Re: [Histonet] Antibody dilution help

part antibody in 100 parts total. Some folks read 1:100 as 1 part in 100, some folks read it as add 1 part to 99 to make 100 parts. confused yet?  I found this Let's see what others have to say. Sincerely, Paula Sicurello On Friday, August 30, 2024 at 07:57:36 AM PDT, Charles Rile

Re: [Histonet] Histo haiku

Pink and purple stainThe slide pink and not too blue.Benign, very good news Sincerely, Paula Sicurello On Sat, Jul 20, 2024 at 1:29 PM, Bob Richmond via Histonet wrote: Saffron, eosin, Stamens of autumn crocus: So yellow! So red! This actually is a haiku, with the obligatory seasonal

Re: [Histonet] De-mummification procedure?

Jay, Groaning and eye rolling. Sincerely, Paula Sicurello On Wednesday, May 15, 2024 at 12:06:24 PM PDT, Jay Lundgren via Histonet wrote: The Mummy's major weakness is fire, a common weakness among the undead. Since mummies tend to be dry and coated with various oils and r

Re: [Histonet] [EXT] Reagent alcohols and tissue processors

issue and ready for xylene and wax? Phillip Kara, HTL | Senior Research Associate University of North Texas Health Science Center Division of Research and Innovation  a: 3500 Camp Bowie Blvd, Fort Worth, TX 76107 p: 918-281-9060 w:www.unthsc.edu/corelabs From: Paula Sicurello via Histonet

[Histonet] Reagent alcohols and tissue processors

Good Morning, My question two part: Best tissue processor ever and why? What is percentage is your first alcohol step? I see that lots of places start at 70%  but I favor a lower %.  One because it's gentler on the tissue, and two because 10% NBF precipitates out if you start at 70%. Thanks for y

[Histonet] A Question for DOD Histology Labs

Good Morning and Happy Monday, If you work at a Department of Defense (DOD) Histology lab, can you tell me what make and model of cassette printer and slide writer you use? Do they have an ATO? We are in the market for both and want to know what y'all are using. Plus how do you like whatever it i

Re: [Histonet] tissue cassettes

Why use a different wax for embedding?  Cost. Those who don't fully understand the art & science of histology think a wax is a wax is a wax. Bon-bon, Paula Sent from Yahoo Mail on Android On Sat, Feb 10, 2024 at 11:23 AM, Carl Hobbs via Histonet wrote: Why would anyone use a different wa

[Histonet] Formalin pH

Hello Histoteckies, What are y'all doing regarding the CAP requirement to monitor the pH of formalin? We buy tons and tons of the 5 gallon cubitainers and we are still debating over how to check the pH. Looking forward to your replies. Toodles! Sincerely, Paula Sicu

Re: [Histonet] Logging everything you do.

s taking so long to get results.  This week I had one tell me she got her flow results in a day and Histo has had the specimens for two days - so what's the hold up? Please share what items are troubling after the lists are compiled. It will be interesting to see what they have to say.  Sinc

Re: [Histonet] Logging everything you do.

ri - ask them for to improve, streamline, etc.  Asking the staff for help to improve the lab goes a long way to making a good working environment.  With fewer and fewer people becoming histotechs - the work environment is crucial. Sincerely, Paula Sicurello On Saturday, July 22, 2023 at 01:0

[Histonet] QIHC

Well..I did not pass the exam on my second attempt.  Waa! Anyway, it got me wondering:  are there statistics out there that give a break down of the pass rate of the QIHC exam? What's the percentage of HTLs that are QIHC? Sincerely, Paula Sicu

Re: [Histonet] Sections sticking to tweezers

ompression that happens when being cut. Let's see what others have to say about your sticky situation. Paula Sicurello On Sat, May 6, 2023 at 8:27 AM, Ken M via Histonet wrote:Can anyone tell me why my sections are sticking to my tweezers and turning into one long string of snot when I try to s

Re: [Histonet] cutting with glassknifes

27;t do that anymore.  I have to use a knifebreaker. Paula Sicurello On Sun, Apr 30, 2023 at 9:40 AM, Ross Langston via Histonet wrote: Hello- I do not work in a conventional histology lab, but we exclusively use resin (JB-4) Embedding and glass knives on MT-1s to do histology on gonadal

Re: [Histonet] Tissue Processor Schedule Validations

small biopsies.   Has anyone changed it?  Or do folks go along with it just because it's already there?  I inherited that temp, to change it now would be pain since it is the one we use the most.  If I validated our current Peloris 3, I would have set the wax temperatures at 60 degrees. Sincer

Re: [Histonet] Mice/Rat brain

cool? Wrinkles.   Can you use vibratome sections or do you need FFPE sections? Paula Sicurello On Thu, Mar 9, 2023 at 6:51 AM, Renee Fisher via Histonet wrote: We are having issues with mice and rat brain sections. There are folds in the tissue. We just can’t seem to get a flat section

Re: [Histonet] Who sells that block deparaffinizer dohickey that everyone has in their lab.

Ted Pella, Inc.  (Support small business)  they sell EM, and Histology items. Paula Sicurello On Thu, Mar 2, 2023 at 5:19 AM, Karen Heckford CA-San Francisco via Histonet wrote: I got mine through Thermo Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center

[Histonet] Cassette printers

Data, Sakura, and Leica. Please provide your opinions and related experiences with each of the vendor's cassette printer.  We currently have a General Data CL12 that is dying a slow, painful death. Thanks oodles for your help. Paula Sicurello __

Re: [Histonet] Epon Resin Embedding Question

) has had luck dealing with blocks like those. Sincerely, Paula Sicurello On Wed, Sep 28, 2022 at 12:27 PM, Cooper, Brian via Histonet wrote: Good afternoon Histonet, Asking this question for a friend:  Is there a way to correct an improperly prepared Epon resin embedded block?  Foll

[Histonet] QIHC certification

gestions anybody? Thanks oodles. Sincerely, Paula Sicurello ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Part Time Histologist Needed In San Diego, California

Hello Listers, UC San Diego Health has an opening for a part-time Histologist.  HT/HTL required. Experience in a high volume academic setting preferred.  We need someone with strong sectioning and trouble shooting skills.  Familiarity with IHC or Electron Microscopy a plus. Go to jobs.ucsd.edu a

[Histonet] UC San Diego Anatomic Pathology Histology Labs is Hiring

Hello, We are hiring a histotechnologist for our growing histology lab.  UCSD is adding a new hospital and soon our volume will increase.  We are looking for an experienced histotech.  Please go to:  https://jobs.ucsd.edu/bulletin/job.aspx?cat=health&sortby=post&jobnum_in=81586.  Sincerely, Paula

Re: [Histonet] Start up Vet/Animal histology laboratory

Hi Linda, I've set up three histology labs for animal research: one in an academic setting, one for a pharmaceutical firm, and one for a research foundation.   1. Having money to buy the proper equipment is tricky, start with used equipment and make due until you can afford to buy new.  As long a

[Histonet] VIP 6 owners manual

Hello Netters, Does anyone have an electronic copy of the owners manual for a Tissue Tek VIP6?  If you do can you send it to me? Sakura charges $129 for one!! Thanks, Paula UC San Diego Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] 10% NBF Question

couple of weeks. I know that Richard-Allan recalled their NBF a couple of years ago because the % was 0-3 not 10%. Please let me know if any of you are experiencing something similar. Sincerely, Paula Paula Sicurello Histotechnology Specialist UC San Diego Health

[Histonet] Results of My Histology Survey-Finally!

Well Listers, I finally get around to posting my survey.  Only 35 people took it but it still provided useful information and I got an A in my class. Thank You to the 35 who participated All the data:             23 HT's, 8 HTL's, 4 with other certification (2 with HT/HTL) took my survey        

[Histonet] Traveling Histologists?

Good Morning Listers, Are there companies out there that work with traveling histologists?  If so, can you send me their contact information? Companies dealing with traveling histologists are free to contact me as well.  Sincerely, Paula Paula SicurelloHistotechnology SpecialistUC San Diego Health

[Histonet] Workload and Productivity Survey closing this week

Good Morning Listers, I will be closing my survey this week on Friday.  If anyone else would like to take it, please follow this link: Histology Questionaire Survey |   | |   |   |   |   |   | | Histology Questionaire SurveyWeb survey powered by SurveyMonkey.com. Create your own online survey no

[Histonet] Histology Questions part 2

Good Evening Netters, I've created a short survey using Survey Monkey.  Please take my survey and help me gather information I can use in my class and to help update the productivity standards and staffing benchmarks for our Histology labs. https://www.surveymonkey.com/r/8HFSY3V Thank you and th

[Histonet] Histology Lab Survey Questions

Hello Netters, Thank you for taking my survey.  I will use your replies for my research project and I will also let everyone on the list know the results as well. From what I've found, the surveys performed in the past asked similar questions but more at the institution level.  I would like to ge

[Histonet] Histology Related Questions?

Hello Netters, I am taking a research techniques class for my MBA this term and need to have a project that I can ask quantifiable questions.  I was thinking about productivity and benchmarks in the Histology and IHC labs. If I send an email with my questions, will you be kind and answer them in

[Histonet] Histology Related Questions?

Hello Netters, I am taking a research techniques class for my MBA this term and need to have a project that I can ask quantifiable questions.  I was thinking about productivity and benchmarks in the Histology and IHC labs. If I send an email with my questions, will you be kind and answer them in

[Histonet] Test email

Good Morning Netters, This is a test to see if I can get an email through using this email account. My posts haven't been making it through and I know you miss me. Sincerely, Paula Paula Sicurello Histotechnology Specialist UC San Diego H

[Histonet] Source for ultra low temperature immersion thermometer

below -100 degrees C. Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UCSD Medical Center 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the

[Histonet] CAP Question

ems a bit much. Enquiring minds want to know. Thanks a bunch! Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UCSD Medical Center 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended onl

[Histonet] H&E Stainer Question

Me again... UCSD is in the market for a new H&E stainer for our new hospital opening next year. We need a workhorse, not a prima dona, something with a coverslipper built in would be nice. What do you use? Suggestions gratefully accepted-even from you two Keith and Matt ;) Opinions about the

[Histonet] Per Diem Positions at UC San Diego Health System

Good Afternoon Listers, UCSD Medical Center Department of Anatomic Pathology has two per diem positions open in Histology. Histology experience is a must. If you are interested email your resumes to: Craig Fisher at *cfis...@ucsd.edu * and Mike Mathhew at *mimatt...@ucsd.edu * Thank you and

[Histonet] A question about a new CAP requirement

Hello Netters, How are you all interpreting this new requirement (COM.4) with regards to multiple autostainers for IHC? Thank you in advance, Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mai

[Histonet] COM.40000 (new)

Hello Netters, How are you all interpreting this new requirement with regards to multiple autostainers for IHC? Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] BS in Histotechnology

fingers off - I wouldn't even try to teach them to use a microtome. > > > -Original Message- > From: Michael Ann Jones > To: Stedman, Nancy ; Mark Turner > ; Paula Sicurello > Cc: histonet ; Timothy Morken < > timothy.mor...@ucsf.edu>; Jennifer MacDonald ; &g

Re: [Histonet] BS in Histotechnology

nt to > try to read. I know I can't. > > > > -Nancy Stedman > > > > > > > > > > -Original Message- > > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Turner &g

[Histonet] Another question: C4d and preeclamptic placentas

Hello Netters, plus Matt and Keith ;-) I'm picking the collective brain of the Histonet... I've found some literature that suggests that up to 50% of preeclamptic placentas that resulted in a negative outcome have diffuse C4d staining when using IHC. Has anyone tried C4d IHC or IF on preeclampt

[Histonet] C3d?

Hello Again My Dear Netters, One of our pathologists was wondering if anyone out in Histoland is performing a C3d stain? If so what type: IHC, IF? Thanks oodles! Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsou

[Histonet] Positive Control for IF?

Good Afternoon Netters, Since we went down the path of hot dogs and Slim Jim's as positive controls for FFPE stains, I was wondering. Is there a source, like a hot dog or piece of steak, that could be a positive control for immunofluorescence C4d? What I'd really like to find is some food or

Re: [Histonet] BS in Histotechnology

I've had more than one pathologist tell me a monkey could do my job. Though one of them said it with a smile and added "a very highly skilled and well trained monkey", he was one of the few who knew better. How many of us monkeys have trained the whining and complaining residents how to do things

[Histonet] GMS control

Thank you to all who volunteered ideas and control blocks. Of course, the control must be of human origin, so they are digging through their files to find a nice fungusy patient tissue for me. Darn those CAP regulations, they take all the fun out of it. I still want to try an orange or Slim Jim

[Histonet] GMS controls?

Good Afternoon Netters, I have a project to validate a special stainer and will be starting with GMS. What can I use as a good source of fungus? Was it last week there was mention of orange peels and onions? I need to get the OK of the attending pathologist, so I probably can't use anything too

Re: [Histonet] Embedding Question

help minimize the risk of losing blocks during > transport. > > Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Beh

Re: [Histonet] Embedding Question

om > > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas > Sent: Thursday, March 12, 2015 10:32 AM > To: Paula Sicurello; HistoNet > Subject: RE: [Hi

Re: [Histonet] Embedding Question

l Studies > Department of Pathology > UC San Francisco Medical Center > > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello > Sent: Thursday, March 12, 2015 6:29

[Histonet] Embedding Question

It has been proposed to move the embedding centers to a room about 210 ft away from the tissue processors. The trip from processor to embedding center would take over 2 minutes and require the histotechs to carry the baskets full of cassettes down a much used hallway. Opinions? Do you feel this

[Histonet] How dark is dark enough?

Good Morning Netters, While running immunofluorescence stains, how dark is dark enough? I have worked in labs where we did them in full room light, almost complete darkness (developing negative/film darkness), and somewhere in between. I feel that dimmed lights are good enough. What does the hi

Re: [Histonet] And other crazy stuff.

I forgot to mention: dirt Happy Friday Paula On Fri, Jan 9, 2015 at 2:39 PM, Shirley A. Powell wrote: > Orangutan testicle macro section and alligator jawbones, not my best work, > very humbling, after 52 years in the business. > Shirley > > > -Original Message- > From: histonet-boun

Re: And other crazy stuff. RE: [Histonet] cutting honey bees

You asked for it: squid tentacle tip, frog oocyte, serial sections through a entire zebra fish embryo, honey bee eyes, harbor seal artery, Andean mummy muscle (flaky little boogers), probably lots of others that I can't remember off the top of my head. Paula On Thu, Jan 8, 2015 at 3:20 PM, Micha

Re: [Histonet] On the lighter side...

> Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.uts

[Histonet] Clinical EM Labs?

clinical EM as well. I figured, if we are a small group, we should get to know each other. Thanks and have a nice weekend. Sincerely, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North

[Histonet] IHC imaging systems

buying a new one. It needs to have an algorithm that allows the system to distinguish between labelled and not labelled cells and the percentage of each. Thanks in advance, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm

[Histonet] Second Question of the Day: -80 degrees rationale

as long as possible. The lab that had this freezer previously had an acceptable range of -70 to -90. Thanks in advance, again, oh wise ones. Paula -- > Paula Sicurello, HTL (ASCP) > Supervisor, Clinical Electron Microscopy Laboratory > Duke University Health System > Rm.#251M, Duke Sout

[Histonet] -80 degrees rationale

responses! Thanks in advance, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying

[Histonet] -80 freezer question

-- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic

Re: [Histonet] Remove OCT from frozen tissue block

_**_ > Histonet mailing list > Histonet@lists.utsouthwestern.**edu > http://lists.utsouthwestern.**edu/mailman/listinfo/histonet<http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Micros

Re: [Histonet] Microtome Blade safety, in or out when not in use?

Left in but covered with the blade guard. Not spanking new, but usable (for facing) get stored in an old box that the slides came in. I like the slide mailer idea, and will switch to that. -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health

[Histonet] External UV for a Leica 1850?

infectious tissue is cut in it. Suggestions kindly welcomed. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification

Re: [Histonet] More about the good old days..

I had a co-worker who would drink CocaCola mixed with the sealed, glass flask 100% ETOH every Friday. -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091

Re: [Histonet] Re: bunsen burner at the embedding center

im to work, both ways in a rip tide-battleing sharks and jelly fish, while sloshing around in an earthquake! It is San Diego after all. Happy Friday! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green

Re: [Histonet] RE: Bunsen Burner

No open flames allowed. Bill, If you use nitrile gloves, the sticky buns don't stick! -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Pr

Re: [Histonet] EPIC Beaker

tentative go-live for July 2014. Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are

Re: [Histonet] microtome aligner

raight at 90 degrees. You take the knife holder out and place one side of the L on the knife holder place and the other against the block face. Paula :-) -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Gree

Re: [Histonet] Gross Room cleaner

Dispatch works well too. Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Tue, May 14, 2013 at 12:28 PM, Amber McKenzie < amber.mc

Re: [Histonet] Isopentane storage-the polling is open

Hi Julia, Thanks for the information. Can you tell me why you store it in the refrigerator? Paula Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Tue

[Histonet] Isopentane storage-the polling is open

refrigerator, my experiences are with storing it at room temperature. What do you all do? Room temp or refrigerated? Any and all comments are greatly appreciated. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M

Re: [Histonet] Possible CJD and decontaminating Leica ASP300s

Lori, You need to contact the National Prion Disease Center at http://www.cjdsurveillance.com/ They will tell you if there is a way to decontaminate anything that may have come in contact with the suspected CJD case. Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron

Re: [Histonet] RE: Temperatures

We have NIST traceable digital thermometers that can have the min/max reset. We have been told that if you reset on Friday and check on Monday morning the min/max & it is in range-put an OK by the date. -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory

Re: [Histonet] 4% Paraformaldehyde

Rhonda, What Tim says is true, it all depends on what the tissue is going to be used for. Provide more detailed information to get a more detailed answer. Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South

Re: [Histonet] No expiration dates on chemicals

We keep ours no longer than 5 years. If there is no expiration date on the bottle when we receive it, we mark it as expired 5 years from date of receipt. Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South

Re: [Histonet] ATPase without barbital buffer... info

Hi Tim, Thanks for finding those protocols for us! Perhaps we can all move away from using a controlled substance (what a nightmare to purchase). Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green

Re: [Histonet] RE: Permanent Fluorescent Mounting Media

James, When I was at MCV we used Crystal Mount for our frozen IF's (skin, kidney, etc.) You put a few drops on, place it in the oven for a little bit (I do not recall the temperture of the oven) and you had a nice hard cover over your sections. I hope this helps. Paula -- Paula Sicurello

Re: [Histonet] 88305TC starting to hit the fan...

Sure they want more physicians. My husband was forced to leave medical school when he became sick (they wouldn't give him a medical leave of absence). No other medical school would touch him after that. They thought something was fishy, his transcript reads "medical leave of absence" which wasn'

Re: [Histonet] Formalin and Operating Rooms

I would be interested in the replies as well. To add to that, I've heard the same thing about glutartaldehyde, do the same rules apply? Thanks, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green

[Histonet] Beaker module in EPIC

se, send me any of the pluses or minuses that you've experienced using Beaker. Thanks, Paula :-) -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27

[Histonet] Epic Beaker module

me know if your experiences and what if anything have you had Epic build into it for you. Thanks, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091

Re: [Histonet] Diamond Knives

for additional resharpening. There are other brands on the market, I have not used them in a while so I cannot comment about those vendors. If you have anyother questions, feel free to ask. Paula :-) -- Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health

Re: [Histonet] broken chuck holder

You should observe this tech to see what he/she is doing to break 3 in 2 years. Then you can have a training session in the proper use of the chuck. Either that or let them know that eating spinach before cutting is not a good idea On Thu, Nov 17, 2011 at 6:42 AM, Essex, David wrote: > On

Re: [Histonet] Retirement

Sheesh! We used to have people smoke while working with propylene oxide. Eating in your control pigs was part of the benefit of being a graduate student to save on grocery money. Film? My TEM used glass plates. Lab mates used to routinely drink diet coke and 100% ethanol on Fridays. Wearing c

Re: [Histonet] OT: How do you call...

This reminds me of a joke I learned as a kid. How to use analyze and anatomy in a sentence (song actually). "My analyze over the ocean, my analyze over the sea, my analyze over the ocean, so bring back my anatomy." :-) -- Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope

Re: [Histonet] colloidion

Is there any reason why the pathologist can't used agar? It's very simple to use and non-toxic. -- Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On F

Re: [Histonet] Section position on slides

lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P:  919.684.2091 __

Re: [Histonet] Microtome alignment

ces > > 611 West Park Street > > Urbana, Illinois 61801 > > 217-383-3572 > > sharon.davis-dev...@carle.com > > > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.ed

Re: Re-thinking Nigerian Scam ! [Histonet] FW: NEEDS URGENT HELP !!!

s in the safe at the hotel. We need to get back home, but we >> have no way to get to our flight in Germany that leaves for home next >> week. >> >> Right now our only option is to fly home from here. We need some money so >> we >> can purchase tickets to get to JFK, and then fly

Re: [Histonet] Breezy lab/flyaway ribbons!

rohibited. If you are not the intended recipient, please > contact the sender by electronic mail and destroy all copies of the original > message. > ___ > Histonet mailing list > Histonet@lists.utsouthweste

[Histonet] Best books for the HTL?

of folks who went through the grinder recently. Thanks, -- Paula Sicurello 6 of 6 Duke University EM Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] BSL-2 question

Thanks to all who can give me any pertinent information. Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for you

Re: [Histonet] RE: OT: Early Friday Hour of Fuming

ion in staffing when everyone knows that I'm the only one, talk about lack of job security. Grrr, hey at least the sun is shining and there are blue skies! Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jol

Re: [Histonet] EM processing

the tissue is 1mm cubed or less) and 2 days in the oven for the resin to polymerize. You can speed things up if you microwave process. If you have any questions, please feel free to contact me. Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro

Re: [Histonet] Organizing of cassettes for processing

the missing cassettes by the embedding stations. Plus they could embed the block in numeric order so the slides that needed to get out first were cut and stained first. Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La

Re: [Histonet] water on slides?

I'm getting the same eosin bleeding and water on the slides. I'm using Hemo-D and CitriSolv. I have changed the reagents and am still getting the eosin bleeding, the water on the slide is a new phenomenon. Any suggestions? Paula Sicurello VA Medical Center San Diego Vetera

RE: [Histonet] Cleaning oil off objectives

my 5 objectives are no longer objective after having their lenses clouded by the media. ;-) Paula Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for

[Histonet] Support for core units

ly 80% time and the only person down here. Should I start looking for a new position before she shuts me down? How many people have had their facilities closed by the people who don't see the big picture? Please let me know your experiences. Thanks, Paula :-} Paula Sicurello VA Medi

[Histonet] Core unit support

ly 80% time and the only person down here.  Should I start looking for a new position before she shuts me down?  How many people have had their facilities closed by the people who don't see the big picture? Please let me know your experiences.  Thanks, Paula  :-} Paula Sicurello VA Medi

Re: [Histonet] Clear-Rite 3

.  I work in the research unit of a VA hospital and they will not provide larger fume hoods nor buy me a self contained processing sytem. Please let me know what other safe alternatives (ones that can be used out on the bench) are you suggest. Thanks, Paula Sicurello VA Medical Center San Diego

RE: [Histonet] Start Up Lab

You might still have your fingers, but are your fingerprints still the same?  Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center (CRIC) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 Your images

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