when I go to 6 it still does it. Any ideas?
>
> Ken
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gt; Tasha Campbell, B.S.,HTL(ASCP)
> Frederick Gastroenterology Associates
> 310 W. 9th St.
> Frederick, MD 21701
> 301-695-6800 ext. 144
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Oops, yes, I am mixing up neutral red - and the recipe from Bryan is what I
use - it last forever.
I have had luck with nuclear fast red from vector - comes made up. That
lasts a little longer than lab made.
mills
ᐧ
On Wed, Aug 8, 2018 at 12:52 PM Caroline Miller wrote:
> HI Tasha,
>
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ey are great to work with!
>
> Let me know if you have any questions about them.
>
> Best regards,
> Adrienne Anderson
>
> On Monday, April 2, 2018, 2:25:46 PM MDT, Caroline Miller via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
>
>
> I am very in
Any unauthorized
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Any more info re Ultraclone's history would be most appreciated.
> >
> > Curiously
> >
> > Carl
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Thank you for all the suggestions Histonet, you wonders of knowledge!
Happy to share if anyone wants a collated list, let me know
Happy Friday!
mills
On Thu, Apr 20, 2017 at 12:13 PM, Caroline Miller wrote:
> Hi Histonet!
>
> I am on the search for a good CD8 antibody, I ha
antibodies too as they often have cross reactivity.
Any other nuances in the staining that people have found would also be
great.
thanks folks!
mills
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Yes, totally +1 to Rene, they should be fine.
(That has totally happened to me too)!
Caroline Miller (mills)
Director of Histology
3Scan, Inc
415-2187297
> On Apr 19, 2017, at 6:41 AM, Rene J Buesa via Histonet
> wrote:
>
> In 100% EthOL the tissues are completely "salv
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hip to Address:
>
> Premier Laboratory, LLC
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
>
>
> -Original Message-
> From: Caroline Miller via Histonet [mailto:histonet@lists.
> utsouthwestern.edu]
> Sent: Tuesday, April 11, 2017 11:59 AM
> To: Paula Keene
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system
> for years.
>
>
>
>
> Paula Keene Pierce, BS, HTL(ASCP)HT
> President
> Excalibur Pathology, Inc.
> 5830 N Blue Lake Drive
> Norman, OK 73069
> PH 405-759-3953 <(405)%20759-3953>
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t; >
> >
> > -Original Message-
> > From: Rene J Buesa via Histonet [mailto:histonet@lists.
> utsouthwestern.edu]
> > Sent: Thursday, February 09, 2017 10:51 AM
> > To: Jennifer ; histonet@lists.utsouthwestern.edu
> > Subject: Re: [Histonet] hand
t; help, that would be much appreciated!"
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ZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPP
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ager
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ay we want to retrim fixed samples after they
> have been in ethanol 70% for 5 months… would you trust them for IHC? HE?).
>
> Thanks again for your help
>
> Julio
>
>
>
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59-1"
> >
> > Hi,
> >
> >
> > For the life of me I can't locate a recipe for buffered zn-formalin, nor
> > online or off.
> >
> >
> > Could someone please help us out?
> >
> >
> > Thanks,
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outer edges of the specimen. Any Ideas?
>
>
> Thanks
>
> Gary
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T Southwestern
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> Medical Center
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>
> The future of medicine, today.
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dvance for your advice!
mills
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onet mailing list
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ttp://www-ncbi-nlm-nih-gov.ucsf.idm.oclc.org/pubmed/?term=Ruifrok%20AC%5BAuthor%5D&cauthor=true&cauthor_uid=11531144>
1, Johnston DA
<http://www-ncbi-nlm-nih-gov.ucsf.idm.oclc.org/pubmed/?term=Johnston%20DA%5BAuthor%5D&cauthor=true&cauthor_uid=11531144>
.
Does anyone have a copy please?
511
> Westchester, Il 60154
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accurately analyse RNA in formalin fixed, paraffin embedded (and even maybe
resin) tissues.
Asking a lot - yes, I know! However there are a lot of bright folks out
there that might just have an answer for me!
Please PM me!
yours,
mills
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dinator/ Mohs
>
> Doctors Pathology Services, Dover DE
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stalized resin (i was not responsible for
the fuzziness, but I work with what I got)!
Thanks in advance for all your wonderful advice!
yours,
mills
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piration time is for home-made
> paraformaldehyde?
>
> Joost
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sed by the processing with
> dehydration and defatting. Formaldehyde renders the tissue harder but not
> strictly smaller.
> What is the opinion of the community?
> Gudrun
>
>
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Got it! Thanks Histonet!! :)
mills
On Thu, Jan 14, 2016 at 2:59 PM, Caroline Miller wrote:
> Hi All, Does anyone have access to this paper:
> Toluidine Blue-Alizarin Red S Staining of Cartilage and Bone in
> Whole-Mount Skeletons in Vitro
>
>
> Stain Technology
Thanks in advance, I hope you are all doing well!
yours,
mills
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+1 to Rene and Anne, these posts have nothing to do with histology and I wish
they would go away.
Dr Raff, please go post them somewhere more relevant.
Yours,
mills
Caroline Miller (mills)
Director of Histology
3Scan, Inc
415-2187297
> On Dec 21, 2015, at 6:18 AM, Rene J Buesa via Histo
I tend to leave my squames on the slide, so I always wear gloves too
mills
Caroline Miller (mills)
Director of Histology
3Scan, Inc
415-2187297
> On Nov 27, 2015, at 5:50 AM, White, Lisa M. via Histonet
> wrote:
>
> I am not aware of any regulation to use glove
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tonet!
yours,
mills
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It sounds totally plausible, but just to put a spanner in the works; I have a
plastic pipette in my DPX that I leave in there for at least 6 months and I
don't get that artifact. We may have different pipettess though.
Yours,
mills
Caroline Miller (mills)
Director of Histology
3Scan, In
I mounted something!
Yours,
mills
Caroline Miller (mills)
Director of Histology
3Scan, Inc
415-2187297
> On Jul 10, 2015, at 10:55 PM, John Kiernan wrote:
>
> DPX is a polystyrene mounting medium. In principle you can make your own from
> published recipes. In practice, everyone bu
Hi Histonet!
Happy 'almost' Friday :)
Can I ask if anyone out there has ever put phantoms or other fiduciary
markings into their paraffin blocks to help with registering serial
sectioned images or other downstream processes?
thanks in advance!
yours,
mills
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n lab is a case in point
Happy hump day everyone 😄
mills
Caroline Miller (mills)
Director of Histology
3Scan, Inc
415-2187297
> On Jun 3, 2015, at 8:42 AM, Paula Pierce
> wrote:
>
> Ugh. TOO MANY REGULATIONS!
> What about plants and flowers taken to patient's rooms as
a demo
too.
thanks all!
mills
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+1 to Linda, but I have found no difference on overnight vs multiple days.
Fatty liver is hard to do on all counts! It is tough enough sometimes to get a
decent section on the slide.
Thanks for the other suggestions, certainly something I would try in the future
Yours
Caroline
Caroline Miller
pubmed searches.
thanks, in advance, for any links you can point me to,
yours,
Caroline
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er at that institution
and I can't find them. Or the other person who posted on Sihler to histonet
in 2005 - *Maria Mejia* maria <@t> ski.org. Their email address bounces
now
Is there anyone out there with first hand knowledge I can have a chat with?
Thanks all,
mills aka Caroline :)
gt;
> >
> > [cid:image001.jpg@01CF8F87.A0BD4830]
> >
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e tissue and I have
been destaining for a while. I am thinking Mayer's may be better for its
progressive nature, but any advice will be gratefully taken!
Thank you in advance for your fantastic advice!
yours,
Caroline
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tissue morphology being optimal. Any help will
> be greatly appreciated.
> Martha Davenport 859-257-1822
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sly since it first saw light.
> List manager, please remove the offending subscriber.
>
> Mike Andrews, W5EGO
> Tired old Sysadmin
> gmi...@mikea.ath.cx
>
> _
> From: Caroline Miller
> Sent: Saturday, February 7, 2015 9:04 AM
>
I think it is an anti-spam message. Asking you to add yourself to their list of
'safe' addresses.
Is anyone on this list able to ask them to try to make it stop?
Thanks!
mills
> On Feb 7, 2015, at 6:43 AM, Michelle wrote:
>
> It seems every time I reply to a histonet email, it is followed u
Yes, exactly what Mike and Geoff said. All mouse tissue, especially liver, can
be really dry and needs a 'soak'. I have left them for an hour before now but
don't leave it for longer than 4 hours though because it can start to swell and
de-process!
You will still only get a few non-chattery sec
When I worked in a research core (which was only last week, but I just changed
jobs). I have processed and cut (with varying degrees of success) fake meat
samples for a company. They were mainly made of grains and mushed veggies.
The problem was the samples were not consistent and there was no
:)
Thank you all for your great posts, I enjoy them immensely, especially Peggy's
wisdom (thanks Lee for sticking around a little while, and thanks for Peggy)!
Happy Friday to you all!
Caroline
Caroline Miller
Director, Histology and Light Microscopy Core
Gladstone Insti
I use prolong gold from life tech (formerly molecular probes and now part of
the world dominating thermofisher) without Dapi as I do that separately, for IF
and aquaslip from American master tech for non IF aqueous mounting
Good luck!
Caroline
Caroline Miller
Gladstone Institutes
Same here...
Caroline Miller
Gladstone Institutes
www.gladstoneinstitutes.org
Tel: 415 7342566
Cell: 415 2187297
> On Nov 12, 2013, at 5:31 PM, Patsy Ruegg wrote:
>
> I have done picrosirius red for years and never used phosphomolybdic acid so
> it would be hard to s
However, I use the Shandon sequenza, and I still see edge effect. I always
thought it was a fixation related. Maybe even that the edge staining is 'more
real' than the rest!!
Histology, it's all voodoo!!
Caroline
Caroline Miller
Gladstone Institutes
www.gladstoneinstitute
going
on completely empirical evidence!
Yours
Caroline
Caroline Miller
Gladstone Institutes
www.gladstoneinstitutes.org
Tel: 415 7342566
Cell: 415 2187297
On Sep 18, 2013, at 7:21 AM, Rene J Buesa wrote:
> Why do you have to store fixed specimens for that long? If you are going to
&
from changing the
Machine
Caroline Miller
Gladstone Institutes
www.gladstoneinstitutes.org
Tel: 415 7342566
Cell: 415 2187297
On Mar 6, 2013, at 7:33 AM, Bob Richmond wrote:
> Helayne Parker is concerned about a woman 5 weeks pregnant working in
> an ordinary histopathology lab.
>
>
(Sorry hit send too soon, see below)
Caroline Miller
Gladstone Institutes
www.gladstoneinstitutes.org
Tel: 415 7342566
Cell: 415 2187297
On Mar 6, 2013, at 7:50 AM, Caroline Miller wrote:
> I was recently pregnant (I have a happy and healthy 8month old). I work in a
> research lab a
WellI am 6 months pregnant, and was planning on taking a pot of formalin
and a few blades into the delivery suite to get some of my placenta...I am
in real need of a good CD31 and CD34 control!
We all give our bodies to this - right ;P
Caroline
Caroline Miller, M.Sc, DIC, AIBMS
he histoland: Is this all true? Are there any sticking
points we may run into with the regulations? How could we phrase the
contract so that there is no chance of come-back? Big questions, I
know, but any advice would be gratefully received
Yours
Caroline
Caroline Miller, M.Sc, DIC, AIBMS
Ma
1% cHCl in 70% ethanol (reagent alcohol), couple of seconds is plenty
Caroline
Caroline Miller M.Sc DIC AIBMS
Co-manager
Gladstone Microscopy and Histology Core
J David Gladstone institute
1650 Owens Street
San Francisco
CA
94158
www.gladstone.ucsf.edu
On Jun 19, 2009, at 17:01, thisis
Yes please!!! I am desperate for a new microtome!!!
Caroline
Caroline Miller M.Sc DIC AIBMS
Co-manager
Gladstone Microscopy and Histology Core
J David Gladstone institute
1650 Owens Street
San Francisco
CA
94158
www.gladstone.ucsf.edu
On Mar 31, 2009, at 6:29, "Cathy Mayton" wrot
do I have to do to be qualified in the
USA??
Thanks
Caroline
Caroline Miller
Co-Manager
J David Gladstone Institutes Histology and Microscopy Core
1650 Owens St
San Francisco
CA 94158
Tel: 415 734 2566
Fax: 415 355 0824
http://www.gladstone.ucsf.edu/gladstone/site/histology/
und!
Caroline
Caroline Miller
Co-Manager
J David Gladstone Institutes Histology and Microscopy Core
1650 Owens St
San Francisco
CA 94158
http://www.gladstone.ucsf.edu/gladstone/site/histology/
cmil...@gladstone.ucsf.edu
On Feb 7, 2009, at 1:11 PM, Lee & Peggy Wenk wrote:
95-100% also
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