Hi Blanca,
Hiring has become more difficult in recent years. Most employers now only
verify past employment. They cannot divulge any corrective actions, performance
issues or firings.
References are hand-picked by the applicant so you don't get the whole picture
there either.
I typically ask
I'm also a fan of having any fellow employees available ask questions. I
have my staff limit it to histology related subjects, but my thought is if
they are going to be the ones working directly with them, it helps to have
their opinions.
Patrick Laurie(HT)ASCP QIHC
Histology Manager
Celligent
I'm not sure if this is an option, but we used to melt down old blocks and make
the HT embed and cut them. This showed us how good and quick they were and if
they actually new what they were doing. Anne
-Original Message-
From: Blanca Lopez via Histonet
Sent: Thursday, September 12,
Hi Blanca,
I think practical skills tests are a great idea. BUT I also think you need
to make sure that having someone that isn't an employee doing tasks in the
lab is a responsibility your lab would willingly take on if something were
to happen. If the candidate cut themselves and was litigious
On Date: Mon, 23 Jul 2018 18:31:48 -0400
From: Mary Ann
Subject: [Histonet] Positive PAP
Mary Ann wrote "Help! My pathologist has asked that a positive patient be run
down with our PAP stain for QC.
Point me to a reference to counter this request."
Hi Mary Ann - First of all, my sympathies.
Jenn,
Trying to fully understand. You need someone to prepare these slides again
for you? Great quality work, you provide the specimen? Let me know, I
would love to learn more.
Sincerely,
JB
On Wed, May 31, 2017, 9:13 AM Dearolf, Jenn via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
: Re: [Histonet] help!!
Tim and Tony,
Why couldn't DAB be used on frozen sections in your example?
Allan
On Mon, Apr 17, 2017 at 9:03 PM, Tony Henwood (SCHN) via Histonet
<histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>>
wrote:
Hi Bianca,
Well for most Patho
Tim and Tony,
Why couldn't DAB be used on frozen sections in your example?
Allan
On Mon, Apr 17, 2017 at 9:03 PM, Tony Henwood (SCHN) via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Hi Bianca,
> Well for most Pathology departments, Immunofluorescence (IF) is used for
> Renal and
Hi Bianca,
Well for most Pathology departments, Immunofluorescence (IF) is used for Renal
and Skin biopsies; looking for human Immunoglobulin (Igs) deposition on
basement membranes. The advantage here is not so much the fluorescence, but
that we use unfixed frozen sections. The buffer rinse
Blanca,
Here are my feelings on this, and I am sure a lot of other folks have feels
here too, so please chime in.
1 - I feel that most clinical labs are more on the IHC bandwagon and
research labs are IF (with the exception of IgG staining in kidney biopsies
or bullous disease in skin- which is
Blanca, immunofluorescence (IF) is a subset of immunochemistry.
Immunohistochemistry is also a subset of immunochemistry. There is some overlap
between the two.
Immunohistochemistry denotes immunochemistry done on tissue sections
("-histo-" =" tissue"). But we can also use other enzymes to
mailto:histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Help with causes or theory behind failure of nuclear
counterstaining after IHC!
Maria,
If the counterstain is good when done before IHC stain and poor after it sounds
like proteins are being extracted during the IHC processing
Maria,
If the counterstain is good when done before IHC stain and poor after it sounds
like proteins are being extracted during the IHC processing and staining. Have
you tried staining sections after each step of the IHC process to isolate the
point the stain becomes weak?
Tim Morken
I'd be very interested in this as well...could you shaere Karen.
Thanks.
C
On Mon, Nov 2, 2015 at 11:43 AM, Karen Cai via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> HELP:
>
>
>
> Hi,
>
> Is there anybody can provide me the price list/structure of the custom IHC
> services?
>
>
>
--
*From:* Patsy Ruegg [prueg...@hotmail.com]
*Sent:* Sunday, January 04, 2015 7:03 PM
*To:* Jay Lundgren; Maria Mejia
*Cc:* Histonet@Lists. Edu; Mejia, Mary
*Subject:* RE: [Histonet] HELP! Need some old fashioned histology advice
I have done something similar
, January 04, 2015 7:03 PM
To: Jay Lundgren; Maria Mejia
Cc: Histonet@Lists. Edu; Mejia, Mary
Subject: RE: [Histonet] HELP! Need some old fashioned histology advice
I have done something similar to this but I used tissue that was fixed but not
processed and embedded, this is called enblock labeling, I
I can help with the old fashioned advice:
- 1 scant teaspoon simple syrup
- 2 dashes Angostura Bitters, plus more to taste
- 1 half dollar–sized slice orange peel, including pith
- 2 ounces good-quality rye or bourbon
- 1 maraschino cherry
As for the Histology, is there any
: Sun, 4 Jan 2015 11:58:38 -0600
From: jaylundg...@gmail.com
To: mbmph...@gmail.com
Subject: Re: [Histonet] HELP! Need some old fashioned histology advice
CC: histonet@lists.utsouthwestern.edu; mary.me...@ucsf.edu
I can help with the old fashioned advice:
- 1 scant teaspoon simple syrup
Hans
We use the antibody from Serotec, it works quite nicely. MCA711, proteinase K
digestion, rabbit anti-rat secondary and then Envision Rabbit (polymer). We
use this antibody at a 1:1200 dilution.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder,
Of Elizabeth
Chlipala
Sent: Thursday, November 06, 2014 12:41 PM
To: Hans B Snyder; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] HELP
Hans
We use the antibody from Serotec, it works quite nicely. MCA711, proteinase K
digestion, rabbit anti-rat secondary and then Envision Rabbit
Thanks Sandra! My next step was to try that.
Dakshna
- Original Message -
From: Sandra E. Esparza sespa...@seton.org
To: Dakshnapriya Balasubbramanian dakshnapr...@neo.tamu.edu
Sent: Tuesday, May 20, 2014 10:34:25 AM
Subject: RE: [Histonet] HELP- Cryosectioning FAT!
You might want
To: Dakshnapriya Balasubbramanian dakshnapr...@neo.tamu.edu
Sent: Tuesday, May 20, 2014 10:37:35 AM
Subject: RE: [Histonet] HELP- Cryosectioning FAT!
Dakshna,
Has this tissue been fixed before freezing by any chance?
laurie
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Hi Rui,
You uploaded a tif image and a number of browsers don't support tif
images. Jpeg, gif and png images are the best image formats to use
because they are universally supported. I converted your image to a jpeg
image and posted it at:
: Re: [Histonet] Help for identifying the blue stained structure
Hi Rui,
You uploaded a tif image and a number of browsers don't support tif
images. Jpeg, gif and png images are the best image formats to use
because they are universally supported. I converted
Thanks very much Jean. I will call NSH office. Dorothy
On Fri, Jan 17, 2014 at 2:53 PM, Mitchell Jean A jmitch...@uwhealth.orgwrote:
Dorothy: I know that there have been some issues with Maney Online
lately. I suggest your contact the NSH office and they should be able to
assist you. I
FACEPALM.JPG
this is not even worth an actual jpg.
sigh.
By bitching and bitching and bitching, they could exhaust the drama of
their own horror stories. Grow bored. Only then could they accept a new
story for their lives. Move forward.
-Chuck Palahniuk, Haunted
On Wed, Sep 11, 2013 at 7:51
Googling 'unsubscribe histonet' points me to this link:
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
If you scroll to the bottom of the page you will find a box where you can
type your e-mail address to unsubscribe or edit options. Have you tried
that?
Cheers.
--
Virginia Bain
lol
On Wed, Sep 11, 2013 at 5:56 PM, Bain,Virginia veb...@mdanderson.orgwrote:
Googling 'unsubscribe histonet' points me to this link:
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
If you scroll to the bottom of the page you will find a box where you can
type your e-mail
Yes, it is for review of material for molecular testing and can be added at any
time as long as it is not ordered at the time the case is in process. If it is
more than 30 days after the patient has been discharged, it is considered
archived (according to Medicare) and we register it for a new
It can't be used to just pull blocks. The slides have to be reviewed and
the best block chosen by a pathologist. If there is only one block then
the pathologist needs to look at the slides and determine if there is
enough tissue for molecular testing. It's a professional charge.
Use it on
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango
Sent: Thursday, January 10, 2013 2:01 PM
To: Natalie Nagy
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Help with CPT code 88363 for archived tissue retrieval
It can't be used to just pull blocks
: Re: [Histonet] Help with CPT code 88363 for archived tissue retrieval
It can't be used to just pull blocks. The slides have to be reviewed and the
best block chosen by a pathologist. If there is only one block then the
pathologist needs to look at the slides and determine if there is enough
PM
To: 'Mark Tarango'; Natalie Nagy
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Help with CPT code 88363 for archived tissue
retrieval
And I should explain the reason I most know this.. our pathologists were
denied because the tech charge hadn't been entered yet. So now I make
@lists.utsouthwestern.edu
Subject: RE: [Histonet] Help with CPT code 88363 for archived tissue retrieval
So are you putting the charge thru twice or is the charge for the 88363 divided
out into tech and prof.?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun
...@lists.utsouthwestern.edu] On Behalf Of Weems,
Joyce K.
Sent: Thursday, January 10, 2013 1:06 PM
To: 'Mark Tarango'; Natalie Nagy
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Help with CPT code 88363 for archived tissue
retrieval
And I should explain the reason I most know this.. our pathologists
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun
Sent: Thursday, January 10, 2013 2:47 PM
To: Joyce K. Weems; Mark Tarango; Mike Pence; Natalie Nagy
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Help with CPT code 88363 for archived
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Thursday, January 10, 2013 1:06 PM
To: 'Mark Tarango'; Natalie Nagy
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Help with CPT code 88363 for archived tissue retrieval
And I should
To: 'Bell, Lynne'; 'Richard Cartun'; Weems, Joyce K.; 'Mark Tarango'; 'Mike
Pence'; 'Natalie Nagy'
Cc: 'histonet@lists.utsouthwestern.edu'
Subject: RE: [Histonet] Help with CPT code 88363 for archived tissue retrieval
We have been billing a tech and pro fee since 2011. Whether we are getting
reimbursed
Hi Ly,
When you click on the Unsubscribe button, an email is sent to you to
confirm you want to unsubscribe. It has a link to click to confirm you
want to unsubscribe. Is the confirmation email getting lost in your junk
folder? You won't be unsubscribed until you click the link in the
: [Histonet] Help
Hi Ly,
When you click on the Unsubscribe button, an email is sent to you to confirm
you want to unsubscribe. It has a link to click to confirm you want to
unsubscribe. Is the confirmation email getting lost in your junk folder? You
won't be unsubscribed until you click the link
] On Behalf Of Marvin Hanna
Sent: Friday, December 28, 2012 2:28 PM
To: histonet@lists.utsouthwestern.edu; ln0...@gmail.com
Subject: Re: [Histonet] Help
Hi Ly,
When you click on the Unsubscribe button, an email is sent to you to confirm you want to
unsubscribe. It has a link to click to confirm
: [Histonet] Help! Liver mistakenly processed in paraffin (had
tobe in OCT instead)!
It depends on what you are using the oil red o for. Lipofuscin and ceroid
can be demonstrated with an oil red o stain after processing.
Jennifer MacDonald
From: z o n k e d zon...@gmail.com
To: histonet
Nope, sorry. All your fat is dissolved.
Sent from my iPhone
On Nov 13, 2012, at 8:52 AM, z o n k e d zon...@gmail.com wrote:
Hello Histonetters,
First time writer, long time reader. I'm a newbie tech in academia and I
was given a simple task which I think I pretty much screwed up.
I
Oil Red O is a stain for fat, Alcohol dissolves fat Rehydrating won't
help. You can't replace the fat.
Rena Fail
On Tue, Nov 13, 2012 at 11:52 AM, z o n k e d zon...@gmail.com wrote:
Hello Histonetters,
First time writer, long time reader. I'm a newbie tech in academia and I
was given a
It depends on what you are using the oil red o for. Lipofuscin and ceroid
can be demonstrated with an oil red o stain after processing.
Jennifer MacDonald
From: z o n k e d zon...@gmail.com
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Date: 11/13/2012
You really screwed it up!
When you placed both pieces of liver in the processor both were subjected to
the effect of ethanol and probably xylene and both reagents extracted the liver
fat and no matter what you try to do now, there will be not enough fat in the
pieces as to even try the ORO
Do you mean to try to demonstrate if the fungi you find in a sample WAS dead or
alive before it was killed during the fixation and processing?
My take on this is the following: if a fungi dies naturally in a tissue
(either lung or nail, or whatever) that dead fungi either is decayed and lost
or
What you describe is a typical example of poor paraffin infiltration = the
paraffin has not infiltrated the tissue and when you prepare the final block it
will consist of 2 different components; the tissue and the paraffin. That is
why you end with a good paraffin section without the tissue.
In addition to Rene's comment,to cut coagulated tissue (skin that have
new wound crust) and calcified tissue is difficult.
On Wed, Aug 8, 2012 at 6:45 AM, Megha Kumar meg...@g.clemson.edu wrote:
Hi All
I am trying to section adult mouse intestine and skin using paraffin
embedding.
Nancy,
We've had similar issues with fatty tissue falling off of the slides
while performing IHC. We use Superfrost + slides which we have found
to really hold the tissue well. Also, I have learned through reading
round on the Histonet that air drying doesn't completely remove the
water from
I realize that such + slides come with the instruction to completely
dry slides at room temperature before placing in the drying oven. I
have used these slides for many years, and have found this procedure to
be not only unnecessary, but sometimes problematic. I believe sections
are more likely
As to your issue of tissue not adhering to the slides, you could try to check
the expiration date of your (+) slides. Perhaps it is just an issue with the
slide.
As to controlling the concentration of an antibody by changing the incubation
time, that is somewhat unorthodox to say the least. You
Hi Lydia,
I think Tony Henwood has it exactly right in talking of DAB intensification.
The article he sites and several others show how much more sensitive DAB
intensification can make an ordinary iron reaction. And you are not looking in
bone marrow or spleen but somewhere where there
The Turnbull's blue method as you describe it will not detect iron in tissues.
Potassium ferricyanide will give a blue precipitate only with iron(II)
(ferrous). In tissues, the iron is present as iron(III) (ferric) in such
proteins as ferritin and haemosiderin. The iron of
Jennifer,
Looking at the images, is it possible that the slides were air-dried for too
long prior to staining?
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at
We do this stain on frozen sections in our lab with no formalin fixation. We
stain in oil red o from American Mastertech for 30 minutes, rinse in water,
counterstain in hematoxylin, coverslip with aqueous mounting media.
Lisa
-Original Message-
From:
We use pre-fixed tissue for oil red o. We rinse the section briefly to
remove the formalin from the outside of the tissue. We prepare our tissue
in OCT or the equivalent. We cut the frozen sections and mount them on
charged slides. We let dry the dry a minimum of 10 minute, rinse them in
We usually run them on our automated stainers but I have been known to do them
by hand in a pinch
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From:
Our problem, in my opinion is a control issue and not a staining one..our
pathologist wants to see the bugs in certain areas-glands(spelling
appologies!) its called, the circular groups of cells, in the middle of
these areas and not just occasional bugs in other areasEverywhere else
I've
From: Jay Lundgren [jaylundg...@gmail.com]
Sent: Tuesday, March 01, 2011 3:10 PM
To: McMahon, Loralee A
Cc: Heather Cooper; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] HELP! H. Pylori Immunos
Our problem, in my opinion is a control issue and not a staining one..our
Donna
I have seen soft or rather sticky OCT samples in the past. If you
freeze in isopentane and don't let the isopentane evaporate off the
samples prior to wrapping the sample in foil, that excess isopentane
changes the OCT, it makes it sticky.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Donna,
I would also check to make sure that the tissue wasn't held in an
something with a lot of alcohol, like RNA later. I found out first
hand that it doesn't work well. Good luck
On Thu, Feb 10, 2011 at 11:38 AM, Reynolds,Donna M
dreyn...@mdanderson.org wrote:
Has anyone ever experienced
I agree with previous responders to this query, as I've gotten alcohol on
my blocks and since alcohol doesn't freeze real well it makes a sticky mess
with the OCT. The affected OCT can be scraped off with a razor blade.
Hopefully the tissue itself did not come in contact with alcohol prior to
-boun...@lists.utsouthwestern.edu on behalf of Liz Chlipala
Sent: Thu 2/10/2011 1:49 PM
To: Reynolds,Donna M; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Help with OCT problem
Donna
I have seen soft or rather sticky OCT samples in the past. If you
freeze in isopentane and don't let
Kathy-
What do these cracks look like? Are they arranged in a parallel manner? Or do
they have the appearance of the cracks seen in dry mud?
Parallel aligned cracks are often found in overprocessed small biospies. These
small samples become hard and brittle. The impact of the tissue on the
Thanks, Montina
The sections are 40um and I had no trouble with all my other brains
(sectioned myself). I had help with these last two and they are all curled.
I think they just got sectioned too fast or something. I'll try the shaker
and see if it helps.
Thanks!
-Teresa
On Wed, Sep 15, 2010 at
] On Behalf Of connie grubaugh
Sent: Tuesday, June 22, 2010 9:06 PM
To: cg...@marylandgeneral.org; jcbrit...@cheshire-med.com; dianar...@aol.com;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Help! In need of positive Gram Control
Tried the slim jim and all of my doctors did not like
@lists.utsouthwestern.edu
Subject: RE: [Histonet] Help! In need of positive Gram Control
You have got to be kidding!! That's hysterical. So process a slim jim
and you have
Gram - and + controls. If you're serious I'm trying it.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
We have success with Sandison Method, see below:
Sandison Method
Solutions:
95% ethanol 300ml
38% Formalin10ml
Sodium carbonate10g
Water 690ml
Method:
Leave tissues in solution overnight or until they become soft.
Sent: Tuesday, June 22, 2010 6:10 AM
To: dianar...@aol.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Help! In need of positive Gram Control
Have you tried a Slim Jim? They have gram positive and negative rods in
them. Regardless, I still enjoy eating them once and a while
@lists.utsouthwestern.edu
Subject: RE: [Histonet] Help! In need of positive Gram Control
Have you tried a Slim Jim? They have gram positive and negative rods in
them. Regardless, I still enjoy eating them once and a while!
Josie Britton Ht
Cheshire Medical Center
Keene, NH 03431
A good ol' hot appendix works great. Not as good as a Slim Jim, tho.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Tried the slim jim and all of my doctors did not like it. Don't waste your
time.
Connie G.
Date: Tue, 22 Jun 2010 10:16:14 -0400
From: cg...@marylandgeneral.org
To: jcbrit...@cheshire-med.com; dianar...@aol.com;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Help
Go to your local drive in mart and buy you a Slim Jim, cut it in thin slices
and you will have all the Gram controls you can use. You will never eat
another Slim Jim. If you don't know what they are, they are sort of like a
summer sausage, only thinner and guys love them. They are found
I think there is none... But thanks for the funny!! j
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Tuesday, March 23, 2010 12:00
To: histonet@lists.utsouthwestern.edu
Subject:
So sorry... the only thing I know to fix it is time... about a week or
two... :)
Drew
On Tue, Mar 23, 2010 at 11:59, Patsy Ruegg pru...@ihctech.net wrote:
After you stop laughing seriously I need some help here, apparently I got
my
fingers in some silver nitrate yesterday and touched my face
You could use a sharpie to fill in a killer moustache.
I say go with handlebars and not with Hitler-style.
Emily
Shall we always be content with the ancient tinned salad of the subsidized
novel? Or the tired ice-cream of poems which cry themselves to sleep in the
refrigerators of the mind?
...@ihctech.net; histonet@lists.utsouthwestern.edu
Sent: Tue, March 23, 2010 11:09:35 AM
Subject: Re: [Histonet] help
You could use a sharpie to fill in a killer moustache.
I say go with handlebars and not with Hitler-style.
Emily
Shall we always be content with the ancient tinned salad of the subsidized
Suggest you Google Henna tattoos and then make a feature of it with a
few tasteful scrolls and patterns.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: 23 March 2010 16:00
To:
Basically time
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Tuesday, March 23, 2010 11:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] help
After you stop laughing
Classification: UNCLASSIFIED
Caveats: NONE
Patsy,
I have removed Silver Nitrate on my hands by first treating the stain with
Iodine solution then flushing the area with Sodium Thiosulfate solution (5% ?)
then washing my hands thoroughly with soap and water. It removed the Silver
Nitrate.
are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.
-Original Message-
From: Weems, Joyce [mailto:jwe...@sjha.org]
Sent: Tuesday, March 23, 2010 10:08 AM
To: Patsy Ruegg; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] help
I think
Pretty funny stuff!! I say to try the sodium thio - it removes unreduced
silver. Or you could grab a Brillo pad and scrub away!
This laugh was just what I needed today. Thank you.
Lynne A. Bell, HT (ASCP)
Technical Specialist, Histology
Central Vermont Medical Center
130 Fisher Road
Barre,
Patsy:
Chemically, silver nitrate deposites can be
reduced onto the slides by 0.5% potassium ferricyanide.
I am not sure that it may work onto living skin.
Sincerely,
Maxim Peshkov,
Russia,
Taganrog.
mailto:maxim...@mail.ru
___
: Maxim Peshkov [mailto:maxim...@mail.ru]
Sent: Tuesday, March 23, 2010 11:43 AM
To: pru...@ihctech.net
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] help (UNCLASSIFIED)
Patsy:
Chemically, silver nitrate deposites can be
reduced onto the slides by 0.5% potassium ferricyanide.
I am
Some 35 years ago, a person older than I told me the most effective way to get
silver stains off skin was to rub the affected part with slightly dampened
mercuric chloride powder. He may have been right; I never tried it! I've tried
things like iodine-thiosulphate and Farmer's reducer
Touch the affected areas with Lugol's solution. After that remove the Lugol
with sodium thiosulfate.
René J.
--- On Tue, 3/23/10, Patsy Ruegg pru...@ihctech.net wrote:
From: Patsy Ruegg pru...@ihctech.net
Subject: [Histonet] help
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, March 23,
@lists.utsouthwestern.edu
Subject
RE: [Histonet] help (UNCLASSIFIED)
Are you trying to kill me?
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pru...@ihctech.net
web site www.ihctech.net
This email
Fawn
CAP has specific quidelines, its right there in the questions, did you
do what that question is asking and have you documented what was done.
They tell you right in the check sheet what needs to be completed. Test
validation must be performed on a minimum of 25 cases (they recommend
25-
And what did you do?
René J.
--- On Thu, 2/11/10, Fawn Bomar fawn.bo...@halifaxregional.com wrote:
From: Fawn Bomar fawn.bo...@halifaxregional.com
Subject: [Histonet] Help...
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date: Thursday, February 11, 2010, 2:06 PM
I would suggest you check the lot#. You may have received a bad lot and need to
get them replaced by the vendor.
Hi all,
We have suddenly started losing tissue sections from our Superfrost Plus
Slides.
Our students have been cutting fixed, frozen, cryosections (20um) and
Pretty good list.
Don't forget first aid kits and spill kits.
Jerry Ricks
Research Scientist
University of Washington
Department of Pathology
Date: Mon, 20 Jul 2009 14:26:57 -0400
From: ktut...@umm.edu
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HELP Starting a lab
I have
@lists.utsouthwestern.edu; sr...@aol.com
Subject: Re: [Histonet] Help With Hemo Fading
The fading most probably is caused by acid in the permanent slide, probably
because the sections were passed through the alcohols very quickly after the
acid differentiation, or they stayed little time in tap water
To: histonet@lists.utsouthwestern.edu; sr...@aol.com
Subject: Re: [Histonet] Help With Hemo Fading
The fading most probably is caused by acid in the permanent slide, probably
because the sections were passed through the alcohols very quickly after the
acid differentiation, or they stayed
The fading most probably is caused by acid in the permanent slide, probably
because the sections were passed through the alcohols very quickly after the
acid differentiation, or they stayed little time in tap water after
differentiation or no bluing agent was used.
It is unlikely that the
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Rene J Buesa
Sent: Thu 7/2/2009 3:27 PM
To: histonet@lists.utsouthwestern.edu; sr...@aol.com
Subject: Re: [Histonet] Help With Hemo Fading
The fading most probably is caused by acid in the permanent slide
Leroy,
You could also have a electronics technician troubleshoot and repair your
screens. You know. Transistors, diodes, triacs, capacitors which are all
available online. Hardly anyone even thinks of that option anymore. Let me
know if I can help you.
Paul
Paul M. Firnschild
QA Support
Your advise to her is correct: probably the sections are not destained
completely.
Any section stained with the Ziehl-Nielsen Carbol Fucsin has to be treated with
acid alcohol until no more red color leaches from the section no matter how
much time that takes. No more red coming out of the
If the CC1 standard 32 with amp, 42 degrees does not work then try using
protease 1 4-6 minutes on it.
On Fri, Nov 28, 2008 at 12:35 PM, Lena Spencer [EMAIL PROTECTED]wrote:
Hi All:
I have been working up the PMS2 and MLH2 mismatched repair genes and I am
not satisfied with my results. I am
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