[Histonet] anti-mouse NK marker

Hi, Does anyone have a good anti-mouse NK cell marker for frozen mouse tissues? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo

[Histonet] histology tracking systems for pharma or contract lab

, necropsy timepoints, etc). I am interesting in hearing from people in other pharma/biotech/CROs to see what they are using to capture this information. Thanks, Kim   Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet

[Histonet] alternates to cryojane

Hello eveyone, Are there any other tape transfer systems for the cryostat other than the cryojane? Thanks, Kim   Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu

[Histonet] eosin in the processor

guessing no, but I want to be sure). Thanks, Kim   Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] CD31 for FFPE IHC on Mouse

I second the Dianova antibody - we have had very good luck with it.  Standard citrate HIER works for us.   Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Patsy Ruegg pru...@ihctech.net To: 'Mark Elliott' mark.elli...@hli.ubc.ca; histonet

[Histonet] human vimentin in mouse tissue

  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA  ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] endogenous peroxidase in eosinophils

Anyone have tips for quenching endogenous peroxidase in eosinophils?  Our standard px block is not doing the job (Biocare Peroxidazed-1). Thanks, Kim   Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet

[Histonet] fibrin IHC on FFPE mouse tissue

Is there an antibody out there that will stain for fibrin on FFPE mouse tissues; been searching but haven't found one.  Does everyone just do a special stain when looking for fibrin?   Kim     Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet

Re: [Histonet] Hydrating Frozen Tissues embedded in OCT for Sectioning

As long as you don't need to use them for RNA analysis, the easiest thing to do is just rub your finger (without glove) across the block (very briefly) and then take the section.  This will probably do the trick and hydrate it just enough to make a difference.   Kim Kim Merriam, MA, HT(ASCP

Re: [Histonet] Hydrating Frozen Tissues embedded in OCT for Sectioning

Also, make sure that the block sits in the cryostat for a while to acclimate to the temperature.  The cutting temp for lung (correct me if I'm wrong here) is probably about -20; the block should be the same temperature as the cryostat. Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA

Re: [Histonet] Cytology CSF Cell Pellets made from Histogel

Good luck! Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Turner, Leandra lturn...@seton.org To: histonet@lists.utsouthwestern.edu Sent: Tuesday, February 21, 2012 12:26 PM Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel Hello

Re: [Histonet] Cytology CSF Cell Pellets made from Histogel

I forgot to mention, we prepare these specifically for IHC; so yes, you can do IHC on them. Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Kim Merriam kmerriam2...@yahoo.com To: Turner, Leandra lturn...@seton.org; histonet@lists.utsouthwestern.edu

Re: [Histonet] Immunofluorescence staining/minimizing background staining

is it fairly clean (ie - very little autofluorescence in that channel) Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Kasai, Miki (NIH/NCI) [E] kas...@mail.nih.gov To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Monday, January 30, 2012 4

Re: [Histonet] RE: Interview Questions

several stories related to this question (especially in research, when you have PIs collecting tissue for you that often have no clue what they are doing)!!!     Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA         From: Burton, Lynn lynn.bur...@illinois.gov

[Histonet] neutrophil marker for FFPE mouse tissue

Hello! What antibodies are people using to stain for neutrophils in FFPE mouse tissue? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo

Re: [Histonet] Efficacity of fluorochrome in Immunofluorescence

/Secondary #2 9 NoPrimary (diluentonly)/Secondary #1 10 No Primary(diluentonly)/Secondary #2 11 No Primary (diluentonly)/Secondary #1/Secondary #2 12 Diluent only (or nothing) 13 DAPI only   Best of luck! Kim, Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From

[Histonet] proximity of proteins for co-localization via IF staining

nM of each other).   Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] brain sections cut on Cryo Jane apparatus are falling off slides during ethanol series for a cresyl stain

I was having similar issues with frozen rat and human cartilage with the cryojane.  After a lot of trial and error; I have found that the 4X slides and air-drying overnight has worked extremely well for us.  The tissues never fall off any more!   Good luck, Kim Kim Merriam, MA, HT(ASCP)QIHC

[Histonet] counterstain for fast red

Hi Everyone,   We are currently running some alk phos staining protocols with a fast red chromogen.  My pathologist would like to use something other than Hematoxylin as a counterstain.  What does everyone recommend?    Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA

Re: [Histonet] ACD RNAscope

with their kit on frozen sections.   I have shipped them some of my slides and they have agreed to test them in their lab.   Let me know if you have any success! Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Louise Renton louise.ren...@gmail.com To: Histonet Histonet@lists.utsouthwestern.edu

Re: [Histonet] instructions for steaming slides

Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Kant, H.J.G. van de (Henk) h.j.g.vandek...@uu.nl To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Sent: Thursday, August 25, 2011 1:49 AM Subject: [Histonet] instructions for steaming slides Dear All, Can somebody help

[Histonet] hospitality events at NSH

Anyone heard anything about hospitality events at the NSH? Kim   Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Reorienting frozen tissue

the old OCT to melt a little bit with the new OCT, that way you will have a block without any seams in it.   Good luck! Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Daniela Bodemer daniela.bode...@mcri.edu.au To: histonet@lists.utsouthwestern.edu Sent: Tuesday, August 2, 2011 7:44 PM Subject

Re: [Histonet] Mouse vs. Human cells

Years ago, we used to distinguish human fibroblasts in mouse tissue with vimentin; which stains human cells quite nicely, but does not cross to mouse.   Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Carl Postenka cposte...@hotmail.com To: sdys...@mirnarx.com; histonet

[Histonet] RNAscope ISH technology

Happy Friday!   Does anyone have experience using RNAscope ISH technology on FFPE tissues? http://www.acdbio.com/whats_rnascope.html   Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] molecular path email listserv

Hi Everyone,   Does anyone know of an email listserv similar to this one for molecular pathology?   Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu

Re: [Histonet] Biocare Intelipath

the Intellipath instrument a few years ago.  They are by far one of the best companies I have ever worked with, they are super customer-oriented and the service engineers come quickly, if your machine needs to be fixed. I would defintely get a demo into your lab to see if it fits your needs. Kim

[Histonet] Senior Histologist/Lab Manager position at AstraZeneca in Waltham, MA

Hello all,   Below is a great job opportunity for a senior histologist/lab manager at AstraZeneca in Waltham, MA.  I am posting this for a colleague, so please do not email me if you are interested.   Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA   http://jobs.astrazeneca.com/jobs/39

[Histonet] TRAP staining kit

Hi All,   Does anyone know if there is a TRAP staining kit available for histology (and who sells it)?  I have found a bunch of them on google, but they are all for cell culture.   Thanks, Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA

[Histonet] OFF-TOPIC - stuff to do in New Orleans

built-in babysitters). Thanks a bunch, Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] fibroblast marker in mouse tissue

Hi All, What is everyone using to stain for fibroblasts in frozen mouse tissue?  Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] cryojane problems

Brett- I have been using the 4X slides, but they don't seem to help.  I dont have this problem with the cartilage alone, but when there is bone attached, it is a nightmare. Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Connolly, Brett M brett_conno

[Histonet] cryojane problems

on the tape.  I have tried increasing the number of flashes (to a whopping 10!), hoping that this will help adhere the sections, but it hasn't. Any tips for me? Kim   Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet

[Histonet] special stain for H. billis

Good morning everyone, Does anyone know of a special stain that is specifically for H. billis.  I don't know much about bacteria, so I am not even sure which bacterial stain would work on this.  Any comments would be appreciated. Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA

[Histonet] human on human IHC

Hi, Does anyone know of a human-on-human IHC kit similar to the mouse-on-mouse kits that are available? Thanks, Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] tungsten knives for cryostat

and get a new adaptor for my knife holder.  What company makes the best tungsten knives? Thanks, Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo

[Histonet] off-topic cryosectioning as security check word

Hi All, I thought you all would get a kick out of this.  I was ordering some tickets on ticketmaster and look at the word that popped up for the security check - it was cryosectioning! I managed to get a screenshot of it; it is attached.  We have infiltrated main-stream culture! Kim Kim

Re: [Histonet] Chilling Tray for Blocks

you are chilling them. Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Sherwood, Margaret msherw...@partners.org To: Jay Lundgren jaylundg...@gmail.com; Laurie Colbert laurie.colb...@huntingtonhospital.com Cc: histonet@lists.utsouthwestern.edu Sent: Wed

[Histonet] cryojane question

to prevent the folds or the lift-off. Thanks, Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] ISH on histogel cell pellets

Hi Everyone, We have some cells that we have processed and embedded in histogel (thanks to everyone for the protocols that were sent to me a couple of weeks ago). We are planning to do IHC and ISH on these pellets.  Will the histogel interfere with the ISH procedure at all? Kim  Kim Merriam

Re: [Histonet] Histodeck Flash Cards

and explanations on the back.  They were pretty expensive (75-80, I think), but would probably help out a lot when studying for an exam (a non-histologist could ask the questions and help you study)!  I will probably enlist my 9 year old, who gets a kick out of this kind of thing. Kim  Kim Merriam

Re: [Histonet] ICC slide storage

the wrapped slides to acclimate to RT before you use them. Happy staining! Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: sgoe...@xbiotech.com sgoe...@xbiotech.com To: histonet@lists.utsouthwestern.edu Sent: Tue, May 18, 2010 2:36:56 PM Subject

[Histonet] Vector ABC-HRP

Hi All, This may seem like a no-brainer, but can I dilute Vector's ABC-HRP solution in TBS instead of PBS?  I have always used PBS, but we are looking to avoid PBS entirely for one of my projects. Had anyone used TBS to dilute ABC-HRP? Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA

[Histonet] (no subject)

against everything I was taught in histology, but I am really at a loss here. Anyone have any hot tips for me? Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

Re: [Histonet] (no subject)

Thanks for the tips everyone!  I am going to order some agar (histogel) and use it!  We are all very excited around here about this, we have been pulling our hair out over these darn pellets.  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Cormier

Re: [Histonet] IHC and IF

you could then detect with an anti-Rabbit polymer (or whatever you normally use to detect a rabbit primary). Good luck, Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Thotakura, Anil Kumar a.thotak...@imperial.ac.uk To: Histonet@lists.utsouthwestern.edu

Re: FW: [Histonet] studying for ASCP certification exam

The NSH sells a set of study guides on histology (maybe 10 of them, on fixation, various special stain categories and the like), I think you will find them quite helpful to see how much you actually remember after reading the textbooks. Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA

[Histonet] NSH online registration

Anyone else having trouble with the online registration for the meeting in Seattle? Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo

Re: [Histonet] Incubation of Ab more then 1 hour in autostainer

on the machine. Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Margaryan, Naira nmargar...@childrensmemorial.org To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Thu, March 18, 2010 3:38:33 PM Subject: [Histonet] Incubation of Ab

Fw: [Histonet] frozen sections of cartilage

 Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA - Forwarded Message From: Kim Merriam kmerriam2...@yahoo.com To: Histonet histonet@lists.utsouthwestern.edu Sent: Mon, February 22, 2010 2:37:58 PM Subject: [Histonet] frozen sections of cartilage Hi Everyone, Any tips for keeping frozen

[Histonet] frozen sections of cartilage

Hi Everyone, Any tips for keeping frozen sections of cartilage from falling off the slides during IHC staining?  We should not have to do HIER, but they still fall of the slides quite easily. Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA

[Histonet] Re: [IHCRG] Hamamatsu Nanozoomer

I would like to thank everyone that responded to my questions about these scanners.  These answers have been very valuable to my boss and I and will be taken into account when we decide on which system to purchase!  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA

[Histonet] Fw: [IHCRG] Dianova rat anti-mouse CD31

 kit (15 minutes each) + DAB.  The path labs (for my company) that are in California and Seattle used the same retreival but different detection methods and got similar results. Kim From:ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of Kim Merriam Sent: Wednesday, January 27

[Histonet] Hamamatsu Nanozoomer

conferencing purposes).  We are interested in it because this instrument can be adapted for fluorescence (with Aperio, you need to purchase 2 separate instruments; one for light microscope and one for fluorescence). Any insights would be greatly appreciated. Thanks, Kim  Kim Merriam, MA, HT(ASCP)QIHC

[Histonet] Dianova rat anti-mouse CD31

tissues (and perhaps some additional titrations), but I thought I would pass this along to everyone. Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman

Re: [Histonet] fumehood

Surgipath makes a nice tabletop fume hood, we have one in our lab and it works quite well. http://www.surgipath.com/us-en/Products/equipment-accessories/fume-hood Good luck! Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: rmweber...@comcast.net

Re: [Histonet] requirements for certification

.  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Jennifer Anderson jander...@halozyme.com To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wed, January 13, 2010 4:50:53 PM Subject: [Histonet] requirements for certification Hello

[Histonet] opinion please

Help! My refrigerator died over the weekend.  This fridge had most of my antibodies and IHC reagents.  When I came in, the temperature inside the fridge was a balmy 37C! Do you think any of my reagents are still usable? Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA

[Histonet] CD68 on FFPE human tissue

What antibodies are people using for this marker? Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Floaters in Waterbath

I have always used pieces of phone book paper.  Just ask everyone to bring in their old phone books, and tear the papers off at the seam.  They provide just the right amount of absorption, are the perfect size, are free and a useful way to recycle! Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge

[Histonet] harvest DRG from dogs

Hi Everyone, This is slightly off-topic, but does anyone know of a textbook or website that will give information on how to harvest DRG (dorsal root ganglion) from dogs? Thanks, Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing

[Histonet] IHC on plasma cells

Hi everyone, I have been asked to do IHC on plasma cells of FFPE human tissue.  Anyone have a good antibody (and method) for this procedure? Thanks, Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet

[Histonet] histology for kids

prepared slides (wtih real stains) for them to look at. Any ideas? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] histology for kids

Thanks to everyone that emailed me, I received so many ideas!  I will let you all know what I end up donig. Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Kim Merriam kmerriam2...@yahoo.com To: Histonet histonet@lists.utsouthwestern.edu Sent

[Histonet] anyone tried to get onto the NSH website today?

Good morning everyone, I have tried several times to get onto the NSH website today and I keep getting an error.  I am wondering if it is down or if there is something wrong on my end. Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet

[Histonet] Fw: [champ] website for introducing Molecular diagnostics, proteomics etc

Hi All, I subscribe to another listserve and I thought this link might be of interest to people on the histonet (see the link below):  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA - Forwarded Message From: Deborah Payne deborah.pa...@utsouthwestern.edu To: AMP Membership Listserv

[Histonet] aggrecan IHC on articular cartilage

using a more routine retrieval?  Also, any antibodies to recommend?   Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] HRP-labeled primary antibodies

to amplify the signal. Also, do you think the final antibody concentration would need to be higher than with traditional, unlabeled primaries? Thanks, Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet

Re: [Histonet] HRP-labeled primary antibodies

I got some great ideas.  Thanks everyone! Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: anh2...@med.cornell.edu anh2...@med.cornell.edu To: Kim Merriam kmerriam2...@yahoo.com; Histonet histonet@lists.utsouthwestern.edu; ih...@googlegroups.com Sent

Re: [Histonet] Floor materials

I know they are expensive, but I think that epoxy floors are best in any kind of lab that uses solvents.  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: louise renton louise.ren...@gmail.com To: Histonet@lists.utsouthwestern.edu Sent: Wednesday, April 29

[Histonet] firefly luciferase

Hi, Is anyone doing firefly luciferase staining in FFPE mouse tissues?  Any antibody recommendations? Thanks, Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] IHC for adenoviral vectors

Hi All, I was wondering if anyone has ever done IHC to look for the presence of adenoviral vectors in tissue (ie - to track where the viral vector has spread in the body). Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list

Re: [Histonet] HT training

, preclinical contract lab and big-pharma.    Anyway, you should be able to qualify to take the exam if you work with a boarded DVM pathologist (DACVP), that is how I qualified to take both of them.   Good luck! Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA --- On Wed, 2/11/09, Jennifer Anderson

[Histonet] PhosStop and Complete and cryosections

 will occur if I use this reagent repeated during the day (it would probably only be about 8 racks of slides in the fixative) should I keep it cold (ie - fix the slides @ 4C) or can I use it at Room Temp (fix the slides at RT) Any insight would be great!   Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC

[Histonet] Complement C3 for mouse FFPE

Hi,   Can anyone recommend a good antibody for complement C3 on mouse FFPE tissue?   Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo

Re: [Histonet] training materials

The AFIP book (Laboratory Methods in Histotechnology, edited by Edna Prophet, et al) has nice chapters on specimen orientation and embedding  It is not really a training manual, but it has some nice pictures as to how certain tissues should be placed into the molds.  Kim Merriam, MA, HT(ASCP

Re: [Histonet] Primary works with ABC but not IF

.  The Perkin Elmer FITC-TSA kits also work well, but cost about 3X as much as Invitrogens. If you send more information about your staining protocols, we could probably give you more specific advice. Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From

Re: [Histonet] IHC on fresh frozen

the slides into wash buffer and continue with IHC.  I have heard that this fixative could be problematic with some antigens, but we have not found that to be the case with anything we have used in my lab.  Insulin should be fine. Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA

[Histonet] anti-mouse ferritin

Hi,   Any recommedations for anti-mouse ferritin antibodies (looking to stain FFPE mouse tissue).   Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu

[Histonet] Re: [IHCRG] HIER

to follow strick regulatory guidelines (although I try to run the lab in a GLP-like fashion). Just my 2-cents. Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Perry, Margaret [EMAIL PROTECTED] To: [EMAIL PROTECTED] [EMAIL PROTECTED]; histonet

[Histonet] conjugated anti-FITC antibody

Hello All! I was wondering if anyone was aware of an anti-FITC antibody that was conjugated to either HRP or Biotin (eg HRP-rabbit anti-FITC or Biotinylated rabbit anti-FITC).  If such a thing exists, can you tell me who sells it? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA