[Histonet] Help

I am currently a histology student at Keiser University. I am doing a project for my routine staining class about Celestine Blue. I've been able to find information on why it was created, the chemical make up, and some of it's uses including the trichrome stain. I am having trouble finding i

[Histonet] Help finding metal embedding molds

Hello All, This is my first time posting a request. We use metal embedding molds, and would like to order one particular size. The outer measurements are 2.5mm x 2.5mm x 0.6 mm. We are wondering if anyone has approximately 40-50 of them we could purchase-new or used. The reason we need this size i

RE: [Histonet] Help with Masson's Trichrome - not working on cryo sections

2011 2:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help with Masson's Trichrome - not working on cryo sections Hello, I am completely stumped by a problem we¹ve been having with our Masson¹s Trichrome. It isn¹t working properly on cryo sections. We developed our pr

[Histonet] Help with Masson's Trichrome - not working on cryo sections

Hello, I am completely stumped by a problem we¹ve been having with our Masson¹s Trichrome. It isn¹t working properly on cryo sections. We developed our protocol several years ago and it has been a standard in our lab without any trouble, until about four months ago when it just stopped working o

[Histonet] Help me again please

What dilution do you guys use on a rabbit polyclonal Ki-67 antibody...I just did 1/500 and got a crap ton of noise in the areas where the positive staining was. There are definite negative "rivers" flowing through the samples, but even the tonsil control has this same fuzziness to it. Any suggest

RE: [Histonet] [Help] oil red O stain in fattty change liver

#x27;s Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kyoung LEE Sent: 14 April 2011 15:07 To: histonet@lists.utsouthwestern.edu Subject: [

Re: [Histonet] [Help] oil red O stain in fatty change liver

water to remove the OCT and then proceed with the stain. kyoung LEE Sent by: histonet-boun...@lists.utsouthwestern.edu 04/14/2011 08:04 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] [Help] oil red O stain in fattty change liver Dear sirs. I don't know whether I

RE: [Histonet] [Help] oil red O stain in fattty change liver

...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kyoung LEE Sent: Thursday, April 14, 2011 9:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] [Help] oil red O stain in fattty change liver Dear sirs. I don't know whether I send this question to you. For se

[Histonet] [Help] oil red O stain in fattty change liver

Dear sirs. I don't know whether I send this question to you. For several weeks, I'm setting oil red O stain in fattty change liver (mouse). Could you review my protocol? 1. Murine tissue,livers, is fixed with 10% NBF(10% neutral-buffered formalin). 2. Make frozen block 3. Blocks were cut 5um i

RE: [Histonet] HELP!!!!! H. Pylori Immunos

From: Jay Lundgren [jaylundg...@gmail.com] Sent: Tuesday, March 01, 2011 3:10 PM To: McMahon, Loralee A Cc: Heather Cooper; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HELP! H. Pylori Immunos Our problem, in my opinion is a control issue and not a staining one..our

Re: [Histonet] HELP!!!!! H. Pylori Immunos

Our problem, in my opinion is a control issue and not a staining one..our pathologist wants to see the bugs in certain areas-glands(spelling appologies!) its called, the circular groups of cells, in the middle of these areas and not just occasional bugs in other areasEverywhere else I've worked

Re: [Histonet] HELP!!!!! H. Pylori Immunos

I've done them by hand, what's your question? ??? Jay A. Lundgren M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http:/

RE: [Histonet] HELP!!!!! H. Pylori Immunos

...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heather Cooper [hctrup...@att.net] Sent: Tuesday, March 01, 2011 12:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HELP! H. Pylori Immunos Does anyone do H. Pylori Immunos by hand

[Histonet] HELP!!!!! H. Pylori Immunos

Does anyone do H. Pylori Immunos by hand? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Help with OCT problem

tonet-boun...@lists.utsouthwestern.edu on behalf of Liz Chlipala Sent: Thu 2/10/2011 1:49 PM To: Reynolds,Donna M; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help with OCT problem Donna I have seen soft or rather sticky OCT samples in the past. If you freeze in isopentane and don&

Re: [Histonet] Help with OCT problem

I agree with previous responders to this query, as I've gotten alcohol on my blocks and since alcohol doesn't freeze real well it makes a sticky mess with the OCT. The affected OCT can be scraped off with a razor blade. Hopefully the tissue itself did not come in contact with alcohol prior to e

Re: [Histonet] Help with OCT problem

Donna, I would also check to make sure that the tissue wasn't held in an something with a lot of alcohol, like RNA later. I found out first hand that it doesn't work well. Good luck On Thu, Feb 10, 2011 at 11:38 AM, Reynolds,Donna M wrote: > Has anyone ever experienced OCT blocks that cut like

RE: [Histonet] Help with OCT problem

-boun...@lists.utsouthwestern.edu] On Behalf Of Reynolds,Donna M Sent: Thursday, February 10, 2011 12:39 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Help with OCT problem Has anyone ever experienced OCT blocks that cut like they are soft. I have turned the cyrostat down 1

[Histonet] Help with OCT problem

Has anyone ever experienced OCT blocks that cut like they are soft. I have turned the cyrostat down 10 degrees lower than usual and they are still soft. Really weird part is that I have 20 blocks in this group and some are soft and others are just fine. Tried changing angle, new blade nothing he

Re: [Histonet] Help

athy Nelson To: Histonet Sent: Sun, December 19, 2010 1:25:54 AM Subject: [Histonet] Help Solutions for cracks in tissue microscopically esp. in tumors and BCC specimens. Thanks kathyenel...@hotmail.com                         ___ Histonet mailin

[Histonet] Help

Solutions for cracks in tissue microscopically esp. in tumors and BCC specimens. Thanks kathyenel...@hotmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailma

[Histonet] Help cutting horse tendon

I am new to the list so I don't know your experience. If your spaces are too wide did you cryoprotect before freezing? As freezing when done right gives very consistent size retention. Alex ___ Histonet mailing list Histonet@lists.utsouthwestern.edu ht

[Histonet] Help cutting horse tendon.

Dear listers, I am trying to cut whole sections of the tendon of the digital flexor muscle of the horse. The piece is around 1 mm long and very elastic and fibrous. I have tried formalin and Bouin fixation, the latest better. With paraffin embedding the fibrous elements of the tendon spoil th

Re: [Histonet] Help! Curled cryosections

Thanks, Montina The sections are 40um and I had no trouble with all my other brains (sectioned myself). I had help with these last two and they are all curled. I think they just got sectioned too fast or something. I'll try the shaker and see if it helps. Thanks! -Teresa On Wed, Sep 15, 2010 at

[Histonet] Help! Curled cryosections

Is there any way to relax curled brain sections? They've been in > cryoprotectant and I tried sitting them in PBS for a while. They're to be > used in an IHC- can they be rescued? > > > >> Thanks, >> -- >> Teresa Iglesias >> Graduate Group in Animal Behavior >> University of California-Davis >> >>

[Histonet] Help with Biocare polymer detection kit!!

I have been trying to get the Mach 1 polymer kit from Biocare to work on my K9 and feline tissue and I am getting terrible nonspecific staining in my positive and negative controls. It appears to be sticking to something in the glands of the intestinal epithelia. I am also getting nonspecific

RE: [Histonet] Help Resurrecting Dried Fixed Tissue

n.edu] On Behalf Of Parsons, Catherine Sent: Thursday, 24 June 2010 1:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help Resurrecting Dried Fixed Tissue I am grasping at straws and hoping for ideas to salvage some formalin fixed tissue specimens. I work in a research lab,

RE: [Histonet] Help! In need of positive Gram Control

tonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help! In need of positive Gram Control You have got to be kidding!! That's hysterical. So process a slim jim and you have Gram - and + controls. If you're serious I'm trying it. -Original Message- From: histonet-boun...@list

[Histonet] Help Resurrecting Dried Fixed Tissue

I am grasping at straws and hoping for ideas to salvage some formalin fixed tissue specimens. I work in a research lab, and am quite new to histology and this particular lab. A former student left formalin fixed tissue specimens that were stored in 70% ethanol. Unfortunately, the seal failed

RE: [Histonet] Help! In need of positive Gram Control

] On Behalf Of connie grubaugh Sent: Tuesday, June 22, 2010 9:06 PM To: cg...@marylandgeneral.org; jcbrit...@cheshire-med.com; dianar...@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help! In need of positive Gram Control Tried the slim jim and all of my doctors did not like

RE: [Histonet] Help! In need of positive Gram Control

Tried the slim jim and all of my doctors did not like it. Don't waste your time. Connie G. > Date: Tue, 22 Jun 2010 10:16:14 -0400 > From: cg...@marylandgeneral.org > To: jcbrit...@cheshire-med.com; dianar...@aol.com; > histonet@lists.utsouthwestern.edu > Subject:

Re: [Histonet] Help! In need of positive Gram Control

Slim Jim works great. This is what we used to use at AFIP. Just slice it and process it the same as your regular tissue. Jay A. Lundgren, M.S., HTL (ASCP) Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman

Re: [Histonet] Help! In need of positive Gram Control

A good ol' hot appendix works great. Not as good as a Slim Jim, tho. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Help! In need of positive Gram Control

l.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help! In need of positive Gram Control Have you tried a Slim Jim? They have gram positive and negative rods in them. Regardless, I still enjoy eating them once and a while! Josie Britton Ht Cheshire Medical Center

RE: [Histonet] Help! In need of positive Gram Control

f Josie Britton Sent: Tuesday, June 22, 2010 6:10 AM To: dianar...@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help! In need of positive Gram Control Have you tried a Slim Jim? They have gram positive and negative rods in them. Regardless, I still enjoy eating them o

RE: [Histonet] Help! In need of positive Gram Control

[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of dianar...@aol.com Sent: Monday, June 21, 2010 7:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help! In need of positive Gram Control Help! We are in need of positive Gram Control Blocks if anyone has any extra they are

RE: [Histonet] Help! In need of positive Gram Control

tonet@lists.utsouthwestern.edu Subject: [Histonet] Help! In need of positive Gram Control Help! We are in need of positive Gram Control Blocks if anyone has any extra they are willing to part with. I have lots of Fungus, Pneumocystis and HPV tissue blocks to trade. Diana Ripley John Muir Histology Concord C

[Histonet] Help! In need of positive Gram Control

Help! We are in need of positive Gram Control Blocks if anyone has any extra they are willing to part with. I have lots of Fungus, Pneumocystis and HPV tissue blocks to trade. Diana Ripley John Muir Histology Concord Campus 2540 East Street Concord, CA 94520 _

Re: [Histonet] help

Have you tried sodium thiosulphite? That will leave white spots instead of brown, which is an improvement and easier to cover. Lesley Weston. On 23-Mar-10, at 8:59 AM, Patsy Ruegg wrote: After you stop laughing seriously I need some help here, apparently I got my fingers in some silver nit

re: [Histonet] help

ice facial staining and compliment it with a bit of Nuclear Fast Red or Light Green. Best of luck, Amos Date: Tue, 23 Mar 2010 09:59:52 -0600 From: "Patsy Ruegg" Subject: [Histonet] help To: Message-ID: < 86ece24c976c4fb8950023175ef1e...@patsyoffice> Content-Type: text/plain;

RE: [Histonet] help (UNCLASSIFIED)

v'" cc histonet@lists.utsouthwestern.edu Subject RE: [Histonet] help (UNCLASSIFIED) Are you trying to kill me? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pru...@ihctech.

Re: [Histonet] help

Touch the affected areas with Lugol's solution. After that remove the Lugol with sodium thiosulfate. René J. --- On Tue, 3/23/10, Patsy Ruegg wrote: From: Patsy Ruegg Subject: [Histonet] help To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 23, 2010, 11:59 AM After you

Re: Re[2]: [Histonet] help (UNCLASSIFIED)

cipient may > > constitute a breach of civil or criminal law and is strictly prohibited. > If > > you are NOT the intended recipient please contact the sender and dispose > of > > this e-mail as soon as possible. > > > -----Original Message- > > From: Ma

Re[2]: [Histonet] help (UNCLASSIFIED)

d recipient please contact the sender and dispose of > this e-mail as soon as possible. > -Original Message- > From: Maxim Peshkov [mailto:maxim...@mail.ru] > Sent: Tuesday, March 23, 2010 11:43 AM > To: pru...@ihctech.net > Cc: histonet@lists.utsouthwestern.edu > S

Re: [Histonet] help for silver

;s reducer (ferricyanide + thiosulphate mixture) but my experience is that they do very little. The blackened epidermis flakes off, usually in a couple of days. John Kiernan Anatomy, UWO London, Canada. = = = - Original Message - From: louise renton Date: Tuesday, March 23, 2010 13:58 Subject:

RE: [Histonet] help (UNCLASSIFIED)

sage- From: Maxim Peshkov [mailto:maxim...@mail.ru] Sent: Tuesday, March 23, 2010 11:43 AM To: pru...@ihctech.net Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] help (UNCLASSIFIED) Patsy: Chemically, silver nitrate deposites can be reduced onto the slides by 0.5% potassium ferr

[Histonet] help for silver

I know we used Sodium thiosulphate and alcoholic iodine, but i cannot remember which ne first...I think the iodine?? John Kiernan - help please?? On Tue, Mar 23, 2010 at 5:59 PM, Patsy Ruegg wrote: > After you stop laughing seriously I need some help here, apparently I got > my > fingers in so

RE: [Histonet] help (UNCLASSIFIED)

Patsy: Chemically, silver nitrate deposites can be reduced onto the slides by 0.5% potassium ferricyanide. I am not sure that it may work onto living skin. Sincerely, Maxim Peshkov, Russia, Taganrog. mailto:maxim...@mail.ru ___ Hi

RE: [Histonet] help

Pretty funny stuff!! I say to try the sodium thio - it removes unreduced silver. Or you could grab a Brillo pad and scrub away! This laugh was just what I needed today. Thank you. Lynne A. Bell, HT (ASCP) Technical Specialist, Histology Central Vermont Medical Center 130 Fisher Road Barre, VT

RE: [Histonet] help

If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -Original Message- From: Weems, Joyce [mailto:jwe...@sjha.org] Sent: Tuesday, March 23, 2010 10:08 AM To: Patsy Ruegg; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet]

RE: [Histonet] help (UNCLASSIFIED)

3-751- 2530 FAX: 803-751-7829 DSN:    734-2530 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, March 23, 2010 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [His

RE: [Histonet] help

Basically time -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, March 23, 2010 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] help After you stop laughing

RE: [Histonet] help

0 16:00 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] help After you stop laughing seriously I need some help here, apparently I got my fingers in some silver nitrate yesterday and touched my face under my nose over my lip and now I have black spots that won't come off. I have

Re: [Histonet] help

n.edu Sent: Tue, March 23, 2010 11:09:35 AM Subject: Re: [Histonet] help You could use a sharpie to fill in a killer moustache. I say go with handlebars and not with Hitler-style. Emily Shall we always be content with the ancient tinned salad of the subsidized novel? Or the tired ice-cream of

Re: [Histonet] help

You could use a sharpie to fill in a killer moustache. I say go with handlebars and not with Hitler-style. Emily Shall we always be content with the ancient tinned salad of the subsidized novel? Or the tired ice-cream of poems which cry themselves to sleep in the refrigerators of the mind? -Lawre

Re: [Histonet] help

So sorry... the only thing I know to fix it is time... about a week or two... :) Drew On Tue, Mar 23, 2010 at 11:59, Patsy Ruegg wrote: > After you stop laughing seriously I need some help here, apparently I got > my > fingers in some silver nitrate yesterday and touched my face under my nose >

RE: [Histonet] help

: [Histonet] help After you stop laughing seriously I need some help here, apparently I got my fingers in some silver nitrate yesterday and touched my face under my nose over my lip and now I have black spots that won't come off. I have done this on my hands before but never on my face. So far I

Fw: [Histonet] help

I am so sorry but you just made my day, lol!! Wish I had a remedy for you, sorry.. Jill Cox HT (ASCP) - Forwarded Message From: Patsy Ruegg To: histonet@lists.utsouthwestern.edu Sent: Tue, March 23, 2010 8:59:52 AM Subject: [Histonet] help After you stop laughing

[Histonet] help

After you stop laughing seriously I need some help here, apparently I got my fingers in some silver nitrate yesterday and touched my face under my nose over my lip and now I have black spots that won't come off. I have done this on my hands before but never on my face. So far I have tried soaking

Re: [Histonet] Help...

And what did you do? René J. --- On Thu, 2/11/10, Fawn Bomar wrote: From: Fawn Bomar Subject: [Histonet] Help... To: "histonet@lists.utsouthwestern.edu" Date: Thursday, February 11, 2010, 2:06 PM Does anyone have a protocol or procedure for validating the Her2 antibody?  We are

RE: [Histonet] Help...

, 2010 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help... Does anyone have a protocol or procedure for validating the Her2 antibody? We are trying to get ready for a CAP inspection and I'm not sure if what we did is ok or not. Thank you in advance

[Histonet] Help...

Does anyone have a protocol or procedure for validating the Her2 antibody? We are trying to get ready for a CAP inspection and I'm not sure if what we did is ok or not. Thank you in advance Fawn - This electronic message may contain in

[Histonet] Help with IHC

Hello, I would like to stain FFPE mouse brain tissue with MCP-1 antibody from serotec. I was wondering if anyone who uses this marker could share their protocol and indicate what is a good positive control. Thanks in advance. Michael Valerio University at Buffalo valer...@buffalo.edu 716-829-

Re: [Histonet] Help with Large Eye Specimen

Hi Sara: I am forwarding your e-mail to J. Staruk because I think he can do that type of work. You can also consult him at the included e-mail address. René J. --- On Fri, 12/11/09, Breeden, Sara wrote: From: Breeden, Sara Subject: [Histonet] Help with Large Eye Specimen To: histonet

[Histonet] Help with Large Eye Specimen

I do not have the equipment to do a whole horse eye (with levels) on "lantern" slides. We rarely have call for this and my boss would like to know if there is a vet school or reference lab that could process an entire eye, cut levels (to be determined) to lantern slides. Alternately, if you know a

RE: [Histonet] Help! Losing sections from Superfrost Plus Slides

I would suggest you check the lot#. You may have received a bad lot and need to get them replaced by the vendor. >> > Hi all, > > We have suddenly started losing tissue sections from our Superfrost Plus > Slides. > Our students have been cutting fixed, frozen, cryosections (20um) and > th

[Histonet] Help! Losing sections from Superfrost Plus Slides

Prepare a 1% aq. sol. of "Elmer's Glue" and coat the slides with it. Oven dry and use the slides as usual. René J. --- On Wed, 12/9/09, Angelina Fong wrote: From: Angelina Fong Subject: [Histonet] Help! Losing sections from Superfrost Plus Slides To: "histonet@lists.utsou

[Histonet] Help! Losing sections from Superfrost Plus Slides

Angelina, I have seen this post a few times before and sometimes the problem was the drying technique or making sure your water bath is clean and free of any debris. I also remember hearing about someone pre-treating their slides with a Trilogy (EDTA) buffer in a pressure cooker. Good Luck! *Mat

[Histonet] Help! Losing sections from Superfrost Plus Slides

Hi all, We have suddenly started losing tissue sections from our Superfrost Plus Slides. Our students have been cutting fixed, frozen, cryosections (20um) and thaw-mounting these onto Superfrost Plus slides. We have suddenly started losing lots of sections from these slides (again!!). The

[Histonet] Help needed to calibrate BioGenex Optimax

Is there someone who has done this calibration that would be willing to talk me through calibrating the X-Y calibration for my Optimax Plus stainer? It will not pick up the tips and I have recently installed the software update. It worked fine before that. Email and let me know the

[Histonet] HELP - p16 protocol

Hi everyone, We will soon be purchasing p16 from mtm laboratory. If i just want buy the antibody what is the protocol for using a decloaker chamber and Dako's retrieval solution? The directions say to use a waterbath but we use DC chambers. What temp. can i use and for how long? Any

[Histonet] Help with fixed-frozen tissue sections

Dear Histonet members, for a few years I have done IHC on fixed-frozen mouse embryos with good success. A few days ago I moved onto adult tissues (liver and pancreas) and have met diffulties getting sections of good qualitiy. Here is how I proceeded: - Pancreas and liver were fixed whole, in Zi

[Histonet] Help with Connexin 43

Anyone out there doing FFPE IHC on rat for Connexin 43 - I am having trouble getting the titration right on this & just wondering what types of dilutions anyone is using??? I am using a standard avidin/biotin complex staining system with and endogenous AV/BI block and not getting anything of an

RE: [Histonet] HELP Starting a lab

Pretty good list. Don't forget first aid kits and spill kits. Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Mon, 20 Jul 2009 14:26:57 -0400 > From: ktut...@umm.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] HELP Star

[Histonet] HELP Starting a lab

I have a friend who is a Pathologist and starting a new lab. She sent me a list of start up supplies and equipment that she needs to purchase. Can I get some input on whats missing from her list, I've added a few things but Im going blank. Its a hisology lab for a urology practice if that helps.

Re: [Histonet] Help With Hemo Fading

to: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: 02 July 2009 20:28 > To: histonet@lists.utsouthwestern.edu; sr...@aol.com > Subject: Re: [Histonet] Help With Hemo Fading > > The fading most probably is caused by acid in the permanent slide, pr

RE: [Histonet] Help With Hemo Fading

tonet@lists.utsouthwestern.edu; sr...@aol.com Subject: Re: [Histonet] Help With Hemo Fading The fading most probably is caused by acid in the permanent slide, probably because the sections were passed through the alcohols very quickly after the acid differentiation, or they stayed little time in tap

RE: [Histonet] Help With Hemo Fading

!!! Beware the resolve of an elected President not to lose. Frederick C. Monson, PhD Technical Director, CMIRT West Chester University West Chester, PA, 19383 610-738-0437 http://cmirt.wcupa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu on behalf of Rene J B

Re: [Histonet] Help With Hemo Fading

mounting medium is acidic, although that could also be the cause also. An acid environment over the cover slipped section is the most probable "culprit" for the henatoxylin fading. Check the staining protocol. René J. --- On Thu, 7/2/09, sr...@aol.com wrote: From: sr...@aol.com Subject:

[Histonet] Help With Hemo Fading

Hello everyone, ? We are having problems with short-term hematoxylin fading and loss of detail. The pathologist is freaking out! I've seen hemo fade over a long period of time but not in a matter of a few months. Slides from one year ago are really bad. ? I've been out of the business for a

[Histonet] help needed with Tissue-Tek DRS2000

Hi, I have recently acquired a Tissue-Tek DRS 2000 and I am wondering if there would be someone I could chat with about setup and operation of this stainer. If you would be willing to share your knowledge on this machine please let me know. My phone number is 360-966-7300. Thanks,

RE: [Histonet] help needed to find parts for tissue processor

Services, Inc. 404.291.3715 -Original Message- From: Leroy Brown [mailto:rhbro...@histocs.com] Sent: Wednesday, May 27, 2009 12:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] help needed to find parts for tissue processor Hi , I have two MVP-1 tissue processor that are

[Histonet] help needed to find parts for tissue processor

Hi , I have two MVP-1 tissue processor that are still in great shape except that both have lost the display screens. Other than that they are wonderful tissue processors.I am told by the folks that "know" this machine that I can swap out these screens from any working model from any

AW: [Histonet] help

uftrag von Dertien, Janet Gesendet: Sonntag, 26. April 2009 21:17 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] help Hello, I have a friend who is having trouble with ziehl-neesen stainin. Her sections appear red (with no blue). I have a feeling she may not be destaining adequately. Any s

Re: [Histonet] help

section is the end point of the destaining. René J.  --- On Sun, 4/26/09, Dertien, Janet wrote: From: Dertien, Janet Subject: [Histonet] help To: histonet@lists.utsouthwestern.edu Date: Sunday, April 26, 2009, 3:16 PM Hello, I have a friend who is having trouble with ziehl-neesen stainin. Her

[Histonet] help

Hello, I have a friend who is having trouble with ziehl-neesen stainin. Her sections appear red (with no blue). I have a feeling she may not be destaining adequately. Any suggestions? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu on behalf of histonet-requ...@lists

FW: [Histonet] help with muscle stains

lding, University of Glasgow, Glasgow, G12 8QQ. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: 16 March 2009 04:10 To: histonet Subject: [Histonet] help with muscle stains Would anyone li

[Histonet] help with muscle stains

Would anyone like to share their procedures for Cytochrome oxidase(COX), Succinic dehydrogenase (SDH) and NADH-TR with me? I have old, old ones but they are sketchy. Thanks. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville,

[Histonet] Help with McDowell and Trump's Fixative 4F:1G for EM samples

Hello!  Can anyone give me some info on McDowell and Trump's fixative?  We have a protocol that requests the formulation of 4F:1G of McDowell and Trump's fixative that we are going to be collecting electron microscopy samples in.  Does anyone know if this is a specific formulation that must be m

[Histonet] Help, Histotechnologist with grossing experience

Hi members of Histonet, I am having a hard time finding Histotechnologists in the Boston, MA area who have experience in Grossing ... could someone help me or guide me in the right direction? It looks like I can only find PA's who have this specific skill. I am recruiting around a Histotech, G

Re: [Histonet] Help with antigen unmaksing on frozen sections

u pictures if u want,. 2009-02-12 TF 发件人： Sarah Tarran 发送时间： 2009-02-11 12:19:58 收件人： histonet 抄送： 主题： [Histonet] Help with antigen unmaksing on frozen sections Hi everyone, I some help with antigen on frozen sections as I am having a lot of trouble getting antibodies to work. I

Re: [Histonet] Help with antigen unmaksing on frozen sections

Sarah, Try fixing your cut sections in 10%NBF for 20-30 minutes after deparaffinizing, then do the retrievals. SMA doesn't usually require any retrieval. TCR beta works well with pepsin for 20 mins. - this is less harsh than proK. I'm not familiar with the other abs you mention. Jo Mauger >>> "S

[Histonet] Help with antigen unmaksing on frozen sections

Hi everyone, I some help with antigen on frozen sections as I am having a lot of trouble getting antibodies to work. I am using a GFP mouse so can't formalin fix, parafin embed my samples. I tried frsh freezing but found that the GFP leaked so I couldn't use that either. So I currently fix my tiss

[Histonet] help with muscle histology

Fellow Histonetters...any help would be appreciated. I have a couple questions about getting good muscle samples. I have a project in the lab right now that is working with laser. The student is taking rat legs for this. What he does is cut a laser line into the large muscle of the rat lag at

Re: [Histonet] Help with protocols

If the CC1 standard 32 with amp, 42 degrees does not work then try using protease 1 4-6 minutes on it. On Fri, Nov 28, 2008 at 12:35 PM, Lena Spencer <[EMAIL PROTECTED]>wrote: > Hi All: > > I have been working up the PMS2 and MLH2 mismatched repair genes and I am > not satisfied with my results.

[Histonet] Help with protocols

Hi All: I have been working up the PMS2 and MLH2 mismatched repair genes and I am not satisfied with my results. I am using the Cell Marque antibodies and the Ventana XT using CC1, mild, standard and I have tried the antibodies with block and amplification, and antibody times from 32 min- 1 hour

[Histonet] help

The chicken came first--but she did not have a belly button... Ashley Troutman BS, HT(ASCP)QIHC Histopathology Laboratory Department of Pathology Vanderbilt University Medical Center Nashville, TN

[Histonet] help with Zeiss Apotome

I have inherited a new Zeiss Axiovert system with an Apotome. Our sales rep has helped me to learn the system but he says that Zeiss has no user manual for this microscope and attachments. Does anyone have a protocol for using these instruments, possibly a core facility with multiple users. Than

[Histonet] help-fibrotic tissue

I need to finally call on the experts.? I have tried all of the tricks up my sleeve to cut extremely fibrotic Cerivical tissue.? It is not calcified, just fibrotic...it is like cutting glass. Help. Roxanne ___ Histonet mailing list Histonet@lists.uts

[Histonet] help! buffer calculation

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