du
Subject: [Histonet] Help to interview new employees for the first time
I am going to interview people for Histotech position for the first time...what
are the best questions to ask? How do I prepare myself? what is the I need to
know that they are the best one? What should I ask or choose? Is g
s.utsouthwestern.edu
> Subject: [Histonet] Help to interview new employees for the first time
>
> I am going to interview people for Histotech position for the first
> time...what are the best questions to ask? How do I prepare myself? what is
> the I need to know that they are the bes
, 2019 11:48 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Help to interview new employees for the first time
I am going to interview people for Histotech position for the first time...what
are the best questions to ask? How do I prepare myself? what is the I need to
know
Hi Blanca,
I think practical skills tests are a great idea. BUT I also think you need
to make sure that having someone that isn't an employee doing tasks in the
lab is a responsibility your lab would willingly take on if something were
to happen. If the candidate cut themselves and was litigious
I am going to interview people for Histotech position for the first time...what
are the best questions to ask? How do I prepare myself? what is the I need to
know that they are the best one? What should I ask or choose? Is good to put
them in action like cutting or staining to check on their
Hi all,
Can someone please provide me with more details regarding cryosectioning of
mouse spleen and liver tissue?
Currently, I've been fixing my samples in 4% PFA (they are well fixed - I
know that will be the first question asked!), and then cryoprotect the
tissue in a series of graded sucrose
On Date: Mon, 23 Jul 2018 18:31:48 -0400
From: Mary Ann
Subject: [Histonet] Positive PAP
Mary Ann wrote "Help! My pathologist has asked that a positive patient be run
down with our PAP stain for QC.
Point me to a reference to counter this request."
Hi Mary Ann - First of all, my sympathies.
Hello Histonet-Members
I am recently asked from my lab-coordinator to establish cutting-grinding
techniques with the technovit-resins for sectioning bones and other
hard-tissue materials.
I do not have many experiences in these techniques and I am looking for
some help. And also, where can I buy
Jenn,
Trying to fully understand. You need someone to prepare these slides again
for you? Great quality work, you provide the specimen? Let me know, I
would love to learn more.
Sincerely,
JB
On Wed, May 31, 2017, 9:13 AM Dearolf, Jenn via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
Hello!
My name is Jenn Dearolf, and I am a professor in the Biology Department at
Hendrix College, a small liberal arts college in Conway, AR. I have written
this list before to get advice about how to prevent freezing artifact in small
muscle samples (Thanks!), but today, I have a completely
Good morning Histoneters!
I am having a problem with eosin staining getting washed away in the upgrades.
Eosin was prepared in isopropyl alcohol, followed one of the
protocols online.
Please advice.
Thank you!
indu
-
: Re: [Histonet] help!!
Tim and Tony,
Why couldn't DAB be used on frozen sections in your example?
Allan
On Mon, Apr 17, 2017 at 9:03 PM, Tony Henwood (SCHN) via Histonet
<histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>>
wrote:
Hi Bianca,
Well for most Patho
tment
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
> -Original Message-
> From: Blanca Lopez via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Thursday, 13 April 2017 1
Subject: [Histonet] help!!
Hello!
I just need a help with a simple question...Is anyone can explain me what is
the purpose between performing immunohistochemistry and Immunofluorescence?
Thanks :)
Blanca Lopez
Histotech (ASCP)
UTSW Tissue Resource K1.210
Simmons Comprehensive Cancer Center
UT
Blanca,
Here are my feelings on this, and I am sure a lot of other folks have feels
here too, so please chime in.
1 - I feel that most clinical labs are more on the IHC bandwagon and
research labs are IF (with the exception of IgG staining in kidney biopsies
or bullous disease in skin- which is
ists.utsouthwestern.edu]
Sent: Thursday, April 13, 2017 6:10 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] help!!
Hello!
I just need a help with a simple question...Is anyone can explain me what is
the purpose between performing immunohistochemistry and Immunofluorescence?
Thanks :)
Hello!
I just need a help with a simple question...Is anyone can explain me what is
the purpose between performing immunohistochemistry and Immunofluorescence?
Thanks :)
Blanca Lopez
Histotech (ASCP)
UTSW Tissue Resource K1.210
Simmons Comprehensive Cancer Center
UT Southwestern Medical Center
Hello All:
I recently acquired a CryoJane unit but it doesn't seem to work in regards to
bone cutting. It works just fine for soft tissue, but getting the bone to
transfer from the tape to the slide just isn't happening. I have tried,
successfully, using other UV sources to transfer the bone
Good afternoon,
I am having a few issues getting nice morphology from both liver and heart
tissues. I currently work only with CNS tissues (brain, spinal cord, DRG,
etc.) but recently, our research team have become interested in
immunostaining some peripheral tissues, including the heart and
Hey everyone,
I know that this question has been asked before and I have tried to find out
information through the internet, but I am having a difficult time trying to
find any type of sample forms. Would anyone be willing to share their
competency assessment forms/checklists? I am trying
mailto:histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Help with causes or theory behind failure of nuclear
counterstaining after IHC!
Maria,
If the counterstain is good when done before IHC stain and poor after it sounds
like proteins are being extracted during the IHC processing
Maria,
This may sound simplistic but I find that if I have problems such as this the
first thing I do is try a new batch of the staining reagents. If you haven't
had problems before then something must have changed either in your protocols
or your reagents. It could be that your heat retrieval
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Help with causes or theory behind failure of nuclear
counterstaining after IHC!
Happy New Year Everyone,
I'm the lead histologist working in an IHC based research lab focused on early
stages of Alzheimer's disease. We work on paraffin sections
Happy New Year Everyone,
I'm the lead histologist working in an IHC based research lab focused on early
stages
of Alzheimer's disease. We work on paraffin sections processed & cut from
600um celloidin
sections. Including a lot of 60um cellodin sections from whole human
brainstem.
For
HELP:
Hi,
Is there anybody can provide me the price list/structure of the custom IHC
services?
For example, to test 200 tissue slides and get 200 images, how much does it
cost?
Thank you very much in advance,
Have a nice weekend,
Best Regards,
Karen
k...@prosci-inc.com
I'd be very interested in this as well...could you shaere Karen.
Thanks.
C
On Mon, Nov 2, 2015 at 11:43 AM, Karen Cai via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> HELP:
>
>
>
> Hi,
>
> Is there anybody can provide me the price list/structure of the custom IHC
> services?
>
>
>
Hello Fellow Histo techs,
This is my first time posting to Histonet. I am looking for experienced
individuals to provide me with a quote (very short 1-2 sentences) on what you
feel has changed most in histology over the years. I am presenting at the MSH
fall symposium and as part of my
Hello All,
I need help -- is anyone using PowerPath with the Amp module for barcode and
driving slide label production? I am hoping for a 5-10 minute conversation
on your workflow, your worksheets, what is working and where you've had to
come up with solutions (where it doesn't work as well as
Dear Colleagues,
You are all well aware of the reproducibility issues when using antibodies for
IHC. I'm working with the Global Biological Standards Institute (GBSI) to
address two important issues-1) cell culture authentication practices and 2)
research antibodies. I have agreed to help
Hi Kimberly,
Most of what I work with is mouse tissue and I've found the brains to be a bit
tricky because they both hydrate and dry out quickly. I normally keep the
paraffin blocks on an ice for about 2 hours (after they've been fully faced
in), checking periodically to make sure the tissue
?Hello Histo folks
I am starting a research project with mouse brain, I am having trouble with
chatter on the regular 3.5 mm sections and cant get the 9-10 mm to cut at all.
I have soaked them in warm water and wonder in using a softener like
conditioner would help. I am new to the
--
*From:* Patsy Ruegg [prueg...@hotmail.com]
*Sent:* Sunday, January 04, 2015 7:03 PM
*To:* Jay Lundgren; Maria Mejia
*Cc:* Histonet@Lists. Edu; Mejia, Mary
*Subject:* RE: [Histonet] HELP! Need some old fashioned histology advice
I have done something similar
, January 04, 2015 7:03 PM
To: Jay Lundgren; Maria Mejia
Cc: Histonet@Lists. Edu; Mejia, Mary
Subject: RE: [Histonet] HELP! Need some old fashioned histology advice
I have done something similar to this but I used tissue that was fixed but not
processed and embedded, this is called enblock labeling, I
I can help with the old fashioned advice:
- 1 scant teaspoon simple syrup
- 2 dashes Angostura Bitters, plus more to taste
- 1 half dollar–sized slice orange peel, including pith
- 2 ounces good-quality rye or bourbon
- 1 maraschino cherry
As for the Histology, is there any
: Sun, 4 Jan 2015 11:58:38 -0600
From: jaylundg...@gmail.com
To: mbmph...@gmail.com
Subject: Re: [Histonet] HELP! Need some old fashioned histology advice
CC: histonet@lists.utsouthwestern.edu; mary.me...@ucsf.edu
I can help with the old fashioned advice:
- 1 scant teaspoon simple syrup
First, the very best of holidays to everyone.
Now for the histology part. Our lab's focus is on the early stages of
Alzheimer's Disease in the Brainstem
using celloidin processing embedding for IHC staining. This year, our lab
will be receiving 6 post-mortem
whole human brains (1 every
Sent from my iPhone
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Hello All,
I hope I am not asking a dumb question. As I know that everyone on the
histonet is very knowledgeable I was hoping to get some suggestions
regarding processing tissue for neuromuscular junction staining. We are a
neuro research lab and want to quantify neuromuscular junction on
Hello All,
Does anyone have a protocol for the Anti-Neutrophil antibody [NIMP-R14]
(ab2557) for mouse tissue? I have been trying to extinguish the background
staining but cannot find the right combination.
We are doing this by hand.
The recommended dilution for this antibody is 1:50 with
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hans B Snyder
Sent: Thursday, November 06, 2014 10:31 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HELP
Hello All,
Does anyone have a protocol for the Anti-Neutrophil antibody [NIMP-R14
Of Elizabeth
Chlipala
Sent: Thursday, November 06, 2014 12:41 PM
To: Hans B Snyder; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] HELP
Hans
We use the antibody from Serotec, it works quite nicely. MCA711, proteinase K
digestion, rabbit anti-rat secondary and then Envision Rabbit
Hi,
I'm having extreme difficulty finding someone who can work on the old IEC
CTDs in Southern California. The issue isn't with the microtome, it's with
the compressor/refrigeration. I've had a few people tell me they deal with
these machines, only to find out after many hours of labor that
I am in Indiana - I need a Leica sales rep to contact me please or if someone
can provide me with a regional sales rep name - that would be awesome!
Thanks!
Kristie
Ph 812-307-2093
christina.wo...@bms.com
This message (including any attachments) may contain
Hi all,
I've been having problems with cryosectioning fatty tissue for a long time now.
The section leaves a hole in the middle. I know this is probably a much
discussed topic, but I've tried a lot of strategies with no luck. The tissue is
of mouse origin and has micro-lesions buried inside
Thanks Sandra! My next step was to try that.
Dakshna
- Original Message -
From: Sandra E. Esparza sespa...@seton.org
To: Dakshnapriya Balasubbramanian dakshnapr...@neo.tamu.edu
Sent: Tuesday, May 20, 2014 10:34:25 AM
Subject: RE: [Histonet] HELP- Cryosectioning FAT!
You might want
To: Dakshnapriya Balasubbramanian dakshnapr...@neo.tamu.edu
Sent: Tuesday, May 20, 2014 10:37:35 AM
Subject: RE: [Histonet] HELP- Cryosectioning FAT!
Dakshna,
Has this tissue been fixed before freezing by any chance?
laurie
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Hi Rui,
You uploaded a tif image and a number of browsers don't support tif
images. Jpeg, gif and png images are the best image formats to use
because they are universally supported. I converted your image to a jpeg
image and posted it at:
: Re: [Histonet] Help for identifying the blue stained structure
Hi Rui,
You uploaded a tif image and a number of browsers don't support tif
images. Jpeg, gif and png images are the best image formats to use
because they are universally supported. I converted
Hello,
I have an embryonic sample that
decalcified, paraffin embedded, and stained with Mallory Trichrome (Aniline
Blue, Orange G, Acid Fuchsin). I will upload the image in the Histonet Images.
This
is a cranial region where the bone is being resorbed, so I expected to see the
bone (dark blue
Our lab has started using Immunocal decal solution on our bone marrows. Most of
our antibodies have improved except kappa and lambda. We do not do ISH. Kappa
and lambda staining in the tonsil controls is good, The bone marrows are now
very overstrained. We use DAKO polyclonals at 1:10,000
Hi Histonetters,
Would you please let me know why I can not get PDF file from J
Histotechnology any more?
I renewed my membership after new year and changed my password since i
don't remember my old one. I can get in Maney Online for only Abstract,
but not whole article.
The paper I tried to
[mailto:
histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dorothy Hu
Sent: Friday, January 17, 2014 8:57 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Help on get PDF file paper from J. Histotechnology
Hi Histonetters,
Would you please let me know why I can not get PDF file
FACEPALM.JPG
this is not even worth an actual jpg.
sigh.
By bitching and bitching and bitching, they could exhaust the drama of
their own horror stories. Grow bored. Only then could they accept a new
story for their lives. Move forward.
-Chuck Palahniuk, Haunted
On Wed, Sep 11, 2013 at 7:51
Get me off your mailing list.
I have tried and keep getting these things.
Over and over you repeat the same questions and answers , over and over,
over and over, over and over, over and over.
Do you get the message?
Do you get the message?
Do you get the message?
Get me off
Googling 'unsubscribe histonet' points me to this link:
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
If you scroll to the bottom of the page you will find a box where you can
type your e-mail address to unsubscribe or edit options. Have you tried
that?
Cheers.
--
Virginia Bain
lol
On Wed, Sep 11, 2013 at 5:56 PM, Bain,Virginia veb...@mdanderson.orgwrote:
Googling 'unsubscribe histonet' points me to this link:
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
If you scroll to the bottom of the page you will find a box where you can
type your e-mail
Merritt College has started a HT program and is in need of clinical sites
for their students to get hands-on experience. If you interested in
being a mentor for the HT students please contact Gisele Giorgi at
profgio...@peralta.edu
___
Histonet
Hey everyone.
I Need some help with my processor. It is a thermo scientific stp 120. I
came in today and when I went to puch the button to shut the alarm off, I
was shocked. The machine shocked me and then the little green display
screen went blank. I managed to get my blocks off the machine and I
I need to speak with someone who has experience with doing (and interpretating)
in situ hybridization for EBER. Please contact me directly. Thank you.
Richard
Richard W. Cartun, MS, PhD
Director, Histology Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic
...@lists.utsouthwestern.edu] On Behalf Of Natalie Nagy
Sent: Thursday, January 10, 2013 10:36 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Help with CPT code 88363 for archived tissue retrieval
Hi everyone,
I just have a question about CPT code 88363, first can it be
used
Hi everyone,
I just have a question about CPT code 88363, first can it be
used for pulling blocks for Oncotype DX testing, also is there a time limit on
when this code can be used? Does it have to be within a year, a month, etc...of
when the patient account went active?
It can't be used to just pull blocks. The slides have to be reviewed and
the best block chosen by a pathologist. If there is only one block then
the pathologist needs to look at the slides and determine if there is
enough tissue for molecular testing. It's a professional charge.
Use it on
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango
Sent: Thursday, January 10, 2013 2:01 PM
To: Natalie Nagy
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Help with CPT code 88363 for archived tissue retrieval
It can't be used to just pull blocks
: Re: [Histonet] Help with CPT code 88363 for archived tissue retrieval
It can't be used to just pull blocks. The slides have to be reviewed and the
best block chosen by a pathologist. If there is only one block then the
pathologist needs to look at the slides and determine if there is enough
PM
To: 'Mark Tarango'; Natalie Nagy
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Help with CPT code 88363 for archived tissue
retrieval
And I should explain the reason I most know this.. our pathologists were
denied because the tech charge hadn't been entered yet. So now I make
@lists.utsouthwestern.edu
Subject: RE: [Histonet] Help with CPT code 88363 for archived tissue retrieval
So are you putting the charge thru twice or is the charge for the 88363 divided
out into tech and prof.?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun
...@lists.utsouthwestern.edu] On Behalf Of Weems,
Joyce K.
Sent: Thursday, January 10, 2013 1:06 PM
To: 'Mark Tarango'; Natalie Nagy
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Help with CPT code 88363 for archived tissue
retrieval
And I should explain the reason I most know this.. our pathologists
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun
Sent: Thursday, January 10, 2013 2:47 PM
To: Joyce K. Weems; Mark Tarango; Mike Pence; Natalie Nagy
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Help with CPT code 88363 for archived
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Thursday, January 10, 2013 1:06 PM
To: 'Mark Tarango'; Natalie Nagy
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Help with CPT code 88363 for archived tissue retrieval
And I should
To: 'Bell, Lynne'; 'Richard Cartun'; Weems, Joyce K.; 'Mark Tarango'; 'Mike
Pence'; 'Natalie Nagy'
Cc: 'histonet@lists.utsouthwestern.edu'
Subject: RE: [Histonet] Help with CPT code 88363 for archived tissue retrieval
We have been billing a tech and pro fee since 2011. Whether we are getting
reimbursed
I want to be remove from the list. I've tried the unsubscribe link be low
several time but it seem like it doesn't work because I'm still receiving
daily email
On Friday, December 28, 2012, wrote:
Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu
To
Hi Ly,
When you click on the Unsubscribe button, an email is sent to you to
confirm you want to unsubscribe. It has a link to click to confirm you
want to unsubscribe. Is the confirmation email getting lost in your junk
folder? You won't be unsubscribed until you click the link in the
: [Histonet] Help
Hi Ly,
When you click on the Unsubscribe button, an email is sent to you to confirm
you want to unsubscribe. It has a link to click to confirm you want to
unsubscribe. Is the confirmation email getting lost in your junk folder? You
won't be unsubscribed until you click the link
] On Behalf Of Marvin Hanna
Sent: Friday, December 28, 2012 2:28 PM
To: histonet@lists.utsouthwestern.edu; ln0...@gmail.com
Subject: Re: [Histonet] Help
Hi Ly,
When you click on the Unsubscribe button, an email is sent to you to confirm you want to
unsubscribe. It has a link to click to confirm
Is there an alternative to toluidine blue stain for plastic embedded tissue?
I have some valuable epoxy resin embedded rat spinal cord blocks that were not
well osmicated. Toluidine Blue staining of the semi-thin sections is uneven
and pale in the center of the sections. Is there another stain
: [Histonet] Help! Liver mistakenly processed in paraffin (had
tobe in OCT instead)!
It depends on what you are using the oil red o for. Lipofuscin and ceroid
can be demonstrated with an oil red o stain after processing.
Jennifer MacDonald
From: z o n k e d zon...@gmail.com
To: histonet
Hello Histonetters,
First time writer, long time reader. I'm a newbie tech in academia and I
was given a simple task which I think I pretty much screwed up.
I should have embedded half of a mouse liver in paraffin for microtome
sectioning while the other half should have been embedded in OCT for
Nope, sorry. All your fat is dissolved.
Sent from my iPhone
On Nov 13, 2012, at 8:52 AM, z o n k e d zon...@gmail.com wrote:
Hello Histonetters,
First time writer, long time reader. I'm a newbie tech in academia and I
was given a simple task which I think I pretty much screwed up.
I
Oil Red O is a stain for fat, Alcohol dissolves fat Rehydrating won't
help. You can't replace the fat.
Rena Fail
On Tue, Nov 13, 2012 at 11:52 AM, z o n k e d zon...@gmail.com wrote:
Hello Histonetters,
First time writer, long time reader. I'm a newbie tech in academia and I
was given a
:53 AM
Subject:[Histonet] Help! Liver mistakenly processed in paraffin
(had to be in OCT instead)!
Sent by:histonet-boun...@lists.utsouthwestern.edu
Hello Histonetters,
First time writer, long time reader. I'm a newbie tech in academia and I
was given a simple task which I
Subject: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in
OCT instead)!
Hello Histonetters,
First time writer, long time reader. I'm a newbie tech in academia and I
was given a simple task which I think I pretty much screwed up.
I should have embedded half of a mouse liver
Good morning everyone,
Does anyone have a protocol they use to demonstrate dead fungi in FFPPE tissue?
Thanks!
Jeanine H. Bartlett, BS HT(ASCP), QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, NE
MS/G-32
Atlanta, Ga 30333
404-639-3590
@lists.utsouthwestern.edu
Sent: Thursday, October 11, 2012 7:53 AM
Subject: [Histonet] help with dead fungi
Good morning everyone,
Does anyone have a protocol they use to demonstrate dead fungi in FFPPE tissue?
Thanks!
Jeanine H. Bartlett, BS HT(ASCP), QIHC
Centers for Disease Control and Prevention
Hello dear histoneters
First let me thank you all for your help for lost password (autostainer
plus software) - a - a tip is ok.
I am begining to test the autostainer and now eveything seems fine
(there was a stuck tube but I could settle the problem)
_Corrupted files : Dako Seymour
Hello
I just bought a second hand Dako autostainer from Germany (I live in
France) from a broker.
The machine looks fine but I can't test it because_I don't have identity
and password _to get inside the software.
Does anyone know how if it is possible to localise the password and
identities
11:45 PM
Subject: [Histonet] help ! paraffin section
Hi All
I am trying to section adult mouse intestine and skin using paraffin
embedding. However, when i section, the tissue is torn although the rest of
the paraffin looks perfect. Please suggest why this is happening. Also,
sometimes the skin
In addition to Rene's comment,to cut coagulated tissue (skin that have
new wound crust) and calcified tissue is difficult.
On Wed, Aug 8, 2012 at 6:45 AM, Megha Kumar meg...@g.clemson.edu wrote:
Hi All
I am trying to section adult mouse intestine and skin using paraffin
embedding.
Hi All
I am trying to section adult mouse intestine and skin using paraffin
embedding. However, when i section, the tissue is torn although the rest of
the paraffin looks perfect. Please suggest why this is happening. Also,
sometimes the skin sections fall off the slides when I perform in situ
To:
'histonet@lists.utsouthwestern.edu'histonet@lists.utsouthwestern.edu
Subject: [Histonet] Help
I'm a Histotechnologist working in the Regional Hospital in Barrie,
ON Canada. We are using the Ventana Ultra for our Immunohistochemistry
(IHC). Since the end of February, we have been having issues
I realize that such + slides come with the instruction to completely
dry slides at room temperature before placing in the drying oven. I
have used these slides for many years, and have found this procedure to
be not only unnecessary, but sometimes problematic. I believe sections
are more likely
I'm a Histotechnologist working in the Regional Hospital in Barrie, ON Canada.
We are using the Ventana Ultra for our Immunohistochemistry (IHC). Since the
end of February, we have been having issues with some tissues lifting off our
positive (marked with +) charged slides. It seems to be
modify a concentration
with dilution, not with time. Perhaps you could modify the HIER step, or try to
dilute the antibody.
René J.
--- On Fri, 5/18/12, Cloughley-Gray, Nancy cloughl...@rvh.on.ca wrote:
From: Cloughley-Gray, Nancy cloughl...@rvh.on.ca
Subject: [Histonet] Help
To: 'histonet
Hello All,
I am about to start working on my MS thesis and working on ground section of
the mandible with some implants and HA particulates , so I need some help to
tap into some of your knowledge on TRAP staining for osteoclasts. I would
appreciate if I can get any help with a TRAP staining
Koelling
PhenoPath Labs
Seattle, WA
- Original Message -
From: Lydia Gunawan lydia.guna...@unimelb.edu.au
To: Histonet@lists.utsouthwestern.edu
Sent: Tuesday, November 15, 2011 2:00:32 PM
Subject: [Histonet] help
Hi there, I am having trouble with Turnbull staining. Anybody can
, November 15, 2011 2:00:32 PM
Subject: [Histonet] help
Hi there, I am having trouble with Turnbull staining. Anybody can help me?
As I want to detect and quantify iron in the brain for Parkinson experiment, I
am using mice(C57Bl6) brain that were treated with MPTP injection. So, I have
been
Lydia Gunawan
Oxidation Biology Laboratory
Mental Health Research Institute
Melbourne Brain Centre
Corner Royal Pde and Genetics Lane
University of Melbourne, Level 4
Parkville, Vic 3010
email: lydia.guna...@unimelb.edu.au
___
Histonet mailing list
Hi there, I am having trouble with Turnbull staining. Anybody can help me?
As I want to detect and quantify iron in the brain for Parkinson experiment, I
am using mice(C57Bl6) brain that were treated with MPTP injection. So, I have
been trying to stain those brain using paraffin section with
I am currently a histology student at Keiser University. I am doing a project
for my routine staining class about Celestine Blue. I've been able to find
information on why it was created, the chemical make up, and some of it's uses
including the trichrome stain. I am having trouble finding
Hello All,
This is my first time posting a request. We use metal embedding molds,
and would like to order one particular size. The outer measurements are
2.5mm x 2.5mm x
0.6 mm. We are wondering if anyone has approximately 40-50 of them we
could purchase-new or used. The reason we need this size
Hello,
I am completely stumped by a problem we¹ve been having with our Masson¹s
Trichrome. It isn¹t working properly on cryo sections. We developed our
protocol several years ago and it has been a standard in our lab without any
trouble, until about four months ago when it just stopped working
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