the above mentioned. I have tried and look it up and it seems
all over the place on what to do.
Any help would be greatly appreciated.
Hello Karen,
Long time no see! I always used Charged slides, and prefixed Methanol for
~ 5 min @ RT (not 95% alcohol) before staining (SS & IHC).
Hope this h
slides in the fridge, but "apple green birefringence" will also
fade with time (unstable).
*Madeleine Huey, **B.S., HTL & QIHC (ASCP)*
Supervisor, Pathology/Histology & IPOX (MV & LG)
El Camino Hospital
2500 Grant Road (Rm GC-33)
Mountain View, CA 94040
Phone: 650-940
Hello,
Does anyone have Covid 19 FFPE tissues or Transient/Stable Cell Line
(slides/block) ? I have some H.Pylori blocks for exchanges.
*Madeleine Huey, B.S., HTL & QIHC (ASCP)*
Supervisor, Pathology/Histology & IPOX (MV & LG)
El Camino Hospital
2500 Grant Road (Rm GC-33)
Mount
Subject: [Histonet] P16 Antibody
Jason, I also tried many different vendors & clones many years ago (human
FFPE tissues), and the winner is from Roche/Ventana (CINtec, Cat. 9517 =
11.5ml). You can get 5ml dispenser as well.
*Madeleine Huey**, HTL & QIHC (ASCP)*
Supervisor - P
Dawn,
Our TAT is 94% with 24 hours 98% within 48 hours (~ 6% complicated cases
need more IPOX/Molecular Study/FACS etc.).
Madeleine
Superviser - Pathology (IPOX Histology)
madelein...@elcaminohospital.org
From: histonet-boun...@lists.utsouthwestern.edu [mailto:
From:Huggins, Haley - MRMC haley.hugg...@dignityhealth.org
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Wednesday, September 11, 2013 4:46 PM
Subject: [Histonet] Equipment selection
Hi all,
I am trying to decide on what equipment to purchase. I have picked the
Message: 2
Date: Fri, 25 Jan 2013 19:00:57 +
From: Celebre Julia celeb...@hhsc.ca
Subject: [Histonet] SDH methods for muscles
To: 'histonet@lists.utsouthwestern.edu'
histonet@lists.utsouthwestern.edu
Message-ID:
722701352b76e841bfb9efaf72553cd2a09...@ipexmailm02.hhsc.ca
Hello,
Anyone know the Vendor for TMA recipient blocks (3mm)? I saw a
distributor carry them in the last CSH (Walnut Creek, CA).
madelein...@elcaminohospital.org
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Betsy,
Try the Paraplast Plus X-TRA. You can mix X-TRA with Plus (3:1) ratio
is too hard for your blade.
Good Luck!
madelein...@elcaminohospital.org
From: bmolin...@texasheart.org
To: Histonet@lists.utsouthwestern.edu
Date: Fri, 11 Jan 2013 17:31:02 +
CC:
Subject: [Histonet] harder paraffin
freezing in OCT
- Embed with OCT on ICE, not liquid nitrogen
- Pre-warm -20c before cutting
Good Luck!
Madeleine Huey, BS, HTL QIHC (ASCP)
Supervisor - Pathology (IPOX Histology)
madelein...@elcaminohospital.org
Message: 1
Date: Thu, 10 Jan 2013 15:16:32 -0600
From: Colleen Forster cfors
Accu-edge microtome blades from Fisher Scientific.
madelein...@elcaminohospital.org
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Wednesday, January 09, 2013 9:56 AM
To:
Hello Gale,
I normally ask the candidates questions relating to
embedding/cutting/special stains.
For example Special Stains;
- how do they perform a test for collagen and smooth muscle (answer -
Special Stain Trichrome). If they answer correctly with Trichrome,
then ask them WHY Trichrome
Hi Tasha,
Warthin-Starry will work very well. We used Dako Autostainer -
Artisan in our lab, but manual will work as well.
Good Luck!
Supervisor - Pathology (IPOX Histology)
Madeleine Huey BS, MT (CSMT), HTL QIHC (ASCP)
madelein...@elcaminohospital.org
Message: 4
Date: Thu, 3 Jan 2013 11:22
Chirayu,
I am not surprise you have this problem, it's a classic issue for many
lab (if they don't do research).
Any time if Mouse embryo are older than Day 13 (E13), you need a small
sharp scissor to slit open the mouse neck/skull abdominal ( E13) in
order to have properly fixation. It got
Kimura,
The answer to your question is YES. Both Tween 20 Triton 100 are
non-ionic detergents. Tween 20 is not as strong/effective as Triton
100, but you can use higher percentage Tw20 or prolong the
permeabilization timing.
Best!
Madeleine Huey, BS, MT, HTL QIHC (ASCP)
madelein
be resolved if you have a good rapid tissue
processor (ie. Leica - Peloris, etc). We have the same problem with
VIP5 before I purchased the Peloris. I haven't have one single
re-process since then.
There is a trick to my problem. Give me an email if you want to know.
Madeleine Huey, HTL QIHC (ASCP
hunting!
Madeleine Huey BS, HTL QIHC (ASCP)
Supervisor - Pathology (Histology IPOX)
madeleine_h@elcaminohospital
Hi Everyone:
I am currently evaluating three tissue processors.
They are the Sakura VIP6, the Leica ASP 6025, and the Thermo-Fisher
Excelsior ES.
I was wondering if people could
). The thinner the
block (horizontal) the lesser sections I need to cut.
Hope this answer your questions.
Madeleine Huey BS, HTL QIHC (ASCP)
Supervisor - Pathology (IPOX Histology)
madelein...@elcaminohospital.org
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. Therefore if you put them in the beginning
(ie.70%, 85% ) and not the 100% alcohol, they will eventually be
wash out.
Madeleine Huey BS, HTL QIHC (ASCP)
Supervisor - Pathology (IPOX Histology)
madelein...@elcaminohospital.org
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for addition 5min - 10min @ -20c
4th) Air dry slides with fan for ~ 5 min (or RT for 10 min). I
purchase $10 portable fan from drug store
5th) Wash off any excess OCT with washing buffer 3x
6th) Proceed your IHC staining..
Good Luck!
Madeleine Huey BS, HTL (ASCP) QIHC
Supervisor - Pathology
madelein
of small biopsies in my lab yet.
Good Luck!
Madeleine Huey BS, HTL (ASCP) QIHC
Supervisor - Pathology (IPOX Histology)
madelein...@elcaminohospital.org
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chamber (seal container with parafilm).
If none of these titration work, then purchase a better antibody for
your application.
Good Luck!
Madeleine Huey BS, HTL (ASCP) QIHC
Supervisor - Pathology (IPOX Histology)
madelein...@elcaminohospital.org
tissues
(Cat. # ab28364);
http://www.abcam.com/CD31-antibody-ab28364.html
Hope this help!
Madeleine Huey BS, HTL (ASCP) QIHC
Supervisor - El Camino Hospital (Pathology)
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http
All,
ER, clone SP1 is availuable in www.epitomics.com (cat. #4200-1) for $205.
Hope this help!
Madeleine Huey
Supervisor - Pathology (IPOX Histology)
madelein...@elcaminohospital.org
On Fri, Jul 13, 2012 at 7:08 AM,
histonet-requ...@lists.utsouthwestern.edu wrote:
Send Histonet mailing list
Hello Histonetters,
We are looking for someone who have a lot of experience in writing SOP
policies for Histology IHC (ie.CAP check-list). You can do them at
home (if your retiree) or in our lab. We will pay your service.
If you know someone who might be interested please tell them to
contact
, (2) Biocare Diva AR
buffer, (3) Pascal pressure cooker.
Keep us posted what's your outcome.
Madeleine Huey BS, HTL/QIHC (ASCP)
Supervisor - Pathology (histology IPOX)
madelein...@elcaminohospital.org
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Histonet
we can help you if you give us a more detail protocol.
Best,
Madeleine Huey BS, HTL (ASCP) QIHC
Supervisor - Pathology (IPOX Histology)
madeleineh...@elcaminohospital.org
Date: Wed, 18 Apr 2012 10:04:35 -0400
From: Clare Thornton cthorn...@dahlchase.com
Subject: [Histonet] PAX-8 antibody
green counterstain.
Madeleine Huey BS, HTL/QIHC (ASCP)
Supervisor - Pathology (IPOX Histology)
madeleineh...@elcaminohospital.org
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will be better; Not recommend for ER/PR, HER2)
4) Load to Leica Bond with ER2 for 20 min (or your usual protocol)
This should work because I have done it many times in different labs
(you might lost some tissues, but most of them should stay on the
slide (manufacture dependant).
Good Luck!
Madeleine
email me for more detail if needed.
Madeleine Huey BS, HTL (ASCP) QIHC
Supervisor - Pathology (IPOX Histology)
madelein...@elcaminohospital.org
On Sat, Feb 25, 2012 at 10:01 AM,
histonet-requ...@lists.utsouthwestern.edu wrote:
Send Histonet mailing list submissions to
histonet
slide (ie. vWR Fisher) they work
just fine.
Madeleine Huey BS, HTL (ASCP) QIHC
Supervisor - Pathology (IPOX Histology)
madelein...@elcaminohospital.org
On Wed, Feb 22, 2012 at 10:01 AM,
histonet-requ...@lists.utsouthwestern.edu wrote:
Send Histonet mailing list submissions to
histonet
to the slide with
excellent morphology.
Good Luck!
Madeleine Huey BS, HTL/QIHC
Supervisor - Pathology (IPOX Histology)
madelein...@elcaminohospital.org
On Wed, Feb 15, 2012 at 10:01 AM,
histonet-requ...@lists.utsouthwestern.edu wrote:
Send Histonet mailing list submissions to
histonet
cut
Good Luck!
Madeleine Huey BS, HTL (ASCP) QIHC
Supervisor - Pathology (IPOX Histology)
madelein...@elcaminohospital.org
On Fri, Jan 27, 2012 at 8:10 AM,
histonet-requ...@lists.utsouthwestern.edu wrote:
Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu
.24050
Mostly greatly any helps!
Madeleine Huey BS, HTL (ASCP) QIHC
Supervisor - Pathology (IPOX Histology)
madelein...@elcaminohospital.org
On Sat, Jan 21, 2012 at 10:00 AM,
histonet-requ...@lists.utsouthwestern.edu wrote:
Send Histonet mailing list submissions to
histonet
) Wrap lens paper put in cassette for process
If you need more more information, you can email me.
Madeleine Huey BS, HTL (ASCP) QIHC
Supervisor - Pathology (IPOX Histology)
El Camino Hospital
madelein...@elcaminohospital.org
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Refine detection kit
2) HIER; ER2 for 20 min
My IHC staining are always strong and consistent (manually by hand,
Dako Plus, or Leica Bond).
Hope this help!
Madeleine Huey BS, HTL (ASCP) QIHC
Supervisor - Pathology (IPOX Histology)
El Camino Hospital
madelein...@elcaminohospital.org
which
condition work best for your antibody.
Good Luck!
Madeleine Huey BS, HTL (ASCP) QIHC
Supervisor - Pathology (IPOX Histology)
El Camino Hospital
madelein...@elcaminohospital.org
On Wed, Jan 11, 2012 at 7:51 AM,
histonet-requ...@lists.utsouthwestern.edu wrote:
Send Histonet mailing list
Madeleine Huey BS, HTL (ASCP) QIHC
Supervisor - Pathology (IPOX Histology)
madelein...@elcaminohospital.org
On Sat, Jan 7, 2012 at 10:00 AM,
histonet-requ...@lists.utsouthwestern.edu wrote:
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histonet@lists.utsouthwestern.edu
To subscribe
and they work very well. They are very specified
and high affinity (appx. ~ 1:10k) with Dako's Envision + system.
Your problem will be solve if you just switch your primary ab from
mouse to rabbit.
Good Luck!
Madeleine Huey BS, HTL (ASCP) QIHC
Supervisor - Pathology (IPOX Histology)
madeleineh
.
Please let me know if my suggestion are helping or not.
Good Luck!
Madeleine Huey BS, HTL/QIHC (ASCP)
Supervisor-Pathology (IPOX Histology)
El Camino Hospitial
madelein...@elcaminohospital.org
On Thu, Dec 29, 2011 at 10:00 AM,
histonet-requ...@lists.utsouthwestern.edu wrote:
Send Histonet mailing
Andria,
I purchased my HSV I/II from Cell Marque (RTU). They work very well
with Dako Plus Leica Bonds.
These antibodies does NOT required antigen retrieval.
Dako Plus:
1) Deparafinize hydration
2) Quench with Dako Dual Endogenous Block (5')
3) Incubate with 1st ab (HSV I or HSV II) (60')
4)
Miha,
You need to lower down the cryostat at least -25 degree. You also need
to spray your block the very minute before the cutting (not just one
time spray on the OCT block). The thicker the section, the easier it
can be cut.
I also put my OCT blocks on the dry ice next to me, and transfer to
...@elcaminohospital.org
On Thu, Dec 22, 2011 at 10:54 AM, Madeleine Huey
madeleineh...@gmail.com wrote:
Miha,
You need to lower down the cryostat at least -25 degree. You also need
to spray your block the very minute before the cutting (not just one
time spray on the OCT block). The thicker
...@lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific
than Re: Contents of Histonet digest...
Today's Topics:
1. Re: Histonet Digest, Vol 97, Issue 20 (Madeleine Huey)
2. RE: CD31 for rat tissue (Patsy Ruegg)
3. e-cadherin (Patsy Ruegg)
4. RE
buffers used by IHC)
4) Wash with water
5) Dehydrate cover slip with permanent mounting
You can write or call me if you still need help or have any questions.
Madeleine Huey BS, HTL (ASCP) QIHC
Supervisor-Pathology
El Camino Hospital
Mountain View, CA
madelein...@elcaminohospital.org
On Sat, Dec 17
I have an immediately Per Diem Histotech position available in
Mountain View, CA. The hours are somewhat flexible, with 4 hr - 5/day
(Monday to Friday). Candidate must be able to embed approx. 50
blocks/hr and cut 100 - 200 blocks/day.
Madeleine
Supervisor-Pathology
El Camino Hospital
2500
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