/ocean/ajra/2011/0025/0002/art00024) 1. Iba1 (ionized calcium binding adaptor molecule 1) is a 17-kDa EF hand protein that is specifically expressed in macrophages/microglia and is upregulated during the activation of these cells.[Wikipedia] (source: I do not remember where I found this one from but Abcam has several antibodies none of which are predicted for sheep) I am writing to ask for suggestions about which one to go for and any other tips about searching for antibodies or performing IHC in paraffin fixed adipose tissue/liver. Any ideas will be gratefully appreciated as I am very new and isolated in this aspect. Best wishes Vivek Dr Vivek Saroha Clinical lecturer in Child health University of Nottingham This message and any attachment are intended solely for the addressee and may contain confidential information. If you have received this message in error, please send it back to me, and immediately delete it. Please do not use, copy or disclose the information contained in this message or in any attachment. Any views or opinions expressed by the author of this email do not necessarily reflect the views of the University of Nottingham. This message has been checked for viruses but the contents of an attachment may still contain software viruses which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] OT: How do you call...
When taking coursework eons ago, one of my professors addressed this very issue. He said that '-ic' and '-al' were BOTH adjective suffixes... and there was no need to include both at the end of a noun to turn it into an adjective (redundancy). So 'anatomic' it is. And it follows that another correct word spelling is 'pathologic', not 'pathological' though the latter is commonly used. Jan Shivers UMN - Original Message - From: Breeden, Sara sbree...@nmda.nmsu.edu To: histonet@lists.utsouthwestern.edu Sent: Monday, June 13, 2011 1:33 PM Subject: [Histonet] OT: How do you call... I've figured out how to fund my approaching retirement! A poll! So... is the correct term Anatomical Pathology or Anatomic Pathology? Send $5.00 with your email reply and I'll have the down payment for that tropical island with internet. Seriously - what is the consensus and/or the correct usage? Figure I better get all these Deep Questions answered while I have the chance. (P.S., I'm only kidding about the $5.00 but I do take PayPal). Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] MSDS info
If you have no luck with calling your vendors or finding an MSDS link on their website, simply Google your chemical name and the letters MSDS after it (such as: sodium chloride MSDS). Up will pop a long list of companies who have them available. Find the format you like the best and print out for your files. This should do, in lieu of one from the actual vendor, as long as the CAS number is the same as you use in the lab. Jan Shivers UMN - Original Message - From: Dianne E. Holmes dhol...@umc.edu To: Histonet@lists.utsouthwestern.edu Sent: Friday, May 27, 2011 11:47 AM Subject: [Histonet] MSDS info HELP !!! I am having to list all my chemicals in the lab for the Safety dept. Several have no MSDS sheet with them? (been here longer than I have!) Where can I obtain this info to put in my records? Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Coronavirus antibody
Hello, We have successfully stained ferret tissue using an antibody to Feline Infectious Peritonitis Virus (which also cross-reacts with swine Transmissible Gastroenteritis Virus). We buy our antibody from Custom Monoclonals, Inc. and use heat-induced epitope retrieval (pressure cooker and target retrieval solution). If you would like more details than this, I can send an uncontrolled copy of our SOP to you. Best regards, Jan Shivers Senior Scientist IHC/Histology/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: Chakib Boussahmain chak_...@yahoo.com To: histonet@lists.utsouthwestern.edu Sent: Tuesday, May 24, 2011 11:34 AM Subject: [Histonet] Coronavirus antibody Hi Histonetters, We are interesting in doing immunostain on ferret tissues that have been fixed in formalin and embedded in paraffin. I am just wondering if anyone used this antibody in their lab, if so, can you please share with us your staining protocol? antigen retrieval protocol as well? Your help will be very much appreciated. Thank you Chakib HTL(ASCP) Histology lab MIT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC pos. neg. control question
I agree 100% with Glen. Jan Shivers UMN VDL - Original Message - From: Dawson, Glen gdaw...@dynacaremilwaukee.com To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 19, 2011 1:32 PM Subject: RE: [Histonet] IHC pos. neg. control question IMHO: Running any piece of tissue as a control that does not belong to the patient being tested makes zero sense. Because it would not be from the patient tissue being tested, how do you know if it was handled the same as the patient tissue? For example: 1) Were they processed the same way? 2) Did the patient tissue dry out in the OR before it was delievered? 3) Was the patient tissue ever irradiated? 4) Does the patient tissue contain any of a number of substances that could cause non-specific staining. 5) Was the patient abducted by aliens? My point is that running a piece of tissue as a negative control that is not even from the patient being tested throws all of the conditions that the patient tissue was exposed to prior to and during processing out the window. This makes NO sense. Glen Dawson BS, HT(ASCP) QIHC IHC Manager Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Curt Tague Sent: Thursday, May 19, 2011 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC pos. neg. control question I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control. The patient's own tissue cannot be used as a negative control. The tissue that stained positively must serve as the negative control without the antibody. This is critical and you need to correct that immediately. Curt ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] esterase stain
Good Morning, I'm posting this for a non-member. Does anyone know of a lab that does nonspecific esterase staining? A client needs it done on mouse tissue, and we don't perform that test here in our histo lab. Jan Shivers University of Minnesota Veterinary Diagnostic Laboratory shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Fw: [Histonet] CD79 alpha dilemma - ANSWER CORRECTION
Hello everyone - my apologies for posting a partially incorrect answer last week. I was looking at the wrong line on a spreadsheet for CD79a abbreviated protocol information and wrote down the wrong HIER information. What we do here currently with HIER for Biocare's CD79a (clone HM47/A9) on dogs, cats, pigs, cows, etc., is unmask antigens using: 0.1M Citrate buffer, pH 6.0 in a Biocare Decloaking Chamber. (High pH retrieval solution does expose more antigenic sites, but it damages the tissue more when using the pressure cooker method.) In the past we used high pH retrieval solution when doing HIER using a microwave oven (gentler method). Jan Shivers UMN Vet Diag Lab - Original Message - From: Jan Shivers shive...@umn.edu To: Farish, Craig cfar...@csu.edu.au; histonet@lists.utsouthwestern.edu Sent: Friday, May 13, 2011 9:14 AM Subject: Re: [Histonet] CD79 alpha dilemma Hi Craig, I have found CD79a from Biocare (cat. # CM067C) to stain dog, cat, pig, cow, horse, sheep, and mouse so far. Have not tried it on other species yet (in other words, I don't have any negative species results to report). I do have to use HIER with high pH retrieval buffer (Dako; contains EDTA) to expose antigens (in a Biocare Decloaking Chamber/pressure cooker). Citrate buffer doesn't do the job well enough. Microwave oven antigen retrieval will also work, but it exposes fewer epitopes than the pressure cooker. If you have any more questions, feel free to contact me directly. Jan Shivers Senior Scientist IHC/Histology/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: Farish, Craig cfar...@csu.edu.au To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 12, 2011 8:35 PM Subject: [Histonet] CD79 alpha dilemma Hi folks - has anyone out there found a Cd79α clone and supplier which will cover multiple species reliably? I can find clones which will stain cats and dogs (and humans), and clones which will cover horses, pigs, cows and primates (and humans, but not opossums for some reason??) but i'm yet to find one which will cross-react with both the large and small animals commonly encountered in a vet lab. I would rather not have to stock 2 versions of the same antibody if I can avoid it. Any suggestions would be much appreciated. As always, thanks to everyone who contributes to histonet, Craig Craig Farish Senior Technical Officer Veterinary Diagnostic Laboratory School of Animal and Veterinary Sciences Charles Sturt University Boorooma Street Wagga Wagga NSW 2678 Australia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD79 alpha dilemma
Hi Craig, I have found CD79a from Biocare (cat. # CM067C) to stain dog, cat, pig, cow, horse, sheep, and mouse so far. Have not tried it on other species yet (in other words, I don't have any negative species results to report). I do have to use HIER with high pH retrieval buffer (Dako; contains EDTA) to expose antigens (in a Biocare Decloaking Chamber/pressure cooker). Citrate buffer doesn't do the job well enough. Microwave oven antigen retrieval will also work, but it exposes fewer epitopes than the pressure cooker. If you have any more questions, feel free to contact me directly. Jan Shivers Senior Scientist IHC/Histology/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: Farish, Craig cfar...@csu.edu.au To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 12, 2011 8:35 PM Subject: [Histonet] CD79 alpha dilemma Hi folks - has anyone out there found a Cd79α clone and supplier which will cover multiple species reliably? I can find clones which will stain cats and dogs (and humans), and clones which will cover horses, pigs, cows and primates (and humans, but not opossums for some reason??) but i'm yet to find one which will cross-react with both the large and small animals commonly encountered in a vet lab. I would rather not have to stock 2 versions of the same antibody if I can avoid it. Any suggestions would be much appreciated. As always, thanks to everyone who contributes to histonet, Craig Craig Farish Senior Technical Officer Veterinary Diagnostic Laboratory School of Animal and Veterinary Sciences Charles Sturt University Boorooma Street Wagga Wagga NSW 2678 Australia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] need monkey control tissue
Hello, I'm posting this request for a colleague, who is in need of normal monkey placenta (preferably Rhesus). If anyone has some to spare, please let me know and I'll forward your response. Thanks, Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Processing animal fat
We trim to 2mm thick. Jan Shivers UMN Vet Diag Lab - Original Message - From: Liz Chlipala l...@premierlab.com To: Sherwood, Margaret msherw...@partners.org; histonet@lists.utsouthwestern.edu Sent: Thursday, March 17, 2011 3:02 PM Subject: RE: [Histonet] Processing animal fat 5mm is pretty thick still. We process skin and fat and we use a longer processing cycle. 1 to 1.5 hours per station. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Thursday, March 17, 2011 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Processing animal fat My question is directed specifically to veterinary histologists or histologists who also do a fair amount of animal processing. We are having a terrible time processing pig fat. We had problems previously, but thought we had solved them. This latest project (pig skin with a lot of fat attached) came out awful. The fat was not adequately processed: couldn't section it, it just crumbled. In the block, it appears white and crumbly. The funny thing is some blocks came out all right, but most didn't. PLEASE help! Let me know how you process your animal fat (sp. Pig)! Is there a size issue (we trim it if it is greater than 5mm)? We have gotten help from the histonet before and instituted these suggestions (i.e. let sit in formalin for 48 hours; put in cassettes in a 60 degree oven for 1-2 hours and then cool to room temp. Then process using a 1 hour program). Any help would be appreciated. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Fw: SPAM-LOW: RE: [Histonet] about ki-67
Patsy, et al... NeoMarkers' antibody is the same one as ThermoScientific (TS owns NeoMarkers/LabVision antibodies). Jan Shivers - Original Message - From: Patsy Ruegg pru...@ihctech.net To: 'Ross Benik' r...@premierlab.com; 'Mehmet Fatih BOZKURT' fbozk...@gmail.com; Histonet@lists.utsouthwestern.edu Sent: Monday, February 21, 2011 1:16 PM Subject: RE: SPAM-LOW: RE: [Histonet] about ki-67 I have not tried it on bovine but the NeoMarkers Rabbit monoclonal Ki67 has worked on every other species I have tried, rat, mouse, human, guinea pig, canine. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ross Benik Sent: Monday, February 21, 2011 12:00 PM To: Mehmet Fatih BOZKURT; Histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: RE: [Histonet] about ki-67 Hi Mehmet, We have used the Ki-67 antibody body from Santa Cruz Biotechnology (sc-15402, rabbit poly) on multiple species such as human, rat, mouse and canine. We have not had the opportunity to test its cross reactivity with bovine but it may be your best chance. -Ross -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mehmet Fatih BOZKURT Sent: Monday, February 21, 2011 11:28 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] about ki-67 Hello, This is the first mail that i sent to this group. I'm looking for Ki-67 primary antibody for use on bovine tissue. I couldn't find on the internet search. Does anyone know if there is a ki-67 primary antibody for available IHC on bovine tissue ? Best Regards, Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-109 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Fw: [Histonet] about ki-67
I neglected to mention that in my experience ThermoScientific's antibody also works on dog, cat, pig, horse, rat, and primate; it does not work on birds. Jan Shivers Univ. of Minnesota - Original Message - From: Jan Shivers shive...@umn.edu To: Mehmet Fatih BOZKURT fbozk...@gmail.com; Histonet@lists.utsouthwestern.edu Sent: Monday, February 21, 2011 12:58 PM Subject: Re: [Histonet] about ki-67 Hello, Dr. Bozkurt, I know that Ki-67 from ThermoScientific (cat# RB-9043) works on bovine tissue, in my experience. It does not stain a large quantity of cells in bovine tonsil, but those that do stain positively stain with moderate intensity. It is a rabbit polyclonal antibody. Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: Mehmet Fatih BOZKURT fbozk...@gmail.com To: Histonet@lists.utsouthwestern.edu Sent: Monday, February 21, 2011 12:28 PM Subject: [Histonet] about ki-67 Hello, This is the first mail that i sent to this group. I'm looking for Ki-67 primary antibody for use on bovine tissue. I couldn't find on the internet search. Does anyone know if there is a ki-67 primary antibody for available IHC on bovine tissue ? Best Regards, Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-109 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: SPAM-LOW: RE: [Histonet] about ki-67
Patsy, This particular ThermoScientific/NeoMarkers catalog number (RB-9043) is a rabbit polyclonal. Jan - Original Message - From: Patsy Ruegg pru...@ihctech.net To: 'Jan Shivers' shive...@umn.edu; 'histonet' histonet@lists.utsouthwestern.edu Sent: Monday, February 21, 2011 1:29 PM Subject: RE: SPAM-LOW: RE: [Histonet] about ki-67 Yes you are right NeoMarkers is Thermo, but I am pretty sure it is a Rabbit Monoclonal isn't it? Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Monday, February 21, 2011 12:23 PM To: histonet Subject: Fw: SPAM-LOW: RE: [Histonet] about ki-67 Patsy, et al... NeoMarkers' antibody is the same one as ThermoScientific (TS owns NeoMarkers/LabVision antibodies). Jan Shivers - Original Message - From: Patsy Ruegg pru...@ihctech.net To: 'Ross Benik' r...@premierlab.com; 'Mehmet Fatih BOZKURT' fbozk...@gmail.com; Histonet@lists.utsouthwestern.edu Sent: Monday, February 21, 2011 1:16 PM Subject: RE: SPAM-LOW: RE: [Histonet] about ki-67 I have not tried it on bovine but the NeoMarkers Rabbit monoclonal Ki67 has worked on every other species I have tried, rat, mouse, human, guinea pig, canine. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ross Benik Sent: Monday, February 21, 2011 12:00 PM To: Mehmet Fatih BOZKURT; Histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: RE: [Histonet] about ki-67 Hi Mehmet, We have used the Ki-67 antibody body from Santa Cruz Biotechnology (sc-15402, rabbit poly) on multiple species such as human, rat, mouse and canine. We have not had the opportunity to test its cross reactivity with bovine but it may be your best chance. -Ross -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mehmet Fatih BOZKURT Sent: Monday, February 21, 2011 11:28 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] about ki-67 Hello, This is the first mail that i sent to this group. I'm looking for Ki-67 primary antibody for use on bovine tissue. I couldn't find on the internet search. Does anyone know if there is a ki-67 primary antibody for available IHC on bovine tissue ? Best Regards, Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-109 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section
Abcam has a rabbit anti-neutrophil elastase that works on rat FFPE tissues, according to their website. Jan Shivers - Original Message - From: Patsy Ruegg pru...@ihctech.net To: 'Amy Lee' amylee...@yahoo.com; 'histonet' histonet@lists.utsouthwestern.edu Sent: Thursday, February 17, 2011 8:49 AM Subject: RE: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section I do not know of a neutrophil specific ab for human ffpe tissue. You could do just DAB without blocking with h202 for myleoperoxidase but it will not distinguish between eosinophils and neutrophils myeloperoxidase will stain all myeloid cells with granules in the cytoplasm (granulocytes). Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Lee Sent: Wednesday, February 16, 2011 1:07 PM To: histonet Subject: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section Hello, I was asked to perform neutrophil IHC on rat FFP sections. I searched around and found most are for mouse tissue. There is one from Serotec mouse anti rat but it does not work on paraffin section. Could anybody recommend a good antibody? Thanks in advance, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section
The website says it works on humans. I have not tried this particular Ab
myself, though I have tried another xNeutrophil Elastase.
Jan Shivers
- Original Message -
From: Patsy Ruegg pru...@ihctech.net
To: 'Jan Shivers' shive...@umn.edu; 'Amy Lee' amylee...@yahoo.com;
'histonet' histonet@lists.utsouthwestern.edu
Sent: Thursday, February 17, 2011 12:20 PM
Subject: RE: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section
Does it also work on human?
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pru...@ihctech.net
web site www.ihctech.net
This email is confidential and intended solely for the use of the
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Accordingly
any dissemination, distribution, copying, or other use of this message, or
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If
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-Original Message-
From: Jan Shivers [mailto:shive...@umn.edu]
Sent: Thursday, February 17, 2011 9:39 AM
To: Patsy Ruegg; 'Amy Lee'; 'histonet'
Subject: Re: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section
Abcam has a rabbit anti-neutrophil elastase that works on rat FFPE
tissues,
according to their website.
Jan Shivers
- Original Message -
From: Patsy Ruegg pru...@ihctech.net
To: 'Amy Lee' amylee...@yahoo.com; 'histonet'
histonet@lists.utsouthwestern.edu
Sent: Thursday, February 17, 2011 8:49 AM
Subject: RE: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section
I do not know of a neutrophil specific ab for human ffpe tissue. You
could
do just DAB without blocking with h202 for myleoperoxidase but it will not
distinguish between eosinophils and neutrophils myeloperoxidase will stain
all myeloid cells with granules in the cytoplasm (granulocytes).
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net
www.ihcrg.org
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Lee
Sent: Wednesday, February 16, 2011 1:07 PM
To: histonet
Subject: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section
Hello,
I was asked to perform neutrophil IHC on rat FFP sections. I searched
around
and found most are for mouse tissue. There is one from Serotec mouse anti
rat but it does not work on paraffin section.
Could anybody recommend a good antibody?
Thanks in advance,
Amy
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[Histonet] veterinary IHC for Ornithobacterium
Does anyone do IHC staining for Ornithobacterium rhinotracheale (ORT), a respiratory pathogen in poultry? I'm trying to find a lab to do it, or a vendor source for antibody. Much thanks in advance, Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Stupid, stupid static!!
If you use lotion in the lab, won't it get on your slides and cause havoc (big oil droplets)? Jan Shivers - Original Message - From: Burton, Lynn lynn.bur...@illinois.gov To: sgoe...@mirnarx.com; Histonet@lists.utsouthwestern.edu Sent: Wednesday, January 05, 2011 1:21 PM Subject: [Histonet] RE: Stupid, stupid static!! Use fabric softener sheets. You can wipe the blades and knife holders with them. My daughter's American Girl magazine says to pour lotion in your hand, rub it between your hands and while it is still shiny run your hands through your hair to control static. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@mirnarx.com [sgoe...@mirnarx.com] Sent: Wednesday, January 05, 2011 1:15 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Stupid, stupid static!! So where the microtome is here that I have to use we have to wear those blue hospital booties and disposable lab coats (the white paper type ones). With me and several other people walking around in those booties the amount of static electricity is to say the least frusterating!! Does anyone know of anything I can do to get rid of the static? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] norepinephrine IHC
I'm posting this request for someone who is not a member of Histonet - Does anyone have a lab that performs norepinephrine IHC testing on species other than human? If so, do you take outside clients? Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] negative controls
Linda, My system of negative controls is identical to yours. Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: Sebree Linda A lseb...@uwhealth.org To: sgoe...@xbiotech.com; Rene J Buesa rjbu...@yahoo.com Cc: Histo Net list server HistoNet@lists.utsouthwestern.edu Sent: Friday, October 15, 2010 11:07 AM Subject: RE: [Histonet] negative controls To clarify even further: we cut 2 patient sections, put one on a slide with a section of positive control already on it. This slide gets stained with the antibody The other section goes on another slide and is run as a corresponding negative control using the same antibody protocol but substituting a negative control serum for the antibody, thus this is a negative reagent control slide. Elements within the patient slide that received antibody and are expected to be negative, serve as a negative tissue control. Again, we run 1 negative control slide for EVERY 1 patient block in a run but only 1 negative control per any number of antibodies run on that same block, using the harshest protocol. Only autopsy cases differ in that we run 1 negative control per TISSUE TYPE. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: sgoe...@xbiotech.com [mailto:sgoe...@xbiotech.com] Sent: Friday, October 15, 2010 10:48 AM To: Rene J Buesa Cc: Histo Net list server; Sebree Linda A Subject: RE: [Histonet] negative controls So for every HP you do, you process a control cassette with the patient tissue cassette? That seems like alot? How do you get that many control tissues on a daily basis? What do you do with the remaining tissue in the control block? If you throw them away everyday, I would be interested in some of them. How do you know what IHC stains the pathologist is going to order to know what control tissue to fix and process at the exact same time? We have always just had a bunch of blocks that you cut a control from? I understand that there is variability with processing, age, etc. not trying to be dense just still don't understand... Most places I have ever worked have control blocks that they cut a fresh control from everyday, then stain with the patient tissue. If there are 3 HP cases, from what I am understanding, you guys are saying you need 3 controls for slides that are on the same machine, with the same reagents, same antibody, and same times. Why couldn't you just have one for all 3 cases? Then the next day have a fresh ONE for that day, date them, and file them. So if you needed to see the HP control for October 15th, you could go pull the control for that day... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 Original Message Subject: RE: [Histonet] negative controls From: Rene J Buesa rjbu...@yahoo.com Date: Fri, October 15, 2010 8:33 am To: Sebree Linda A lseb...@uwhealth.org, sgoe...@xbiotech.com Cc: Histo Net list server HistoNet@lists.utsouthwestern.edu Because each tissue block has its own characteristics regarding fixation and processing some of which can influence the reactivity. If you have a bank of negative controls, how can you be sure that any of those blocks have received exactly the same treatment and reacted in the same way to the test block? The same goes for any bank of positives, so that is why you should have a positive control section in the same slide as the test section. René J. --- On Fri, 10/15/10, sgoe...@xbiotech.com sgoe...@xbiotech.com wrote: From: sgoe...@xbiotech.com sgoe...@xbiotech.com Subject: RE: [Histonet] negative controls To: Sebree Linda A lseb...@uwhealth.org Cc: Histo Net list server HistoNet@lists.utsouthwestern.edu Date: Friday, October 15, 2010, 11:17 AM Why do you need a negative control for each block if you are runn= ing the same antibody on each patient block? Is it just for case by c ase reference so the negative is filed with the patient slide? Why co= uldn't you have a control slide bank that was dated so all the slides you d= id on that day, on that run, could be referenced back to that control? = ; Just curious? Sarah Goebel, B.A., HT (ASCP) Histotechnician= br XBiotech USA Inc. 8201 East Riverside Dr. Bld= g 4 Suite 100 Austin, Texas 78744
Re: [Histonet] Immunohistochemistry images
Try IHCWORLD Image Gallery. http://www.ihcworld.com/imagegallery/ Jan Shivers UMN VDL - Original Message - From: Michele Carr michelecar...@yahoo.com To: histonet@lists.utsouthwestern.edu Sent: Thursday, October 14, 2010 10:40 AM Subject: [Histonet] Immunohistochemistry images Hi everyone, I was wondering if anyone knew of a website that I can view and save the IHC images. We are putting together a new procedure manual and the pathogists want images of the antibodies we use to be included in the manual. Thanks in advance for your responses. Michele Carr HTL ASCP Medical Laboratory Services Murrieta Ca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] problems with staining IHC
Mala, If your main problem is that the reagents aren't spreading across your slides, I agree that you should put Tween 20 (0.05% per volume) in all of your rinse buffers between steps, regardless of if you do your IHC stains manually or with an autostainer. If this doesn't solve the problem, post your method on histonet so that we know more about your procedure steps and can send additional advice. Jan Shivers UMN Vet Diag Lab - Original Message - From: sris...@mail.holyname.org To: histonet-boun...@lists.utsouthwestern.edu; Histonet@lists.utsouthwestern.edu Sent: Thursday, October 07, 2010 9:32 AM Subject: [Histonet] problems with staining IHC Hi All, Our pathologists have made a complain that the staining of slides have been a problem. Same antibodies which stain one day does not work the following day. This has been going on for a few months. When we repeat stain they either work or not work and we have been sending them to reference labs. Our tech support person was here and told us that the slides ( fisher plus slides) have been a problem with some of her customers. Meanwhile our surrounding labs which are using the same slides have no issues. She showed us how the reagents were not spreading properly on the slides. Is anyone confronted this issue? If so, how was it solved? Thanks in advance. Mala Srishan Supervisor, Histology Holy Name Medical Center Teaneck NJ 07666 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] WNV IHC
Hello everyone, A few people asked me to post some information on my West Nile Virus IHC test method, so here are some general comments. If you'd like more detailed information, please write to me directly. Tissue pretreatment - pressure cooker (Biocare Decloaking Chamber) with Target Retrieval solution (Dako) Primary antibody - anti-West Nile Virus (BioReliance, cat. # 81-002; clone 7H2; working dilution at 3-6 ug/ml Ab concentration, depending upon lot) Secondary linking antibody - EnVision+/HRP goat anti-mouse IgG (Dako; with 2-4% normal case species serum added per volume) Chromogen - AEC+ (Dako) Best regards, Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: Re-thinking Nigerian Scam ! [Histonet] FW: NEEDS URGENT HELP !!!
Reminder... these 'please send money' or 'please send your bank acct information so that I can deposit money into your acct' pleas are ALWAYS scams. They have been for years. If a trusted 'friend' was truly stranded in a foreign country, they'd borrow a phone and call home. If a long lost relative died and left you money in another country, it would be a lawyer contacting you via telephone or letter, and definitely not through an email message. Best advice... don't even open these messages up. They're bogus, and could be carrying viruses, worms, etc. themselves. Being on a listserv like histonet, we're prone to receive a lot of these email fraud attempts. Ignore them. Jan Shivers - Original Message - From: Akemi Allison akemiat3...@yahoo.com To: histonet@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu; Valantou Grover vgro...@polysciences.com Sent: Tuesday, August 17, 2010 8:24 PM Subject: Re: Re-thinking Nigerian Scam ! [Histonet] FW: NEEDS URGENT HELP !!! Sorry Histonetters, but this needs to be cleared up! OK, Jerry Santiago, are you alive and well in FL or the UK in trouble, or is this another ridiculous scam I know how well connected you are, so one of your colleagues should know... Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Aug 17, 2010, at 1:47 PM, Eric Hill wrote: Hi, My wife got scammed with something almost the same a month ago, less detailed message in hers though! They got into her gmail and deleted all her contacts and tried to delete all her most recent emails (they forgot to clear out her trash though, so she got most back). Don't fall for it! -Eric Research Tech 1 Program in Memrane Biology Mass Genreal Hospital On Aug 17, 2010, at 4:37 PM, Akemi Allison wrote: I am rethinking this email since I read it over again. The scam email I received asked for money ($1,800) to be sent to England via Western Union. I am not sure about this since this supposed Jerry is asking for just a response email. I would still be very cautious! The Idaho news said the lady received a yahoo request (same as mine) asking for email address, password, country of origin, mothers maiden name, ect. Once the perpetrator had the information, they immediately extracted all her information and cleaned her bank accounts out. This was a business computer account and it shut down her business! Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Aug 17, 2010, at 1:20 PM, Valantou Grover wrote: Thanks, I deleted the message, now I am going to run a anti virus program. Valantou ** * Visit us at the 36th Annual National Society for Histotechnology Convention - September 26-28, Seattle, WA - BOOTH 126-128 * Polysciences/Bangs Laboratories, Inc. will be hosting the 2010 Latex Course in Orlando, FL at the Grand Floridian Resort from 10/3 – 10/5. Please enquire at i...@bangslabs.com for additional details! Phone: 800-387-0672, 317-570-7020. * From: Akemi Allison [mailto:akemiat3...@yahoo.com] Sent: Tuesday, August 17, 2010 4:14 PM To: Valantou Grover Cc: histonet@lists.utsouthwestern.edu Subject: Re: Nigerian Scam ! [Histonet] FW: NEEDS URGENT HELP !!! The email you received is a Nigerian Scam. Someone was able to hack into Jerry's computer and get all his contact list! You happened to be one of his contacts. The same sort of Scam happened to me last October. Fortunately for me, I didn't have any bank account information in my computer. I have a MAC and so I went to the Apple Genius Bar and they checked my computer for any viruses. Fortunately, it is very difficult to get into a MAC. This has been on the news in Idaho and various other states. DO NOT RESPOND to any emails that want any personal information!!! You need to clean-up your computer from any viruses. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Aug 17, 2010, at 12:53 PM, Valantou Grover wrote: I am just curious, can someone check on Jerry to see if he is dead or alive, or if someone stole his phone/laptop to get this message to me. I remember the email address from Florida Society for Histotechnology, I am wondering if this is real or if someone is assuming his identity overseas, foul play, etc. Valantou Grover, Ph.D. HT/HTL(ASCP), PA, MBA Biosciences Product Line Manager Polysciences, Inc. 400 Valley Road Warrington, PA 18976 Fax: 1-800-343-3291 Phone number: 1-800-523-2575 X7418 Direct:1-215-488-7418 Cell phone: 1-215
Re: [Histonet] FW: bovine viral diarhea antibody
VMRD Mouse monoclonals - Clone D89 and clone BA-2 are now working stronger than my previous clone (15C5 from another vendor). Both need heat retrieval. Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: Burton, Lynn lynn.bur...@illinois.gov To: histonet@lists.utsouthwestern.edu Sent: Wednesday, June 30, 2010 9:48 AM Subject: [Histonet] FW: bovine viral diarhea antibody I am looking for a vendor of BVDV antibody for IHC testing. We had used Syracuse Bioanalytical in the past. They seem to be gone. I would appreciate any suggestions. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Porcine Hemagglutinating Encephalomyelitis Virus IHC
Does anyone know of a commercial source for unconjugated anti-porcine hemagglutinating encephalomyelitis virus that can be used on FFPE tissues? Thanks in advance, Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Porcine Hemagglutinating Encephalomyelitis Virus IHC
Causal agent - a neurotropic coronavirus of young pigs; characterized by vomiting and wasting disease and/or motor disorders (incoordination, convulsions ) due to acute encephalomyelitis (which can lead to death within days). Pigs are the only known natural host of the virus. Jan Shivers - Original Message - From: Thomas Britten tbrit...@aol.com To: Jan Shivers shive...@umn.edu Cc: histonet histonet@lists.utsouthwestern.edu Sent: Monday, June 14, 2010 12:28 PM Subject: Re: [Histonet] Porcine Hemagglutinating Encephalomyelitis Virus IHC What is this virus. Sent from my iPhone On Jun 14, 2010, at 12:21 PM, Jan Shivers shive...@umn.edu wrote: Does anyone know of a commercial source for unconjugated anti- porcine hemagglutinating encephalomyelitis virus that can be used on FFPE tissues? Thanks in advance, Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] possible AFB contaminant
How often are you decontaminating your NeXes machines (running decontaminating reagents through the tubing)? Jan Shivers UMN VetDiagLab - Original Message - From: debra.or...@uchospitals.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 20, 2010 2:41 PM Subject: [Histonet] possible AFB contaminant Please help, we have had an increase in demands for repeats on our AFB stain. Our pathologists have been noticing what they believe are false positives on our slides. We perform our AFBs on the Ventana Nexus and some are done by hand. Because of this problem, we have been doing side by side comparisons using the Nexus and hand stain. We still have complaints. We have made new reagents, changed kits, tested our DI water sytem and have different techs cutting using different waterbaths. Any advice or comments would be greatly appreciated. Thanks Debi This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cd31 in pigs
Von Willebrand Factor (Factor VIII) is also what I use to stain pig endothelium. Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: Jean-Martin Lapointe jm.lapoi...@accellab.com To: histonet@lists.utsouthwestern.edu Sent: Friday, April 09, 2010 10:59 AM Subject: [Histonet] Cd31 in pigs Hi Joel, we have tried a few antibodies for CD31 in FFPE tissues in pigs and have had no luck. We currently use an antibody to vWF to stain pig endothelium. If someone has a working technique for CD31 in swine, I'd be happy to hear about it. Jean-Martin __ Jean-Martin Lapointe, DMV, MS, dACVP AccelLAB Inc Québec, Canada J7H 1N8 jm.lapoi...@accellab.com Date: Fri, 9 Apr 2010 09:40:00 -0400 From: Joel Israel jo...@mcclainlab.com Does anyone have a procedure that WORKS for cd31 in pigs? I can't seem to get it to work no matter what I do. Any help will be greatly appreciated. Thanks.- joel ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Anti-human abs work on porcine tissue
I work on all species, porcine being one of them. I've never done a % survey of the number of Abs made against human epitopes that also work on porcine tissue, but I can give you generalizations. All of the rabbit polyclonal antibodies that I've tried have cross-reacted with porcine tissue. The polyclonals that I use the most are the Abs against hormones, neuroendocrine markers, vasculature, astrocytes, etc. Most of the mouse monoclonal antibodies that I've tried have cross-reacted with porcine tissue. These would be the intermediate filaments, melanoma antigens, cell proliferating antigens, neuroendocrine markers, etc. You may have more difficulty with leukocyte markers (CD markers). They seem to have less homology with human markers than the above items, and not all cross-react, in my experience. You only learn this by trial and error, unfortunately, since data about species cross-reactivity is rare. I have 2 polyclonal and 2 monoclonal CD markers that cross-react with porcine. That is not to say that there aren't more, but that's all that I currently have worked up on porcine tissue. I have not tried Abs like ER, PR, CEA, TTF, etc. I've done species cross-reactivity studies with every antibody I have in stock (80), so if you send me a list of the antibodies you're interested in (directly to me), I can let you know which ones cross-react with porcine tissue, and which vendor works for me. Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: RICKY MATHIS cmmath...@bellsouth.net To: histonet histonet@lists.utsouthwestern.edu Sent: Wednesday, March 17, 2010 8:15 PM Subject: [Histonet] Anti-human abs work on porcine tissue I work with some porcine tissues and it is sometimes difficult to find antibodies specific to pig. A doctor I work with asked some one he knew in Europe about using antibodies that are listed as working in human being used on porcine tissue. This person stated that there would be about a 10% chance that the anti-human antibodies would work on porcine tissues. The antibody companies mostly give the same we did not try it on pig tissue response. I understand that it is likely expensive to continue to test antibodies on a wide variety of animal tissues, so that is fine. But what do you guys think about the 10% chance? I would have said more than that. Thank you in advance for your time, Cathy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Stain for Smooth Muscle Cells
Smooth Muscle Actin; Dako; cat. # M0851, clone 1A4 Requires HIER antigen retrieval (I use a Biocare Decloaking Chamber with Dako Target Retrieval Solution) Strong staining on sheep tissue Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: Sherwood, Margaret msherw...@partners.org To: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 17, 2010 8:49 AM Subject: Re: [Histonet] Stain for Smooth Muscle Cells Hi all, What stain do most people use to demonstrate smooth muscle cells? I know there are various choicesWe are looking at sheep lung. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC protocol for vimentin in animal tissue
Lynn, I don't know anything about the Ventana Benchmark XT requirements, but I have never had any problem with Vimentin on a Dako Autostainer and also done manually on all sorts of animal tissue - with the exception of mouse. The V9 clone doesn't work on mouse, in my experience. (It'll work on frogs, however; go figure.) My protocol uses Dako Target Retrieval in a Biocare Decloaking Chamber for antigen retrieval, 45 min in primary antibody, 30 min in EnVision+/HRP goat anti-mouse IgG, and 10 minutes in AEC. Very strong staining. Hope you get the specific answers you need from others. Jan Shivers UMN Vet Diag Lab - Original Message - From: Burton, Lynn lynn.bur...@illinois.gov To: histonet@lists.utsouthwestern.edu Sent: Tuesday, February 02, 2010 12:50 PM Subject: [Histonet] IHC protocol for vimentin in animal tissue Does anyone out there have an IHC protocol, for Vimentin, in animal tissues, that has been working for them on the Ventana Benchmark XT? I would appreciate any suggestions at this point. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] veterinary IHC question - BVD Ab
Hi all, I've been using the BVD clone 15C5 for years, but need to find another vendor source with reactivity to another clone. Does anyone have experience with and recommendations for a commercial source of a monoclonal BVD antibody that has strong reactivity? This would be for Type 2 BVD virus. Thanks in advance, Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] shipping slides, elementary question
I pack slides in 100-slide plastic boxes, with gauze sponges laid inside the boxes on top of slide edges to ensure that they don't jiggle around inside the box. Boxes are taped shut, then packed inside a cardboard box with plenty of room for packing peanuts or bubble wrap. Never have had a problem. Jan Shivers - Original Message - From: Nicole Collette collet...@mail.llnl.gov To: histonet@lists.utsouthwestern.edu Sent: Friday, January 08, 2010 12:28 PM Subject: [Histonet] shipping slides, elementary question Happy Friday everyone, I have a very basic question about shipping slides (mouse tissue, non-biohaz). I am planning to ship a bunch to a collaborator, on the order of a few hundred. I have black hinged cardboard/wood 100-slide boxes, similar to https://www.vwrsp.com/catalog/product/index.cgi?catalog_number=48452-001inE=1highlight=48452-001 will these be sufficient for shipping (provided I ensure they stay closed during transit) to avoid breakage? I have slide mailers, but they only hold a few slides. I will do that if I need to (it would be a whole lot of packaging and labeling though...), but don't want to just send a giant box and have them all broken on the other end. Maybe there's another alternative? I'm sure someone on histonet has done this before :) Thanks in advance for the advice. Sincerely, Nicole Collette Lawrence Livermore National Lab/ UC Berkeley collet...@llnl.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Slide Labelers
Leica IP S Slide Printer - Original Message - From: Igor Deyneko igor.deyn...@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Friday, December 18, 2009 10:44 AM Subject: [Histonet] Slide Labelers Dear Histonetters! I was wondering if anyone out there is using a slide labeler. I would appreciate any info on models(serial numbers), and vendors. We have high throughput and need a more automated system with minimal time to input data. Thank you for any suggestions. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] veterinary lab question - chem expiration
What are veterinary labs (non-CAP inspected labs) setting as their expiration dates for dry and liquid chemicals that come without expiration dates on the labels? On the Histonet I've found varying answers from human labs (5-10 yrs for dry; 1-5 yrs for liquid). I'm in the process of setting guidelines for my labs, but want to be sure to be in agreement with other vet labs out there as well. Thanks, Jan Shivers Senior Scientist Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] buffer protocols
http://www.ihcworld.com/_protocols/epitope_retrieval/citrate_buffer.htm Jan Shivers - Original Message - From: Justin Peters jpet...@bostwicklaboratories.com To: histonet@lists.utsouthwestern.edu Sent: Thursday, December 03, 2009 3:36 PM Subject: [Histonet] buffer protocols Can someone help me with a protocol for making citrate buffer (pH 6.0) for IHC antigen retrieval? Justin Peters, HTL (ASCP) IHC Supervisor Bostwick Laboratories(tm) For Absolute Confidence(r) 4355 Innslake Drive Glen Allen, Virginia 23060 Phone:(804) 967-9225 ext. 1831 Cell:(804) 822-6084 Email: jpet...@bostwicklaboratories.com mailto:jpet...@bostwicklaboratories.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] GFAP IHC
Nancy, I don't use the ready-to-use GFAP from Dako, but I do use the concentrated form of GFAP (it is also a rabbit anti-cow GFAP.) It's worked successfully in dog, cat, pig, cow, horse, goat, sheep, deer, eagle, mouse, rat, gerbil, guinea pig, hamster, monkey, ferret, and kangaroo with no background problems whatsoever. The EnVision+HRP Rabbit will only bind to the rabbit IgG, not the cow IgG, so that is not the source of your background. I would suspect that if you're getting brown staining on the slide itself, your antibody is much much too concentrated. My working concentration calculates out to be 4 ug/ml of Ab. You might see if your ready-to-use preparation has a concentration listed, and then work from there to approximate a 4 ug/ml concentration with diluting it out. If that doesn't work... buy the concentrated stock form and optimize it for your tissues. In my lab, it requires absolutely no tissue pretreatment, and the stain is always strong, clear and clean. Good Luck! Best regards, Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: Nancy Herman nancy.her...@inspection.gc.ca To: histonet@lists.utsouthwestern.edu Sent: Wednesday, October 28, 2009 3:19 PM Subject: [Histonet] GFAP IHC We are using DAKO's ready-to-use GFAP antibody on our bovine tissue and using Envision+ HRP Rabbit detection system. When using this antibody we see a brown color on the slide itself. The staining was intense and quite uneven. We have diluted the anitbody to 1:10 but there is still the brown slide. The staining is not quite as uneven but appears more washed out. We don't see this with any of our other antibodies we are using and our no primary controls for GFAP are clean. Any advice or explanations for the slides picking up the brown color or improving GFAP IHC on bovine tissue? Thanks Nancy Herman CFIA Lethbridge Laboratory Lethbridge, Alberta ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Canine endothelial antibody
Jackie, Dako's Factor VIII (von Willebrand Factor), cat. #A0082 works for me on canine tissue (and cat, pig, cow, horse, sheep, chicken, mouse, etc.). I use Proteinase K enzyme digestion pretreatment for 5' at room temperature; primary rabbit Ab incubation 30', EnVision+/HRP anti-Rabbit 30' (with 4% normal dog serum added per volume for blocking), AEC 10'. Hope this helps, Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: Jackie M O'Connor Jackie.O'con...@abbott.com To: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Sent: Wednesday, October 28, 2009 3:35 PM Subject: [Histonet] Canine endothelial antibody A week or so ago I thought I saw a post about an antibody that works for canine endothelial cells, but I can't find it in the archives - was I just dreaming, because I need this so bad? I've tried Factor VIII and a CD34 on FFPE, but with poor results. Any help would be most appreciated. Thanks, Jackie O' ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AAVLD Histo sample block slide retention policy
For veterinary diagnostic lab personnel: We're having a difference of opinion here over how long our Histo slides need to be retained. It was my understanding that all paraffin blocks and stained slides need to be retained for 7 years. Others here believe that as long as the blocks are retained, it's not necessary to retain the stained slides past a year or two. Storage space might be a problem in the near future. I've looked through the AAVLD Essential Requirements, but cannot find a definitive answer to this question. Can someone help me out with the correct answer? We're writing a sample storage SOP for each lab section today, and I'd like to get an answer as soon as possible. Thanks in advance, Jan Shivers UMN VDL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
