Messages by Thread
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How do I apply the genome scale reconstruction for E. coli in my experiments
Yoginee Budhkar
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Re: Any experiences with Geneamp 9700 cycler?
Michael Sullivan
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RNA isolation from Formaldehyde fixed samples
Pow Joshi
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Retroviral vector packaging compatibility
Ed Siefker
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protein estimation
Sudheer Sangeetham
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Molisch test gives green layer?
Gerchman
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Quantum Yield of Ethidium Bromide?
Ayesha Ihsan
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Sad CO2 Incubator...
Yvonne Couch
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RE: LiCl precipitation?
Hiranya Roychowdhury
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Running formaldehyde gels in 1x MOPS buffer?
Ron
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DNA extraction and PCR query
Sonal Patel
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protein
akhatr hussain
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96 well inserts?
Gerchman
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His tag CBP TAG
Imran KHAN
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DNA Purification
JEWELLE GLASGOW
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Affinity chromatography on NHS activated agarose with small peptides
WS
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FFPE tissue rehydration - which xylene?
Gerchman
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Does Taq accespt ATP as substrate?
WS
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phenol/CH3 extraction--no emulsion?
Ed Siefker
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protein extinction coefficient
Sudheer Sangeetham
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qPCR & NGS 2013 - EARLY BIRD registration peroid & ABSTRACT CALL - deadline 31st Jan 2013
Editor www.Gene-Quantification.info
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Incorporation efficiency of alpha-P32 dNTP in PCR
Vishal Chaudhari
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Re: Amplifying gene of interest from clinical isolate !!!
Cathal Garvey
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Gel Purifying tRNA
Rachel Schmidt
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Re: Calculating Protein Concentration
Dr Engelbert Buxbaum
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Homebrew Dark Reader amber screen?
WS
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Expression vector for GST-tagged TEV protease
Phelan, Paul J.
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before thinking about the contamination
dora
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Where to obtain a copy of RAPDFst?
ke
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Kanamycin Stability, & Mysterious Orange Colonies
Cathal Garvey
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siRNA Transfection Reagent for THP-1
Babak Memari
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Re: Methods Digest, Vol 90, Issue 2
sudheer sangeetham
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Removing RNAse from resuable tubes
Ed Siefker
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Ni-NTA column turns yellow colour
sudheer sangeetham
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qPCR NEWS - October 2012 - focus on RNA integrity
Editor www.Gene-Quantification.info
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Re: Recombinant protein with specific cleavage in fungi
Cathal Garvey
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Re: Recombinant protein with specific cleavage in fungi (toufic el arnaout)
Toufic Arnaout
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RE : RNA shipment (WS)
Perreault, Jonathan
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Antibodies against short peptide sequences (5mer)
WS
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Haunted Biologic DuoFlow system?
Phelan, Paul J.
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qPCR NEWS September 2012 - focus on CNA (circulating nucleic acids)
Editor www.Gene-Quantification.info
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chromatography columns
WS
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RNA shipment
Peter Ellis
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G418 selection
Mukherjee, Sumit
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Rheumatoid arthritis (RA) biomarker and drug discovery assays and protocols
kar . sanchayita
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Re: S35 Pulse Chase analysis of an endogenously expressed glycoprotein
Dr Engelbert Buxbaum
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Folic Acid solubility in bicarbonate solutions
Justin Washizu
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S35 Pulse Chase analysis of an endogenously expressed glycoprotein
Vladimir Gainullin
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qPCR NEWS - August 2012 - focus on single-cell qPCR
Editor www.Gene-Quantification.info
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new forum to buy and sell equipment
Mary
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Overgrown (3day) LB plates ok to use?
David Liu
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Southern Blot hybridization question
Vladimir Gainullin
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Krebs buffer cloudy
Gretha Van Oorschot
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Re: Methods Digest, Vol 86, Issue 8
sudheer sangeetham
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Hello...problem of detecting proteins with CBB staining
sudheer sangeetham
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qPCR NEWS - July 2012 - focus on microRNA
Editor www.Gene-Quantification.info
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pGEM-T contamination
Miguel Azevedo
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regarding pngase f treatment
divya teja
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regarding the PNGase F treatment
divya teja
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hair root RNA isolation
JAVIER RUIZ EDERRA
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PCR TAopt calculator
Laurent Boulanger
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pI shift be Phosphorylation?
Gerchman
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Best nuclease for removing DNA from bacterial lysates?
Phelan, Paul J.
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qPCR NEWS June 2012 - first announcement qPCR & NGS 2013
Editor www.Gene-Quantification.info
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removal of DMSO from plant extract
Dr Omar Hamarsheh
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Doubt regarding protein dialysis and r-protein purification????
sudheer sangeetham
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recommendation for Fermenter for Pichia Pastoris
ab
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Removal of Ethidium Bromide from Skin
shiwin k.k
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Amino acid free media required
Sonal Patel
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GATEWAY CLONING
Philip O'Brien
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cloning oligos
Ed Siefker
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RE: regarding volume to be maintained in 12ml ultracentrifuge tubes during ultracentrifugation (divya teja)
Nikola Wenta
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regarding volume to be maintained in 12ml ultracentrifuge tubes during ultracentrifugation
divya teja
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Re: DNA sequence alignment
Don Gilbert
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Regarding primary antibody binding in western blot
divya teja
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Some funny (?) comics
WS
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RE: Inoue 1990 original paper for SEM transformation
Jayakumar, R
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Make Concentrated Solutions of hydrophobic amino acids
Sonal Patel
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Re: Methods Digest, Vol 84, Issue 1
Nikola Loncar
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Membrane_protein_purification with_HIC
Theresa H
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Trypsin and chemotrypsin colorimetric assay
Gerchman
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Ammonium sulfate precipitation from solutions with high sugar content
WS
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Protein concentration with PEG
WS
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qPCR Newsletter - April 2012 with focus on RNA integrity
Editor www.Gene-Quantification.info
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oligonucleotide-polypetide conjugation
alexandra balliou
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A minimal medium for S. aureus RN4220
Morooj
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Digestion problems with NaeI
Edward Valera
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Error prone PCR with nucleotide analogs and high fidelity polymerase
David Liu
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Strain requirements for routine cloning in phagemid M13 vector? (eg. pSEX81)
David Liu
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Re: Methods Digest, Vol 82, Issue 6
sudheer sangeetham
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trouble with membrane protein expression
heraa rajandas
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Re: Methods Digest, Vol 82, Issue 4
sudheer sangeetham
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increasing or reducing pH of buffers when I am adding MQ water
sudheer sangeetham
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Tunel Assay
Shar Waldrop
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Microbiology training
CUC3
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Could anyone please suggest me nontreated multiwell plate for photocrosslinking...
sudheer sangeetham