Re: [Histonet] PAS Stain

Only a crazy Aussie would do this!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Depar

[Histonet] PAS Stain

developed a 10 minute room temp amylase method: Mangan, V-M, Farago, V., Kelly, M., Henwood, A.F., (2002) "An Amylase Reagent with a Long Shelf Life for the removal of Glycogen from Tissue Sections" J. Histotechnol 25:153. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC),

histonet@lists.utsouthwestern.edu

this variant of the H&E. Anyone have any ideas? Eosin Y (CI 45380) 5g Erythrosin B (CI 45430) 5g Sodium Hydrogen Carbonate 1.25g Magnesium Sulphate 10g Distilled water 500ml Regards Tony Henwood

Re: [Histonet] Porcessing FFPE tissue without alcohol??

ique for the demonstration of extracellular lipid in paraffin-embedded tissue sections. Journal of Histotechnology, 7(2), 75-77. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct

Re: [Histonet] H&E staining

I would look at the dewaxing stage. Have you changed waxes? Try longer in xylene &/or longer in the oven. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, Schoo

Re: [Histonet] Fungus contamination on AFB slides

Diagnostic Cytopathology 41(5):453-455 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 P

Re: [Histonet] Dako Artisan special stainer

Oh dear, Are you considering sacking your Special Stains Tech? Only kidding. I wish I had the money to buy one myself. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjun

Re: [Histonet] Tissue processing validation

The validation would already have been done. You only need to check if the re-location has affected any equipment components. I would take a few cassettes of spare tissue and process it on your most used program and await the results. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys

Re: [Histonet] Suggestions for staining ground substances in the Heart

rarely stain at pH 1)? 2. Is the alcian blue dye of good quality. There are several out there - use Certified dyes. Hopefully this helps Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at

Re: [Histonet] IHC repeat rates

The RCPA (in Australia) recently requested that laboratories perform Technical Failure Repeat Audits that might be of use. Subsequently I produced a brief report for our Society's journal: Audit of Technical Failures Tony Henwood Histopathology The Children's Hospital at Westmead,

Re: [Histonet] plants in the lab

Laboratory Air-conditioning systems should be separate from patient area systems (as is required for operating theatre units). I understand this to be good practice. From: Goins, Tresa [tgo...@mt.gov] Sent: Thursday, 4 June 2015 12:18 AM To: Tony Henwood

Re: [Histonet] [EXTERNAL] Re: plants in the lab

s sit on awaiting transport? We cannot live in a bubble. Paula Pierce, BS, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Norman, OK 73069 405-759-3953 PH 405-759-7513 FAX www.excaliburpathology.com From: "Goins, Tresa" To: Tony Henwood (SCHN) ; Mi

Re: [Histonet] tissue fixation-formaldehyde concentrations which is best.

John, I totally agree Tony From: John Kiernan [jkier...@uwo.ca] Sent: Saturday, 6 June 2015 2:31 PM To: Peter Noyce; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] tissue fixation-formaldehyde concentrations which is best. Dear Pet

Re: [Histonet] plants in the lab

Hi Michelle, Why would patients be in a histo lab anyway? From: Michelle Lamphere [michelle.lamph...@childrens.com] Sent: Sunday, 31 May 2015 10:36 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] plants in the lab Our hospital Safety and

Re: [Histonet] PAS stain

Hi Mica, Most haematoxylins will do eg Harris or Mayer's. I would recommend a light counterstain (it is a counterstain, not the main stain). Differentiate if too strong. Also periodic acid treatment increases nuclear affinity for Hx so shorten the counterstain time. Regards, Tony _

Re: [Histonet] Toluidine blue

Hi Bernice, Your researcher probably has no idea. I can demonstrate cartilage, mast cells, bacteria and mucins depending on the tol blue technique I use. Ie not all tol blue techniques are the same. Ask him what he is after. Without control I would not recommend his work for publication if asked

Re: [Histonet] GMS Question

Hi Teresa, Very interesting. Do you have any references on mature and immature fungal cell walls and how they react histochemically? Regards Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children’s Hospital at Westmead Adjunct Fe

Re: [Histonet] long answer RE: GMS Question

ungi are PAS positive but GMS negative. Pseudo-fungi will give a positive reaction with a GMS stain using periodic acid instead of chromic acid Regards Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children’s Hospital at Westmead

Re: [Histonet] GMS Question

I would strongly suggest that you not use Periodic acid for the GMS, its alright for the PAS but for the GMS. Use chromic acid as described the standard texts. Regards Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children’s Hosp

Re: [Histonet] FW: IHC and oven temperature

others were un-affected. Also, my caveat on this, is the importance that formalin cross-linking has in protecting some antigens from the denaturation of high temperature drying, which may not have been investigated as yet. Regards Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA

Re: [Histonet] IHC and oven temperature

omplex and variable. I will send you a copy of the paper in a separate email Regards Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel:

Re: [Histonet] IHC and oven temperature

: Rene J Buesa [rjbu...@yahoo.com] Sent: Friday, 1 May 2015 5:56 AM To: Tony Henwood (SCHN); Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC and oven temperature What about HIER at 95-98ºC? "Everybody" uses it to "enhance" epitopes detection. Is there not an int

FW: [Histonet] IHC and oven temperature

pril 2015 5:51 AM To: Tony Henwood (SCHN); WILLIAM DESALVO; Preiszner, Johanna Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC and oven temperature I remember reading that the preffered temperature was about 60 degrees Celsius. I think that this was in the Dako education gui

RE: [Histonet] IHC and oven temperature

Hi temp drying shown to be a bad idea: Henwood, A., (2005) “Effect of Slide Drying at 80°C on Immunohistochemistry” J Histotechnol 28(1):45-46. Abstract Prolonged high temperature dry heating has been found to be deleterious to the immunohistochemical demonstration of several antigens in forma

[Histonet] RE: Nuclear "Artifact"

This seems like a classic case of drying of biopsies prior to fixation. This can occur if biopsies are placed on absorbent paper (or on disinfecting alcohol swabs, heaven forbid). From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.uts

RE: [Histonet] Verhoeff's hematoxylin

Suggested Answers below 1. several hours is all you will get out of the solution I am surprised there is a method that gives you a month shelf life unless it is a different method eg orcein 2. If it works keep using it 3. Allowing the alcoholic Hx to ripen for at least a few days will make

[Histonet] RE: cleaning glassware

Also check the gelatine - is it a new batch? From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Anne Murvosh [amurv...@advancederm.net] Sent: Friday, 3 April 2015 6:01 AM To: Jeff Halstead; histonet@lis

RE: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek

Also check the last alcohol and xylene. Has someone accidentally replaced these solution with formalin by mistake? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children’s Hospital at Westmead Adjunct Fellow, Schoo

[Histonet] RE: Kidney bx transport

Try a cell culture solution like Hanks. It preserves better than normal saline (which despite its name is not isotonic with cells). It will keep a renal and skin biopsy quite well for at least an hour. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory

RE: [Histonet] Intestinal mucus -- Carnoy or what?

Since the mucus will tend to be water soluble. I would suggest 10% formaldehyde in alcohol. Fix for the usual time, dependant on size. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmea

RE: [Histonet] Jores, Klotz and that pesky chloral hydrate

totechnol. 29: 173–176. Oliveira, F. S. (2014). Assessing the effectiveness of 30% sodium chloride aqueous solution for the preservation of fixed anatomical specimens: a 5‐year follow‐up study. J. Anat. (2014) 225, pp118-121 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC),

[Histonet] RE: Processed blocks before decaling

I agree Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Depar

[Histonet] RE: Squamous cells staining on H&E and IHC

Would you believe that according to a few very imminent histotechnologists, the source of nearly all of the squames contaminating slides is dandruff (a little embarrassing!!) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scien

[Histonet] RE: Decalcified Bone Eosin Overstaining

* decrease time in eosin (or use a neutral 1% eosin stain). This should increase nuclear staining and decrease the eosin reaction. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjun

RE: [Histonet] mounting media for immunofluorescence

temperature. (Espada, J., Juarranz, Á., Galaz, S., Cañete, M., Villanueva, Á., Pacheco, M., & Stockert, J. C. (2005). Non-aqueous permanent mounting for immunofluorescence microscopy. Histochemistry and cell biology, 123(3), 329-334) This might be worth a try Regards Tony Henwood JP,

[Histonet] RE: Frozen sections for Immunoflouresence

Hi Jim, You mention that the OCT seems a little softer. Could it be that the sections are thicker than you usually produce. Check the Frozen section H&E to check this. If sections are thicker they will tend to detach from the slides. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys

RE: [Histonet] Is there a Law for refusal of pathology services.

I agree. Whatever the reason unfortunately, or fortunately, the patient has the right to refuse treatment. Though in this case seems very silly. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Ho

RE: [Histonet] Gayle Callis, You Made Our Monday Morning!!

Where is my "like" button??? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9

RE: [Histonet] Peggy Wenk's passing

This is so very sad. A great lady who will be missed by the international histotechnology community. A knowledgeable and patient educator. I hope we can live up to her memory and mirror her professionalism in our own careers. Let's make her proud of us. Regards Tony Henwood JP, MSc, B

[Histonet] RE: GMS better than PAS for Fungi

GMS is not to demonstrate pseudo-fungi. These are usually PAS positive. We would rather not have patients treated with anti-fungals if they do not actually have a fungus infection. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scien

[Histonet] RE: Churukian Silver stain for Fungus

s recommendation in his later article: Churukian, C. J. (1996). Consistent silver methods for demonstrating basement membranes, reticulum, and fungi. Journal of histotechnology, 19(3), 211-217. I follow Charles recommendations for paraffin sections (ie chromic acid). Regards Tony Henw

[Histonet] RE: Churukian Silver stain for pneumocystis and fungus

s recommendation in his later article: Churukian, C. J. (1996). Consistent silver methods for demonstrating basement membranes, reticulum, and fungi. Journal of histotechnology, 19(3), 211-217. I follow Charles recommendations for paraffin sections (ie chromic acid). Regards Tony Henw

[Histonet] RE: Churukian Silver stain for Fungus

echnology, 36(2), 45-50. Song, D. E., Kahn, A. G., Khang, S. K., & Ro, J. Y. (2005). Pseudofungi in pericolic lymph nodes. Archives of pathology & laboratory medicine, 129(4), e97-e100. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Mana

[Histonet] GMS better than PAS for Fungi

rongly PAS (and hence PASM) positive obscuring the small microorganisms). Using PAS, Old fungi, Mucor, Actinomyces and Nocordia do not stain well whereas they stain quite well with GMS. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Se

[Histonet] RE: Acid Clean Glassware

PAS, Old fungi, Mucor, Actinomyces and Nocordia do not stain well whereas they stain quite well with GMS. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of

RE: [Histonet] Problems with processors and dehydration

y won't be reached - though from what you have stated this is not the case) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Weste

RE: [Histonet] Mercedes Medical

No Adds on Histonet!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Patho

RE: [Histonet] Dried out liver bx's

Hi Sarah, 90% of the time it is a collection issue. Do they leave it on filter (or any other absorbent) paper before placing in fixative. OR, heaven forbid, are they putting it on one of those alcohol wipes they use for disinfecting skin before biopsy? Regards Tony Henwood JP, MSc, BAppSc

RE: [Histonet] Microwave in histological/histochemical protocols

Yep We use microwave for rapid processing of renal, cardiac and liver biopsies and Accelerated formalin fixation of tissues and Copper stain using rhodanine, Luxol fast blue for myelin Sulphated Alcian blue for amyloid Orcein for elastic fibres Regards Tony Henwood JP, MSc, BAppSc

RE: [Histonet] How to De-stain trichrome

verse (anyone trying to restain PAP smears might know the problem). This may cause problems. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, Univers

[Histonet] RE: artifact caused by freezing of tissues during transport

2004). Incidental freezing artefacts in sentinel lymph node biopsies masquerading as lymphangiography artefacts. Journal of clinical pathology, 57(6), 671. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospita

RE: [Histonet] Silver in Nucleolus

e Specificity of the Silver Staining Technique for Ag NOR-ASSOCIATED Acidic Proteins in Paraffin Sections. Biotechnic & Histochemistry, 59(3), 181-185. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children&#

RE: [Histonet] myeloperoxidase on FFPE or frozen sections

Yep, We use a rtu from Leica on the Bond3 platform (PA0491) with EDTA (high pH) antigen retrieval Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of

[Histonet] RE: TAT for Transplant Biopsies

utine renal biopsies as well. There seems to be less leaching out of cytoplasmic substances compared to the routinely processed biopsies. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead

[Histonet] RE: Freezer for tissue storage

Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the c

[Histonet] RE: On-slide IHC control workflow?

igens the control slides are used for. There might be issues with aged pre-cut slides but we have not noticed ant deterioration with the antibodies we use. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children'

[Histonet] RE: Rush bx schedule?

Hi Tim, Try fixing for 45 min at 50oC. I think it possible that the tissue is not adequately fixed. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of

RE: [Histonet] RE: Yahoo link NO PHI

Oh Poop I mean Damm Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318

[Histonet] RE: Chris van der Loos

I agree Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Depar

RE: [Histonet] Chris van der Loos

This is very sad. Chris was knowledgeable, with a wonderful sense of humour and always had time for his colleagues. He will be missed Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmea

RE: [Histonet] Frozen IF Specimen Storage: Is -80 C really necessary; will -20 C work?

keeps freezer walls and shelves free of ice, although ice-cubes will continually sublimate. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, Unive

[Histonet] RE: Peggy Wenks award

Congratulations, well deserved from down-under Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street,

RE: [Histonet] Cautery-Like Artefact

In this case I would also look at fixation time - increase it as much as possible. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkes

RE: [Histonet] Cautery-Like Artefact

skin, can result in morphology not unlike that seen after cautery. I have also seen it in biopsies that had been inadvertently placed on "alcowipes" (pads soaked in alcohol used for disinfecting skin prior to procedures) as well as biopsies placed on absorbent filter-like cards. Rega

RE: [Histonet] edge artefact on core needle biopsy

Hi Gudrun, I hope you are well. Another cause of anomalous staining is drying of the biopsy prior to fixative immersion. This can result in non-specific immunohistochemical staining as well as sub-optimal histochemical staining. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC

RE: [Histonet] (no subject)

And ditto again Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead

[Histonet] RE: Waterbaths

Hi LeAnn, We empty the waterbaths each afternoon and add fresh warm water in the morning (we do not need to use distilled water - fortunately Sydney has good clean water, as well as high taxes, high house prices, etc etc!!) We clean with warm soapy water. Regards Tony Henwood JP, MSc, BAppSc

[Histonet] RE: immunohistochemistry help please

n to techniques for the demonstration of fungi: a comparison to routine xylene-alcohol dewaxing" J Histotechnol 36(2):45-50. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the chi

RE: [Histonet] Tissue

Hi Mike, Try the butchers (liver, kidney, tripe, heart, brain etc) - very cheap Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury

RE: [Histonet] Study Highlights Risk of Errors in Processing Biopsy Tissues in Anatomic Pathology Labs

Joelle, Could you possibly be referring to: Platt E, Sommer P; McDonald L, Bennett A, Hunt J (2009) "Tissue Floaters and Contaminants in the Histology Laboratory" Arch Pathol Lab Med 133:973-978 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laborato

[Histonet] RE: Carnoys solution

y need to use the original Carnoy's recipe, though Meier's Fixative (Glacial acetic acid 25ml + 95% ethanol 475ml), with less acetic acid has been used in the past. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist

[Histonet] RE: non-xylene dewaxing.

Hi Richard, I have attached two papers that might be useful Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainswor

RE: [Histonet] Histonet mailing list submission: Jones' Methenamine Silver Stain

hydrazine group (-NHNH2) of thiosemicarbazide blocks the aldehyde group produced following periodic aid treatment and the thiocarbamyl group (-SCNH2) of thiosemicarbazide reduces the methenamine silver. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager

[Histonet] RE: tol blue and mast cells

Hi Richard, I would strongly recommend the Churukian and Schenk method (J.Histotechnol 4(2):85-86, 1981). A simple but brilliant stain. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845

RE: [Histonet] Bone decalcifying/processing problem

disappear. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead

RE: [Histonet] COX/SDH Combo staining

han D, Hrapchak B. (1987). Histotechnology, 2nd Ed. Batelle Press, Columbus p306-307 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road

[Histonet] RE: Immunohistochemistry TAT

Good as long as the wax has a chance to roll off the slide. We tend to use a drying oven. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr H

[Histonet] RE: H&E staining question

You could try 10 minutes treatment with periodic acid prior to H&E staining (Luna's technique I think) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's ho

[Histonet] RE: Immunohistochemistry TAT

ng" J Histotechnol 36(2):45-50) It is also possible that different waxes (especially in older, "cured" blocks) have different de-waxing requirements. Something to consider! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Sen

[Histonet] RE: Bone marrow blocks

preferred fixative 2. Heat 3% agar in a 60oC oven until molten. 3. Centrifuge specimen and decant supernatant. 4. Add 3-4 drops molten agar (dependent on size of sample), gently mix 5. Allow to cool, add fixative, remove block and process. Regards Tony Henwood JP, MSc, BAppSc

RE: [Histonet] Blade Rationing Follow-up

Good Answer!!! ;) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Wes

RE: [Histonet] Shandon Excelsior Tissue Processor

Vanessa, We installed an Eaton 9130 UPS unit with a SSFIO Surge Protector. Cost under $1,500 Australian. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's ho

RE: [Histonet] fungal stain for frozen sections

t; J Clin Microbiol 22(5):803-807. Henwood, A. F., Prasad, L., & Bourke, V. M. (2013). "The application of heated detergent dewaxing and rehydration to techniques for the demonstration of fungi: a comparison to routine xylene-alcohol dewaxing" J Histotechnol, early online DOI 10.1179/

[Histonet] RE: Bone marrow aspirate iron stain

Hi Jason, I am not familiar with the stainer but is the procedure starting from water (not dewaxing & re-hydration as is used for paraffin sections)? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306

RE: [Histonet] Happy 79th Birthday Jules Elias

Well done Jules. Jules visited us "down-under" a few years ago as a guest lecturer at our Scientific Meeting and what an entertaining and knowledgeable presenter he was. It is great to know he is still kicking-up his heels. A great leader of our profession. Regards Tony Henwo

[Histonet] RE: Calcium Stains?

as barium, aluminium, mercury and magnesium. Fortunately, the bright red colouration of calcium can be easily differentiated from the dark red of other metal-lakes. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306

RE: [Histonet] RE: Pituitary gland

o: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Pituitary gland In our library all of these are still known as /Stain Technology/. The name change to /Biotechnic and Histochemistry/ happened much later. Geoff On 4/17/2013 9:14 PM, Tony Henwood (SCHN) wrote: > Some references tha

RE: [Histonet] RE: Pituitary gland

Oh I am getting too old (and I was trying to be modern!) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainswor

[Histonet] RE: Pituitary gland

46. Elftman, H. (1959). Combined aldehyde-fuchsin and periodic acid-Schiff staining of the pituitary. Biotechnic & Histochemistry, 34(2), 77-80. Read More: http://informahealthcare.com/doi/abs/10.3109/10520295909114648 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FF

[Histonet] RE: yeast comtamination on surepath

-dried and stained with Giemsa or DiffQuik, taken from different lots of the un-used collection vials as a QC. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's ho

RE: [Histonet] Re: Histonet Digest, Vol 112, Issue 13

Marilyn' Dandruff is believed to be the main culprit (a gem from the late (and Great) Lee Luna - we miss him). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children'

RE: [Histonet] SMC Immuno and Elastin combo?

Green stock solution5 drops Pour Picric Acid into a conical flask. Add Light Green solution, stir until dissolved. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager &am

RE: [Histonet] Kappa/Lambda ISH

Yep, Sure do. We use a multi-block of appendix (rich in plasma cells). Using the Bond 3, we put the test section on the same slide as the control. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax:

RE: [Histonet] Safranin O staining

s, by Yamamoto et al (1985) Am J Clin Pathol 83:409-414). Interstitial glycosaminoglycans and other mucins are also water soluble so fixing in formal alcohol should retain more of these "mucins" than aqueous NBF fixation (Nathan, & van Deth (1983) Pathology 15:301-4) Regards Tony Henw

[Histonet] RE: "Vacuole" appearance in lymph Nodes

crons will clear the holes (if this is indeed the cause of the holes) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hains

RE: [Histonet] standard protocol

You can forward my reply on to them if you so wish. Here ends my Friday rant!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawke

[Histonet] Expiration dates of ammonium sulphide

! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145,

[Histonet] RE: cytoprep

ice a Unified Protocol Offering the Flexibility of Choosing a Variety of Staining Methods" Acta Cytol 2001; 45:60-68 Ganesan Sivaraman and Krishnan R. Iyengar "Rehydrated Air-Dried Pap Smears as an Alternative to Wet-Fixed Smears" Acta Cytol 2002; 46:713-717 Regards Tony Henwood JP,

[Histonet] RE: ATPase without barbital buffer... info

Hi Tim, Well done Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Wes

[Histonet] RE: cytoprep

The saline re-hydration works well as long as smears are not allowed to dry too long before rehydration. I have found that air-drying longer than 2 hours decreases the effectiveness of the procedure. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory

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