Re: [Histonet] best paraffin

[John Garratt via Histonet]

Interesting that you are mixing the two.
Is that common practice out there?

John

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On Thu, Mar 10, 2022 at 9:40 AM, Cooper, Brian  wrote:

> Paraplast for processing and Paraplast Xtra for microtomy at our institution.
>
> Thanks,
>
> Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
> Department of Pathology and Laboratory Medicine
> Children's Hospital Los Angeles
> 4650 Sunset Blvd MS#43- Los Angeles, CA 90027
> bcoo...@chla.usc.edu
>
> -Original Message-
> From: Jay Lundgren via Histonet 
> Sent: Thursday, March 10, 2022 9:22 AM
> To: John Garratt ; Rinker, Jeffrey 
> ; histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] best paraffin (EXTERNAL EMAIL)
>
> CAUTION: BE CAREFUL WITH THIS MESSAGE* This email came from outside 
> CHLA. Do not open attachments, click on links, or respond unless you expected 
> this message and recognize the email address: 
> histonet-boun...@lists.utsouthwestern.edu.
>
> Paraplast plus is my personal favorite.
>
> On March 10, 2022, at 11:04 AM, John Garratt via Histonet 
>  wrote:
>
> The paraffins available from lab suppliers (not candle makers) is all of 
> excellent quality these days. There is some variability, but choice of 
> paraffin usually comes down to personal preference.
> If you are having problems cutting it may well be related to other factors 
> like fixation and processing, and of course, the microtomy.
> What are the issues you are encountering?
>
> John
> http://secure-web.cisco.com/1071-nF34Jc0Huepy-CD2D7i4jVe0u3CQxXT-sfZxfJTPdesE2Cm8FUb5nKm9zlp5LAeGb2S-mRT5t0YNkJXRP9yxFC6eIbZWkvU2xGhG7zo9bs6HRYe3J3_UeSdo0VQTrph5P75aQOH1s58spSuO-R3u4LJK8DTNJuJq_j_OtDg2dWHytU5gKPuhKt-OY9w6NdjLPdy_fwi19pr6qF3YFMgPjoLMkuKN_5Xx2Eql8JtaJYuRKcyEDRScQsFXnwW9me2ojfswJ7R5PEGkbd-xjFKSxPuf8S9BimLtV6LbCgcnEhGxaKhr_PQX-UQjP4NUeHoJBE-ATPlQe-a5Im_F4mZt092VRWpnwASWIXe8ob5w6_695blw4gK0jAPU7PyEGtfhIhftxwnB6u-Bfc818ugk8ZBulD3LVuGYoXwjUo2B3s-1MnYxRvVbWseO_ulPMS29Byi2oEe5SRg8lHMc_Q/http%3A%2F%2Fwww.cpqa.ca
>
> Sent from ProtonMail for iOS
>
> On Thu, Mar 10, 2022 at 7:21 AM, Rinker,Jeffrey via Histonet 
>  wrote:
>
>> I was wondering what people think is the best paraffin to use. I find it 
>> hard to get a good read on what is best for our lab. We do around 60-100 
>> blocks a day and do a bx and surgical run overnight. I think that we are 
>> having problems with cutting because we are not optimized and i am trying to 
>> fix that.
>> --
>> - Confidentiality Notice: This e-mail message, including any
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Re: [Histonet] best paraffin

[John Garratt via Histonet]

The paraffins available from lab suppliers (not candle makers) is all of 
excellent quality these days. There is some variability, but choice of paraffin 
usually comes down to personal preference.
If you are having problems cutting it may well be related to other factors like 
fixation and processing, and of course, the microtomy.
What are the issues you are encountering?

John
www.cpqa.ca

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On Thu, Mar 10, 2022 at 7:21 AM, Rinker,Jeffrey via Histonet 
 wrote:

> I was wondering what people think is the best paraffin to use. I find it hard 
> to get a good read on what is best for our lab. We do around 60-100 blocks a 
> day and do a bx and surgical run overnight. I think that we are having 
> problems with cutting because we are not optimized and i am trying to fix 
> that.
> ---
> Confidentiality Notice: This e-mail message, including any attachments,
> is for the sole use of the intended recipient(s) and may contain
> privileged and confidential information. Any unauthorized review, use,
> disclosure or distribution is prohibited. If you are not the intended
> recipient, please contact the sender by reply e-mail and destroy
> all copies of the original message.
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Re: [Histonet] formalin in OR

[John Garratt via Histonet]

I would not normally promote a product but I have seen the TissueSafe and 
SealSafe (Milestone) in use at a couple of hospitals for formalin management in 
the OR and in the lab and was most impressed.

John

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On Tue, Mar 8, 2022 at 9:54 AM, Nancy Schmitt via Histonet 
 wrote:

> Hello-
> I would appreciate input on how you are getting formalin on to the specimens:
>
> * Is it done in OR
> * Are specimens brough to pathology and add formalin there
> * If so - does lab or OR add the formalin
> * Other?
> Are you Joint Commission?
> Thank you!
> Nancy Schmitt MLT, HT(ASCP)
> Pathology Support Services
> Dubuque, IA
>
> Confidentiality Notice:
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> is intended for the sole use of the intended recipient(s). It may contain 
> information that is privileged and confidential. Any unauthorized review, 
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> recipient, please delete this message, and reply to the sender regarding the 
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Re: [Histonet] FW: Biopsy processing

[John Garratt via Histonet]

We (CPQA) ran our first EQA to included a review of tissue processing and 
staining of various tissue and the report might be of interest to you and 
others. Contact me directly if you, or anybody else, would like a copy of the 
report.

John

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On Tue, Oct 5, 2021 at 9:30 AM, Hannen, Valerie via Histonet 
 wrote:

> From: Hannen, Valerie
> Sent: Monday, October 04, 2021 2:54 PM
> To: 'histo...@list.utsouthwestern.edu'
> Subject: FW: Biopsy processing
>
> From: Hannen, Valerie
> Sent: Monday, October 4, 2021 11:03 AM
> To: 'histo...@list.utsouthwestern.edu' 
> Subject: Biopsy processing
>
> Good Morning All... My Pathologist is wondering if there is any benefit to 
> having a separate processing run just for small biopsies? He said that what 
> is seeing now, he has no issue with, the sections are just as good as all the 
> other tissue sections. He just asked for me to get the consensus of the group.
>
> Thank You in advance!!
>
> Valerie A. Hannen,MLT(ASCP),HTL,SU(FL)
> Histology Section Chief
> Parrish Medical Center
> 951 N. Washington Avenue
> Titusville, Florida 32796
> P: 321-268-6333 Ext. 7506
> F: 321-268-6149
> valerie.han...@parrishmed.com
> www.parrishmed.com
>
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Re: [Histonet] release of body parts

[John Garratt via Histonet]

I suggest that path labs start a discussion with Risk Management team and 
lawyers to get advice on the tease of tissues to patients.
The uterus in the landfill or the gallbladder at school “show and tell” will be 
sure to get your legal department on edge and the lab’s name in the local paper.
When everybody is stretched to the limit to provide pathology should you also 
be providing a souvenir service when there is a perfectly good gift shop in the 
hospital?
Having a process in place, like using a funeral home with a lab fee attached 
tends to sort out those who just want something to shows their pals at the 
coffee shop.

John

John

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On Wed, Aug 18, 2021 at 4:44 PM, Jay Lundgren via Histonet 
 wrote:

> It's all fun and games until someone finds a uterus in a landfill.
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Re: [Histonet] "cooked" biopsy

[John Garratt via Histonet]

Thank you for the insights into recycling. Our surveys are based on labs 
achieving a quality of staining and processing that ensures accurate clinical 
interpretation and labs are generally doing an excellent job regardless of the 
reagents used which is a tribute to their QC.
It would be great to see more standardization in histotechnology with the 
increased reliance on anatomic pathology to guide therapy, but I suspect that 
is a pipe dream.
Here is an article that may be of interest

https://link.springer.com/article/10.1007/s00428-020-02960-z

John

On Fri, Jul 9, 2021 at 7:18 PM, Jamie Watson  
wrote:

> Hello everyone,
>
> Tony is correct that generally recycled xylene is purer than what you buy. I 
> have been using recycled xylene since the early 80s from 4 different types of 
> recyclers.
>
> The best practice is to have a small amount of new xylene constantly coming 
> into the recycling stream. We always use purchased xylene on our H stainer 
> to achieve this. Also about every 1-1.5 years replace all the xylene in the 
> recycling stream.
>
> Alcohol recycling is too labor intensive and can be difficult to get the the 
> percentage correct.
>
> I have never used recycled formalin.
>
> Jamie
>
> On July 9, 2021 6:13:13 PM "Tony Henwood (SCHN) via Histonet" 
>  wrote:
>
>> Hi John,
>>
>> There is anecdotal evidence that recycled xylene is of a better quality than 
>> the original purchased product. We have found it to be so. No processing nor 
>> staining problems unless there has been a recycling issue (as shown by the 
>> CBG xylene purity test).
>>
>> I suppose we have to access each reagent that is recycled and determine the 
>> risk of using it. For example, unless care is taken, recycling alcohol can 
>> be an issue (eg xylene contamination). We do not recycle alcohol for this 
>> reason.
>>
>> "When we are continuously challenged by pre-analytic variables in 
>> histotechnology why do labs continue to use recycled reagents? "
>>
>> From your survey, has re-cycling xylene been found to be a major, or minor 
>> issue?
>>
>> We definitely need evidence that re-cycling is a risk.
>>
>> Regards
>> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
>> Principal Scientist, the Children’s Hospital at Westmead
>> Adjunct Fellow, School of Medicine, University of Western Sydney
>> Tel: 612 9845 3306
>> Fax: 612 9845 3318
>> Pathology Department
>> the children's hospital at westmead
>> Cnr Hawkesbury Road and Hainsworth Street, Westmead
>> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>> 
>> From: John Garratt 
>> Sent: Saturday, 10 July 2021 05:31
>> To: Paula; Tony Henwood (SCHN); 'Erick Rodriguez'
>> Cc: histonet@lists.utsouthwestern.edu
>> Subject: Re: [Histonet] "cooked" biopsy
>>
>> Interesting discussion.
>> At CPQA we recently started a H EQA program that includes fixation and 
>> processing in the feedback to participants and in a recent mini-survey I 
>> found that a third of labs use recycled product somewhere in the 
>> pre-analytic phase.
>> When we are continuously challenged by pre-analytic variables in 
>> histotechnology why do labs continue to use recycled reagents?
>>
>> John
>>
>> On Thu, Jul 8, 2021 at 5:59 AM, Paula via Histonet 
>> mailto:histonet@lists.utsouthwestern.edu>>
>>  wrote:
>> Hi Tony,
>> Thank you for the procedure. I do send out the recycled xylene to have its 
>> analysis done by an outside company every month, and I'll put into place 
>> this testing procedure that you outlined below in place ase well.
>> Paula
>>
>> -Original Message-
>> From: Tony Henwood (SCHN) [mailto:tony.henw...@health.nsw.gov.au]
>> Sent: Wednesday, July 07, 2021 1:25 PM
>> To: 'Erick Rodriguez'; Paula
>> Cc: histonet@lists.utsouthwestern.edu
>> Subject: Re: [Histonet] "cooked" biopsy
>>
>> Hi Paula,
>>
>> We can check the purity of the xylene quite easily:
>>
>> Xylene Purity Test Procedure
>>
>> Note: The recommended and most accurate method of determining the purity of 
>> the recycled xylene is by doing a Gas Chromatography analysis. The following 
>> method can be used to obtain an acceptable confidence level in the purity of 
>> the recycled xylene (CBG Biotech).
>>
>> Testing Procedure
>>
>> 1. To a clean, dry 100 ml mixing cylinder graduate, add sufficient recovered 
>> xylene so that the bottom of the meniscus is aligned with the top edge of 
>> the 85 ml mark on the graduate.
>>
>> 2. Add water to the graduate until the bottom of the meniscus aligns with 
>> the top edge of the 100 ml mark on the graduate. At this point, 15 ml of 
>> water will have been added to 85 ml of recovered xylene.
>>
>> 3. Stopper the graduate and invert the mixture. Allow the mixture to settle, 
>> making sure that all of the water settles to the bottom of the graduate. No 
>> water should remain clinging to the sides of the graduate above the 
>> xylene/water separation point. This separation point should be near the 15 
>> ml level of the 

Re: [Histonet] "cooked" biopsy

[John Garratt via Histonet]

Interesting discussion.
At CPQA we recently started a H EQA program that includes fixation and 
processing in the feedback to participants and in a recent mini-survey I found 
that a third of labs use recycled product somewhere in the pre-analytic phase.
When we are continuously challenged by pre-analytic variables in 
histotechnology why do labs continue to use recycled reagents?

John

On Thu, Jul 8, 2021 at 5:59 AM, Paula via Histonet 
 wrote:

> Hi Tony,
> Thank you for the procedure. I do send out the recycled xylene to have its 
> analysis done by an outside company every month, and I'll put into place this 
> testing procedure that you outlined below in place ase well.
> Paula
>
> -Original Message-
> From: Tony Henwood (SCHN) [mailto:tony.henw...@health.nsw.gov.au]
> Sent: Wednesday, July 07, 2021 1:25 PM
> To: 'Erick Rodriguez'; Paula
> Cc: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] "cooked" biopsy
>
> Hi Paula,
>
> We can check the purity of the xylene quite easily:
>
> Xylene Purity Test Procedure
>
> Note: The recommended and most accurate method of determining the purity of 
> the recycled xylene is by doing a Gas Chromatography analysis. The following 
> method can be used to obtain an acceptable confidence level in the purity of 
> the recycled xylene (CBG Biotech).
>
> Testing Procedure
>
> 1. To a clean, dry 100 ml mixing cylinder graduate, add sufficient recovered 
> xylene so that the bottom of the meniscus is aligned with the top edge of the 
> 85 ml mark on the graduate.
>
> 2. Add water to the graduate until the bottom of the meniscus aligns with the 
> top edge of the 100 ml mark on the graduate. At this point, 15 ml of water 
> will have been added to 85 ml of recovered xylene.
>
> 3. Stopper the graduate and invert the mixture. Allow the mixture to settle, 
> making sure that all of the water settles to the bottom of the graduate. No 
> water should remain clinging to the sides of the graduate above the 
> xylene/water separation point. This separation point should be near the 15 ml 
> level of the graduate. (Note: xylene floats on top of the water).
>
> 4. Carefully inspect and record the point of separation between the water and 
> xylene using the bottom of the meniscus as the separation point.
>
> 5. Subtract 15 ml from the quantity of water indicated in step 5. The 
> remainder plus an additional 0.1 correction factor equals the percentage of 
> recovered xylene impurities.
>
> EXAMPLE:
>
> Xylene/Water separation point is indicated to be 15.5 ml.
> (15.5 - 15) + 0.1 = 0.6% impurities.
> Therefore, the recovered xylene is 99.4% pure.
>
> Regards
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
> Principal Scientist, the Children’s Hospital at Westmead
> Adjunct Fellow, School of Medicine, University of Western Sydney
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> Pathology Department
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
> 
> From: Paula via Histonet 
> Sent: Thursday, 8 July 2021 04:53
> To: 'Erick Rodriguez'
> Cc: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] "cooked" biopsy
>
> Thank you, everyone...
> I looked at my reagents and saw the color pink in the xylene, which tells me 
> that there is water or too much water in it so I changed it.
> We recycle xylene, so I need to get the recycler looked at now.
> Thanks again,
> Paula
>
> -Original Message-
> From: Erick Rodriguez [mailto:rodriguez.er...@icloud.com]
> Sent: Wednesday, July 07, 2021 11:24 AM
> To: Paula
> Subject: Re: [Histonet] "cooked" biopsy
>
> Did you change the processor reagents before running your tissues? Cooked 
> tissue usually means the tissue wasn’t dehydrated properly and the leftover 
> water boiled and fried your tissue. I would double check the alcohols.
>
>> On Jul 7, 2021, at 11:00 AM, Paula via Histonet 
>>  wrote:
>>
>> Hello, good day,
>>
>>
>>
>> Our pathologist is complaining about the tissues today that they are
>> "cooked, burnt, crushed, shrunken" those are the adjectives she is using.
>>
>>
>>
>> Can you tell me the cause? Usually, the work comes out beautiful but today
>> they are not. Nothing has changed on our processing times.
>>
>>
>>
>> What should I investigate?
>>
>>
>>
>> Thank you in advance,
>>
>> Paula
>>
>> Bio-Path Medica Group
>>
>>
>>
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> information. If you are not the intended recipient, please delete it and 
> notify the sender.
>
> Views 

Re: [Histonet] Automated Coverslippers

[John Garratt via Histonet]

Having used tape coverslips for over 20 years previous to moving out of the 
clinical lab I can say that I have rarely had a problem with them. They are 
efficient because they dry so quickly so they can be filed efficiently. 
Important for a Lean operation. The key to success with tape coverslips is to 
use good quality xylene ( not recycled ) and make sure you use enough xylene 
(do not skimp).

John.

On Thu, May 13, 2021 at 7:31 AM, Patpxs via Histonet 
 wrote:

> Happy Friday Eve,
>
> We use the Sakura glas2 cover slipper. We run hundreds of slides through them 
> every day with very few problems.
>
> Any automated coverslipper can have issues. A word of warning- watching the 
> g2 do its thing is hypnotic.
>
> Paula
>
> Sent from my iPhone
>
>> On May 13, 2021, at 6:18 AM, Jennifer Phinney via Histonet 
>>  wrote:
>>
>> Hello Histonetters,
>> My lab currently has a Leica CV5030 that is attached to our ST5010 
>> Autostainer. We bought it way back in 2012 and it is starting to have 
>> progressively more issues working reliably. This is the only one that can be 
>> integrated with our stainer, so we know we'll have to get a standalone unit 
>> and transfer slides to a new rack.
>>
>> What automated coverslipper is everyone using? I know in the past there have 
>> been issues with tape coverslippers popping off of the slides when stored 
>> long term, but has this been fixed with newer units? What is everyone's 
>> experience with them?
>>
>> I got a quote for the Leica Spectra coverslipper, but I am not familiar with 
>> what other companies have available.
>>
>> Thanks all,
>> Jennifer Phinney MS QIHC
>> Histology Laboratory Administrator
>> Kansas State Veterinary Diagnostic Lab
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Re: [Histonet] Filed slides sticking together

[John Garratt via Histonet]

Use tape if you want to have a lean mean operation. Instant filing, no 
sticking, no ovens.

John

On Fri, Apr 16, 2021 at 2:55 PM, Jay Lundgren via Histonet 
 wrote:

> I've seen labs that do this, like 30C for 24 hours, but if you use xylene,
> make sure to put the oven under a hood.
>
> It's a lot easier just to leave them out, in a well ventilated place, for
> 48hours. Buy more slide folders/trays.
>
> I worked one place where the slides were held for 24 hours before being
> given to the paths, because they were concerned about being exposed to
> xylene fumes. But that lab/hospital system had no competition within 100
> miles and didn't care about turn around times.
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Re: [Histonet] Oil Red O

[John Garratt via Histonet]

In Histological and Histochemical Methods in Theory and Practice by John 
Kiernan there is a good method for Oil Red O.

There is definitely an art to a good Oil Red O that is passed down from the 
older experienced tech to the young folks in the lab.

Bancroft’s Theory and Practice of Histological Technique is also a good source 
for staining methods.

John

On Sat, Mar 6, 2021 at 8:55 AM, John Kiernan via Histonet 
 wrote:

> It's in any textbook in the field of histotechnology published since about 
> 1950. This one from Amazon costs less than $8. Every lab should have a shelf 
> of such books.
>
> https://www.amazon.com/Introduction-Histotechnology-Geoffrey-G-Brown/dp/0838543405
> [https://images-na.ssl-images-amazon.com/images/I/41xQTLdptPL.jpg1z]m]
> An introduction to histotechnology: A manual for the student, practicing 
> technologist, and resident-in-pathology: Brown, Geoffrey G: 9780838543405: 
> Amazon.com: 
> Books
> An introduction to histotechnology: A manual for the student, practicing 
> technologist, and resident-in-pathology [Brown, Geoffrey G] on Amazon.com. 
> *FREE* shipping on qualifying offers. An introduction to histotechnology: A 
> manual for the student, practicing technologist, and resident-in-pathology
> www.amazon.com
> John Kiernan
> London, Canada
> = = =
> 
> From: Niihori, Maki - (mniihori) via Histonet 
> 
> Sent: March 5, 2021 4:08 PM
> To: histonet@lists.utsouthwestern.edu 
> Subject: [Histonet] Oil Red O
>
> We would like to stain Right Ventricle (RV) and Lung (both from rat samples) 
> with Oil Red O.
> I appreciate if anybody can share a good protocol/kit information with me.
>
> Thank you,
> Maki
>
> *
>
> Maki Niihori, PhD
>
> Life Sciences North Rm# 402,
>
> Department of Medicine,
>
> The University of Arizona
>
> Phone: 520-626-6092
>
> E-mail: mniih...@arizona.edu
>
> *
>
> ___
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Re: [Histonet] Oil Red O

[John Garratt via Histonet]

What have you fixed the tissue with? Can you cut frozen sections from the fixed 
tissue?
This is useful information to start with.

John

On Fri, Mar 5, 2021 at 1:08 PM, Niihori, Maki - \(mniihori\) via Histonet 
 wrote:

> We would like to stain Right Ventricle (RV) and Lung (both from rat samples) 
> with Oil Red O.
> I appreciate if anybody can share a good protocol/kit information with me.
>
> Thank you,
> Maki
>
> *
>
> Maki Niihori, PhD
>
> Life Sciences North Rm# 402,
>
> Department of Medicine,
>
> The University of Arizona
>
> Phone: 520-626-6092
>
> E-mail: mniih...@arizona.edu
>
> *
>
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Re: [Histonet] Floaters

[John Garratt via Histonet]

Interesting thread. Thanks for kicking it off.

Does anybody have a reference that cites diagnostic errors caused by floaters / 
contamination from other specimens?

John

On Mon, Dec 7, 2020 at 9:15 AM, Joe W. Walker, Jr. via Histonet 
 wrote:

> Don’t shoot the messenger. :)
>
> Joe W. Walker, Jr. MS, SCT(ASCP)
> Anatomical Pathology and Interim Phlebotomy Manager
> Rutland Regional Medical Center
> 160 Allen Street, Rutland, VT 05701
> P 802.747.1790 F 802.747.6525
> joewal...@rrmc.org, www.rrmc.org
>
> -Original Message-
> From: Terri Braud via Histonet 
> Sent: Monday, December 7, 2020 8:42 AM
> To: 'histonet@lists.utsouthwestern.edu' 
> Subject: Re: [Histonet] Floaters
>
> [External Email] This email originated from outside of the organization. 
> Think before you click: Don’t click on links, open attachments or respond to 
> requests for sensitive information if the email looks suspicious or you don’t 
> recognize the sender.
>
> 60% of floaters from the water bath? I find that really hard to believe.
> The Gephardt and Zarbo CAP study from 1996 showed reported the results of a 
> Q-Probes study of 275 laboratories and documented a frequency of 
> contamination of between 0.6% and 2.9%, depending on the study method. Their 
> study demonstrated the rate of extraneous tissue contamination was higher for 
> blocks than for slides and higher in a retrospective review than in a 
> prospective study. So in other words, when people knew they were being 
> studied, they were more careful and the contamination rate went down, but in 
> retrospect, the majority of floaters occurred in the blocks, not the water 
> bath.
>
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> HNL Labs, Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
> Today's Topics:
>
> 1. "Floaters" in surgical or cytology specimens
> (Martha Ward-Pathology)
> 2. Re: "Floaters" in surgical or cytology specimens
> (Joe W. Walker, Jr.)
>
> --
>
> Message: 1
> Date: Fri, 4 Dec 2020 13:55:20 +
> From: Martha Ward-Pathology 
> To: "histonet@lists.utsouthwestern.edu"
> 
> Subject: [Histonet] "Floaters" in surgical or cytology specimens
> Message-ID:
> 
>
> Content-Type: text/plain; charset="us-ascii"
>
> I am posting this question for a colleague in our Cytology department. How 
> often do you see floaters on surgical or cytology specimens? Obviously we 
> would never want to see any type of carryover but is there a standard rate 
> published somewhere that he can reference?
>
> Thanks in advance for your help.
>
> Martha Ward, MT ASCP, QIHC
> Manager, Molecular Diagnostics Lab
> Wake Forest Baptist Medical Center
>
> --
>
> Message: 2
> Date: Fri, 4 Dec 2020 17:41:11 +
> From: "Joe W. Walker, Jr." 
> To: Martha Ward-Pathology 
> Cc: "histonet@lists.utsouthwestern.edu"
> 
> Subject: Re: [Histonet] "Floaters" in surgical or cytology specimens
> Message-ID:
> 
>
> Content-Type: text/plain; charset="utf-8"
>
> https://urldefense.com/v3/__https://academic.oup.com/ajcp/article/136/5/767/1766314__;!!I87qwjxLstg3H_X5!rJ2yq9KcDC2PooORZtJvXi4R8vHOIg5tak39dSSWFLa5SL1M73A18pgYpUvPASA$
>
> "Floaters represent a potential source of diagnostic error and occur in 0.01% 
> to 1.2% of slides. Pick up of floaters from the water bath appears most 
> common (?60%). Floaters in only 1 level and mismatch with the specimen tissue 
> type are clues to the extraneous nature of the floater."
>
> Joe W. Walker, Jr. MS, SCT(ASCP)
> Anatomical Pathology and Interim Phlebotomy Manager Rutland Regional Medical 
> Center
> 160 Allen Street, Rutland, VT 05701
> P 802.747.1790 F 802.747.6525
> joewal...@rrmc.org, http://www.rrmc.org
>
> -Original Message-
> From: Martha Ward-Pathology via Histonet 
> Sent: Friday, December 4, 2020 8:55 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] "Floaters" in surgical or cytology specimens
>
> [External Email] This email originated from outside of the organization. 
> Think before you click: Don?t click on links, open attachments or respond to 
> requests for sensitive information if the email looks suspicious or you don?t 
> recognize the sender.
>
> I am posting this question for a colleague in our Cytology department. How 
> often do you see floaters on surgical or cytology specimens? Obviously we 
> would never want to see any type of carryover but is there a standard rate 
> published somewhere that he can reference?
>
> Thanks in advance for your help.
>
> Martha Ward, MT ASCP, QIHC
> Manager, Molecular Diagnostics Lab
> Wake Forest Baptist Medical Center
> ___
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> Histonet@lists.utsouthwestern.edu
> 

Re: [Histonet] hexamethyleneimine

[John Garratt via Histonet]

It is better known as Hexamine in histo circles. Gomori Hexamine Silver, for 
example, can be used to nicely demonstrate Glomeruli.

John

On Sun, Oct 11, 2020 at 3:45 PM, LEROY H BROWN via Histonet 
 wrote:

> What do you use hexamethyleneimine for in your lab. I found an old bottle
> of this reagent and cannot recall why I have it.
>
> It must be used in making up a stain but I am not remembering what stain.
>
> Anyone know?
>
> Thanks
>
> LeRoy Brown HT(ASCP)HTL
>
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Re: [Histonet] Fume hood filter

[John Garratt via Histonet]

You could purchase activated charcoal and replace the contents in the old filter

John

On Thu, Sep 24, 2020 at 8:57 AM, Hannen, Valerie via Histonet 
 wrote:

> Hi everyone.. hope all are well!!
>
> I have a slight dilemma... I have a "Fume-Gard" (old) fume hood and my 
> current vendor is out and will not be restocking. Is there anyone else out 
> there that is using this fume hood for coverslipping?? The dimensions are as 
> follows: H: 5.8 inches, D: 1.5 inches and W: 11.2.
>
> Can anyone steer me to a vendor who might have such filters with these 
> dimensions?
>
> Thanks !!
>
> Valerie Hannen,MLT(ASCP),HTL,SU (FL)
> Section Chief, Histology
> Parrish Medical Center
> 951 N. Washington Ave.
> Titusville,Florida 32796
> T: (321)268-6333 ext. 7506
> F: (321) 268-6149
> valerie.han...@parrishmed.com
> www.parrishmed.com
>
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Re: [Histonet] Sausage controls

[John Garratt via Histonet]

A control block made of four tissues will cover the majority of IHC stains. Use 
appendix, tonsil, pancreas and liver in the block. It is important to select 
the tissues carefully to ensure they are fixed and processed appropriately. It 
is also important to test and document the tissues before adding them to the 
multi tissue block. If anybody needs more information on the testing and 
building protocol please contact me directly.

John

On Thu, Aug 20, 2020 at 11:12 AM, Sheeder, Christopher via Histonet 
 wrote:

> Here is the article...
> Multitumor "Sausage" Blocks in Immunohistochemistry Simplified Method of 
> Preparation, Practical Uses, and Roles in Quality Assurance
> Rodney T. Miller, M.D., Carol L. Groothuis, MT(ASCP)SI
> American Journal of Clinical Pathology, Volume 96, Issue 2, 1 August 1991, 
> Pages 228-232, https://doi.org/10.1093/ajcp/96.2.228
>
> Christopher Sheeder, HT(ASCP)CMQIHC
> Histology Supervisor | Department of Laboratories
> Seattle Children's Hospital
> 4800 Sand Point Way NE
> Seattle, WA 98105
> Office: 206-987-6259
>
> christopher.shee...@seattlechildrens.org
>
> Seattle Children's - Internal
>
> -Original Message-
> From: Mac Donald, Jennifer 
> Sent: Wednesday, August 19, 2020 10:30 AM
> To: Emily Horst 
> Cc: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Sausage controls
>
> The Journal of Histotechnology had an article regarding this years ago. 
> Perhaps you can find it in the archives. Dr. Hector Battifora was the author.
> Jennifer
>
> -Original Message-
> From: Emily Horst via Histonet 
> Sent: Wednesday, August 19, 2020 10:11 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Sausage controls
>
> EXTERNAL SENDER- Exercise caution with requests, links, and attachments.
>
> Hello all,
>
> For those of you who use a sausage control for IHC/special stains, could you 
> direct me as to process for starting that instead of using individual 
> controls for everything?
>
> Thanks in advance!
>
> Emily
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>
> CONFIDENTIALITY NOTICE: This e-mail, including any attachments, is for the 
> sole use of the intended recipient(s) and may contain confidential and 
> privileged information protected by law. Any unauthorized review, use, 
> disclosure or distribution is prohibited. If you are not the intended 
> recipient, please contact the sender by reply e-mail and destroy all copies 
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Re: [Histonet] Acid Fast

[John Garratt via Histonet]

Hi Ken, have you checked all your reagents for contamination?

John

On Tue, Aug 11, 2020 at 7:34 AM, Ken M via Histonet 
 wrote:

> Hi everyone- We have used a modified Ziehl Neelson stain on some breast 
> tissue and found round shape organisms that are Acid Fast and not rod-like. 
> Is anyone familiar with this before I send them to our Pathologist?
>
> Ken
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Re: [Histonet] process formalin-fixed tissues from animals infected with a virus

[John Garratt via Histonet]

Evaluation of Virus Inactivation by Formaldehyde to Enhance Biosafety of 
Diagnostic Electron Microscopy

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353909/

It is nice to have a reference.

John

On Thu, Aug 6, 2020 at 4:10 AM, Greg Dobbin via Histonet 
 wrote:

> Hi Amy,
> Formalin fixed tissue is no longer infectious...unless you are talking
> about prions (eg scrapie, BSE, etc). So there should otherwise be no
> concerns or additional precautions required.
> Cheers,
> Greg
>
> --
> *Greg Dobbin*
> 1205 Pleasant Grove Rd
> RR#2 York,
> PE C0A 1P0
>
> *Everything in moderation...even moderation itself**!*
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Re: [Histonet] Apple Green Birefringence in Amyliod slides

[John Garratt via Histonet]

Here are a couple of short videos that might be of interest. - John
https://www.bing.com/videos/search?q=amyloid+birefringence&=detail=0663C1FAAFFA319FD8EA0663C1FAAFFA319FD8EA&=VRDGAR=%2Fvideos%2Fsearch%3Fq%3Damyloid%2Bbirefringence%26FORM%3DHDRSC3


https://www.bing.com/videos/search?q=amyloid+birefringence=%2fvideos%2fsearch%3fq%3damyloid%2bbirefringence%26FORM%3dHDRSC3=detail=25C492C77CCC4B969E6625C492C77CCC4B969E66&=VDRVRV

www.cpqa.ca

‐‐‐ Original Message ‐‐‐
On Tuesday, July 28, 2020 11:43 AM, Ken M via Histonet 
 wrote:

> Hi everyone. I was wondering if anyone out there has any experience with 
> diagnosing Amyloid tissue using Congo Red stained Kidney using polarized 
> lenses. Is it common to use polarized light to detect Amyloid deposits? Does 
> the absence of the "apple green birefringence" indicate a problem with the 
> control tissue or the control slides? Should this green bifringence always 
> appear to confirm the diagnosis? I know that the tissue should be cut thicker 
> than normal (we usually cut at 5), but in the future maybe we will cut at 7 
> or 8?
>
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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Re: [Histonet] FNA Procedures

[John Garratt via Histonet]

We published the following article a few years back in Acta Cytologica that 
outlines what we are doing to manage cases without on site evaluation. Stats 
like these help to make your case. Ultimately we found the imaging department 
preferred not to be waiting for lab and they were able to become more 
efficient. I hope this helps - John

Gastrointestinal Endoscopic Ultrasound-Guided Fine-Needle Aspiration Biopsy 
Specimens: Adequate Diagnostic Yield and Accuracy Can Be Achieved without 
On-Site Evaluation

Article in [Acta 
cytologica](https://www.researchgate.net/publication/journal/0001-5547_Acta_cytologica)
 59(4) · September 2015

On Wed, Jul 8, 2020 at 12:26 PM, Amy Self via Histonet 
 wrote:

> Hope everyone is having a wonderful Wednesday...
>
> We (histology) have been doing what seems like tons of FNA procedures 
> (Radiology/EBUS\EUS) here lately as well as BM procedures. With all of the 
> Covid going on around us we are working short staffed and doing the best we 
> can with what we have, but it seems like the physicians are scheduling these 
> procedure's around what works best for them and this seems to be creating 
> some issues here in pathology, especially trying to work between two 
> hospitals because they are scheduling these procedure's when we don't seem to 
> have staff available or they will call and say we have an add-on at the last 
> minute and will need pathology when in fact they end not needing our 
> services. Oh yea and we do not have an on-site cytotech so histology techs 
> are the ones that are helping assist with FNA's.
>
> I was wondering what other facilities are doing as far as working with the 
> other departments with these FNA procedures after hours. Do you have cut-off 
> times, or have them place the aspirated material in formalin for cell block, 
> train the nursing staff to make smears and hope they are good samples or do 
> you just accommodate and go to the procedure if needed?
>
> I think that it's time for a SOP to be put in place.
>
> Thanks,
> Amy Self
> Senior Histology Technologist
> Tidelands Georgetown Memorial Hospital
> 606 Black River Road
> Georgetown, SC 29440
> Office: (843) 520-8711
> as...@tidelandshealth.org
> Our mission: We help people live better lives through better health.
>
> NOTE:
> The information contained in this message may be privileged, confidential and 
> protected from disclosure. If the reader of this message is not the intended 
> recipient, or an employee or agent responsible for delivering this message to 
> the intended recipient, you are hereby notified that any dissemination, 
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Re: [Histonet] Xylene recycle or purchase fresh

[John Garratt via Histonet]

You may wish to consider consulting with your local fire officer to find out 
what requirements are for installing a recycling unit in your facility. You may 
not even be allowed to place one.
Also, consider the fumes from recycling xylene and if you want your expensive 
lab labour to be taken off the bench to process xylene.

John


www.cpqa.ca

‐‐‐ Original Message ‐‐‐
On Tuesday, June 23, 2020 5:52 PM, Eddie Martin via Histonet 
 wrote:

> Hi Anne,
>
> If your xylene consumption is small, the footprint needed to recycle xylene 
> in house and associated cost would be more expensive than buying xylene and 
> shipping out the waste.
>
> V/r,
> Eddie Martin
>
> Sent from my iPhone
>
> > On Jun 23, 2020, at 10:39 AM, Anne Murvosh amurv...@advancederm.net wrote:
> > I'm looking to switch from a disposal pick-up to recycling of Xylene. Can 
> > anyone tell me some companies that offer a recycling unit. The catch is we 
> > don't have very much so I need something for a small lab. Thanks Anne
>
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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Re: [Histonet] p53

[John Garratt via Histonet]

http://cpqa.ca/assessments/Run%2095%20p53%20Summary%20(final).pdf

The above is an EQA report from CPQA /CIQC for p53 which gives specific 
protocols. In addition, if you go to the end of the report, there are the 
reporting guidelines for p53.
EQA reports are also available for other antibodies when you go to our web site.


John


www.cpqa.ca

‐‐‐ Original Message ‐‐‐
On Monday, May 4, 2020 7:40 AM, Jacque Sagasser via Histonet 
 wrote:

> Hello,
>
> Our lab has been using this down time to validate a few antibodies. So far we 
> have validated Ki67, AE 1/3, CK20, and CDX-2. I am in the process of 
> validating the P53 (DO-7) antibody, but am having trouble producing positive 
> expression. I am using the same colon adenocarcinoma tissue I used to 
> validate all of the antibodies that I previously mentioned. We are using the 
> Ventana Benchmark XT along with the OptiView DAB. The package insert says to 
> use colon adenocarcinoma for a control tissue, and the iView DAB. In the 
> past, I have worked at a facility that ran it using the UltraView DAB so I 
> don't think using the OptiView DAB is the issue. My Roche representative said 
> he has heard of customers having to use a different control for the P53. Do 
> you have any suggestions for controls or tweaking the protocol for this 
> antibody given the parameters we are using? Any advice would be appreciated.
>
> Jacque R. Sagasser, HT (ASCP)cm
> Gandhi GI Pathology, LLC
> Kettering OH
>
> Histonet mailing list
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Re: [Histonet] CAP proficiency testing

[John Garratt via Histonet]

The Canadian Pathology Quality Assurance programme runs IHC and FISH EQA 
internationally. We have performed PD-L1 EQA previously and we will be running 
a challenge January 2021. Previous EQA reports are available on line. Our 
website is www.cpqa.ca.
We work with regularity authorities to provide compliance information when 
requested by the participant.

John

On Thu, Apr 16, 2020 at 10:38 AM, Charles Riley via Histonet 
 wrote:

> Does anyone use another vendor besides CAP to perform their PT
> requirements?
>
> If so, who do you use and how do you record it for inspection purposes?
>
> I am looking for a PD-L1 PT specifically but will take any other options as
> well as we are trying to help our budget.
>
> --
>
> Charles Riley BS HT, HTL(ASCP)CM
>
> Histopathology Coordinator/ Mohs
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Re: [Histonet] Histonet Digest, Vol 197, Issue 8

[John Garratt via Histonet]

Good morning, please take a look at one of the EQA reports (example in the link 
below) for MMR from the Canadian Pathology Quality Assurance programme. 
(www.cpqa.ca) .
You cannot say MMR is positive or negative since it is confusing but say if 
there is Expression or Absence of Expression of a MMR protein. Fortunately 
tissues come with their own internal "positive" and "negative" controls which 
you can utilise. You could design a verification program around the presence or 
absence of a particular MMR protein should you wish.

http://cpqa.ca/assessments/Run%2096%20MMR%20Summary.pdf

Should you wish to receive our technically useful EQA reports in your mailbox 
email subscripti...@cpqa.ca putting SUBSCRIBE in the subject box.

John


www.cpqa.ca

‐‐‐ Original Message ‐‐‐
On Tuesday, April 14, 2020 6:54 AM, Dionee Neamand via Histonet 
 wrote:

> Hello. I am looking for help with IHC staining. Does anyone have good 
> negative control tissue for MMR stains? We need a minimum of 10 negative 
> results per IHC stain for our validation. We have yet to find any. Any 
> suggestions would be helpful.
> Thanks,Dee
> Dionee Neamand, HT (ASCP)Lead TechEvangelical Community HospitalLewisburg  PA 
>  17837
>
> -Original Message-
> To: histonet@lists.utsouthwestern.edu
> Sent: Thu, Apr 9, 2020 1:00 pm
> Subject: Histonet Digest, Vol 197, Issue 8
>
> Send Histonet mailing list submissions to
>     histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
>     http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
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>     histonet-ow...@lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
> Today's Topics:
>
>   1. Question concerning H-Pylori Staining (Ken M)
>   2. Is there anything I can do for you? (Pam Barker)
>   3. Competency questionnaires (Charles Riley)
>
>
> --
>
> Message: 1
> Date: Wed, 8 Apr 2020 19:50:09 +
> From: Ken M kdea...@hotmail.com
> To: Histo List histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Question concerning H-Pylori Staining
> Message-ID:
>     
> sn6pr06mb43343f3f0c62cba60bde54c8ad...@sn6pr06mb4334.namprd06.prod.outlook.com
>
>    
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hi everyone!  We have a question about staining for H-Pylori Using Quick 
> Stain (Periodic acid 1%, Alician Yellow, Sodium Metabisulfate,Toluidine Blue 
> stock, Sodium Hydroxide) we notice what clearly  looks like the H-Pylori 
> purple stained clusters, but after dehydration in 100% alcohol the purple 
> clusters seem to disappear. Should we just dehydrate using a slide oven 
> instead of the alcohol? For how long at what temp? Could the alcohol be 
> affecting the purple color making it too light to see?
>
> Michelle
>
>
> ---
>
> Message: 2
> Date: Thu, 9 Apr 2020 11:15:47 -0400
> From: "Pam Barker" rel...@earthlink.net
> To: "Histopeeps Histonet" histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Is there anything I can do for you?
> Message-ID: 008901d60e81$bf898880$3e9c9980$@earthlink.net
>
> 

Re: [Histonet] BRAF testing

[John Garratt via Histonet]

CPQA have performed at least six B-Raf quality assurance challenges over the 
last few years using TMAs made of 40 tissue cores with known mutational status. 
Participating labs
have consistently done well and their protocols are available within the 
reports. In addition to the results there are some photographs for reference. 
Go to http://cpqa.ca/assessments/Run%20101%20BRAFV600E%20Summary.pdf to see the 
B-Raf report from last year.

Should you be interested in a clinically based EQA programme for IHC or just 
wish to know more about our organisation go to www.cpqa.ca
Also, I do have a detailed power point on B-Raf validation, which includes 
other tissue types and cell lines. Please contact me directly for that if 
interested. Since we have validated cell lines you may wish to consider using 
them for working up the antibody. Go to http://www.histocyte.com/ and 
https://www.statlab.com/truq for cell line information.


john.garr...@cpqa.ca



‐‐‐ Original Message ‐‐‐
On Friday, March 27, 2020 7:05 AM, Cartun, Richard via Histonet 
 wrote:

> This is a very difficult target to detect. I was told recently by a vendor 
> that one needs to use prolonged, very aggressive antigen retrieval (pressure 
> cooker with high pH buffer) to get consistent results. Also, the type of 
> cancer may influence your success (colon vs. thyroid vs. melanoma). I would 
> be interested in knowing what others' experiences are.
>
> Richard
>
> Richard W. Cartun, MS, PhD
> Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
> Proteomics Laboratory Director, Biospecimen Collection Programs Assistant 
> Director, Anatomic Pathology Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 972-1596 (Office)
> (860) 545-2204 (Fax)
> richard.car...@hhchealth.org
>
> -Original Message-
> From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Friday, March 27, 2020 8:07 AM
> To: Histo List
> Subject: [Histonet] BRAF testing
>
> This is an email from an external source. USE CAUTION opening attachments or 
> links from unknown senders.
>
> Can anyone send me a block that can be used to validate BRAF V600E antibody. 
> I have tried several cases that should work but our pathologists don't think 
> it is working correctly. I just need a small core sample that is known to be 
> positive. Any help would greatly be appreciated
>
> ---
>
> Charles Riley BS HT, HTL(ASCP)CM
>
> Histopathology Coordinator/ Mohs
>
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> https://urldefense.com/v3/http://lists.utsouthwestern.edu/mailman/listinfo/histonet;!!KCs9X-8!Oq9k97gGgRatvdlIoqX-QkrcMRMS19Iuxle4pZY1FqKfIdEBOVk2YOJ3yVO0qAn3MBg$
>
> Reminder: This e-mail and any attachments are subject to the current HHC 
> email retention policies. Please save or store appropriately in accordance 
> with policy.
>
> This e-mail message, including any attachments, is for the sole use of the 
> intended recipient(s) and may contain confidential and privileged 
> information. Any unauthorized review, use, disclosure, or distribution is 
> prohibited. If you are not the intended recipient, or an employee or agent 
> responsible for delivering the message to the intended recipient, please 
> contact the sender by reply e-mail and destroy all copies of the original 
> message, including any attachments.
>
> Histonet mailing list
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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Re: [Histonet] Incomplete cross sections of all tissue in blocks

[John Garratt via Histonet]

I suggest that each histotech is responsible for the blocks they cut and they 
cut the deepers on the their own blocks when they are requested. With feedback 
on the reason for the deeper from the pathologists they (the techs) will become 
more confident and learn how deep to cut.

John

On Tue, Mar 3, 2020 at 7:31 AM, Amy Self via Histonet 
 wrote:

> Good Morning HistoNetters,
>
> I am reaching out to the histonet in hopes to get some suggestions from you 
> on how to handle incomplete cross-sections of tissue in blocks. We are a 
> small lab so this has not been an issue in the past but now that we are 
> growing and our staff has increased I am getting feed-back from pathologist 
> that the sections of tissue are not complete. They are asking for too many 
> deepers that possibly could be avoided if it was cut deep enough to begin 
> with. I have been given some managerial type duties – which I don’t like 
> cause I know nothing about managing people and I need to approach this but I 
> need to approach this issue correctly. Do you have the histotech compare 
> his/her cut slides to the block to make sure that a complete cross-section is 
> obtained and is this documented somehow? Any and all suggestions I need.
>
> Thanks in advance for your help and as always you all rock.. 
>
> Amy Self
> Histology Lab Senior Tech
> Lab
> Tidelands Georgetown Memorial Hospital
> 606 Black River Road
> Georgetown, SC 29440
> (843) 520-8711
> as...@tidelandshealth.org
> Our mission: We help people live better lives through better health.
>
> NOTE:
> The information contained in this message may be privileged, confidential and 
> protected from disclosure. If the reader of this message is not the intended 
> recipient, or an employee or agent responsible for delivering this message to 
> the intended recipient, you are hereby notified that any dissemination, 
> distribution or copying of this communication is strictly prohibited. If you 
> have received this communication in error, please notify us immediately by 
> replying to this message and deleting it from your computer.
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Re: [Histonet] External alarm for Tissue-Tek VIP E300

[John Garratt via Histonet]

There are wiring ports on the E3000 for an external alarm. You have to decide 
where the alarm can be monitored 24/7 at your site. If you mean a wireless 
monitoring system, and these are systems, take a look at Thermo. 
https://www.thermofisher.com/ca/en/home/life-science/lab-equipment/wireless-monitoring-systems.html

John


www.cpqa.ca

‐‐‐ Original Message ‐‐‐
On Thursday, February 27, 2020 12:21 PM, Katie via Histonet 
 wrote:

> I am looking to get an external alarm for our tissue processor. Any
> suggestions? Thanks so much!
>
> Katie Riley-Hamilton
> Technical Supervisor of Dermatopathology
>
> Puyallup Dermatology Clinic
>
> ka...@puyallupderm.com
>
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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Re: [Histonet] IHC troubleshooting

[John Garratt via Histonet]

Do you float and pick up your sections from a water bath of distilled water 
with no adhesive added? If not, doing that will help.

John

On Wed, Feb 26, 2020 at 6:41 AM, Charles Riley via Histonet 
 wrote:

> Our pathologists are complaining that chunks of the dermis are missing from
> IHC slides yet the entire section is present prior to staining.
>
> Does anyone have any ideas what could cause the tissue to not adhere to the
> slides throughout the staining process? We use the Leica Bond stainers.
>
> --
>
> Charles Riley BS HT, HTL(ASCP)CM
>
> Histopathology Coordinator/ Mohs
> ___
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Re: [Histonet] histologists assisting with transplants

[John Garratt via Histonet]

Without knowing the specifics it could be simply to assist at frozen section to 
confirm the eligibility of the donor organ.Dig deeper.


www.cpqa.ca

‐‐‐ Original Message ‐‐‐
On Thursday, February 13, 2020 4:42 AM, Eileen Akemi Allison via Histonet 
 wrote:

> Good morning histoland!
> Just curious, this is definitely a 1st for me. Received a call this morning 
> from my histotech who is on call this week that she was called in at 3:30 AM 
> to assist one our pathologists with a liver transplant. In all my years 
> working in this field, histologists have never had anything to do with 
> transplants. Have any of you out there had anything to do with transplants?
>
> Akemi Allison, BS, HTL
> Histology Supervisor
> UMC El Paso, TX
>
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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Re: [Histonet] Need a procedure

[John Garratt via Histonet]

Good point regarding the molecular and another good reason to move away from 
plasma. Plus taking plasma, even outdated plasma, is fraught with ethical and 
regularity problems which are best to avoid now that ever milliliter of blood 
product needs to be accounted for.


www.cpqa.ca

‐‐‐ Original Message ‐‐‐
On Thursday, January 23, 2020 3:41 PM, Garrey Faller  wrote:

> Great helpful information.
> We use plasma /thrombin.
> It’s easy to use, and we have plenty of access to it from our blood bank.
>
> However, we are looking to switch to Gel.
>
> Why?
>
> 1.  If you don’t keep an eye on your plasma, at some point you could see 
> fungi in your specimen with a chance you might think it’s real and not a 
> contaminant. That’s a problem. Don’t keep it for too long in the 
> refrigerator. It’s a great culture medium for fungi.
> You could freeze small aliquots I guess.
>
> 2.  Molecular testing on cell blocks:
> With the increasing use of molecular testing on cell blocks, plasma 
> thrombin method poses a problem. The plasma introduces other peoples DNA into 
> the patients sample.Think about that.
>
> Didn’t know about the heat issue with Gel. Important to know. Thanks !
>
> Garrey
>
> Sent from my iPhone
>
>
> > On Jan 23, 2020, at 6:12 PM, Tony Henwood (SCHN) via Histonet 
> > histonet@lists.utsouthwestern.edu wrote:
> > Hi all,
> > Be careful of using cell block matrix that requires heat to solubilise the 
> > matrix (eg agar or other commercial matrixes like Histogel).
> > Adding a heated matrix to unfixed, or even formalin fixed, material can 
> > denature some antigens (eg CEA) resulting in a false negative IPX.
> > Unfortunately the importance of heat as a pre-analytical factor in 
> > immunohistochemistry is often not appreciated.
> > Regards
> > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
> > Principal Scientist, the Children’s Hospital at Westmead
> > Adjunct Fellow, School of Medicine, University of Western Sydney
> > Tel: 612 9845 3306
> > Fax: 612 9845 3318
> > Pathology Department
> > the children's hospital at westmead
> > Cnr Hawkesbury Road and Hainsworth Street, Westmead
> > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
> > -Original Message-
> > From: John Garratt via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> > Sent: Friday, 24 January 2020 8:21 AM
> > To: Muhammad Azam ajj...@gmail.com
> > Cc: histonet@lists.utsouthwestern.edu
> > Subject: Re: [Histonet] Need a procedure
> > http://www.avantec.fr/content/dam/tfs/SDG/APD/APD Documents/Product Manuals 
> > & Specifications/Histology Equipment and Supplies/Embedding Cassettes and 
> > Molds/92957066-Richard-Allan-Scientific-HistoGel-Instructions-for-Use.pdf
> > The above link will help.
> > www.cpqa.ca
> > ‐‐‐ Original Message ‐‐‐
> >
> > > On Thursday, January 23, 2020 12:13 PM, Muhammad Azam ajj...@gmail.com 
> > > wrote:
> > > Anybody has validated procedure for histogel
> > > Sent from my iPhone
> > >
> > > > > On Jan 23, 2020, at 1:07 PM, John Garratt via Histonet 
> > > > > histonet@lists.utsouthwestern.edu wrote:
> > > > > Hi Terri, I suggest you use Histogel for block preparation. It works 
> > > > > exceptionally well, it is good for IHC and does not have the pitfalls 
> > > > > of plasma/thrombin.
> > > > > Plasma/thrombin does work well for cell blocks but you will have to 
> > > > > consider an ethical and safe source for your plasma.
> > > > > The instructions for using Histogel are in the package insert though 
> > > > > I have one comment. Be careful how you warm the Histogel and use a 
> > > > > heat block. Do NOT use a microwave since there is a tendency to 
> > > > > overheat the gel and you will end up with poor quality IHC.
> > > > > John
> > > > > www.cpqa.ca
> > > > > ‐‐‐ Original Message ‐‐‐
> > > >
> > > > > On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet 
> > > > > histonet@lists.utsouthwestern.edu wrote:
> > > > > Hi fellow Histonetters - I'm in need of some help, please
> > > > > Background - We currently use agar to capture our scant cell
> > > > > blocks for processing. I am unfamiliar with the Plasma/Thrombin 
> > > > > method of cell block preparation and am interested in comparing it to 
> > > > > our current method Request - Could you please send me your procedures 
&

Re: [Histonet] Need a procedure

[John Garratt via Histonet]

Hi Terri, I suggest you use Histogel for block preparation. It works 
exceptionally well, it is good for IHC and does not have the pitfalls of 
plasma/thrombin.
Plasma/thrombin does work well for cell blocks but you will have to consider an 
ethical and safe source for your plasma.
The instructions for using Histogel are in the package insert though I have one 
comment. Be careful how you warm the Histogel and use a heat block. Do NOT use 
a microwave since there is a tendency to overheat the gel and you will end up 
with poor quality IHC.

John


www.cpqa.ca

‐‐‐ Original Message ‐‐‐
On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet 
 wrote:

> Hi fellow Histonetters - I'm in need of some help, please
> Background - We currently use agar to capture our scant cell blocks for 
> processing. I am unfamiliar with the Plasma/Thrombin method of cell block 
> preparation and am interested in comparing it to our current method
> Request - Could you please send me your procedures for this method, 
> specifically where you purchase your plasma and thrombin and what species are 
> used?
> Thanks in advance. Histotechs rock!
>
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
>
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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Re: [Histonet] Skin Sample with Tattoo - need to remove for diagnosis

[John Garratt via Histonet]

It is a pigment (ie carbon) and not like a soluble ink, so good luck with that.
You could consult your histotech text book on exogenous pigment removal and 
give the recipe a go. I will certainly be interested in other histonet replies.

John



www.cpqa.ca

‐‐‐ Original Message ‐‐‐
On Friday, January 17, 2020 8:16 AM, Donna Emge via Histonet 
 wrote:

> Hello fellow histonetters,
>
> We received a skin sample that has ink from a tattoo - the sample if from
> tattooed skin. One of our pathologists would like us to see if we can get
> rid of the tattoo ink from the sections before H staining. Does anyone
> out there know how to do this?
>
> Thank you,
> Donna Emge
> Anatomic Pathology Manager
> Mercy Hospital and Medical Center, Chicago
>
> -
>
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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Re: [Histonet] Need a Slide Scanner

[John Garratt via Histonet]

I suggest contacting Hamamatsu in the US.

dariyaku...@hamamatsu.com

John

Sent from ProtonMail Mobile

On Thu, Jan 9, 2020 at 2:28 PM, Glover, Kimberly via Histonet 
 wrote:

> Hi Tim,
>
> Thank you for your help with my search. It sounds like I will need to use a 
> high resolution camera if there are no options for a combined system or 
> either select 2 systems.
>
> Kimberly
> -Original Message-
> From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu]
> Sent: Thursday, January 09, 2020 5:12 PM
> To: Glover, Kimberly
> Subject: RE: Need a Slide Scanner
>
> No, it won't accommodate that. I'm not sure any will. It would take a long 
> time to scan a slide at that magnification. You may be better off with 
> individual pictures for that.
>
> Tim Morken
> Supervisor, Electron Microscopy/Neuromuscular Special Studies
> Department of Pathology
> UC San Francisco Medical Center
>
> -Original Message-
> From: Glover, Kimberly 
> Sent: Thursday, January 09, 2020 12:40 PM
> To: Morken, Timothy 
> Cc: hiso...@list.utsouthwestern.edu
> Subject: RE: Need a Slide Scanner
>
> Hi Tim,
>
> Will the Mikroscan be good for oil immersion slides also?
>
> Kimberly
>
> -Original Message-
> From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu]
> Sent: Thursday, January 09, 2020 2:32 PM
> To: Glover, Kimberly
> Subject: RE: Need a Slide Scanner
>
> If you only do small batches the Mikroscan is great. The Philips is very 
> expensive and designed for large batch high-throughput.
>
> You only need a scanner where you have the slides to scan. The images go on a 
> server for viewing. We have one at Each gross room because the slides might 
> be viewed by pathologists anywhere in our 3 facilities.
>
> Tim Morken
> Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
> Pathology UC San Francisco Medical Center
>
> -Original Message-
> From: Glover, Kimberly 
> Sent: Thursday, January 09, 2020 11:23 AM
> To: Morken, Timothy 
> Subject: RE: Need a Slide Scanner
>
> Hi Tim,
>
> We will send slides out throughout the day for remote diagnosis. Is the 
> Mikroscan L5 better for this? Do we need to have a scanner at both locations?
>
> Thanks,
> Kimberly
>
> -Original Message-
> From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu]
> Sent: Thursday, January 09, 2020 2:14 PM
> To: Glover, Kimberly
> Subject: RE: Need a Slide Scanner
>
> Kimberly, all at once, or staggered throughout the day?
>
> We have two types of scanners.
>
> One is a Philips high capacity that we use to scan all slides and can 
> accommodate many 20-slide racks at once. It also requires a huge amount of 
> server storage space. We have 4 of those with the plan to scan up to 1000 
> slides per day. It would do your 50 slides in about an 90 minutes total in 
> one batch.
>
> We also have small scanner, a Mikroscan L5 that takes up to 6 slides in a 
> tray that we use to scan frozen sections for remote diagnosis. It is good for 
> random use all day in the gross room We have one at each of our three gross 
> rooms.
>
> Tim Morken
> Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
> Pathology UC San Francisco Medical Center
>
> -Original Message-
> From: Glover, Kimberly 
> Sent: Thursday, January 09, 2020 10:55 AM
> To: Morken, Timothy 
> Subject: RE: Need a Slide Scanner
>
> Tim,
>
> That is still to be determined. It will start as a small volume, maybe 20-50 
> slides a day just to collaborate.
>
> Kimberly
>
> -Original Message-
> From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu]
> Sent: Thursday, January 09, 2020 1:51 PM
> To: Glover, Kimberly
> Cc: histonet-requ...@lists.utsouthwestern.edu
> Subject: RE: Need a Slide Scanner
>
> Kimberly, how many slides will you be scanning daily?
>
> Tim Morken
> Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
> Pathology UC San Francisco Medical Center
>
> -Original Message-
> From: Glover, Kimberly via Histonet 
> Sent: Thursday, January 09, 2020 10:39 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Need a Slide Scanner
>
> Hi,
>
> I am interested in purchasing a slide scanner for my lab so we will have the 
> ability to share slides between sites. Can someone suggest a good scanner, 
> make/model?
>
> Kimberly Glover
> Product Manager, Histology and Hematology Warrington, PA
>
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> ---
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> Any issues can be reported to the Polysciences Helpdesk via emailing 

Re: [Histonet] plural fluid prep

[John Garratt via Histonet]

The cells have been living quite nicely in a natural culture medium at 37c so 
putting the pleural fluid at 4c will preserve the cells for a number of days. I 
do not think I would want to go past 3 to 4 days, but that is just the limit of 
my experience revisiting old ascitic fluid specimens.
You could use alcohol to preserve the specimen longer but not knowing what your 
experiment is I am loath to offer an opinion on that.

John

Sent from ProtonMail Mobile

On Wed, Dec 18, 2019 at 9:51 AM, Michelle Jamison via Histonet 
 wrote:

> Hi All,
> I work in a research lab where we are using plural fluid to proof a
> method. Recently we have received some, and are unsure how long it will
> be ok in the fridge, or if we should add some preservation fluid to it.
> Can anyone advise please.
> Thank you in advance, and Happy Holidays to all!!
> --
>
> Best of all things to you,
>
> Michelle Jamison
>
> //
>
> Tel : +1.435.752.6011 Ext. 435.227.1474
>
> m.jami...@elitechgroup.com  • www.elitechgroup.com
> 
>
> 
>
> Logo
>
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Re: [Histonet] guidelines for using a microwave designated for food, but used to heat up histogel

[John Garratt via Histonet]

With regard to microwaves themselves you may find requirements  related to 
monitoring for microwave radiation leakage in your local regulations and the 
recording of such inspections. It is a radiation device.

Trouble with your cytology IHC? Check how your cell blocks are being prepared 
and get rid of that microwave.

John

Sent from ProtonMail Mobile

On Thu, Dec 12, 2019 at 5:52 AM, Charles Riley via Histonet 
 wrote:

> There is no direct CAP regulation regarding the use of microwaves
> concerning food and chemical use. However, ANP.29430 says that microwaves
> used in the histology lab need to be under a fume hood to capture any
> vented chemical fumes. Also, I am not sure what the actual record is but
> I am sure there is an OSHA regulation that is part of the Chemical exposure
> regulations that states microwaves used in laboratories can not be used for
> both food and chemical processing.
>
> On Thu, Dec 12, 2019 at 8:37 AM Eileen Akemi Allison via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
>
>> Went on line to get MSDS regarding HistoGel. Here’s the link.
>> https://www.medline.com/media/catalog/Docs/MSDS/MSD_SDSD91968.pdf <
>> https://www.medline.com/media/catalog/Docs/MSDS/MSD_SDSD91968.pdf>
>>
>> Happy eating from a microwave which has been used at MD Anderson for
>> HistGel!
>>
>> Best regards,
>> Akemi
>>
>> > On Dec 12, 2019, at 5:18 AM, Kathleen S Cormier  wrote:
>> >
>> > Can I share that we had a pathologist (back in the day) use our lab
>> microwave to re-heat his coffee? It had Schiff’s reagent stains all over
>> the inside of it. Some people really don’t have any common sense…
>> >
>> >
>> > Kathy Cormier
>> > Core Leader
>> > Hope Babette Tang Histology Facility
>> > Koch Institute for Integrative Cancer Research at MIT
>> > 500 Main Street, 76-182
>> > Cambridge, MA 02139
>> > 617-258-8183
>> > corm...@mit.edu 
>> > https://ki.mit.edu/sbc/histology 
>> >
>> >
>> >
>> >
>> >
>> >
>> >
>> >
>> >
>> >> On Dec 12, 2019, at 7:12 AM, Eileen Akemi Allison via Histonet <
>> histonet@lists.utsouthwestern.edu > histonet@lists.utsouthwestern.edu>> wrote:
>> >>
>> >> Good morning histopeeps:
>> >>
>> >> We recently brought on an Ophthalmology Pathologist from MD Anderson
>> and they use histogel for orienting their thin eye specimens and need a
>> microwave to heat it up. We do not have a microwave in the lab for their
>> use but I had ordered one for them. I just found out they used the
>> microwave in our lab lounge to heat up histonet which was opened and being
>> stored in our gross area refrigerator.
>> >>
>> >> Do any of you know the CAP regulation against use of laboratory
>> reagents being used in a microwave being used for food consumption? I
>> ended up removing the microwave they used and put it in the histology lab.
>> Our Director wants me to write a formal letter to submit to him.
>> >>
>> >> Thank you in advance for your impute!
>> >>
>> >> Akemi Allison, BS, HT/HTL (ASCP)
>> >>
>> >>
>> >> ___
>> >> Histonet mailing list
>> >> Histonet@lists.utsouthwestern.edu > Histonet@lists.utsouthwestern.edu>
>> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> >
>>
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>>
>
> --
>
> Charles Riley BS HT, HTL(ASCP)CM
>
> Histopathology Coordinator/ Mohs
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Re: [Histonet] guidelines for using a microwave designated for food, but used to heat up histogel

[John Garratt via Histonet]

NEVER use a microwave to heat up Histogel regardless of ithe microwaves 
pedigree, especially for cytology specimens. There is a tendency to overheat 
the gel and thereby compromise the specimen, especially for IHC. Use a warming 
block where the temperature can be accurately controlled.

John

Sent from ProtonMail Mobile

On Thu, Dec 12, 2019 at 5:32 AM, Eileen Akemi Allison via Histonet 
 wrote:

> Went on line to get MSDS regarding HistoGel. Here’s the link. 
> https://www.medline.com/media/catalog/Docs/MSDS/MSD_SDSD91968.pdf 
> 
>
> Happy eating from a microwave which has been used at MD Anderson for HistGel!
>
> Best regards,
> Akemi
>
>> On Dec 12, 2019, at 5:18 AM, Kathleen S Cormier  wrote:
>>
>> Can I share that we had a pathologist (back in the day) use our lab 
>> microwave to re-heat his coffee? It had Schiff’s reagent stains all over the 
>> inside of it. Some people really don’t have any common sense…
>>
>>
>> Kathy Cormier
>> Core Leader
>> Hope Babette Tang Histology Facility
>> Koch Institute for Integrative Cancer Research at MIT
>> 500 Main Street, 76-182
>> Cambridge, MA 02139
>> 617-258-8183
>> corm...@mit.edu 
>> https://ki.mit.edu/sbc/histology 
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>> On Dec 12, 2019, at 7:12 AM, Eileen Akemi Allison via Histonet 
>>> >> > wrote:
>>>
>>> Good morning histopeeps:
>>>
>>> We recently brought on an Ophthalmology Pathologist from MD Anderson and 
>>> they use histogel for orienting their thin eye specimens and need a 
>>> microwave to heat it up. We do not have a microwave in the lab for their 
>>> use but I had ordered one for them. I just found out they used the 
>>> microwave in our lab lounge to heat up histonet which was opened and being 
>>> stored in our gross area refrigerator.
>>>
>>> Do any of you know the CAP regulation against use of laboratory reagents 
>>> being used in a microwave being used for food consumption? I ended up 
>>> removing the microwave they used and put it in the histology lab. Our 
>>> Director wants me to write a formal letter to submit to him.
>>>
>>> Thank you in advance for your impute!
>>>
>>> Akemi Allison, BS, HT/HTL (ASCP)
>>>
>>>
>>> ___
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>>> Histonet@lists.utsouthwestern.edu 
>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
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Re: [Histonet] Procedure for Blue Cheese for Fungus Controls

[John Garratt via Histonet]

If you have access to spare fresh tissue (ie placenta) you can infuse the 
tissue with fungus (blue cheese if you wish) and cultivate overnight before 
FFPE, I have found these make excellent control blocks and the tissue makes for 
good structural support, plus the tissue architecture might make the blue 
cheese more palatable to pathologists. Your idea is certainly not crackers.

John


www.ciqc.ca

‐‐‐ Original Message ‐‐‐
On Friday, November 22, 2019 5:01 PM, Akemi via Histonet 
 wrote:

> Hi Histopeeps! Here’s my procedure for Fungus Test Controls since we are out 
> of controls. This was from Blue Cheese I processed yesterday. It turned out 
> fabulous!!! Photo credit to Christina George (i know Histonet doesn’t attach 
> photos so can send to you) and lab credit to my staff, Quinnlan Dewitt and 
> Carlos Duran. I used President brand Le Blue which I purchased from Sprouts 
> grocery. It cost $7.95 for 3.5 oz. it was the most aged. I then took a 
> portion of the “moldy blue” cheese and put it in lens paper, then cassetted 
> it and fixed it in 10% Formalin in the morning for 8 hours and ran it 
> normally with routine surgicals. Thinking of publishing!
>
> Cheers!
>
> Sent from my iPhone
>
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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Re: [Histonet] Cell block processing

[John Garratt via Histonet]

There's been excellent discussion on fixation, IHC and cells blocks, with Joe 
Walker's email summing up the situation nicely. For those interested in side by 
side comparison of IHC staining of cytology cell blocks using different 
fixatives go to http://cpqa.ca/main/wp-content/uploads/2014/06/2014-Thomson.pdf

Regards

John Garratt


www.ciqc.ca

‐‐‐ Original Message ‐‐‐
On Monday, October 28, 2019 7:38 AM, Joe W. Walker, Jr. via Histonet 
 wrote:

> Hi Terri,
>
> At one time we did the same thing but have changed our approach in light of 
> the FDA's and CAP's view point on ASRs. The potential problem is that IHCs 
> are all validated/tested by the manufacturer on FFPE tissue. By introducing 
> methanol/ethanol as the first step in fixation, you potentially have altered 
> the initial fixation steps. I've attended several meetings on this topic and 
> have been advised to stop performing IHC on methanol/ethanol fixed specimens 
> unless we validated that this fixation step doesn't alter the expression of 
> the target antigen in the tissue. Formalin fixation after an alcohol fixation 
> doesn't change/reverse any alterations to the antigen in the tissue.
>
> We utilize an IBF tissue fixative but have also validated this fixative with 
> our antibody panels that we offer. The IBF does contain a small amount of 
> alcohol and the fixative is slightly different than 10% buffered formalin.
>
> I agree that CytoLyt is excellent at lysing red blood cells but would just 
> caution you on using the specimen for IHC without a disclaimer within your 
> report or validating your IHCs on these specimens to ensure they work as 
> expected. Keep in mind that most control tissue is FFPE and using it to 
> compare if the IHC worked in a first fixed alcohol specimen is not an apples 
> to apples comparison.
>
> Cheers,
>
> Joe W. Walker, Jr. MS, SCT(ASCP)
> Anatomical Pathology Manager
> joewal...@rrmc.org, www.rrmc.org
>
> -Original Message-
> From: Terri Braud via Histonet histonet@lists.utsouthwestern.edu
> Sent: Monday, October 28, 2019 10:14 AM
> To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Cell block processing
>
> [External Email] This email originated from outside of the organization. 
> Think before you click: Don’t click on links, open attachments or respond to 
> requests for sensitive information if the email looks suspicious or you don’t 
> recognize the sender.
>
> We collect our FNAs in CytoLyt. We also use it to wash all our non-gyn 
> fluids, but then we fix the cell block "pellet" in formalin.
> We have had no problems with immunos, and are able to lyse the RBCs to 
> provide a nice, clear specimen.
> Hope this helps.
>
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
>
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.com%2Fv3%2F__http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet__%3B!5JlSGkcjda6Eqs5J!rHP2_dFONxwRmR87ddavejT_o6RIcXBah8jw7nMUO0tWdC5KpksUnk2bttyIMkU%24=02|01|jwwalker%40rrmc.org|eeac9b3afe444de27cd208d75bb1634b|0e55647d438e4a448437e959c3cf2240|0|0|637078689748526729=1%2BpvxH9TcvKxAo6jgjr7fobCAUUz35sHbsLfoBvHmGE%3D=0
> [https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg]
>
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[Histonet] Fit-For-Purpose PD-L1 Biomarker Testing: Guidelines For Clinical Laboratories

[John Garratt via Histonet]

Fit-For-Purpose PD-L1 Biomarker Testing For Patient Selection in Immuno-Oncology

Guidelines For Clinical Laboratories From the Canadian Association of 
Pathologists-Association Canadienne Des Pathologistes (CAP-ACP)

Click 
[HERE](https://journals.lww.com/appliedimmunohist/Abstract/publishahead/Fit_For_Purpose_PD_L1_Biomarker_Testing_For.98668.aspx#pdf-link)
 to see the abstract publish ahead of print in Applied Immunohistochemistry & 
Molecular Morphology: [October 03, 2019 
-](https://journals.lww.com/appliedimmunohist/toc/9000/0)

   LinkedIn access  
https://www.linkedin.com/pulse/fit-for-purpose-pd-l1-testing-guidelines-clinical-john-garratt
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Re: [Histonet] Steel blade sharpening

[John Garratt via Histonet]

Way back 30 years ago when we moved to disposable blades I negotiated with the 
blade supplier to provide a blade holders for our microtomes for free. These 
blade holders replace the steel knife so you do not need to buy a new part for 
your microtome. If I remember right these blade holders were sold by Feather.

Good luck

John


www.ciqc.ca

‐‐‐ Original Message ‐‐‐
On Thursday, September 19, 2019 3:42 AM, Hannen, Valerie via Histonet 
 wrote:

> Good Morning all,
>
> We am coming out of the dark ages!! We are going to be transitioning to 
> disposable blades in the very near future. My main problem now , is that I 
> may not get the equipment( disposable blade holder or new Microtomes) that I
>
> will need soon enough ( we are running out of compounds needed to sharpen 
> these blades ourselves).
>
> I am waiting for review and approval of this equipment, so in the mean time I 
> may need to send my steel microtome blades out to be sharpened. We are on the 
> East Coast of Florida ( @ 1 hour away from Orlando).
>
> I am reaching out to you all asking if you know of any companies relatively 
> close to me that sharpen these blades.
>
> Thanks so much in advance for any help and insight you may give me.
>
> Valerie Hannen,MLT(ASCP),HTL,SU (FL)
> Section Chief, Histology
> Parrish Medical Center
> 951 N. Washington Ave.
> Titusville,Florida 32796
> T: (321)268-6333 ext. 7506
> F: (321) 268-6149
> valerie.hannen@parrishmed.commailto:valerie.han...@parrishmed.com
> www.parrishmed.com
>
> Histonet mailing list
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Re: [Histonet] pan-TRK or NTRK antibody

[John Garratt via Histonet]

VENTANA pan-TRK (EPR17341) Assay
Catalog Number: 790-7026

It should not be too difficult to find positive tumour controls since there is 
a high prevalence of NTRK in some cancers. ie Papillary thyroid cancer and 
secretary cancers ie secretary breast cancer (75%)
Normal testis should stain positive.
Endothelial cells should stain positive and will make a great internal control.

John Garratt

www.ciqc.ca



‐‐‐ Original Message ‐‐‐
On Wednesday, September 11, 2019 12:21 PM, Mark Tarango via Histonet 
 wrote:

> While we're on the topic of NTRK, does anyone have a positive case that
> they could share? I'm working up the FISH and have probes for NTRK1, 2 &
> 3. Any pan-NTRK positive IHC case would be great for detecting by FISH too.
>
> thanks!
>
> Mark Tarango
>
> On Wed, Sep 11, 2019 at 11:23 AM Piche, Jessica via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
>
> > Good Afternoon,
> > Where are people buying the pan-TRK or NTRK antibody from? Pre-dilute
> > preferred. Thank you in advance and have a great day!
> > Jessica Piche, HT(ASCP)
> > Waterbury Hosptial
> > Waterbury, CT 06708
> >
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> Histonet mailing list
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[Histonet] Immunohistochemistry Workshop in Vancouver

[John Garratt via Histonet]

For technologists, pathologists, residents, scientists or anyone else 
interested in immunohistochemistry.

On Friday, October 25, 2019, the cIQc will be holding our 2019 Technical 
Workshop: The Finer Details of IHC Readout.  Click here to 
[REGISTER](https://pathology-payment.med.ubc.ca/2019-ciqc-technical-workshop/)

- Based on the webinar series “Epitopically Speaking…” learn the important 
difference between reading and interpreting IHC staining in tissues.
- Learn how to design, implement and interpret the results of iCAPCs 
(immunohistochemistry Critical Assay Performance Controls ) to improve 
consistency and quality of staining in your laboratory.
- See a demonstration of the new online IHC readout proficiency modules to be 
offered by the cIQc/CAP-ACP that will provide on-going CME to technologists and 
pathologists. Participants at the workshop will receive free registration for 
one module of choice.
- Learn how to design and maintain a tissue control bank for IHC using four 
normal tissues that will cover the majority of your Class I testing.

Vancouver Eye Care Centre, 2550 Willow Street, Vancouver,  BC, Canada

www.ciqc.ca
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Re: [Histonet] Tissue scrolling

[John Garratt via Histonet]

The best I can offer is to advise you to wipe the knife holder area down with a 
cleaning solvent and use a fresh blade. Clean forceps with a solvent and place 
the scroll (50 microns) directly into a labelled 1.5mL plastic vial. I would be 
interested on other thoughts on this.

John



www.ciqc.ca




‐‐‐ Original Message ‐‐‐
On Friday, September 6, 2019 3:05 PM, Erin McCarthy via Histonet 
 wrote:

> Hello Histonetters,
>
> I am curious if any of you perform scrolling of paraffin embedded tissue
> for molecular studies? If so, what precautions do you take to minimize
> contamination? I am asking specifically about at the cutting station as
> that is my area of expertise. But we are looking to pilot this for samples
> that in the past have had large quantities of recuts.
>
> Thank you for any information you can provide!
>
> --
>
> Erin McCarthy, HT (ASCP)
> Histology Supervisor
>
> Tempus Labs
> 600 W. Chicago Ave.
> Chicago IL 60654
> Cell: (708)269-8610
>
> --
>
> This email and any attachments may contain privileged and confidential
> information and/or protected health information (PHI) that is protected by
> federal and state privacy laws.  It is intended solely for the use of
> Tempus Labs and the recipient(s) named above.  Nothing contained in this
> communication and any attachments thereto is intended to waive any
> privileges or rights of confidentiality.  If you are not the recipient, or
> the employee or agent responsible for delivering this message to the
> intended recipient, you are hereby notified that any review, dissemination,
> distribution, printing or copying of this email message and/or any
> attachments is strictly prohibited. * If you have received this
> transmission in error, please notify us immediately at (855)-442-8305 
> and permanently delete this email and any attachments*.
>
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Re: [Histonet] Tissue Contamination

[John Garratt via Histonet]

With regard to forceps: Do NOT use rat tooth or serrated forceps because even 
with rinsing there is potential for micro fragments to be trapped and carried 
over to the next sample. This also applies to forceps used at the tissue 
embedding stage. It is all about mitigating of risk.

John

www.ciqc.ca

‐‐‐ Original Message ‐‐‐
On Tuesday, August 27, 2019 8:36 AM, Joe W. Walker, Jr. via Histonet 
 wrote:

> We utilize small, disposable absorbent pads, which also absorb the formalin 
> fumes. We obtain ours through Leica/former Surgipath. They work well and are 
> changed in between cases. Each case utilizes a new scalpel blade and forceps 
> are rinsed in water between cases. I am not aware of any cross over of 
> tissues between cases when utilizing these practices.
>
> Joe W. Walker, Jr. MS, SCT(ASCP)
> Anatomical Pathology Manager
> joewal...@rrmc.org, www.rrmc.org
>
> -Original Message-
> From: Cartun, Richard via Histonet histonet@lists.utsouthwestern.edu
> Sent: Monday, August 26, 2019 2:48 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Tissue Contamination
>
> [External Email] This email originated from outside of the organization. 
> Think before you click: Don’t click on links, open attachments or respond to 
> requests for sensitive information if the email looks suspicious or you don’t 
> recognize the sender.
>
> What are people doing to ensure that there is no tissue carry-over on 
> instruments between cases when grossing? Thank you.
>
> Richard
>
> Richard W. Cartun, MS, PhD
> Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
> Proteomics Laboratory Director, Biospecimen Collection Programs Assistant 
> Director, Anatomic Pathology Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 972-1596 (Office)
> (860) 545-2204 (Fax)
> Richard.cartun@hhchealth.orgmailto:richard.car...@hhchealth.org
>
> This e-mail message, including any attachments, is for the sole use of the 
> intended recipient(s) and may contain confidential and privileged 
> information. Any unauthorized review, use, disclosure, or distribution is 
> prohibited. If you are not the intended recipient, or an employee or agent 
> responsible for delivering the message to the intended recipient, please 
> contact the sender by reply e-mail and destroy all copies of the original 
> message, including any attachments.
>
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> https://nam02.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet=02|01|jwwalker%40rrmc.org|101732ac49f34d82e9f408d72a560e09|0e55647d438e4a448437e959c3cf2240|0|0|637024421428296021=2dMuFge6C4Oaev7ZVeLvU1Rdn%2FgohOn2g1wKTJJi%2Fn4%3D=0
> [https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg]
>
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Re: [Histonet] Tissue Adherence problem

[John Garratt via Histonet]

Terri is right on the money with her advice.
Especially the de- ionized water,  NO double dipping and defiantly NO adhesive 
part.

John

Sent from ProtonMail Mobile

On Wed, Jul 17, 2019 at 10:25 AM, Terri Braud via Histonet 
 wrote:

> Hi Deanne -
> Hope this will help
> For our Ventana Ultra, we use Leica / Surgipath Apex Superior Adhesive slides.
> We use de ionized water in our baths at 45C ish, no additives.
> We never "double-dip" our slides (meaning that if we mount a control on the 
> top of the slide, we turn the slide upside down to pick it up, only immersing 
> the slide to pick up the intended tissue)
> We pre-bake IHC slides in a hot air blower over at 65C, 10 min for small 
> tissues, and 1 hr for large tissues, then load for IHC.
>
> For special stains, we use the same slides, but they go directly on the 
> Sakura Prisma stainer into the instrument oven at 65C for 15 minutes before 
> staining
> For H, we use the plain Surgipath Snowcoat slides, no adhesive, directly on 
> the Sakura Prisma stainer into the instrument oven at 65C for 15 minutes 
> before staining. They dry vertically in a rack as we cut, then we load as 
> soon as the rack is full.
>
> We once had an issue with tissue falling off and were able to trace it to a 
> new tech's liberal use of Paraguard Spray. Stopped using it, wiped everything 
> down with alcohol and our problem went away.
> Also, many years ago, I had a problem with a bad lot of slides, but these 
> were Cardinal's.
> I sincerely hope you can get this straightened out. I know how frustrating 
> such a problem can be.
> Terri
>
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
>
> Today's Topics:
> 2. Tissue Adherence Issue (Knutson, Deanne)
> Message: 2
> Date: Wed, 17 Jul 2019 09:21:49 -0500
> From: "Knutson, Deanne" 
> Subject: [Histonet] Tissue Adherence Issue
> Fellow Histonetters -
>
> I would appreciate your feedback on an intermittent issue that has shown up 
> in our lab.
> All of a sudden, we are having difficulty with tissue specimens falling off 
> of our slides on the IHC stains and special stains sporadically.
> About a year ago, we switched instruments on the IHC bench from the Leica 
> BOND to the ROCHE ULTRA, and we use the Ventana NexES special stainer.
> No adherence issues with all of the validation slides that were run.
> We have tried various types of slides recently as well, and the issue still 
> prevails.
>
> Would any of you mind telling me your slide workflow - type of slide used, 
> gelatin or not used in flotation bath, how long slides are cooked, etc 
> for your IHC slides, for your special stains slides, and even for your H 
> slides.
> I would welcome your suggestions and feedback.
>
> Thank you so much!!!
> Deanne Knutson
> Supervisor
> Anatomic Pathology
> "Let All Be Received as Christ."
>
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Re: [Histonet] FISH for breast core (Her2)

[John Garratt via Histonet]

Maxim, this is a link to the cIQc assessment page 
http://cpqa.ca/main/?page_id=24
If you look at the HER2 ISH assessments you will see numerous protocols used by 
laboratories. To my knowledge the protocols work on both biopsy and resection 
tissue.

John


www.ciqc.ca

‐‐‐ Original Message ‐‐‐
On Tuesday, June 11, 2019 11:18 AM, Пешков Максим via Histonet 
 wrote:

>
>
> Dear colleagues!
> Can anyone help? We are very appreciatated any suggestions, tips and hacks 
> for Her2 FISH onto breast trepine fixed in 10% NBF. It was here for more than 
> 7 days, then processed as usual into wax. IHC for Her2 has value as 2+.
> Sincerely,
> Maxim Peshkov,
> Russia,
> Taganrog.
>
> Histonet mailing list
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Re: [Histonet] Processing Schedule- ASP-6025

[John Garratt via Histonet]

The processing schedule looks fine. I am thinking that if you are having no 
problems with morphology on the H processing is not the issue. The extended 
period in the warming draw is a good hypothesis.
I do have a question. How long was that particular piece of tonsil kept in 
formalin before processing? Could over fixation be the issue?
I have found that maintaining tight control of control tissues is important 
which includes minimum and maximum fixation time.

John

Sent from ProtonMail Mobile

On Tue, May 7, 2019 at 5:52 PM, Greg Dobbin via Histonet 
 wrote:

> Fascinating Tony!
> We don’t typically leave them in the warming drawer any longer than a
> couple of hours, but maybe this particular tonsil was left linger for some
> reason and no one thought anything of it?! Something to consider for sure!
> Thanks.
> Greg
>
> On Tue, May 7, 2019 at 9:37 PM Tony Henwood (SCHN) <
> tony.henw...@health.nsw.gov.au> wrote:
>
>> Processing seems adequate.
>>
>> After processing, how long do they sit in the embedding centre block
>> holding tank before embedding?
>>
>> We found that quite a few antigens were affected when we stored control
>> tonsil in the embedding centre (dry) at 60oC for a few days before
>> embedding. In summary:
>>
>> Antibody Clone Dried (Normal = 3+)
>> CD4 4B12 0
>> BOB-1 TG14 0
>> CD3 LN10 1+
>> CD79a JCB117 1+
>> Oct-2 Oct-207 1+
>> CD8 4B11 2+
>> CD20 L26 3+
>>
>> So CD20 was unaffected but this process affected most of the antigens with
>> some losing antigen recognition by the antibody (eg CD4 and BOB-1).
>>
>> Another one of those pre-analytical issues we need to consider.
>>
>> And yes I am writing this up for submission!
>>
>>
>> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) |
>> Principal Scientist; Adjunct Fellow, School of Medicine, University of
>> Western Sydney; Visiting Lecturer, School of Life Sciences, Faculty of
>> Science, University of Technology Sydney | Histopathology
>> t: (02) 9845 3306 | f: (02) 9845 3318 | e: tony.henw...@health.nsw.gov.au
>> | w: www.schn.health.nsw.gov.au
>> m:
>>
>>
>> Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia
>> Locked Bag 4001, Westmead 2145, NSW Australia
>>
>> ♲ Please consider the environment before printing this email.
>>
>> -Original Message-
>> From: Greg Dobbin via Histonet [mailto:histonet@lists.utsouthwestern.edu]
>> Sent: Wednesday, 8 May 2019 5:07 AM
>> To: histonet@lists.utsouthwestern.edu
>> Subject: [Histonet] Processing Schedule- ASP-6025
>>
>> Hello colleagues,
>> I recently stained (IHC) a section of normal tonsil from another facility
>> with p16 and the resulting stain was better than the same stain on a
>> section of my labs own normal tonsil control.
>>
>> This has led us to question our processing schedule. I am not concerned
>> with our fixation because we fix everything for at least 24 hours in 10%
>> formalin (commercially prepared) prior to processing.
>>
>> Does anything jump out at you as being a potential red flag in the
>> following overnight protocol?
>>
>> - Formalin 15 mins; RT
>> - Processing water 1 min; RT
>> - ETOH 70% 30 mins; 35C
>> - ETOH 80% 30 mins; 35C
>> - ETOH 95% 30 mins; 35C
>> - ETOH 100% 30 mins; 35C
>> - ETOH 100% 40 mins; 35C
>> - ETOH 100% 60 mins; 35C
>> - Xylene 60 mins; 35C
>> - Xylene 60 mins; 35C
>> - Xylene 60 mins; 35C
>> - Paraffin 60 mins; 57C; vacuum
>> - Paraffin 60 mins; 57C; vacuum
>> - Paraffin 60 mins; 57C; vacuum
>>
>> Our formalin is changed after every 1100 cassettes and the alcohol,
>> xylenes and paraffins are managed similarly by the instrument. Our specimen
>> mix is a little of everything (skins, GIs, breasts, needle cores, gall
>> bladders, gyne, etc).
>>
>> The one unknown (so far) in this story, is how the tonsil from the other
>> laboratory was handled (ie the fixative used and for how long-I am assuming
>> 10% formalin).
>>
>> Obviously, many of you will have schedules that differ from this one, in
>> any number of ways, but what I am looking for from you is your opinion: *is
>> there anything about this schedule that is particularly concerning?* Thank
>> you, Greg
>>
>>
>> --
>> *Greg Dobbin*
>> 1205 Pleasant Grove Rd
>> 
>> RR#2 York,
>> PE C0A 1P0
>>
>>
>> *Everything in moderation...even moderation itself**!*
>> ___
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>>
>> This message is intended for the addressee named and may contain
>> confidential information. If you are not the intended recipient, please
>> delete it and notify the sender.
>>
>> Views expressed in this message are those of the individual sender, and
>> are not necessarily the views of NSW Health or any of its entities.
>>
> --
> *Greg Dobbin*
> 1205 Pleasant Grove Rd
> RR#2 York,
> PE C0A 1P0
>
> *Everything in moderation...even moderation itself**!*
> 

Re: [Histonet] Physcian office urine

[John Garratt via Histonet]

If you are referring to "screening urines" and not diagnostic (ie cystoscopy 
specimens)  my personal preference would be to get a series of three early 
morning specimens which have been kept refrigerated and delivered on ice. The 
urine was already slopping around the warm bladder for hours anyway. If there 
was anything atypical in the screening urine then get a better preserved 
specimen using your diagnostic urine protocol that would utilize a fixative 
like Cytolyt or whatever your preference.

John


www.ciqc.ca

‐‐‐ Original Message ‐‐‐
On Friday, April 26, 2019 3:16 AM, Hannen, Valerie via Histonet 
 wrote:

> Good morning, I am hoping to get your opinion on this. Do you send Saccommano 
> fluid to the doctor's offices for them to put into there urine for cytology 
> specimens or just have them refrigerate the specimens until delivered to the 
> Lab? My Lab Manager is asking which is best practice, we both have our 
> opinions, but they don't match.
>
> Happy Lab Week everyone!!
>
> Thanks so much,
>
> Valerie Hannen,MLT(ASCP),HTL,SU (FL)
> Section Chief, Histology
> Parrish Medical Center
> 951 N. Washington Ave.
> Titusville,Florida 32796
> T: (321)268-6333 ext. 7506
> F: (321) 268-6149
> valerie.hannen@parrishmed.commailto:valerie.han...@parrishmed.com
>
> www.parrishmed.com
>
> ===
>
> "This email is intended solely for the use of the individual to
> whom it is addressed and may contain information that is
> privileged, confidential or otherwise exempt from disclosure
> under applicable law. If the reader of this email is not the
> intended recipient or the employee or agent responsible for
> delivering the message to the intended recipient, you are
> hereby notified that any dissemination, distribution, or
> copying of this communication is strictly prohibited. If you
> have received this communication in error, please immediately
> delete this message. Thank you"
>
> =
>
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Re: [Histonet] her 2 controls

[John Garratt via Histonet]

I recommend that you e-mail these two gentlemen (both are super helpful) and 
they can put you in touch with your local distributor. Both their companies 
have HER2 controls plus controls for many other analytes.

mr...@statlab.com   (Mark Rees)

colin.trist...@histocyte.com


John


www.ciqc.ca

‐‐‐ Original Message ‐‐‐
On Thursday, April 18, 2019 9:15 AM, Nirmala Srishan via Histonet 
 wrote:

> Can someone suggest a company to purchase good Her 2 control slides.
> Preferably multi-tissue controls with +1,+2,+3.
> Thanks in advance.
>
> Nirmala Srishan
> Supervisor Histology
> Department of Pathology and Laboratory Medicine
> Holy Name Medical Center Teaneck, NJ
> Office: 201 541 6328
> Lab: 201 833 3023
> sris...@holyname.org
>
> Holy Name Medical Center is ranked among the top hospitals in the nation
> for patient care, clinical performance and workplace excellence.
> Click here to learn more.
>
>  Warning: The information contained in this message is privileged and
> CONFIDENTIAL and is intended only for the use of the addressee above. If
> you are not the intended recipient, you are hereby notified that any
> disclosure, copying, distribution, or taking of any action in reliance on
> the content of this message is strictly prohibited. If you have received
> this communication in error, please notify the sender by replying to this
> message, and then delete it from your system.
>
> Histonet mailing list
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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Re: [Histonet] [Histonet) Ki67 control

[John Garratt via Histonet]

My preference, whenever possible, is to steer people towards using normal 
tissues for controls in IHC. They are consistent in quality and appearance and 
fixation and processing can be better controlled.
I suggest you use Appendix for Ki67 and evaluate the lymphoid follicles plus 
there are some positive staining cells in the crypts.
Should you feel the need to use tumour and quantify the amount staining you 
could use Mantle Cell Lymphoma. This should give reasonably accurate 
consistency of Ki67 count through the block.
See the article "Standardization of Positive Controls.."   
https://www.researchgate.net/publication/282488454_Standardization_of_Positive_Controls_in_Diagnostic_Immunohistochemistry

I am most interested in "selling" the concept of iCAPCs ( immunohistochemistry 
Critical Assay Performance Controls) and any feedback is appreciated.

John

www.ciqc.ca

‐‐‐ Original Message ‐‐‐
On Monday, April 8, 2019 11:17 AM, Dessoye, Michael via Histonet 
 wrote:

> Hello Histonet!
>
> Looking for some advice regarding ER/PR/Her2 controls. I'm able to buy 
> commercially TMA blocks containing varying levels of positivity for ER, PR, 
> and Her2. This work nicely because you can have a negative and varying levels 
> of positive control as a same-slide control. But our docs would like to score 
> Ki-67 as well. Currently, they are just doing pos/neg for Ki67.
>
> It will take some time to collect cases and create a similar control, so I'm 
> wondering what y'all are using? Is there anything commercially available? Or 
> is everybody just collecting cases with varying degrees of positive?
>
> Thanks,
> Mike
>
> Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor 
> | Commonwealth Health Laboratory Services | 
> mjdessoye@commonwealthhealth.netmailto:mjdess...@commonwealthhealth.net | 575 
> N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 
> 570-552-1484
>
> Disclaimer: This electronic message may contain information that is 
> Proprietary, Confidential, or legally privileged or protected. It is intended 
> only for the use of the individual(s) and entity named in the message. If you 
> are not an intended recipient of this message, please notify the sender 
> immediately and delete the material from your computer. Do not deliver, 
> distribute or copy this message and do not disclose its contents or take any 
> action in reliance on the information it contains.
>
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Re: [Histonet] Fridge temp

[John Garratt via Histonet]

Given the cost and importance of IHC reagents the fridge should have a remote 
alarm on it. It should also be a lab grade fridge and NOT a domestic fridge 
which will have an auto defrost system to ruin your antibodies “automatically”.
I certainly would not trust a diagnosis or a drug therapy if there’s been 
improper antibody storage.
At a minimum use a data logger to monitor the fridge temperature . They cost 
less than $100.

John
Www.ciqc.ca

Sent from ProtonMail Mobile

On Tue, Mar 26, 2019 at 4:09 PM, MARY ANN via Histonet 
 wrote:

> Let's say, hypotheticaly, if you discover your fridge with all you antibodies 
> and detection kits were discovered to have been at 19c. for an undisclosed 
> amout of time. 12 24 48 hours due tona power surge..south Florida weather.
>
> Let's also propose your lab CFO/Owner dosent think its a big deal.
>
> First how would you handle the issue given the frisge has been restored ?
>
> Sent from Xfinity Connect Application
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Re: [Histonet] ER/PR/Her2 Controls

[John Garratt via Histonet]

I suggest you contact Mark Rees at StatLab.

mr...@statlab.com

John


www.ciqc.ca

‐‐‐ Original Message ‐‐‐
On Wednesday, March 20, 2019 12:42 PM, Dessoye, Michael via Histonet 
 wrote:

> Hello Histonet,
>
> Can anyone recommend a commercial source of ER/PR/Her2 control tissue (array 
> blocks would be preferred)? I'm looking for something similar to the breast 
> analyte control from Cell Marque, which is RUO:
>
> http://www.cellmarque.com/cms/histocyte/breast-DR.php
>
> Thanks,
> Mike
>
> Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor 
> | Commonwealth Health Laboratory Services | 
> mjdessoye@commonwealthhealth.netmailto:mjdess...@commonwealthhealth.net | 575 
> N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 
> 570-552-1484
>
> Disclaimer: This electronic message may contain information that is 
> Proprietary, Confidential, or legally privileged or protected. It is intended 
> only for the use of the individual(s) and entity named in the message. If you 
> are not an intended recipient of this message, please notify the sender 
> immediately and delete the material from your computer. Do not deliver, 
> distribute or copy this message and do not disclose its contents or take any 
> action in reliance on the information it contains.
>
> Histonet mailing list
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Re: [Histonet] Control tissue

[John Garratt via Histonet]

You will first need to document the control tissue you are collecting so you 
have the ischemic time, fixation time and processing schedule in your records. 
Make sure you control your fixation time and don't just grab tissue out of wet 
storage, this will save you a lot of grief. Then give each block a unique 
identifier that is referenced to the curated tissue. The more blocks you can 
collect from the original tissue the better. Ultimately your control slides 
will also carry this identifier so if you want to refer back to the collection 
process you can do.
Then take one of the blocks and stain it with a group of antibodies that will 
confirm the antigenicity of the tissue you collected. You then only need to 
test one block from the group of blocked you procured from the original tissue. 
I recommend you ultimately construct a compound block of tonsil, pancreas, 
Liver and appendix making sure each tissue is tested before creating the block. 
Of course the compound block is identified so you can reference back to 
pedigree of the individual tissue it was constructed with.

H to ensure there is normal tissue and no evidence of autolysis
IHC Testing on each block
Appendix – CD20, Desmin, Calretin
Tonsil – AE1/AE3, CD10, CD58
Pancreas – CK7, Insulin, CD56
Liver – HAS, CK19, Muramadase




For those with an interest in standardizing controls ( and who doesn't?) I 
recommend this article "Standardization of positive controls in diagnostic 
immunohistochemistry: recommendations from the international ad hoc expert 
committee." Applied Immunohistochemistry & Molecular Morphology 23, no. 1 
(2015): 1-18.  
https://www.researchgate.net/publication/282488454_Standardization_of_Positive_Controls_in_Diagnostic_Immunohistochemistry


Also there is series of 4 publications that are well worth reading. This is the 
link to Part 4, just to give you a taste.
Evolution of Quality Assurance for Clinical Immunohistochemistry in the Era of 
Precision 
Medicine:https://www.researchgate.net/publication/311565221_Evolution_of_Quality_Assurance_for_Clinical_Immunohistochemistry_in_the_Era_of_Precision_Medicine_Part_4




www.ciqc.ca

‐‐‐ Original Message ‐‐‐
On Monday, February 4, 2019 9:35 AM, Moe, Barbi A via Histonet 
 wrote:

> Could anyone share their process of finding and validating control tissues 
> for IHC?
>
> We currently use tonsil tissue as control tissue for 23 different antibodies 
> for IHC (CD2, CD3, CD4, etc.).
>
> My current process is to have our grossing personnel submit some extra tissue 
> from a surgical case. I cut an H section on the tissue to verify it has 
> fixed properly and is not needed for diagnosis. Once cleared by the 
> pathologist I cut the tissue into smaller sections to create individual 
> control blocks. I then cut a section of each block and test it for each 
> antibody. I can usually mount 6 sections on a slide - so in essence I am 
> checking 6 control blocks at one time.
>
> However, since the control block could be used for any of the 23 antibodies, 
> I check each antibody to make sure the tissue is good for all - so 23 slides 
> are being generated for every 6 blocks of control tissue made.
>
> Is this overkill? Do I need to check each block that is created? A 
> pathologist here feels that once he checks the H section and says the 
> tissue "should be good" that I shouldn't need to run each antibody and 
> generate all those slides.
>
> The CAP question ANP.21395 states that control tissue needs to be verified 
> and recorded as acceptable "prior to or concurrent with the reporting of 
> patient results."
>
> Does anyone verify and record their control tissue as acceptable "concurrent 
> with the reporting" - and not check it before using it?
>
> Any thoughts would be greatly appreciated as it is becoming more and more 
> difficult to obtain/verify/maintain control blocks for as fast as we are 
> using them due to increased workload!!
>
> Thanks in advance.
>
> Barb Moe
>
> Gundersen Health System
>
> La Crosse WI
>
> ba...@gundersenhealth.org if you'd like to respond individually
>
> Histonet mailing list
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Re: [Histonet] Opinion on dye on biopsies

[John Garratt via Histonet]

Be aware that validation of IHC should be performed if you are changing your 
processing protocol by adding a dye to the reagents or to a pre-processed 
tissue. Be cautious!


John



On Saturday, January 12, 2019 11:52 AM, Bob Richmond via Histonet 
 wrote:

> Gareth Davis asked about dyes to use to mark small GI biopsy specimens to
> make sure they're recovered during embedding.
>
> I've had good results marking small specimens with the solution of safranin
> O that's used in the microbiologists' Gram stain. Go to the micro lab and
> ask for a small amount of it and try it.
>
> Do not use eosin on biopsy specimens. Eosin's brilliant fluorescence makes
> it very difficult to do any kind of fluorescent stain on the sections. (It
> also doesn't work as well as safranin, which isn't fluorescent.)
>
> Another necessary procedure at the gross desk: fill out a log sheet that
> records the number of specimens you put into the cassette, and have that
> log sheet in front of you when you embed. (I've had a lot of histotechs
> flatly refuse to do this.)
>
> I like those little blue foam pads you put in the cassette and put the
> small specimens on. I usually cut them in two before putting them in the
> cassette.
>
> Bob Richmond
> Samurai Pathologist
> Maryville TN
>
> Histonet mailing list
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Re: [Histonet] FW: Pulmo Panel (+) Squamous Cell Lung ctrl

[John Garratt via Histonet]

The important thing is knowing which markers you are using, or wish to use. Are 
you looking at using diagnostics IHC markers or prognostic markers in your 
panel, or both?

John


www.ciqc.ca

‐‐‐ Original Message ‐‐‐
On Monday, January 7, 2019 9:54 AM, Linda Margraf via Histonet 
 wrote:

> Here is a message I am posting for Chrissy…..
> Begin forwarded message:
> From: "Altemus, Christine" < >
> Date: January 7, 2019 at 11:29:23 AM CST
> To: "lindamargraf@gmail.commailto:lindamarg...@gmail.com; 
> mailto:lindamarg...@gmail.com>
> Subject: Pulmo Panel (+) Squamous Cell Lung ctrl
>
> Hell o -
>
> Can you please post to Histonet website for me?
>
> I am looking for (+) Squamous cell lung cancer control for the Pulmo Panel 
> IHC Stains. Thank you!
>
> Chrissy
>
> St. Mary's Healthcare
>
> Amsterdam, NY
>
> Christine.Altemus@ascension.orgmailto:christine.alte...@ascension.org
>
> Histonet mailing list
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Re: [Histonet] IHC Negative reagent controls

[John Garratt via Histonet]

The article referenced below may be of interest.

Standardization of Negative Controls in Diagnostic Immunohistochemistry: 
Recommendations From the International Ad Hoc Expert Panel
https://www.researchgate.net/publication/261516067_Standardization_of_Negative_Controls_in_Diagnostic_Immunohistochemistry_Recommendations_From_the_International_Ad_Hoc_Expert_Panel


John

www.ciqc.ca

‐‐‐ Original Message ‐‐‐
On Wednesday, January 2, 2019 6:48 PM, Cayman Fleck via Histonet 
 wrote:

> A question that came up regarding negative reagent controls for 
> IHC...currently using Ventana i-View. Our regular negative control goes 
> through the standard antigen retrieval steps, like 99% of our antibodies. 
> However there are a small number of antibodies that require enzyme as well 
> (Protease 1). I've seen a number of suggestions regarding this for the 
> negative reagent control...some say use an additional negative control 
> protocol that includes the protease, some say to use a single negative 
> control protocol and just include the harshest cell conditioning that any of 
> your protocols use (so basically use the cell conditioning + protease 
> negative control for all antibodies)...i-View is not polymer-based so we need 
> to continue using negative controls. Any thoughts or advice?
>
> Frank
>
> Sent from Outlookhttp://aka.ms/weboutlook
>
> Histonet mailing list
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Re: [Histonet] Multi-Tumor Controls for IHC Validation

[John Garratt via Histonet]

For a basic multi-tissue control I recommend tonsil, liver, appendix and 
pancreas. Ensure you standardize fixation and processing time for the tissues 
you use

I suggest that labs use iCAPCs (immunohistochemistry critical assay performance 
controls) for developing your controls. This article (Standardization of 
Positive Controls in Immunohistochemistry) and will explain iCAPCs.
https://www.researchgate.net/publication/282488454_Standardization_of_Positive_Controls_in_Diagnostic_Immunohistochemistry

All the best for 2019

John

www.ciqc.ca

‐‐‐ Original Message ‐‐‐
On Monday, December 31, 2018 10:39 AM, Miranda Giorgi via Histonet 
 wrote:

> Hello,
>
> Does anyone use multi tumor tissue controls for their IHC validations? If so, 
> what do you use in them?
>
> We have been staining multi-tumor controls with our validation sets on top of 
> the 10 positive and 10 negative cases to test specificity, but we are 
> wondering if this is necessary. Plus, the blocks are sometimes difficult to 
> maintain. I'd love to hear what other labs are doing.
>
> Thank you,
>
> Miranda Giorgi, HTL (ASCP)cm
> Histology Manager
> Incyte Diagnostics
> 509-892-2744
>
> This e-mail and any attachments may contain CONFIDENTIAL information, 
> including PROTECTED HEALTH INFORMATION. If you are not the intended 
> recipient, any use or disclosure of this information is STRICTLY PROHIBITED; 
> you are requested to delete this e-mail and any attachments, notify the 
> sender immediately, and notify the InCyte Privacy Officer at 
> priv...@incdx.com or call (509) 892-2700.
>
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Re: [Histonet] IDH1 source

[John Garratt via Histonet]

If you check out the assessments page at www.ciqc.ca you will see EQA runs for 
IDH1. The protocols in the run reports will show the source of the Ab.

John

Sent from ProtonMail Mobile

On Sat, Dec 15, 2018 at 5:15 PM, Anne van Binsbergen via Histonet 
 wrote:

> Good morning to all
> Richard Cartun: we use IDH1 on FFPE tissue.
> We purchase from Dianova, Germany. (I believe they are still the only 
> suppliers worldwide).
> Purchased direct via their website.
> Vendors will also supply from the same source but at a higher price.
> Good luck
> Merry Christmas everyone!
> Anne
>
> Anne S van Binsbergen
> Supervisor, Histology Laboratory
> Pathology and Laboratory Medicine
> Sheikh Khalifa Medical City
> Abu Dhabi
> United Arab Emirates
>
> Sent from my iPhone
>
>> On Dec 15, 2018, at 10:00 PM, histonet-requ...@lists.utsouthwestern.edu 
>> wrote:
>>
>> Send Histonet mailing list submissions to
>> histonet@lists.utsouthwestern.edu
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>>
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>>
>> When replying, please edit your Subject line so it is more specific
>> than "Re: Contents of Histonet digest..."
>>
>>
>> Today's Topics:
>>
>> 1. IDH1 (Cartun, Richard)
>>
>>
>> --
>>
>> Message: 1
>> Date: Fri, 14 Dec 2018 20:38:38 +
>> From: "Cartun, Richard" 
>> To: "histonet@lists.utsouthwestern.edu"
>> 
>> Subject: [Histonet] IDH1
>> Message-ID:
>> <9215bd4b0ba1b44d962a71c758b68d2eac0e2...@hhcexchmb03.hhcsystem.org>
>> Content-Type: text/plain; charset="us-ascii"
>>
>> For those labs that are performing IDH1 IHC testing on FFPE tissue, which 
>> clone are you using and where do you get it? Thank you.
>>
>> Happy Holidays to everyone.
>>
>> Richard
>>
>> Richard W. Cartun, MS, PhD
>> Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
>> Proteomics Laboratory
>> Director, Biospecimen Collection Programs
>> Assistant Director, Anatomic Pathology
>> Hartford Hospital
>> 80 Seymour Street
>> Hartford, CT 06102
>> (860) 972-1596 (Office)
>> (860) 545-2204 (Fax)
>> richard.car...@hhchealth.org
>>
>>
>> This e-mail message, including any attachments, is for the sole use of the 
>> intended recipient(s) and may contain confidential and privileged 
>> information. Any unauthorized review, use, disclosure, or distribution is 
>> prohibited. If you are not the intended recipient, or an employee or agent 
>> responsible for delivering the message to the intended recipient, please 
>> contact the sender by reply e-mail and destroy all copies of the original 
>> message, including any attachments.
>>
>>
>> --
>>
>> Subject: Digest Footer
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>> End of Histonet Digest, Vol 181, Issue 5
>> 
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Re: [Histonet] Inconsistent H & E Staining

[John Garratt via Histonet]

A quick question: Are you using any recycled reagents? ie xylene, alcohol

John


www.ciqc.ca

‐‐‐ Original Message ‐‐‐
On Wednesday, November 14, 2018 12:24 PM, MONICA D. LOCKHART via Histonet 
 wrote:

> Hello Histo Fam!!
>
> Please help!
>
> My facility is experiencing frequent H & E staining problems. Everything from 
> cloudy cells on routine GI to "The Blank Look" on PNB's.
>
> We have made changes in some of the obvious areas like cassette whole size 
> and discontinued the use of sponges at grossing. However, with our biopsies, 
> we still see varying results in our H staining.
>
> Though this is not an exhaustive list of all the things we have encountered 
> or the number of changes we have made, hopefully this will give you an idea 
> of what we are seeing and maybe you have ran across this problem and can 
> share your wisdom.
>
> Any comments would be appreciated and most welcome.
>
> Monica D. Lockhart, BBA, HT (ASCP) PBT
> Supervisor Clinical Labs Histology
> Loyola University Medical Center
> 2160 S. First Ave, Bldg 110 Rm 2290
> Maywood, IL 60153
> (o) 708.327.2608
> (c) 708.692.8361
> monica.lockh...@luhs.org
>
> Confidentiality Notice:
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> recipient, please delete this message, and reply to the sender regarding the 
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>
> Histonet mailing list
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[Histonet] Canadian Labs - Let us know the challenges you face introducing PD-L1 testing

[John Garratt via Histonet]

We would like to request participation of ALL Canadian laboratories in this
survey. Even if you are not currently testing for PD-L1, please continue to
read. This survey is designed by the Canadian Immunohistochemistry Quality
Control scheme (cIQc) to understand: 

 i.  The challenges that laboratories face in introducing
PD-L1 testing, 

   ii.  The overall landscape in predictive biomarker
development in clinical IHC laboratories. 

 

Click on   
https://www.surveymonkey.com/r/P2XTCN8  to start the survey.

 

The information we receive from this survey will help cIQc develop  a
fit-for-purpose proficiency testing program and improve our ability to
assist laboratories.

 

The target participants for this survey are pathologists, laboratory
scientists/technologists,  researchers, and also oncologists  if they are
actively involved in predictive biomarker development. Therefore, please
feel free to forward  the survey link below to your oncologist colleagues,
especially if they work in close collaboration with you on new test
development. 

 

We would like to emphasize that this survey is open both to those who
currently test for PD-L1 and those who do not. Feedback from both groups is
important to us. 

 

Your participation in this survey is completely anonymous and none of the
responses will be connected to identifying information and the results will
be disseminated in aggregate form through cIQc. If the results are such that
we believe they may be of interest to the national/international community,
then we will also attempt to submit for publication in a peer-reviewed
journal.

 

The survey will take 8 - 10 minutes to complete.  

 

Click on   
https://www.surveymonkey.com/r/P2XTCN8  to start the survey.

 

The survey closes September 15th 2018

 

 

If you have any questions about this survey, or difficulty in accessing the
site or completing the survey, please contact  
john.garr...@ciqc.ca

 

 

Thank you in advance for your time and consideration.

 

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[Histonet] IHC Symposium in Quebec City

[John Garratt via Histonet]

2018 cIQc Symposium: IHC Toolkits for Diagnostic Pathology

Where:  Quebec City at the  Hotel Palace Royale (775 Boulevard
Honoré-Mercier)

When: September 20-21, 2018.

Go to   www.ciqc.ca  for more information.

 

The symposium is for Lab Scientists, Pathologists and Industry to discuss a
variety of topics including PD-L1, Alk, Ros1 

 

John Garratt – Canadian Immunohistochemistry Quality Control

 

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