I cannot believe any scientist would advocate such a non-uniform method
as spitting on a slide.
Buy a bottle of what ever enzyme and use a reproducible buffer and
temperature.
Geoff
On 5/5/2016 3:19 PM, Anne Murvosh via Histonet wrote:
Yes, spitting is the tried and true way to do
I automatically delete all of his posts.
Geoff
On 4/14/2016 9:01 AM, Rene J Buesa via Histonet wrote:
Amen!!!But you have to concede that Lester is a very persistent, almost obstinate
individual, probably used to impose his will and this postings are just an example of it:
he likes his blog
All of my cameras are operated digitally.
Geoff
On 7/14/2015 1:50 PM, Monson, Frederick via Histonet wrote:
A strongly recommended first interview question: How many digital cameras do you
have with you today?
Cheers,
FCM
Frederick C. Monson, PhD
Technical Director
Center
Why would you want to do this?
You could buy plain slides and sub them yourself.
Geoff
On 7/9/2015 8:45 AM, Travers, Susan wrote:
Does anyone know if it's ok to sub positively charged slides with chrome
alum-gelatin. I seem to remember an incident where this did not work- but maybe
it's just
Huh?
Take a solution more dilute than you want it and dilute it more?
Geoff
On 5/1/2015 10:41 AM, Goins, Tresa wrote:
All that matters here is the final concentration of the reagent - it doesn't
matter what stock you start with if you calculate the dilution.
Adding 1.25 ml to 1000 ml
Failure to completely remove paraffin is often a cause of uneven
staining. Change all of the xylenes and add another xylene.
Geoff
On 4/1/2015 12:06 PM, Julie Cohen wrote:
Hi,
I made slides of paraffin-embedded mouse small intestine (Swiss rolls), and
stained them with Hematoxylin and Eosin
You can read it here:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768083/
Geoff
On 2/18/2015 2:48 PM, Fawn Bomar wrote:
Hi all,
I need some help in locating an article for class, preferably one I don't have to pay
for. The name of the article is The Effectiveness of Honey
My error, I sent a link to a similar article. The original is in The
Journal of Histotechnology. 2006;29(3):173–176 but my library does not
have that journal. Perhaps someone on the list has it.
Geoff
On 2/18/2015 2:48 PM, Fawn Bomar wrote:
Hi all,
I need some help in locating an article
This is common with mouse and rat tissues, they get over-dried with a
typical processing schedule.
Soaking the face of the block with a kimwipe wet with ice water for 60
-120 seconds will enable you to cut 10 nice sections, maybe more.
Geoff
On 2/5/2015 6:23 AM, Emily Brown wrote:
Hello all
From the point of view of anatomy of the wrist and potential for stress
on the tendons in the carpal tunnel, gotta be #2.
Geoff
On 12/8/2014 3:53 PM, Smith, Denise wrote:
Hi all!
When I took HTL exam and there was a question on the exam that bothered me the
most but I cannot find an exact
Assuming that the 'crust' is a precipitate, what is it soluble in?
Water, alcohol, dilute acid or base?
It shows up if you just run the peroxide+DAB rxn?
Geoff
On 9/19/2014 8:14 AM, Donna Maney wrote:
Hello,
I've been doing IHC on free-floating brain sections for about 15 years using
Teri makes an excellent point about higher alcohols causing
precipitation of phosphate salts.
Geoff
On 9/22/2014 1:17 PM, Teri Johnson wrote:
Hi Donna,
Interesting conundrum! A few questions:
- Do you see it in tissues that have not undergone any staining, just floated
in buffer
Greetings Histoland:
I have an old AO 860 sliding microtome that needs cleaning and
lubrication, it skips sections.
Anyone have a service manual, either paper or PDF, they can share?
Advice would work too.
Thanks in advance.
Geoff
--
--
**
Geoff
Lowe's or Home Depot. They don't say science on the side but who cares?
Geoff
On 4/30/2013 10:38 AM, Shirley A. Powell wrote:
You can also check with restaurant supply houses, we have purchased great large
containers with lids for this purpose. Much cheaper than the lab supply houses
In our library all of these are still known as /Stain Technology/. The
name change to /Biotechnic and Histochemistry/ happened much later.
Geoff
On 4/17/2013 9:14 PM, Tony Henwood (SCHN) wrote:
Some references that might be useful:
Paget, G. E., Eccleston, E. (1960). Simultaneous specific
It depends on the antigen. I know that sounds like a cop out but it is
true. Review the literature covering the antigen you are looking for to
see what has worked for others
Geoff
On 4/11/2013 6:19 AM, Ian R Bernard wrote:
Pretreatments are used to recover bonded antigen sites owing
Rene is correct, formalin fixes slowly so more time is needed. Raising
the concentration of formaldehyde will not help. Adding a small amount
of glutaraldehyde (0.25 to 0.5%) will fix more quickly but may have a
negative effect on immunostaining.
Geoff
On 3/13/2013 6:31 AM, Giulia Zunino
you in advanced,
Louisa M Papke
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Perfusion with what? Fixation for how long? What is your processing
schedule?
Geoff
, they must be completely frozen very quickly, then
allowed to equilibrate in the cryostat.
-20C should be fine, is your knife sharp?
Geoff
On 1/23/2013 12:54 PM, Papke, Louisa M. wrote:
The mice were perfused with 4% PFA. Then left in 4% PFA for about 2 weeks.
The processing schedule
What issues are you having? That might help the list diagnose the problem.
Geoff
On 12/6/2012 3:43 PM, Andrew Coleman wrote:
Hi all,
We are performing transcardial perfusions in rats using paraformaldehyde in
0.1M potassium phosphate buffer.
Can anyone think of any issues that would
I agree with Rene.
Geoff
On 12/3/2012 12:00 AM, Daniela Bodemer wrote:
Hi all,
Our tissue processor has been shut down due to a contamination issue and
now all the tissues (mice and rat pelves) collected prior to this
happening have been sitting in 4% PFA. Some tissues more than a week
I agree with Rene, Barry, etc.
Geoff
On 11/3/2012 2:41 PM, Gudrun Lang wrote:
Hi histonetters!
I'm just attending a histo-course, where the teacher told us his opinion
about overfixation.
For him overfixation takes place in any formaldehyde solution with a
concentration above 5%. This should
, making osmium for EM, etc.
Look up 'water break test' and see if you can find anything helpful.
Geoff
On 10/12/2012 11:40 AM, Valerie O'Connor wrote:
Hi All,
I process and stain GI biopsies only. I use do a basic HE, Diff Quick for
H.pylori, PAS for candida , Trichrome, and Alcian Blue/PAS
, packed
with explosives and BOOM! Of course it exploded, it was surrounded with
explosives.
Geoff
On 9/14/2012 8:58 PM, E. Wayne Johnson wrote:
What danger of Picric Acid are you concerned with?
Surely its not the hyped explosion hazards.
We use picric acid and as inquisitive boys we have tried
Fix for a week or two, then rinse out most of the picrates with 50-70%
ethanol. Fixation preserves cell morphology, it does not distort it.
Geoff
On 9/14/2012 9:06 AM, Margaret Horne wrote:
Hello Everyone, I am asking this for a friend.
How long can mouse testis be kept in Bouins without
in Bouin's is probably better than 1-2 days.
Long term months-years in rarely a good idea, why not just embed the
tissue?
Geoff
On 9/14/2012 9:58 AM, Margaret Horne wrote:
Thanks for the incoming info; it's a big help. Sorry I need a little bit
more detail so to explain a little more:
Some
Two other arguments against osmium, it is very expensive and is must be
disposed of as hazardous waste.
Geoff
On 8/24/2012 10:50 AM, Sheila Adey wrote:
Thank you so much for this response. :)
Subject: RE: [Histonet] Osmium tetroxide staining for lipids
Date: Fri, 24 Aug 2012 08:42:34
Add a few drops of glacial acetic acid.
Geoff
On 8/9/2012 11:23 AM, Hannen, Valerie wrote:
Hi Folks...I am hoping you all give me a little help. Our Pathologists are
complaining about our Eosin on the HE's being weak. The funny thing is, is
that it can go one
day to the next...one day
Wanda makes an excellent point, thinner sections have less contrast.
Geoff
On 8/9/2012 2:50 PM, wanda.sm...@hcahealthcare.com wrote:
How about the thickness of the tissue? Once we were cutting skins at 3 microns
and the pathologist complained the Eosin was too light so we started cutting
buffer for EM (that shows you how long ago this was!). So
I found the exact recipe but with NaOH. The techs all knew it was a typo
but she did not.
Geoff
On 7/11/2012 12:12 PM, Morken, Timothy wrote:
It's funny how histology lore gets passed down, passed around and misunderstood
over
in borax.
You might look at this paper by Charlotte Pool:
/Pool, C.R. Hematoxylin-eosin staining of OsO4-fixed epon embedded
tissue; prestaining oxidation by acidified H2O2. Stain Technol.
44:75-79, 1969./
Geoff
On 6/25/2012 9:55 AM, Shanon Pink wrote:
Hi Again Gayle,
I'm sorry I
On 6/7/2012 12:24 PM, nmhi...@comcast.net wrote:
This is a very logical way of determining who can follow directions...
IF they could follow directions ...
Geoff
- Original Message -
From: Sharon Beckhams...@stowers.org
To: Bridget' 'Maryottbridget.mary...@ventana.roche.com
up costs and unnecessary
surgery and related complications.
I can send the papers from NEJM if you like.
Geoff
And for all those advocating closure of private labs, do you also feel the same
way about private pathologist owned labs who reep the benefits of getting all
the out PT work from
and coverslip.
Source:_Staining Procedures_, 4th edition., George Clark editor.
Geoff
On 3/28/2012 10:56 AM, Amanda Madden wrote:
Good Morning Histonetters!
I am looking for advice on my thionin staining recipe and protocol. The
recipe I use currently ends up being just under 1% Thionin
1. Try an alcoholic fixative like formalin-alcohol-acetic acid
2. Try putting Safranin O in the fixative.
Shepard and Mitchell, J. Ultrastruc. Res. 54:451-460, 1976.
or
same authors used Toluidine Blue O
J. Histochem. Cytochem. 24:621-629, 1976.
Geoff
On 3/15/2012 8:41 PM, Jennifer Scholler
I agree with Rene. A control (undigested) slide is necessary.
Geoff
On 3/15/2012 1:47 AM, Jennifer MacDonald wrote:
Hi All,
At a local lab when a pathologist orders a PAS diastase the
histotechnicians do just one slide with diastase. They do not do an
undigested slide. How would
glove.
Not wearing the appropriate glove is almost certainly a safety violation.
Geoff
On 2/21/2012 10:17 PM, Jenny Vega wrote:
I am asking this because in my job we mount slides by hand, and my
coworkers don't like to use gloves because it leaves a residue of latex in
the back of the slides. I
Sigma Chemical, catalog number A3648
Geoff
On 2/14/2012 11:18 AM, Joseph Madary wrote:
Nick Madary, HT/HTL(ASCP)QIHC
George Washington University
Pathology Core Laboratory
Ross Hall, Room 706
23rd and I Street NW
Washington D.C. 20037
202.994.8916
pat...@gwumc.edu
I have some Grubler dyes in the original containers. Probably pre-WWII
but not dated.
Geoff
On 2/2/2012 4:14 PM, Rene J Buesa wrote:
Ha.Ha,Ha!!!
I used to prepare staining solutions with some Merck-Darmstad anilines manufactured
just after the Great War, i.e. the FIRST World War
in which the bomb squad packs explosives around
the picric acid (from an old high school lab) out in a field and sets it
off. BOOM! Sure, the picric acid was surrounded by explosives.
Geoff
On 2/3/2012 2:04 PM, Goins, Tresa wrote:
The picric acid around the cap would not be 1%. The acetone
, minimum 2 changes 5 minutes each, and fewer slides in
the rack and some agitation (of the slides, not of you).
If that does not solve the problem incomplete fixation might be the
culprit.Perfused tissue? Fixed how long in ?
Geoff
--
--
**
Geoff
I wonder what my 4th edition (1976) is worth?
Geoff
On 12/7/2011 10:27 AM, Breeden, Sara wrote:
In honor of my impending RETIREMENT on April 1, 2012, I have decided to
offer for sale my copy of Histopathologic Technic and Practical
Histochemistry by R.D. Lillie, 3rd edition (1964
Buehner
et al. J.Histochem. Cytochem. 27(3):782787, 1979 for more thorough
discussion of the chemistry.
And, if a ferrous hematoxylin worked for b-cells we would not need the
chrome alum variety.
Geoff
On 12/1/2011 9:25 AM, Silvina Molinuevo wrote:
Hi Histonetters!!
I want to do Chrome
been used
for many years for EM. Its use has declined as less-toxic alternatives
have been found.
As for immuno, try it, what have you got to lose?
And why did you use cacodylate in the first place?
Geoff
--
--
**
Geoff McAuliffe, Ph.D.
Neuroscience
could
use them for LM.
I will see if I have some references.
Geoff
--
--
**
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcaul...@umdnj.edu
. A comparison of eight
different chromogen protocols for the demonstration of immunoreactive
neurofilaments or glial filaments in rat cerebellum using the
peroxidase-antiperoxidase method and monoclonal antibodies. J.
Histochem. Cytochem. 31:1217-1223.
Geoff
On 10/6/2011 11:49 AM, Tammy Pickles
about an acid alchohol fix, 70 or 80
percent EtOH with 5% acetic acid?
Geoff
On 9/14/2011 7:52 AM, Tyrone Genade wrote:
Hello,
I have been using Bouin's fixative on my fish specimens. I had simply
been killing the fish, slitting them down their belly and letting them
lie in Bouin's overnight
I suspect it will be somewhere between rather ugly to totally worthless,
more likely the latter.
The only was to know is to find out for yourself. Thaw, rinse multiple
times in buffer, osmicate in buffer, rinse, dehydrate, clear and embed
in your favorite epoxy.
Good luck.
Geoff
On 9/13/2011
disappointing, a dull orange not bright red. You might have
better results with the pre-mixed stain. Perhaps they wills send you a
small sample to try on your material.
Geoff
On 8/30/2011 9:15 AM, Vanessa Orsini wrote:
Hi everyone,
I'm Vanessa and i'm quite new in the histology field so i
It may be that Vector has changed the formulation for Hard Set. I
suggest you call them up. I have found their technical support to be
excellent.
Geoff
On 8/29/2011 10:30 AM, Denise G Crowley wrote:
Hi all,
I'm using the Churukian method posted by Gayle Callis a few years ago and I
have
scientific footing.
Geoff
On 7/28/2011 11:28 AM, Harvey, Jennifer Lynn wrote:
I had a investigator say that he is using 10% Buffered Formalin Acetate to fix
his mouse brains. They order it from Fisher (127-09-3). It is buffered with
Sodium Acetate instead. Has anyone ever used this and why would
You can try something called a strap wrench. It is a flexible strap that
wraps around things like oil filters, smoke detectors and jar lids with
a handle on the other end. Available at hardware, Home Depot and maybe
your physical plant dept.
Geoff
On 7/27/2011 12:55 PM, Garcia, Lori, Sr
It is a devil to stain.
Geoff
On 6/30/2011 12:13 PM, Angela Bitting wrote:
ok there is a really BAD religious joke coming soon, isn't there?
Rene J Buesarjbu...@yahoo.com 6/30/2011 12:00 PM
You would have to have an antibody to it and I don't know any one
exists, but I can be wrong.
René
Try interlibrary loan, your library will have access.
Geoff
On 6/8/2011 6:11 PM, Colleen Forster wrote:
Hello Histonetters,
I am wondering if anyone out there has the hard copy volumes of
Methods in Cell Biology Volume 13, pages 171-193 (which is Chapter
9). This chapter has methodology
Suh et al.
I remember that the dogma on paraformaldehyde was that it must be made
fresh. However, I also remember that someone has actually compared fresh
to old (a week or two) and found no difference. This was published in
a peer-reviewed journal, I just don't remember which one.
Geoff
Another possibility is that not every goblet cell is making the same
products at the same time.
Geoff
On 6/4/2011 12:00 PM, mohamed abd el razik wrote:
thanx to all kind replays from sgoebel, Paula Pierce and JR R.
but i know that Alcian blue 2.5 stain most acidic mucins and pH 1 stain
to know if anyone has used this protocol before? Any tips/specific
details might be helpful.
The 20% HCl - does it mean 20% of 37.3% concentrated hydrochloric acid?
Yes.
Any details about acriflavine to be used would be helpful.
Can't help you there.
I'll bet DMAB is very toxic.
Geoff
Thank you
Make the periodic acid fresh that day.
Use 3 2 minute washes in 0.5% sodium meta-bisulfite instead of water.
Geoff
On 3/15/2011 10:16 AM, Martin, Erin wrote:
Hi all,
I'm having a problem with my PAS stain. My pathologist says that glycogen and
fungus stain beautifully but basement membranes
that there are two different
hylaluronidases, bovine testicular and Streptomyces. They have different
activities.
Geoff
GRH National Recognition
Outstanding Rural Health Organization of 2009 awarded by NRHA
Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP
Leader
If you want to get the results of your study published in a reputable
journal you should use the best available method, ie. epoxy sections.
Geoff
On 1/25/2011 5:16 AM, Bataillon Michel wrote:
Hello,
We have to measure the g ratio (ratio of the inner axonal diameter to the
total outer
and it is not contributing to drying out of your tissues.
Those who want methanol-free formalin make it from paraformaldehyde but
for LM there is no point.
Geoff
Jones, Laura wrote:
Greetings to all of you in Histoland. Our lab recently switched from using a
formalin substitute to using 10% Neutral Buffered
Serra's fixative does have water in it, the water is from the
formaldehyde. 37% formaldehyde is water saturated with formaldehyde gas
to about 37%.
The precipitate is probably from the buffering compound, phosphate perhaps.
Geoff
Emily Sours wrote:
A related question:
I was making Serra's
So, the pathologist does not know the answer but he wants to annoy
trainees? Glad I don't work for him!
Geoff
Finlay Finlay wrote:
Hello
Just wondering if anyone knows the what the 'O' in oil red O stands for.
I suspect that it stands for nothing other than O similar to the 'G' in
Orange G
Lead hematoxylin.
See Solcia et al. Histochemie 20:116-126, 1969
or google lead hematoxylin and see what pops up.
Geoff
Lin Bustamante wrote:
Do you know a good specific special stain (not IHC stain) for this cells
of the thyroid gland?
Lin S. Bustamante, B.Sc.; HT(ASCP)
Research Associate
at room temp. is OK for rat and mouse
liver glycogen, I don't know about other species. Slices of liver should
be thin.
When in doubt formalin+alcohol+acetic acid is an excellent fixative.
Geoff
Diana McCaig wrote:
Hi
We have been doing a PAS stain on the same control block and same
reagent
Vector? After all, they made the stuff.
Geoff
Thanks,
-Teresa
--
--
**
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcaul...@umdnj.edu
Merced is correct about longer fixation inducing more cross-linking,
possibly making immunostaining more difficult.
Much depends on the antigen you are looking for.
I disagree that the tissue will become brittle, this is not my experience.
Geoff
Salim Yalcin Inan wrote:
Hello,
I have
.
What happens with less than optimal fixation is the brush border of the
PCT collapses into the lumen, filling it.
Regions that do not have a well developed brush border do not have this
problem, as you see.
Why you are seeing this only now, I do not know.
Geoff
abijag wrote:
Hello
I need your
. formalin (37-40% formaldehyde), 5 ml glacial
acetic acid.
I agree with Rene, Bouin will not make a difference. Alchoholic Bouin is
also worth a try.
Geoff
Itai Moshe wrote:
Dear All,
I'm trying to use liver sections in paraffin blocks for IHC, but get no
signal at all, doesn't matter what antibody
Shrinkage for paraffin processed tissues is about 20%.
For GMA it is less than 5% which is one reason GMA stuff looks so nice.
Geoff
Edwards, Richard E. wrote:
Anybody aware of the degree of shrinkage in paraffin processed tissues and/or
GMA processed tissues?, many thanks
I agree with Rene and Mark.
Geoff
Breeden, Sara wrote:
We're getting ready to move into our new building (YAHOO!) in early
September - finally. In planning where we will store our cases for the
required 6-week retention time, I have proposed that the tissues (in 10%
NBF) be shelved
.
Geoff
mohamed abd el razik wrote:
dear histonetters
i have a quistion please regarding the thickness of lamina propria in small or
larg intestine.
what is the significant of it?? does it mean inflamation and pathological
condition or mean more size and so absorption of nutrients? i mean does
Fifteen to thirty seconds in saturated (or almost saturated), aqueous
picric acid will do it.
Geoff
Mike Tighe wrote:
I have seen Tissue sections counterstained with a yellow counterstain. I would
like to use an Iron hematoxylin with this counter stain. Can anyone tell me
what
Just be sure you get dye certified by the Biological Stain Commission.
The bottle must have a Certification label or it is not certified to
perform as expected.
Sigma is reliable.
Geoff
zodia...@comcast.net wrote:
Hello to all,
I was wondering if anyone knows where to purchase toluidine
. If
there is no certification tag the dye is not certified.
Geoff
Laurie Elmgren wrote:
Hi Everyone,
Lately, we have been having a problem with our Alcian Blue. It is fine
up to the washing step. Once it hits the water, the blue fades to
unacceptable. We have tried reducing the water rinse after the blue
Peter et al.
All people have to do is read the instructions. How much simpler can one
make it? Hit the delete key when you see any sort of unsubscribe message.
Geoff
Peter Carroll wrote:
sometimes, i feel like im stuck in some histonet unsubscribe timewarp. i hate
it!
i really wish
A few drops of saturated aqueous lithium carbonate added to the 70%
alcohol step will do the job nicely.
Geoff
cscam...@uci.edu wrote:
Hi Histonet,
After placing slides in Bouin's for 1 hour at 56 degrees, I am finding it
very difficult to remove the yellow coloring. Rinses with water
Dear all:
aa aa is a troll/spammer. Do not reply to his attempts to cause trouble,
it only encourages him.
Geoff
aa aa wrote:
How Do I unsuscrive from this list please
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http
In addition to Rene's comments, fixation may be an issue. Deeper parts
of the block may not be as well fixed as the more superficial parts so
the farther into the block you go ... :-(
Geoff
Greg Dobbin wrote:
Hi folks,
Has anyone experienced glycogen disappearing from previously
Milligan's Trichrome is reputed to be excellent. I have not used it but
colleagues have.
Milligan, M. A trichrome stain for formalin fixed tissue. Am J. Clin.
Path., Technical Section 10:184-185, 1946.
If you have a copy of Humason's /Animal Tissue Techniques/ the method is
there.
Geoff
with agitation. Then several changes of pure Epon and polymerize.
Yes, you will have to saw away the plastic, if you try to section the
Epon-plastic combo the two will separate.
How much easier do you want it to be?
Geoff
Nicholas David Evans wrote:
Dear all,
I was hoping someone might be able
Your pH meter, unless it is a very sensitive (expensive) meter with a
very good electrode and has been properly calibrated that day is
probably off at least 0.05 - 0.1 pH units so don't worry.
Geoff
Greg Dobbin wrote:
Hi Folks,
If the pH of our 10% Neutral Buffered Formalin is reading 7.01
had left out the O in NaOH. Because she had a full time tech at
her beck and call throughout her PhD training she had never learned how
to make simple solutions. Sad but true.
Geoff
John Kiernan wrote:
This is a typical example of the informal protocols that get passed on from
generation
not store the tissue indefinitely in anything. Do NOT
store the tissue in 70% ethanol, either before or after osmium, ethanol
has been shown to extract cytoplasm from fixed tissues.
The texts John Kiernan recommends are excellent.
Geoff
Nicholas David Evans wrote:
Dear all,
I would like
dichromate. There are many variations on this mix, I doubt if
concentration matters much. Fix sections on slides for an hour or two,
wash well.
I have fixed mouse kidney with osmium-dichromate for paraffin embedding.
The tissue is VERY brittle and hard to cut. I would not expect good results.
Geoff
I suggest you ask for a demo in your lab with your tissue to see if the
machine meets your needs.
Geoff
Edward Roy wrote:
At the Society for Neuroscience meeting I saw a variation on
vibratomes called a Compresstome, by Precisionary Instruments.
Has anyone tried this instrument? They claim
of Shiffs to formaldehyde, not the other
way around.
And, as someone else mentioned, properly cleaned glassware.
Geoff
Lee Peggy Wenk wrote:
Need the help of the Histonet chemistry gurus.
This has happened the last 2 times the students and I made up Schiff. Two
different set of students.
We
Plants have feelings, too.
Geoff
Rene J Buesa wrote:
Isn't out there some purist that could consider that using plants to purify the air from noxious
fumes could be a case of plant cruelty???!!! (Like the canary in the mine?)
René J.
--- On Mon, 10/26/09, Merced M Leiker lei...@buffalo.edu
proceeding.
Geoff
Madary, Joseph wrote:
I have an investigator looking at Massons(with Aniline Blue), Van Gieson
and Gomori's all showing the same type of collagen staining. In some
areas collagen fibers are brilliantly stained and other fibers are pale
all on the same slide. This is consistent
and check the references.
Geoff
Marti Gaudes, Merce wrote:
Hi all.
I need to study gap junctions in heart tissue by electron microscopy. Does
anyone know if I need some specific staining to see the gap junctions?
Thanks a lot.
Mercè Martí Gaudes
Abans d'imprimir aquest
You could but it would be too cold and might crack the tissue.
Isopentane becomes a solid and mitagates, for lack of a better word, the
coldness of liquid nitrogen.
A good alternative would be to cool the rod with dry ice which is only
about -80C or so.
Geoff
Merced M Leiker wrote:
Hi Geoff
Hi Lesley:
There is a fairly large literature on this subject. I my opinion, most
of the fancier stains are not worth the effort and the results are
disappointing. In a separate mailing, I will send you some references.
Geoff
Lesley Bechtold wrote:
Hi Histonetters,
If anyone has a good
. These are NOT the same so be sure of which one is
appropriate for your work..I think the Strep variety is more specific?
Geoff
Amy Lee wrote:
Hello,
I saw a protocol using hyaluronidase digestion for IHC pretreatment. But I couldn't find the way how to make this solution. Could anybody help me please?
Best
If someone leaves the cap off and some of the solvent evaporates you
will have to restore it. Otherwise, straight from the bottle.
Bu the way, DPX is better in many ways.
Geoff
Leslie Chen wrote:
Hello,
Did anyone ever use Permount straight without cutting it with some solvent?
Journal
bookstores that specialize in out of print books. Alibris
comes to mind or just google the title of the book.
Geoff
Joanne Clark wrote:
Is anyone in histoland willing to sell, or know where I can find a copy
of Antigen Retrieval Techniques: Immunohistochemistry Molecular
Morphology by Shi, S
on the fixation and the antigen in question.
Geoff
Jan Shivers wrote:
I'm posting this for a colleague
Does anyone have experience using glycerin/phenol as a storage medium, and if
so, for how long are the tissues still good for Histology processing/staining?
I assume they can't be used for IHC
acid.
Also, yolk is hard to fix well so don't use that as a criterion for good
fixation.
Geoff
Cheryl Crowder wrote:
Hello - A researcher is fixing 7-21 day old chicken embryos in 10% NBF.
After processing they are mushy and almost amorphous. Has anyone any
suggestions for a fixative
Ask the manufacturer, they will know. Or look at the MSDS
Geoff
Merced M Leiker wrote:
Hi all,
What is the difference between Enzyme Grade Tween 20 and Molecular
Biology Grade Tween 20?
Thanks!
Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
if even 40X would be sufficient for hematology.
Geoff
Tiana Fountain wrote:
Hi Everyone,
What are the current thoughts on digital slide imaging systems like
Aperio, etc. I am interested in other people's experience with slide
imaging systems and their preferences.
Any opinions/suggestions
I had a box of low profile blades with the same problem. The vendor sent
me a new box.
Geoff
DiCarlo, Margaret wrote:
Hello,
I just opened a brand new box of Accu-Edge high profile blades and I
cannot get the very first blade to slide out of the container. Even
though I have had the box
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