[Histonet] Frozen sections: H and TMA questions

Hi, I'm at a new lab where we use Leica Infinity stains for our frozen H stains. I've noticed our stains are very pale and poorly differentiated. Does anyone have experience with this particular Leica product? Or do you guys have a recommendation for a better H stain kit? At my previous job we

Re: [Histonet] Frozen sections and cold acetone

I have always "fixed" in RT acetone for 10 mins Have compared 0-60 mins/4C to RT acetone Lower temps just limit the rate of reaction, imho. I note "nuclear streaming" when I use acetone at any temp/time. Imho, acetone is not an effective fixativeit's a delipidiser. So, give it 10 mins at RT.

Re: [Histonet] Frozen sections and cold acetone...

Cold acetone, and cold ethanol, were used to fix tissues because they left enzymes unaffected and still demonstrable. This was in the early days of enzyme histochemistry. Pearse' Histochemistry: Theoretical and applied,3rd edition, volume 1, page 85 discusses it. I could send a scan if you

[Histonet] Frozen sections and cold acetone...

Can anyone give me a rational for using cold (refrig or freezer-temp) acetone to fix frozen sections? Or a rational for using RT acetone. This is for kidney or muscle bx frozens for immmunofluroescence or immunoperoxidase staining. Normally they air dry for at least 15 minutes (just waiting

Re: [Histonet] frozen sections

Lab Services UW Health - UW Hospital 600 Highland Avenue Madison, WI 53792-2472 -Original Message- From: Eck, Allison via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, May 30, 2018 7:25 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] frozen sections

[Histonet] frozen sections

Question for those of you who do not have PA's...Who cuts the frozen sections, the techs or the pathologists? Thanks in advance Allison Allison Eck, HTL(ASCP)cm,QLS, AHI(AMT),CEAS1 Lead Tech Histology Doylestown Hospital 595 W State St Doylestown, PA 18901 215-345-2264

Re: [Histonet] frozen sections

rn.edu] Sent: Tuesday, June 20, 2017 5:30 PM To: histonet@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] frozen sections Tissue that does not go through formalin processing does not need AR. Sent from Yahoo Mail on Android   On Tue, Jun 20, 2017 at 4:52 PM, Isabe

Re: [Histonet] frozen sections

: [Histonet] frozen sections Tissue that does not go through formalin processing does not need AR. Sent from Yahoo Mail on Android On Tue, Jun 20, 2017 at 4:52 PM, Isabel Soto via Histonet<histonet@lists.utsouthwestern.edu> wrote: Yes. Sent from Yahoo Mail on Android On Tue, Jun 20, 2017 at 3

Re: [Histonet] frozen sections

Tissue that does not go through formalin processing does not need AR. Sent from Yahoo Mail on Android On Tue, Jun 20, 2017 at 4:52 PM, Isabel Soto via Histonet wrote: Yes.  Sent from Yahoo Mail on Android   On Tue, Jun 20, 2017 at 3:15 PM, Nair, Indu

Re: [Histonet] frozen sections

Yes.  Sent from Yahoo Mail on Android On Tue, Jun 20, 2017 at 3:15 PM, Nair, Indu via Histonet wrote: Hello Histoneters! question: Does frozen section need Antigen Retrieval? thank you, for the anticipated timely help! indu

[Histonet] frozen sections

Hello Histoneters! question: Does frozen section need Antigen Retrieval? thank you, for the anticipated timely help! indu - -SECURITY/CONFIDENTIALITY WARNING- This message (and any attachments) are intended solely for the

[Histonet] Frozen Sections

I have a question that I wanted some input on.We recently did a frozen section and the patient was confirmed hepatitis B.I was wondering if anyone had any protocols for the stains and the cleanliness of the slides after the frozen section was retrieved. This is this the first time this

RE: [Histonet] Frozen Sections

- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Monday, February 09, 2015 7:03 AM To: 'wanda.sm...@hcahealthcare.com'; lsmal...@juno.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Frozen Sections

RE: [Histonet] Frozen Sections

'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Frozen Sections We provide assistance while the dept. is staff with histology techs. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of lsmal...@juno.com

RE: [Histonet] Frozen Sections

; 'lsmal...@juno.com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Frozen Sections we do, during the day Monday - Friday (8:00-17:30) but not over the weekend -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] Frozen Sections

@lists.utsouthwestern.edu Subject: [Histonet] Frozen Sections Hi, I need to ask Histoland a questionHow many HT departments provide assistance to the pathologist in the performance of frozen sections (cutting and staining of slides) to be evaluated by the pathologist? Thank you very much in advance

RE: [Histonet] Frozen Sections

same Joelle Weaver MAOM, HTL (ASCP) QIHC From: mpe...@grhs.net To: lsmal...@juno.com; histonet@lists.utsouthwestern.edu Date: Mon, 9 Feb 2015 14:04:57 + Subject: RE: [Histonet] Frozen Sections CC: We provide assistance while the dept. is staff with histology techs

RE: [Histonet] Frozen Sections

@lists.utsouthwestern.edu Subject: [Histonet] Frozen Sections Hi, I need to ask Histoland a questionHow many HT departments provide assistance to the pathologist in the performance of frozen sections (cutting and staining of slides) to be evaluated by the pathologist? Thank you very much

Re: [Histonet] Frozen Sections

We provide assistance, primarily on the outpatient frozens, but help with in-patient ones as well. What makes you ask? Michelle Sent from my iPhone On Feb 6, 2015, at 10:37 PM, lsmal...@juno.com lsmal...@juno.com wrote: Hi, I need to ask Histoland a questionHow many HT departments

[Histonet] Frozen Sections

Hi, I need to ask Histoland a questionHow many HT departments provide assistance to the pathologist in the performance of frozen sections (cutting and staining of slides) to be evaluated by the pathologist? Thank you very much in advance! Lorraine

[Histonet] Frozen sections for Immunoflouresence

Lately we are experiencing an issue where the tissue sections are coming off of the slide during the staining process. We have always used positive charged slides and have not experienced this problem on a routine case. We do not fix the slides but allow them to air dry for Immunology.

[Histonet] frozen sections

Hi All, Thanks for all of your help with the slippery floors. I was also wondering which personnel labs are using for performing frozen sections? Lab Assistants? Histotechs? What are your minimal requirements? Do you classify frozen sections as high complexity testing? Thank you, Lacie

[Histonet] Frozen sections

Good morning, I know all histotechs help cut and stain frozen sections. Is there any organization out there that has the histotechnician gross the frozen section tissue and place on the frozen section chuck to cut without the Pathologist in the room. If anyone does this please tell me why?

Re: [Histonet] Frozen Sections Falling Off Slides

Hello netters...have someone tried doin frozen in a tissue mistakenly immersed in formalin??not so long but the inside still fresh..m sure the slides will wash out..fresh always better.. On Jun 17, 2012, at 10:34 PM, Emily Sours talulahg...@gmail.com wrote: Wow you can run frozen sections

RE: [Histonet] Frozen Sections Falling Off Slides

:34 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Frozen Sections Falling Off Slides Wow you can run frozen sections through those machines? That's awesome. I figured it wouldn't be gentle enough to process them. I'm a fan of Fisher Scientific superfrost plus slides. Fisher

Re: [Histonet] Frozen Sections Falling Off Slides

Wow you can run frozen sections through those machines? That's awesome. I figured it wouldn't be gentle enough to process them. I'm a fan of Fisher Scientific superfrost plus slides. Fisher claims the slides are electrostatically charged to keep the sections from falling off. Whether that's a

[Histonet] Frozen Sections Falling Off Slides

Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen section Machine...But i dont understand why i got problems sections falling off slides.I used different types of slides non adhesive,adhesive(poly l lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections taken

Re: [Histonet] Frozen Sections Falling Off Slides

PM Subject: [Histonet] Frozen Sections Falling Off Slides Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen section Machine...But i dont understand why i got problems sections falling off slides.I used different types of slides non adhesive,adhesive(poly l lysine,thermoshandon

Re: [Histonet] Frozen Sections Falling Off Slides

Was your leica set that cold? Maybe you could try warming the slide for a couple seconds before putting in alcohol. As far a charged slides I am amazed with the slides Dako sells for their IHC. Sent from my iPhone On Jun 16, 2012, at 12:01 PM, Bernadette del Rosario badzros...@yahoo.com

[Histonet] Frozen sections

Question: When doing frozen section on a lung mass (not fatty- just regular), and it chatters, what is the cause? The cryostat was set at -26, we used the normal OCT media, and cut at 5microns. I prefer not so cold (like -23 to -24) and I cut sections at 4, 5 , and even 6 to see if it

RE: [Histonet] Frozen sections

] Frozen sections From: Maria Katleba maria.katl...@stjoe.org Date: Tue, October 19, 2010 10:27 am To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Question: When doing frozen section on a lung mass (not fatty- just regular), and it chatters, what is the cause? The cryostat

[Histonet] Frozen sections missing centers

Hello, I have been asked to to prepare frozen sections of fish livers that have been stored in RNAlater Solution at -80C. Tissues (about 10 um square x 5 um thick) were embedded in OCT, frozen on dry ice, and sectioned at 6 um. My problem is that when I cut a section, the center of the tissue

: [Histonet] frozen sections of cartilage

Topics: 1. Fire in the lab (Cheryl) 2. Fw: [Histonet] frozen sections of cartilage (Kim Merriam) 3. Pathologist Assistant Jobs (Alisha Dynan) 4. RELIA Histology Careers Bulletin 03/01/10 Are you into Facebook? MySpace? Twitter? or Linkedin? (Pam Barker) 5. CD31 in rats (Annette

Fw: [Histonet] frozen sections of cartilage

 Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA - Forwarded Message From: Kim Merriam kmerriam2...@yahoo.com To: Histonet histonet@lists.utsouthwestern.edu Sent: Mon, February 22, 2010 2:37:58 PM Subject: [Histonet] frozen sections of cartilage Hi Everyone, Any tips for keeping frozen

[Histonet] frozen sections of cartilage

Hi Everyone, Any tips for keeping frozen sections of cartilage from falling off the slides during IHC staining?  We should not have to do HIER, but they still fall of the slides quite easily. Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___

Re: [Histonet] frozen sections of cartilage

...@yahoo.com wrote: From: Kim Merriam kmerriam2...@yahoo.com Subject: [Histonet] frozen sections of cartilage To: Histonet histonet@lists.utsouthwestern.edu Date: Monday, February 22, 2010, 2:37 PM Hi Everyone, Any tips for keeping frozen sections of cartilage from falling off the slides during IHC

RE: [Histonet] Frozen Sections Slides per Day Question

Hi all, Thank you so much! for all of your suggestions.    Rene, How many slides per case, if FS standard is 15 min/case?   Thanks again.   Soofia --- On Sat, 2/6/10, Rene J Buesa rjbu...@yahoo.com wrote: From: Rene J Buesa rjbu...@yahoo.com Subject: RE: [Histonet] Frozen Sections Slides per

RE: [Histonet] Frozen Sections Slides per Day Question

The norm is 2 (max. 3) slides per case. Your figure of 25 FS in 8 hours = 19.2 min/FS but you do a lot more that just 2-3 slides/case. René J --- On Mon, 2/8/10, soofia siddiqui soofi...@yahoo.com wrote: From: soofia siddiqui soofi...@yahoo.com Subject: RE: [Histonet] Frozen Sections Slides

Re: [Histonet] Frozen Sections Slides per Day Question

Dear Soofia Sissiqui, 25 blocks (12 slides for each block with 3-4 sections)as per Welcan Units code # 5019 entire day ( 8 hours 480 minte) Muhammad Tahseen Histology Supervisor SKMTHRC Lahore Pakistan Hi dear histology experts, I will greatly appreciate if somebody can let me know the

RE: [Histonet] Frozen Sections Slides per Day Question

] Frozen Sections Slides per Day Question Dear Soofia Sissiqui, 25 blocks (12 slides for each block with 3-4 sections)as per Welcan Units code # 5019 entire day ( 8 hours 480 minte) Muhammad Tahseen Histology Supervisor SKMTHRC Lahore Pakistan Hi dear histology experts, I will greatly appreciate

[Histonet] Frozen Sections Slides per Day Question

Hi dear histology experts, I will greatly appreciate if somebody can let me know the estimated number of slides (with 3 sections per slide) per day on average a lab technician can cut (of frozen tissue).   I am a lab technician ( not a histotech) and work alone in a highly complex testing

[Histonet] frozen sections not adhering to slide

I am processing adult rat brain sections for routine histological stains, but am having a terrible time getting them to stick to the slide. After transcardial perfusion the rat brains are dissected out and processed for frozen sectioning. I post-fix overnight in PFA, cryoprotect in

[Histonet] frozen sections not adhering to slide

I am processing adult rat brain sections for routine histological stains, but am having a terrible time getting them to stick to the slide. After transcardial perfusion the rat brains are dissected out and processed for frozen sectioning. I post-fix overnight in PFA, cryoprotect in

[Histonet] frozen sections for in situ hybridization

Hi, I have obtained fresh rat brains (not fixed) that have been directly snap-freezed in isopentane and stored at -80C. I plan to section 20um thick. I want to know if there is way to store the cut sections before I carry out the in situ hybridization without thawing and freezing? taht is, the cut