Re: [Histonet] processing artifact - delayed start

n, Charles) -- Message: 1 Date: Mon, 5 Feb 2024 13:07:41 + From: "Bacon, Charles" To: Verizon wireless , "histonet@lists.utsouthwestern.edu" Subject: Re: [Histonet] Processing artif

Re: [Histonet] Processing artifact - delayed start

We have had 2 reasons why we saw processing issues like this: 1. I recently found out on our VIP 5 they did not turn the level sensors on during install. These sensors are known to error so often, Sakura tells technicians to set the default to off. All the processor can sense is pressure and

Re: [Histonet] Processing artifact - delayed start

I would check the level of the formalin after it has been pumped into the retort. I will wait till the pics are posted Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children’s Hospital at Westmead (Retired) Adjunct Fellow, School of

Re: [Histonet] Processing artefact??

Since images cannot be attached, if any of you feel you have some experience that I may find helpful...please email me directly and I will attach the image to my reply. Thanks again, *Greg Dobbin* Chief Technologist Anatomic Pathology Queen Elizabeth Hospital Charlottetown, PE, Canada

Re: [Histonet] Processing Protocols

Hi Jessica My advice would be to process your small biopsies during the day allowing depending on the times you use at least 2 runs per day. One in the am and one in the pm. Then run your large blocks over night as per usual. Any late arriving small biopsies can be run on the am run the

Re: [Histonet] Processing Protocols

We have encountered a similar problem with one of our two processors is down. Shortening the longer schedule produced underprocessed fatty tissues and over processed biopsies, so we run the biopsies on the short schedule overnight, run a quick clean cycle, then the larger tissues on the 10hr

Re: [Histonet] Processing correction

Recovering Tissue That Has Dried Out After Malfunction of Tissue Processor  | | | | || | | | | | Recovering Tissue That Has Dried Out After Malfunction of Tissue Processor | | | | Sent from Yahoo Mail for iPhone On Monday, March 1, 2021, 2:10 PM,

Re: [Histonet] Processing Schedule- ASP-6025

What are you using for your lab's own control. Was that tissue processed recently? Do you have normal tonsil that you process in-house regularly that also stains poorly? On Wed, May 8, 2019 at 9:16 AM Greg Dobbin via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Great point John! >

Re: [Histonet] Processing Schedule- ASP-6025

Great point John! We do attempt to have all of the specimen handling for our control tissue, match that of our specimens. However, tonsillectomies at our hospital are only performed on Thursdays. So we fix them for 24hrs (until Friday) then process overnight and remove from the processor on

Re: [Histonet] Processing Schedule- ASP-6025

The processing schedule looks fine. I am thinking that if you are having no problems with morphology on the H processing is not the issue. The extended period in the warming draw is a good hypothesis. I do have a question. How long was that particular piece of tonsil kept in formalin before

Re: [Histonet] Processing Schedule- ASP-6025

Processing seems adequate. After processing, how long do they sit in the embedding centre block holding tank before embedding? We found that quite a few antigens were affected when we stored control tonsil in the embedding centre (dry) at 60oC for a few days before embedding. In summary:

Re: [Histonet] Processing Veterinary Samples

The vet samples can be processed just any other animal tissue whether they be human or rat. People is animals too. Formalin fixed there are no special safety issues. E. Wayne Johnson DVM Enable AgTech Beijing ewj Email:e...@pigs.ag Signature is customized by Netease Mail Master On 04/16/2019

Re: [Histonet] processing Dosophilla flies

Hi Processing them , how? To Pwax? To snapfreeze? To resin? Kind regards Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge  Kings College London London SE1 1UL   020 7848 6813 ___ Histonet

Re: [Histonet] Processing cystic tissue

Charles Riley HT, HTL(ASCP)CM, a Mohs histopathology coordinator, asks: >>Can anyone give me an idea how they process cystic tissues? The normal tissue processes extremely well on my current protocol but the cystic areas just are too soft to cut. Any tricks anyone can provide to process better or

Re: [Histonet] Processing cystic tissue

Charles: I usually get out as much of the cystic material as I can without damaging the cyst wall itself. That is all the docs are looking at anyway. I am assuming you are referring to skin cysts, not ovaries, etc. Claire From: Charles Riley via

Re: [Histonet] processing cell blocks in formalin

Yes, I will send you the procedure that we use. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour

Re: [Histonet] Processing FFPE tissue without alcohol

Will they be able to do IHC stains? -Original Message- From: Wanda Shotsberger Gray via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Saturday, March 26, 2016 12:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processing FFPE tissue without alcohol While the

Re: [Histonet] Processing issues

Hey Charles, Why are the first 2 steps of formalin that long? If you make sure your tissue is well fixated, then there is no need for such long fixation in the machine. You could use step 2 for just washing the formalin out, so for only like 5 minutes. You could use that time, to add to other

Re: [Histonet] Processing issues

Not knowing what issues you have, I suggest you look to your samples for processing. Reduce thickness and the shorter times will work. 2-2.5 mm thick. Standardize fixation before placing in tissue processor. What time is the processor started and what time does the tech remove? You may be able

Re: [Histonet] Processing cytology and cell block protocols

We are a similar sized hospital with an 8,000/yr Surgical load of mixed large and small cases. We process 1000 Non-gyn Cytology cases, assist with FNA collection in Interventional Radiology. We also assist with about 130 bone marrow collections, including smears and processing. There is

Re: [Histonet] Processing of breast tissue

Do you using "freezing spray" on these specimens? Direct spray can cause the tissue to appear burnt. From: Charles Riley via Histonet To: histonet@lists.utsouthwestern.edu Date: 01/05/2016 06:00 AM Subject:[Histonet] Processing of breast

Re: [Histonet] processing cycle

Hi Allison, we doubled the times of the regular processing protocol beginning with longer absolute ethanol, intermedium and paraffin. Our regular protocol takes 13 hours and the fatty-protocol takes 17 hours. We start it at about 2 pm with endtime at 8 am. So breast tissue is mostly embedded at

Re: [Histonet] Processing Validation

Would love to see also. Michael Ann On 6/15/15, 2:18 PM, Leslie, Mary mary.les...@tricore.org wrote: Does anyone have a processing validation procedure they would like to share? Thank you! Mary Leslie ___ Histonet mailing list

Re: [Histonet] processing problem

Having morphology problems when re-processing a tissue after an accident as you had is the unavoidable consequence. The problem is rooted in the fact that usually you remove the paraffin using the tissue processor cleaning cycle and that is a very harsh method (albeit the most expeditious). If

Re: [Histonet] processing problem

I am afraid your tissue might be 'toast'. I tried to reprocess from that same issue and things did not go well. We were processing mouse tissue, which is dry enough, but then when we put the tissues back through xylene and into 100% and back again it got even worse. We had to just get what we

Re: [Histonet] processing problem

Hi Martha I think that Caroline is correct but you have nothing to lose by removing the wax and then trying to dehydrate and so on. A more gentle way after removing the wax and dehydrating is to use chloroform instead of xylene and hand processing. Chloroform is much more gentle although tissue

RE: [Histonet] processing tissue with silicone mesh in it

Patsy- We routinely process and section many meshes in paraffin. I would suggest processing a sample, embedding and sectioning to see if you can get good sections. It is worth a try. Joe Saby NAMSA Sent on the new Sprint Network from my Samsung Galaxy S®4. Original message

Re: [Histonet] Processing:

This depends on so many different factors, however, I prefer a frequent rotation over a complete change. Do what is best for your tissue! Sent from my iPhone On Jan 13, 2014, at 9:46 AM, Jb craiga...@gmail.com wrote: I have one tech telling me that when the entire processor is changed the

RE: [Histonet] Processing:

This gets me back to another recent topic, soaking the blocks. I've seen this a little in the past, just soak them on an ice block,tray for a couple minutes and you'll be fine. To me, another indicator would be that if you're getting dry tissue when changed but not later could there be some

Re: [Histonet] Processing Problem

A sample 2mmx2mmx1mm is quite small and there should not be any problems with either fixation of processing but there is where the problem my reside. 1- fixing in 10% formalin: is it neutral buffered? For such a small piece 24 hours will be enough but to be absolutely sure try leaving the pieces

RE: [Histonet] processing eyes

Patsy, 18-24 hours in Davidson's followed by 70% ethanol prior to processing. Some people also store the eyes in 10%NBF. I will attach a schedule for you off line. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010

Re: [Histonet] processing eyes

We transfer eyes out of Mod Davidsons after 24 hours and process with the other soft tissues in formalin- why transfer to alcohol? We have beautiful eye sections. Jackie O' Sent from my iPhone On Aug 8, 2013, at 3:12 PM, Bea DeBrosse-Serra bdebrosse-se...@isisph.com wrote: Patsy, 18-24

Re: [Histonet] Processing Fumes

Stop using xylene! René J. From: MaryK Mendell kmend...@goldbergmd.net To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Friday, August 2, 2013 7:58 AM Subject: [Histonet] Processing Fumes I run a very small Derm lab and starting

Re: [Histonet] Processing Fumes

I have the same processor. If you remove the back panel there is a drip jar for waste just inside. It might be getting full. Tony Auge HTL (ASCP) QIHC Cell: (651) 373-4768 Email: tony.a...@gmail.com On Fri, Aug 2, 2013 at 4:58 AM, MaryK Mendell kmend...@goldbergmd.netwrote: I run a very small

Re: [Histonet] Processing Guinea Pig

Heather-   I do not know why, but to properly process guinea pig tissues you need a much more rigorous program than what would work for mice. It is very easy to over process mouse tissue.  Even rat tissue needs more processing.  Guinea pig tissue need a program designed for processing larger

Re: [Histonet] Processing Guinea Pig

Heather, Looking at your protocol for processing the kidney and heart tissue I can't figure out why it is mushy especially if you are putting it in the cassettes so thin and it has had a chance to fix well before processing. In fact, looking at your protocol I might think that your tissue might

RE: [Histonet] Processing thin slices of mouse cerebellum

http://www.cellpath.us.com/magento/cellpath-on-line-shop/specimen-processing-and-embedding/small-biospy-processing/cellsafe/cellsafe-biopsy-capsule-blue.html I find them very good for brain slices. There is a deeper and a shallower space, depending on which way they are folded. Carl Hobbs FIBMS

RE: [Histonet] Processing thin slices of mouse cerebellum

Hi Kathleen, Several years and a couple employers ago we attached small biopsies (for proper orientation) to small squares of cucumber (which had been dehydrated in several changes of alcohol) with a egg-albumin/glycerol mixture. The bx/cucumber unit was embedded together after processing and

Re: [Histonet] processing protocol using isopropyl alcohol, mineral oil paraffin

I'd like to be included in the protocol processing exchange too! Best Maria Mejia On Dec 5, 2012, at 10:55 AM, Lynette Pavelich wrote: Yes, please share! Thanks! Lynette From: histonet-boun...@lists.utsouthwestern.edu

Re: [Histonet] Processing issue

That will depend on the tissue and the size/thickness of the specimens. Appreciate how they turned out and you will have the answer to your question. At least for me it is very difficult to answer your question without knowing the tissue characteristics. René J.

Re: [Histonet] Processing Autopsies

Brains in particular need to be fixed real well If it's a whole brain what I've done is hang the brain by a mesh or strings into a large brain bucket so it's not touching the sides or bottom. Fix for few days then get you sections. I'd go textbook on the 3 mm thick sections for processing and

RE: [Histonet] Processing adipose tissue

the last alcohol and the first paraffin wax bath. René J. --- On Mon, 1/30/12, Jerry Ricks rosenfeld...@hotmail.com wrote: From: Jerry Ricks rosenfeld...@hotmail.com Subject: RE: [Histonet] Processing adipose tissue To: histonet@lists.utsouthwestern.edu Date: Monday, January 30, 2012, 7:15 PM

RE: [Histonet] Processing adipose tissue

...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, January 31, 2012 8:34 AM To: histonet@lists.utsouthwestern.edu; Jerry Ricks Subject: RE: [Histonet] Processing adipose tissue I agree that 21 hours is too much, but using isopropyl alcohol does not a clearing step because isopropyl alcohol

RE: [Histonet] Processing adipose tissue

: David Burk david.b...@pbrc.edu Subject: RE: [Histonet] Processing adipose tissue To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 31, 2012, 9:51 AM René, Thanks very much for your input on this matter.  I also want to go ahead and thank the other two (Jerry and Karen) who have chimed

RE: [Histonet] Processing adipose tissue

! Tracy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jerry Ricks Sent: Monday, January 30, 2012 5:15 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing adipose tissue Hi David, 21

RE: [Histonet] Processing adipose tissue

Hi David, 21 hours in isopropyl seems likw ea lot, and I don't see any clearing step to remove the IPA. I've been using Slide Brite instead of Xylene, but I see that Rene Buesa published a study indicating that mineral oil is the cat's meow for xylene substitutes.

Re: [Histonet] Processing of derm specimens

How many 100% do you have. I have 3. This is where the dehydration comes in. The 100 takes all the mositure out of the specimen. So this step is critical. Water and xylene are not soluable so if the specimens are not getting dehydrated properly, the xylene will not penertate the specimens

RE: [Histonet] Processing of derm specimens

That processing schedule should be fine for skin samples, we add an additional 100% alcohol step so we have three absolute steps at 1 hour each (I would remove one 95%). Thickness of your samples is also important they should be around 3mm in thickness if they are thicker than that they may

Re: [Histonet] Processing

Set your formalin at 45ºC, dehydration at room temp with vacuum/pressure and the paraffin as usual. René J. --- On Wed, 7/6/11, Nicole Schuster njbate...@yahoo.com wrote: From: Nicole Schuster njbate...@yahoo.com Subject: [Histonet] Processing To: histonet@lists.utsouthwestern.edu

RE: [Histonet] Processing

We heat from 38-40 degrees for all reagents except the paraffin, which is heated to 60 degrees. I don't use any heat at all on my small biopsies. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On

RE: [Histonet] Processing schedule for fatty tissues

Please respond to everyone. I would like to have the information also. Thank you, Carol -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Friday, June 10, 2011 4:23 PM To:

Re: [Histonet] Processing schedule for fatty tissues

The best approach is to increase the fixation time to make sure that the tissues are really fixed. Then cut the dehydration times of the lower alcohols by 20% and add that 20% of saved time as increase in the clearing agent (supposedly xylene or alkane substitute) times. René J. From: Sheila

Re: [Histonet] Processing Mouse Pancreas

Develop a good general protocol for animal tissue. You cannot have dedicated protocols for every type of tissue. René J. --- On Fri, 6/3/11, Stoll, Kathryn kst...@mcw.edu wrote: From: Stoll, Kathryn kst...@mcw.edu Subject: [Histonet] Processing Mouse Pancreas To:

RE: [Histonet] processing times for skin specimens

Carol We are a research lab but we have always processed our skin samples on longer processing cycles, even the punch biopsy samples. The shaves might be different, if I wanted to cut down I would start with how you normally process them and then cut down at 5 minute increments to start and see

Re: [Histonet] Processing animal fat

My question is directed specifically to veterinary histologists or histologists who also do a fair amount of animal processing. We are having a terrible time processing pig fat. We had problems previously, but thought we had solved them. This latest project (pig skin with a lot of fat attached)

RE: [Histonet] Processing animal fat

@lists.utsouthwestern.edu Subject: Re: [Histonet] Processing animal fat My question is directed specifically to veterinary histologists or histologists who also do a fair amount of animal processing. We are having a terrible time processing pig fat. We had problems previously, but thought we had solved them

RE: Re: [Histonet] Processing animal fat

The specimens can be large, but we trim so the thickness is less than 5mm. -Original Message- From: Marcum, Pamela A [mailto:pamar...@uams.edu] Sent: Thursday, March 17, 2011 4:02 PM To: Sherwood, Margaret ; histonet@lists.utsouthwestern.edu Subject: RE: Re: [Histonet] Processing animal

Re: [Histonet] Processing animal fat

...@partners.org Subject: Re: [Histonet] Processing animal fat To: histonet@lists.utsouthwestern.edu Date: Thursday, March 17, 2011, 3:57 PM My question is directed specifically to veterinary histologists or histologists who also do a fair amount of animal processing.  We are having a terrible time

Re: [Histonet] Processing animal fat

Peggy, Is your whole program one hour or is each station one hour? We had a project here a few years ago that we called the bacon project because we had whole chunks of pig skin with an implant and lots of fat in between the layers. It was fixed for a couple days in 10% NBF and then processed

Re: [Histonet] Processing animal fat

We trim to 2mm thick. Jan Shivers UMN Vet Diag Lab - Original Message - From: Liz Chlipala l...@premierlab.com To: Sherwood, Margaret msherw...@partners.org; histonet@lists.utsouthwestern.edu Sent: Thursday, March 17, 2011 3:02 PM Subject: RE: [Histonet] Processing animal fat 5mm

RE: [Histonet] Processing animal fat

, Margaret Sent: Thursday, March 17, 2011 2:29 PM To: Grantham, Andrea L - (algranth); HISTONET Subject: RE: [Histonet] Processing animal fat Every station is 1 hour, and our processing program is similar to yours: we run the progam overnight, so specimens sit in formalin for a delayed start. Then 50

RE: [Histonet] processing histogel

You could try asking the authors of the paper for their protocol. Nalini -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Luciana Bastos Sent: Monday, February 14, 2011 5:06 AM To:

RE: [Histonet] processing histogel

Luciana You process histogel samples essentially the same way you process regular tissue samples. I'm assuming you will be dealing with a about a 1 cm cube sample that you want embedded into the histogel and then you would then slice the histogel cube (with the collagen construct) into 3 mm

Re: [Histonet] Processing rodent tissue

John: Some labs uses processing metod with isopropanol and mineral oil with very good results. Here is our manual protocol for rodent tissues: 1. Isopropanol 70% 0.5 h 2. Isopropanol 80% 0.5 h 3. Isopropanol 95% 0.5 h 4. Isopropanol 99% 0.5 h 5. IM 5:1 50oC 1 h 6. IM 2:1 50oC 1 h 7. Mineral oil

Re: [Histonet] Processing lavages into cell pellets...

Hi Jennifer I realise there are products like Histogel about of which I have no knowledge however I have been using agar for over 30 years because I can get it easily and gratis from our microbiology department. My experience is to spin down the cells, remove the supernatent, then add

Re: [Histonet] Processing of fatty breast tissue

You have assumed that processing is correct, but shrinkage is always caused by too fast dehydration, therefore the processing is not adequate. René J. --- On Wed, 3/3/10, Dinesh Sariya sari...@hotmail.com wrote: From: Dinesh Sariya sari...@hotmail.com Subject: [Histonet] Processing of fatty

Re: [Histonet] Processing small gi biopsies, skin lesions, currettings

Thanks for throwing this one out there. I am also interested in this topic. Can you please post replies to the net? Thanks !!! :-) --- On Thu, 11/5/09, Vickroy, Jim vickroy@mhsil.com wrote: From: Vickroy, Jim vickroy@mhsil.com Subject: [Histonet] Processing small gi biopsies, skin

Re: [Histonet] Processing Whole prostate

The important aspect is fixation. Let your PA slice the whole prostate and place them in the so called mega-cassettes in a plastic container over a vortex so they rotate inside the NBF for at least 24 hours. After that just use the longest of your protocols. If you have one that has worked well

Re: [Histonet] Processing Fat for Paraffin

Paula: For me the times (2 h/each), your dependable instrument and the schedule seem to be OK, but I am not so sure about the Citrisolve because this product (1.4 more expensive than xylene) is made of 91% of water and surfactants + 9% d-limonene and I am not very confident that such a clearing

Re: [Histonet] processing artifact

Gloria: Frequently the problems that appear in the sections are attributed to processing defects when they usually originate before processing, during fixation. Small pieces of tissue left to dry before being placed in the fixative. You write that sometimes happens to one biopsy in a group of

Re: [Histonet] processing v-e-r-y tiny samples

Shandon - or whatever they're called this week- sell nylon mesh biopsy bags. These are flat bags, sealed on 3 sides. I have found that if you cut one corner off (ie, a oblong piece with 2 sides still sealed) just a tiny bit bigger than the cassette, you can open the bag, like a book, insert the

RE: [Histonet] processing v-e-r-y tiny samples

19, 2009 3:39 PM To: histonet@lists.utsouthwestern.edu; Andrea Grantham Subject: Re: [Histonet] processing v-e-r-y tiny samples I may never complain about tiny GI or bronch biopsies ever again! Andrea Grantham algra...@u.arizona.edu 3/19/2009 11:51 AM Good Morning! In keeping with the weirdness

RE: [Histonet] processing v-e-r-y tiny samples

Careful - you may be close to the mark. Thermo laid off about 75 people here in Madison last week. Claire Shandon - or whatever they're called this week- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] processing v-e-r-y tiny samples

Well, you could always hand process them in test tubes. Jerry Ricks Research Scientist University of Washington Department of Pathology Date: Thu, 19 Mar 2009 16:39:14 -0400 From: bryan.wat...@parkview.com To: histonet@lists.utsouthwestern.edu; algra...@u.arizona.edu Subject: Re: [Histonet

RE: [Histonet] processing v-e-r-y tiny samples

-boun...@lists.utsouthwestern.edu] On Behalf Of Bryan Watson Sent: Thursday, March 19, 2009 4:39 PM To: histonet@lists.utsouthwestern.edu; Andrea Grantham Subject: Re: [Histonet] processing v-e-r-y tiny samples I may never complain about tiny GI or bronch biopsies ever again! Andrea Grantham algra

RE: [Histonet] Processing 3-dimensional cell cultures into paraffin

] On Behalf Of Monfils, Paul Sent: Tuesday, January 13, 2009 12:46 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing 3-dimensional cell cultures into paraffin I have processed and embedded cells grown in/on agar, or post-embedded in agar or agarose, many times. It really

Re: [Histonet] Processing mouse seminal vesicles

Mouse tissues, any one, will dry out too much with ethanol and xylene. I advise you to stop using ethanol and xylene and substitute BOTH with iso-propanol at the concentrations and times you are using now. Instead of the first paraffin, use a mixture 1:1 of iso-propanol and paraffin, followed by

Re: [Histonet] Processing mouse seminal vesicles-thanks!

Well, I have a number of suggestions now; once I get past the holidays I'll start trying them out when the next batch of samples comes in. We provide histology and pathology services for our dept and university, so it's all animal tissue. Thanks again to everybody, and I'll let you know how