t 60-100+ blocks a day and probably
20-40 H&E slides a day. Having to keep them for 2-3 weeks' time can give us a
considerable inventory to manage.
Thanks!
Teri Johnson
Sr. Histotechnician, Decipher Urologic Cancers
___
Histonet mail
ast one we received had epredia.com in
the footer and I contacted them in the hopes of getting one. I have heard
nothing back.
Can someone please point me to a resource that can provide consistent, timely
CofAs for this product?
Thanks in advance,
Teri Johnson, Sr. Histotechnician
Decipher Urolog
Wait...what? When is Ventana p16 being discontinued?
Teri Johnson, HT(ASCP)QIHC
Manager, Clinical Trial Testing
T +1 760 516 5954
tejohn...@genoptix.com
Navigate BioPharma, Inc.
A Novartis Company
1890 Rutherford Rd.
Carlsbad, CA 92008
USA
Date: Wed, 5 Apr 2017 01:54:06 +
From: Samantha
Hi Mariam,
Mayer's is a progressive hematoxylin. Therefore you should increase your time
in the stain to get a deeper color. You might try 10 or 20 minutes and see what
works best for you.
You can do your bluing in tap water or even buffer solution rather than
ammonium hydroxide if you prefer.
Dr. Richmond,
Thank you for all your contributions to the histonet over the years. I would
have loved working with you. I know I loved learning from you.
Best wishes and happy retirement!
Teri Johnson, HT(ASCP)QIHC
Manager, Clinical Trial Testing
T +1 760 516 5954
tejohn...@genoptix.com
Hi Bernice,
Likely there is something in your TUNEL procedure that is causing a problem
with histochemical nuclear staining. If it isn't the pre-treatment, it might be
the DAPI. After all, they do bind the same structures, and the DAPI might be
winning that competition.
Teri Johnson
Ma
t is delivering the proper stain protocol every time I do a
staining run.
Best wishes,
Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA 92008
USA
Phone +1 760 516 5954
tejohn...@genoptix.com
w
Hi Histoland,
I would love to hear from folks who are currently using the Primera slide
printers in your lab. We are working towards bringing them on line (networked)
and still have not worked out the physical bugs for printing and slide jamming.
We want to know some of the issues you have work
no issues on our Ventanas.
Good luck!
Teri Johnson, HT(ASCP)QIHC
Manager Clinical Trial Testing
Genoptix, Inc.
SAN5, Rm. 2005
760.516.5954 (office)
760.516.6201 (fax)
CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for
the sole use of
djustment by the pathologists
in recognizing their particular artifacts and how they can use it to aid the
interpretation.
Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA 92008
USA
Phone +1 760 516 5954
tejohn.
uot; model comes out soon after I have purchased
one. So the life span is probably 20 years after you wished you had a different
one. :-)
Best wishes,
Teri
Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA 92008
USA
Phone
Hi Histonetters,
Can someone tell me if they do anything specific to validate their IHC markers
on decalcified specimens? If no, do you test them anyway?
Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA 92008
USA
Phone +1
that they recommend 30 seconds-1 minute
for either solution for the Hematoxylin 7211 and 7212 stain, and 20 seconds-1
minute if using the Hematoxylin 1 and 2. At 2.5 minutes, you might be overdoing
your "clarifying"!
Best wishes and good luck!
Teri Johnson
Manager, Clinical Trial T
that fixed it for us.
Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA 92008
USA
Phone +1 760 516 5954
tejohn...@genoptix.com<mailto:tejohn...@genoptix.com>
www.genoptix.com<http://www.gen
of the tissues.
I would recommend cutting the block again, air drying the slides for a time
before using heat to melt the wax prior to H&E stain and see if the artifact
persists.
http://www.cap.org/apps/docs/proficiency_testing/nuclear_bubbling.pdf
Best wishes,
Teri Joh
need to do this practice. An
inspector could argue that f) other relevant information could include received
date.
Best wishes,
Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA 92008
USA
Phone +1 760 516 5954
tejohn
ecise answer if I had an image of what
your issue looks like.
Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA 92008
USA
Phone +1 760 516 5954
tejohn...@genoptix.com<mailto:tejohn...@genoptix.com>
www.genopt
g the patient based on these results?
Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA 92008
USA
Phone +1 760 516 5954
tejohn...@genoptix.com<mailto:tejohn...@genoptix.com>
www.genoptix.com<http://ww
is more than a laboratory - we deliver actionable results to improve
quality of life.
Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA 92008
USA
Phone +1 760 516 5954
tejohn...@genoptix.com<mailto:tejohn...@genoptix.com
;t required beyond a Bachelor's degree.
I do hope you remain in the field. Your current knowledge and enthusiasm for
the discipline is a wonderful thing.
Best wishes,
Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA 92008
U
using the least
amount of coating rather than more. More doesn't always mean better adhesion,
and as Nancy Thomas reported usually ends up causing more imaging interference
and stain uptake making for a messier slide.
Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis co
lso a histology professional can tell you the extra knowledge comes in
handy in real life.
So, why not also find schools that have these programs and throw job openings
up on their bulletin board? We may not get many nibbles, but some is better
than none.
Teri Johnson
Manager, Clinical Trial Testin
sample.
Teri Johnson, HT(ASCP)QIHC
Manager Clinical Trial Testing
Genoptix, Inc.
SAN5, Rm. 2005
760.516.5954 (office)
760.516.6201 (fax)
CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for
the sole use of the intended recipient(s) and
Dear colleagues,
Is anyone available to share their grossing procedure that meets CAP/CLIA
requirements for HTs doing grossing? I appreciate your input as always.
PS. Please don't turn this into a "PA vs HT grossing" contest. BTDT
Happy Wednesday!
Teri Johnson, HT(ASCP)QIHC
M
Use a smaller font?
Teri Johnson, HT(ASCP)QIHC
Manager Clinical Trial Testing
Genoptix, Inc.
SAN5, Rm. 2005
760.516.5954 (office)
760.516.6201 (fax)
CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for
the sole use of the intended
I agree with Rachel. I would not be quite as worried about the temperature
change if indeed they maintain no higher than -13 degrees C and would not be
freeze/thawing, but what does your ice cube tray look like after about a month
or more in a frost-free freezer?
Best wishes,
Teri Johnson, HT
That sounds like a valid concern, Richard. Thank you for being an advocate for
good utilization of patient testing. What is behind their concern? Do they
think there has been a mistake with patient identification in your lab? Or are
they trying to reconcile low PSA or other discordant screening
What are the CLIA/CAP labs doing for daily H&E QC? Does a pathologist need to
review the H&E control slide daily, or do you have an ASCP certified tech
delegated to doing this?
Teri Johnson, HT(ASCP)QIHC
Manager Clinical Trial Testing
Genoptix, Inc.
SAN5, Rm. 2005
760.516.5954
Dear all,
I am changing the language to flotation bath (instead of water bath, since that
is entirely different lab equipment). I understand there is no longer a CAP
requirement to record temperatures. Is there a GLP (or GxP) requirement to do
so?
Teri Johnson, HT(ASCP)QIHC
Manager Clinical
Ex: If I need 100 ul/slide of diluted antibody and 4 slides to stain, I would
multiply both sides by 3, since multiplying it by 2 won't give you quite enough
:
1 x 3 = 3 (ul of 1:10 antibody)
194 x 3 = 582 (ul of diluent)
Not sure if I helped or confused things.
Teri Johnson, HT(ASCP)QI
I applaud labs who have moved away from Saturday rotations. But I'm really at a
loss to understand how it cuts costs? Don't most of you shift your work week
for your staff if filling a Saturday rotation? So long as you don't pay OT for
a Saturday, wouldn't the costs be the
in each change for good
infiltration.
Teri Johnson, HT(ASCP)QIHC
Manager Clinical Trial Testing
Genoptix, Inc.
SAN5, Rm. 2005
760.516.5954 (office)
760.516.6201 (fax)
CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for
the sole use
concentration is 70% or lower.
Those are the weird things that came to mind. I hope this helps,
Teri Johnson
Manager Clinical Trial Testing
Genoptix, Inc.
Carlsbad, CA 92008
CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for
the sole use of
Dear all,
For those of you using the SlideMate, can you give me a list of compatible
slides for these units?
Thanks!
Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752
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histonet-requ...@lists.utsouthwestern.edu>
Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752
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Dear Mr. Peggy,
Thank you for letting us know. It had to be a difficult letter to write, but I
am grateful for it. I will address the remainder directly to Peggy.
Dear Peggy,
Thank you for all you have done to shape the course and future of
Histotechnology. Trust that your contributions will
x27;s become a game for me to spot it.
Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752
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current customers who may have to deal
with issues from regulatory agencies. A little bit of guidance in these matters
would be quite helpful to us.
Best wishes,
Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752
Those of you having trouble with slides in the Slidemate, please respond to me
privately and let me know which ones are causing trouble for you.
Thanks!
Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752
t the goal. Instead a combination of efficiency,
consistency and excellence is expected.
If what I have written doesn't appeal to you, then please keep your other
options open.
Best wishes,
Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
85
h the manufacturer to see what they
recommend.
Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752
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to
do so. None of what we were asked to provide made any sense to me.
~tj
-Original Message-
From: Walter Benton [mailto:wben...@cua.md]
Sent: Tuesday, April 15, 2014 11:20 AM
To: Elizabeth Chlipala; Teri Johnson; 'dennis.h...@cookchildrens.org'
Cc: histonet@lists.utsouthwes
ntaminated alcohol
separate and out of the recycler. Xylene will not be removed from alcohol
during alcohol recycling so you will still have contaminated alcohol. Anything
containing xylene must be recycled as xylene (or manifested out as waste).
I hope this helps,
Teri Johnson
Manager, Histolog
Happy Friday!
I am looking for a source of anti-FLAG antibody that works in mouse FFPE
tissues.
Bonus points if it work with Ventana/Roche instrumentation.
Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752
if all your work is based on samples partially fixed in aldehyde, and partially
fixed in alcohol. Much harder to standardize that IMHO.
Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752
e (and additional references) regarding
neutralization. They are always happy to help us with these issues.
Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752
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Hi
a plastic ziplock bag prior to heating.
After heat mordant, take the bag to the fume hood and open it.
Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752
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Histonet
The solution that grows fungus is the light green counterstain.
Are you using that for your PAS and the GMS? When we did PAS for fungus, we
used light green instead of hematoxylin to counterstain.
Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
section. Honestly for us it was much
quicker than using plastics, and it gave us the ability to do regular specimen
and IHC stains on them if needed.
Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752
room temperature.
3.Prior to use, warm the agarose to 40 degrees C until completely
melted.
Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752
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wn antibodies.
Which antibody are you trying to stain and what tissue are you using?
Best wishes,
Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752
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eze platform with peltier cooling, so updating
an old cryostat for safety reasons might be another option.
Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752
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Histonet
deposition = less fluorescence.
We also use a Texas Red excitation filter on our scope. Texas Red is a
fluorophore used for labeling in IHC (among other things), and the filter is
one that will excite at that wavelength. It also excites the Alexa594
fluorophore which is what we use extensiv
t try
using gelatin/sucrose as an embedding medium. Will you be staining
free-floating sections?
Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752
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His
perfusion
using the same materials.
Ref:
http://www.leicabiosystems.com/pathologyleaders/sacrifice-perfusion-in-animal-research/
My thought is that if you see shrinkage of the material depending on perfusion
fixation methods, you will likely see it with immersion fixation.
Teri Johnson
Manager
determine is appropriate) across multiple specimens and then store them in 70%
ethanol to send. The important thing is to standardize everything you can.
Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752
activity kinetically and therefore
makes processing more efficient.
Can someone affirm or deny the efficacy of vacuum and/or pressure in tissue
processing, please?
Thank you, as always, for your wisdom.
Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
minutes prior to staining,
rinse with buffer and proceed with staining, no digestion.
Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752
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ixed control tissues processed the same and will
need to re-validate your antibody staining using those. That usually only
requires a titration run, which is what you would do with a new antibody lot
anyway.
Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Di
s an option.
3 - "Opportunity looks a lot like hard work." - Ashton Kutcher
*donning my special humpday flame retardant suit*
Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752
___
Histo
t and well demarcated, and easily
distinguishable from the type seen in drying artifact.
Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752
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essary (no cross-linking occurred).
Your biggest problem may be that the antibody you are using does not recognize
an alcohol-fixed protein, and no amount of antigen retrieval can fix that.
Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
85
ern.edu/mailman/listinfo/histonet and change your mail
delivery.
Otherwise, I recommend a quick scan and liberal use of the delete button.
Teri Johnson
Manager, Histology
GNF - San Diego, CA
858-332-4752
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Bravo to Donna Emge for her detailed response on this issue. I am really
looking forward to seeing the YouTube videos you make. There seems to be a
critical lack of these available for histology technique!
Best wishes,
Teri Johnson
Manager, Histology
GNF - San Diego, CA
858-332-4752
nutes prior to staining. Might be worthwhile to try a different
retrieval method for these and see what happens.
Otherwise, are you sure you are using a clone that reacts in mouse tissue? We
use Rabbit monoclonal SP6.
Teri Johnson
Manager, Histology
GNF - San Diego, CA
858-332
For what it's worth, if I were to ever seriously consider moving, I'd snatch
this job up in a minute. I would work for Liz without reservation.
Teri Johnson
Manager, Histology
GNF - San Diego, CA
858-332-4752
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Teri:
The automatic coverslipper will wok on oven dried stained sections. I used them
on a Sakura film coverslipper and my lab was in Miami Beach, and you do not
more humid than that!
Xylene is the one weakening the immunoreactivity the most but I have tested the
IPA and the weakening does not
[mailto:rjbu...@yahoo.com]
Sent: Tuesday, August 13, 2013 8:41 AM
To: Teri Johnson; histonet@lists.utsouthwestern.edu
Subject: [Histonet] Isopropyl Alcohol in the histology lab
Teri:
It is a wise decision to substitute EthOL with Isopropyl alcohol (IPA) and to
your questions:
1- although it is not
who use IPA found any impact on immunoreactivity when
compared to using other dehydrants?
4 - Is there anything else I have overlooked?
We have pure ethanol available to us if needed for particular stain
applications.
Best wishes, and thanks in advance.
Teri Johnson
Manager, Histology
GNF -
bath? If it's low it can trap
an air bubble at the very top of the slide when it finishes dropping the
coverslip.
Teri Johnson
Manager, Histology
GNF - San Diego, CA
858-332-4752
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ng this email.
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Teri Johnson
[tjohn...@gnf.org]
Sent: Wednesday, 3 July 2013 3:24 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: H&E stai
t;
>
>
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145
>
> P Please consider the environment before printing this email.
>
>
> From: histonet-boun...@lists.utsouthwestern.edu
> [histon
periodic acid as an oxidation step before hematoxylin. It needs to be
rinsed well so there is no periodic acid carryover (that'll kill your
hematoxylin!), but that might improve your staining.
If you get this figured out, please let us know the cause and fix.
Teri Johnson
Manager, Histolog
this unit yet.
Feel free to email me directly.
Best wishes,
Teri Johnson
Manager, Histology
GNF - San Diego, CA
858-332-4752
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w they expect this to be done...
using specimens from his/her family members.
Teri Johnson
Manager, Histology
GNF - San Diego, CA
858-332-4752
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Dear colleagues,
If you are doing Immunofluorescence on the Ventana instruments (Alexafluor
labeling), please contact me. Bonus points if you are doing them on the
Discovery XT.
Teri Johnson
Manager, Histology
GNF - San Diego, CA
858-332-4752
NBF.
Silver stains may not stain as cleanly as NBF fixed tissues.
IHC retrieval may need to be altered from your SOPs.
Other than that, our hematopathologist loved it for lymph nodes and bone
marrows. It became the lab standard after getting rid of B-5 fixative.
Teri Johnson
Manager, Histology
will retire and just
veg.
I'm hoping Jim Burchette leads the way. There are fish to catch!
Best wishes to you in your new role,
Teri Johnson
Manager, Histology
GNF - San Diego, CA
858-332-4752
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the results of a freezing
bath over the cryostat peltier method.
Melanie S. White, MT(ASCP)
Laboratory Supervisor, Systems/Anatomic Pathology
McLeod Regional Medical Center
(843) 777-2072
Ditto what she said about the Novec fluid. It works just as well, without the
storage hazard.
Teri
make a difference
to any subsequent testing downstream. Make sure you use two sets of controls,
one with just regular depar, and one treated just like these slides.
Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Foundation
858-332-4752
Hi Jackie,
The ethanol we use is denatured with a lot of stuff that can include methanol,
MIBK, Toluene, and ethyl acetate , and our nuclear stains are crisp and sharp.
I would start looking at fixation first, followed by their H&E stain procedure
and reagents.
Teri Johnson, HT(ASCP)QIHC
This might or might not be helpful, but there was an article written by Janet
Maas, Processing the Complete Canine Tooth. It is for paraffin sectioning.
JOH, Number 3, Sept 2002, pp. 137-140(4)
Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research
different fixative
altogether. The protocol might need to be re-optimized for your fish samples
based on those issues.
Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Foundation
858-332-4752
___
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.
Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Foundation
858-332-4752
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ing? In my experience, the Accuedge
low profile blades tend to curl more quickly than other blades. You can try
using a thicker blade or one from a different manufacturer and see if that
helps.
Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Foundation
85
Message: 16
Date: Tue, 29 Jan 2013 09:35:16 -0800 (PST)
From: "Stephen Peters M.D."
Subject: [Histonet] hi
To: histonet@lists.utsouthwestern.edu
Message-ID:
<1359480916.92967.androidmob...@web163906.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=us-ascii
have a look at this (hyperl
need it.
Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Foundation
858-332-4752
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Dear Tim,
Here's the deal, they all work. When buying new, I bought the VIP5 due to its
great reputation. It was and still is a workhorse.
Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Foundation
858-332
could introduce
variation in your experimental outcome. Be very critical of the staining
results you obtain on these samples compared to your others.
Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Foundation
858-332-4752
We always put a small (tiny!) drop of Kodak PhotoFlo in the water bath. It's a
surfactant, so likely any diluted detergent would work. Be careful not to get
too much in there, your ribbons will want to sink.
Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Nov
your fluorescence and your chromogen technique
besides the H2O2? Fewer steps and washes? Make sure your buffer rinses are done
gently, maybe in a coplin jar instead of squirting with a squeeze bottle.
Good luck!
Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Rese
think that cutting temperature is too cold when your tissue sample shatters
when sectioning (depending too on the thickness). If you can rub your thumb
across it and you get a few decent sections, the warming of the specimen likely
explains it.
Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab
g it hasn't as it might inadvertently
promote the status quo.
Teri Johnson HT(ASCP),QIHC
Disclaimer: The thoughts conveyed above are strictly my own and do not reflect
in any way on my employer, co-workers, family members, deceased pets, and
future ex-husbands.
___
et of experienced techs who do not have these degrees or certifications who
are highly qualified for the position and would face elimination from
consideration based on that alone. You can list it as highly desirable instead
of a requirement.
Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Gen
ibodies to find one that would work under those conditions?
The only way to know if heat or other conditions will negatively affect your
target protein is to test it empirically.
Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Foundation
85
I have seen a couple emails where people who have the capability of dewaxing on
the immunostainer but instead do the dewaxing offline. I am curious as to why
you choose to do this.
Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Foundation
858
fungus in the
tissue. Some labs moved to fast green as a counterstain instead because of this
issue.
Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Foundation
858-332-4752
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Histonet mailing list
er adopted this practice but would love to see data that shows it is better
in some circumstances. I think the practice does not hurt anything but falls
under the heading "just in case." Gayle Callis, among others, may be able to
shed some additional light on this.
Happy Friday!
Teri
then cryoprotecting
with sucrose prior to freezing. It appears they recommend using a biotinylated
secondary and then streptavidin-HRP for staining. You might see if that helps
as well.
Teri
Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Found
Dear Adam,
Good luck finding it. There are some thick watch glasses that might work for
your purposes but they aren't the size of glass slides. EMS has some well
dishes that also might work, but they are also bigger.
http://www.carolina.com/category/equipment+and+supplies/glass+and+plasticware/
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