Re: [Histonet] HTL Certification

[Rathborne, Toni via Histonet]

Hi Diana,

We have had two students complete the program at University of North Dakota. 
Both passed their certification exam the first time. You can get more 
information from this link.

 https://med.und.edu/histotechnology/admission-requirements.html

Best of luck finding a program that works for you!

Toni





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-Original Message-
From: Diana Martinez-Longoria via Histonet 
Sent: Thursday, June 27, 2024 12:48 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HTL Certification


*** This is an External Email ***

Good day all,


I have a question that hopefully I get some guidance on. I have an HT 
certification, but I wanted to try to study for the HTL certification, but it 
has been a very long time since I had to study for the boards therefore, I was 
wondering if you guys that are so knowledgeable can give me guidance on how to 
pursue my endeavor. I am a type of person that needs structure and an outline 
on how to study, so preferably I would need like an online school that can help 
me for the HTL.  Any recommendations?


Thank you,

Diana Martinez-Longoria

El Centro Regional Medical Center

Lead Histotechnician (ASCP)cm, B.S, A.S

Laboratory – Pathology Department
1415 Ross Ave | El Centro, CA  92243

(: 760.339.7267  | *: diana.martinez-longo...@ecrmc.org



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Re: [Histonet] IHC background

[Rathborne, Toni via Histonet]

We have not encountered a covertile artifact with our Bond III, which we've had 
for over 10 years. It may be that the covertiles need to be replaced or cleaned 
more, but we have not experienced this. We also place a control tissue at the 
top of the slide, and often have large patient sections on the lower portion. 
Rene had some good suggestions. I would also call Leica to see if they can 
offer suggestions over the phone. Their technical support team has been very 
helpful over the years.
Best of luck.




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-Original Message-
From: Regan Fulton via Histonet 
Sent: Thursday, September 29, 2022 3:42 PM
To: RENE MORALES 
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC background


*** This is an External Email ***

Hi,

The artifact you are seeing is a well-known artifact of the Leica covertile
design.
It does limit the available footprint for placing samples.

Hope this helps.

Regan



Regan Fulton, M.D., Ph.D.
CEO and Co-Founder
Array Science, LLC
475 Gate 5 Road, #100
Sausalito, CA 94965
(415) 577-7360



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On Thu, Sep 29, 2022 at 12:37 PM RENE MORALES via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Paula,
> Regardless of the position of the tissue: control/ patient ‘s tissue, most
> likely is the  distribution of the reagents during staining, isn’t
> adequate. Syringe, staining  reagents expiration, calibration, etc.,   Have
> you contacted service from Leica? Hope the solution is found,
> Thanks,
>
>
> Sent from my iPhone
>
> > On Sep 29, 2022, at 12:00, Paula via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
> >
> > Hello everyone,
> >
> >
> >
> > We have the Bond III and we ran an S100.  We placed 2 serial sections
> > vertically on the slide with the control tissue at the top for staining.
> The
> > patient's section at the top underneath the control tissue had background
> > staining, while the section on the bottom of that had no background
> > staining.  Can someone determine why this would happen?
> >
> >
> >
> > Thank you in advance.
> >
> > Paula
> >
> > Bio-Path Medical Group
> >
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Re: [Histonet] Stainer comparisons

[Rathborne, Toni via Histonet]

Rene,

I have the information from the vendors as you suggested. What I'm looking for 
is opinions. If you (or anyone else on histonet) have experience with either or 
both of these stainers, I'd like to know what you feel are the best features. 
Also looking to see what features are not as well praised or useful. Which 
specials do you run, and how do they compare to what you were previously using? 
How did getting one of these stainers impact your day-to-day operations? Did 
the positives outweigh the negatives, if there were any.

Thank you,
Toni

-Original Message-
From: RENE MORALES  
Sent: Monday, August 15, 2022 2:10 PM
To: Rathborne, Toni 
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Stainer comparisons


*** This is an External Email ***

Toni, the complete information can be found on the manufacturer specifications 
for these auto stainers. The only factor to be solved, no matter what 
instrument you will be selecting, is that you keep close monitoring of the 
service required for them. Important to be on the correct TAT.
Other factors are influencing the good results as well,
Thanks for sharing,
René Morales

Sent from my iPhone

> On Aug 15, 2022, at 07:23, Rathborne, Toni via Histonet 
>  wrote:
>
> 
> Good morning all,
>
> We're at the point where we are looking at replacing our H stainer, and I'm 
> wondering what opinions are out there about the Leica HistoCore Spectra ST - 
> High Throughput Routine & Special Stainer and the Sakura Prisma Plus Special 
> Stainer Configuration. What specials do you run on these stainers? Does this 
> significantly increase the TAT of the H slides? Overall, what do you like 
> or not like about either?
>
> Thank you,
>
> Toni
>
>
> 
>
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[Histonet] Stainer comparisons

[Rathborne, Toni via Histonet]

Good morning all,

We're at the point where we are looking at replacing our H stainer, and I'm 
wondering what opinions are out there about the Leica HistoCore Spectra ST - 
High Throughput Routine & Special Stainer and the Sakura Prisma Plus Special 
Stainer Configuration. What specials do you run on these stainers? Does this 
significantly increase the TAT of the H slides? Overall, what do you like or 
not like about either?

Thank you,

Toni




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Re: [Histonet] Microwave Reproducibility

[Rathborne, Toni via Histonet]

I would suggest starting with a standard amount of water (50-500 ml) to be 
tested at predetermined intervals according to your policy. Heat the water and 
record the temperatures at a minimum of 3 different times. Make sure that you 
are using a certified thermometer. From one test cycle to the next, you can 
note the deviation from your initial testing, and show reproducibility this way.




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-Original Message-
From: Willis, Donna G via Histonet 
Sent: Thursday, April 14, 2022 9:36 AM
To: Lora Jauregui ; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microwave Reproducibility


*** This is an External Email ***

What brand/type of microwave are you using?

Donna Willis
Anatomic Pathology Manager
Baylor Scott Health
Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas 75246
214-820-2465 office|214-725-6184 mobile



-Original Message-
From: Lora Jauregui via Histonet 
Sent: Thursday, April 14, 2022 8:26 AM
To: histonet@lists.utsouthwestern.edu
Subject: {EXTERNAL} [Histonet] Microwave Reproducibility


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Any recommendations on a protocol for testing for reproducibility on our 
microwave and documenting it for CAP?  We are a new lab and just setting up 
equipment and such. We only use it to heat water and to heat silver solution 
for our GMS stain.
Thank you

Sent from 
Mail for Windows

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Re: [Histonet] slide printers

[Rathborne, Toni via Histonet]

Jessica,

We did this successfully. At one time, our cassette printers (Leica IPC) were 
not interfaced either, but we were able to use Leica's software and enter the 
cassette identifiers, and that information was able to be saved in the 
bar-code, which also printed on the cassette. Patient names were not printed, 
only the accession number/letter.
We have the SlideMate Pro printers, and before they were interfaced, we were 
able to scan the barcode on our blocks and they would print the information 
obtained from there. The SlideMates were also able to be programmed so that the 
hospital name was printed at the bottom of each slide.
So it can be done, but only if your blocks are bar-coded.

Toni




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-Original Message-
From: P Sicurello via Histonet 
Sent: Friday, January 14, 2022 10:06 AM
To: Piche, Jessica 
Cc: Histonet 
Subject: Re: [Histonet] slide printers


*** This is an External Email ***

Hi Jessica,

Slide writers don't have to be linked to a LIS but without that you really 
don't save any time. All the information will be typed in manually.

On the plus side-you don't have to worry about trying to read someone's hand 
writing if you use a slide writer.

Sincerely,

Paula Sicurello

Sent from my iPhone

> On Jan 14, 2022, at 5:35 AM, Piche, Jessica via Histonet 
>  wrote:
>
> Good Morning,
>
> Happy Friday Everyone.
>
> I was wondering what people are using/recommending for slide printers at the 
> microtome? Do they all have to be connected to an LIS?
>
> Thank you,
>
> Jessica
>
> Jessica Piche, HT(ASCP)
> Histology Team Leader
> Waterbury Hospital Histology Laboratory
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Re: [Histonet] microtome blades and wax

[Rathborne, Toni via Histonet]

Jessica,

What has Leica said about the problem? I have always found them to be 
responsive and able to provide suggestions. This would be especially true with 
a new instrument, still under warranty. We have never had any inconsistencies 
with our Autocut, which was purchased last spring.

Toni




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-Original Message-
From: Piche, Jessica via Histonet 
Sent: Friday, January 14, 2022 8:43 AM
To: Histonet 
Subject: [Histonet] microtome blades and wax


*** This is an External Email ***

Good Morning,

We have been using Leica Histocore Autocut microtomes for a few months and have 
been having inconsistency with cutting on them. We have tried so many different 
blades, high and low, and have tried changing the blade angle. Nothing is a 
perfect fix, and we feel like we are really having to work much harder to get a 
great section. We were wondering what everyone else uses. I was also wondering 
if the wax could be an issue. We use EM-400 for embedding. I'd appreciate 
anyone's thoughts.

Thank you and have a nice weekend!

Jessica

Jessica Piche, HT(ASCP)
Waterbury Hospital Histology Laboratory
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[Histonet] Looking for vendor - 2-circle slides

[Rathborne, Toni via Histonet]

Our current vendor for off-set double-cytofunnel slides has discontinued their 
product. If anyone uses these, I'd appreciate your vendor's information & part 
number.

Vendors are welcome to reply directly to me!

Thanks all,

Toni



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and destroy any printouts.




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Re: [Histonet] FW: Tissue disposal

[Rathborne, Toni via Histonet]


Stericycle will also take your blocks and slides when you're ready to discard 
them.

Toni 





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-Original Message-
From: Carol G Fields via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, January 29, 2020 2:05 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] FW: Tissue disposal


*** This is an External Email ***

FYI…_
From: Carol G Fields
Sent: Wednesday, January 29, 2020 8:32 AM
To: 'Eileen Akemi Allison' 
Subject: RE: [Histonet] Tissue disposal


Hi,
Stericycle takes care of all your waste, including decanting and disposal.  
They are all over the US..Health & Safety alone plus employee time will justify 
you using these people.  No one should still be doing this…
I’ve used this company at several hospitals. They are great.
Good luck,
Carole
MLKCH
Los Angeles, CA

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Stericycle is a compliance company that specializes in collecting and disposing 
regulated substances, such as medical waste and sharps, pharmaceuticals, 
hazardous waste, and providing services for recalled and expired goods. It also 
provides related education and training services, and patient communication 
services. Wikipedia
Stock price: SRCL (NASDAQ) $63.65 +0.11 (+0.17%)
Jan 29, 10:57 AM EST - Disclaimer
Headquarters: Lake Forest, IL
CEO: Cindy J. Miller (May 2, 2019–)
Number of employees: 23,200 (2017)
Founded: 1989
Subsidiaries: Shred-it, Stericycle Environmental Solutions, MORE



-Original Message-
From: Eileen Akemi Allison via Histonet 
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, January 29, 2020 4:25 AM
To: Histonet 
mailto:Histonet@lists.utsouthwestern.edu>>
Cc: mirnaiba...@umcelpaso.org
Subject: [Histonet] Tissue disposal

Good morning Histopeeps! After reviewing our Policy Procedure Manual, our 
manager is questioning the bags we are using for disposing our tissues after 
diagnosis is complete.  We are separating the tissue from the formalin by 
straining the excess formalin and neutralizing it.  We then put the tissue into 
“Large Biohazard Bags” and the EVS staff picks it up and disposes it.  My 
manager is wondering if the “Biohazard Bags" are appropriate.  Should the bags 
state “Tissue” on the them?  Are there any special disposal bags available?

While working for a GI Lab in Monterey, CA we put all our bottle and tissue in 
"Biohazard Bags" and a Hazardous Waste company picked up the waste products 
from our endoscopy department along with ours.  Any comments and suggestions 
would be greatly appreciated.

Thank you in advance,
Akemi Allison, BS, HT/HTL


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Re: [Histonet] Fwd: [HISTONET] Biocare

[Rathborne, Toni via Histonet]

I got this reply from my Biocare rep.

We are not going out of business   What happened is over a year ago we were 
acquired by a Venture group, which is a good thing. They infuse money and are 
making us stronger.





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and destroy any printouts.

-Original Message-
From: Colleen Forster via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, September 12, 2019 1:37 PM
To: Maria Cruz
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Fwd: [HISTONET] Biocare


*** This is an External Email ***

I'd love to know who actually told Michael this? I've had a company try
this on me as wellinterested to know if it is the same company.

C. Forster

On Thu, Sep 12, 2019 at 12:27 PM Maria Cruz via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Mr. Bradley:
> This is how awful rumors get started.  I’m sure that Biocare is not going
> out of business.  They’ve recently released a new stainer and last weak an
> IVD-labeled antibody for P16.  If a sales rep from another company told you
> this they are pretty unethical and could learn a few things from the people
> at Biocare.
> Maria
>
> -- Forwarded message --
> From: Michael Bradley 
> To: Histopeeps Histonet 
> Cc:
> Bcc:
> Date: Tue, 10 Sep 2019 00:28:08 +0200
> Subject: [Histonet] Biocare
>
> Hi all
>
> I heard a rumor that Biocare is going out of business. Can anyone confirm
> or deny this?
>
> Thanks.
> Sent using the mail.com
>  mail app
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--
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
B173 PWB  612-626-1930
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Re: [Histonet] Cerner AB

[Rathborne, Toni via Histonet]

I too would be interested in this information.

Toni Rathborne







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and destroy any printouts.

-Original Message-
From: deGuzman, Jose R via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, April 24, 2018 5:10 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cerner AB


*** This is an External Email ***

Hello Histonetters.

I'm looking for opinions from users of Cerner's Advanced Barcode labeling and 
Tracking Software. I'd like to know your experience working with the software, 
and if you've worked with another software.

Thank you.

Jose de Guzman

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Re: [Histonet] cutting test

[Rathborne, Toni via Histonet]

If a "practical" interview is permitted, I would also have the applicant embed 
some control tissue. You can tell a lot about a person's habits by observing 
them actually work. Can they talk and embed/cut at the same time, or do they 
stop working? Are they conscientious about specimen identification, 
cross-contamination, and quality? Or do they just speed through their work 
without paying attention, or are they too slow for your expectations?
Quite often these traits will carry over to other areas of a person's work 
habits, and you may find that this method is beneficial.

Toni




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attachments, if any, is strictly prohibited. If you have received this 
transmission in error, please immediately notify the sender by telephone or by 
reply e-mail, and permanently delete this e-mail and the attachments, if any, 
and destroy any printouts.

-Original Message-
From: Terri Braud via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, January 09, 2018 9:23 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] cutting test


*** This is an External Email ***

Please be sure to clear any use of a "cutting test" during the interview 
process with your institution's legal department.  If an interviewee should cut 
themselves, the liability could be steep.  If you have a clearly defined 
minimum cutting speed/quality, a better solution would be to hire the best fit 
for your lab, then, if they can't meet  the minimum requirements, then it's 
easy to let them go during the probationary period.  That's what it's for.
Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

Today's Topics:
   2. Suggested Requirements for new uncertified HTs (Erin McCarthy)
   3. Re: Suggested Requirements for new uncertified HTs (Jay Lundgren)
   4. Re: Suggested Requirements for new uncertified HTs
  (Sanders, Jeanine (CDC/OID/NCEZID))

Message: 2
Date: Mon, 8 Jan 2018 10:19:00 -0600
From: Erin McCarthy  Hi All,

I work at a Start-Up and I was our first hire. I have been practicing since
2011 when I got my certification. However, we are looking to expand, what would 
you suggest our baseline technical competencies be for a position that focuses 
exclusively on cutting and IHC. In an interview it is hard to access what a 
person is capable of, would it be acceptable for me to have applicants performs 
a cutting time test?
I am open to hiring new techs, but I want to make sure we are not hamstrung by 
a great interviewer with a lacking technical skill set.
Thanks!
Erin McCarthy, HT (ASCP)
Histotechnician
Tempus Labs
600 W. Chicago Ave.
Chicago IL 60654
Ph:(312) 638-6344 Ext.3835



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Re: [Histonet] Histonet Digest, Vol 158, Issue 22 Breast Specimen

[Rathborne, Toni via Histonet]

There is also a CAP checklist standard that might help you to document these 
cases, without it seeming like you're complaining. Once the offenders have it 
brought to their attention, and know that it is being documented, they may 
start to give more consideration to the sections they're putting through.

**NEW** 07/28/2015
ANP.10038 Tissue Sample Quality Phase II There is a procedure that describes 
the process by which histotechnologists provide feedback to submitting 
pathologists and pathology assistants on the quality of the gross tissue 
sections received for tissue processing.
NOTE: Inadequate fixation, overly thick tissue sections, non-decalcified bone, 
the presence of staples, etc., can lead to poor quality histologic sections 
and/or poor quality special stains/special studies.
This requirement applies to both laboratories that gross tissue and perform all 
processing onsite, as well as laboratories that gross tissue and send it to 
another laboratory for processing, embedding, and sectioning (regardless of the 
outside laboratory's accrediting organization).
Records of such feedback and corrective action taken when problems are 
identified may be incorporated into the laboratory's quality management program.
Evidence of Compliance:
✓ Records of feedback and corrective action for problems identified with tissue 
quality


Toni


-Original Message-
From: Steve McClain via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, January 30, 2017 3:14 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Histonet Digest, Vol 158, Issue 22 Breast Specimen


*** This is an External Email ***

In my experience, rushing to process fatty or inadequately fixed specimens is a 
fool's game.
In my opinion, this problem cannot be solved by the histotechs- it begins with 
the grossers and is one for the pathologists to solve at the grossing bench.

Suggestion #1 Do nothing and let the medical director pathologist/ sign it 
out/deal w this individual case.

Suggestion #2  Sometimes a decent section can be obtained after changing 
paraffin.
[place the blocks in molds and melt the blocks and change to new paraffin- let 
them sit in the embedding center in the new paraffin for 60 minutes. Re-embed 
in new paraffin (2 changes) and then re-embed.]

Suggestion #3 Reprocess these blocks if permitted, recognizing that breast 
markers (if cancerous) may be erroneous.

Suggestion #4 Seek to prevent future occurrences by adjusting behavior at the 
grossing bench.
a) first ensuring adequate fixation and b) second ensuring adequate length/time 
of processing.


Steve A. McClain, MD
631 361 4000

What is the best way to handle Breast specimens that were grossed too thick and 
did not process well?  Our medical director does not want us to reprocess the 
tissue but it is almost impossible to get even a remotely decent section. If 
anyone has any other tips please let me know as soon as possible

--

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs**

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Re: [Histonet] Lab Coats and OCT

[Rathborne, Toni via Histonet]

Heather,

We are currently using a lab coat made by White Knight, which is fluid 
resistant. I don't know if it's any cooler, but they do protect what you're 
wearing under it.

Regarding the OCT. We also get our from Sakura, and have not noticed any change 
to the consistency. Is this happening with all lot numbers, or only one? I 
would check with Sakura. There may be a recall that you're not aware of. It 
might also have something to do with temperature/storage conditions.

Toni


-Original Message-
From: Seeley, Heather via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, February 18, 2016 11:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Lab Coats and OCT

Hello All,





Now that the hospital is enforcing us to wear lab coats all the time, wondering 
if anyone has suggestions for some that aren't too hot, the ones we have now 
are making us sweat!



Also, we have been having issues with our OCT, we currently use the Sakura OCT 
and have use it for years. Within the last 6 months or so it has become like 
glue on the chucks even if we let it soak in hot water and scrub with a brush, 
it just doesn't want to come out! If anyone has any suggestions on brands to  
try we would appreciate it!

Thanks!


HEATHER SEELEY, HT(ASCP)
Histotechnologist
803-985-4676 OFFICE
803-327-7598 FAX

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Re: [Histonet] Temperatures

[Rathborne, Toni via Histonet]

Use a NIST certified thermometer as you would with other lab thermometers for 
comparison. 

Toni


-Original Message-
From: Walter Benton via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, February 03, 2016 11:57 AM
To: Fawn Bomar; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Temperatures

Secondary thermometer in paraffin reservoir of  embedding center.

-Original Message-
From: Fawn Bomar via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, February 03, 2016 11:23 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Temperatures

Hi All,



I was wondering how everyone tracked their cryostat and paraffin temperatures.  
Do you all just use the machine temperature, or do you all use the machine and 
a second thermometer as verification?



Thank you

Fawn
-
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[Histonet] OR specimens in formalin

[Rathborne, Toni via Histonet]

Good afternoon.

This question is mainly for those who attended NSH this year, but others may 
know what I'm looking for too. In the Exhibition Hall, there was a product 
which was to be used in the OR and in which the specimen was placed in a bag, 
which itself was in an instrument and was then filled with formalin depending 
on the size of the specimen. Although I didn't think that we had a need for it 
at the time, there has been some interest expressed by the OR now. If anyone 
remembers the name of this instrument or the company that sells it, I'd be 
grateful if you could respond.

Toni



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Re: [Histonet] OR specimens in formalin

[Rathborne, Toni via Histonet]

Thanks everyone for your quick responses! The product I was looking for was 
SealSAFE by Milestone.

Toni


_
From: Rathborne, Toni
Sent: Wednesday, November 11, 2015 12:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: OR specimens in formalin


Good afternoon.

This question is mainly for those who attended NSH this year, but others may 
know what I'm looking for too. In the Exhibition Hall, there was a product 
which was to be used in the OR and in which the specimen was placed in a bag, 
which itself was in an instrument and was then filled with formalin depending 
on the size of the specimen. Although I didn't think that we had a need for it 
at the time, there has been some interest expressed by the OR now. If anyone 
remembers the name of this instrument or the company that sells it, I'd be 
grateful if you could respond.

Toni



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Re: [Histonet] embedding center backup

[Rathborne, Toni]

Stacy,

Our volume is similar, and we do have a backup embedding center. I'm sure that 
you could get a used one for much less than a new one to have just for this 
purpose.

Toni

-Original Message-
From: Stacy McLaughlin [mailto:stacy_mclaugh...@cooley-dickinson.org] 
Sent: Monday, May 18, 2015 2:59 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] embedding center backup

Happy Monday in Histoland,
How many of you out there have a backup embedding center or paraffin dispenser 
for a backup?  We are a smaller lab (10k surgical cases/year).  We only have 
one embedding center and I want to ask what others out there are doing.
Thank you,
Stacy

Stacy McLaughlin, HT(ASCP)
Histology Supervisor
Cooley Dickinson Hospital
30 Locust Street
Northampton,MA 01060
stacy_mclaugh...@cooley-dickinson.org

[Cooley-Dickinson.org]
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Re: [Histonet] Histonet Digest, Vol 138, Issue 16

[Rathborne, Toni]

Yes, the last one ([Histonet] cpt codes for quantitative and semi quantitative 
ihc )had no content.

-Original Message-
From: Myra Huth [mailto:mh...@baymedical.org] 
Sent: Wednesday, May 13, 2015 1:04 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Histonet Digest, Vol 138, Issue 16

Is anyone else only receiving blank pages from histonet?  Thanks

-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 
[mailto:histonet-requ...@lists.utsouthwestern.edu]
Sent: Wednesday, May 13, 2015 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 138, Issue 16


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Center Sacred Heart Health System, its subsidiaries and affiliates, are 
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may contain privileged, proprietary, or confidential information (i.e., 
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RE: [Histonet] reference lab for diagnosis of Hirschsprung's disease in an adult patient

[Rathborne, Toni]

http://www.mayomedicallaboratories.com/test-catalog/Clinical+and+Interpretive/80573

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Walter Benton
Sent: Friday, March 20, 2015 2:21 PM
To: Powers, Kerry; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] reference lab for diagnosis of Hirschsprung's disease 
in an adult patient

http://www.mayomedicallaboratories.com/test-catalog/search.php?search_type=diseasesearch=Hirschsprung+Disease

https://www.genetests.org/search/tests.php?search=Hirschsprung%20Disease,%20Sequencing%20RET%20Genesubmit=Searchstart=0types%5B%5D=from=tests

https://aravindachakravartilab.org/pro/Genetic_Testing_And_Counseling.html


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Powers, Kerry
Sent: Friday, March 20, 2015 1:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] refernce lab for diagnosis of Hirschsprung's disease in an 
adult patient

We are looking for a reference lab, in the united states, that can help us with 
the diagnosis of Hirschsprung's disease in an adult patient.  Any assistance 
would be greatly appreciated.

Kerry Powers, HTL(ASCP)
Histology Supervisor
Comanche County Memorial Hospital
3401 W. Gore Blvd
Lawton, Ok 73505
P: 580-355-8699 ext 3359
F: 580-585-5462
powe...@ccmhonline.com

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RE: [Histonet] Masson trichrome and H and E

[Rathborne, Toni]

We have been very satisfied with special stain solutions from Poly Scientific 
(http://www.polyrnd.com/products/stains/general/aniline-blue---masson's-trichrome.aspx).
 We get an 8oz bottle for under $30. Very good customer service too.

Toni

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Brown
Sent: Tuesday, March 03, 2015 7:39 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Masson trichrome and H and E

Hello!

I am doing Masson trichrome manually (not with a machine) and I just found out 
the kit is $800!! I was thinking of buying the ingredients separately, but why 
the hell is it so expensive???
Also, the people I was going to borrow their reagents from said their aniline 
blue is not very good and I wanted to replace it.  I only need about 250ml, 
what brand do you guys prefer?
l know I can google this, but I want to know what you guys like and what works 
best.  This is for mouse kidney paraffin sections, 4 to 5 microns.
Another question, I did H and E and there is no eosin staining.  I think the 
reagents are pretty old, so I thought that might be a problem.  Also because my 
lab is cheap, they were reusing the xylenes and EtOH for both rehydrating and 
rehydrating.  I told my boss this is probably not a good idea as the end steps 
will have stain in them.  And I also think this is why it didn't work! The EtOH 
is also really old so who know is the 100% is actually even close to 100% any 
more.  I'm buying new reagents, but if you guys think anything else would help, 
let me know.

Also, shoutout to Ann, I know you're reading these!! Join the list!!

Emily


By bitching and bitching and bitching, they could exhaust the drama of their 
own horror stories. Grow bored. Only then could they accept a new story for 
their lives. Move forward.

-Chuck Palahniuk, Haunted
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[Histonet] RE: Which kind of blade carrier?

[Rathborne, Toni]

Did you have this microtome in to demo? If so, what was used at that time? If 
not, is there an opportunity to do so before getting a PO? I can tell you that 
every microtome, tissue processor, stainer, coverslipper, and IHC instrument we 
have ever purchased has been brought in as a demo first.  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lake, Kim S
Sent: Friday, February 20, 2015 9:36 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Which kind of blade carrier?

I received approval to order a new microtome (yay!), and we have chosen a 
Thermo Scientific HM 300 Series Rotary Microtome to replace our Leitz 1512. My 
question is, which kind of blade carrier should I choose, the E or ER? We use 
high profile disposable blades. I can't tell from the tiny pictures what the 
difference is, and a search of histonet didn't help. Anyone have any advice?

Thanks!

Kim Lake, MLS (ASCP)CM
Laboratory Manager
Oral Pathology Laboratory
University of Iowa College of Dentistry


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RE: [Histonet] Bone Saw

[Rathborne, Toni]

We use a device from Mopec.

http://media3.mopec.com/media/pdf/AutopsyAccessories(Page76).pdf

It's manual, but works great.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jason McGough
Sent: Tuesday, February 17, 2015 3:59 PM
To: Mike Pence; histonet-boun...@lists.utsouthwestern.edu; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Bone Saw

We use a Dremel tool. It works great!!



Jason McGough, HT(ASCP)

Operations Manager

Clinical Laboratory of the Black Hills

605-343-2267

jmcgo...@clinlab.com mailto:jmcgo...@clinlab.com 

www.clinlab.com http://www.clinlab.com 

 
 
-Original message-
 From:Mike Pence mpe...@grhs.net mailto:mpe...@grhs.net 
 Sent: Tuesday, February 17, 2015 1:56 PM
 To: histonet-boun...@lists.utsouthwestern.edu 
 mailto:histonet-boun...@lists.utsouthwestern.edu ; 
 histonet@lists.utsouthwestern.edu mailto:histonet@lists.utsouthwestern.edu 
 Subject: [Histonet] Bone Saw
 
 I am trying to see what everyone is using at your grossing station for bone 
 saw to cut femoral heads and toes for osteo. If you are using a Stryker saw 
 how are you holding the specimens to make good thin sections?
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 http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
 
 


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[Histonet] RE: Peloris Processor

[Rathborne, Toni]

I would contact Leica and ask. They're very good about sending electronic 
versions of their manuals.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
Sent: Thursday, February 12, 2015 12:48 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Peloris Processor

Hello to all in histoland.  Does anyone have a SOP on how to operate the 
Peloris processor?  Any help will be greatly appreciated.


Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
713-566-5287(Lab)
713-566-2148(Office)

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[Histonet] RE: Sterile slides for FNA

[Rathborne, Toni]

What a great resource. How does Sterile Processing package them for you? 

-Original Message-
From: Mike Pence [mailto:mpe...@grhs.net] 
Sent: Wednesday, February 11, 2015 9:42 AM
To: Rathborne, Toni; histonet@lists.utsouthwestern.edu
Subject: RE: Sterile slides for FNA

We make our own sterile slides. We package 2 slides per package and send to 
sterile processing where then sterilize them for us. We then let the 
radiologist smear/touch/do their thing on the slide and the needle remains 
sterile. You can make up 200 packets in just a few minutes and save a lot of $$.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Wednesday, February 11, 2015 8:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Sterile slides for FNA

Good morning everyone.

I was wondering if I could get some feedback about whether anyone uses sterile 
slides for FNAs, if you do them. While most of the radiologists transfer the 
core from the end of the needle onto the slide by using a scalpel to assist, we 
have one radiologist who prefers to place the core directly onto the slide by 
using the needle. This is fine, unless he needs to go back in and make another 
pass. If the needle has touched a slide which is not sterile, then the needle 
would have to be changed to prevent contamination of the site. Does anyone use 
sterile slides, and are these available in a small quantity so that there is no 
waste?
Vendors are welcome to reply to me directly, unless others would like the 
information as well.

Toni


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[Histonet] Sterile slides for FNA

[Rathborne, Toni]

Good morning everyone.

I was wondering if I could get some feedback about whether anyone uses sterile 
slides for FNAs, if you do them. While most of the radiologists transfer the 
core from the end of the needle onto the slide by using a scalpel to assist, we 
have one radiologist who prefers to place the core directly onto the slide by 
using the needle. This is fine, unless he needs to go back in and make another 
pass. If the needle has touched a slide which is not sterile, then the needle 
would have to be changed to prevent contamination of the site. Does anyone use 
sterile slides, and are these available in a small quantity so that there is no 
waste?
Vendors are welcome to reply to me directly, unless others would like the 
information as well.

Toni
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RE: [Histonet] Frozen Sections

[Rathborne, Toni]

We will provide assistance weekdays with staff that accession and stain the 
case.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald
Sent: Monday, February 09, 2015 10:51 AM
To: Mike Pence; 'lsmal...@juno.com'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Frozen Sections

we do, during the day Monday - Friday (8:00-17:30) but not over the weekend

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence
Sent: Monday, February 09, 2015 9:05 AM
To: 'lsmal...@juno.com'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Frozen Sections

We provide assistance while the dept. is staff with histology techs.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
lsmal...@juno.com
Sent: Friday, February 06, 2015 9:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Frozen Sections

Hi, I need to ask Histoland a questionHow many HT departments provide 
assistance to the pathologist in the performance of frozen sections (cutting 
and staining of slides) to be evaluated by the pathologist?   Thank you very 
much in advance!  
Lorraine


Fast, Secure, NetZero 4G Mobile Broadband. Try it.
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[Histonet] RE: Storage of paraffin blocks

[Rathborne, Toni]

To comply with Joint Commission standards, ours are stored 6 off the floor.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar
Sent: Tuesday, February 03, 2015 8:48 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Storage of paraffin blocks

Hi everyone,



I hope all is going well in the new year.  I was wondering if everyone that 
stores their paraffin blocks in the plastic tissue tek block filing cabinets, 
stores these cabinets directly on the floor or do you all place them on some 
sort of stand?



Thank you

Fawn
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[Histonet] FW: VIP 5

[Rathborne, Toni]

Thank you everyone, I now have the manual. Amazing response time! 

Toni

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Tuesday, January 20, 2015 2:39 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] VIP 5

I'm looking for a copy of a User's Manual for our VIP 5. If anyone has one that 
they could send it would be greatly appreciated!

Toni

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[Histonet] VIP 5

[Rathborne, Toni]

I'm looking for a copy of a User's Manual for our VIP 5. If anyone has one that 
they could send it would be greatly appreciated!

Toni

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[Histonet] Research cases

[Rathborne, Toni]

Good morning everyone!

I wanted to ask what others are doing about charges when blocks  slides are 
being requested for research purposes. We have been asked to provide a cost for 
this service, because the research companies are willing to pay the lab for our 
time processing, embedding, and cutting slides specifically for this purpose. 
Any guidance in this would be greatly appreciated!


Toni Rathborne
Pathology Supervisor
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[Histonet] RE: Billing codes 2015

[Rathborne, Toni]

I found this in my inbox right after your email Marilynn. Thought it would be 
worth sharing with everyone.

Toni


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
rmweber...@comcast.net
Sent: Friday, December 05, 2014 8:43 AM
To: histonet
Subject: [Histonet] Billing codes 2015


Hi,  I was wondering if anyone has heard of any new billing codes for 2015 in 
regards to cytology, urology pathology or immunohistochemistry.  If so, could 
you please share? 
  
Thanks so much for your help. 
  
Marilynn Weber H.T.(ASCP)QIHC 
Coastal Pathology Consulting Services LLC 
732 814-1170 
fax 267 722-8308 

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[Histonet] RE: Water baths

[Rathborne, Toni]

We use whatever is the narrowest range for the reagents stored in there.  So if 
there is one reagent with a range of 4-10°C, and another with 2-8°C, we'll use 
4-8° as our range.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Timm, Amber L
Sent: Wednesday, December 03, 2014 11:09 AM
To: Hannen, Valerie; 'Weems, Joyce K.'; 'Abbott, Tanya'; 
histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Water baths

What is everyone using as their histo refrigerator temperature range?

Amber Timm, MLS (ASCP)CM

Supervisor of Laboratory Technical Services

The Diagnostic  Treatment Center

3401 Cranberry Blvd.

Weston, WI 54476

Phone: 715-393-2485

Email: ti...@dxandtx.commailto:ti...@dxandtx.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie
Sent: Tuesday, December 02, 2014 10:50 AM
To: 'Weems, Joyce K.'; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Water baths

We still do, due to the fact that it is on our general temperature chart that 
is used in the department.


Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.commailto:valerie.han...@parrishmed.com
www.parrishmed.comhttp://www.parrishmed.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Tuesday, December 02, 2014 11:20 AM
To: 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Water baths

It is no longer a CAP requirement to do this, so we haven't for many years.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.orgmailto:joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.orghttp://www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
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review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya
Sent: Tuesday, December 02, 2014 10:29 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Water baths

Does anyone take daily temperature checks of their water bath (used for cutting 
only) for CAP?

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 
19603-0316 ph  610-378-2635 fax 610-898-5871
email: tanyaabb...@catholichealth.netmailto:tanyaabb...@catholichealth.net

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[Histonet] RE: re: Flotation bath temperatures

[Rathborne, Toni]

Although it is not in the Anatomic CAP checklist, there is a new All Common 
question that does address this, and which, if I'm understanding it, would 
include flotation baths.

 
All Common checklist, COM.30750, Phase II

Temperatures are checked and recorded each day of use for all 
temperature-dependent equipment and environments using a calibrated 
thermometer.

Toni

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth 
Chlipala
Sent: Wednesday, December 03, 2014 2:19 PM
To: Teri Johnson; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: re: Flotation bath temperatures

Teri

We do, it's more of a combination use log for the microtome and tissue 
flotation bath, we record temperature and that we cleaned the unit, used fresh 
water and also cleaned the microtome.  This document also records the yearly 
calibration of the floatation bath and the yearly PM of the microtome.  I do 
need to add that both the calibration and PM will have additional documentation 
that is stored in a designated binder.  I can send you a copy of the form in a 
separate e-mail if you would like it.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

March 10, 2014 is Histotechnology Professionals Day

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teri Johnson
Sent: Wednesday, December 03, 2014 12:04 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] re: Flotation bath temperatures

Dear all,

I am changing the language to flotation bath (instead of water bath, since that 
is entirely different lab equipment). I understand there is no longer a CAP 
requirement to record temperatures. Is there a GLP (or GxP) requirement to do 
so?

Teri Johnson, HT(ASCP)QIHC
Manager Clinical Trial Testing
Genoptix, Inc.
SAN5, Rm. 2005
760.516.5954 (office)
760.516.6201 (fax)




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[Histonet] RE: Water baths

[Rathborne, Toni]

In the All Common checklist, COM.30750, a Phase II, addresses this and it is a 
requirement.

Temperatures are checked and recorded each day of use for all 
temperature-dependent equipment and environments using a calibrated 
thermometer.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie
Sent: Tuesday, December 02, 2014 11:50 AM
To: 'Weems, Joyce K.'; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Water baths

We still do, due to the fact that it is on our general temperature chart that 
is used in the department.


Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
www.parrishmed.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Tuesday, December 02, 2014 11:20 AM
To: 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Water baths

It is no longer a CAP requirement to do this, so we haven't for many years.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya
Sent: Tuesday, December 02, 2014 10:29 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Water baths

Does anyone take daily temperature checks of their water bath (used for cutting 
only) for CAP?

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 
19603-0316 ph  610-378-2635 fax 610-898-5871
email: tanyaabb...@catholichealth.net

This email and attachments contain information that may be confidential or 
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and delete this message completely from your information system. Further use, 
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privilege or other confidentiality protections.
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[Histonet] RE: Histonet Digest, Vol 132, Issue 12

[Rathborne, Toni]

As part of reporting all work related injuries, we investigate the cause or 
contributing factor and determine if the injury could have been avoided, and 
how to do so in the future. These types of injuries happen infrequently, so we 
have not done regular in-services. It is a good idea though, and should be 
something to consider.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barr,Kaye H
Sent: Tuesday, November 11, 2014 4:44 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Histonet Digest, Vol 132, Issue 12

Does anyone do a safety inservice for lab personnel regarding cuts/lacerations 
received when performing microtomy (paraffin  FS)?


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: Tuesday, November 11, 2014 12:52 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 132, Issue 12

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Contents of Histonet digest...


Today's Topics:

   1. CPT Code changes (Weems, Joyce K.)
   2. Re: Microm 335E Retraction (Mike Andrews)
   3. RE: Microm 335E Retraction  (Vernon, Richard I.)
   4. Validations- Retrospective,   Concurrent and Prospective and
  Revalidations (ian bernard)
   5. Texas Society for Histotechnology 2015Sumposium/Convention -
  Plano, Texas (kdwyer3...@aol.com)
   6. RE: Histonet Digest, Vol 132, Issue 10 (Richard Bowker)


--

Message: 1
Date: Mon, 10 Nov 2014 21:39:22 +
From: Weems, Joyce K. joyce.we...@emoryhealthcare.org
Subject: [Histonet] CPT Code changes
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID:

e3a4ebd57a691646bcced4aa5911a030011c3c7...@e14mbx12n.enterprise.emory.net

Content-Type: text/plain; charset=us-ascii

Have you all seen this news?


To eliminate these discrepancies, CPT(r) 2015 revises parent code 88342, 
imunohistochemistry or immunocytochemistry, per specimen; initial single 
antibody stain procedure, deletes indented code 88343, and adds two new 
indented codes, +88341, each additional single antibody stain procedure, and 
88344, each multiplex antibody stain procedure.


Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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--

Message: 2
Date: Mon, 10 Nov 2014 21:16:32 -0600
From: Mike Andrews udsd...@gmail.com
Subject: Re: [Histonet] Microm 335E Retraction
To: Stoll, Kathryn kst...@mcw.edu
Cc: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID: 228c4e7f-1815-42e0-bfe5-519a92bfe...@gmail.com
Content-Type: text/plain;   charset=us-ascii

A complete user manual is available at 
http://www.bu.edu/becf/downloads/BioInterface%20Technologies/HM%20355S%20Manual.pdf

Mike Andrews, W5EGO
WWME Oklahoma area executive team

 On Nov 10, 2014, at 11:11 AM, Stoll, Kathryn kst...@mcw.edu wrote:
 
 I have been given a Microm 335E microtome.  I do not have a user manual with 
 it.  Does anyone know how to turn the retraction on and off?
 
 Kathryn Stoll
 Supervisor Histology
 Clinical and Translational Research Core Lab Medical College of 
 Wisconsin
 9200 W. Wisconsin Ave Room 1176
 Milwaukee WI 53226
 Phone: 414-805-1525
 Fax: 

[Histonet] RE: CPT Code changes

[Rathborne, Toni]

Where can we find more information on this?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Monday, November 10, 2014 4:39 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CPT Code changes

Have you all seen this news?


To eliminate these discrepancies, CPT(r) 2015 revises parent code 88342, 
imunohistochemistry or immunocytochemistry, per specimen; initial single 
antibody stain procedure, deletes indented code 88343, and adds two new 
indented codes, +88341, each additional single antibody stain procedure, and 
88344, each multiplex antibody stain procedure.


Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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[Histonet] RE: Cold plates for icing blocks?

[Rathborne, Toni]

I had an employer years ago who used this. The disadvantage that bothered me 
the most was the paraffin shavings flying around due to lack of moisture. It 
created a very dirty environment, and there were frequent floaters due to the 
difficulty in keeping the microtome blade clean. I wouldn't recommend this at 
all, but maybe your conditions have more humidity and could work with this.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Monday, November 03, 2014 2:29 PM
To: Histonet
Subject: [Histonet] Cold plates for icing blocks?

Does anyone use a cold plate, like that used for embedding, for icing blocks 
for sectioning? Just an idea

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC 
San Francisco Medical Center Box 1656
505 Parnassus Ave
San Francisco, CA 94143
USA

415.514-6042  (office)
tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org

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[Histonet] RE: slides for IHC's

[Rathborne, Toni]

We have a Bond III, and although we use the Apex slides from Leica, we are not 
required to do so. We have 3 different manufacturer's slides validated should 
the Apex slides go on backorder.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Johnson
Sent: Thursday, October 30, 2014 12:38 PM
To: 'Goins, Tresa'; Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: slides for IHC's

What type of instrumentation does everyone use?  We use Ventana Benchmark Ultra 
and they want us to use Superfrost Plus slides only...curious if other have 
the same restrictions

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa
Sent: Wednesday, October 29, 2014 1:53 PM
To: Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: slides for IHC's

Mercedes Medical Starfrost adhesive for IHC and HE

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen 
- SMMC-SF
Sent: Wednesday, October 29, 2014 11:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] slides for IHC's

I would like to know what kind of slides everyone else is using for their 
IHC's.  I am having some problems with tissue falling off the slides during 
Target Retrieval.  This happens mostly on fattier tissue.

Thanks,

Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckf...@dignityhealth.org

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[Histonet] RE: slides for IHC's

[Rathborne, Toni]

Leica Apex slides.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa
Sent: Wednesday, October 29, 2014 2:53 PM
To: Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: slides for IHC's

Mercedes Medical Starfrost adhesive for IHC and HE

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen 
- SMMC-SF
Sent: Wednesday, October 29, 2014 11:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] slides for IHC's

I would like to know what kind of slides everyone else is using for their 
IHC's.  I am having some problems with tissue falling off the slides during 
Target Retrieval.  This happens mostly on fattier tissue.

Thanks,

Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckf...@dignityhealth.org

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[Histonet] RE: Working on Saturday

[Rathborne, Toni]

Since ANP 22983 has been changed to state that breast fixation in formalin is 
between 6-72 hours, we no longer have staff come in on weekends. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Thursday, October 23, 2014 1:44 PM
To: 'Smallwood, Lorraine'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Working on Saturday

We stopped Sat a few years ago. We have someone on call if something is needed. 
We trained the clinical lab to take the breast cases off the processor so we 
rarely need to come in.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Smallwood, 
Lorraine
Sent: Thursday, October 23, 2014 1:28 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Working on Saturday

We are a 450 bed hospital.  Due to our size and volume of work, our work week 
is Monday-Saturday. Because of healthcare reform, budget cuts and staffing 
shortage, we are faced with closing the department on Saturday (!!Histotechs 
are happy!!). One tech will continue to come in on Saturday to process any 
breast specimens received.   I am curious to find out what is the norm 
concerning working on Saturdays.  How many other Histology departments work on 
Saturday?  Thanks in advance for your opinions!

Lorraine Smallwood MScEd, PA(ASCP)
BB(ASCP)IHC, MLS(ASCP)HT, MT(AMT)
Pathologist Assistant


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[Histonet] RE: H Pylori IHC

[Rathborne, Toni]

You might be able to get around this by doing the IHC on all of these as 
requested, but only charging for those which are indicated as being medically 
necessary. This could benefit your TAT, if that's the reason the pathologist 
wants these done routinely. The pathologist would also have to agree not to 
bill for the PC of this test in these cases also. You might want to do a 
retrospective study and determine if this was done what the financial impact 
would have been.
Good luck.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
wanda.sm...@hcahealthcare.com
Sent: Monday, October 13, 2014 2:49 PM
To: sbald...@mhhcc.org; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: H Pylori IHC

Kathy,
My understanding is CMS (Medicare) has ruled that h.Pylori stains cannot be a 
standing order.  The Pathologist must look at the HE's first and deem the 
stain is needed based on the inflammation.


WANDA G. SMITH, HTL(ASCP)HT 
Pathology Supervisor 
TRIDENT MEDICAL CENTER 
9330 Medical Plaza Drive 
Charleston, SC  29406 
843-847-4586 
843-847-4296 fax 

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Baldwin, Kathy
Sent: Monday, October 13, 2014 2:36 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] FW: H Pylori IHC



Hi all
My pathologist would like our techs to do H pylori IHC stains on all stomach, 
GE Junction and esophagus BX's.  What I was wondering, if there were any 
indication in Regulations or anything that we couldn't do this?  Any advice 
would be greatly appreciated :)

S Kathy Baldwin
Histology/Cytology Supervisor
PH. 812-996-0210, Fax 812-996-0232
sbald...@mhhcc.orgmailto:sbald...@mhhcc.org:)

Christ's healing mission of compassion empowers us to be for others through 
Quality and Excellence
Vision Statement:  We are committed to being the preferred health and wellness 
provider; transforming lives through faith based, compassionate care.


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[Histonet] RE: Surgical Pathology reports

[Rathborne, Toni]

Yes, more often than you may imagine. Unexpected findings are always called and 
documented. See ANP12175. Although not required, another reason is the courtesy 
extended by the pathologist to the physician. This may occur if a preliminary 
report is to be issued, or the final report not signed out while the 
pathologist waits for additional tests to be completed. The pathologist may 
call if he/she knows that the physician is especially interested in getting the 
results for patient care reasons, and wants to explain why there may be delays 
to the final report.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lake,Debbie
Sent: Tuesday, September 23, 2014 1:23 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Surgical Pathology reports

Do pathologists ever call pathology reports to a physician?  If so, what types 
of diagnoses do they call?  There are no critical test results in Pathology 
that CAP deems necessary to call as in other areas of the laboratory..  How do 
others handle this?


Debra Lake  MT(ASCP)
Manager Micro, Blood Bank, Pathology
Marion General Hospital
Marion, IN  46952
(765) 660-6521
Fax: (765-651-7330)






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[Histonet] RE: No food or drink in the lab

[Rathborne, Toni]

What is staff doing at their desks? Are the desks located in a work area where 
specimens or chemicals are? If not, and the desk area can be  clearly separated 
from the work area, then maybe  you could have a sign posted in each area. No 
food or drink permitted in this area or Food and drink permitted in this 
area.  This way it clearly states where the food will be allowed. If their 
desks are in an area where specimens are handled, then disciplinary action 
should follow.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Osborne, Dale
Sent: Thursday, September 04, 2014 9:55 AM
To: Wineman, Terra; Amber McKenzie
Cc: Histonet
Subject: [Histonet] RE: No food or drink in the lab

Hello,
   I've attached the policy that Washington University has regarding food and 
drink in the lab.  If found upon lab inspection, the university and/or 
department may be fined.

Dale Osborne
Washington University School of Medicine Anesthesiology Dept.
St. Louis, MO

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Wineman, Terra 
[terra.wine...@novusint.com]
Sent: Thursday, September 04, 2014 8:38 AM
To: Amber McKenzie
Cc: Histonet
Subject: [Histonet] RE: No food or drink in the lab

Pretty sure that is an OSHA regulation.

Terra Wineman, HTL (ASCP)CM
Research Biologist, Nutritional Physiology
636-926-7476 phone
terra.wine...@novusint.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Thursday, September 04, 2014 8:24 AM
Cc: Histonet
Subject: [Histonet] No food or drink in the lab

Does anyone know of a specific site and quote that states No food or drink in 
the lab and what the consequences would be?  That covers drinks/snacks in the 
cabinets above their workstations too, right? I have a sign up on the door 
entering the lab that states no food or drink, but the staff continue to keep 
stuff around their desk, so I need something to back me up from CLIA, OSHA, 
CAP, etc...

Thanks!




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[Histonet] RE: Cytokeratin AE1/AE3

[Rathborne, Toni]

Would you share the source for the  sentinel lymph node recommendation 
mentioned? Although I just did a quick search, I didn't see anything on the CAP 
website.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard
Sent: Thursday, July 10, 2014 4:08 PM
To: Burnett, Brandy; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Cytokeratin AE1/AE3

Extra-follicular reticulm cells contain cytokeratin and will label with 
antibodies to low molecular weight cytokeratin.  Could that be what you are 
seeing?

Reference:  Linden MD and Zarbo RJ:  Cytokeratin immunostaining patterns of 
benign, reactive lymph nodes:  applications for the evaluation of sentinel 
lymph node specimen.  Appl Immunohistochem Mol Morphol 2001;9(4):297-301.

On another note,  the recommendation now is to not do CK IHC on sentinel lymph 
nodes (except for rare cases).  Lymph node involvement should be based on HE; 
not IHC.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic 
Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 Office
(860) 545-2204 Fax
richard.car...@hhchealth.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy
Sent: Thursday, July 10, 2014 12:08 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Cytokeratin AE1/AE3

Hi Histonetters,

I just came across a thread regarding issues with Cytokeratin AE1/AE3 from 
DAKO. I am having the same issues with our New protocol using HIER on the 
Link-48 in which sentinel lymph nodes from breast cases are staining 
non-specifically in-between the cells.
We never had this issue before on our old Autostainer plus, however, we were 
using only enzyme pre-treatment on it before staining.
I was wondering if anyone else has come across this, and if they have fixed it?

Thank you,

Brandy Burnett, HTL
Cape Cod Hospital

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RE: [Histonet] Paraffin blocks

[Rathborne, Toni]

Ours are incinerated because of the PHI.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: Thursday, July 10, 2014 4:56 PM
To: Vincent Rivera; 'Cooper, Brian'; JOSEPH FRAZEE; histonet
Subject: RE: [Histonet] Paraffin blocks

I have been following this stream of conversation and thought I might jump in. 
Paraffin blocks and microtomy waste are NOT biohazard waste. The process of 
fixation and tissue processing makes the tissue acceptable for general waste 
disposal. You do have to address the issue of PHI on the blocks, if you are 
using bar  code information and especially if patient information is included 
in the bar code. All bar codes used in the lab can be read in any standard word 
or spreadsheet software. I do not see the need to pay for expensive removal of 
biohazard waste, use general waste disposal for your blocks and cutting waste.

William DeSalvo, BS HTL(ASCP)
 
 From: vriv...@westderm.com
 To: bcoo...@chla.usc.edu; jfra...@hotmail.com; 
 histonet@lists.utsouthwestern.edu
 Date: Thu, 10 Jul 2014 20:37:39 +
 Subject: RE: [Histonet] Paraffin blocks
 CC: 
 
 You would think, especially when you see the amount of waste a tech can 
 produce on high volume days, but we also discard ours into regular trash 
 too. Just curious how other facilities handle this type of waste.
 
 Regards,
 
 Vincent Rivera, HT (ASCP), QIHC, QLS
 Histopathology Supervisor
 West Dermatology Pathology Laboratory
 vriv...@westderm.com
 714-924-7240 (Lab)
 714-390-0906 (Cell)
 
 
 -Original Message-
 From: Cooper, Brian [mailto:bcoo...@chla.usc.edu] 
 Sent: Thursday, July 10, 2014 1:30 PM
 To: Vincent Rivera; JOSEPH FRAZEE; histonet server
 Subject: RE: [Histonet] Paraffin blocks
 
 Good question.  So the linear path would dictate that they too should be 
 discarded into red bags, right?   You could also pose the same question about 
 the kimwipes used to skim your flotation baths.  We've always just thrown 
 both the shavings and kimwipes into the regular trash though.Guess we're 
 not so linear . . . 
 
 Thanks, 
 
 Brian
 
 
 -Original Message-
 From: Vincent Rivera [mailto:vriv...@westderm.com]
 Sent: Thursday, July 10, 2014 1:12 PM
 To: Cooper, Brian; JOSEPH FRAZEE; histonet server
 Subject: RE: [Histonet] Paraffin blocks
 
 I know this is slightly off topic but still along the same lines. How is 
 everybody handling their paraffin shaving waste? The waste that is produced 
 when trimming/facing tissue blocks? Is this also considered regulated medical 
 waste?
 
 Thanks,
 
 Vincent Rivera, HT (ASCP), QIHC, QLS
 Histopathology Supervisor
 West Dermatology Pathology Laboratory
 vriv...@westderm.com
 714-924-7240 (Lab)
 714-390-0906 (Cell)
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian
 Sent: Thursday, July 10, 2014 12:23 PM
 To: JOSEPH FRAZEE; histonet server
 Subject: RE: [Histonet] Paraffin blocks
 
 In every institution I've ever worked, paraffin tissue blocks were handled as 
 regulated medical waste, and therefore discarded into red biohazard bags.
 
 Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology 
 and Laboratory Medicine Children's Hospital Los Angeles bcoo...@chla.usc.edu 
 
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of JOSEPH FRAZEE
 Sent: Thursday, July 10, 2014 12:13 PM
 To: histonet server
 Subject: [Histonet] Paraffin blocks
 
 How is everyone disposing of paraffin blocks regular trash or biohazard 
 containers
 
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RE: [Histonet] Eosin Leaching

[Rathborne, Toni]

Is your alcohol level as high as or higher than the level of eosin in the 
containers? If there is residual eosin left at the upper portion of the slide, 
that could be causing the problem.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, 
Jeanine (CDC/OID/NCEZID)
Sent: Thursday, June 19, 2014 5:26 PM
To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu'
Subject: RE: [Histonet] Eosin Leaching

Have you changed your coverslipping mountant?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne 
Anderson
Sent: Thursday, June 19, 2014 5:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin Leaching

Hello all,

We're having a problem with our eosin bleeding out of the sections. I've read 
that this can be caused by not dehydrating adequately after eosin, but we're 
still having an issue when using fresh alcohols. Does anyone have any other 
ideas as to the cause of this problem?

Thanks in advance for your help!

Adrienne



Adrienne Anderson, BS, HTL(ASCP)
Histotechnologist
Phylogeny, Inc. 
1476 Manning Pkwy, Powell, Ohio 43605
Phone: (614) 846-6161 
Fax:  (877) 591-1815

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[Histonet] RE: specimen release

[Rathborne, Toni]

Correction! My first response did not accept the copy/paste strikethroughs.





NOTE: Waste disposal must be in accord with all regulations and disposed of 
with minimum danger.

Specimens returned to professional, technical, and custodial personnel patients 
(e.g. prostheses, gallstones) or otherwise released to others (e.g. pacemaker 
or prosthesis to manufacturer) must be disinfected before release.



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Monday, May 05, 2014 4:35 PM
To: 'Cartun, Richard'; Gale Limron; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: specimen release



You may want to consider the CAP checklist which has recently been revised.



**REVISED** 04/21/2014

ANP.24200

Biohazard Waste Disposal

   Phase II

Infectious tissues and other potentially contaminated materials are disposed of 
with minimum danger to professional, technical, and custodial personnel, and to 
recipients.



NOTE: Waste disposal must be in accord with all regulations and disposed of 
with minimum danger.



Specimens returned to professional, technical, and custodial personnel patients 
(e.g. prostheses, gallstones) or otherwise released to others (e.g. pacemaker 
or prosthesis to manufacturer) must be disinfected before release.



-Original Message-

From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard

Sent: Monday, May 05, 2014 3:57 PM

To: Gale Limron; histonet@lists.utsouthwestern.edu

Subject: [Histonet] RE: specimen release



In the state of Connecticut we can only release human tissue to a funeral home. 
 Other than a hospital or a laboratory, a funeral home is the only company 
licensed to handle human tissue.  The patient then deals with the funeral home.



Richard



Richard W. Cartun, MS, PhD

Director, Histology  Immunopathology

Director, Biospecimen Collection Programs Assistant Director, Anatomic 
Pathology Hartford Hospital

80 Seymour Street

Hartford, CT  06102

(860) 972-1596 Office

(860) 545-2204 Fax

richard.car...@hhchealth.orgmailto:richard.car...@hhchealth.orgmailto:richard.car...@hhchealth.org%3cmailto:richard.car...@hhchealth.org



-Original Message-

From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gale Limron

Sent: Monday, May 05, 2014 12:38 PM

To: histonet@lists.utsouthwestern.edu

Subject: [Histonet] specimen release



We are looking into revising our policy/release form regarding the release of 
specimens to patients and I am interested in knowing what others are doing.

Thanks,

Gale



Gale Limron HT,CT (ASCP)

Histology Supervisor

Union Hospital

659 Boulevard

Dover, Ohio 44622

330-343-3311 ext 2562







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[Histonet] RE: Pathology Report Templates

[Rathborne, Toni]

Can you cite a source for the MU requirement?


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin
Sent: Friday, April 25, 2014 11:25 AM
To: 'Mike Pence'; 'Victor A. Tobias'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Pathology Report Templates


This is for the MU (meaningful use)
-Original Message-
From: Mike Pence [mailto:mpe...@grhs.net]
Sent: Friday, April 25, 2014 6:00 AM
To: Jesus Ellin; 'Victor A. Tobias'; histonet@lists.utsouthwestern.edu
Subject: RE: Pathology Report Templates

I went through all my CAP check list questions and I do not see this anywhere.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin
Sent: Thursday, April 24, 2014 12:54 PM
To: 'Victor A. Tobias'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Pathology Report Templates

This is a fact, to meet compliance the signing facility needs to be ID along 
with the CLIA#, ,haven't heard about the medical director issue, but who knows

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias
Sent: Thursday, April 24, 2014 10:51 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Pathology Report Templates

One of our facilities had a CAP inspection last week and I was asked to add the 
CLIA# and Medical Director to the template, immediately. Is this something new, 
as we had our CAP inspection last Aug. and there was no mention about CLIA# or 
having the Director's name on the templates?

I haven't been able to get any follow-up from the lab supervisor.

Victor Tobias HT(ASCP)
Clinical Applications Analyst
Harborview Medical Center
Dept of Pathology Room NJB244
Seattle, WA 98104
vtob...@u.washington.edumailto:vtob...@u.washington.edu
206-744-2735
206-744-8240 Fax
=
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RE: [Histonet] RE: competency form

[Rathborne, Toni]

CAP inspectors may have opinions which differ from our own, and their 
interpretation of standards may also be different. Have you challenged this 
deficiency with CAP? 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas
Sent: Wednesday, April 09, 2014 8:52 AM
To: joelle weaver; Amber McKenzie; Houston, Ronald; Histonet
Subject: RE: [Histonet] RE: competency form

What we had here, which did not meet the CAP inspectors requirements 
apparently, were the procedure (of course); a form with each observation of 
each task documented along with any corrective action necessary; the 
correlation of proficiency tests, educational assessments  and performance 
reviews for technical staff; daily evaluations from the pathologists concerning 
staining, microtomy and grossing; and educational training documentation. We 
had no idea with all pieces of this documentation that we were anticipated to 
have more. The form for DO of each task was not detailed enough (despite 
listing each task and proficiency or corrective action of each task) according 
to the inspectors. I was hoping someone could share a form so as to assist us 
in seeing what holes there are in our form. While this DO form is definitely 
not our only form, the inspectors specifically commented on this one.
 
If anyone can assist, it would be helpful.
Thank you,
Sheila Haas
MicroPath Laboratories, Inc.
Quality Assurance Coordinator

 
From: joellewea...@hotmail.com
To: sm...@msn.com; amber.mcken...@gastrodocs.net; 
ronald.hous...@nationwidechildrens.org; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: competency form
Date: Wed, 9 Apr 2014 12:26:47 +




 
Is the question that Ronnie posed yesterday requesting justification of the 
need and extent of competency documentation for histology, or is it just a form 
needed? The general checklist pretty much sums up the necessity for doing, and 
required elements to me- GEN. 55500 and CLIA. Maybe I am not understanding?
As for a form, I would expect that the specific items on any forms will vary by 
your personnel and by the testing and processes you perform. 
What I did to document initial training and competency was a make a summary 
checklist for each bench with tasks and direct observations DO for initial 
training   documentation of satisfactory performance before patient testing.
I just put all those checklists together in a summary table for each person. 
High complexity; such as grossing, IHC, FISH scoring get more attention and 
documentation, the waived tests, you have more discretion,- but I thought it 
easier to do everything about the same. I have not been inspected on this 
document yet ( so can't say if CAP will have issues with it- but will know 
soon...) but here is basically what I did to meet GEN.55500 or the main parts; 
 
 
Defined how competency is monitored- method and frequency ( just included as 
part of the competency SOP) Orientation and initial training documentation in a 
checklist for general lab, safety Training checklist on each technical bench, 
instrument, major procedure PT records and performance/results
DO- a practical assessment ( block, slides, stains),  for the assessment of 
previously analyzed specimens, and a PI feedback checklist for the technical 
from this audit of issues- how/what to improve Check off in performing QC, 
calibration, patient ID procedures ( acceptable error rates), examples for file 
DO of grossing, other performance such as instrument programming/maintenance 
Written quiz, policies  procedures, troubleshooting( problem solving 
documentation) Continuing education participation records





Joelle Weaver MAOM, HTL (ASCP) QIHC
 
 From: sm...@msn.com
 To: amber.mcken...@gastrodocs.net; 
 ronald.hous...@nationwidechildrens.org; 
 histonet@lists.utsouthwestern.edu
 Date: Wed, 9 Apr 2014 11:10:18 +
 Subject: RE: [Histonet] RE: competency form
 CC: 
 
 We were recently dinged by CAP for our competency assessments in all areas. 
 While that's no longer my immediate responsibility, I would love to be able 
 to assist the lab manager with some information so we can tweek our 
 assessments if you all wouldn't mind sharing with me as well.
 Thanks a bunch.
 Sheila Haas
 MicroPath Laboratories, Inc.
 Quality Assurance Coordinator
  
  From: amber.mcken...@gastrodocs.net
  To: ronald.hous...@nationwidechildrens.org; 
  histonet@lists.utsouthwestern.edu
  Date: Tue, 8 Apr 2014 21:17:44 +
  CC: 
  Subject: [Histonet] RE: competency form
  
  Can you pass it on to me as well?  I'd love to compare what I've got to 
  what someone else is doing. 
  Thanks,
  Amber
  
  -Original Message-
  From: histonet-boun...@lists.utsouthwestern.edu 
  [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
  Houston, Ronald
  Sent: Tuesday, April 08, 2014 2:37 PM
  To: Histonet
  Subject: [Histonet] competency form
  
  Can someone 

[Histonet] RE: Body Release and Cleanin in Morgue

[Rathborne, Toni]

Security is responsible for the release of bodies to funeral homes, and all 
staff must contact Security to access the morgue. We have found that to be the 
best way to avoid being questioned about anything. We have stretchers here not 
drawers, so I can't respond to that.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
Sent: Wednesday, March 26, 2014 5:11 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Body Release and Cleanin in Morgue

Hello to all in histo-land.  Who takes care of releasing the body to the 
funeral home and who does the cleaning of the seals and the molds on the 
drawers for the body storage?  Is lab some how involved in this process?


Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-2148
Lab: 713-566-5287


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[Histonet] RE: Pathology Protocols

[Rathborne, Toni]

Joe,
I'm just curious about handwritten notes. We also have Cerner Millennium, but 
when I tried a few years ago to find out if there would be an impact in any of 
the areas, the OR reminded me that some of the surgeons like to draw diagrams 
for margins. The pathologists also document on the paper requisition when and 
who was called with frozen results. Although this is a small percentage of the 
specimens we receive, it stalled the project. How do you get around this?
Toni

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe W. Walker, 
Jr.
Sent: Tuesday, March 11, 2014 12:21 PM
To: Bauer, Karen L.; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Pathology Protocols

Hi Karen,

We utilize Cerner Millennium at my institution.  We are 90% paper free.  
Fortunately for us, the vast majority of our surg path orders come to us as an 
electronic order, either placed by the provider on the floor or through an 
integrated office EMR.  All op notes, office notes, radiology, etc are also 
available electronically to the pathologist at the time of the gross dictation. 
 The gross dictation is transcribed into the surg path report prior to the 
pathologist getting their slides the next day.  The pathologist uses either a 
bar code to scan the slide/accession number to bring up the surg path report 
with the transcribed gross dictation.  The pathologist can again access all of 
the op notes, etc at the time of the final report's dictation. Also, all 
clinical test results are available within the application the pathologist uses 
to view the reports.  Our transcriptionist manages the movement of cases to and 
from the pathologists' queue for their verification.

The 10% of paper requisitions that we receive are ordered by our administrative 
staff where all of the written information on the paper is transferred to the 
electronic order.  The paper reqs are then scanned into our system.  Once in 
our system, the process for the paper reports is the same as the above.  The 
exception is that we generally don't have office notes when we receive orders 
on paper.

A couple of our pathologist do utilize a dual monitor set up that allows them 
to have the surg path report and the clinical info like radiology on a 
different monitor for their comparison.  It didn't take a lot of convincing to 
get rid of the paper and has helped improve the accuracy of the orders and 
diagnostic information.  There were definitely adjustments to everyone's 
workflows but we have been operating this way for 2 years now with very few 
problems.

If you have the capability, I'd highly encourage removing the paper process,

Joe W. Walker, Jr. MS, SCT(ASCP)CM
Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional 
Medical Center
160 Allen Street, Rutland, VT 05701
P: 802.747.1790  F: 802.747.6525
Email joewal...@rrmc.orgwww.rrmc.org

Our Vision:
To be the Best Community Healthcare System in New England

Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet 
Recognition(r) and the Governor's Award for Performance Excellence


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L.
Sent: Tuesday, March 11, 2014 11:25 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Pathology Protocols
Importance: Low

Hi all,

Are there any AP labs that are totally paper free during slide diagnosis?

We have the Vantage system with Sunquest CoPath.  We still keep the paper 
specimen requisition with the specimen container during grossing.  After 
digital gross dictation, the req slips are given to the transcriptionists.  
Transcriptionists type up the gross, print out patient histories, and place 
dictation and histories in a plastic sleeve.  These case protocols are then 
brought back to Histology to be matched up with the slides.

Slides are place in cardboard trays and matched up with the protocols.  These 
are then placed in the pathologist slide area for the docs to pick up.

We would really like to get rid of the paper protocols.  Having Vantage, docs 
are able to scan the slides at their desks to bring up the patient information. 
 Unfortunately, we are not doing this at this time.

If there are any labs who are doing this, could you please tell me how your 
computer systems are set up?  Are the specimen reqs scanned at accessioning?  
Do the docs have two monitors at their desks so they can view gross dictation 
and patient history at the same time?  What did you do to essentially get rid 
of all the paper information?

Any information that anyone can share with me is greatly appreciated.  :)

Thank you,

Karen

Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab 
Supervisor | Dermatology | Phone: 715-838-3205 | 
bauer.ka...@mayo.edumailto:bauer.ka...@mayo.edu | Mayo Clinic 

[Histonet] Slides for control/patient

[Rathborne, Toni]

Happy Friday!
I was wondering, for the labs that are using the slides for IHC that have a 
place for the control tissue and the patient tissue, which vendor would you 
recommend. I also would not mind being contacted directly by vendors who wish 
to inform me of their products.

Thanks,
Toni
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RE: [Histonet] RE: Ventana LEAN

[Rathborne, Toni]

Another benefit of someone coming in from the outside (Leica did the same for 
us), is that since this is what these people do every day, management is more 
likely to listen to their suggestions. If it's the same as yours then that 
would reinforce your current plans. If it's different than what you had in 
mind, they will generally try to help with what you want to do.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Higgins
Sent: Monday, February 03, 2014 8:59 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Ventana LEAN

They come in, give their opinions on how to make your workflow better.  I 
didn't get much from it, we didn't make any changes, more of a selling tool in 
my opion.  I guess if your workflow was terrible, they might be able to offer 
good advice.
 
On a positive, they were all very nice.
 
Thank,
Tim H.
 
  
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[Histonet] RE: Frozen sections

[Rathborne, Toni]

Our pathologists prefer to perform all aspects of frozen section preparation. 
We will stain for them, but they would rather gross and section themselves.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Leann M. Murphy
Sent: Monday, January 13, 2014 10:35 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Frozen sections

Good morning,

I know all histotechs help cut and stain frozen sections.  Is there any 
organization  out there that has the histotechnician gross the frozen section 
tissue and place on the frozen section chuck to cut without the Pathologist in 
the room.  If anyone does this please tell me why?  And if your organization 
doesn't do this please tell me why.  I am having a debate with our Pathologists 
over this question.

Thank You,
LeAnn Murphy
Aultman Hospital
Canton, Ohio



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[Histonet] RE: Safety items

[Rathborne, Toni]

Safety glasses, counter top shield, lab coats. A fire blanket is another safety 
measure you could look into. Be sure that sprinklers are tested periodically, 
and that all flammables are in a safety cabinet.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Tuesday, January 07, 2014 9:35 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Safety items


What safety items do you keep in your lab besides the normal apron, gloves, eye 
wash stations and shower?  Thanks!

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RE: [Histonet] Safety items

[Rathborne, Toni]

Are they looking for documentation that it's been checked, or will they take 
your word for it?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eytalis, Robert 
A
Sent: Tuesday, January 07, 2014 10:51 AM
To: Anne Murvosh; Amber McKenzie; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Safety items

Newest CAP inspection stupid thing. They want you to periodically check your 
spill kit for clumping, since it does not have an expiration date. I challenged 
it, but don't know if will go through. 

Robert A. Eytalis
Laboratory Manager
robert-eyta...@riversidehealthcare.net
Phone: (815) 935-7256 ext. 5186
(815) 935-7535
Fax (815) 935-7068

Riverside Medical Center
350 N. Wall Street - Kankakee, IL 60901


http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.URL=http%3a%2f%2fwww.riversidemc.net%2f
  |  
http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.URL=http%3a%2f%2fwww.facebook.com%2fRiversideMC



From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Anne Murvosh 
[amurv...@advancederm.net]
Sent: Tuesday, January 07, 2014 9:25 AM
To: Amber McKenzie; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Safety items

Don't forget to make sure you have a spill kit and its labeled well with easy 
access,  Inspectors will ask employees if they know where it and your fire 
extinguisher and alarm are located. Anne

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Tuesday, January 07, 2014 6:35 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Safety items


What safety items do you keep in your lab besides the normal apron, gloves, eye 
wash stations and shower?  Thanks!

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RE: [Histonet] RE: Amended Reports

[Rathborne, Toni]

I really like the idea of a waiver/re-label form. Other than approval from the 
Laboratory Director, did you have to get approval from any other departments to 
put this form into use?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Tuesday, December 03, 2013 1:43 PM
To: Laurie Colbert; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Amended Reports

This is just one method ( manual right now-no automation or barcoding- which I 
feel helps).
 But anyhow the SOP is that the name/specimen information has to be cross 
checked at each pre-analytic step ( transport manifest, accessioning, grossing, 
embedding, microtomy, stained slides for example). 
Each person has responsibility to stop/hold a specimen for an issue at any 
task. Identification or label discrepancies noticed at accessioning or 
grossing, are called to the submitting facility/clinician who is responsible to 
send someone when possible to re-label/identify. They sign a waiver/re-label 
form taking responsibility for the accuracy of the relabeled information.Do not 
normally send the specimen back, or normally reject due to the irreplaceable 
nature of many specimens, but if anything is amiss, this is documented at each 
check point.  A copy of the re-label form is retained with the specimen 
requisition that travels to the Pathologist.  The final call to reject or send 
back any really mis-identified specimen is at the discretion of the 
pathologist. 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
 From: lcolb...@pathmdlabs.com
 To: ll...@aipathology.com; histonet@lists.utsouthwestern.edu
 Date: Tue, 3 Dec 2013 17:09:06 +
 CC: 
 Subject: [Histonet] RE: Amended Reports
 
 The specimen container and requisition should always be compared before 
 accessioning.  If the name on the specimen container label and the name on 
 the requisition do not match, we do not accept the specimen.  It should be 
 sent back to be properly labeled.
 
 Laurie Colbert, HT (ASCP)
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of LeAnn Lang
 Sent: Tuesday, December 03, 2013 7:42 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Amended Reports
 
 I need your help with the following situation.  
 
  
 
 We receive specimens from an outside hospital and the specimen request slip 
 and specimen container are given the accession number at that location.  Once 
 it is received in our laboratory, we process the specimen.  A situation came 
 up recently where the outside hospital mixed up the paperwork on three cases 
 (so the wrong request slip was placed with the wrong specimen container, but 
 it was not caught in our laboratory because they did not all come together).  
 Anyhow, long story short, the original reports got reported as:
 
  
 
 The specimen request slip is labeled as Joe Smith and the specimen 
 container is labeled as Jane Doe.  The specimen consists of
 
  
 
  
 
 After the reports were finalized, and sent back to the clinician, the mistake 
 was identified.  We made amended reports on our end to correct everything per 
 CAP guidelines.  The problem we are now running into is that the Medical 
 Records dept is telling us that we are in HIPAA violation by having the two 
 patient names on the report.  I see their point, but don't know any other way 
 of doing this.  
 
  
 
 Anyone else ever deal with a situation like this?  If so, how do you handle 
 it?
 
  
 
 Thank you!
 
 LeAnn
 
  
 
 **
 
 LeAnn Lang
 
 Associates in Pathology
 
 Practice Administrator
 
 Phone:  715-847-0075 (ext 50259)
 
 ll...@aipathology.com mailto:ll...@aipathology.com 
 
  
 
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RE: [Histonet] Specimens from surigical units

[Rathborne, Toni]

We have a book for each area that sends us specimens. A patient label is placed 
in the book by the collecting location, with the number of containers 
collected. The person who places the labels/specimen in the bin will initial 
the book. When the specimen is received(either picked up or delivered), the 
person getting the specimen initials after matching the correct number of 
containers and the patient information. The books are housed in the sender's 
location, and any discrepancies can be reviewed afterwards. We also have a 
pending log that prints at the end of the day which we check. If something 
should have arrived and hasn't, we will call to find out if there was actually 
a specimen collected.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Wednesday, November 20, 2013 12:10 PM
To: 'WILLIAM DESALVO'; Vickroy, Jim; histonet
Subject: RE: [Histonet] Specimens from surigical units

We have a similar system.  When pathology specimens are taken in surgery a 
notification is generated and sent to our printer.  We reconcile the 
notifications with the requisitions when the specimens are received.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas 
Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: Wednesday, November 20, 2013 10:09 AM
To: Vickroy, Jim; histonet
Subject: RE: [Histonet] Specimens from surigical units

We use the hospital LIS. All specimens/cases are entered and ordered by Surgery 
and they are then received by Surgical Pathology. You are able to effectively 
track w/ pending lists and a full manifest/reconciliation process.

William DeSalvo, BS HTL(ASCP)

 From: vickroy@mhsil.com
 To: histonet@lists.utsouthwestern.edu
 Date: Wed, 20 Nov 2013 10:03:45 -0600
 Subject: [Histonet] Specimens from surigical units
 
 
 We are a large pathology department and process over 35,000 surgical 
 accessions each year.  I am interested in learning how other pathology 
 departments keep track of the specimens that are removed in surgery to make 
 sure they are received in pathology.  Obviously all specimens received by 
 pathology are entered into the pathology accessioning system however the 
 question has come up to how do we know that every specimen taken in surgery 
 for pathology is actually received in pathology.  A couple of our surgical 
 units utilize volunteer couriers since the surgical units are not near 
 pathology.   A couple of other units like special procedures and cardiac 
 surgery have a simple notebook that is a signoff when the specimens are taken 
 to pathology, however from there is no tracking system from the main OR or 
 outpatient surgery. In otherwords a specimen could be removed in the OR that 
 was supposed to be sent to pathology does not arrive in pathology.  Obviously 
 this doesn't happen often but we are trying to close the potential for an 
 occurrence.   Specimens removed in surgery are logged into the surgical OR 
 report however  there is no current way for us to track that everything 
 removed that day that was supposed to go to pathology actually made it to 
 pathology.   Any ideas?
 
 Jim Vickroy
 
 James Vickroy BS, HT(ASCP)
 
 Surgical  and Autopsy Pathology Technical Supervisor Memorial Medical 
 Center
 217-788-4046
 
 
 
 This message (including any attachments) contains confidential information 
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RE: [Histonet] Specimen collection/transportation

[Rathborne, Toni]

Did they state which OSHA standard you were in violation of? 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Will Chappell
Sent: Monday, November 18, 2013 3:06 PM
To: LeAnn Lang
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Specimen collection/transportation

This to me seems very odd. Almost exclusively specimens are sent to my lab in 
formalin. Placentas are usually sent fresh simply because of their size. 

If anything, the birthing unit may not be in compliance, but it has nothing to 
do with the lab. The formalin containers must be properly labelled, and 
appropriate SOPs in use on the floor, usually to include a spill kit. I wrote 
the procedures for the floor units, but it is their responsibility to be in 
compliance. 

Will Chappell, HTL(ASCP)

Sent from my iPhone

 On Nov 18, 2013, at 12:02 PM, LeAnn Lang ll...@aipathology.com wrote:
 
 We were recently contacted by our hospital indicating that we are in 
 violation of OSHA by using the process we currently are using.
 Currently, we provided prefilled 10% neutral buffered formalin
 containers to the surgical suites, birthing units, etc.   They fill the
 containers with the specimens and return them to the pathology lab.   We
 have done this process for many many years and have never been 
 questioned for this by either CAP or Joint Commission.  What is your 
 process for specimen collection/transport?  Are the specimens put in 
 formalin in the surgery suites/birthing unit/etc or in the pathology 
 laboratory?  How about placentas, are they sent in formalin from the 
 floor or are they put in formalin in the histology lab?
 
 
 
 Thank you!
 
 LeAnn
 
 
 
 
 
 **
 
 LeAnn Lang
 
 Associates in Pathology
 
 Practice Administrator
 
 Phone:  715-847-0075 (ext 50259)
 
 ll...@aipathology.com mailto:ll...@aipathology.com
 
 
 
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RE: [Histonet] (no subject)

[Rathborne, Toni]

I wouldn't be too quick to blame the blades. Have you changed paraffin 
brands/temperatures or changed any of your processing schedules? Is the 
fixative/fixation process the same? Is the collecting location doing anything 
different/have they had a change in staff? 
Also, what type of blades are you using? It would be easier for people to 
respond if they had this information.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Leann M. Murphy
Sent: Thursday, November 14, 2013 11:04 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

I was just wondering if anyone was having difficulty cutting biopsies?  We have 
been using the same blades for years and now it is so difficult to get a 
ribbon.  There are three other area hospitals having the same problem. I have 
been trying samples from various vendors and the problem is still the same.  
Maybe the company that makes these microtome blades have cut costs and is now 
using a lower grade of metal for the blade.  I don't  know what it is, but it 
is driving everyone crazy.  Also, we are spending more money on blades because 
they do not last as long and of course this does not make our Manager happy.  I 
am just very frustrated.  Any suggestions?

LeAnn Murph, HT (ASCP)
Aultman Hosptial
Canton, Ohio
Technical Specialist
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RE: [Histonet] Leica vs Ventana

[Rathborne, Toni]

Another suggestion would be to go through the Histonet archives to see what 
problems people have questioned/reported over the last year. Don't forget about 
the bulk waste either. When we had considered Ventana, the waste 
(hazardous/non-hazardous) was combined - it may not be now, I haven't checked 
lately. Be sure to check with your own local municipality with regard to 
disposal. This could end up being far more costly for your lab if you have to 
pay for the combined bulk disposal rather than just the separated hazardous 
waste.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Phyllis Burnette
Sent: Monday, November 11, 2013 5:13 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica vs Ventana

Our hospital facility, as well, are faithful Ventana customers.  We have 3 
ultras and appreciate the ease and flexibility needed for a busy hospital 
facility.  The slide drawers are random access so the slides are staining 
independently from one another.  Another great feature of the ultra is the 
random access points where antibodies, exhausted reagents, etc. can be added 
once the run starts and at multiple times during the staining run. Our 
laboratory has found that the quality of the IHC stains are exceptional and 
consistent from lot to lot.  The sales staff and technical support is superior 
and the engineers that work on our equipment are very thorough.  Of course, 
Ventana is light-years above with specimen tracking for the histology 
laboratory and we are very excited that with this addition, all slides will be 
tracked from start to finish.  Can't say enough, we really love this product!!!




Phyllis S. Burnette HT, ASCP
Anatomicals Supervisor
Riverside Regional Medical Center
757-594-2885
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RE: [Histonet] cryostat service

[Rathborne, Toni]

Avantik is located in NJ and services cryostats in addition to microtomes, 
processors and stainers.
www.avantik-us.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jean Ross
Sent: Monday, October 28, 2013 2:25 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cryostat service

Hi everyone,

I am interested in finding out if there is anyone in or close to Delaware that 
services and repairs cryostats.  Any recommendations would be much appreciated.

Thanks,
Jean
--
Jean Ross
Delaware Biotechnology Institute, BioImaging Center University of Delaware
15 Innovation Way
Suite 117
Newark, DE  19711
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[Histonet] RE: freezing spray

[Rathborne, Toni]

If they are hospital employees, you should have a little more say in what goes 
on in the lab. Is there a policy which addresses this? If not, now might be a 
good opportunity to draft one and have the Medical Director sign off on it. You 
can also get your Infection Control department involved if you need additional 
support.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Thursday, October 24, 2013 2:45 PM
To: histonet (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] freezing spray

Our hospital has not allowed freezing spray to be used in the frozen section 
lab for many years.  We now have a new group of doctors who want to use the 
spray.  The docs think the frozen sections take too long to freeze.  Yet, they 
meet the frozen section TAT for more than 98% of our cases.  I think it's worth 
not using it for the one case where no one suspects TB but the patient will be 
positive.

Do you allow freezing spray in your frozen section lab?


Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas 
Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.orgmailto:hor...@archildrens.org
archildrens.orghttp://www.archildrens.org/







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RE: [Histonet] HT HistoDeck question...

[Rathborne, Toni]

For those of you who use the 40% formaldehyde, how long is you fixation time on 
frozen slides? We use 10% NBF with 1 minute to fix, but sometimes it gets 
hectic if you have multiple frozens all at once. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer 
MacDonald
Sent: Thursday, October 03, 2013 2:04 PM
To: Grantham, Andrea L - (algranth)
Cc: HISTONET; histonet-boun...@lists.utsouthwestern.edu
Subject: Re: [Histonet] HT HistoDeck question...

We also use this oil red O method and use the 40% formaldehyde.
The questions lacks enough information to correctly answer it.  I am sure the 
author of the question had something in mind and other options didn't occur to 
him/her at the time.
Jennifer




From:   Grantham, Andrea L - (algranth) algra...@email.arizona.edu
To: 
Cc: HISTONET histonet@lists.utsouthwestern.edu
Date:   10/03/2013 08:33 AM
Subject:Re: [Histonet] HT HistoDeck question...
Sent by:histonet-boun...@lists.utsouthwestern.edu



I'd go with A, but it really depends on what you are going to do with the 
sections after fixation.

In the protocol for Oil Red O in Freida's second edition (that I use almost 
daily combined with some steps from PolyScientific's ORO protocol), step #1 
says to fix in 40% formaldehyde. Doesn't say vapors so I plunk the slides in 
the liquid 40%.
I have fixed frozen sections for regular HE and other stains as well in 40% 
formaldehyde and they come out beautiful.
Unless there is some specific request or reason to use cold acetone or alcohol 
this is what I use.

Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edumailto:algra...@email.arizona.edu
Tel: 520.626.4415 Fax: 520.626.2097





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RE: [Histonet] Lab Chairs

[Rathborne, Toni]

Do you have a link to where we could see them? 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roberta Horner
Sent: Friday, September 27, 2013 7:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Lab Chairs

I have 2 saddle chairs and I really like them.  You have to sit straight on 
them, no slouching.  I have less back aches since I got them.
Roberta Horner HT/HTL
Penn State University
Animal Diagnostic Lab
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[Histonet] Thermo Scientific HM 550 Cryostat

[Rathborne, Toni]

Looking for opinions on this cryostat. Has anyone used it? What is the Cold D 
like to use? How often is the solution replaced? What about the Vacutome? It 
sounds like a nice feature along with the Automatic Cryo-Approach.
Appreciate all responses.
Thanks,
Toni
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[Histonet] RE: Cerner Millennium

[Rathborne, Toni]

Have you tried asking on UCern? uCern Admin ad...@ucern.com
or
https://cernercare.com/accounts/login?returnTo=https%3A%2F%2Fconnect.ucern.com%2Fjiverp%2Fopenidendpoint.jspa%3Fprovider%3Dhttps%253A%252F%252Fcernercare.com%252Faccounts%252Fopenid%26destination%3D%252Femaildigestprofile.jspa#
uCern is a forum similar to histonet. You should be able to click on the link 
above and select preferences. I receive weekly updates, and have found some 
very good suggestions on there.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tracey Lenek
Sent: Wednesday, September 18, 2013 1:52 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Cerner Millennium

Hi,

We are a large multi-site facility and are in the midst of investigating the 
potential for Pathologists to order ancillary/additional testing in PTOE within 
our Millennium LIS.  As we have blocks at multiple locations and a large number 
of Pathologists, the list of ordering requests becomes quite large and 
difficult for our technical stream to manage.

Does anyone have any advice as to how to better manage the on line ordering 
process using Cerner Millennium?

Many thanks,
Tracey Lenek
Anatomic Pathology/Cytology Manager
Calgary Lab Services
403-770-3448



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RE: [Histonet] Flammable Liquid

[Rathborne, Toni]

Is this on the CLIA website? I agree that it's difficult to find anything on 
there. If you're able to provide a direct link that would be great!


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, September 17, 2013 11:56 AM
To: Anne Murvosh; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Flammable Liquid

The standard is 1 gallon per every 24 square feet of lab area, so if your lab 
has 240 square feet surface, you can store 10 gals of flammable liquids.
René J.



From: Anne Murvosh amurv...@advancederm.net
To: histonet@lists.utsouthwestern.edu 
Sent: Tuesday, September 17, 2013 11:23 AM
Subject: [Histonet] Flammable Liquid


Can anyone tell me how many gallons of Flammable liquid can be stored in
a space per CLIA regulations.  There website is useless.  Thanks Anne

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[Histonet] RE: Microtome Service

[Rathborne, Toni]

We also have Belair here in NJ. They have always done good work for us.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Walter Benton
Sent: Tuesday, September 10, 2013 7:25 AM
To: Fawn Bomar; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Microtome Service

http://www.dlrepair.com/index.php
http://dolbeyjamison.com/
http://belairinc.com/

Try any of these companies. I have used them all and they do quality work.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar 
[fawn.bo...@halifaxregional.com]
Sent: Tuesday, September 10, 2013 6:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtome Service

Does anyone know of any company that is able to service the Leica RM2155 
microtomes?  We are located in South Boston, Va.



Thank You



Fawn Bomar
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RE: [Histonet] GI biopsy slides

[Rathborne, Toni]

We've been doing this for years. You'd be surprised with the overall savings 
(slides, frequency of changing stains, storage), and also TAT since you'll be 
able to put through more cases on your stainer.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth 
Chatfield
Sent: Tuesday, September 10, 2013 11:32 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] GI biopsy slides

Hi folks,
 
We are re-thinking how we cut our GI biopsy slides. Currently we are cutting 3 
levels with 3 sections on each slide.  Is anyone out there putting all 3 levels 
on the same slide?  We are receiving cases with a large number of samples and 
some our pathologists would like to see fewer slides.  
 
 
Thanks,
Elizabeth
Charlottetown, PE

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RE: [Histonet] Cell Block Preparation

[Rathborne, Toni]

I wonder if this method could be used with the product Histogel. Has anyone 
tried it?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Sent: Friday, September 06, 2013 5:46 AM
To: 'Ann Specian'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Cell Block Preparation

This is how we do it now.  In the old days, we used agar and to my mind, it is 
still the best way when you have scant material.
- Spin in a conical tube and pour off
- Melt an agar slant (we get TSA slant from micro)
- Pour the agar into the conical tube and spin for 5 minutes
- The agar will re-solidify and whatever sediment there is will be concentrated 
in the very tip of the cone
- The agar will slide out of the centrifuge tube
- Slice off the very tip and wrap in lens paper
- Place the wrapped tip in a cassette and process as usual
- Embed the specimen tip down and you are good to go...

I still use this method today when I feel it necessary.  Works great.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian
Sent: Thursday, September 05, 2013 12:45 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cell Block Preparation


I am getting complaints in regard to insufficient cell blocks.  We currently 
spin, pour off the supernatant, retrieve the sediment and process in lens paper.

Does anyone have a more current technique which renders better cellularity?

Also, do you know which renders a better cell block:  a fresh specimen, a 
specimen fixed in Cytolyt or a specimen fixed in 10% NBF?

Thanks,
Ann
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accept liability for any errors or omissions in the contents of this message, 
which arise as a result of e-mail transmission. Thank you.

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RE: [Histonet] Isopropyl Alcohol in the histology lab

[Rathborne, Toni]

Rene,
You stated that immunoreactivity is not weakened by the use of IPA. Do you know 
how the use of IPA might affect FISH results? 
Toni
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, August 13, 2013 1:38 PM
To: Teri Johnson; 'gu.l...@gmx.at'
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Isopropyl Alcohol in the histology lab

Teri:
The automatic coverslipper will wok on oven dried stained sections. I used them 
on a Sakura film coverslipper and my lab was in Miami Beach, and you do not 
more humid than that!
Xylene is the one weakening the immunoreactivity the most but I have tested the 
IPA and the weakening does not exist although there are so many antibodies that 
some may weaken the issue is: will the weakening affect the diagnosis or just 
will produce a weaker reaction? That you would have to test further.
But the bottom line is tha xylene should be eliminated.
René  J.



From: Teri Johnson tjohn...@gnf.org
To: 'Rene J Buesa' rjbu...@yahoo.com; 'gu.l...@gmx.at' gu.l...@gmx.at 
Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
Sent: Tuesday, August 13, 2013 12:19 PM
Subject: RE: [Histonet] Isopropyl Alcohol in the histology lab



Thank you Rene. You raise many good points.
 
I have entertained the idea of eliminating xylene and believe it can be done 
successfully. It’s on my list of possible safety-related upgrades to the lab. I 
would very much like to get an automatic coverslipper in the lab, but don’t 
think that could work in a xylene-free environment. It might work using a 
substitute, but when the humidity in the lab gets high (we are a coastal city), 
it can overwhelm the aliphatic hydrocarbon.
 
I respectfully disagree with your assertion that immune reactivity is not 
further affected by processing after fixation. It is well known that alcohol 
and xylene can cause loss of immunoreactivity of proteins post-fixation. I 
think Bill Grizzle among others did some experiments and came to this 
conclusion. We tend to use antibodies and develop detection protocols that can 
work despite those factors. What concerns me is if we change the alcohol, will 
that increase or decrease any immunoreactivity that might cause us to have to 
revalidate all our antibodies? My gut feeling is “no”, but if others out there 
have experience in which it did, I do hope they speak up.
 
We do not do any alcohol recycling. I have worked in labs where we recycled 
ethanol and it can work quite well provided you don’t try recycling 
xylene-contaminated alcohol. You’ll never get the xylene out of it; at best you 
can recycle it as xylene, but most of the reagent will just end up in the waste 
container. Best just to relegate it to waste in the first place.
 
As for why I am asking, the catalyst for this is our new fume hood. It goes 
into intermittent alarm mode when in contact with ethanol, but mostly small 
amounts of methanol. They recommend we use IPA. Because I know IPA has a long 
history of use in Histology processing, I think it could be a reasonable 
substitution without creating a lot of problems downstream. But, as always, I 
try to tread carefully before leaping.
 
Gudrun, I am planning on a simple exchange of reagent alcohol by isopropanol as 
usual. If we use it in our processor, I’d like to try to use it universally as 
much as possible. That’s why I asked about holding tissues in it, and using it 
in the stain line. The more exceptions we have in the lab (IPA on processor, 
SDA-3A on stainer, etc) the more potential for error when doing exchanges.
 
Thanks again,
 
Teri
 
From:Rene J Buesa [mailto:rjbu...@yahoo.com] 
Sent: Tuesday, August 13, 2013 8:41 AM
To: Teri Johnson; histonet@lists.utsouthwestern.edu
Subject: [Histonet] Isopropyl Alcohol in the histology lab
 
Teri:
It is a wise decision to substitute EthOL with Isopropyl alcohol (IPA) and to 
your questions:
1- although it is not advisable to keep tissues for long periods of time in 
EthOL sometimes this is done. EthOL will sometimes make the tissue brittle 
mostly because its chemical properties but IPA dehydrates more gently and is a 
larger molecule that EthOL so, if you use to keep tissues in 70EthOL you will 
be able to leave them in 70IPA more safely.
2- with a different dehydrating quality you will probably need to adjust your 
times in the auto-stainer or in your hand staining sequence. Your best option 
though is to totally eliminate xylene and the alcohols in your staining 
sequence. How? Dewax sections with 2% aq. dishwasher soap at 90ºC → distilled 
water → staining protocol → dry your stained sections in an oven for 5 minutes 
at 60ºC → coverslip as usual, but I am sure this may seem too drastic for you 
but I can assure you it works. Ask me if you want to.
3- Immnuno reactivity depends on how the tissue is FIXED and not in how it is 

[Histonet] Cryostat recommendations

[Rathborne, Toni]

Happy Friday everyone!
I'm looking for some feedback on cryostat preferences. We are replacing one of 
ours now and will be evaluating a few before making a final decision. The 2 
I've looked into so far are the Leica 1860 and the HM525 by Thermo Scientific. 
I'm interested in the UV feature that many now offer too and want to know what 
your experiences are. Does it require extra maintenance? Is it costly to 
repair/replace?  Do either of these models (or previous versions) have any 
known problems? They both look very nice on the outside, but are they made of 
durable materials internally? Thanks in advance for your responses, and have a 
great weekend!

Toni

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RE: [Histonet] (no subject)

[Rathborne, Toni]

Personal preference is something we try to accommodate within reason. Each of 
our  techs have their own tools (brush, forceps, etc.) which they take care of 
and clean after each use. The more comfortable a person is with a specific 
tool, the more accurate and efficient they will likely be. If your techs use a 
forceps that is not comfortable for them, think of tiny biopsies that could be 
compromised due to that. A hand can develop a cramp and tissue could be damaged 
by too much pressure, or could pop out of the cassette and become lost if it 
wasn't grasped correctly. I don't understand how standardizing the type of 
forceps used will create a Lean process.  I would look at the bigger picture 
and remember that histology is a skill performed by people, not instruments. 
Remember to evaluate the process. If techs need improved skills for embedding, 
that should be addressed with each one that is experiencing problems.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanjeet 
Dhirubhai
Sent: Wednesday, August 07, 2013 9:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)



 Hi,

I am trying lean up at the embedding system. We have issues where staff have 
their own preference in regards to working on a specific forceps. I am trying 
to standardize this process and eliminate the hassle of having different types 
of forceps. Can anyone help me. Thanks Regards,
 
Sanjeet Dhirubhai - Supervisor Histology  MLT 
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RE: [Histonet] Air bubbles in Coverslipper

[Rathborne, Toni]

We have had success with this also. If you got the unit new, I believe that 
there should have been one extra bottle with it.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Saturday, August 03, 2013 5:16 AM
To: 'Anna Huntley Coffey'
Cc: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] Air bubbles in Coverslipper

Hi,
We always have more than one medium-bottle prepared. At least three are filled 
in advance. So entrapped air can go away until usage and the oldest
is the one for exchange.

Gudrun

-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Anna Huntley 
Coffey
Gesendet: Freitag, 02. August 2013 21:56
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Air bubbles in Coverslipper

Hi Histonetters!

We have a Leica CV5030 coverslipper in our lab and we regularly (~ once a week 
or so) have trouble with large air bubbles showing up in a significant number 
of our slides.  We have flushed the hose from the mounting medium through the 
needle with xylenes which sometimes works to clear the bubbles and sometimes 
does not.  Has anyone else had trouble with this and possibly found a solution? 
 I would really appreciate your advice!

Thanks,
Anna
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[Histonet] Position in NJ

[Rathborne, Toni]

Community hospital in central NJ has FT, histotech position available, day 
shift.

Preferred qualifications include:
-ASCP  HT or HTL
-training with NAACLS approved laboratory on-line training program or ASCP 
recognized program for histology
-three or more years' experience in a histology laboratory
-ability to create, manipulate and utilize spreadsheets and word-processing 
programs

For a more complete description, or to apply on line, go to 
https://careers-somerset-healthcare.icims.com/jobs/1032/job or forward a resume 
directly to me at 
trathbo...@somerset-healthcare.commailto:trathbo...@somerset-healthcare.com .


Toni Rathborne
Pathology Supervisor
Somerset Medical Center






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[Histonet] RE: Leica CV5030

[Rathborne, Toni]

So would I. Once the initial quirks were worked out, it rarely has a problem. 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Thursday, March 21, 2013 3:26 PM
To: 'Bruce Gapinski'; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Leica CV5030

We love our Leica CV5030.  It rarely breaks a slide.  It works well with our 
Leica Bond labels.  I would buy another one.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas 
Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bruce Gapinski
Sent: Thursday, March 21, 2013 12:30 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Leica CV5030

This coverslipper is over engineered. No self respecting histologist would 
design something that holds the slide a foot in the air by two v shaped 
grippers. Gravity sucks and the slide falls. I have a box of DISTROYED slides 
that I show the repairman. Can you imagine Sorry Mr. Smith we can't read your 
biopsy because it splintered into pieces AFTER we did all the hard work. Good 
luck with that health of yours.
It is NOT compatible with Ventana labels, as the gum sticks to those grippers 
and the slide falls, or jams in the output rack.
To tell you the truth, I loved the old Leica coverslipper. It had a walking 
beam and never broke a slide.
I'm in the mood for a Sakaura.

Bruce Gapinsk HT (ASCP)
Chief Histologist
Marin Medical Laboratories
PathGroup SF




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[Histonet] RE: Tracking OR specimens

[Rathborne, Toni]

We are able to view a pending log. Once a patient is in the OR, nurses will 
place an order for the specimen. If we have not received the specimen in the 
anticipated time, we will call.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michelle 
Lamphere
Sent: Monday, March 18, 2013 9:14 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Tracking OR specimens

Are there any histology labs that actively participate in auditing the 
Operating Room on a daily basis to make sure that histology receives all of the 
specimens that the OR should have submitted?  If so, how do you do this?  Or 
should the OR be solely responsible for making sure that they specimens make it 
to histology?

Michelle M Lamphere, HT (ASCP)
Senior Tech, Histology
Children's Medical Center
1935 Medical District Drive
Dallas, TX  75235
Office :214-456-2798
Histology: 214-456-2318
Fax:  214-456-0779


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[Histonet] RE: Embedding centers

[Rathborne, Toni]

Maybe one of the used equipment vendors would consider a short-term lease with 
option to buy.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of MARCELLYN A. 
STONE
Sent: Wednesday, March 13, 2013 7:14 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Embedding centers

Good morning,
I have a tissue tek embedding center.  The hot plate no longer works.  
I have been told I can not get it replaced until July and it is too expensive 
to repair.  I have looked into barrowing or getting a loaner from someone who 
has a back-up.  That has not worked.  Anyone have any ideas on maybe a fix?   
Thanks Marcy
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[Histonet] RE: Placentas - Requests for Homeopathic remedy usage

[Rathborne, Toni]

This was discussed at great length last year. You can check the archives for a 
lot of responses.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim
Sent: Tuesday, March 12, 2013 2:49 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Placentas - Requests for Homeopathic remedy usage


We are occasionally getting requests for a patient to take home a placenta.   
Our hospital policy does not require that placentas are sent to pathology 
unless requested by the clinician. The placentas that are being requested are 
ones that normally would not come to pathology for examination and are 
supposedly being used in homeopathic placental remedies.

Currently our hospital policy does not allow for tissue to be given to the 
patient to take home.   I am interested in how other hospitals are handling 
these requests.

Thanks for your time.

Jim

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor Memorial Medical Center
217-788-4046



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[Histonet] RE: Waste Alcohol

[Rathborne, Toni]

We have been disposing of our alcohols like this for years. This would include 
alcoholic eosin too. We are allowed to mix the waste xylene with the alcohols 
because they are the same classification, and treated the same way.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
Sent: Monday, February 25, 2013 3:28 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Waste Alcohol

Hello to all in histoland.  Is there anyone putting alcohol into a waste drum 
like for xylene.  We have a company that takes away our xylene.  Now the safety 
people are suggesting that we treat the alcohol the same way.  We have always 
poured the alcohol down the drain.  It seems very expensive.  Your help in this 
matter will be greatly appreciated.



Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-5287
Lab: 713-566-5287


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[Histonet] RE: Process and hold?

[Rathborne, Toni]

We have actually had someone come in on Sundays just for this reason. An option 
for you might be to try this, and have that person put in a full day. He/she 
could then take off Monday. It has worked well for us. We also are able to get 
IHC cases done over the weekend so they can be given to the pathologist first 
thing Monday morning.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Sent: Tuesday, February 12, 2013 1:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Process and hold?

Hello all,

I was wondering about processing breast specimens (needle cores) on Fridays.

We have asked our radiology department to try to avoid scheduling these breast 
biopsies on Fridays since we do not work weekends and are concerned about the 
extended time in formalin.

I am thinking that we can run these specimens on a second processor over Friday 
night and have someone from the clinical lab come up  and drain the paraffin.  
The tissues would then sit in a warm moist retort until Monday morning when 
they would be embedded and cut.  I think the specimens would be fine.  
Processing would be complete at that point and they would hold in the unopened 
retort chamber.

Our alternative is to have someone come in every Saturday morning just to 
remove and embed these specimens.



Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org
www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org


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RE: [Histonet] Independent Equipment Service Companies

[Rathborne, Toni]

Where are you located?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stella Mireles
Sent: Tuesday, January 15, 2013 9:10 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Independent Equipment Service Companies

I lost the contact number I had for a company that will service histology 
equipment.
Could you please give me some contact info.

Thank You
Stella
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RE: [Histonet] automated microtomes

[Rathborne, Toni]

Thanks all for your valued opinions. I have contacted Leica, and am now waiting 
for a demo!

From: Jay Lundgren [mailto:jaylundg...@gmail.com]
Sent: Thursday, December 06, 2012 4:22 PM
To: Lynette Pavelich
Cc: Rene J Buesa; Rathborne, Toni; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] automated microtomes

Leica is making the sweetest 'tomes out there at the moment, IMHO.

 Sincerely,

   Jay A. Lundgren, M.S., HTL (ASCP)

On Wed, Dec 5, 2012 at 7:10 AM, Lynette Pavelich 
lpave...@hurleymc.commailto:lpave...@hurleymc.com wrote:
I am purchasing my second Leica RM2255 automated microtome. Rene' is correct in 
saying that the carpal tunnel syndrome will eventually affect every advanced 
tech by using any microtome manually. On the RM2255, the flywheel additionally 
is automated. You can choose to use the flywheel manually or automated by the 
simultaneous pushing of two buttons. Nice feature for us oldies to ease us 
into automation or if you have a tiny specimen that you need to take extra care 
with and want to use manually.
Companies are very happy to send in a demo to try for a couple weeks.

Happy shopping!! ;)
Lynette


From: 
histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu
 
[histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu]
 on behalf of Rene J Buesa [rjbu...@yahoo.commailto:rjbu...@yahoo.com]
Sent: Tuesday, December 04, 2012 1:19 PM
To: Rathborne, Toni; 
histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] automated microtomes

The advantage of the so called automated microtomes (the only thing automated 
about them is the block advance) is that they alleviate wrist effort and in 
some ways prevent carpal tunnel syndrome that affects some histotechs (mostly 
of the senior persuasion).
I would go with the Leica.
René J.

From: Rathborne, Toni 
trathbo...@somerset-healthcare.commailto:trathbo...@somerset-healthcare.com
To: 
histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu
Sent: Tuesday, December 4, 2012 11:48 AM
Subject: [Histonet] automated microtomes

I'm looking for some opinions about the automated microtomes currently 
available. Which ones do most techs prefer? Which are more reliable? Is there 
an advantage to having a semi-automated microtome?
Thanks in advance for your replies.

Toni


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[Histonet] automated microtomes

[Rathborne, Toni]

I'm looking for some opinions about the automated microtomes currently 
available. Which ones do most techs prefer? Which are more reliable? Is there 
an advantage to having a semi-automated microtome?
Thanks in advance for your replies.

Toni


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RE: [Histonet] Number of blocks

[Rathborne, Toni]

Although almost 20 years ago, the reference lab I worked in had similar 
expectations. They also had no idea that GARBAGE IN= GARBAGE OUT, and we had an 
unusually high number of recuts.  Smaller sections in the cassette or longer 
fixation would have reduced that number.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brendal Finlay
Sent: Thursday, October 25, 2012 10:21 AM
To: Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Number of blocks


Many years ago in histology training at AFIP, we were taught that the quota 
was 30 blocks an hour.  As someone stated before, certain tissue types are easy 
to cut and are 1-2 sections per slide making that 40-50 block/hr rate a bit 
reasonable. When you're leveling prostates, skins, cutting specials or 
unstained, working with dry, difficult, or fatty tissue, slide turn out time is 
increased. 


I remember recently seeing someone talking about cutting 80 blocks/hr and the 
folks I work with could see the multiple question marks above my head because 
that seems impossible to me at less than 30 seconds per block.  No offense to 
anyone who can do this.  More power to you!


I looked in a few histology books, but could not find a written reference on 
how fast a tech should cut.  Consistent, good sections placed on the slide in 
a neat manner should also be factored into the equation.


*hops off soapbox*


Brendal C.Finlay, HT (ASCP)

-Original message-
From: Dorothy Ragland-Glass techman...@yahoo.com
Date: Thu, 25 Oct 2012 08:10:20 -0500
To: Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov, Mike 
pencempe...@grhs.net, Histonet@lists.utsouthwestern.edu
Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Number of blocks

 No. My main duty is Ihc, but I heard the other techs, mostly the
ones new to histology and some older techs who informed them on how obsurd and 
impossible that task would be for them to try to live up to that standard. The 
newbees thought that was what the speed of a histotech should be. They were 
told it did not matter what the tissue was accordding to CAP. Us older techs 
know different. But we need written documentation to show the young turks who 
are being bullied.
Is there something to written to give them a leg to stand up.
 
 Bartlett, Jeanine (CDC/OID/NCEZID)wrote:
 
 Absolutely! 40-50 bone marrows is completely different from 40-50
fallopian tubes. Are you just cutting one section per block?
 
 Jeanine H. Bartlett
 Centers for Disease Control and Prevention Infectious Diseases 
 Pathology Branch
 404-639-3590
 jeanine.bartl...@cdc.hhs.gov
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence
 Sent: Thursday, October 25, 2012 8:50 AM
 To: Dorothy Ragland-Glass; Histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] Number of blocks
 
 As a histo lab supervisor I would never ask nor demand that my
techs do something that I cannot do myself. I would have to say that that 
number sounds a little high to me, but it woulddepend on the type of specimens 
being cut.
 
 Just my thought, Mike
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Dorothy Ragland-Glass
 Sent: Wednesday, October 24, 2012 7:38 AM
 To: Histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Number of blocks
 
 
 It was annouced by a histo lab manager that techs are expected to
cut
 40-50 blocks per hour. That seems to me to be rather high. I don't
see quality slides being turned out. It is quantity and profit above patient 
care. I am old school, and I remember something about quality and patient 
first. Besides what kind of impact on morality of the techs, back problems and 
carpal tunnel syndrom is laying ahead for the cutter after cranking the 
microtome repeatedly thatmany blocks without a break.
 
 
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RE: [Histonet] Pam Plumlee wants to share new pictures with you :)

[Rathborne, Toni]

I received and deleted.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison
Sent: Saturday, October 13, 2012 2:37 PM
To: Pam Plumlee; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Pam Plumlee wants to share new pictures with you :)

Has anyone else received this invite???  Not sure if it is legit? 

 
Akemi Allison BS, HT(ASCP)HTL
Director
Phoenix Lab Consulting
E-Mail: akemiat3...@yahoo.com





 From: Pam Plumlee paw...@yahoo.com
To: histonet@lists.utsouthwestern.edu 
Sent: Friday, October 12, 2012 5:50 PM
Subject: [Histonet] Pam Plumlee wants to share new pictures with you :)
 

Pam Plumlee has added you as a friend on Zoosk.

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RE: [Histonet] Problem in peloris processor

[Rathborne, Toni]

How long are your specimens fixed for before processing? 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
tahs...@brain.net.pk
Sent: Tuesday, October 09, 2012 10:17 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Problem in peloris processor

Dear All!

We processed the 3mm biopsies in peloris processor using factory 4 hrs.
xylene standard protocol.
But the processing was poor (soft) and almost half of the biopsies didn't 
process well reagent status was ok.
Advised require regarding this problem.
Regards.
Muhammad Tahseen
Pathology (Histology)
SKMCHRC



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[Histonet] RE: Breast processing times

[Rathborne, Toni]

We have someone come in on Sunday, and work with one less person on Monday.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig
Sent: Friday, October 05, 2012 11:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Breast processing times

Can you please give me your routine for processing breast cases over the 
weekend.  Do you extend the time in alcohol to achieve the 48 hour maximum, 
call someone in over the weekend, or just indicate on the patient report that 
the fixation extended beyond the 48 hours limit for HER 2, 72 hours for ER and 
PR.  Is the ischemic time recorded within the lab as well as on the patient 
report?

Thanks

Diana

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[Histonet] RE: thickness of slides

[Rathborne, Toni]

4 microns and 3 for lymph nodes.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig
Sent: Tuesday, September 18, 2012 2:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] thickness of slides

Can you tell me what thickness you cut your routine slides for HE and immuno 
(in particular lymph nodes).  

 

Also, your protocol for cutting prostate needle core biopsies.how many 
spares you cut and do you designate the level on the slide label if multiple 
levels are submitted on one slide?

 

Thanks

Diana

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RE: [Histonet] CAP autopsy requirement?

[Rathborne, Toni]

90% of the cases need to be signed out in 60 days, not all cases.

**NEW** 07/31/2012
ANP.33120 Final Report TAT Phase II
The final autopsy report is produced within 60 working days in 90% of the cases.
NOTE: The 90% threshold is used in recognition of the fact that occasional 
unusual cases may
require more than 60 days for completion, particularly when external 
consultation is required. If
cases exceed 60 days, there should be documentation of the reason for the delay 
and of ongoing
review of this information by the director of the service.
Evidence of Compliance:
✓ Review of turnaround time data for the final autopsy report
REFERENCES
1) Adickes ED, Sims KL. Enhancing autopsy performance and reporting. A system 
for a 5-day completion time. Arch Pathol Lab Med.
1996;120:249-253
2) Smith MT, Garvin AJ. Anatomic pathology turnaround times. Use and abuse. Am 
J Clin Pathol 1996;106(Suppl 1):S70-S73
3) Baker PB, et al. Quality assurance of autopsy face sheet reporting, final 
autopsy report turnaround time, and autopsy rates. A College
of American Pathologists Q-Probes study of 10 003 autopsies from 418 
institutions. Arch Pathol Lab Med. 1996;120:1003-1008
4) Hanzlick RL. The autopsy lexicon. Suggested headings for the autopsy report. 
Arch Pathol Lab Med. 2000;124:594-603
5) Bove KE, et al. The role of the autopsy in medical malpractice cases, II. 
Controversy related to autopsy performance and reporting.
Arch Pathol Lab Med. 2002;126:1032-1035
6) Hutchins GM, et al. Autopsy reporting. In: Collins KA, et al, eds. Autopsy 
Performance and Reporting. 2nd ed. Northfield, IL: College
of American Pathologists: 2003; chap 28
7) Baker PB. Communication of autopsy results. In: Collins KA, et al, eds. 
Autopsy Performance and Reporting. 2nd ed. Northfield, IL:
College of American Pathologists: 2003; chap 33
**

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias
Sent: Thursday, August 09, 2012 3:49 PM
To: 'mtitf...@aol.com'; 'histonet@lists.utsouthwestern.edu'
Subject: RE: [Histonet] CAP autopsy requirement?

If it's true, I hope our director doesn't share the information. We got the 60 
day thing down pat and all of our TAT reports. Hate to see it all go to waste.

Victor Tobias HT(ASCP)
Clinical Applications Analyst
Harborview Medical Center
Dept of Pathology Room NJB244
Seattle, WA 98104
vtob...@u.washington.edu
206-744-2735
206-744-8240 Fax
=
Privileged, confidential or patient identifiable information may be contained 
in this message. This information is meant only for the use of the intended 
recipients. If you are not the intended recipient, or if the message has been 
addressed to you in error, do not read, disclose, reproduce, distribute, 
disseminate or otherwise use this transmission. Instead, please notify the 
sender by reply e-mail, and then destroy all copies of the message and any 
attachments.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mtitf...@aol.com
Sent: Thursday, August 09, 2012 12:14 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CAP autopsy requirement?


Am I hearing right? The CAP has done away with the requirement to sign out 
autopsies within 60 days? (But you still have to get the PAD out in two days)? 
How will I ever get pathologists motivated any more?!

Michael Titford
Pathology
USA Mobile AL

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[Histonet] RE: DEEPER REQUESTS

[Rathborne, Toni]

For the biopsies that routinely have levels taken, our pathologists would 
prefer to order deepers rather than have a possibly diagnostic piece of the 
tissue trimmed away before obtaining a section. Yours may feel the same way.
With regard to the controls, our pathologists will specify which control they 
want to see at the time of the stain request, so we do not use both together.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L
Sent: Wednesday, June 27, 2012 10:45 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] DEEPER REQUESTS

Does anyone know if there is an average percentage that a histology lab should 
expect in deeper/recut requests?  I am trying to establish if our pathologists 
order more than the norm or if I have a quality issue unfolding!!

Also, do most labs use 2 controls for their pneumo staining, a routine fungus 
and a pneumo carnii positive?

Thanks ahead of time!
Dorothy Webb, HT (ASCP)
Regions Histology Technical Specialist



  
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RE: [Histonet] turnaround time

[Rathborne, Toni]

You also might do better calculating your end time not as signed out, but 
delivered to the  pathologist. By doing this, you can get a better 
understanding of where the bottlenecks occur. Your techs may get the slides out 
at 9:00, but the case may not be signed out for another 8 hours. 
We have PAs for the grossing.  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, June 21, 2012 12:07 PM
To: Nancy Schmitt; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] turnaround time

Your TAT will be determined by the way you organize your workflow. Under 
separate cover I am sending you data for several countries that I presented at 
a conference in Australia and another in Spain.
René J.



From: Nancy Schmitt nancy_schm...@pa-ucl.com
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Thursday, June 21, 2012 11:29 AM
Subject: [Histonet] turnaround time

Histonetters-

As we discuss new instrumentation and different workflow scenarios I am curious 
to know what kind of turnaround time others have from time of accession to 
report sign out.
1.  How long and what percentage?
2.  How many have HT's grossing in smalls (skins, gi's)?

Thank you much!
Nancy



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[Histonet] Urate crystals

[Rathborne, Toni]

We have a piece of bone that the pathologist would like to process for urate 
crystals. The procedure we have is De Galantha's, which calls for 
decalcification by nitric acid, which we don't have. What other decalcification 
process can be used?

Toni



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RE: [Histonet] Slide distribution amongst Pathologists

[Rathborne, Toni]

We do the same.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, 
Thomas
Sent: Tuesday, June 05, 2012 6:06 AM
To: susan.wal...@hcahealthcare.com; gu.l...@gmx.at; sa...@hotmail.ca
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide distribution amongst Pathologists

We have one simple way of doing it. He who Grosses is he who reads. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
susan.wal...@hcahealthcare.com
Sent: Tuesday, June 05, 2012 3:21 AM
To: gu.l...@gmx.at; sa...@hotmail.ca
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide distribution amongst Pathologists

Boy, they sure like to put us in the middle of what should be their own 
problem. Thank heavens I only work now for one Dr at a time now but when I was 
at a larger place they rotated. They still used to tell us to give them 
particular cases when it was not their turn so that we got the flak when 
someone did not get what they thought was theirs. You can never win! :)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Monday, June 04, 2012 2:34 PM
To: 'Sheila Adey'
Cc: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] Slide distribution amongst Pathologists

Here the slides go through the hands of one pathologist, who distributes the 
cases. 
Gudrun

-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Sheila Adey
Gesendet: Montag, 04. Juni 2012 20:19
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Slide distribution amongst Pathologists


Hello Netters:
I am looking for some ideas regarding slide distribution amongst pathologists.
Currently one Dr. reads all the surgicals and one other reads the cytology and 
bone marrows.
We now have 5 Dr.s and they are looking for ways to disperse the work evenly 
per day.
Thanks
:)
 
Sheila Adey
Charge Technologist
Laboratory - Histology Department
Bluewater Health
89 Norman Street
Sarnia, ON  N7T 6S3
519-464-4500 x 7063
 
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RE: [Histonet] Leica CV5030 coverslipper issues

[Rathborne, Toni]

Our CV5030 is 7 years old, and although it experiences problems occasionally, 
it is nothing that we felt it was necessary to have replaced. 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood, 
Margaret
Sent: Monday, May 21, 2012 1:57 PM
To: 'Linda'; histonet@lists.utsouthwestern.edu
Cc: kristy.han...@leica-microsystems.com; jack.ke...@leica-microsystems.com; 
paul.raimo...@leica-microsystems.com
Subject: RE: [Histonet] Leica CV5030 coverslipper issues

We have a refurbished CV5030 and Autostainer XL and have had no issues with 
them, or if so, minor ones that did not require contacting anyone. 

Peggy 


Peggy Sherwood
Research Specialist, Photopathology
Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherw...@partners.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Linda
Sent: Monday, May 21, 2012 1:58 PM
To: histonet@lists.utsouthwestern.edu
Cc: kristy.han...@leica-microsystems.com; jack.ke...@leica-microsystems.com; 
paul.raimo...@leica-microsystems.com
Subject: [Histonet] Leica CV5030 coverslipper issues

Hello Everyone,
 
 
I purchase a brand new Leica CV5030 coverslipper, which I received at the 
beginning of February.  I have had non- stop issues with this coverslipper from 
it throwing slides, coverslips and now the sensor not working properly.
 
I have emailed Leica several times now requesting a new coverslipper.  If I 
have this many issues at three months what is it going to do in a year?
 
The tech service support person has been great with fixing all of the issues.
 
This is my reply I received from the President, North America, Jack Kenny-
 
We do not believe that it is appropriate at this point to replace this system. 
  We will continute to monitor the situation but not upgrade at this time.
 
 
I am not looking for an upgrade I would just like a new coverslipper that works.
 
Has anyone else had problems with Leica not replacing defective equipment?  
Please let me know.  How did you resolve the issue?
 
Thank you in advance,
 
 
Linda Dee, BGS, HT(ASCP)
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RE: [Histonet] New Reagent Lot Verification

[Rathborne, Toni]

Thanks everyone!

-Original Message-
From: Settembre, Dana [mailto:sette...@umdnj.edu] 
Sent: Friday, April 13, 2012 11:03 AM
To: 'Sebree Linda A'; Rathborne, Toni; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] New Reagent Lot Verification

We are saving slides from inspection to inspection, about 2 years.
Dana Settembre
University Hospital - UMDNJ
Newark, NJ

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A
Sent: Thursday, April 12, 2012 11:26 AM
To: Rathborne, Toni; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] New Reagent Lot Verification

We're saving both for at least 2 years. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Thursday, April 12, 2012 9:50 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] New Reagent Lot Verification

ANP.22760 refers to new lot verification for antibodies and detection.
The Evidence of Compliance says that there should be Records of verification 
of new reagent lots. What is everyone's interpretation of records? Are you 
saving slides in addition to paper documentation?


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RE: [Histonet] Thank you

[Rathborne, Toni]

Not necessarily true. We used pencils for years and are now trying different 
markers because the pencil is smudging.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Louise Renton
Sent: Thursday, April 12, 2012 3:11 AM
To: Histonet
Subject: Re: [Histonet] Thank you

there's always good old pencil (HB) as a backup

On Thu, Apr 12, 2012 at 12:05 AM, Patsy Ruegg pru...@ihctech.net wrote:

 The best markers I have ever used are called KP Markers, they were off 
 the market for a while, but they are back and we get them from 
 Mercedes Medical, just got a new batch and they are wonderful just 
 like the old KP markers, we won't have anything else in the lab.

 Patsy Ruegg, HT(ASCP)QIHC
 IHCtech
 12635 Montview Blvd. Ste.215
 Aurora, CO 80045
 720-859-4060
 fax 720-859-4110
 www.ihctech.net
 www.ihcrg.org


 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim 
 Wheelock
 Sent: Wednesday, April 11, 2012 2:43 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Thank you

 Hi Everyone:

 I want to thank everyone who gave me advice concerning my cassette 
 labeling problem.
 I am trying out three different types of markers pens: StatLab, Leica, 
 and NewComer Supply Histo-tec brands.
 We will also make sure to let the ink dry before putting the cassettes 
 into formalin.
 If we still have a problem, we will experiment with a different cassette.
 Thanks again.

 Tim Wheelock
 Harvard Brain Bank
 McLean Hospital
 Belmont, MA
 617-855-3592

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--
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel  fax)
073 5574456 (emergencies only)
Question: Are rhinos  overweight unicorns?
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[Histonet] New Reagent Lot Verification

[Rathborne, Toni]

ANP.22760 refers to new lot verification for antibodies and detection. The 
Evidence of Compliance says that there should be Records of verification of 
new reagent lots. What is everyone's interpretation of records? Are you 
saving slides in addition to paper documentation?


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This message and any included attachments are from Somerset Medical Center
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promptly delete this message and notify the sender of the delivery error
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