Re: [Histonet] (no subject)

[Tony Reilly via Histonet]

Hello Rhonda
I have extensive experience with the Brown Hopps stain.

I would advise against only staining for gram negative as both the gram 
positive stain and the gram negative stain will stain both gram positive and 
gram negative organisms.  The differentiation is only achieved by the 
destaining steps.

If you only used the gram negative steps you would still be staining both gram 
positive and gram negative organisms negatively.

If you you want more information let me know .

Regards
Tony Reilly
Histologist
Australia



Sent from my iPhone

> On 4 Jul 2023, at 5:30 am, Mac Donald, Jennifer via Histonet 
>  wrote:
> We have good success with the Twort.
> 
> -Original Message-
> From: Rhonda McCormick via Histonet 
> Sent: Monday, July 3, 2023 11:22 AM
> To: Histonet 
> Subject: [Histonet] (no subject)
> 
>  EXTERNAL SENDER - Exercise caution with requests, links, and attachments.
> 
> Hello, I'm wondering if anyone does the Brown-Hopps Gram stain for gram 
> negative bacteria. If so, would you mind to send your protocol please?
> Any recommendations for a gram negative (only) stain - specifically E. coli? 
> One of our residents is asking.
> Thank you!
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Re: [Histonet] (no subject)

[Mac Donald, Jennifer via Histonet]

We have good success with the Twort.

-Original Message-
From: Rhonda McCormick via Histonet 
Sent: Monday, July 3, 2023 11:22 AM
To: Histonet 
Subject: [Histonet] (no subject)

  EXTERNAL SENDER - Exercise caution with requests, links, and attachments.

Hello, I'm wondering if anyone does the Brown-Hopps Gram stain for gram 
negative bacteria. If so, would you mind to send your protocol please?
Any recommendations for a gram negative (only) stain - specifically E. coli? 
One of our residents is asking.
Thank you!
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Re: [Histonet] (no subject)

[Jay Lundgren via Histonet]

IIRC, yes, I seem to remember at AFIP our NCOIC cursing and reprogramming
Roberta every Friday, or for a three day weekend.

Roberta was the VIP's name.  That lovely science-fictiony tan and burnt
orange color scheme.  I wonder what happened to her?  Maybe you bought her?

They never would have let a lowly Airman touch her.

She would call you on the phone though, if her process was interrupted.  No
kidding, look around and you should find a land-line (of course) phone jack
on the back. Pretty cool stuff in 1987.

Sincerely,

Jay A. Lundgren, M.S., HTL (ASCP)

On Thu, Jun 22, 2023 at 8:42 AM Kate Bummer via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Help on how to program an artifact, aka, Miles Tissue Tek VIP 1000 tissue
> processor?
>
> Hello All,
>
> My company purchased the miles tissue tek vip 1000 tissue processor at an
> auction and I have a user manual that is not very useful in figuring out
> how to set up programs.  I was hoping to set up multiple programs but it
> looks like on the automatic it really only takes  1 program and you can set
> up start and end time but cannot set up multiple programs and would have to
> key in a new program every time.  That is ok and not my preferred situation
> as I've really only worked with a Leica ASP300S which of course is much
> newer and can store several programs.
>
> Hoping someone has worked on one of these and may have a user guide that
> is more useful than the user manual I currently have (from 1986 : /  )
>
> Please drop me a line if you have any info it would be much appreciated.
>
>
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Re: [Histonet] (no subject)

[Dr. Michael Gudo (Morphisto GmbH) via Histonet]

Dear Kate,

very interesting question.

The both substances you mention are a bit difficult in this respect. In a 
general crystal structure phosphortungstic acid has 44 water molecules and 
phosphormolybdic acid 28 molecules of crystal water. This would mean, that for 
a an exact 5% solution you would need around 72,62 g of phosphortungstic acids 
and 72,73 of phosphormolybdic acid on 1.000 ml water. The standard for a 5 % 
solution of a solid substance without crystal water would be 52,63 g per 1.000 
ml.

However, in this case, the substances do not exactly have this number of water 
molecules in the crystal structure because they loose water and they are 
hygroscopic. This means that they take more water molecules from the air and 
that they partly melt in the bottle, if leave it open to long. 
 „• x H20“ - does not mean, that the manufacturers don’t want to give this 
information, its because it cannot be known exactly.

For these substances histological practice is to ignore the crystal water and 
to use the 52,63 g substance for 1.000 ml of water to get a 5% solution (or to 
add 5 g substance to 95 g water).

This does not have an crucial effect, because the mordanting process for which 
these acids are used has to be controlled by microscopy anyway. So if you want 
to make it faster you can add more substance. The important point is only, to 
make it always the same way.

Kind regards
Michael




> Am 24.01.2020 um 16:51 schrieb Kate Davoli via Histonet 
> :
> 
> I'm all set to DYI my own phosphomolybdic/phosphotungstic acid solution for
> running a Masson Trichrome, but I see that the former reagent was
> purchased as "phosphomolybdic acid hydrate".
> 
> All the recipes I have seen call for just "phosphomolybdic acid" but that
> is not a reagent that appears to exist without the water molecules coming
> along for the ride, unless you want to invest in chromatography grade
> stuff, which I think histology folks probably don't routinely do.
> 
> The recipes all call for equal gram amounts of each of these crystals, so
> here's my question:
> 
> Do I calculate how much weight the water is taking up and add more
> phosphomolybdic acid crystals (to account for its tagalong water molecules)
> than called for in the recipe?  Or are these recipes already assuming that
> phosphomolybdic acid HYDRATE is the reagent you have on hand, and I should
> stick with equal amounts?
> 
> This question is somewhat complicated by the fact that the molecular
> formula on the bottle is listed as H3Mo12O40P.XH2O ... which I think means
> the manufacturer won't bet on exactly how many water molecules are involved.
> 
> Any advice appreciated!
> 
> Katherine Davoli, BA, HTL(ASCP)CM
> Supervisor & Lab Manager, Tissue Culture & Histology Core Module
> Ophthalmic and Visual Sciences Research Center
> Department of Ophthalmology, University of Pittsburgh
> Mail Stop Code: EEI010901
> 930 Eye & Ear Institute, 203 Lothrop Street
> Pittsburgh, PA 15213
> (412) 647-8256davol...@upmc.edu and kdav...@gmail.com
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MORPHISTO GmbH
PD Dr. phil. nat. Michael Gudo
Weismüllerstr. 45
60314 Frankfurt am Main
Telefon:069 / 400 3019 - 62
Telefax:069 / 400 3019 - 64

E-Mail: michael.g...@morphisto.de 
Internet: http://www.morphisto.de/ 

Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo

Registergericht: Amtsgericht Frankfurt
Registernummer: HRB 74954
Umsatzsteuer-Identifikationsnummer gemäß § 27 a Umsatzsteuergesetz: DE243397199

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Re: [Histonet] (no subject)

[May Wei via Histonet]

   Hi Rhonda,

   To get contact with the local histotech community is a good place to
   start by contacting local community colleges which offer the histotech
   certificate (see the list of the local
   schools [1]https://www.nsh.org/learn/histology-schools). They may even
   want you to teach one of their lab classes given your extensive
   experiences.

   May Wei



    Original Message 
   Subject: [Histonet] (no subject)
   From: Rhonda McCormick via Histonet
   <[2]histonet@lists.utsouthwestern.edu>
   Date: Wed, August 14, 2019 3:10 pm
   To: [3]histonet@lists.utsouthwestern.edu
   Help!
   I became HT certified in 2004 under the HS degree and lab experience
   qualification. I do have a Bachelorâs Degree in Education,, with only
   12 hours of science. I am looking to relocate to Texas and therefore am
   job hunting. The problem Iâm running into is that without an Associates
   Degree or Bachelors in a Science related field, I am not being
   considered for job opportunities even though I have 17 years of
   experience (and am a good histo tech and good employee with great
   references).
   Is anyone else running into this problem? Does the Histonet world
   recommend I go back to school - or just be patient, trusting a job will
   eventually come along?
   Thanks!
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References

   1. https://www.nsh.org/learn/histology-schools
   2. mailto:histonet@lists.utsouthwestern.edu
   3. mailto:histonet@lists.utsouthwestern.edu
   4. mailto:Histonet@lists.utsouthwestern.edu
   5. http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Re: [Histonet] (no subject)

[Morken, Timothy via Histonet]

Rhonda, that is unfortunate. It seems with the shortage of histotechs in most 
places that experience would be valued. Certainly you qualify since you were 
certified under the rules at the time. But institutions can set their own 
requirements as well which may be more strict. I wonder if your applications 
are being kicked out by automated programs that simply reject applications if 
they don't find certain terms.  Maybe the managers in the department never even 
see your application. I have had applicants in this situation and work with my 
HR department to have them clear such applicants so I can at least get them in 
the system for consideration even if they don't meet all requirements.


Maybe try to find out real people to contact at the institutions pathology 
department and deliver resumes directly to them rather than thru the online 
application system they may have. That way they can have their HR send your 
application through.


Tim Morken


From: Rhonda McCormick via Histonet 
Sent: Wednesday, August 14, 2019 3:10:02 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] (no subject)

Help!

I became HT certified in 2004 under the HS degree and lab experience 
qualification.  I do have a Bachelor’s Degree in Education,, with only 12 hours 
of science.  I am looking to relocate to Texas and therefore am job hunting.  
The problem I’m running into is that without an Associates Degree or Bachelors 
in a Science related field, I am not being considered for job opportunities 
even though I have 17 years of experience (and am a good histo tech and good 
employee with great references).
Is anyone else running into this problem? Does the Histonet world recommend I 
go back to school - or just be patient, trusting a job will eventually come 
along?
Thanks!
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Re: [Histonet] (no subject)

[MARY ANN via Histonet]

Cindy I can message you about it


Sent from Xfinity Connect Application

-Original Message-

From: histonet@lists.utsouthwestern.edu
To: histonet@lists.utsouthwestern.edu
Sent: 2018-10-02 8:31:45 AM 
Subject: [Histonet] (no subject)


Can anyone out in histo land give me feedback on the IHC instrument by StatLab 
Quantum HDX and how it compares to other automated platforms.  Thanks in 
advance!


Cindy Bird
Anatomical Medical Laboratories, Inc.
1600 Scripture Street
Denton, TX  76201
940-384-6210
940-384-6000
Fax 940-565-9588

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Re: [Histonet] (no subject)

[Bryan Llewellyn via Histonet]

All of the Picro-Mallory variants are trichrome stains. An explanation 
of how they work is here:


http://stainsfile.info/StainsFile/theory/tri_gen.htm
http://stainsfile.info/StainsFile/stain/fibrin/fibrin.htm

Follow the links as well for added information.

Bryan Llewellyn



Пешков Максим via Histonet wrote:


Dear Histonetters!
I need in your proffessional help.
Can you explain for me a the chemical mechanism of Picro-Mallory V stain by 
chemcial language?
I will appreciate references about this issue except original article of 
Lendrum A.C. et al (1962)  
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC480427/pdf/jclinpath00070-0009.pdf
Before asking I was read some histotechnological books, but can not find it. They 
are: Bancroft (from 5 to 8th ed), F. Carson A Self-instructional text 3 
ed, C.F.A. Culling (3rd ed), AFIP manuals (3-4 ed), Woods and Ellis (Histology lab: 
A complete reference), Lilli RD, 1962, Romeis 2014 (18 auflage) and some others 
book.
-- Russia,
Taganrog,
Maxim Peshkov.
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Re: [Histonet] (no subject)

[Blazek, Linda via Histonet]

Try the BioCare antibody.  It's excellent.

-Original Message-
From: Nirmala Srishan via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, December 01, 2016 1:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Since, Cell Marque H. Pylori is not available,  can some one give a comparable 
antibody to order?  Thanks in advance


Nirmala Srishan








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Re: [Histonet] (no subject)

[Duddey, Aimee via Histonet]

We ran into this situation with TJC.  We had a very limited check off sheet.  
In effort to standardize processes across the laboratory we adopted a program 
very similar to the NY facility whose program is referenced in the TJC leading 
practice library.  It is very rigorous and time consuming but serves its 
purpose well.  Also, CAP has a competency assessment program specifically for 
histology that is a subscription based purchase regardless if you are 
accredited by them.

Aimee

-Original Message-
From: Nirmala Srishan via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, September 01, 2016 1:49 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Hi,

What is everyone doing about the Histology Staff competencies.  I know CLIA has 
several criteria for the med-tech competencies.  Since the Histotechs do not 
report out results, how is everyone implementing this process.  Any information 
is greatly appreciated.


Nirmala 







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patient care, clinical performance and workplace excellence.
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Re: [Histonet] (no subject)

[Morken, Timothy via Histonet]

Nirmala, Histotechs are not required to have competency assessment under CLIA 
regulations because, as you note, they do not interpret any tests or report any 
results. However, if they do grossing then they do require high complexity 
competency assessment.

Having said that, if there is some concern in an inspection, any deemed 
accreditation agency (CLIA, CAP or JC) inspector, or state inspector,  can ask 
how you determine competency of any lab staff member to do any task. It is up 
to the Laboratory Director to determine how that is done, and how often. An 
institution may do it differently than the standard CLIA regulations outline, 
less often and less rigorous, but will need to convince an inspector that it is 
acceptable. The easiest way is to be sure there will not be any problems is to 
follow the competency assessment criteria for medium and high complexity - that 
is accepted and familiar to the inspectors so they should have no problem 
accepting it for other staff. 

Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center




-Original Message-
From: Nirmala Srishan via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, September 01, 2016 10:49 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Hi,

What is everyone doing about the Histology Staff competencies.  I know CLIA has 
several criteria for the med-tech competencies.  Since the Histotechs do not 
report out results, how is everyone implementing this process.  Any information 
is greatly appreciated.


Nirmala 







Holy Name Medical Center is ranked among the top hospitals in the nation for 
patient care, clinical performance and workplace excellence.
Click here to learn more.

 Warning: The information contained in this message is privileged and 
CONFIDENTIAL and is intended only for the use of the addressee above. If you 
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copying, distribution, or taking of any action in reliance on the content of 
this message is strictly prohibited. If you have received this communication in 
error, please notify the sender by replying to this message, and then delete it 
from your system.




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RE: [Histonet] (no subject)

[Roy, Lisa]

How long do you bake slides for before staining, at what temperature?  Does 
your stainer use agitation?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carolyn Nelson
Sent: Thursday, April 30, 2015 11:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Hi, I was hoping someone can help me with tissue falling off the slides. I have 
tried regular slides with and without adhesive in the water bath. Charged 
slides with and without adhesive in the water bath. I have not changed the type 
of slides I’m using. All the chemicals are fresh in the processor and the stain 
line, as well as the paraffin in the processor. It is the worst on needle bx ( 
prostate and breast ). I am SO frustrated, any help would be greatly 
appreciated! 


Carolyn






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Re: [Histonet] (no subject)

[Shruti Shah]

Hi does any one doing mice tibia histology, we use to fix in formalin for 24 
hours and transfer in 2and half weeks in 0.5M EDTA three change and process for 
24 hours long protocol in automatic processor.
But I am facing problem with bone marrow shrinkage. If any one have idea for 
decalcification timing and solution can resolved this problem and keep bone 
marrow intact with bone.
Thank you in advance.

Regards,
Shruti

Sent from my iPhone

 On 21 Apr 2015, at 8:51 am, Garrey Faller garr...@gmail.com wrote:

 Here is the CAP checklist requirement:
 ANP.21450
 All  histochemical stains are of adequate quality, and daily controls are
 demonstrated on each day of use for the tissue components or organism for
 which they were designed.

 Ray...you should call the CAP and ask for guidance on this.
 My interpretation of this requirement is that it should be OK to use a
 fungus from an orange peel. An orange peel fungus should have the same
 staining characteristics as a candida or aspergillus etc.  Similarly a
 bacteria is a bacteria. If you can produce a control that has both gram
 positives and negatives, it should be OK. But, don't quote me on this.

 Call the CAP for a definitive answer. I am interested in their response.
 Garrey

 On Sun, Apr 19, 2015 at 9:06 PM, koelli...@comcast.net wrote:

 I asked about this in a different vein months ago.  Has anyone shown a
 strawberry or ground meat or slim jim or orange peel as a bacteria/fungus
 control used for diagnostics to an inspector inspecting the lab and was
 there any comment from the inspector either positive or negative. Never
 heard back anything.
 Ray, Lake Forest Park, WA

 - Original Message -

 From: tjfinney2...@gmail.com
 To: histonet@lists.utsouthwestern.edu
 Sent: Sunday, April 19, 2015 5:24:53 PM
 Subject: [Histonet] (no subject)

 GMS controls
 From my understanding we can't use non human controls on patients. I
 could be wrong, but you may want to look into it.

 Happy Connecting.  Sent from my Sprint Phone.

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Re: [Histonet] (no subject)

[koellingr]

I asked about this in a different vein months ago.  Has anyone shown a 
strawberry or ground meat or slim jim or orange peel as a bacteria/fungus 
control used for diagnostics to an inspector inspecting the lab and was there 
any comment from the inspector either positive or negative. Never heard back 
anything. 
Ray, Lake Forest Park, WA 

- Original Message -

From: tjfinney2...@gmail.com 
To: histonet@lists.utsouthwestern.edu 
Sent: Sunday, April 19, 2015 5:24:53 PM 
Subject: [Histonet] (no subject) 

GMS controls 
From my understanding we can't use non human controls on patients. I could be 
wrong, but you may want to look into it. 

Happy Connecting.  Sent from my Sprint Phone. 

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RE: [Histonet] (no subject)

[Kelley, Amanda]

Hello my friend, I hope you are well.  Sorry Cherie, but the CAP checklist 
requires that you use a Lab grade microwave.   I would recommend that you order 
the BP-110 laboratory grade microwave,  it must be vented.  Since you're in 
Missouri, I would recommend contacting Lab Storage systems in St. Peter's Mo. 
1-800-345-4167

Amanda Kelley HTL
Dermatopathology Center at Washington University
Cortex One Suite 212
Phone: 314-362-5759
Fax: 314-362-5701
akel...@path.wustl.edu




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chapman, Cherie 
J.
Sent: Thursday, February 19, 2015 2:19 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Does anyone know for CAP accreditation if you can still use a regular microwave 
in a lab?

Thanks,

Cherie

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Re: [Histonet] (no subject)

[Rene J Buesa]

Yes, you have to. The origin of the section (MOHS or other) is not the criteria 
to use controls, but the process is.René J.  

 On Tuesday, December 2, 2014 4:23 PM, Chapman, Cherie J. 
chapm...@health.missouri.edu wrote:
   

 Does anyone know if you have to use controls for IHC on MOHS frozen section 
procedures to satisfy CAP requirements?

Thank you,

Cherie Chapman, BS, HT, HTL (ASCP)
Associate Director of Dermatopathology Laboratory
University of Missouri Department of Dermatology
University Physicians Medical Building
Phone: (573) 884-0123
Fax: (573) 884-0834

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RE: [Histonet] (no subject)

[Patsy Ruegg]

I would think you would have to validate IHC for MOHS the same as FFPE, once 
the validation process is done maybe controls would not be necessary.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net


 From: chapm...@health.missouri.edu
 To: histonet@lists.utsouthwestern.edu
 Date: Tue, 2 Dec 2014 21:22:38 +
 Subject: [Histonet] (no subject)
 
 Does anyone know if you have to use controls for IHC on MOHS frozen section 
 procedures to satisfy CAP requirements?
 
 Thank you,
 
 Cherie Chapman, BS, HT, HTL (ASCP)
 Associate Director of Dermatopathology Laboratory
 University of Missouri Department of Dermatology
 University Physicians Medical Building
 Phone: (573) 884-0123
 Fax: (573) 884-0834
 
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RE: [Histonet] (no subject)

[WILLIAM DESALVO]

University of Michigan MLabs

William DeSalvo, BS HTL(ASCP)
 
 From: ttro...@petersonlab.com
 To: histonet@lists.utsouthwestern.edu
 Date: Wed, 10 Sep 2014 12:22:55 -0500
 Subject: [Histonet] (no subject)
 
 I was wondering what facility labs are sending nerve and muscle biopsies to.
 
 
  
 
 Thanks,
 
 Travis Troyer
 
 Peterson Laboratory Services
 
 Manhattan, KS
 
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Re: [Histonet] (no subject)

[Karen]

You could send them to Beaumont Health System. 

Sent from my iPhone

 On Sep 10, 2014, at 1:31 PM, WILLIAM DESALVO wdesalvo@outlook.com wrote:
 
 University of Michigan MLabs
 
 William DeSalvo, BS HTL(ASCP)
 
 From: ttro...@petersonlab.com
 To: histonet@lists.utsouthwestern.edu
 Date: Wed, 10 Sep 2014 12:22:55 -0500
 Subject: [Histonet] (no subject)
 
 I was wondering what facility labs are sending nerve and muscle biopsies to.
 
 
 
 
 Thanks,
 
 Travis Troyer
 
 Peterson Laboratory Services
 
 Manhattan, KS
 
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RE: [Histonet] (no subject)

[Michael LaFriniere]

My favorite coverslipper at this time  is Leica due to needing glass cover 
slipping for imaging.

Michael
Michael R. LaFriniere, HT (ASCP) 
Executive Director
 

Capital Choice Pathology Laboratory
12041 Bournefield Way, Suite A . Silver Spring, MD 20904  
P: 240.471.3427 . F: 240.471.3401 . Cell 410-940-8844
michael.lafrini...@ccplab.com
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Saturday, July 26, 2014 11:32 AM
To: Aneesh Dhiman; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] (no subject)

Your request is quite odd. Are you looking for 5 guys to recommend or are you 
looking for a good coverslipper?
This is not an issue of obtaining 5 guys with opinions; what if the 5 guys 
advise you to buy the worst coverslipper?
I think you need to know the quality of the instrument first.
You should contact manufacturers and read specifications. Later you may select 
2 and submit those 2 to find out which of the two, according with the histonet 
guys is preferable.
I prefer Sakura.
There you have it: the opinion of one guy
René J. 


On Friday, July 25, 2014 4:21 PM, Aneesh Dhiman aneeshdhi...@gmail.com wrote:
  


Hi guys,  I need to settle on a coverslip machine, I wonder which is most
reliable need 5 peoples at minimum or 10 opinions for the recommendations
please
Aneesh Dhiman
University of Alberta hospital
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RE: [Histonet] (no subject)

[Sanders, Jeanine (CDC/OID/NCEZID)]

not odd at all.does it matter if it is a glass coverslipper or film? We 
have a Sakura glass coverslipper and a Leica glass coverslipper. Both have been 
great. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Saturday, July 26, 2014 11:32 AM
To: Aneesh Dhiman; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] (no subject)

Your request is quite odd. Are you looking for 5 guys to recommend or are you 
looking for a good coverslipper?
This is not an issue of obtaining 5 guys with opinions; what if the 5 guys 
advise you to buy the worst coverslipper?
I think you need to know the quality of the instrument first.
You should contact manufacturers and read specifications. Later you may select 
2 and submit those 2 to find out which of the two, according with the histonet 
guys is preferable.
I prefer Sakura.
There you have it: the opinion of one guy
René J. 


On Friday, July 25, 2014 4:21 PM, Aneesh Dhiman aneeshdhi...@gmail.com wrote:
  


Hi guys,  I need to settle on a coverslip machine, I wonder which is most 
reliable need 5 peoples at minimum or 10 opinions for the recommendations 
please Aneesh Dhiman University of Alberta hospital 
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RE: [Histonet] (no subject)

[Cooper, Brian]

I agree. The Sakura tape coverslippers are work horses and will not disappoint. 
Stick with Sakura's brand of tape as well. There are other brands of tape 
available, but we found them to be of terrible quality.

Brian Cooper
CHLA Histology Supervisor

-Original Message-
From: Rene J Buesa [rjbu...@yahoo.com]
Received: Saturday, 26 Jul 2014, 8:38AM
To: Aneesh Dhiman [aneeshdhi...@gmail.com]; histonet@lists.utsouthwestern.edu 
[histonet@lists.utsouthwestern.edu]
Subject: Re: [Histonet] (no subject)

Your request is quite odd. Are you looking for 5 guys to recommend or are you 
looking for a good coverslipper?
This is not an issue of obtaining 5 guys with opinions; what if the 5 guys 
advise you to buy the worst coverslipper?
I think you need to know the quality of the instrument first.
You should contact manufacturers and read specifications. Later you may select 
2 and submit those 2 to find out which of the two, according with the histonet 
guys is preferable.
I prefer Sakura.
There you have it: the opinion of one guy
René J.


On Friday, July 25, 2014 4:21 PM, Aneesh Dhiman aneeshdhi...@gmail.com wrote:



Hi guys,  I need to settle on a coverslip machine, I wonder which is most
reliable need 5 peoples at minimum or 10 opinions for the recommendations
please
Aneesh Dhiman
University of Alberta hospital
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RE: [Histonet] (no subject)

[Weems, Joyce K.]

Hello,

You will need to do that yourself at 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Best wishes!

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
awill.imdpath...@yahoo.com
Sent: Thursday, June 12, 2014 1:55 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Please remove me

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RE: [Histonet] (no subject)

[Anne Murvosh]

I just got back from the Mohs conference.  The Biocare people have the stain 
and told me they would come out to help work it up. Anne

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bethany
Sent: Thursday, May 29, 2014 1:23 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

The doctor I work for wants to use Mart 1 staining for Mohs on melanoma cases.
Any information on where to start? What equipment do we need? Where do we 
acquire the stains/antibodies, etc?
thx
Bethany
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RE: [Histonet] (no subject)

[joelle weaver]

What I did recently for two new processors, conventional type-
I  did parallel trial slides of multiple tissue types ( same types as patients) 
 for fixation, morphology , processing artifacts for 9 programs.
I grossed them in and recorded fixation times, type, thickness,  overall 
dimensions. I ran on the test programs. Then I embedded and sectioned and 
evaluated the  results by microscopic review by techs  then the medical 
director of  the H  E stained sections for each program and tissue type. 
Looking at any autolysis, nuclear detail, poor dehydration, other processing 
problems in each set. 
 
Then I just made a simple evaluation sheet for any tissue processing related 
issues, with a number rating/scale for the results. Retained records of the 
validation runs and the stained sections used for validation. Defined 
acceptable tissue types  and dimensions for the processing programs in the SOP, 
 and then I just created back up/recovery procedure and reprocessing procedure 
and ran through those for comparison. When completed, I just compiled into 
validation summary report. 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
 From: lmurp...@aultman.com
 To: Histonet@lists.utsouthwestern.edu
 Date: Thu, 3 Apr 2014 15:26:17 +
 CC: 
 Subject: [Histonet] (no subject)
 
 How is everyone validating the tissue processor for new CAP ANP.23045 
 question on function and verification of equipment?
 
 
 
 LeAnn Murphy
 
 Aultman Hospital
 
 Canton, Ohio
 
 
 
 
 
 
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Re: [Histonet] (no subject)

[Cristi Stephenson]

I second Toni's suggestion.  Definitely sounds like the paraffin is still
present on the lower sections of the slide.

On Wed, Dec 4, 2013 at 10:17 AM, Rathborne, Toni 
trathbo...@somerset-healthcare.com wrote:

 Could it be the heating/deparaffinization process? If the upper sections
 are staining more evenly, then maybe they are free from residual paraffin.
 Try extending the time in the ovens and/or xylene.

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chapman, Cherie J.
 Sent: Wednesday, December 04, 2013 1:12 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] (no subject)

 Hello all,

 I am looking for suggestions on issues with our HE stain.

 I supervised a Veterinary Diagnostic lab for over 27 years and produced
 top quality sections, HE's, special stains and IHC  on a variety of
 different species in our lab.
  I am currently working in a  Dermatopathology Lab and I am  finding
 inconsistent staining with our HE's. Working with just skin is a challenge
 all on its own.  We have made changes to our staining protocol and just not
 happy with the end product.
 What we are observing is inconsistent  staining on levels on the same
 slide.  The top section seems to stain more evenly than the middle and
 bottom sections.  I can actually see three different shades of color.   The
 specimens are ribboned sections so I know it is not from thick and thin
 sections.   We have looked at our processing times, microwave vs. oven
 times, staining reagents, different brands of hematoxylin and eosin,
 adjustments on staining times, tap water compared to distilled water.

 Our main processor is the Thermo Scientific STP-420 and our back up is the
  Sakura VIP V processor.I have been working with Thermo technical
 support thinking it might be a processing issue.  We have a Leica ST5020
 Multstainer/CV5030 Robotic Cover slipper we have made several changes that
 the technical teams has suggested to the reagents and staining time.  It's
 still not the quality that we are looking for.
 I have had culligan techs out several times to see if it could be
 something with  the water.

 We can run 100 slides the same day, same reagents and protocol and the HE
 color is so inconsistent.
  I would appreciate any suggestions in this matter.


 Cherie Chapman, BS, HT, HTL (ASCP)
 Associate Director of Dermatopathology Laboratory University of Missouri
 Department of Dermatology University Physicians Medical Building
 Phone: (573) 884-0123
 Fax: (573) 884-0834

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RE: [Histonet] (no subject)

[Truscott, Tom]

Hi Cherie, Since the top section is consistently better, then the possibilities 
lie in what happens after they are on the slide. As Toni recommends slides that 
are heated or deparaffinized vertically may have left over paraffin on the 
lower regions.Maybe you could also try depar with them up on their long edges. 
Staining slides vertically might also give this appearance if reagent times and 
rinsing are too short. Staining slides flat would point to slides not level or 
reagents not evenly dispersed on the slide. I would not rule out thick-thin on 
ribbons but that is probably not the case since the good section is always at 
the top. Good luck, Tom T

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Wednesday, December 04, 2013 10:17 AM
To: 'Chapman, Cherie J.'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject)

Could it be the heating/deparaffinization process? If the upper sections are 
staining more evenly, then maybe they are free from residual paraffin. Try 
extending the time in the ovens and/or xylene.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chapman, Cherie 
J.
Sent: Wednesday, December 04, 2013 1:12 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Hello all,

I am looking for suggestions on issues with our HE stain.

I supervised a Veterinary Diagnostic lab for over 27 years and produced top 
quality sections, HE's, special stains and IHC  on a variety of different 
species in our lab.
 I am currently working in a  Dermatopathology Lab and I am  finding 
inconsistent staining with our HE's. Working with just skin is a challenge all 
on its own.  We have made changes to our staining protocol and just not happy 
with the end product.
What we are observing is inconsistent  staining on levels on the same slide.  
The top section seems to stain more evenly than the middle and bottom sections. 
 I can actually see three different shades of color.   The specimens are 
ribboned sections so I know it is not from thick and thin sections.   We have 
looked at our processing times, microwave vs. oven times, staining reagents, 
different brands of hematoxylin and eosin, adjustments on staining times, tap 
water compared to distilled water.

Our main processor is the Thermo Scientific STP-420 and our back up is the  
Sakura VIP V processor.I have been working with Thermo technical support 
thinking it might be a processing issue.  We have a Leica ST5020 
Multstainer/CV5030 Robotic Cover slipper we have made several changes that the 
technical teams has suggested to the reagents and staining time.  It's still 
not the quality that we are looking for.
I have had culligan techs out several times to see if it could be something 
with  the water.

We can run 100 slides the same day, same reagents and protocol and the HE 
color is so inconsistent.
 I would appreciate any suggestions in this matter.


Cherie Chapman, BS, HT, HTL (ASCP)
Associate Director of Dermatopathology Laboratory University of Missouri 
Department of Dermatology University Physicians Medical Building
Phone: (573) 884-0123
Fax: (573) 884-0834

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RE: [Histonet] (no subject)

[Rathborne, Toni]

I wouldn't be too quick to blame the blades. Have you changed paraffin 
brands/temperatures or changed any of your processing schedules? Is the 
fixative/fixation process the same? Is the collecting location doing anything 
different/have they had a change in staff? 
Also, what type of blades are you using? It would be easier for people to 
respond if they had this information.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Leann M. Murphy
Sent: Thursday, November 14, 2013 11:04 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

I was just wondering if anyone was having difficulty cutting biopsies?  We have 
been using the same blades for years and now it is so difficult to get a 
ribbon.  There are three other area hospitals having the same problem. I have 
been trying samples from various vendors and the problem is still the same.  
Maybe the company that makes these microtome blades have cut costs and is now 
using a lower grade of metal for the blade.  I don't  know what it is, but it 
is driving everyone crazy.  Also, we are spending more money on blades because 
they do not last as long and of course this does not make our Manager happy.  I 
am just very frustrated.  Any suggestions?

LeAnn Murph, HT (ASCP)
Aultman Hosptial
Canton, Ohio
Technical Specialist
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RE: [Histonet] (no subject)

[CHRISTIE GOWAN]

Hi Clay,

Most hospitals have a tissue committee that makes decisions about surgical 
specimens. These decisions become hospital policy. Most hospitals state that 
once a specimen is removed it essentially becomes the property of the hospital. 
Release of specimens is usually after it has been accessioned and grossed in 
for pathology reveiw. There is usually a form for that type of release. The 
hospital would be at risk if they let the specimen leave without following 
hospital policy. I am not saying it is right or wrong but I can see the logic 
in it. Once the specimen has been processed through the hospitals pathology 
department then blocks and slides can be requested for 2nd opinions. Good luck 
in your search for answers.

Christie Gowan

 

To: histonet@lists.utsouthwestern.edu
Date: Thu, 12 Sep 2013 12:38:10 +
From: claymi...@hotmail.com
Subject: [Histonet] (no subject)

 
 
 
I need a little help on a Patient’s rights question.
 
 
It is my understanding that when a patient has a procedure, the patient has the 
right to request that those specimens be examined by a laboratory of their 
choosing.  i.e. EGD, colonoscopy, etc
 
 
I am in Arkansas.  
 
 
My father had a procedure yesterday at a local hospital.  I manage a pathology 
laboratory that specializes in the type of tissue that the procedure procured.  
When it was requested that the tissue be sent to my laboratory, the hospital 
staff refused to fulfill the request.  We asked multiple times for a release 
form so my father could take his tissue with him.  The administration employee 
we spoke to said there was no such thing and that patients were not allowed to 
take their specimens. One employee going so far as stating that if we wanted 
the tissue sent somewhere aside from their contracted laboratory, that the 
procedure would be canceled and my father would have to go somewhere else.   My 
father, not wanting to cause a fuss, let the issue go.   
 
 
Questions: 
 
Is it legal for a hospital to require that tissue specimens be sent to a lab 
they are contracted with? 
 
 
Are there any other actions we could have taken to make our requests be honored?
 
 
 
This is not meant to insult the hospital, but to give an explanation of the 
situation for context in answering the questions.

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RE: [Histonet] (no subject)

[Weems, Joyce K.]

Yes, regulations are set up that must be followed.  What I did in this 
situation, was request the slides afterward for review by my doctors.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph’s 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of clay milks
Sent: Thursday, September 12, 2013 8:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)




I need a little help on a Patient’s rights question.


It is my understanding that when a patient has a procedure, the patient has the 
right to request that those specimens be examined by a laboratory of their 
choosing.  i.e. EGD, colonoscopy, etc


I am in Arkansas.


My father had a procedure yesterday at a local hospital.  I manage a pathology 
laboratory that specializes in the type of tissue that the procedure procured.  
When it was requested that the tissue be sent to my laboratory, the hospital 
staff refused to fulfill the request.  We asked multiple times for a release 
form so my father could take his tissue with him.  The administration employee 
we spoke to said there was no such thing and that patients were not allowed to 
take their specimens. One employee going so far as stating that if we wanted 
the tissue sent somewhere aside from their contracted laboratory, that the 
procedure would be canceled and my father would have to go somewhere else.   My 
father, not wanting to cause a fuss, let the issue go.


Questions:

Is it legal for a hospital to require that tissue specimens be sent to a lab 
they are contracted with?


Are there any other actions we could have taken to make our requests be honored?



This is not meant to insult the hospital, but to give an explanation of the 
situation for context in answering the questions.



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Re: RE: [Histonet] (no subject)

[E. Wayne Johnson 朱稳森]

We work in the veterinary medical field and 98% of our work is with 
swine diseases
including some feed additive safety evaluations.  We provide pictures of 
all the lesions
that we see as part of the report we provide to farms.  It definitely 
takes more of our
time than it would to give a pathomorphologic description in English and 
Chinese
but we believe in transparency even though it might expose us somehow, 
realizing

that some things in pathology are art.

We are providing service and we think the best way is to be as transparent
as possible in everything that we do.

Stonewalling and secrecy generally breed contempt.
We try to be at our best even when we dont feel like it.

It is true that our subjects have economic value only and they are 
expendable

and the pigs are unlikely to see us in court.  But I think that even in
situations where there are emotions as with pets, and where there are
real human concerns about quality of care for human patients, transparency
and participation of all those concerned in the diagnositic and
evaluative process generally creates good will and cooperation and
good outcomes.


On 3:59, CHRISTIE GOWAN wrote:

Hi Clay,

Most hospitals have a tissue committee that makes decisions about surgical 
specimens. These decisions become hospital policy. Most hospitals state that 
once a specimen is removed it essentially becomes the property of the hospital. 
Release of specimens is usually after it has been accessioned and grossed in 
for pathology reveiw. There is usually a form for that type of release. The 
hospital would be at risk if they let the specimen leave without following 
hospital policy. I am not saying it is right or wrong but I can see the logic 
in it. Once the specimen has been processed through the hospitals pathology 
department then blocks and slides can be requested for 2nd opinions. Good luck 
in your search for answers.

Christie Gowan



To: histonet@lists.utsouthwestern.edu
Date: Thu, 12 Sep 2013 12:38:10 +
From: claymi...@hotmail.com
Subject: [Histonet] (no subject)




I need a little help on a Patient’s rights question.


It is my understanding that when a patient has a procedure, the patient has the 
right to request that those specimens be examined by a laboratory of their 
choosing.  i.e. EGD, colonoscopy, etc


I am in Arkansas.


My father had a procedure yesterday at a local hospital.  I manage a pathology 
laboratory that specializes in the type of tissue that the procedure procured.  
When it was requested that the tissue be sent to my laboratory, the hospital 
staff refused to fulfill the request.  We asked multiple times for a release 
form so my father could take his tissue with him.  The administration employee 
we spoke to said there was no such thing and that patients were not allowed to 
take their specimens. One employee going so far as stating that if we wanted 
the tissue sent somewhere aside from their contracted laboratory, that the 
procedure would be canceled and my father would have to go somewhere else.   My 
father, not wanting to cause a fuss, let the issue go.


Questions:

Is it legal for a hospital to require that tissue specimens be sent to a lab 
they are contracted with?


Are there any other actions we could have taken to make our requests be honored?



This is not meant to insult the hospital, but to give an explanation of the 
situation for context in answering the questions.

___ Histonet mailing list 
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RE: [Histonet] (no subject)

[Cartun, Richard]

Hospital By-Laws established by the Medical Staff and Administration may 
prevent tissue obtained within the hospital from going outside to another 
laboratory.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax
richard.car...@hhchealth.org


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of clay milks 
[claymi...@hotmail.com]
Sent: Thursday, September 12, 2013 8:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

I need a little help on a Patient’s rights question.


It is my understanding that when a patient has a procedure, the patient has the 
right to request that those specimens be examined by a laboratory of their 
choosing.  i.e. EGD, colonoscopy, etc


I am in Arkansas.


My father had a procedure yesterday at a local hospital.  I manage a pathology 
laboratory that specializes in the type of tissue that the procedure procured.  
When it was requested that the tissue be sent to my laboratory, the hospital 
staff refused to fulfill the request.  We asked multiple times for a release 
form so my father could take his tissue with him.  The administration employee 
we spoke to said there was no such thing and that patients were not allowed to 
take their specimens. One employee going so far as stating that if we wanted 
the tissue sent somewhere aside from their contracted laboratory, that the 
procedure would be canceled and my father would have to go somewhere else.   My 
father, not wanting to cause a fuss, let the issue go.


Questions:

Is it legal for a hospital to require that tissue specimens be sent to a lab 
they are contracted with?


Are there any other actions we could have taken to make our requests be honored?



This is not meant to insult the hospital, but to give an explanation of the 
situation for context in answering the questions.

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

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RE: [Histonet] (no subject)

[Manfre, Philip]

It's a bad question.  Generally the best temperature is probably between 55 
and 65, depending on the paraffin.  This would fall between the two latter 
answers.  Hmm  What to do?

Phil.

Philip Manfre, B.A., HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP45-251
PO Box 4
West Point, PA 19486

215-652-9750
215-993-0383 (fax)
philip_man...@merck.com




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Erin
Sent: Monday, September 09, 2013 9:28 AM
To: histonet
Cc: Naujokas, Agne; Meier, Melissa
Subject: [Histonet] (no subject)

Good morning all!

One of our fellows emailed me a question that she came across while studying 
for her boards:



I'm studying for my board exam and came across questions re: paraffin 
embedding.
It reads: best temperature for paraffin embedding is
38-48
48-58
58-70.
I am getting some info on Internet that says 58 but is the range lower or 
higher than that? What do we use?

This seems to me to be an odd question because it depends on the melting point 
of the paraffin in use.  Ours melts at 58C and we embed at 60C, but we have 
also used paraffin that melts at 56C and we embedded at 58C.  Or am I missing 
something?  Does anyone have a clear cut answer to this?



Thanks everyone!

Erin

Erin Martin, Histology Supervisor

UCSF  Dermatopathology Service
415-353-7248

Confidentiality Notice
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it is addressed and may contain confidential and/or priviledged material.  Any 
review, retransmission, dissemination or other use of, or taking of any action 
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RE: [Histonet] (no subject)

[Marcum, Pamela A]

The last answer will cover most paraffins used routinely and may cover 
microwave also.  Temperatures below 58C are often melt points however; they may 
not be a complete liquid or have slightly increased viscosity.

Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Erin
Sent: Monday, September 09, 2013 8:28 AM
To: histonet
Cc: Naujokas, Agne; Meier, Melissa
Subject: [Histonet] (no subject)

Good morning all!

One of our fellows emailed me a question that she came across while studying 
for her boards:



I'm studying for my board exam and came across questions re: paraffin 
embedding.
It reads: best temperature for paraffin embedding is
38-48
48-58
58-70.
I am getting some info on Internet that says 58 but is the range lower or 
higher than that? What do we use?

This seems to me to be an odd question because it depends on the melting point 
of the paraffin in use.  Ours melts at 58C and we embed at 60C, but we have 
also used paraffin that melts at 56C and we embedded at 58C.  Or am I missing 
something?  Does anyone have a clear cut answer to this?



Thanks everyone!

Erin

Erin Martin, Histology Supervisor

UCSF  Dermatopathology Service
415-353-7248

Confidentiality Notice
The information transmitted is intended only for the person or entity to which 
it is addressed and may contain confidential and/or priviledged material.  Any 
review, retransmission, dissemination or other use of, or taking of any action 
in reliance upon, this information by persons or entities other than the 
intended recipient is prohibited.  If you receive this in error, please contact 
the sender and delete the material from any computer.
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Re: [Histonet] (no subject)

[Jennifer MacDonald]

Recommended melting point of paraffin is 2-4 degrees above the melting 
point of the paraffin.  Because we really don't see paraffins that would 
have a melting point of 46, the BEST answer would be 58-70.  Perhaps not 
what we do, but the best answer for the choices provided.



From:   Martin, Erin erin.mar...@ucsf.edu
To: histonet histonet@lists.utsouthwestern.edu
Cc: Naujokas, Agne agne.naujo...@ucsfmedctr.org, Meier, Melissa 
melissa.me...@ucsfmedctr.org
Date:   09/09/2013 06:30 AM
Subject:[Histonet] (no subject)
Sent by:histonet-boun...@lists.utsouthwestern.edu



Good morning all!

One of our fellows emailed me a question that she came across while 
studying for her boards:



I'm studying for my board exam and came across questions re: paraffin 
embedding.
It reads: best temperature for paraffin embedding is
38-48
48-58
58-70.
I am getting some info on Internet that says 58 but is the range lower or 
higher than that? What do we use?

This seems to me to be an odd question because it depends on the melting 
point of the paraffin in use.  Ours melts at 58C and we embed at 60C, but 
we have also used paraffin that melts at 56C and we embedded at 58C.  Or 
am I missing something?  Does anyone have a clear cut answer to this?



Thanks everyone!

Erin

Erin Martin, Histology Supervisor

UCSF  Dermatopathology Service
415-353-7248

Confidentiality Notice
The information transmitted is intended only for the person or entity to 
which it is addressed and may contain confidential and/or priviledged 
material.  Any review, retransmission, dissemination or other use of, or 
taking of any action in reliance upon, this information by persons or 
entities other than the intended recipient is prohibited.  If you receive 
this in error, please contact the sender and delete the material from any 
computer.
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Re: [Histonet] (no subject)

[Luis Teran]

Has anybody had issues with the scent of the slides coming off the Symphony 
auto stainer?  Our pathologists are complaining of an odor from the slides, and 
we just recently started using the Symphony.

Eileen Teran

Sent from my iPad

On Aug 7, 2013, at 5:18 PM, Jay Lundgren jaylundg...@gmail.com wrote:

 Sanjeet,
I encourage your efforts at stamping out individualism.  I have found
 that a judiciously applied cattle prod will dissuade even the most
 recalcitrant illicit forceps user.
 
  Sincerely?
 
   Jay A.
 Lundgren, M.S., HTL (ASCP)
 
 
 On Wed, Aug 7, 2013 at 10:19 AM, Grantham, Andrea L - (algranth) 
 algra...@email.arizona.edu wrote:
 
 Agree!
 I'd be lost without my own tools for working in the histo lab. I have a
 box of forceps, brushes, probes, etc. - everything that I need to work at
 the embedding station or microtome or while coverslipping. Have had these
 for too many to count years, some of the forceps came from Brookstone in
 1975!
 If your techs feel comfortable using certain tools then in my opinion,
 they will do better work.
 
 
 Andrea Grantham, HT (ASCP)
 Senior Research Specialist
 University of Arizona
 Cellular and Molecular Medicine
 Histology Service Laboratory
 P.O.Box 245044
 Tucson, AZ 85724
 
 algra...@email.arizona.edumailto:algra...@email.arizona.edu
 Tel: 520.626.4415 Fax: 520.626.2097
 
 
 
 
 
 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
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RE: [Histonet] (no subject)

[joelle weaver]

funny 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
 Date: Wed, 7 Aug 2013 16:16:53 -0500
 From: jaylundg...@gmail.com
 To: algra...@email.arizona.edu
 Subject: Re: [Histonet] (no subject)
 CC: histonet@lists.utsouthwestern.edu
 
 Sanjeet,
 I encourage your efforts at stamping out individualism.  I have found
 that a judiciously applied cattle prod will dissuade even the most
 recalcitrant illicit forceps user.
 
   Sincerely?
 
Jay A.
 Lundgren, M.S., HTL (ASCP)
 
 
 On Wed, Aug 7, 2013 at 10:19 AM, Grantham, Andrea L - (algranth) 
 algra...@email.arizona.edu wrote:
 
  Agree!
  I'd be lost without my own tools for working in the histo lab. I have a
  box of forceps, brushes, probes, etc. - everything that I need to work at
  the embedding station or microtome or while coverslipping. Have had these
  for too many to count years, some of the forceps came from Brookstone in
  1975!
  If your techs feel comfortable using certain tools then in my opinion,
  they will do better work.
 
 
  Andrea Grantham, HT (ASCP)
  Senior Research Specialist
  University of Arizona
  Cellular and Molecular Medicine
  Histology Service Laboratory
  P.O.Box 245044
  Tucson, AZ 85724
 
  algra...@email.arizona.edumailto:algra...@email.arizona.edu
  Tel: 520.626.4415 Fax: 520.626.2097
 
 
 
 
 
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  Histonet mailing list
  Histonet@lists.utsouthwestern.edu
  http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 
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 Histonet@lists.utsouthwestern.edu
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Re: [Histonet] (no subject)

[Steve Mello]

Sad commentary!
Sent via BlackBerry by ATT

-Original Message-
From: joelle weaver joellewea...@hotmail.com
Sender: histonet-boun...@lists.utsouthwestern.edu
Date: Thu, 8 Aug 2013 11:52:04 
To: Jay Lundgrenjaylundg...@gmail.com; Grantham, Andrea L - 
(algranth)algra...@email.arizona.edu
Cc: HISTONEThistonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject)

funny 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
 Date: Wed, 7 Aug 2013 16:16:53 -0500
 From: jaylundg...@gmail.com
 To: algra...@email.arizona.edu
 Subject: Re: [Histonet] (no subject)
 CC: histonet@lists.utsouthwestern.edu
 
 Sanjeet,
 I encourage your efforts at stamping out individualism.  I have found
 that a judiciously applied cattle prod will dissuade even the most
 recalcitrant illicit forceps user.
 
   Sincerely?
 
Jay A.
 Lundgren, M.S., HTL (ASCP)
 
 
 On Wed, Aug 7, 2013 at 10:19 AM, Grantham, Andrea L - (algranth) 
 algra...@email.arizona.edu wrote:
 
  Agree!
  I'd be lost without my own tools for working in the histo lab. I have a
  box of forceps, brushes, probes, etc. - everything that I need to work at
  the embedding station or microtome or while coverslipping. Have had these
  for too many to count years, some of the forceps came from Brookstone in
  1975!
  If your techs feel comfortable using certain tools then in my opinion,
  they will do better work.
 
 
  Andrea Grantham, HT (ASCP)
  Senior Research Specialist
  University of Arizona
  Cellular and Molecular Medicine
  Histology Service Laboratory
  P.O.Box 245044
  Tucson, AZ 85724
 
  algra...@email.arizona.edumailto:algra...@email.arizona.edu
  Tel: 520.626.4415 Fax: 520.626.2097
 
 
 
 
 
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  Histonet mailing list
  Histonet@lists.utsouthwestern.edu
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 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
  
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RE: [Histonet] (no subject)

[Blazek, Linda]

Hi,
I understand using lean at the embedding center but when it comes to the little 
things like the types of forceps it may be best to let the techs use what works 
best for them.  Everyone's hands are different and the feel of the forceps in 
their hands and dexterity come into play here.  If you have many different 
embedders maybe it would be best if each had and were responsible for their own 
forceps.  I don't know how that would counter affect the lean process.  

Linda

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanjeet 
Dhirubhai
Sent: Wednesday, August 07, 2013 9:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)



 Hi,

I am trying lean up at the embedding system. We have issues where staff have 
their own preference in regards to working on a specific forceps. I am trying 
to standardize this process and eliminate the hassle of having different types 
of forceps. Can anyone help me. Thanks Regards,
 
Sanjeet Dhirubhai - Supervisor Histology  MLT 
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RE: [Histonet] (no subject)

[Rathborne, Toni]

Personal preference is something we try to accommodate within reason. Each of 
our  techs have their own tools (brush, forceps, etc.) which they take care of 
and clean after each use. The more comfortable a person is with a specific 
tool, the more accurate and efficient they will likely be. If your techs use a 
forceps that is not comfortable for them, think of tiny biopsies that could be 
compromised due to that. A hand can develop a cramp and tissue could be damaged 
by too much pressure, or could pop out of the cassette and become lost if it 
wasn't grasped correctly. I don't understand how standardizing the type of 
forceps used will create a Lean process.  I would look at the bigger picture 
and remember that histology is a skill performed by people, not instruments. 
Remember to evaluate the process. If techs need improved skills for embedding, 
that should be addressed with each one that is experiencing problems.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanjeet 
Dhirubhai
Sent: Wednesday, August 07, 2013 9:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)



 Hi,

I am trying lean up at the embedding system. We have issues where staff have 
their own preference in regards to working on a specific forceps. I am trying 
to standardize this process and eliminate the hassle of having different types 
of forceps. Can anyone help me. Thanks Regards,
 
Sanjeet Dhirubhai - Supervisor Histology  MLT 
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Re: [Histonet] (no subject)

[Paula Pierce]

Ditto!
 
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
8901 S. Santa Fe, Suite G
Oklahoma City, OK 73139
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com



 From: Blazek, Linda lbla...@digestivespecialists.com
To: Sanjeet Dhirubhai asanj...@yahoo.com; histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edu 
Sent: Wednesday, August 7, 2013 8:22 AM
Subject: RE: [Histonet] (no subject)
 

Hi,
I understand using lean at the embedding center but when it comes to the little 
things like the types of forceps it may be best to let the techs use what works 
best for them.  Everyone's hands are different and the feel of the forceps in 
their hands and dexterity come into play here.  If you have many different 
embedders maybe it would be best if each had and were responsible for their own 
forceps.  I don't know how that would counter affect the lean process.  

Linda

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanjeet 
Dhirubhai
Sent: Wednesday, August 07, 2013 9:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)



 Hi,

I am trying lean up at the embedding system. We have issues where staff have 
their own preference in regards to working on a specific forceps. I am trying 
to standardize this process and eliminate the hassle of having different types 
of forceps. Can anyone help me. Thanks Regards,
 
Sanjeet Dhirubhai - Supervisor Histology  MLT 
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Re: [Histonet] (no subject)

[Paula Pierce]

Ditto!
 
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
8901 S. Santa Fe, Suite G
Oklahoma City, OK 73139
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com



 From: Rathborne, Toni trathbo...@somerset-healthcare.com
To: 'Sanjeet Dhirubhai' asanj...@yahoo.com; 
histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
Sent: Wednesday, August 7, 2013 8:26 AM
Subject: RE: [Histonet] (no subject)
 

Personal preference is something we try to accommodate within reason. Each of 
our  techs have their own tools (brush, forceps, etc.) which they take care of 
and clean after each use. The more comfortable a person is with a specific 
tool, the more accurate and efficient they will likely be. If your techs use a 
forceps that is not comfortable for them, think of tiny biopsies that could be 
compromised due to that. A hand can develop a cramp and tissue could be damaged 
by too much pressure, or could pop out of the cassette and become lost if it 
wasn't grasped correctly. I don't understand how standardizing the type of 
forceps used will create a Lean process.  I would look at the bigger picture 
and remember that histology is a skill performed by people, not instruments. 
Remember to evaluate the process. If techs need improved skills for embedding, 
that should be addressed with each one that is experiencing problems.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanjeet 
Dhirubhai
Sent: Wednesday, August 07, 2013 9:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)



 Hi,

I am trying lean up at the embedding system. We have issues where staff have 
their own preference in regards to working on a specific forceps. I am trying 
to standardize this process and eliminate the hassle of having different types 
of forceps. Can anyone help me. Thanks Regards,
 
Sanjeet Dhirubhai - Supervisor Histology  MLT 
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Re: [Histonet] (no subject)

[Steven Mello]

Totally agree.the staff need to have what works for them.   Forcing someone 
to use one type of forcep is counterproductive.   

Sent from my iPad

On Aug 7, 2013, at 10:23 AM, Paula Pierce cont...@excaliburpathology.com 
wrote:

 Ditto!
  
 Paula K. Pierce, HTL(ASCP)HT
 President
 Excalibur Pathology, Inc.
 8901 S. Santa Fe, Suite G
 Oklahoma City, OK 73139
 405-759-3953 Lab
 405-759-7513 Fax
 www.excaliburpathology.com
 
 
 
 From: Rathborne, Toni trathbo...@somerset-healthcare.com
 To: 'Sanjeet Dhirubhai' asanj...@yahoo.com; 
 histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
 Sent: Wednesday, August 7, 2013 8:26 AM
 Subject: RE: [Histonet] (no subject)
 
 
 Personal preference is something we try to accommodate within reason. Each of 
 our  techs have their own tools (brush, forceps, etc.) which they take care 
 of and clean after each use. The more comfortable a person is with a specific 
 tool, the more accurate and efficient they will likely be. If your techs use 
 a forceps that is not comfortable for them, think of tiny biopsies that could 
 be compromised due to that. A hand can develop a cramp and tissue could be 
 damaged by too much pressure, or could pop out of the cassette and become 
 lost if it wasn't grasped correctly. I don't understand how standardizing the 
 type of forceps used will create a Lean process.  I would look at the bigger 
 picture and remember that histology is a skill performed by people, not 
 instruments. Remember to evaluate the process. If techs need improved skills 
 for embedding, that should be addressed with each one that is experiencing 
 problems.
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanjeet 
 Dhirubhai
 Sent: Wednesday, August 07, 2013 9:04 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] (no subject)
 
 
 
  Hi,
 
 I am trying lean up at the embedding system. We have issues where staff have 
 their own preference in regards to working on a specific forceps. I am trying 
 to standardize this process and eliminate the hassle of having different 
 types of forceps. Can anyone help me. Thanks Regards,
  
 Sanjeet Dhirubhai - Supervisor Histology  MLT 
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RE: [Histonet] (no subject)

[Sullivan, Beatrice]

I have my embedding and cutting forceps with me today that I  acquired  shortly 
after graduation. That was many years ago but I'd be lost without them. 
Forceps, as a rule, are cheap. Let your techs use what works best for them.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Wednesday, August 07, 2013 10:24 AM
To: Blazek, Linda; Sanjeet Dhirubhai; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] (no subject)

Ditto!
 
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
8901 S. Santa Fe, Suite G
Oklahoma City, OK 73139
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com



 From: Blazek, Linda lbla...@digestivespecialists.com
To: Sanjeet Dhirubhai asanj...@yahoo.com; histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edu 
Sent: Wednesday, August 7, 2013 8:22 AM
Subject: RE: [Histonet] (no subject)
 

Hi,
I understand using lean at the embedding center but when it comes to the little 
things like the types of forceps it may be best to let the techs use what works 
best for them.  Everyone's hands are different and the feel of the forceps in 
their hands and dexterity come into play here.  If you have many different 
embedders maybe it would be best if each had and were responsible for their own 
forceps.  I don't know how that would counter affect the lean process.  

Linda

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanjeet 
Dhirubhai
Sent: Wednesday, August 07, 2013 9:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)



 Hi,

I am trying lean up at the embedding system. We have issues where staff have 
their own preference in regards to working on a specific forceps. I am trying 
to standardize this process and eliminate the hassle of having different types 
of forceps. Can anyone help me. Thanks Regards,
 
Sanjeet Dhirubhai - Supervisor Histology  MLT 
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Re: [Histonet] (no subject)

[Grantham, Andrea L - (algranth)]

Agree!
I'd be lost without my own tools for working in the histo lab. I have a box of 
forceps, brushes, probes, etc. - everything that I need to work at the 
embedding station or microtome or while coverslipping. Have had these for too 
many to count years, some of the forceps came from Brookstone in 1975!
If your techs feel comfortable using certain tools then in my opinion, they 
will do better work.


Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edumailto:algra...@email.arizona.edu
Tel: 520.626.4415 Fax: 520.626.2097





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Re: [Histonet] (no subject)

[Rena Fail]

The lab will run more efficiently if techs have forceps they are
comfortable . I had 2 pair I cut and embedded with for over 20 years. They
were longer with Thin pointy ends the tension was just right so my fingers
didn't cramp and long enough so that I could see the tiny bxs without
looking around my own hand. Though there are some people I worked with
that I miss (I've retired) and some aspects of the job I miss, I miss my
forceps most of all,
Rena Fail


On Wed, Aug 7, 2013 at 9:04 AM, Sanjeet Dhirubhai asanj...@yahoo.comwrote:



  Hi,

 I am trying lean up at the embedding system. We have issues where staff
 have their own preference in regards to working on a specific forceps. I am
 trying to standardize this process and eliminate the hassle of having
 different types of forceps. Can anyone help me. Thanks
 Regards,

 Sanjeet Dhirubhai - Supervisor Histology  MLT
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RE: [Histonet] (no subject)

[Tony Henwood (SCHN)]

And ditto again

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager  Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Thursday, 8 August 2013 12:23 AM
To: Rathborne, Toni; 'Sanjeet Dhirubhai'; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] (no subject)

Ditto!
 
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
8901 S. Santa Fe, Suite G
Oklahoma City, OK 73139
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com



 From: Rathborne, Toni trathbo...@somerset-healthcare.com
To: 'Sanjeet Dhirubhai' asanj...@yahoo.com; 
histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
Sent: Wednesday, August 7, 2013 8:26 AM
Subject: RE: [Histonet] (no subject)
 

Personal preference is something we try to accommodate within reason. Each of 
our  techs have their own tools (brush, forceps, etc.) which they take care of 
and clean after each use. The more comfortable a person is with a specific 
tool, the more accurate and efficient they will likely be. If your techs use a 
forceps that is not comfortable for them, think of tiny biopsies that could be 
compromised due to that. A hand can develop a cramp and tissue could be damaged 
by too much pressure, or could pop out of the cassette and become lost if it 
wasn't grasped correctly. I don't understand how standardizing the type of 
forceps used will create a Lean process.  I would look at the bigger picture 
and remember that histology is a skill performed by people, not instruments. 
Remember to evaluate the process. If techs need improved skills for embedding, 
that should be addressed with each one that is experiencing problems.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanjeet 
Dhirubhai
Sent: Wednesday, August 07, 2013 9:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)



 Hi,

I am trying lean up at the embedding system. We have issues where staff have 
their own preference in regards to working on a specific forceps. I am trying 
to standardize this process and eliminate the hassle of having different types 
of forceps. Can anyone help me. Thanks Regards,
 
Sanjeet Dhirubhai - Supervisor Histology  MLT 
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Views expressed in this message and any attachments are those of the individual 
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RE: [Histonet] (no subject)

[Walter Benton]

Stacey,

In addition to the 70% step, many processors (not sure about this one had ways 
to perform a warm water flush) to rinse the lines of salt precipitate.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of STACEY 
[stace...@sbcglobal.net]
Sent: Monday, July 15, 2013 1:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Our laboratory has a Leica Peloris processor. Currently, we are using 
unbuffered 10% formalin. We are in the process of switching to 10% neutral 
buffered formalin. There is some concern about salt build-up within the 
processor, as the cleaning cycle only consists of cleaning xylene and cleaning 
alcohol. I spoke to a representative at Leica who maintains that if you use 1 
or 2 stations containing 70% alcohol immediately after the formain stations, 
that this is enough to prevent salt build-up from the buffered formalin. One of 
my technicians has some concern about this procedure. I would appreciate any 
feedback on this issue, especially from another lab using the Leica Peloris 
with 10% neutral buffered formalin. Thank you.

Stacey Merica H.T.
Histology Supervisor
North Kansas City Hospital
North Kansas City, MO
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Re: [Histonet] (no subject)

[Tony Auge]

I recently was having salt percipitation problems with my VIP proccesor and
we were doing a hot water flush every week. We were starting with 90%
alcohol and heat on the first station and also using 10% NBF. We switched
to 50% in the first station and 70% in the second station. This has not
only got rid of the salt problem but our small tissue biopsies look much
better. I'm not sure what your technicians concerns are about but from my
experience lowering the concentration of the first alcohols is more gentle
for the smaller tissues but it might not processes big fatty specimens as
well. There will be also more reagent carryover from the water introduced
in the first station but is worth it in my opinion. Good Luck!


Tony Auge HTL QIHC (ASCP)
Cell: (651) 373-4768
Email: tony.a...@gmail.com
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Re: [Histonet] (no subject)

[Cynthia Robinson]

If you use anything higher than 70% alcohol after the 10% buffered formalin you 
will have salt precipitate out and cause all kinds of problems. We use 70%, 
80%, 90% alcohol in the stations after the formalin and have not seen any 
issues with our Peloris or the VIPs. 


Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robin...@mercyhealth.com


 Tony Auge tony.a...@gmail.com 07/15/2013 12:47 
I recently was having salt percipitation problems with my VIP proccesor and
we were doing a hot water flush every week. We were starting with 90%
alcohol and heat on the first station and also using 10% NBF. We switched
to 50% in the first station and 70% in the second station. This has not
only got rid of the salt problem but our small tissue biopsies look much
better. I'm not sure what your technicians concerns are about but from my
experience lowering the concentration of the first alcohols is more gentle
for the smaller tissues but it might not processes big fatty specimens as
well. There will be also more reagent carryover from the water introduced
in the first station but is worth it in my opinion. Good Luck!


Tony Auge HTL QIHC (ASCP)
Cell: (651) 373-4768
Email: tony.a...@gmail.com 
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RE: [Histonet] (no subject)

[Ruth Riisnaes]

Please remove me from the mailing list as well.

Thanks.

Ms Ruth Riisnaes
Cancer Biomarkers Team
MGN3, MUCRC
The Institute of Cancer Research
15, Cotswold Road
Sutton 
Surrey SM2 5NG

Tel. 020 8643 8901 x 4778
Fax 020 8722 4084




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of p...@dlcjax.com
Sent: 18 June 2013 14:53
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)


   Please remove me from the mailing list.



   Thanks



   Pam Mathews, CDC
   Dermatology and Laser Center
   Orange Park, Florida  32073
   Office Manager
   904-276-4500 Office
   904-276-4160 Fax
   904-945-6845 Cell
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Limited by Guarantee, Registered in England under Company No. 534147 with its 
Registered Office at 123 Old Brompton Road, London SW7 3RP.

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Re: [Histonet] (no subject)

[Emily Sours]

No.

By bitching and bitching and bitching, they could exhaust the drama of
their own horror stories. Grow bored. Only then could they accept a new
story for their lives. Move forward.

-Chuck Palahniuk, Haunted


On Tue, Jun 18, 2013 at 11:18 AM, Ruth Riisnaes ruth.riisn...@icr.ac.ukwrote:

 Please remove me from the mailing list as well.

 Thanks.

 Ms Ruth Riisnaes
 Cancer Biomarkers Team
 MGN3, MUCRC
 The Institute of Cancer Research
 15, Cotswold Road
 Sutton
 Surrey SM2 5NG

 Tel. 020 8643 8901 x 4778
 Fax 020 8722 4084




 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of p...@dlcjax.com
 Sent: 18 June 2013 14:53
 To: Histonet@lists.utsouthwestern.edu
 Subject: [Histonet] (no subject)


Please remove me from the mailing list.



Thanks



Pam Mathews, CDC
Dermatology and Laser Center
Orange Park, Florida  32073
Office Manager
904-276-4500 Office
904-276-4160 Fax
904-945-6845 Cell
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 534147 with its Registered Office at 123 Old Brompton Road, London SW7 3RP.

 This e-mail message is confidential and for use by the addressee only.  If
 the message is received by anyone other than the addressee, please return
 the message to the sender by replying to it and then delete the message
 from your computer and network.

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Re: [Histonet] (no subject)

[Jennifer Campbell]

We use India ink and then use 3% acetic acid to affix it to the specimen.
Works great on ellipse excisions as well as small slices.

Jen Campbell

On Wed, Mar 6, 2013 at 10:49 AM, Dermpath Lab d...@att.net wrote:

 Hi! I was just wondering what different facilities use for red ink/dye.
 Some of the new ones we have tried gets too light or washes off during
 processing.  Thanks!
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-- 
Jen Campbell, BS, HT(ASCP)
Supervisor of Technical Services
Muhlbauer Dermatopathology Laboratory
61 Monroe Avenue, Ste B
Pittsford NY 14534
P: 585.586.5166
F: 585.586.3137


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Re: [Histonet] (no subject)

[Rene J Buesa]

Paraplast and Feather from Sakura
René J.

From: Brendal Finlay brendal.fin...@medicalcenterclinic.com
To: histonet@lists.utsouthwestern.edu 
Sent: Wednesday, February 13, 2013 12:06 PM
Subject: [Histonet] (no subject)


Hello everyone!


I have a question for all of the histo techs out there. It appears we
are going to have to change some products and we are in need of some
product recommendations.  


Currently we use EM400 paraffin for embedding and infiltration.  Our
department has tried to change paraffin before.  The paraffins tested
did not work well for us so I would like to know if anyone can tell
me a comparable paraffin and from which vendor do you purchase?


I may also need to change the blades that I use for microtomy.  I
prefer the Surgipath / Leica high profile thin disposable blades (Item
3802123) and I am looking for a similar blade.  


Thank you!


Brendal Finlay, HT (ASCP)


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RE: [Histonet] (no subject)

[Goins, Tresa]

We recently switched to ion exchange resin with great results:

American MasterTech
I.E.D. Unit (Immuno) - Ion-Exchange Decalcification Unit 
Items: DCIEDIM60CS, DCIEDIM250CS, DCIEDIM250EA.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vikrant Piprode
Sent: Thursday, December 20, 2012 10:22 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

hi 
i want to know the best method to decalcify calvarial bone of mice for 
subsequent osteoclast staining and goldner's trichrome staining.
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RE: [Histonet] (no subject)

[Starbuck,Michael W]

If you want to do TRAP staining for osteoclasts you will need to use 10 -15% 
EDTA at neutral pH to decalcify.  It will take time but using agitation and 
changing the solution daily will facilitate the decalcification.  
Mike


Michael Starbuck, M.S.
Laboratory Coordinator
Bone Disease Program of Texas Histomorphometry Core
GU Medical Oncology
The University of Texas M. D. Anderson Cancer Center
T7.3966
ph: 713 563-1212





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vikrant Piprode
Sent: Thursday, December 20, 2012 11:22 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

hi 
i want to know the best method to decalcify calvarial bone of mice for 
subsequent osteoclast staining and goldner's trichrome staining.
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Re: [Histonet] (no subject)

[histot...@imagesbyhopper.com]

Here are some links for HT/HTL licensure in FL.
 
 
http://ww2.doh.state.fl.us/mqaservices/PractitionerServices.asp
 
 
http://www.doh.state.fl.us/mqa/ClinLab/clp_applications.html
There is a lot of information on that page, not just the application, so you 
might want to spend a few extra minutes looking around.
 
Further down on the page, you can find the link related to the application.  
I've made it easier for you, by including the direct link  here:
http://www.doh.state.fl.us/mqa/ClinLab/ap_licensure.pdf
 
The following URL is one resource for online CEUs.  You can take the classes 
online, print your certificates and mail them in with your application, all on 
the same day!
http://www.4ceuinc.com/home.asp?Profession=1
 
You will need to create a user id (using your email address is an easy way to 
remember your user name!) and password in order to see the next link. 
http://www.4ceuinc.com/courseDetails.asp?CourseId=344
 
This link takes you to the page for NEW FL licensee people.  I would suggest 
you take the online courses, so you can print your certificate at the end


On Sep 20, 2012, at 12:19 PM, Dorothy Glass techman...@yahoo.com wrote:

 What are the steps to applying and receiving a health care license to work in 
 florida for an HT or an HTL? I heard there is also a requirement or different 
 fees to be paid for a position as supervisor.
 Curious in Georgia.
 
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Re: [Histonet] (no subject)

[Rene J Buesa]

I used Kapak pouches, heat sealed with a minimum of formalin inside. Just 
enough to surround the tissues that have already been fixed.
René J.



From: Lynette Pavelich lpave...@hurleymc.com
To: Bernadette del Rosario badzros...@yahoo.com; 
histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
Sent: Monday, September 3, 2012 8:26 PM
Subject: RE: [Histonet] (no subject)

Some years ago, our morgue stored stock tissues in sealed bags. These were 
actually the stronger plastic bags that are heat-sealed closed (much thicker 
plastic than the style of bags you were speaking of).  After some time, (~2-3 
yrs), the seams of the bags weakened and leaked all over, especially if they 
were stored piled on top of each other.  It was disappointing as we were 
running out of room too. 
Additionally, we have found that  plastic containers that are stored one on top 
of another, the weight of the top container will finally crack the lid below 
and the NBF evaporates. 
I also would like to hear what others are doing for successful long termed 
preservation of tissues besides using glass jars!

Lynette



From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Bernadette del Rosario 
[badzros...@yahoo.com]
Sent: Monday, September 03, 2012 8:19 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Hi histoland..Anyone using resealable bags for storing their tissue samples 
after grossing??Coz i need a bit space for our small lab instead of storing it 
in the same container after it has been gross.Good space and easy to dispose 
later on...How and where can i order it??Any supplier???Tanx...
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Re: [Histonet] (no subject)

[Jackie O'Connor]

We use Kapak bags which are sealed with a heat sealer.  Our tissues are 
preserved for 15 years in this manner, with no leaks.  I recently had to 
process some retrieved tissues which had been in storage for 14 years - they 
were great, and the quality of the tissues was no different than if the tissues 
had been taken the day after necropsy.  Kapak bags are available through VWR in 
many different sizes.  The heat sealer is about $1000.
Jackie O'


-Original Message-
From: Lynette Pavelich lpave...@hurleymc.com
To: Bernadette del Rosario badzros...@yahoo.com; histonet 
histonet@lists.utsouthwestern.edu
Sent: Mon, Sep 3, 2012 7:27 pm
Subject: RE: [Histonet] (no subject)


Some years ago, our morgue stored stock tissues in sealed bags. These were 
actually the stronger plastic bags that are heat-sealed closed (much thicker 
plastic than the style of bags you were speaking of).  After some time, (~2-3 
yrs), the seams of the bags weakened and leaked all over, especially if they 
were stored piled on top of each other.  It was disappointing as we were 
running 
out of room too. 
Additionally, we have found that  plastic containers that are stored one on top 
of another, the weight of the top container will finally crack the lid below 
and 
the NBF evaporates. 
I also would like to hear what others are doing for successful long termed 
preservation of tissues besides using glass jars!

Lynette



From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] 
on behalf of Bernadette del Rosario [badzros...@yahoo.com]
Sent: Monday, September 03, 2012 8:19 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Hi histoland..Anyone using resealable bags for storing their tissue samples 
after grossing??Coz i need a bit space for our small lab instead of storing it 
in the same container after it has been gross.Good space and easy to dispose 
later on...How and where can i order it??Any supplier???Tanx...
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RE: [Histonet] (no subject)

[Lynette Pavelich]

Some years ago, our morgue stored stock tissues in sealed bags. These were 
actually the stronger plastic bags that are heat-sealed closed (much thicker 
plastic than the style of bags you were speaking of).  After some time, (~2-3 
yrs), the seams of the bags weakened and leaked all over, especially if they 
were stored piled on top of each other.  It was disappointing as we were 
running out of room too. 
Additionally, we have found that  plastic containers that are stored one on top 
of another, the weight of the top container will finally crack the lid below 
and the NBF evaporates. 
I also would like to hear what others are doing for successful long termed 
preservation of tissues besides using glass jars!

Lynette



From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Bernadette del Rosario 
[badzros...@yahoo.com]
Sent: Monday, September 03, 2012 8:19 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Hi histoland..Anyone using resealable bags for storing their tissue samples 
after grossing??Coz i need a bit space for our small lab instead of storing it 
in the same container after it has been gross.Good space and easy to dispose 
later on...How and where can i order it??Any supplier???Tanx...
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RE: [Histonet] (no subject)

[Blazek, Linda]

Old telephone book pages!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Tuesday, July 31, 2012 2:23 PM
To: Histonet
Subject: [Histonet] (no subject)

Greetings,

does anyone know of a less expensive substitute for Kim-wipes?

 
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
8901 S. Santa Fe, Suite G
Oklahoma City, OK 73139
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com
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RE: [Histonet] (no subject)

[Connolly, Brett M]

 Second that - learned that trick for cleaning the water bath surface from Pete 
Emanuele at AFIP a looong time ago.

Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Tuesday, July 31, 2012 3:05 PM
To: 'Paula Pierce'; Histonet
Subject: RE: [Histonet] (no subject)

Old telephone book pages!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Tuesday, July 31, 2012 2:23 PM
To: Histonet
Subject: [Histonet] (no subject)

Greetings,

does anyone know of a less expensive substitute for Kim-wipes?

 
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
8901 S. Santa Fe, Suite G
Oklahoma City, OK 73139
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com
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please notify us immediately by reply e-mail and then delete it from 
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Re: [Histonet] (no subject)

[nmhisto]

Back in the Olde Days, when we gathered wax for embedding from the beehives in 
the back of the hospital and used the eggs from the hospital's chicken coops to 
make albumin as a slide adhesive (I'm being facetious here, I hope you 
know...), we used ScotTowels, torn in half, for cleaning water bath surfaces 
while cutting.  I've sent this idea to Paula Pierce but it bears repeating here 
as I'm sure one could find very inexpensive paper towels in the pick-a-size 
package.  I've always thought Kimwipes were too wimpy for cleaning the water 
surface but I apparently was spoiled by ScotTowels!  And, yes, I am enjoying 
retirement but I rather miss the routine.  I'll get over it! 

- Original Message -
From: Linda Blazek lbla...@digestivespecialists.com 
To: Paula Pierce cont...@excaliburpathology.com, Histonet 
histonet@lists.utsouthwestern.edu 
Sent: Tuesday, July 31, 2012 1:05:23 PM 
Subject: RE: [Histonet] (no subject) 

Old telephone book pages! 

-Original Message- 
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce 
Sent: Tuesday, July 31, 2012 2:23 PM 
To: Histonet 
Subject: [Histonet] (no subject) 

Greetings, 

does anyone know of a less expensive substitute for Kim-wipes? 

  
Paula K. Pierce, HTL(ASCP)HT 
President 
Excalibur Pathology, Inc. 
8901 S. Santa Fe, Suite G 
Oklahoma City, OK 73139 
405-759-3953 Lab 
405-759-7513 Fax 
www.excaliburpathology.com 
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RE: [Histonet] (no subject)

[Wanda.Smith]

Done that!!! And old shiny magazines.

WANDA G. SMITH, HTL(ASCP)HT 
Pathology Supervisor 
TRIDENT MEDICAL CENTER 
9330 Medical Plaza Drive 
Charleston, SC  29406 
843-847-4586 
843-847-4296 fax 

This email and any files transmitted with it may contain PRIVILEGED or 
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attachments, please be advised that you have received this email in error and 
that any use, dissemination, distribution, forwarding, printing, or copying of 
this email or any attached files is strictly prohibited. If you have received 
this email in error, please immediately purge it and all attachments and notify 
the sender by reply email or contact the sender at the number listed.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Tuesday, July 31, 2012 3:05 PM
To: 'Paula Pierce'; Histonet
Subject: RE: [Histonet] (no subject)

Old telephone book pages!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Tuesday, July 31, 2012 2:23 PM
To: Histonet
Subject: [Histonet] (no subject)

Greetings,

does anyone know of a less expensive substitute for Kim-wipes?

 
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
8901 S. Santa Fe, Suite G
Oklahoma City, OK 73139
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com
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Re: [Histonet] (no subject)

[Rena Fail]

Can't beat phone books. Cheap, torn in half they are wider than a kimwipe,
you're recycling, and providing therapy in the form of stress
relief obtained tearing up phone books.

Rena
On Tue, Jul 31, 2012 at 2:23 PM, Paula Pierce 
cont...@excaliburpathology.com wrote:

 Greetings,

 does anyone know of a less expensive substitute for Kim-wipes?


 Paula K. Pierce, HTL(ASCP)HT
 President
 Excalibur Pathology, Inc.
 8901 S. Santa Fe, Suite G
 Oklahoma City, OK 73139
 405-759-3953 Lab
 405-759-7513 Fax
 www.excaliburpathology.com
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RE: [Histonet] (no subject)

[Ingles Claire]

I'd love to know too. We also use a xylene substitute.
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Brendal Finlay
Sent: Mon 7/23/2012 12:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)




Hello all!

We are budgeted to get an automated coverslipper for our department. 
When we talked about it with our service rep, he asked if we wanted a
glass or tape coverslipper.  I worked with a glass one many years ago
when they first came out and all I remember is breaking and sticking
coverslips.  I'm sure things have improved by now and was wondering
about the pros and cons of the glass versus tape coverslipper.  Which
do you prefer and why?


Thank you!
Brendal C. Finlay, HT (ASCP)
West Florida Medical Center Clinic
brendal.fin...@medicalcenterclinic.com


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RE: [Histonet] (no subject)

[Bea DeBrosse-Serra]

It all depends on how long you want to store the slides. The tape has a 
tendency to come off, whereas the glass slides, if covered properly, can be 
stored indefinitely. The glass coverslippers have improved quite a bit, and 
there are several vendors out there who have them. We have one here, and we 
love it! 
As far as xylene substitutes, you may want to check about this with the vendor.

Bea

Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire 
Sent: Monday, July 23, 2012 12:31 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject)

I'd love to know too. We also use a xylene substitute.
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Brendal Finlay
Sent: Mon 7/23/2012 12:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)




Hello all!

We are budgeted to get an automated coverslipper for our department. 
When we talked about it with our service rep, he asked if we wanted a glass or 
tape coverslipper.  I worked with a glass one many years ago when they first 
came out and all I remember is breaking and sticking coverslips.  I'm sure 
things have improved by now and was wondering about the pros and cons of the 
glass versus tape coverslipper.  Which do you prefer and why?


Thank you!
Brendal C. Finlay, HT (ASCP)
West Florida Medical Center Clinic
brendal.fin...@medicalcenterclinic.com


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Re: [Histonet] (no subject)

[betterpat...@yahoo.com]

Hi - all hands down the film. Faster,  less daily issues.  I was fortunate to 
be one if the first to try this in the Boston area in the early 90's.  I have 
done numerous studies including long term storage.  You need to ensure adequate 
'clean' xylene drips 3-5 per slide. GOOD LUCK

Candace 

Connected by DROID on Verizon Wireless

-Original message-
From: Brendal Finlay brendal.fin...@medicalcenterclinic.com
To: histonet@lists.utsouthwestern.edu
Sent: Mon, Jul 23, 2012 17:58:08 GMT+00:00
Subject: [Histonet] (no subject)


Hello all!

We are budgeted to get an automated coverslipper for our department. 
When we talked about it with our service rep, he asked if we wanted a
glass or tape coverslipper.  I worked with a glass one many years ago
when they first came out and all I remember is breaking and sticking
coverslips.  I'm sure things have improved by now and was wondering
about the pros and cons of the glass versus tape coverslipper.  Which
do you prefer and why?


Thank you!
Brendal C. Finlay, HT (ASCP)
West Florida Medical Center Clinic
brendal.fin...@medicalcenterclinic.com


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Re: [Histonet] (no subject)

[Jennifer MacDonald]

It is added to formaldehyde to prevent polymerization of the formaldehyde.




Bader Siddiki bade...@gmail.com 
Sent by: histonet-boun...@lists.utsouthwestern.edu
07/17/2012 01:44 PM

To
Rene J Buesa rjbu...@yahoo.com
cc
histonet Histonet@lists.utsouthwestern.edu, Kelly Boyd 
kdboydhi...@yahoo.com
Subject
Re: [Histonet] (no subject)






Hello
 Methanol is added to reagent alcohol (ethyl alcohol) so that people do 
not
use for drinking purpose and for tax purposes. Alcohol (ethyl alcohol) has
federal tax just like liquor.
One can buy pure alcohol, but you have keep inventory and make sure it is
not misused.
Bader

On Tue, Jul 17, 2012 at 1:28 PM, Rene J Buesa rjbu...@yahoo.com wrote:

 To prevent oxidation of those 2 reagents
 René J.


 
 From: Kelly Boyd kdboydhi...@yahoo.com
 To: histonet Histonet@lists.utsouthwestern.edu
 Sent: Tuesday, July 17, 2012 1:16 PM
 Subject: [Histonet] (no subject)

 Since there is discussion of formalin and methanol, I would like to ask
 everyone.Why would a vendor add methanol to their reagent alcohol 
and
 their 10% buffered formalin?

 What are the advantages/disadvantages to the tissue especially for
 processing?

 Kelly
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-- 
If any Q's please feel free to contact us
Have a nice day/weekend
Mit freundlichen Grüßen / With Kind Regards /
avec l'aimable ce qui concerne
Met vriendelijke groeten
種とについて
Bader
Bader B Siddiki, PhD
Executive director,
Research and development
ImmunoBioScience Corp. (IBSC)
Phone: + 1 425 367 4601
Fax: + 1 425 367 4817
cell (mobile) phone: + 1 425 314 0199
e-mail address: bade...@gmail.com
Web site: www.ImmunoBioScience.Com
Marketing: phone: + 1 650 343 IBSC (4272)
E-mail: anitai...@aol.com
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Re: [Histonet] (no subject)

[Rene J Buesa]

To prevent oxidation of those 2 reagents
René J.



From: Kelly Boyd kdboydhi...@yahoo.com
To: histonet Histonet@lists.utsouthwestern.edu 
Sent: Tuesday, July 17, 2012 1:16 PM
Subject: [Histonet] (no subject)

Since there is discussion of formalin and methanol, I would like to ask 
everyone.Why would a vendor add methanol to their reagent alcohol and their 
10% buffered formalin? 

What are the advantages/disadvantages to the tissue especially for processing?

Kelly 
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Re: [Histonet] (no subject)

[Bader Siddiki]

Hello
 Methanol is added to reagent alcohol (ethyl alcohol) so that people do not
use for drinking purpose and for tax purposes. Alcohol (ethyl alcohol) has
federal tax just like liquor.
One can buy pure alcohol, but you have keep inventory and make sure it is
not misused.
Bader

On Tue, Jul 17, 2012 at 1:28 PM, Rene J Buesa rjbu...@yahoo.com wrote:

 To prevent oxidation of those 2 reagents
 René J.


 
 From: Kelly Boyd kdboydhi...@yahoo.com
 To: histonet Histonet@lists.utsouthwestern.edu
 Sent: Tuesday, July 17, 2012 1:16 PM
 Subject: [Histonet] (no subject)

 Since there is discussion of formalin and methanol, I would like to ask
 everyone.Why would a vendor add methanol to their reagent alcohol and
 their 10% buffered formalin?

 What are the advantages/disadvantages to the tissue especially for
 processing?

 Kelly
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 http://lists.utsouthwestern.edu/mailman/listinfo/histonet




-- 
If any Q's please feel free to contact us
Have a nice day/weekend
Mit freundlichen Grüßen / With Kind Regards /
avec l'aimable ce qui concerne
Met vriendelijke groeten
種とについて
Bader
Bader B Siddiki, PhD
Executive director,
Research and development
ImmunoBioScience Corp. (IBSC)
Phone: + 1 425 367 4601
Fax: + 1 425 367 4817
cell (mobile) phone: + 1 425 314 0199
e-mail address: bade...@gmail.com
Web site: www.ImmunoBioScience.Com
Marketing: phone: + 1 650 343 IBSC (4272)
E-mail: anitai...@aol.com
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Re: [Histonet] (no subject) (Not requiring HT Certification)

[Lee Peggy Wenk]

 of Histotechnology
William Beaumont Hospital
Royal Oak, MI 48073

(The opinions expressed are my own, and do not represent Beaumont Hospital.)

-Original Message- 
From: joelle weaver

Sent: Wednesday, May 23, 2012 12:41 PM
To: tpodawi...@lrgh.org
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject)


TomI compliment your approach, and I personally really like to see when 
employers do this. I think it provides opportunity, yet incentive. Two years 
seems especially reasonable. Most places that I have known who use this 
method for new-grad hires, have allowed 1 year. It is even better when they 
provide additional training and support to the person as they are preparing. 
Having been involved in an online program and clinical curricula in the 
past, I feel from my observations that continuity with work in the 
environment is essential for most in connecting the theory with execution.




Joelle Weaver MAOM, HTL (ASCP) QIHC

From: tpodawi...@lrgh.org
To: joellewea...@hotmail.com; sherrian.mc...@va.gov; 
histonet@lists.utsouthwestern.edu

Date: Wed, 23 May 2012 10:45:52 -0400
Subject: RE: [Histonet] (no subject)

The last person I hired for a tech position, was just finishing her 
on-line program. I helped her complete that and then she was given two 
years to get her certification. This was all part of her hiring agreement, 
so she knew this coming in and completely understood that failing to 
achieve either one would affect her employment.




Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer.





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle 
weaver

Sent: Tuesday, May 22, 2012 1:43 PM
To: sherrian.mc...@va.gov; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject)


That seems to be the unfortunate situation at this time...




Joelle Weaver MAOM, HTL (ASCP) QIHC
  Date: Tue, 22 May 2012 12:39:39 -0500
 From: sherrian.mc...@va.gov
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] (no subject)

 I agree and would like to add.  This is one scenario that I have seen
 many times, where hospitals  or wherever will hire histotechs without
 certifications .  I am thinking that saves them money and they still
 have a histotech.  I have seen good histotechs  that have no
 certification and likewise some bad ones with certification.  Lately I
 have seen these  schools turn out histotechs   ,  it seems with little
 encouragement to get certified.  If places will hire them without being
 certified,  there seems little incentive (unless you are self motivated
 for more money) to move on up to certification.



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RE: [Histonet] (no subject) (Not requiring HT Certification)

[joelle weaver]

Peggy Thanks so much for posting this !! I see those job descriptions you speak 
of all the time. They actually contradict themselves within the description or 
job posting itself. Such as ask for HT/HTL certification OR 1 year acceptable 
experience, and then have education requirements of HSD or GED. There are a few 
people I guess that could be grandfathered, but wat is the certification and 
education they want/require?  Many people I have encountered working in the lab 
truly don't know the certification eligibility requirements now and think that 
OJT is still open- even as you pointed out the 7 year time elapse. I stopped 
trying to correct people's misconception on this and just direct people to the 
BOC/BOR website for the routes. I have no idea if they ever actually do it, but 
I do my best to get people to the correct information.  I agree supervisors or 
managers should be more informed on this and check before they advise people, 
but just my opinion.  I do think it is misleading to hire people and allow them 
to think that this alone can lead to their certification at this point if they 
don't also pursue the education. I have seen MANY people who fell into this 
situation and then were unable to change jobs if they needed or wanted to. I 
think only people who have ever been involved with teaching seem to know about 
NAACLS.  




Joelle Weaver MAOM, HTL (ASCP) QIHC
  From: lpw...@sbcglobal.net
 To: joellewea...@hotmail.com; tpodawi...@lrgh.org
 CC: histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] (no subject) (Not requiring HT Certification)
 Date: Thu, 24 May 2012 05:43:32 -0400
 
 I'd like to wade into this discuss with a couple of comments:
 
 LABS WANTING ONLY HIGH SCHOOL GRADUATES AND/OR NON-CERTIFIED HISTOTECHS:
 Yes, I'm still hearing about places like this. When I talk with the 
 supervisors, it's because the lab wants the person doing the histotech 
 job, but they only want to pay them at lab assistant wages. Plus, once 
 they get the people trained as histotechs, the employees can't go 
 elsewhere, because the other labs only want certified histotech, and these 
 people can't get certified as they don't have the associate degree and 
 minimum 12 hours of biology and chemistry combined as required to take the 
 ASCP HT exam. So these people end up having to stay there. (Personally, I 
 think is very unfair to the employees they hire.)
 
 LABS NOT KNOWING ABOUT THE CHANGES IN HT REQUIREMENTS:
 Even though the High School route was dropped as of Jan 1, 2005 (over 7 
 years ago), I still get emails from labs that want to hire one  of my 
 students, but their job description says high school diploma. I usually call 
 these places up, and the histology supervisor had no idea the ASCP HT high 
 school route was dropped. Someone should have told them. Even though it 
 was in every NSH in Action for the 5 years previous (that's now over 12 
 years ago), in some ASCP publications each year for the 5 years previous, 
 and on both the NSH and ASCP webpage for the 5 years previous, well, since 
 they aren't NSH or ASCP members, well, someone still should have contacted 
 them directly and let them know. Sigh.
 
 I've had employees call that they were hired after the 2005 deadline, with 
 the job description of high school graduate requirement, and were told they 
 had 2 years to get the experience required, and then they had 1 additional 
 year in which to take and pass the HT exam. And when they went to sign up to 
 take the HT exam, they discovered that the HT exam requirements had dropped 
 the high school route and now the on-the-job (OJT) requires the associate 
 degree/60 credit hours with 12 credits of bio/chem, which of course they 
 don't have. They tell me that their histology supervisor says they are going 
 to fire them, because they can't take the ASCP HT Exam. I end up talking 
 with the supervisor, and advise them to talk with their HR and Legal 
 departments, as they are the ones who advertised the high school 
 requirement, and they are the ones who hired this person without the needed 
 education. And I suggest they help with person complete an on-line NAACLS HT 
 program, several of which will take someone with the high school diploma, as 
 long as they had a biology, a chemistry, and a math class in high school.
 
 NAACLS STUDENTS TAKING THE HT (OR HTL) ASCP EXAM:
 NAACLS is the accrediting agency for HT and HTL programs. (Think CAP, but 
 for most lab training programs.) NAACLS has a long list of standards for 
 programs to follow. (Think CAP checklist.)
 
 Standard 14 G has a statement The granting of the degree or certificate 
 must not be contingent upon the student's passing any type of external 
 certification or licensure examination.
 
 (Explanation: Not all HT programs end in an associate degree. The 
 certificate refers to a certificate of completion of a program. My program, 
 for example, is hospital-based. Some students already have their degree

RE: [Histonet] (no subject) (Not requiring HT Certification)

[Bernice Frederick]

If OJT is no longer a valid route, then why can someone with a BS in biology 
and a years experience in an accredited lab be allowed to take the exam? Most 
of the people falling into said category learn OTJ  and at that learn the lab, 
not all the theory, so to me, OJT is still there since many of these people 
never went to histo school. 
Bernice

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Thursday, May 24, 2012 9:26 AM
To: lpw...@sbcglobal.net; tpodawi...@lrgh.org
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject) (Not requiring HT Certification)


Peggy Thanks so much for posting this !! I see those job descriptions you speak 
of all the time. They actually contradict themselves within the description or 
job posting itself. Such as ask for HT/HTL certification OR 1 year acceptable 
experience, and then have education requirements of HSD or GED. There are a few 
people I guess that could be grandfathered, but wat is the certification and 
education they want/require?  Many people I have encountered working in the lab 
truly don't know the certification eligibility requirements now and think that 
OJT is still open- even as you pointed out the 7 year time elapse. I stopped 
trying to correct people's misconception on this and just direct people to the 
BOC/BOR website for the routes. I have no idea if they ever actually do it, but 
I do my best to get people to the correct information.  I agree supervisors or 
managers should be more informed on this and check before they advise people, 
but just my opinion.  I do think it is misleading to hire people and allow them 
to think that this alone can lead to their certification at this point if they 
don't also pursue the education. I have seen MANY people who fell into this 
situation and then were unable to change jobs if they needed or wanted to. I 
think only people who have ever been involved with teaching seem to know about 
NAACLS.  




Joelle Weaver MAOM, HTL (ASCP) QIHC
  From: lpw...@sbcglobal.net
 To: joellewea...@hotmail.com; tpodawi...@lrgh.org
 CC: histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] (no subject) (Not requiring HT Certification)
 Date: Thu, 24 May 2012 05:43:32 -0400
 
 I'd like to wade into this discuss with a couple of comments:
 
 LABS WANTING ONLY HIGH SCHOOL GRADUATES AND/OR NON-CERTIFIED HISTOTECHS:
 Yes, I'm still hearing about places like this. When I talk with the 
 supervisors, it's because the lab wants the person doing the histotech
 job, but they only want to pay them at lab assistant wages. Plus, 
 once they get the people trained as histotechs, the employees can't 
 go elsewhere, because the other labs only want certified histotech, 
 and these people can't get certified as they don't have the associate 
 degree and minimum 12 hours of biology and chemistry combined as 
 required to take the ASCP HT exam. So these people end up having to 
 stay there. (Personally, I think is very unfair to the employees they 
 hire.)
 
 LABS NOT KNOWING ABOUT THE CHANGES IN HT REQUIREMENTS:
 Even though the High School route was dropped as of Jan 1, 2005 (over 
 7 years ago), I still get emails from labs that want to hire one  of 
 my students, but their job description says high school diploma. I 
 usually call these places up, and the histology supervisor had no idea 
 the ASCP HT high school route was dropped. Someone should have told 
 them. Even though it was in every NSH in Action for the 5 years 
 previous (that's now over 12 years ago), in some ASCP publications 
 each year for the 5 years previous, and on both the NSH and ASCP 
 webpage for the 5 years previous, well, since they aren't NSH or ASCP 
 members, well, someone still should have contacted them directly and let 
 them know. Sigh.
 
 I've had employees call that they were hired after the 2005 deadline, 
 with the job description of high school graduate requirement, and were 
 told they had 2 years to get the experience required, and then they 
 had 1 additional year in which to take and pass the HT exam. And when 
 they went to sign up to take the HT exam, they discovered that the HT 
 exam requirements had dropped the high school route and now the 
 on-the-job (OJT) requires the associate
 degree/60 credit hours with 12 credits of bio/chem, which of course 
 they don't have. They tell me that their histology supervisor says 
 they are going to fire them, because they can't take the ASCP HT Exam. 
 I end up talking with the supervisor, and advise them to talk with 
 their HR and Legal departments, as they are the ones who advertised 
 the high school requirement, and they are the ones

Re: [Histonet] (no subject) (Not requiring HT Certification)

[William]

OJT is only available to HTL's via the route you described. 

Sent from my iPhone

On May 24, 2012, at 10:45 AM, Bernice Frederick b-freder...@northwestern.edu 
wrote:

 If OJT is no longer a valid route, then why can someone with a BS in biology 
 and a years experience in an accredited lab be allowed to take the exam? Most 
 of the people falling into said category learn OTJ  and at that learn the 
 lab, not all the theory, so to me, OJT is still there since many of these 
 people never went to histo school. 
 Bernice
 
 Bernice Frederick HTL (ASCP)
 Senior Research Tech
 Pathology Core Facility
 ECOGPCO-RL
 Robert. H. Lurie Cancer Center
 Northwestern University
 710 N Fairbanks Court
 Olson 8-421
 Chicago,IL 60611
 312-503-3723
 b-freder...@northwestern.edu
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
 Sent: Thursday, May 24, 2012 9:26 AM
 To: lpw...@sbcglobal.net; tpodawi...@lrgh.org
 Cc: histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] (no subject) (Not requiring HT Certification)
 
 
 Peggy Thanks so much for posting this !! I see those job descriptions you 
 speak of all the time. They actually contradict themselves within the 
 description or job posting itself. Such as ask for HT/HTL certification OR 1 
 year acceptable experience, and then have education requirements of HSD or 
 GED. There are a few people I guess that could be grandfathered, but wat is 
 the certification and education they want/require?  Many people I have 
 encountered working in the lab truly don't know the certification eligibility 
 requirements now and think that OJT is still open- even as you pointed out 
 the 7 year time elapse. I stopped trying to correct people's misconception on 
 this and just direct people to the BOC/BOR website for the routes. I have no 
 idea if they ever actually do it, but I do my best to get people to the 
 correct information.  I agree supervisors or managers should be more informed 
 on this and check before they advise people, but just my opinion.  I do think 
 it is misleading to hire people and allow them to think that this alone can 
 lead to their certification at this point if they don't also pursue the 
 education. I have seen MANY people who fell into this situation and then were 
 unable to change jobs if they needed or wanted to. I think only people who 
 have ever been involved with teaching seem to know about NAACLS.  
 
 
 
 
 Joelle Weaver MAOM, HTL (ASCP) QIHC
 From: lpw...@sbcglobal.net
 To: joellewea...@hotmail.com; tpodawi...@lrgh.org
 CC: histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] (no subject) (Not requiring HT Certification)
 Date: Thu, 24 May 2012 05:43:32 -0400
 
 I'd like to wade into this discuss with a couple of comments:
 
 LABS WANTING ONLY HIGH SCHOOL GRADUATES AND/OR NON-CERTIFIED HISTOTECHS:
 Yes, I'm still hearing about places like this. When I talk with the 
 supervisors, it's because the lab wants the person doing the histotech
 job, but they only want to pay them at lab assistant wages. Plus, 
 once they get the people trained as histotechs, the employees can't 
 go elsewhere, because the other labs only want certified histotech, 
 and these people can't get certified as they don't have the associate 
 degree and minimum 12 hours of biology and chemistry combined as 
 required to take the ASCP HT exam. So these people end up having to 
 stay there. (Personally, I think is very unfair to the employees they 
 hire.)
 
 LABS NOT KNOWING ABOUT THE CHANGES IN HT REQUIREMENTS:
 Even though the High School route was dropped as of Jan 1, 2005 (over 
 7 years ago), I still get emails from labs that want to hire one  of 
 my students, but their job description says high school diploma. I 
 usually call these places up, and the histology supervisor had no idea 
 the ASCP HT high school route was dropped. Someone should have told 
 them. Even though it was in every NSH in Action for the 5 years 
 previous (that's now over 12 years ago), in some ASCP publications 
 each year for the 5 years previous, and on both the NSH and ASCP 
 webpage for the 5 years previous, well, since they aren't NSH or ASCP 
 members, well, someone still should have contacted them directly and let 
 them know. Sigh.
 
 I've had employees call that they were hired after the 2005 deadline, 
 with the job description of high school graduate requirement, and were 
 told they had 2 years to get the experience required, and then they 
 had 1 additional year in which to take and pass the HT exam. And when 
 they went to sign up to take the HT exam, they discovered that the HT 
 exam requirements had dropped the high school route and now the 
 on-the-job (OJT) requires the associate
 degree/60 credit hours with 12 credits of bio/chem, which of course 
 they don't have. They tell me that their histology supervisor says 
 they are going to fire them, because

RE: [Histonet] (no subject) (Not requiring HT Certification)

[Bernice Frederick]

So why was OJT supposedly off the charts in 2005 (so to speak). Guess not.

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu


-Original Message-
From: William [mailto:cha...@yahoo.com] 
Sent: Thursday, May 24, 2012 9:50 AM
To: Bernice Frederick
Cc: joelle weaver; lpw...@sbcglobal.net; tpodawi...@lrgh.org; 
histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] (no subject) (Not requiring HT Certification)

OJT is only available to HTL's via the route you described. 

Sent from my iPhone

On May 24, 2012, at 10:45 AM, Bernice Frederick b-freder...@northwestern.edu 
wrote:

 If OJT is no longer a valid route, then why can someone with a BS in biology 
 and a years experience in an accredited lab be allowed to take the exam? Most 
 of the people falling into said category learn OTJ  and at that learn the 
 lab, not all the theory, so to me, OJT is still there since many of these 
 people never went to histo school. 
 Bernice
 
 Bernice Frederick HTL (ASCP)
 Senior Research Tech
 Pathology Core Facility
 ECOGPCO-RL
 Robert. H. Lurie Cancer Center
 Northwestern University
 710 N Fairbanks Court
 Olson 8-421
 Chicago,IL 60611
 312-503-3723
 b-freder...@northwestern.edu
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle 
 weaver
 Sent: Thursday, May 24, 2012 9:26 AM
 To: lpw...@sbcglobal.net; tpodawi...@lrgh.org
 Cc: histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] (no subject) (Not requiring HT Certification)
 
 
 Peggy Thanks so much for posting this !! I see those job descriptions you 
 speak of all the time. They actually contradict themselves within the 
 description or job posting itself. Such as ask for HT/HTL certification OR 1 
 year acceptable experience, and then have education requirements of HSD or 
 GED. There are a few people I guess that could be grandfathered, but wat is 
 the certification and education they want/require?  Many people I have 
 encountered working in the lab truly don't know the certification eligibility 
 requirements now and think that OJT is still open- even as you pointed out 
 the 7 year time elapse. I stopped trying to correct people's misconception on 
 this and just direct people to the BOC/BOR website for the routes. I have no 
 idea if they ever actually do it, but I do my best to get people to the 
 correct information.  I agree supervisors or managers should be more informed 
 on this and check before they advise people, but just my opinion.  I do think 
 it is misleading to hire people and allow them to think that this alone can 
 lead to their certification at this point if they don't also pursue the 
 education. I have seen MANY people who fell into this situation and then were 
 unable to change jobs if they needed or wanted to. I think only people who 
 have ever been involved with teaching seem to know about NAACLS.  
 
 
 
 
 Joelle Weaver MAOM, HTL (ASCP) QIHC
 From: lpw...@sbcglobal.net
 To: joellewea...@hotmail.com; tpodawi...@lrgh.org
 CC: histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] (no subject) (Not requiring HT Certification)
 Date: Thu, 24 May 2012 05:43:32 -0400
 
 I'd like to wade into this discuss with a couple of comments:
 
 LABS WANTING ONLY HIGH SCHOOL GRADUATES AND/OR NON-CERTIFIED HISTOTECHS:
 Yes, I'm still hearing about places like this. When I talk with the 
 supervisors, it's because the lab wants the person doing the histotech
 job, but they only want to pay them at lab assistant wages. Plus, 
 once they get the people trained as histotechs, the employees can't 
 go elsewhere, because the other labs only want certified histotech, 
 and these people can't get certified as they don't have the associate 
 degree and minimum 12 hours of biology and chemistry combined as 
 required to take the ASCP HT exam. So these people end up having to 
 stay there. (Personally, I think is very unfair to the employees they
 hire.)
 
 LABS NOT KNOWING ABOUT THE CHANGES IN HT REQUIREMENTS:
 Even though the High School route was dropped as of Jan 1, 2005 (over
 7 years ago), I still get emails from labs that want to hire one  of 
 my students, but their job description says high school diploma. I 
 usually call these places up, and the histology supervisor had no 
 idea the ASCP HT high school route was dropped. Someone should have 
 told them. Even though it was in every NSH in Action for the 5 
 years previous (that's now over 12 years ago), in some ASCP 
 publications each year for the 5 years previous, and on both the NSH 
 and ASCP webpage for the 5 years previous, well, since they aren't 
 NSH or ASCP members, well, someone still should have contacted them 
 directly and let them know. Sigh.
 
 I've had employees call that they were

RE: [Histonet] (no subject) (Not requiring HT Certification)

[Elizabeth Chlipala]

Bernice

I hire individuals with BS and train them in the lab and then they sit for the 
HT or HTL registry after one year of employment.  I have done this consistently 
over the years.  I have had probably about 8 or so individuals train with me 
and then pass the registry.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 881-0763 cell
(303) 682-9060 fax
l...@premierlab.com

Ship to address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick 
[b-freder...@northwestern.edu]
Sent: Thursday, May 24, 2012 8:45 AM
To: joelle weaver; lpw...@sbcglobal.net; tpodawi...@lrgh.org
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject) (Not requiring HT Certification)

If OJT is no longer a valid route, then why can someone with a BS in biology 
and a years experience in an accredited lab be allowed to take the exam? Most 
of the people falling into said category learn OTJ  and at that learn the lab, 
not all the theory, so to me, OJT is still there since many of these people 
never went to histo school.
Bernice

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Thursday, May 24, 2012 9:26 AM
To: lpw...@sbcglobal.net; tpodawi...@lrgh.org
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject) (Not requiring HT Certification)


Peggy Thanks so much for posting this !! I see those job descriptions you speak 
of all the time. They actually contradict themselves within the description or 
job posting itself. Such as ask for HT/HTL certification OR 1 year acceptable 
experience, and then have education requirements of HSD or GED. There are a few 
people I guess that could be grandfathered, but wat is the certification and 
education they want/require?  Many people I have encountered working in the lab 
truly don't know the certification eligibility requirements now and think that 
OJT is still open- even as you pointed out the 7 year time elapse. I stopped 
trying to correct people's misconception on this and just direct people to the 
BOC/BOR website for the routes. I have no idea if they ever actually do it, but 
I do my best to get people to the correct information.  I agree supervisors or 
managers should be more informed on this and check before they advise people, 
but just my opinion.  I do think it is misleading to hire people and allow them 
to think that this alone can lead to their certification at this point if they 
don't also pursue the education. I have seen MANY people who fell into this 
situation and then were unable to change jobs if they needed or wanted to. I 
think only people who have ever been involved with teaching seem to know about 
NAACLS.




Joelle Weaver MAOM, HTL (ASCP) QIHC
  From: lpw...@sbcglobal.net
 To: joellewea...@hotmail.com; tpodawi...@lrgh.org
 CC: histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] (no subject) (Not requiring HT Certification)
 Date: Thu, 24 May 2012 05:43:32 -0400

 I'd like to wade into this discuss with a couple of comments:

 LABS WANTING ONLY HIGH SCHOOL GRADUATES AND/OR NON-CERTIFIED HISTOTECHS:
 Yes, I'm still hearing about places like this. When I talk with the
 supervisors, it's because the lab wants the person doing the histotech
 job, but they only want to pay them at lab assistant wages. Plus,
 once they get the people trained as histotechs, the employees can't
 go elsewhere, because the other labs only want certified histotech,
 and these people can't get certified as they don't have the associate
 degree and minimum 12 hours of biology and chemistry combined as
 required to take the ASCP HT exam. So these people end up having to
 stay there. (Personally, I think is very unfair to the employees they
 hire.)

 LABS NOT KNOWING ABOUT THE CHANGES IN HT REQUIREMENTS:
 Even though the High School route was dropped as of Jan 1, 2005 (over
 7 years ago), I still get emails from labs that want to hire one  of
 my students, but their job description says high school diploma. I
 usually call these places up, and the histology supervisor had no idea
 the ASCP HT high school route was dropped. Someone should have told
 them. Even though it was in every NSH in Action for the 5 years
 previous (that's now over 12 years ago), in some ASCP publications
 each year for the 5 years previous, and on both the NSH and ASCP
 webpage for the 5 years previous, well, since they aren't NSH or ASCP
 members, well, someone still should

RE: [Histonet] (no subject) (Not requiring HT Certification)

[Morken, Timothy]

Bernice, I think you are confusing OJT with education requirements. The 
Education requirements changed, not the route of training. And the education 
requirements are simply for basic  math and science, not specifically for 
Histotechnology. Since histotech schools are so rare the vast majority of 
histotechs are still trained on the job. We just hired a person this week who 
came into the lab several months ago as a temp lab assistant for basic 
non-histo work and had no clue about histotechnology. However she showed 
excellent trainability, has a B.S. degree and became very interested in the 
work we do. We hired her as a full time regular general lab tech (not a 
histotech) with the intention of training her in to histology. She will qualify 
to take the HTL in the next couple years.


Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
San Francisco, CA





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice 
Frederick
Sent: Thursday, May 24, 2012 7:52 AM
To: William
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject) (Not requiring HT Certification)

So why was OJT supposedly off the charts in 2005 (so to speak). Guess not.

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu


-Original Message-
From: William [mailto:cha...@yahoo.com]
Sent: Thursday, May 24, 2012 9:50 AM
To: Bernice Frederick
Cc: joelle weaver; lpw...@sbcglobal.net; tpodawi...@lrgh.org; 
histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] (no subject) (Not requiring HT Certification)

OJT is only available to HTL's via the route you described.

Sent from my iPhone

On May 24, 2012, at 10:45 AM, Bernice Frederick b-freder...@northwestern.edu 
wrote:

 If OJT is no longer a valid route, then why can someone with a BS in biology 
 and a years experience in an accredited lab be allowed to take the exam? Most 
 of the people falling into said category learn OTJ  and at that learn the 
 lab, not all the theory, so to me, OJT is still there since many of these 
 people never went to histo school.
 Bernice

 Bernice Frederick HTL (ASCP)
 Senior Research Tech
 Pathology Core Facility
 ECOGPCO-RL
 Robert. H. Lurie Cancer Center
 Northwestern University
 710 N Fairbanks Court
 Olson 8-421
 Chicago,IL 60611
 312-503-3723
 b-freder...@northwestern.edu


 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle
 weaver
 Sent: Thursday, May 24, 2012 9:26 AM
 To: lpw...@sbcglobal.net; tpodawi...@lrgh.org
 Cc: histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] (no subject) (Not requiring HT Certification)


 Peggy Thanks so much for posting this !! I see those job descriptions you 
 speak of all the time. They actually contradict themselves within the 
 description or job posting itself. Such as ask for HT/HTL certification OR 1 
 year acceptable experience, and then have education requirements of HSD or 
 GED. There are a few people I guess that could be grandfathered, but wat is 
 the certification and education they want/require?  Many people I have 
 encountered working in the lab truly don't know the certification eligibility 
 requirements now and think that OJT is still open- even as you pointed out 
 the 7 year time elapse. I stopped trying to correct people's misconception on 
 this and just direct people to the BOC/BOR website for the routes. I have no 
 idea if they ever actually do it, but I do my best to get people to the 
 correct information.  I agree supervisors or managers should be more informed 
 on this and check before they advise people, but just my opinion.  I do think 
 it is misleading to hire people and allow them to think that this alone can 
 lead to their certification at this point if they don't also pursue the 
 education. I have seen MANY people who fell into this situation and then were 
 unable to change jobs if they needed or wanted to. I think only people who 
 have ever been involved with teaching seem to know about NAACLS.




 Joelle Weaver MAOM, HTL (ASCP) QIHC
 From: lpw...@sbcglobal.net
 To: joellewea...@hotmail.com; tpodawi...@lrgh.org
 CC: histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] (no subject) (Not requiring HT Certification)
 Date: Thu, 24 May 2012 05:43:32 -0400

 I'd like to wade into this discuss with a couple of comments:

 LABS WANTING ONLY HIGH SCHOOL GRADUATES AND/OR NON-CERTIFIED HISTOTECHS:
 Yes, I'm still hearing about places like this. When I talk with the
 supervisors, it's because the lab wants the person doing the histotech
 job, but they only want to pay them at lab assistant wages. Plus

RE: [Histonet] (no subject) (Not requiring HT Certification)

[joelle weaver]

I think that the original post was referring to people with a HSD or GED. I am 
not sure of the exact reasons considered in discontinuation of OJT route, but I 
recall some publications discussing the desire to raise the perception and 
awareness of the profession. The people with bachelors and the science credits 
needed can choose either the HT or HTL as I understand it, with the one year of 
verified experience/training. I think there is a route with a associate's and 
the required science credits available with the verified training for the HT. I 
believe there are now two routes for each exam if I recall correctly, one being 
the program graduate route  plus experience , and one with education 
requirements met and training/experience. There is a page on the site that lays 
this out if anyone wants to clarify/correct my recollection. 




Joelle Weaver MAOM, HTL (ASCP) QIHC
  CC: joellewea...@hotmail.com; lpw...@sbcglobal.net; tpodawi...@lrgh.org; 
  histonet@lists.utsouthwestern.edu
 From: cha...@yahoo.com
 Subject: Re: [Histonet] (no subject) (Not requiring HT Certification)
 Date: Thu, 24 May 2012 10:50:23 -0400
 To: b-freder...@northwestern.edu
 
 OJT is only available to HTL's via the route you described. 
 
 Sent from my iPhone
 
 On May 24, 2012, at 10:45 AM, Bernice Frederick 
 b-freder...@northwestern.edu wrote:
 
  If OJT is no longer a valid route, then why can someone with a BS in 
  biology and a years experience in an accredited lab be allowed to take the 
  exam? Most of the people falling into said category learn OTJ  and at that 
  learn the lab, not all the theory, so to me, OJT is still there since many 
  of these people never went to histo school. 
  Bernice
  
  Bernice Frederick HTL (ASCP)
  Senior Research Tech
  Pathology Core Facility
  ECOGPCO-RL
  Robert. H. Lurie Cancer Center
  Northwestern University
  710 N Fairbanks Court
  Olson 8-421
  Chicago,IL 60611
  312-503-3723
  b-freder...@northwestern.edu
  
  
  -Original Message-
  From: histonet-boun...@lists.utsouthwestern.edu 
  [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle 
  weaver
  Sent: Thursday, May 24, 2012 9:26 AM
  To: lpw...@sbcglobal.net; tpodawi...@lrgh.org
  Cc: histonet@lists.utsouthwestern.edu
  Subject: RE: [Histonet] (no subject) (Not requiring HT Certification)
  
  
  Peggy Thanks so much for posting this !! I see those job descriptions you 
  speak of all the time. They actually contradict themselves within the 
  description or job posting itself. Such as ask for HT/HTL certification OR 
  1 year acceptable experience, and then have education requirements of HSD 
  or GED. There are a few people I guess that could be grandfathered, but wat 
  is the certification and education they want/require?  Many people I have 
  encountered working in the lab truly don't know the certification 
  eligibility requirements now and think that OJT is still open- even as you 
  pointed out the 7 year time elapse. I stopped trying to correct people's 
  misconception on this and just direct people to the BOC/BOR website for the 
  routes. I have no idea if they ever actually do it, but I do my best to get 
  people to the correct information.  I agree supervisors or managers should 
  be more informed on this and check before they advise people, but just my 
  opinion.  I do think it is misleading to hire people and allow them to 
  think that this alone can lead to their certification at this point if they 
  don't also pursue the education. I have seen MANY people who fell into this 
  situation and then were unable to change jobs if they needed or wanted to. 
  I think only people who have ever been involved with teaching seem to know 
  about NAACLS.  
  
  
  
  
  Joelle Weaver MAOM, HTL (ASCP) QIHC
  From: lpw...@sbcglobal.net
  To: joellewea...@hotmail.com; tpodawi...@lrgh.org
  CC: histonet@lists.utsouthwestern.edu
  Subject: Re: [Histonet] (no subject) (Not requiring HT Certification)
  Date: Thu, 24 May 2012 05:43:32 -0400
  
  I'd like to wade into this discuss with a couple of comments:
  
  LABS WANTING ONLY HIGH SCHOOL GRADUATES AND/OR NON-CERTIFIED HISTOTECHS:
  Yes, I'm still hearing about places like this. When I talk with the 
  supervisors, it's because the lab wants the person doing the histotech
  job, but they only want to pay them at lab assistant wages. Plus, 
  once they get the people trained as histotechs, the employees can't 
  go elsewhere, because the other labs only want certified histotech, 
  and these people can't get certified as they don't have the associate 
  degree and minimum 12 hours of biology and chemistry combined as 
  required to take the ASCP HT exam. So these people end up having to 
  stay there. (Personally, I think is very unfair to the employees they 
  hire.)
  
  LABS NOT KNOWING ABOUT THE CHANGES IN HT REQUIREMENTS:
  Even though the High School route was dropped as of Jan 1, 2005 (over 
  7 years ago), I still get emails from

Re: [Histonet] (no subject) (Not requiring HT Certification)

[Jon Krupp]

On May 24, 2012, at 9:25 AM, joelle weaver wrote:

 
 I think that the original post was referring to people with a HSD or GED. I 
 am not sure of the exact reasons considered in discontinuation of OJT route, 
 but I recall some publications discussing the desire to raise the perception 
 and awareness of the profession. The people with bachelors and the science 
 credits needed can choose either the HT or HTL as I understand it, with the 
 one year of verified experience/training. I think there is a route with a 
 associate's and the required science credits available with the verified 
 training for the HT. I believe there are now two routes for each exam if I 
 recall correctly, one being the program graduate route  plus experience , and 
 one with education requirements met and training/experience. There is a page 
 on the site that lays this out if anyone wants to clarify/correct my 
 recollection. 

OK, so I have a question.

We train students to do electron microscopy, both specimen prep and instrument 
operation.  They fix and embed (in plastic) tissues and make thick sections (1 
um or less for us) and examine them using LM. It is not much of a leap to add 
paraffin techniques and/or basic staining etc. We have most of the equipment 
that would be needed already, but I am not ready to go into a full blown HT 
curriculum.

So, the question is, if a student gets an Associates degree that includes the 
basic science, would it help the student to get the basics of HT before looking 
for a job? Or could they take the test, get something to show for their work 
and make them a good job candidate?

Our students are skilled and could do the job, but figuring out how to help 
them and give them the right advice is my problem now.

Jon

Jonathan Krupp
Delta College
5151 Pacific Ave.
Box 212
Stockton, CA  95207
209-954-5284
jkr...@deltacollege.edu

Find us on Facebook @
Electron Microscopy at SJ Delta College







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RE: [Histonet] (no subject) (Not requiring HT Certification)

[joelle weaver]

Jon There is a route with associates and training I believe. 
Of course I can't speak for the BOC, and I am sure that you want to help your 
employees as much as you can. I do see your point about the similarities in 
tasks. My thought would be that the exam eligibility states that they have to 
have recent experience in fixation, embedding, microtomy, and staining 
(histology) and the associated theory knowledge. EM is on the exam study 
topics, but also with the theory/experience for all those routine histological 
techniques, is how I read it. Take a look at the exam outlines, that should 
give you an idea of the scope. Ascp.org get certified.  As I have been told, 
they want to cover the widest possible scope of roles histologists can perform, 
which could include EM, but not only that. If they don't have exposure to 
regular histology I think that it might be hard for to feel prepared for the 
regular HT or HTL exams. That's just my opinion, based on what I have observed 
and also the pass rates ( ~ 65%), for people even with training/experience- 
there could be an exceptional person out there.   I can understand not wanting 
to get buried in doing a whole HT curricula ( believe me, I do). How about the 
option of having cross training in a histology lab? Do you have routine 
histology on site or a nearby lab?  The best advice I can give is to go to the 
website and carefully read the requirments to see how your employees might fit 
in. If you want to provide the theory without having to do the curricula, there 
are on line programs out there which can supplement OJT and a supportive mentor 
and organization. I have seen this work successfully with motivated people with 
the ability to have hands on practice alongside. I suggest the NSH site which 
lists the accredited programs  or the NAACLS site which  has a search for 
programs, if that would help.  As far as employability, my opinion is that it 
would certainly open up options for your employees to also have skills in 
routine histology make them more valuable to your organization, and I would 
think certification would be even more helpful to them as far as options. 



Joelle Weaver MAOM, HTL (ASCP) QIHC
  Subject: Re: [Histonet] (no subject) (Not requiring HT Certification)
 From: jkr...@deltacollege.edu
 Date: Thu, 24 May 2012 09:53:21 -0700
 CC: histonet@lists.utsouthwestern.edu
 To: joellewea...@hotmail.com
 
 
 On May 24, 2012, at 9:25 AM, joelle weaver wrote:
 
  
  I think that the original post was referring to people with a HSD or GED. I 
  am not sure of the exact reasons considered in discontinuation of OJT 
  route, but I recall some publications discussing the desire to raise the 
  perception and awareness of the profession. The people with bachelors and 
  the science credits needed can choose either the HT or HTL as I understand 
  it, with the one year of verified experience/training. I think there is a 
  route with a associate's and the required science credits available with 
  the verified training for the HT. I believe there are now two routes for 
  each exam if I recall correctly, one being the program graduate route  plus 
  experience , and one with education requirements met and 
  training/experience. There is a page on the site that lays this out if 
  anyone wants to clarify/correct my recollection. 
 
 OK, so I have a question.
 
 We train students to do electron microscopy, both specimen prep and 
 instrument operation.  They fix and embed (in plastic) tissues and make thick 
 sections (1 um or less for us) and examine them using LM. It is not much of a 
 leap to add paraffin techniques and/or basic staining etc. We have most of 
 the equipment that would be needed already, but I am not ready to go into a 
 full blown HT curriculum.
 
 So, the question is, if a student gets an Associates degree that includes the 
 basic science, would it help the student to get the basics of HT before 
 looking for a job? Or could they take the test, get something to show for 
 their work and make them a good job candidate?
 
 Our students are skilled and could do the job, but figuring out how to help 
 them and give them the right advice is my problem now.
 
 Jon
 
 Jonathan Krupp
 Delta College
 5151 Pacific Ave.
 Box 212
 Stockton, CA  95207
 209-954-5284
 jkr...@deltacollege.edu
 
 Find us on Facebook @
 Electron Microscopy at SJ Delta College
 
 
 
 
 
 
  
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RE: [Histonet] (no subject)

[Podawiltz, Thomas]

The last person I hired for a tech position, was just finishing her on-line 
program. I helped her complete that and then she was given two years to get her 
certification. This was all part of her hiring agreement, so she knew this 
coming in and completely understood that failing to achieve either one would 
affect her employment.



Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
 
 

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Tuesday, May 22, 2012 1:43 PM
To: sherrian.mc...@va.gov; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject)


That seems to be the unfortunate situation at this time...




Joelle Weaver MAOM, HTL (ASCP) QIHC
  Date: Tue, 22 May 2012 12:39:39 -0500
 From: sherrian.mc...@va.gov
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] (no subject)
 
 I agree and would like to add.  This is one scenario that I have seen
 many times, where hospitals  or wherever will hire histotechs without
 certifications .  I am thinking that saves them money and they still
 have a histotech.  I have seen good histotechs  that have no
 certification and likewise some bad ones with certification.  Lately I
 have seen these  schools turn out histotechs   ,  it seems with little
 encouragement to get certified.  If places will hire them without being
 certified,  there seems little incentive (unless you are self motivated
 for more money) to move on up to certification.
 
  
 
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RE: [Histonet] (no subject)

[joelle weaver]

TomI compliment your approach, and I personally really like to see when 
employers do this. I think it provides opportunity, yet incentive. Two years 
seems especially reasonable. Most places that I have known who use this method 
for new-grad hires, have allowed 1 year. It is even better when they provide 
additional training and support to the person as they are preparing. Having 
been involved in an online program and clinical curricula in the past, I feel 
from my observations that continuity with work in the environment is essential 
for most in connecting the theory with execution. 



Joelle Weaver MAOM, HTL (ASCP) QIHC
  From: tpodawi...@lrgh.org
 To: joellewea...@hotmail.com; sherrian.mc...@va.gov; 
 histonet@lists.utsouthwestern.edu
 Date: Wed, 23 May 2012 10:45:52 -0400
 Subject: RE: [Histonet] (no subject)
 
 The last person I hired for a tech position, was just finishing her on-line 
 program. I helped her complete that and then she was given two years to get 
 her certification. This was all part of her hiring agreement, so she knew 
 this coming in and completely understood that failing to achieve either one 
 would affect her employment.
 
 
 
 Tom Podawiltz HT (ASCP)
 Histology Section Head/Laboratory Safety Officer. 
  
  
 
  
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
 Sent: Tuesday, May 22, 2012 1:43 PM
 To: sherrian.mc...@va.gov; histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] (no subject)
 
 
 That seems to be the unfortunate situation at this time...
 
 
 
 
 Joelle Weaver MAOM, HTL (ASCP) QIHC
   Date: Tue, 22 May 2012 12:39:39 -0500
  From: sherrian.mc...@va.gov
  To: histonet@lists.utsouthwestern.edu
  Subject: [Histonet] (no subject)
  
  I agree and would like to add.  This is one scenario that I have seen
  many times, where hospitals  or wherever will hire histotechs without
  certifications .  I am thinking that saves them money and they still
  have a histotech.  I have seen good histotechs  that have no
  certification and likewise some bad ones with certification.  Lately I
  have seen these  schools turn out histotechs   ,  it seems with little
  encouragement to get certified.  If places will hire them without being
  certified,  there seems little incentive (unless you are self motivated
  for more money) to move on up to certification.
  
   
  
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 information intended only for the use of the recipient(s) named above. If you 
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 message or any attachments.  If you have received this communication in 
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 any attachments from your computer. Any views or opinions expressed are 
 solely those of the author and do not necessarily represent those of 
 LRGHealthcare.
 
  
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RE: [Histonet] (no subject)

[joelle weaver]

That seems to be the unfortunate situation at this time...




Joelle Weaver MAOM, HTL (ASCP) QIHC
  Date: Tue, 22 May 2012 12:39:39 -0500
 From: sherrian.mc...@va.gov
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] (no subject)
 
 I agree and would like to add.  This is one scenario that I have seen
 many times, where hospitals  or wherever will hire histotechs without
 certifications .  I am thinking that saves them money and they still
 have a histotech.  I have seen good histotechs  that have no
 certification and likewise some bad ones with certification.  Lately I
 have seen these  schools turn out histotechs   ,  it seems with little
 encouragement to get certified.  If places will hire them without being
 certified,  there seems little incentive (unless you are self motivated
 for more money) to move on up to certification.
 
  
 
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 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
  
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RE: [Histonet] (no subject)

[O'Donnell, Bill]

 Am considering a job change. 30 years histology experience, bench and
supervisor. (HT)ASCP, QIHC
Please do not respond in the histo-net, but to my private e-mail
b...@hphisto.com 

Bill

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Miha
Tesar
Sent: Tuesday, May 08, 2012 12:58 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Hi!
Please erase me from your mailing list!
Thank you best regards Miha
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Re: [Histonet] (no subject)

[Richard Cartun]

Complete loss.  You can see patchy immunoreactivity in tumors with these 
markers. 

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


 Freeman, Carol carol.free...@utoledo.edu 2/16/2012 9:56 AM 


Good Morning Histonet,

Ok, We are just starting up with MSI testing in our facility and a
question has popped up about diagnosis of these slides...  Are you
looking for a COMPLETE LOSS of this protein in the tumor, or just a loss
of some expression.?  So if you have a normal piece of tissue staining
uniform and then a tumor of that same tissue showing vague patchy
staining in some cells and a loss in other cells of interest are you
considering that a loss or not.   Does the tumor have to show a COMPLETE
loss of this protein...  Any answer is appreciated.

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Re: [Histonet] (no subject)

[Rene J Buesa]

About not using diastase for PASD because there are active enzymes in the 
tissue: that is wrong because those are the same enzymes active in any tissue 
just before it is fixed. Also remember that you are going to fix with EthOL. 
The whole issue behind the PASD (as you well know) is to compare the results of 
the PAS with and without diastase, so your co-worker is wrong.
As to not heating the silver for GMS I do see any reason to not doing that. If 
you do not heat it you will get something different to GMS. Perhaps your 
co-worker is fearful that the section may peel-off because of the heat but, 
other than that, there is no reason.
René J.
 
 
 
 
--- On Tue, 2/14/12, Ingles Claire cing...@uwhealth.org wrote:


From: Ingles Claire cing...@uwhealth.org
Subject: [Histonet] (no subject)
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, February 14, 2012, 2:22 PM


Hey histo gurus:
Just a little twist for you. We are planning on doing GMS and PASD on frozen 
skin sections. Is there any change in staining protocol for paraffin vs frozen. 
We plan on fixing in ETOH. I have a co-worker saying that we don't need to use 
diastase on the PASD because there are already active enzymes in the tissue 
as it is fresh. He also said that we don't need to heat the silver for the GMS. 
Not sure why. These changes don't seem right to me. But I have been wrong 
before (Yes, I admit it). Thanks for all the wisdom out there floating in the 
electrons. We are staining for fungus BTW.
Claire

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RE: [Histonet] (no subject)

[Tony Henwood (SCHN)]

The reason for using diastase is to remove the glycogen from the tissues. AND 
if you do not heat the methenamine silver solution for the GMS it will take 
forever to stain, in fact all you will get is background spots. This I can 
verify, have you ever forgot to turn on the water bath before staining? - 
several hours later - nuthin!!

Who ever is giving you the advice I would wonder about their histotechnology 
education and training (in fact have they had any training at all?).

Do not listen to them. Extrapolate from cytology. GMS and DiPAS have been done 
on cytology smears fixed in ethanol as well as air-dried for years see:

Loughman NT. Pneumocystis carinii: rapid diagnosis with the microwave oven 
Acta Cytologica. 33(3):416-7, 1989 May-Jun.

PINTOZZI, R. L. Modified Grocott's methenamine silver nitrate method for quick 
staining of Pneumocystis carinii  J Clin Pathol 1978 31: 803-805

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager  Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire 
Sent: Wednesday, 15 February 2012 6:22 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Hey histo gurus:
Just a little twist for you. We are planning on doing GMS and PASD on frozen 
skin sections. Is there any change in staining protocol for paraffin vs frozen. 
We plan on fixing in ETOH. I have a co-worker saying that we don't need to use 
diastase on the PASD because there are already active enzymes in the tissue 
as it is fresh. He also said that we don't need to heat the silver for the GMS. 
Not sure why. These changes don't seem right to me. But I have been wrong 
before (Yes, I admit it). Thanks for all the wisdom out there floating in the 
electrons. We are staining for fungus BTW.
Claire

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RE: [Histonet] (no subject)

[McMahon, Loralee A]

I have tried without much success.  Leica (novacastra) has an antibody.  
I have been told that it is not the antibody that is bad that it is the tissue 
that doesn't always show positivity.
We tested many biopsies that were serum positive for HepC but the immuno was 
negative. 

If you get anything to work.  Please let me know.

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Freeman, Carol 
[carol.free...@utoledo.edu]
Sent: Thursday, December 15, 2011 10:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Good Morning HistoWorld,  I am looking for a lab that runs Hepatitis C
antibody by Immunohistochemistry.  We have autopsy tissue we would like
run, so research use only or ASR is fine.  Any leads would be greatly
appreciated.  The only lab I found was in Pakistan and I was hoping to
keep it a bit more local if possible and I really don't want to have to
order and validate the antibody for one test.

Carol E. Freeman HTL (ASCP) B.S.
Department of Pathology
University of Toledo Medical Center
3000 Arlington Avenue
Toledo, OH 43614-5807
carol.free...@utoledo.edu
(419)383-5639

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RE: [Histonet] (no subject)

[Rathborne, Toni]

We switched from intercoms to phones some years back. The phone is put on 
speaker mode in the OR. I suppose problems can occur with the phone system too, 
but we haven't experienced this.
Regarding the CAP part of the question, I would call them. Ask if you can send 
a written diagnosis, which you are to receive back with the 
physician's/designee's signature. You can then document the intercom problems, 
and how you worked around it.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
jthaw...@shorememorial.org
Sent: Wednesday, November 30, 2011 10:20 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)


Hello All,

We are having a issue with our intercom system in the OR so the pathologist are 
having issues communicating frozen section diagnosis with the surgeon.
Does anyone in Histoland use a written form to send frozen section diagnosis? 
Per CAP if it is verbal the pathologist must speak directly with the surgeon.  
Any suggestions would be much appreciated.


Jennifer Thawley HT, ASCP
Histology Supervisor
Shore Memorial Hospital
(609) 653-3940


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Re: [Histonet] (no subject)

[Emily Sours]

Because your boss said to leave it at 84 degrees?


Emily

The whole point of this country is if you want to eat garbage, balloon up
to 600 pounds and die of a heart attack at 43, you can! You are free to do
so. To me, that’s beautiful.
--Ron Swanson
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RE: [Histonet] (no subject)

[Bea DeBrosse-Serra]

Do you coat the muscle biopsies with talcum powder prior to freezing? 

Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
1896 Rutherford Road
Carlsbad, CA 92008
760-603-2371



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Miha Tesar
Sent: Saturday, October 15, 2011 1:47 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Hi!
I am working in Institute of Phatology Slovenia EU. Recently I have problems 
with muscle biopsy. There are a lots of artefact that I can not explain, like 
little holes in the tissue after the tissue is being frozen. We are using the 
isopentan and the liquid nitrogen for the freezing. Can enyone give me some 
ideas haw to avoid this artefact. How important is humidity in laboratory for 
the good results?
The next problem is that after I put the tissue on the microscope slides after 
coloring the GTK all the tissue gets wrinkeld. Iam using the poly L 
lisin microscope slides or the Immuno microscope slides.
Thx in advance!
Best regards Miha from SLO

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RE: [Histonet] (no subject)

[Garrison, Becky]

I am forwarding this response from a fellow worker, D Kaylor
(The techs here have consistently good results when freezing fresh muscles
for enzyme histochemistry.

The small holes are freeze artifact.  Usually they occur when the muscle is not 
frozen fast enough.  We use methyl butane floating in a liquid nitrogen bath.  
Either way, the temp is critical.  It needs to be at least -155 degrees C.   
The muscle must be submerged and held under for about 45 seconds to one minute. 
 Do not start or dip the muscle.  Once started it must be held submerged.  We 
have never had any issues with humidity.   Are the muscle bx swimming in 
saline?   We have better results when they are submitted on saline moistened 
gauze.
We use positively charged slides to pick up our sections.  We have some 
wrinkles but that is due to the sectioning of frozen tissue not the type of 
slide.


Becky Garrison
Pathology Supervisor
Shands Jacksonville
Jacksonville, FL 32209
904-244-6237, phone
904-244-4290, fax
904-393-3194, pager
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Miha Tesar
Sent: Saturday, October 15, 2011 4:47 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Hi!
I am working in Institute of Phatology Slovenia EU. Recently I have problems 
with muscle biopsy. There are a lots of artefact that I can not explain, like 
little holes in the tissue after the tissue is being frozen. We are using the 
isopentan and the liquid nitrogen for the freezing. Can enyone give me some 
ideas haw to avoid this artefact. How important is humidity in laboratory for 
the good results?
The next problem is that after I put the tissue on the microscope slides after 
coloring the GTK all the tissue gets wrinkeld. Iam using the poly L 
lisin microscope slides or the Immuno microscope slides.
Thx in advance!
Best regards Miha from SLO

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RE: [Histonet] (no subject)

[Norton, Sally]

Hi Miha, we do many muscle bxs here as well,  the information Becky has sent 
you is exactly how we do it.  This should help you!

Sally Norton
Seattle Children's Hospital 
Seattle, WA 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Garrison, Becky
Sent: Monday, October 17, 2011 1:41 PM
To: 'Miha Tesar'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject)

I am forwarding this response from a fellow worker, D Kaylor
(The techs here have consistently good results when freezing fresh muscles
for enzyme histochemistry.

The small holes are freeze artifact.  Usually they occur when the muscle is not 
frozen fast enough.  We use methyl butane floating in a liquid nitrogen bath.  
Either way, the temp is critical.  It needs to be at least -155 degrees C.   
The muscle must be submerged and held under for about 45 seconds to one minute. 
 Do not start or dip the muscle.  Once started it must be held submerged.  We 
have never had any issues with humidity.   Are the muscle bx swimming in 
saline?   We have better results when they are submitted on saline moistened 
gauze.
We use positively charged slides to pick up our sections.  We have some 
wrinkles but that is due to the sectioning of frozen tissue not the type of 
slide.


Becky Garrison
Pathology Supervisor
Shands Jacksonville
Jacksonville, FL 32209
904-244-6237, phone
904-244-4290, fax
904-393-3194, pager
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Miha Tesar
Sent: Saturday, October 15, 2011 4:47 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Hi!
I am working in Institute of Phatology Slovenia EU. Recently I have problems 
with muscle biopsy. There are a lots of artefact that I can not explain, like 
little holes in the tissue after the tissue is being frozen. We are using the 
isopentan and the liquid nitrogen for the freezing. Can enyone give me some 
ideas haw to avoid this artefact. How important is humidity in laboratory for 
the good results?
The next problem is that after I put the tissue on the microscope slides after 
coloring the GTK all the tissue gets wrinkeld. Iam using the poly L 
lisin microscope slides or the Immuno microscope slides.
Thx in advance!
Best regards Miha from SLO

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RE: [Histonet] (no subject)

[Tony Henwood]

Miha,

Are your muscles arising in saline?
Receive them dry or in cell culture media (eg Hanks).

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager  Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Miha Tesar
Sent: Saturday, 15 October 2011 7:47 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Hi!
I am working in Institute of Phatology Slovenia EU. Recently I have problems 
with muscle biopsy. There are a lots of artefact that I can not explain, like 
little holes in the tissue after the tissue is being frozen. We are using the 
isopentan and the liquid nitrogen for the freezing. Can enyone give me some 
ideas haw to avoid this artefact. How important is humidity in laboratory for 
the good results?
The next problem is that after I put the tissue on the microscope slides after 
coloring the GTK all the tissue gets wrinkeld. Iam using the poly L 
lisin microscope slides or the Immuno microscope slides.
Thx in advance!
Best regards Miha from SLO

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RE: [Histonet] (no subject)

[CHRISTIE GOWAN]

I agree with you Jan. In my 30 + years as a histotech (yes, I am old too) I 
have seen floaters come from a variety of places but I am hard pressed to 
remember any floater coming from the water bath. Today we are blessed with DNA 
fingerprinting to determine if the floater is or is not from the patient but 
that still does not address the real issue of where did it come from and how do 
we stop it. The vendors stating that it is not an issue have never been 
re-biopsied because of a floater in with their tissue. Good discussion and long 
overdue. I look forward to the day when it is no longer an automatic response 
from all involved that it is Histology's fault. Hope you are enjoying your 
new adventures in retirement. See you next year in Canada!
 

 From: mamaw...@hotmail.com
 To: histonet@lists.utsouthwestern.edu
 Date: Wed, 5 Oct 2011 14:11:25 -1000
 Subject: [Histonet] (no subject)
 
 
 Hello everyone,After being home from the NSH for a few weeks I have been 
 pondering an issue that I think bears discussion on the histonet.There have 
 been several papers published regarding floaters and the amount determined 
 to come from traditional staining buckets. There was also a poster presented 
 at the NSH this year on the subject.When I approached several vendors of HE 
 stainers about this issue. The answers were surprisingly pretty much the 
 same. It is not an issue! Now I understand how one company can make this 
 claim as their stainer uses fresh stain on each slide. The explanations from 
 the other companies were insulting and just plain did not make sense to me. I 
 was told by a Histo tech vendor that All Histo techs know that floaters come 
 from the water bath. Well, she was talking to a histo tech and I know for a 
 fact that floaters come from a variety of places. I have seen them from the 
 doctor's office or procedure room to the stainer and every step in between. 
 Sometimes if the floater is in the block it is very difficult to determine 
 where it originated. We can however eliminate the water bath and stainer as 
 the origin in these cases. One company told me that the design of the 
 solution bottle eliminated floaters because floaters float and their stainer 
 draws solutions from the bottom of the bottle. I have probably changed 
 thousands of staining dishes during my 40+ year career (yes, I am old) and I 
 have seen lots of little pieces of tissue at the bottom of the staining 
 dishes. So, no, not all floaters float. I would love to hear feedback from 
 others on this. I guess I would appreciate feedback about the floater issue 
 as well as how a few vendors can make such claims and expect Histology techs 
 to buy it. I really felt that a few comments were insulting to our profession 
 and to the knowledge and expertise we possess. JanOmaha 
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RE: [Histonet] (no subject)

[Cynthia Robinson]

It is a good thing this vendor does not work in a histo lab because with that 
comment/attitude he/she would not last long. I have heard the water bath theory 
but upon investigation this has never been the source because every histotech I 
work with cleans it each and every time. We have traced floaters back to 
grossing stations, processors...especially placenta getting 'snagged' on a 
piece of bone...embedding forceps (we now use ones with no grooves), forcep 
warmers, stains and coverslippers. We have significantly reduced the number we 
see since we make the effort to track each one found as an ongoing QA project, 
but we still see the occasional floater.  We did find that we have to put 
tissue types which fragment easily into mesh cassettes or bags but this can 
cause issues with fluid exchange and carryover during processing. It is a 
balancing act. I have always wondering about the newer processors with 
orientation cassettes which are embed and then cut without opening. Do they see 
less floaters with this type of 'closed' system? 



Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robin...@mercyhealth.com


 CHRISTIE GOWAN christiego...@msn.com 10/6/2011 7:44 AM 

I agree with you Jan. In my 30 + years as a histotech (yes, I am old too) I 
have seen floaters come from a variety of places but I am hard pressed to 
remember any floater coming from the water bath. Today we are blessed with DNA 
fingerprinting to determine if the floater is or is not from the patient but 
that still does not address the real issue of where did it come from and how do 
we stop it. The vendors stating that it is not an issue have never been 
re-biopsied because of a floater in with their tissue. Good discussion and long 
overdue. I look forward to the day when it is no longer an automatic response 
from all involved that it is Histology's fault. Hope you are enjoying your 
new adventures in retirement. See you next year in Canada!
 

 From: mamaw...@hotmail.com 
 To: histonet@lists.utsouthwestern.edu 
 Date: Wed, 5 Oct 2011 14:11:25 -1000
 Subject: [Histonet] (no subject)
 
 
 Hello everyone,After being home from the NSH for a few weeks I have been 
 pondering an issue that I think bears discussion on the histonet.There have 
 been several papers published regarding floaters and the amount determined 
 to come from traditional staining buckets. There was also a poster presented 
 at the NSH this year on the subject.When I approached several vendors of HE 
 stainers about this issue. The answers were surprisingly pretty much the 
 same. It is not an issue! Now I understand how one company can make this 
 claim as their stainer uses fresh stain on each slide. The explanations from 
 the other companies were insulting and just plain did not make sense to me. I 
 was told by a Histo tech vendor that All Histo techs know that floaters come 
 from the water bath. Well, she was talking to a histo tech and I know for a 
 fact that floaters come from a variety of places. I have seen them from the 
 doctor's office or procedure room to the stainer and every step in between. 
 Sometimes if the floater is in the block it is very difficult to determine 
 where it originated. We can however eliminate the water bath and stainer as 
 the origin in these cases. One company told me that the design of the 
 solution bottle eliminated floaters because floaters float and their stainer 
 draws solutions from the bottom of the bottle. I have probably changed 
 thousands of staining dishes during my 40+ year career (yes, I am old) and I 
 have seen lots of little pieces of tissue at the bottom of the staining 
 dishes. So, no, not all floaters float. I would love to hear feedback from 
 others on this. I guess I would appreciate feedback about the floater issue 
 as well as how a few vendors can make such claims and expect Histology techs 
 to buy it. I really felt that a few comments were insulting to our profession 
 and to the knowledge and expertise we possess. JanOmaha 
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Re: [Histonet] (no subject)

[Angela Bitting]

I too spoke to one vendor who claimed that the floaters are not an issue and 
that their bottles drew below the level of the floating floaters. I didn't 
feel insulted, but did feel that this salesperson obviously had no actual lab 
experience or they never would have made such a ridiculous claim. 
I always put  the opinions and experiences of other Histotechs far ahead of 
anything I hear from sales reps.  
 
Angela Bitting, HT(ASCP), QIHC
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
 

 Janice Mahoney mamaw...@hotmail.com 10/5/2011 8:11 PM 

Hello everyone,After being home from the NSH for a few weeks I have been 
pondering an issue that I think bears discussion on the histonet.There have 
been several papers published regarding floaters and the amount determined to 
come from traditional staining buckets.  There was also a poster presented at 
the NSH this year on the subject.When I approached several vendors of HE 
stainers about this issue.  The answers were surprisingly pretty much the same. 
  It is not an issue!  Now I understand how one company can make this claim as 
their stainer uses fresh stain on each slide.  The explanations from the other 
companies were insulting and just plain did not make sense to me.  I was told 
by a Histo tech vendor that All Histo techs know that floaters come from the 
water bath.  Well, she was talking to a histo tech and I know for a fact that 
floaters come from a variety of places.  I have seen them from the doctor's 
office or procedure room to the stainer and every step in between.  Sometimes 
if the floater is in the block it is very difficult to determine where it 
originated.  We can however eliminate the water bath and stainer as the origin 
in these cases.  One company told me that the design of the solution bottle 
eliminated floaters because floaters float and their stainer draws solutions 
from the bottom of the bottle.  I have probably changed thousands of staining 
dishes during my 40+ year career (yes, I am old) and I have seen lots of little 
pieces of tissue at the bottom of the staining dishes.  So, no, not all 
floaters float.  I would love to hear feedback from others on this.  I guess I 
would appreciate feedback about the floater issue as well as how a few vendors 
can make such claims and expect Histology techs to buy it.  I really felt that 
a few comments were insulting to our profession and to the knowledge and 
expertise we possess. JanOmaha  
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IMPORTANT WARNING: The information in this message (and the documents attached 
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solely for the addressee. Access to this message by anyone else is 
unauthorized. If you are not the intended recipient, any disclosure, copying, 
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prohibited and may be unlawful. If you have received this message in error, 
please delete all electronic copies of this message (and the documents attached 
to it, if any), destroy any hard copies you may have created and notify me 
immediately by replying to this email. Thank you.BEGIN:VCARD
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X-GWTYPE:USER
FN:Bitting, Angela
TEL;WORK:570-271-6844
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RE: [Histonet] (no subject)

[Janice Mahoney]

Very good question Cindi.Jan

 Date: Thu, 6 Oct 2011 09:26:18 -0400
 From: robin...@mercyhealth.com
 To: mamaw...@hotmail.com; histonet@lists.utsouthwestern.edu; 
 christiego...@msn.com
 Subject: RE: [Histonet] (no subject)
 
 It is a good thing this vendor does not work in a histo lab because with that 
 comment/attitude he/she would not last long. I have heard the water bath 
 theory but upon investigation this has never been the source because every 
 histotech I work with cleans it each and every time. We have traced floaters 
 back to grossing stations, processors...especially placenta getting 'snagged' 
 on a piece of bone...embedding forceps (we now use ones with no grooves), 
 forcep warmers, stains and coverslippers. We have significantly reduced the 
 number we see since we make the effort to track each one found as an ongoing 
 QA project, but we still see the occasional floater.  We did find that we 
 have to put tissue types which fragment easily into mesh cassettes or bags 
 but this can cause issues with fluid exchange and carryover during 
 processing. It is a balancing act. I have always wondering about the newer 
 processors with orientation cassettes which are embed and then cut without 
 opening. Do they see less floaters with this type of 'closed' system? 
 
 
 
 Cindi Robinson HT(ASCP)
 Mercy Medical Center
 Dunes Medical Laboratories
 350 W Anchor Dr
 Dakota Dunes SD 57049
 phone-712-279-2768
 robin...@mercyhealth.com
 
 
  CHRISTIE GOWAN christiego...@msn.com 10/6/2011 7:44 AM 
 
 I agree with you Jan. In my 30 + years as a histotech (yes, I am old too) I 
 have seen floaters come from a variety of places but I am hard pressed to 
 remember any floater coming from the water bath. Today we are blessed with 
 DNA fingerprinting to determine if the floater is or is not from the patient 
 but that still does not address the real issue of where did it come from and 
 how do we stop it. The vendors stating that it is not an issue have never 
 been re-biopsied because of a floater in with their tissue. Good discussion 
 and long overdue. I look forward to the day when it is no longer an automatic 
 response from all involved that it is Histology's fault. Hope you are 
 enjoying your new adventures in retirement. See you next year in Canada!
  
 
  From: mamaw...@hotmail.com 
  To: histonet@lists.utsouthwestern.edu 
  Date: Wed, 5 Oct 2011 14:11:25 -1000
  Subject: [Histonet] (no subject)
  
  
  Hello everyone,After being home from the NSH for a few weeks I have been 
  pondering an issue that I think bears discussion on the histonet.There have 
  been several papers published regarding floaters and the amount 
  determined to come from traditional staining buckets. There was also a 
  poster presented at the NSH this year on the subject.When I approached 
  several vendors of HE stainers about this issue. The answers were 
  surprisingly pretty much the same. It is not an issue! Now I understand how 
  one company can make this claim as their stainer uses fresh stain on each 
  slide. The explanations from the other companies were insulting and just 
  plain did not make sense to me. I was told by a Histo tech vendor that All 
  Histo techs know that floaters come from the water bath. Well, she was 
  talking to a histo tech and I know for a fact that floaters come from a 
  variety of places. I have seen them from the doctor's office or procedure 
  room to the stainer and every step in between. Sometimes if the floater 
  is in the block it is very difficult to determine where it originated. We 
  can however eliminate the water bath and stainer as the origin in these 
  cases. One company told me that the design of the solution bottle 
  eliminated floaters because floaters float and their stainer draws 
  solutions from the bottom of the bottle. I have probably changed thousands 
  of staining dishes during my 40+ year career (yes, I am old) and I have 
  seen lots of little pieces of tissue at the bottom of the staining dishes. 
  So, no, not all floaters float. I would love to hear feedback from others 
  on this. I guess I would appreciate feedback about the floater issue as 
  well as how a few vendors can make such claims and expect Histology techs 
  to buy it. I really felt that a few comments were insulting to our 
  profession and to the knowledge and expertise we possess. JanOmaha 
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Re: [Histonet] (no subject)

[Rene J Buesa]

That will depend on the size of the fish. If less than 5 mm you could use what 
I used doing DIF of skin cases:
Place the chuck on the freezing bar and add 2-3 drops of distilled water. 
Introduce the fish inside the drop of water close to the chuck surface, and add 
another drop. Using the coolant aerosol make sure that the drop is completely 
frozen. Proceed to cut.
René J.

--- On Tue, 9/6/11, Bruijntjes, J.P. (Joost) 
joost.bruijnt...@tno.triskelion.nl wrote:


From: Bruijntjes, J.P. (Joost) joost.bruijnt...@tno.triskelion.nl
Subject: [Histonet] (no subject)
To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu
Date: Tuesday, September 6, 2011, 5:54 AM


Hi all

I have to prepare frozen slides of zebra fish, but it is not permitted to use 
OCT because of the presence of different polymers in the OCT compound. Is 
anyone of you familiar with the preparation of frozen slides without using OCT?

Thanks

Joost Bruijntjes
Zeist
Holland

TNO.NLhttp://www.tno.nl/

Joost Bruijntjes

T +31 88 866 17 38
F +31 30 694 49 86
E joost.bruijnt...@tno.triskelion.nlmailto:joost.bruijnt...@tno.triskelion.nl

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