I agree with Carl Hobbs: "Perhaps your H2O2 ( substrate) stock has 'gone off'?
That will give you a weaker/negative DAB result."
If your frozen DAB aliquots keep the same color, I would be very suspect of the
Hydrogen Peroxide. It can become unstable very quickly.
Terri L. Braud, HT(ASCP)
HNL
I just returned from a week off to see the notice of John Caggiano's passing.
My deepest condolences to his family and friends. His unwavering commitment
and support to the field of Histotechnology can't be understated. RIP, you
will be missed.
Terri L. Braud, HT(ASCP)
HNL Laboratories for
We have something that is a bit of a hybrid that we call a "dummy" requisition.
A person "orders" a "test" for pathology or cytology in the hospital
information system. The form they fill out has all of the information that
would appear on a paper requisition. When completed, it prints to a
We've used the UV light for the hood disinfectant, as well as just taken a
break out in the sunshine. Both worked just fine
Terri L. Braud, HT(ASCP)
HNL Laboratories for
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3689
Fax: 215-938-2021
Honesty
As someone who has been a supervisor in 3 institutions for 35 years, this is
not that way to improve productivity. To log in every minute of activity feels
very punitive and I can't imagine that it would be well received by staff. The
best way to improve productivity is to start by asking
The inspector is wrong. Sometimes there is an expiration upon opening, but the
majority of reagents do not have this. Just smile and say OK like you really
mean it, then check your CLIA regulations and be prepared to back up your
defense for the next inspector.
Terri L. Braud, HT(ASCP)
HNL
I may have an answer for you. Your Prisma stainer only runs the heater when
needed. With full runs, the oven stays warm, but later stain runs allow for
the heater to cool down. When your rack goes into the dry station, the heat
comes up from the bottom to start to dry the slide, thus the
I have long used the kits from Sigma and found them to be extremely reliable.
Call their tech helpline for advice, or reach out to your pathologist for their
preference. I know that "homemade" saves money over kits, but for me, my most
costly and precious resource is my tech time. Save
We use the Tissue Tek Glas with our Ventana labeled slides with no problem.
The key is to insure the label does not hang off the edge in the slightest.
However, it does not put them in a tray, however, that function only takes
about 10 seconds.
Terri L. Braud, HT(ASCP)
HNL Laboratories for
My first instinct is to ask what detection system you are using. If you are
using an Avidin-Biotin detection, kidney is notorious for having excess biotin
that must be blocked, or better yet, use a polymer based detection system.
That needs to be answered before further trouble shooting.
Terri
I'd love to hear your input
On breast mastectomy cases with sentinel lymph nodes, what is your cutting
protocol for sentinel lymph nodes sent for frozen section?
How many frozen levels?
How many permanent section Levels?
Any unstained ffpe sections saved or stained IHC?
I'm just trying to make
We have used the IMEB band saw for years and love the results. It has a
similar footprint and will cut through anything, including a steel pin. It
also has a diamond blade and optional water cooling. It does seem a little
awkward to clean, and I wish the protective housing was a little
Aside from the terrible legal liability that you would open your institution
to by asking them to operate "dangerous" equipment when they are not in your
employ, sitting at a strange microtome/waterbath set up with different knives,
paraffins, processing, just would not be an accurate test.
I
Here our Histology techs and tech assistants collect all FNAs. We stopped
making thyroid smears 2 years ago, preferring to collect 6 passes, 5 directly
flushed into Cytolyte for CB and Thin Prep, and one pass into Affirma holding
solutions for possible testing for equivocal samples. Using
A very interesting question, and I suspect you will receive a wide range of
answers. I, too, was taught to cut the first level, then 3 unstained for
possible IHC, then second and 3rd level. However, further research has shown
that micrometastisis has little effect on patient outcomes so in
Besides the GYN reviews, we use our CAP surveys and circulate the ER/PR, Her2,
CD20, Ki-67, and MMR panel between all pathologists for a blind read. The
original survey is reported in rotation, then all other pathologists are given
blank copies of the answer sheet and asked to fill it out.
I'm not holding my breath for this one. Not to say it will never happen, but
don't look for this one anytime in the foreseeable future. Terri
Terri L. Braud, HT(ASCP)
HNL Laboratories for
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3689
Fax: 215-938-3874
We've been using our Leica IPS and IPC printers for over 15 years, first as
stand-alone units, and now, integrated into CoPath. What a workhorse these
instruments this have been! Their modern equivalent is almost identical, so
parts are readily available. My recommendation would be to repair
Just a word of caution that unless you are quite sure of the cleanliness of
your cryostat, or the infectious status of the specimens cut in your cryostat,
freezing spray is the worst product to use in a cryostat. It creates
aerosolized particles that can linger, and infect, for hours.
At our
Terri L. Braud, HT(ASCP)
HNL Laboratories for
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3689
Fax: 215-938-3874
Honesty
AccouNtability
AgiLity
CoLlaboration
CoMpassion
You can reach the person managing the list at
Richard's suggestion is the most sensible suggestion that I've seen in a while,
however, it means less money for reagent manufacturers, so I doubt we will ever
see it come to pass. - Terri
--
Message: 2
Date: Thu, 17 Mar 2022 13:45:44 +
From: "Cartun, Richard"
We average within 1 minute for needle core and small biopsies, within 15 for
lumpectomy or mastectomy.
Terri L. Braud, HT(ASCP)
HNL Laboratories for
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3689
Fax: 215-938-3874
Honesty
AccouNtability
AgiLity
After a ba-zillion years in Histology, I've found there is no substitution for
having the floors stripped every 2 weeks. The trick is NOT to apply floor wax
after stripping. We've also been currently using mats in key areas where the
most traffic is. This prevents wax from being ground into
We do not re-use.
Terri L. Braud, HT(ASCP)
HNL Laboratories for
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3689
Fax: 215-938-3874
Honesty
AccouNtability
AgiLity
CoLlaboration
CoMpassion
-Original Message-
From:
Wishing you all the best in your retirement, Tim. In reading your history in
Histology and EM, I can see how your experience has led to so many wise,
educational posts on the Histonet. As others have also expressed, I hope you
will continue to be a contributing part of the Histology world.
We use the breast reduction mammoplasty protocol, 4 blocks each breast, single
level, each block, unless the gross dissection reveals an unexpected anomaly.
Terri L. Braud, HT(ASCP)
HNL Laboratories for
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3689
Fax:
Tony - We were on the same page. We purchased fresh beef liver, sectioned and
froze it, cut and stained duplicate blocks and had the pathologist sign off on
the process. We were also able to get some tonsils delivered fresh and did
them, too. Terri
Terri L. Braud, HT(ASCP)
HNL Laboratories
Sorry, E. Wayne, but in the USA, according to December 2004 JAMA The Journal of
the American Medical Association 292(20):2500-5, recent examination of these
issues by a US federal court resulted in a ruling that individuals do not
retain rights of ownership or control of biological materials.
Our policy, and the policy at 2 other institutions that I've worked at, is a
mirror of what Richard Cartun stated. We don't release soft tissues to
anything but a funeral home.
Terri L. Braud, HT(ASCP)
HNL Laboratories for
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph:
I'm not sure I understand why you would want to replace your 1850s just because
Leica is closing the support. I have 2 and they are real workhorses. There
are 2 independent companies in the area that can repair and perform PMs. One
is Bel-Air and the other is Advantik. I'm sure there are
We have encountered a similar problem with one of our two processors is down.
Shortening the longer schedule produced underprocessed fatty tissues and over
processed biopsies, so we run the biopsies on the short schedule overnight, run
a quick clean cycle, then the larger tissues on the 10hr
First of all, freezing spray should NEVER be used in a cryostat. It produces
dangerous aerosolized pathogens that linger in the air, just waiting to infect
someone. There should be no exceptions.
As to the ice artifact problem, not surprising to see this in brain since it
is so fragile and
A. USE EYEPIECE WITH OCULAR MICROMETER ALREADY IN PLACE.
B. Place the stage micrometer on the stage and focus on some portion of the
scale with the 10X objective.
C. Looking through the microscope, examine the ruling of the stage micrometer
so that you can distinguish between
the large (0.1
CAP specifically addresses Scott's question in the checklist (see below, copied
from the Checklist)
ANP.22780
Laboratories confirm assay performance when conditions change that may affect
performance.
NOTE: A change in antibody clone requires full revalidation/verification of the
assay
As a long time supervisor, I wanted to respond to the article about handling a
terrible employee and add a few observations.
Let me state, I am very process oriented, so I wanted to make a few
observations. I try not to see a "terrible employee". To me, that adds a
personal connotation that
Everyone here LOVES our Hologic Faxitron. A self-contained unit, so simple to
operate. We can instantly x ray anything, including sometimes, the paraffin
blocks, to see the microcalcifications to be sure we get them on a slide if
they are there.
Terri L. Braud, HT(ASCP)
Anatomic Pathology
We were using mModal and are in transition to Voicebrook which uses Dragon as a
speech recognition set up with SQ CoPath for both grossing and microscopic
dictations. We have mixed user feelings, too. Our PA who does the majority of
the gross, uses it and has lots of templates preset to
I have to agree with Bob. Bouin's should not be used as an ink fixative,
especially in a spray bottle. We use 5% Vinegar, or glacial acetic acid, and
it works just fine.
Just my 2 cents - T
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
HNL Labs, Holy Redeemer Hospital
1648 Huntingdon
Interesting question - Here, as previous places I've worked, we accession
outside surgical consults with one of our routine Surgical numbers, and outside
Cytological consults with a routine Cytology accession number. If we receive
multiple mixed cases, they are all reported as a Surgical
60% of floaters from the water bath? I find that really hard to believe.
The Gephardt and Zarbo CAP study from 1996 showed reported the results of a
Q-Probes study of 275 laboratories and documented a frequency of contamination
of between 0.6% and 2.9%, depending on the study method. Their
Hello Histo Peeps - What are the clear crystals that form in Hematoxylin? We
use Richard-Allen 7211. We love the stain, but occasionally, when a bottle has
been previously opened, we find clear crystalline precipitate forming in the
bottom. Today, we had a huge one (over an 2" in greatest
I've had great personal experiences with Sunquest CoPath Plus. It is very
flexible for customization and at 3 differing institutions, has been extremely
reliable. This does, however, come at a rather expensive cost. I think that
in choosing a system, one needs to take into account
Actually, there is another route for some of us older techs. You can be
grandfathered in without a degree if you have a certain prerequisite number of
hours in specific science courses and prior documented training in grossing.
Of course, I'm so old, I don't remember the details. LOL
Terri
We have the Leica linear stainer (Also sold by Avantik?) and have it set at 7
second intervals. The stain takes about 100 seconds.
Slides are fixed in the Coplin jar with 95% Alcohol then loaded:
The stations are in order:
1. Water wash
2. Hemotoxylin (Richard Allen 7211)
3. Hemotoxylin (Richard
A quality check can be accomplished in 2 places. It can be done at cutting,
but it should already be being done and it doesn't seem to be working.
Ideally, the stained H should be checked against the block face as it is
pulled from the coverslipper to be given to the pathologist. Then it can be
Any silver stains can be lightened or removed by the old method for removing
mercury pigment. This particular timing will remove it all so I would start
the sequence of steps in 5 second increments, repeat or lengthen times as
needed.
1. Hydrate to distilled water.
2.Place in Lugols iodine for
Hi fellow Histonetters - I'm in need of some help, please
Background - We currently use agar to capture our scant cell blocks for
processing. I am unfamiliar with the Plasma/Thrombin method of cell block
preparation and am interested in comparing it to our current method
Request - Could you
Hi Val -
I'm sorry that you feel that your concerns are not being addressed. I am the
safety officer for our entire lab as well as the chair on our hospital's
Environmental Safety and Hazardous Materials Committee. I do think that Tim
has a valid point that Xylene is detectable by smell long
Dear Dorianne -
Over 40+ years, I've seen all sorts of attempts at solving the "slippery floor"
issue; mats, special flooring, etc. In my experience, there is no substitute
for having the Environmental Services folks, strip the floors every 2 weeks. I
find that while applications of floor
We use the same protocol for all H
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal
Sent: Friday, January 03, 2020 1:00 PM
Subject: Histonet Digest, Vol
We do it all the time.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal
-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu
Hello Histopeeps -
I need your help, please. I need to improve our processing of Descemet's
membrane, more specifically keeping track of that little wisp of clear
colorless tissue? Do you have any tricks to help keep track of it through
processing? Inks? Embedding gel? Anything?
I'm open
thing to always
consider is cap/Asco time fixation guidelines for breast biopsies. Having a
weekend run gives you more flexibility.
G
Sent from my iPhone
> On Dec 5, 2019, at 1:40 PM, Terri Braud via Histonet
> wrote:
>
> In my experience in 2 small hospital labs, weekend cove
In my experience in 2 small hospital labs, weekend coverage is just not worth
it. If it is a major surgery, then the patient will be recuperating and if it
is just a biopsy, the ordering physician is rarely available to correlate the
Pathology findings with the clinical findings. Most tell
The report must be amended, not deleted. The amended reason should state the
reason for the amendment, and the correction. "This report is amended to
correct the patient data to the correct patient"
All electronic reports that are downstream must show that correction.
You can never just delete
We collect our FNAs in CytoLyt. We also use it to wash all our non-gyn fluids,
but then we fix the cell block "pellet" in formalin.
We have had no problems with immunos, and are able to lyse the RBCs to provide
a nice, clear specimen.
Hope this helps.
Terri L. Braud, HT(ASCP)
Anatomic
1. Extremity carrier (Olszewski, Dawn)
I completely agree with Karen Bowden's response,
"I work in a research lab so this may not go over well.? How about using
a cart and a child size body bag? You would not be able to see through
the bag and there shouldn't be any smell and you most likely
Our Histology Techs also assist with Bone Marrows in CT, though there is little
to do. We collect the aspirate in Heparin Tubes and the core in Bouins. We
bring it back to the lab and make our smears from one of the heparin tubes.
The other tubes go for special studies. I'd like to think
My question is why would you ever want to use an aerosol in the cryostat,
taking a chance on aerosolizing some nasty bug?
Is the 30 seconds you save in using freezing spray worth the exposure to TB or
goodness-knows-what?
We use a simple metal heat extractor (comes standard in our Leicas) and
Hi Anne - Interesting question. Our Human Resources department keeps records
of annual reviews, which include annual competencies, for 10 years following
the separation of the employee from the system.
I'm curious what others are doing, and does it vary from state to state. Terri
Terri L.
Just a note: The liability of having a interviewee cut themselves operating an
instrument that they have not been signed off as competent is HUGE. I think
your company/labs legal team would say absolutely not.
It would be nice to assess their cutting and other skills ahead of time, but
that is
Heads up, Histo peeps!
We have 2 ultraView kits, both from the same lot #F03020 that have extra plugs?
Black things? Floating around in the Multimer reagent.
Please check your kits carefully.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon
Hi Deanne -
Hope this will help
For our Ventana Ultra, we use Leica / Surgipath Apex Superior Adhesive slides.
We use de ionized water in our baths at 45C ish, no additives.
We never "double-dip" our slides (meaning that if we mount a control on the top
of the slide, we turn the slide upside
Just a few words on gross ventilation. To be really efficient, it should be
downdraft ventilation with appropriate sink access. Hoods with lowered sashes
obscure working vision needed, are horrible to clean and disinfect. There
should be no excuse for not budgeting an appropriate downdraft
We are down to < 0.1%, but I think you have to take into account your grossing
staff. We are just medium sized and have an outstanding PA. Our 2 new Leica
6025 processors have also made a big difference. I suspect if you are a
teaching hospital with residents grossing, there would be a
The best thing to do would be to contact either your city chemist or the state
EPA inspector to be sure to be in compliance with all local regulations. A
reducing agent, such as sodium metabisulfite, sodium sulfite or sodium
thiosulfate can be used to turn CrO3 into the less toxic Cr(III)
Hi Alison -
I've used both stainers and like both of them a lot. Both were super reliable
and easy to use. However, coverslipping is a different story.
I've used both film and glass. About film - super quick, super easy, but - the
purity of the xylene used to coverslip from film must be
Hi Barbi -
We are somewhere in-between with our ER/PR Controls. When performing lot to
lot validations, we use our in-house control, which only has a 0 and a 3+, BUT
we also use a commercially prepared control that contains 4 cores, 0. 1+, 2+,
3+. Once the new lot of antibody has been
To those of you who wrote in support of my comments, thank you sincerely.
To the Company's credit, I did receive a call of apology and a listening ear
concerning the ongoing problems associated with the dispensers. I sincerely
hope they understand the frustration that such a problem can cause.
Such a wonderful insight into the thought process of Roche. Accused of bashing
when I am telling the truth about failed lots when they claim the problem is
fixed.
If only they would put that much effort into repairing the problem and
fostering better customer relations, than how to market
Another one!!!
Our Her2 antibody dispenser failed, LOT #E22628
This one "supposedly" FDA approved.
Roche, why do you continue to lie to consumers of your product? You claim
you've "fixed" the problem but your Ventana dispensers DON'T WORK!
This is patient care! Why don't you care about the
Cleaning supplies may be stored under the sink, but since "things" have a way
of migrating there as well, we just have a blanket rule, nothing under the
sink. Of course, this would never occur in the Histology Department, LOL. The
space is actually locked off.
Terri L. Braud, HT(ASCP)
Anatomic
We have another dispenser failure. Ki-67, Cat# 790-4286, Lot# E17347, Failed
on the 9th to last dispense.
The plunger was very "sticky" and difficult to depress.
Roche, are you listening???
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon
We still have sticky issues, especially with the 250 HP dispenser, but there
have been others. I have given up on Ventana. They don't fix it, don't care,
and make you jump through hoops to get help. I'm STILL waiting on my refund 3
months ago from the HP recall where we had to repeat 78 HP
Hi Histo Peeps - I'm trying to find the brand of paraffin block storage
cabinets that have a little more height clearance than the traditional
Tissue-Tek block storage cabinets. They were shown to me about 3-4 years ago,
but I've misplaced the literature. I think they were blue? Vendors
My sympathies. I wish we had never bought the Ventana Ultra. The constant
failures of the dispensers has caused such a headache, and the documentation
needed before they will even agree to replace one reagent, and then the fight
to obtain a credit for the entire test, not to mention wasted
LIS recommendations - I LOVE CoPath! Either version, but I am currently using
the SunQuest version 6.1.
Loads of flexibility. You can use it at it's most simplest, or add in all the
bells and whistles. Easy to build and maintain, with well-established
instrument interfaces and voice
Just call BioCare Technical service. They have a complete procedure for hand
staining. I know because I used it in their facility when I went for training
on their Flx.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook,
I find it very peculiar to be lectured by a Roche representative on including
reagent management when calculating stain costs, considering Roche's yearlong
ongoing issues with reagent supply problems and massive recalls. I've yet to
have one month with no reagent supply problems from
Hi Paula -
I use duplicate microarrays (TMAs), each with at least 10 positive cases and 10
negative cases, one TMA slide on each instrument for each antibody.
Passed CAP with no problems. Hope this helps. Terri
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer
Our repeat limits differ. If it is due to tissue falling off, we will try at
least 3 times
If it is due to unexpected, but within control, we will repeat it 2 times
Then if it is still problematic for the above 2, we will send out the block for
staining.
If it is due to technical error (wrong
We wash by hand, but do use some disposables (pipets, etc). We make one final
DI wash, then check the pH for residual detergent. Record pH per batch washed.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph:
I agree with Jennifer - Just another recommendation for BioCare and their
reagents. They are a super company to work with and have excellent quality and
quality control on their reagents.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon
Remember that contamination can also be by skin absorption. With that in mind,
we provide thick Nitrile gloves with instruction to change them at least every
30 minutes, or less if they show any signs of deterioration. We have great
ventilation and beyond that, when we wear our fume badges,
Here is the approximate chemical make-up of LTP with Phenol. (LTP stands for
Long Term Preservation)
Ethanol 48.%
Formaldehyde 20.%
Ethylene Glycol 4.%
Phenol 11.%
Glutaraldehyde 4.%
Due to the presence of Ethylene Glycol, I would let the tissue set in a
separate container of 10% Neutral
I have never (3 different places, 30+ years) heard of requiring special stains
to be performed under a hood. Certainly, there are a few reagents that might
need benefit from a hood (concentrated Ammonium Hydroxide) but generally, they
are performed on an open counter next to a sink. If you
I'm with Tim Morken on this one. The variability of antigenicity in storage is
so wide open, and there really is no recent data, so we just make a point of
educating our techs on not wasting tissue/levels during sectioning. If the
techs feel that the residual tissue in the block is in danger
In the past, I've used a rapid acetylcholinesterase on frozen material, but we
never used Osmium to enhance staining. I've also used IHC on FFPE sections
using s-100 and peripherin. Works great, but it's been awhile, so I don't know
if there is something better/easier out there now.
Terri L.
We used US Biomax TMA CO804A to validate our MMR antibodies. We sent one set
to the reference lab where ours were currently being stained, and then stained
the other set with the 4 Antibodies. Compared the staining of the 2 and done!
I had also called CAP in insure that this method would be
There is an easy method to track batch controls using a blank slide. For each
case stained, file a blank slide that has been labeled with the actual case
number, the stain, and also the date of the control slide. Easy peasy. I
published this method in Histologic in Aug. 1995 and it has stood
On Date: Mon, 23 Jul 2018 18:31:48 -0400
From: Mary Ann
Subject: [Histonet] Positive PAP
Mary Ann wrote "Help! My pathologist has asked that a positive patient be run
down with our PAP stain for QC.
Point me to a reference to counter this request."
Hi Mary Ann - First of all, my sympathies.
We are using a Leica 2235 and love it. Manual or Auto mode, the control panel
is on a moveable pad, so can be used anywhere. It has a foot pedal if you like
that too! The techs took to it like a duck to water and it cuts like butter!
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Are you referring to a Pap stain control? If so, we run a self made buccal
smear every day to check for stain quality.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care,
Just an idea. Why not use SEM? It seems like it would be so much easier.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal
2. MMA or Epoxy Embedding
For all stains, we report the control verification on the patient report when
reporting the stain results, and we run negative tissue controls for our
organism stains, AFB and GMS
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
We use:
1. Report TAT
2. Discrepant pathology reports
a. Internal vs External report correlation
b. Frozen section vs Final diagnosis correlation
c. Amended reports
I liked the idea of piece count discrepancy or even poorly processed blocks. I
When will this company ever learn? I'm at my wit's end with failed dispensers
from Roche Ventana. The volume of time I've wasted on this issue with this
company is monumental. They refuse to give true tracking information on the
dispensers. We have a relatively new Ultra. The instrument is
Hi Nancy - Please see my answers filled in below. I hope that helps.
Sincerely, Terri
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal
Message: 1
Date:
We use a backup generator for power outages, and also, all critical instruments
are plugged into an appropriate sized UPS (Uninterrupted Power Supply) to hold
them for about 2 hours of uninterrupted power until the generators kick in.
Basically what Allan Wang said in his reply.
Just remember
Have you tried Polyphenyl Yellow 8G instead of Tartrazine? Just a suggestion
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal
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