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Message: 3
Date: Mon, 5 Nov 2018 16:44:23 + (UTC)
From: Laurie Redmond
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] PAS Stain
Does anyone else out there use warm water to rinse the PAS slides after the
Schiff's reagent? Can anyone comment on whether using warm water vs. cold
water would have any affect on the stain?
Laurie Redmond HT (ASCP)
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Subject: Re: [Histonet] PAS Stain
Amylase (diastase) for the PAS stain queries:
Whatever happened to spitting on the slide (30 min at room temperature)?
John Kiernan advises "thinking of lemons and drooling into a small beaker"
though I'd advise chewing on a rubber band for a few se
As I see it, there are 3 main objections about using human saliva as an amylase
source.In order of importance they are:1- you will never know the actual
concentration of the amylase and this will produce reproducibility problems.2-
along with the saliva you will introduce bacteria that may end
n via Histonet" <histonet@lists.utsouthwestern.edu>
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, May 5, 2016 5:30:22 PM
Subject: Re: [Histonet] PAS Stain
Hello All! I've served in laboratory medicine for well over 35 years and in my
early years we did indeed spit on the slides
stonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS Stain
You clearly don't know your histo history. The reason we know that H pylori
exists is because a Scientist, Dr. Barry Marshall wanted to prove bacteria
caused ulcers and not stress. No one believed him. So he took the organisms
from a p
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS Stain
Only a crazy Aussie would do this!!
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University
@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS Stain
You clearly don't know your histo history. The reason we know that H pylori
exists is because a Scientist, Dr. Barry Marshall wanted to prove bacteria
caused ulcers and not stress. No one believed him. So he took the organisms
from a patient, mixed
, Westmead
NSW 2145, AUSTRALIA
-Original Message-
From: Ray via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, 6 May 2016 4:48 AM
To: Richmond, Bob
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS Stain
An excellent point. For anyone wanting to investig
Subject: Re: [Histonet] PAS Stain
I cannot believe any scientist would advocate such a non-uniform method
as spitting on a slide.
Buy a bottle of what ever enzyme and use a reproducible buffer and
temperature.
Geoff
On 5/5/2016 3:19 PM, Anne Murvosh via Histonet wrote:
> Yes, spitting is the tr
... :)
Claire
From: Ray via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Thursday, May 05, 2016 1:48 PM
To: Richmond, Bob
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS Stain
An excellent point. For anyone wanting to investigate-simply do
the best. A fun experiment Anne
-Original Message-
From: Bob Richmond via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, May 05, 2016 11:36 AM
To: koelli...@comcast.net
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS Stain
Spokane Ray points out
Subject: Re: [Histonet] PAS Stain
Spokane Ray points out something I've wondered about for years - can just
anybody spit on the slide and remove the glycogen? I've never heard of any
variation, but the number of people I've asked is very limited. This
reference:
http://www.ncbi.nlm.nih.gov/gene/276
@comcast.net
Cc: "Histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>
Sent: Thursday, May 5, 2016 11:35:42 AM
Subject: Re: [Histonet] PAS Stain
Spokane Ray points out something I've wondered about for years - can just
anybody spit on the slide and remove the g
<histonet@lists.utsouthwestern.edu>
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, May 4, 2016 1:02:48 PM
Subject: [Histonet] PAS Stain
Hi Histonetters, I'm hoping someone can help me troubleshoot our PAS diastase
method. We have been digesting the tissue in 0.5% diastase of malt in a 60
degre
rises above 37C you will see a decrease in enzyme activity and
then none at all.
Jennifer
From: Joanne Clark via Histonet <histonet@lists.utsouthwestern.edu>
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Date: 05/04/2016 01:04 PM
Subject:
t@lists.utsouthwestern.edu" <
> histonet@lists.utsouthwestern.edu>
> *Sent: *Thursday, May 5, 2016 11:10:40 AM
> *Subject: *Re: [Histonet] PAS Stain
>
>
> Amylase (diastase) for the PAS stain queries:
>
> Whatever happened to spitting on the slide (30 min at room
d via Histonet" <histonet@lists.utsouthwestern.edu>
To: "Histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>
Sent: Thursday, May 5, 2016 11:10:40 AM
Subject: Re: [Histonet] PAS Stain
Amylase (diastase) for the PAS stain queries:
Whatever happened to spitt
Amylase (diastase) for the PAS stain queries:
Whatever happened to spitting on the slide (30 min at room temperature)?
John Kiernan advises "thinking of lemons and drooling into a small beaker"
though I'd advise chewing on a rubber band for a few seconds.
He notes that alpha amylase is
Box 4
West Point, PA 19486
215-652-9750
215-993-0383 (fax)
philip_man...@merck.com
-Original Message-
From: Joanne Clark via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, May 04, 2016 4:03 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] PAS Stain
Hi
[mailto:histonet@lists.utsouthwestern.edu]
Gesendet: Mittwoch, 04. Mai 2016 22:03
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] PAS Stain
Hi Histonetters, I'm hoping someone can help me troubleshoot our PAS
diastase method. We have been digesting the tissue in 0.5% diastase of malt
in a 60
Hi Histonetters, I'm hoping someone can help me troubleshoot our PAS diastase
method. We have been digesting the tissue in 0.5% diastase of malt in a 60
degree oven for 30 minutes, but do not see the glycogen being digested out. I
have tried alpha amylase and beta amylase also without any
: [Histonet] PAS stain
Message-ID: 16f1d792-cd79-458e-9d25-493d8bc0c...@aol.com
Content-Type: text/plain; charset=us-ascii
Just a question...what kind of hematoxylin do you use as a counter stain for
your PAS stain and do you use a differentiator?
Sent from my iPad
From: Mica [faith14...@aol.com]
Sent: Tuesday, 2 June 2015 1:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] PAS stain
Just a question...what kind of hematoxylin do you use as a counter stain for
your PAS stain and do you use a differentiator?
Sent
Just a question...what kind of hematoxylin do you use as a counter stain for
your PAS stain and do you use a differentiator?
Sent from my iPad
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Hi
We have been doing a PAS stain on the same control block and same
reagent supplier for a long time.
Yesterday when we ran the slides, they failed to stain
We opened new bottles of Periodic Acid and Schiff's Reagent and recut
fresh controls
Still, we are unable to get any staining.
Were the new bottles from the same lot?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Diana
McCaig
Sent: Wednesday, October 06, 2010 11:03 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] PAS
-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Diana
McCaig
Gesendet: Mittwoch, 06. Oktober 2010 17:03
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] PAS STAIN
Hi
We have been doing a PAS stain
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Wednesday, October 06, 2010 9:06 AM
To: Diana McCaig; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] PAS STAIN
Were the new bottles from the same lot?
-Original Message-
From: histonet
I make my Schiff's from scratch and store it in the refrigerator.
I use a minimum amount of charcoal and I heat it in the oven at over 100
C the night before to be sure it is nice and dry.
I make periodic acid fresh that day.
I make bisulfite rinses fresh that day.
Aqueous formalin for 48 hours
.
8201 East Riverside Dr. Bldg 4 Suite 100
A= ustin, Texas 78744
(512)386-5107
Original Message
Subject: Re: [Histonet] PAS STAIN
From: Geoff McAuliffe [1]mcaul...@um= dnj.edu
Date: Wed, October 06, 2010 1:28 pm
To: Diana McCaig [2]dmcc...@ckha.on.= ca, Histonet
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