Hi Charles
I have done this stain many times as an abbreviated version of Movats
Pentachrome.
You are correct in thinking the van gieson will affect the AB stain due to the
acid.
As in the Movat stain do AB first then place slides in alcoholic ammonia for 1
hour to convert AB to insoluble
Just curious, why not do a Movat’s Pentachome? It gives you everything you are
looking for and more.
Best regards,
Akemi Allison-Tacha, BS, Ht, HTL
akemiat3...@gmail.com
Sent from my iPhone
> On Mar 9, 2023, at 11:19 AM, Paula Keene Pierce via Histonet
> wrote:
>
> Hi,
> if they are
Hi,
if they are wanting to stain elastic, Alcian blue, and Van Gieson, I recommend
doing the Verhoff elastic stain first, then the Alcian blue, and then the Van
Gieson.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N
Blue Lake DriveNorman, OK 73069PH
I am working with a researcher and they saw an article where someone
performed an Alcian Blue and elastic van Gieson double stain. If anyone
has done this before can you provide your protocol. I am trying to figure
out the best way to perform this process to get the best staining results.
Here
has increased its prices for
many pigments and dyes by up to 15% worldwide.
biologicalstaincommission.org
John Kiernan
Secretary. Biological Stain Commission
= = =
From: Charles Riley via Histonet
Sent: October 9, 2020 10:14 AM
To: histonet@lists.utsouthwestern.edu
To: histonet@lists.utsouthwestern.edu
Subject: [External] [Histonet] Alcian Blue staining
Warning: This email originated from the Internet!
DO NOT CLICK links if the sender is unknown, and NEVER provide your password.
We are having an issue where the goblet cells in our control tissue are not
staining pink
We are having an issue where the goblet cells in our control tissue are not
staining pink even though the patient tissue is staining beautifully on the
same slide.
The stain is done manually. What can be some causes for the issues in staining
on the control section?
Hello Histo Peeps:
I have two new lab coats which have been stained with Alcian Blue and can’t get
the stains out. I have tried oxy clean incorporated with bleach, as well as
pre-soaking in 3% acetic acid, and American Mastertech’s stain remover to no
avail. Any suggestions would be greatly
August 2018 11:22:55
To: Hobbs, Carl
Cc: histonet
Subject: Re: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives?
Hello Carl,
Alcianblue is q quite interesting stain! Maybe this selection of a presentation
helps you to find a suitable method. I attach you a PDF-document to this email
From: Mac Donald, Jennifer
Sent: 23 August 2018 19:52:52
To: Hobbs, Carl
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives?
The hyaluronidase is digesting out hyaluronic acid, a component found in
connective
blue
pH 2.5 and negative for pH 1.0.
-Original Message-
From: Hobbs, Carl via Histonet
Sent: Thursday, August 23, 2018 11:32 AM
To: histonet
Subject: Re: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives?
Perhaps Alcian blue at pH3 ( all acidic mucins) on one slide
Perhaps Alcian blue at pH3 ( all acidic mucins) on one slide/section and Ab
pH1 ( highly sulphated mucins only) on a sequential slide?
Sure, instead use Alcian blue CEC method?
I will be interested to read other responses to your request.
Mucin-illy
carl
Carl Hobbs FIBMS
Histology and
Help? we have a student with one of the online schools and we're doing
specials like it is 1972. She has to do a Al Blue 2.5 w Hyaluronidase
digestion. We haven't done this since the development of clinical IHC two
dozen years ago -
Is there any other way to acheive this outcome on a colon
Dear Histonetters,
I recently replied to a question about staining extracellular ground
substance with alcian blue. Here is some more information.
With the alcian blue-PAS sequence it is necessary to have a good alcian blue
dye - the same as or functionally equivalent to the original alcian
What is the shelf life of prepared 1% Alcian blue in 3% Acetic Acid?
Diana
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Histonet@lists.utsouthwestern.edu
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Until the staining starts to weaken
René J.
From: Diana McCaig dmcc...@ckha.on.ca
To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu
Sent: Wednesday, February 27, 2013 2:55 PM
Subject: [Histonet] Alcian Blue
What is the shelf life of prepared 1% Alcian blue in 3% Acetic
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, February 27, 2013 11:57 AM
To: Diana McCaig; 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Alcian Blue
Until the staining starts to weaken
René J.
From: Diana
-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth
Chlipala
Sent: Wednesday, February 27, 2013 3:03 PM
To: Rene J Buesa; Diana McCaig; 'histonet@lists.utsouthwestern.edu'
Subject: RE: [Histonet] Alcian Blue
Why would you use a reagent until
-0500
Subject: [Histonet] Alcian Blue
Hi Everyone:
Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue
and some say 10 min?Our control works well at 10 minutes but today I had a
Dr. say that he expected a small amount of cells to stain and they didn't
.
Peggy A. Wenk, HTL(ASCP)
Beaumont Hospital
Royal Oak, MI 48073
Opinions expressed do not reflect upon Beaumont Hospital.
-Original Message-
From: Sheila Adey
Sent: Thursday, December 06, 2012 8:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Alcian Blue
Hi Everyone
: [Histonet] Alcian Blue
Hi Everyone:
Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue
and some say 10 min?Our control works well at 10 minutes but today I had a Dr.
say that he expected a small amount of cells to stain and they didn't in an
esophagus bx.So, now I'm
; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Alcian Blue
We've always used 30 minutes and it has never failed.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Adey
Sent: Thursday
procedure
calls for 30 min, unless you use a microwave oven version of the staining
protocol.
René J.
From: Sheila Adey sa...@hotmail.ca
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Thursday, December 6, 2012 8:58 PM
Subject: [Histonet] Alcian Blue
Hi Everyone
Hi Everyone:
Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue
and some say 10 min?Our control works well at 10 minutes but today I had a Dr.
say that he expected a small amount of cells to stain and they didn't in an
esophagus bx.So, now I'm wondering if b/c most of
allison, we do all of our alcian blue on the machine. we love it!!
anita dudley
providence hosp
mobile, ala
From: allison_sc...@hchd.tmc.edu
To: histonet@lists.utsouthwestern.edu
Date: Fri, 21 Oct 2011 14:00:44 +
Subject: [Histonet] Alcian blue on the Ventana/Cryoembedding Apparatus
Hello to all in histo land. For those of you that have the ventana special
stains machine, can the alcian blue 2.5 be done on the machine? I am not sure
if they have kits for this stain. Also is anyone using the cryoembedding
apparatus from pathology innovations? Thanks in advance.
Allison
: Freitag, 21. Oktober 2011 16:01
An: 'histonet@lists.utsouthwestern.edu'
Betreff: [Histonet] Alcian blue on the Ventana/Cryoembedding Apparatus
Hello to all in histo land. For those of you that have the ventana special
stains machine, can the alcian blue 2.5 be done on the machine? I am not
sure
Hello everybody,
I was wondering if anyone has a protocol for Alcian Blue-Safranin (for mast
cells).
I would greatly appreciate any help.
Thank you!
Leticia Figliuolo
e-mail: leticia.figliu...@roche.com
___
Histonet mailing list
We are having a problem with our alcian blue pH2.5 stain. It is taining the
collagen really dark and the mucin really light. My boss just handed me this
assignment today and told me to find out what is wrong with it, however I am
not very familiar with this stain. He suggested I remake all
hi every body
i have a confusing quistion reagrding Alcian blue pH 1 2.5 stains.
suppose you have 100 goblet cell react positive to pH 1 which mean all have
sulfated mucin. and 50 cell from other section react positive to pH 2.5 which
mean acidic type(sulfated or sialated). why these
hi every body
i have a confusing quistion reagrding Alcian blue pH 1 2.5 stains.
suppose you have 100 goblet cell react positive to pH 1 which mean all have
sulfated mucin. and 50 cell from other section react positive to pH 2.5 which
mean acidic type(sulfated or sialated). why these 50 cell
Does anyone out there do this stain? Alcian Blue-Acid mucosaccharides
with and without hyaluronidase? Where do you get the testicular
hyaluronidase? Thank you so much. This for a student.
Kathy Gorham H.T.
GRH National Recognition
Outstanding Rural Health Organization of 2009 awarded by NRHA
On 3/3/2011 1:47 PM, Kathy M. Gorham wrote:
Does anyone out there do this stain? Alcian Blue-Acid mucosaccharides
with and without hyaluronidase? Where do you get the testicular
hyaluronidase? Thank you so much. This for a student.
Kathy Gorham H.T.
You can get the enzyme from Sigma. Note
Our lab has a request for alcian blue/alizarin red staining on mouse kidney
teratomas. This will be done on FFPE slide mounted sections. In the past, we
have done skeletal staining using these stains which also involved all of the
clearing steps using potassium hydroxide.Has anyone done
/~cemrf
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas,
Nancy
Sent: Tuesday, April 20, 2010 12:49 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Alcian blue alizarin red double stain
Hi Everyone,
Lately, we have been having a problem with our Alcian Blue. It is fine
up to the washing step. Once it hits the water, the blue fades to
unacceptable. We have tried reducing the water rinse after the blue to
just a quick rinse in d-H20, and it is still poor. The vendor replaced
the
The pH of the stain may be too high, it should be 2.5-2.6 so check it
with a pH meter.
If the pH is fine you/your vendor may have a bad batch of dye since
under normal conditions the stain is difficult to wash out.
Make sure the dye is certified by the Biological Stain Commission. If
there is
] Alcian Blue 2.5
To: histonet@lists.utsouthwestern.edu
Hi Everyone,
Lately, we have been having a problem with our Alcian Blue. It
is fine
up to the washing step. Once it hits the water, the blue fades to
unacceptable. We have tried reducing the water rinse after the
blue to
just a quick
Hi Margaret
You perform the Alcian Blue first but as with Movatt's pentachrome you need to
treat the sections after AB with alcoholic ammonia for an hour to convert the
AB to Monastral Blue as the AB is soluable in picric acid and the Monastral
blue resistant. Let me know if you need a method
We want to try the double stain alcian blue Van gieson. Do you stain for the
alcian blue first and then the Van gieson? We do both stains but I am unsure
of how to put them together.
Margaret Perry
South Dakota State University
___
Histonet mailing
Alcian Blue first
- Original Message
From: Perry, Margaret margaret.pe...@sdstate.edu
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Fri, March 12, 2010 9:30:40 AM
Subject: [Histonet] alcian blue van gieson stain
We want to try the double stain alcian
fragile and horribly expensive. Replacing them is a big item in every lab's
budget.
John Kiernan
Anatomy, UWO
London, Canada
= = =
- Original Message -
From: Diana McCaig dmcc...@ckha.on.ca
Date: Sunday, December 6, 2009 13:49
Subject: [Histonet] Alcian Blue
To: histonet
In the past, when we made up 1% Alcian blue in 3% acetic acid (using
bottle distilled water), the pH was always 2.5. We did not have to
modify it to get the proper pH. Still using the same powder, we are
getting a pH of 2.1. I hate to adjust it because it seems to affect the
staining. Any
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